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https://www.readbyqxmd.com/read/29142048/accurate-pcr-detection-of-influenza-a-b-and-rsv-using-the-cepheid-xpert-flu-rsv-xpress-assay-in-point-of-care-settings-comparison-to-prodesse-proflu
#1
Daniel M Cohen, Jennifer Kline, Larissa S May, Glenn Eric Harnett, Jane Gibson, Stephen Y Liang, Zubaid Rafique, Carina A Rodriguez, Kevin M McGann, Charlotte A Gaydos, Donna Mayne, David Phillips, Jason Cohen
The Xpert Flu+RSV Xpress Assay is a fast, automated in vitro diagnostic test for qualitative detection and differentiation of influenza A, B, and RSV performed on the Cepheid GeneXpert Xpress System. The objective of this study was to establish performance characteristics of the Xpert Flu+RSV Xpress Assay compared to the Prodesse ProFlu+ real-time RT-PCR assay (ProFlu+) for the detection of influenza A and B as well as RSV in a CLIA-waived (CW) setting. Overall, the assay, using fresh and frozen nasopharyngeal (NP) swabs, demonstrated high concordance with ProFlu+ in the combined CW and non-CW settings with Positive Percent Agreements (PPA) (100%, 100%, 97...
November 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29133554/what-is-resistance-impact-of-phenotypic-versus-molecular-drug-resistance-testing-on-multi-and-extensively-drug-resistant-tuberculosis-therapy
#2
Jan Heyckendorf, Sönke Andres, Claudio U Köser, Ioana D Olaru, Thomas Schön, Erik Sturegård, Patrick Beckert, Viola Schleusener, Thomas A Kohl, Doris Hillemann, Danesh Moradigaravand, Julian Parkhill, Sharon J Peacock, Stefan Niemann, Christoph Lange, Matthias Merker
Rapid and accurate drug-susceptibility testing (DST) is essential for the treatment of multi- and extensively drug-resistant tuberculosis (M/XDR-TB). We compared the utility of genotypic DST assays with phenotypic DST (pDST) using BACTEC 960 MGIT or Löwenstein-Jensen to construct M/XDR-TB treatment regimens for a cohort of 25 consecutive M/XDR-TB patients and 15 possible anti-TB drugs.Genotypic DST results from Cepheid GeneXpert MTB/RIF (Xpert) and line probe assays (LPAs: Hain GenoType MTBDRplus 2.0 and MTBDRsl 2...
November 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29117351/finding-the-missing-patients-with-tuberculosis-lessons-learned-from-patient-pathway-analyses-in-5-countries
#3
Christy Hanson, Mike Osberg, Jessie Brown, George Durham, Daniel P Chin
Background: Despite significant progress in diagnosis and treatment of tuberculosis over the past 2 decades, millions of patients with tuberculosis go unreported every year. The patient-pathway analysis (PPA) is designed to assess the alignment between tuberculosis care-seeking patterns and the availability of tuberculosis services. The PPA can help programs understand where they might find the missing patients with tuberculosis. Methods: This analysis aggregates and compares the PPAs from case studies in Kenya, Ethiopia, Indonesia, the Philippines, and Pakistan...
November 6, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29097418/prevalence-and-treatment-outcomes-of-routine-chlamydia-trachomatis-neisseria-gonorrhoeae-and-trichomonas-vaginalis-testing-during-antenatal-care-gaborone-botswana
#4
Adriane Wynn, Doreen Ramogola-Masire, Ponatshego Gaolebale, Neo Moshashane, Ontiretse Sickboy, Sofia Duque, Elizabeth Williams, Klara Doherty, Jeffrey D Klausner, Chelsea Morroni
OBJECTIVES: Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) are curable, mostly asymptomatic, STIs that cause adverse maternal and perinatal outcomes. Most countries do not test for those infections during antenatal care. We implemented a CT, NG and TV testing and treatment programme in an antenatal clinic in Gaborone, Botswana. METHODS: We conducted a prospective study in the antenatal clinic at Princess Marina Hospital in Gaborone, Botswana...
November 2, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29091135/an-evaluation-of-false-positive-rifampicin-resistance-on-the-xpert-mtb-rif
#5
Yeliz Tanriverdi Cayci, Kemal Bilgin, Ahmet Yilmaz Coban, Asuman Birinci, Belma Durupınar
BACKGROUND: Mycobacterium tuberculosis (MTB) is one of the most significant causes of mortality and morbidity. Early diagnose is important especially in multiple drug resistant tuberculosis to avoid transmission. Traditional techniques requires at least one to three weeks for diagnosis of tuberculosis. Diagnostic delays with multiple drug resistant tuberculosis are associated with worse clinical outcomes and increased transmission The Xpert MTB/RIF assay is one of the new diagnostic device for the diagnosis of tuberculosis and rapid detection of rifampicin resistance...
November 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29066627/detection-of-neisseria-gonorrhoeae-and-chlamydia-trachomatis-from-pooled-rectal-pharyngeal-and-urine-specimens-in-men-who-have-sex-with-men
#6
David John Speers, I-Ly Joanna Chua, Justin Manuel, Lewis Marshall
OBJECTIVES: Screening of men who have sex with men (MSM) for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) requires sampling from anorectal and pharyngeal sites in addition to urogenital sampling. Due to the cost of testing multiple anatomical sites individually testing of pooled specimens has potential merit. The Cepheid GeneXpert CT/NG assay (GeneXpert), which also has potential for point-of-care nucleic acid testing in the sexual health clinic, has not been assessed for pooled specimen testing...
October 24, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29062564/bacille-calmette-gu%C3%A3-rin-vaccine-induced-tuberculous-hip-osteomyelitis-in-an-infant-case-report
#7
Mohammed S Al-Azri, Jazel Manarang, Yaqoub Al-Mufargi
To this day, tuberculosis (TB) continues to pose a significant global health burden. The World Health Organization's Expanded Programme on Immunization (EPI) recommends the bacille Calmette-Guérin (BCG) vaccine for infants to protect against the haematogenous spread of primary TB and other more severe types of TB infection. We report an eight-month-old boy who presented to the Armed Forces Hospital, Muscat, Oman, in 2015 with a one-month history of intermittent fever associated with a limited range of motion in the right hip area...
August 2017: Sultan Qaboos University Medical Journal
https://www.readbyqxmd.com/read/29061538/development-of-a-90-minute-integrated-non-invasive-urinary-assay-for-bladder-cancer-detection
#8
Ellen Wallace, Russell Higuchi, Malini Satya, Leena McCann, Mandy Ly Sin, Julia A Bridge, Huilin Wei, Jun Zhang, Edith Wong, Andrew Hiar, Kathleen E Mach, Douglas Scherr, R Blair Egerdie, Shoichiro Ohta, Wade J Sexton, Maxwell V Meng, Alon Z Weizer, Michael Woods, G Kenneth Jansz, Joseph Zadra, Yair Lotan, Joseph C Liao
PURPOSE: Despite suboptimal sensitivity, urine cytology is often performed as an adjunct to cystoscopy for bladder cancer diagnosis. We aimed to develop a non-invasive, fast molecular diagnostic test for bladder cancer detection with better sensitivity than urine cytology while maintaining adequate specificity. MATERIALS AND METHODS: Urine specimens were collected at 18 multinational sites from subjects prior to cystoscopy or tumor resection, and from healthy and other control subjects without evidence of bladder cancer...
October 20, 2017: Journal of Urology
https://www.readbyqxmd.com/read/29038191/obstructive-uropathy-as-initial-presentation-of-genitourinary-tuberculosis-and-masquerading-as-a-postsurgical-complication
#9
Grace Igiraneza, Theobald Hategekimana, Olivier M Manzi, Onyema Ogbuagu
A 46-year-old woman who had a recent total abdominal hysterectomy presented with a 1 month history of lower abdominal pain, 1 week of nausea and vomiting as well as decreased urinary output preceded by a year of significant unintentional weight loss. On renal imaging, she was found to have bilateral hydronephrosis and hydroureters in the setting of bilateral distal ureteric obstruction complicated with acute kidney injury and severe hyperkalaemia requiring haemodialysis. The initial concern was for ureteric injury, a known complication of abdominal hysterectomy procedures, however, a urological intervention, performed 9 months later to relieve the ureteric obstruction, revealed purulent material within the left ureter that was smear positive for acid fast bacilli...
October 15, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29028228/preventing-emergence-of-drug-resistant-tuberculosis-in-myanmar-s-transitioning-health-system
#10
Mishal S Khan, Coll Hutchison, Richard J Coker, Joanne Yoong, Khaung M Hane, Anh L Innes, Tin M Khaing, Sithu Aung
Multidrug-resistant tuberculosis (MDR-TB) is a particular threat to the populations of resource-limited countries. Although inadequate treatment of TB has been identified as a major underlying cause of drug resistance, essential information to inform changes in health service delivery and policy is missing. We investigate factors that may be driving the emergence of MDR-TB in Myanmar, a country where investment and health system reforms are ongoing to address the unexplained, high occurrence of MDR-TB. We conducted a multi-centre, retrospective case-control study in 10 townships across Yangon...
October 1, 2017: Health Policy and Planning
https://www.readbyqxmd.com/read/29026744/profile-of-drug-resistant-tuberculosis-in-western-maharashtra
#11
Sudhakar W More, Malangori Abdulgani Parande, Sanjeev W Kamble, Manjunath S Kamble
CONTEXT: Drug-resistant tuberculosis (TB) strains have evolved mainly due to incomplete or improper treatment of TB patients and are one of the hurdles in controlling TB problem. It is better to understand the magnitude and comorbidities associated with drug-resistant TB. AIM AND OBJECTIVES: (1) To study some of the sociodemographic profile and history of TB treatment of drug-resistant TB cases. (2) To study their drug-resistance pattern and their comorbidity profile...
January 2017: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/29022528/diagnostic-accuracy-of-genexpert-mtb-rif-in-musculoskeletal-tuberculosis-high-sensitivity-in-tissue-samples-of-hiv-infected-and-hiv-uninfected-patients
#12
M Held, M Laubscher, L Workman, H J Zar, R Dunn
BACKGROUND: GeneXpert MTB/RIF is useful for the diagnosis of pulmonary TB in adults, but there is limited evidence on its usefulness in extrapulmonary TB. OBJECTIVES: To investigate the diagnostic accuracy of GeneXpert MTB/RIF in HIV-infected and HIV-uninfected patients with suspected musculoskeletal TB. METHODS: A prospective study of patients with suspected musculoskeletal (bone and joint) TB was undertaken. The diagnostic accuracy of GeneXpert MTB/RIF was compared with the reference standards of culture and histopathology...
September 22, 2017: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/29019524/-budget-impact-of-the-incorporation-of-genexpert-mtb-rif-for-diagnosis-of-pulmonary-tuberculosis-from-the-perspective-of-the-brazilian-unified-national-health-system-brazil-2013-2017
#13
Márcia Ferreira Teixeira Pinto, Ricardo Steffen, Aline Entringer, Ana Carolina Carioca da Costa, Anete Trajman
The study aimed to estimate the budget impact of GeneXpert MTB/RIF for diagnosis of tuberculosis from the perspective of the Brazilian National Program for Tuberculosis Control, drawing on a static model using the epidemiological method, from 2013 to 2017. GeneXpert MTB/RIF was compared with two diagnostic sputum smear tests. The study used epidemiological, population, and cost data, exchange rates, and databases from the Brazilian Unified National Health System. Sensitivity analysis of scenarios was performed...
October 9, 2017: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/28968017/-diagnostic-accuracy-of-three-technologies-for-the-diagnosis-of-multi-drug-resistant-tuberculosis
#14
Nelson José Alvis-Zakzuk, María De Los Ángeles Carrasquilla, Verónica Jhajaira Gómez, Jaime Robledo, Nelson Rafael Alvis-Guzmán, José Mauricio Hernández
INTRODUCTION: Multi-drug resistant (MDR-TB) and extensively drug-resistant (XDR-TB) tuberculoses are a global public health problem. Their timely detection might reduce the burden of the disease and the economic impact on health systems worldwide. OBJECTIVE: To conduct a literature review of the diagnostic accuracy of three molecular tests to detect multi-drug resistant and extensively drug-resistant tuberculoses. MATERIALS AND METHODS: A systematic literature review following Cochrane methodology was carried out to study the diagnostic accuracy of three molecular tests to detect MDR-TB and XDR-TB in previous studies among immunocompetent population...
September 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28950760/xpert-flu-as-a-rapid-diagnostic-test-for-respiratory-tract-viral-infection-evaluation-and-implementation-as-a-24-7-service
#15
Sofie Haglund, Maysae Quttineh, Annette Nilsson Bowers, Andreas Matussek, Anna J Henningsson
A rapid and reliable influenza method is important to meet diagnostic needs. Here, the performance of the cartridge-based Xpert Flu assay was evaluated in comparison with an in-house real-time PCR method. Analysis was then decentralized from the clinical microbiology laboratory located at County Hospital Ryhov, Jönköping, Sweden, to the clinical chemistry laboratories present at the three hospitals in Region Jönköping County, allowing a 24/7 service. In total, 189 clinical samples were included in 2014-2015...
September 26, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28944144/osteomyelitis-due-to-multiple-rare-infections-in-a-patient-with-idiopathic-cd4-lymphocytopenia
#16
Nitin Gupta, Sayantan Banerjee, Timitrov, Rohini Sharma, Shambo Guha Roy, Trupti M Shende, Mohammed Tahir Ansari, Gagandeep Singh, Neeraj Nischal, Naveet Wig, Manish Soneja
A 26-year-old male patient presented with features suggestive of osteomyelitis involving the entire left femur, hip joint and knee joint. Culture from the debrided tissue grew Acinetobacter spp. and he was treated with sensitivity based antibiotics but the symptoms did not resolve. The synovial biopsy showed multinucleated giant cells and acid fast bacilli on Ziehl Neelsen stain. Cartridge based nucleic acid amplification test (GeneXpert) was negative. The Mycobacteria growth indicator tube culture was found to be positive for Mycobacterium abscessus...
August 2017: Intractable & Rare Diseases Research
https://www.readbyqxmd.com/read/28938874/rifampicin-resistance-in-mycobacterium-tuberculosis-patients-using-genexpert-at-livingstone-central-hospital-for-the-year-2015-a-cross-sectional-explorative-study
#17
Sepiso K Masenga, Harrison Mubila, Benson M Hamooya
BACKGROUND: Since the recent introduction of GeneXepert for the detection of Tuberculosis (TB) drug resistance mutations in both primary resistance and acquired resistance in Zambia, little has been documented in literature on the issue of rifampicin resistance especially in the face of a high National TB burden. The study aimed to determine the prevalence of rifampicin resistance in tuberculosis patients at Livingstone Central Hospital for the year 2015. METHODS: This was a cross sectional study conducted at Livingstone Central Hospital where we reviewed 152 records (from January 1, 2015 to 31st December, 2015) involving patients who presented with clinically suspected TB or documented TB, whose samples were sent to the laboratory for GeneXpert Mycobacterium tuberculosis/rifampicin testing...
September 22, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28936998/venous-thromboembolism-due-to-hyperhomocysteinaemia-and-tuberculosis
#18
Ankush Chaudhary, Unnati Desai, Jyotsna M Joshi
An 18-year-old male presented to our hospital with complaints of episodic abdominal pain, dry cough and right pleuritic chest pain. He was diagnosed as a case of right tuberculous pleural effusion on the basis of the pleural fluid Genexpert report of Mycobacterium tuberculosis detected sensitive to rifampicin and was started on antituberculous therapy. Forty-five days later, he presented with acute onset breathlessness, swelling of the right leg, streaky haemoptysis and a fresh left-sided pleural effusion. Evaluation revealed venous thromboembolism (right lower lobar segment pulmonary embolism with right leg deep vein thrombosis)...
May 2017: National Medical Journal of India
https://www.readbyqxmd.com/read/28934422/low-frequency-of-acquired-isoniazid-and-rifampicin-resistance-in-rifampicin-susceptible-pulmonary-tuberculosis-in-a-setting-of-high-hiv-1-infection-and-tuberculosis-coprevalence
#19
Neesha Rockwood, Frederick Sirgel, Elizabeth Streicher, Robin Warren, Graeme Meintjes, Robert J Wilkinson
Background: We estimated the incidence of acquired isoniazid and rifampicin resistance in rifampicin-susceptible tuberculosis in a setting of high human immunodeficiency virus type 1 (HIV-1) infection and tuberculosis coprevalence. Methods: GeneXpert MTB/RIF-confirmed patients with rifampicin-susceptible tuberculosis were recruited at antituberculosis treatment initiation in Khayelitsha, South Africa. Liquid culture and adherence assessment were performed at 2 and 5-6 months...
September 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28931561/genetic-mimetics-of-mycobacterium-tuberculosis-and-methicillin-resistant-staphylococcus-aureus-as-verification-standards-for-molecular-diagnostics
#20
Edith Erika Machowski, Bavesh Davandra Kana
Molecular diagnostics have revolutionized the management of health care through enhanced detection of disease or infection and effective engagement into care. In recognition of this, the World Health Organization approved the rollout out of nucleic acid amplification technologies for identification of Mycobacterium tuberculosis using platforms such as GeneXpert MTB/RIF, the GenoType MTBDRplus Line Probe Assay and more recently, GeneXpert MTB/RIF Ultra. These assays can simultaneously detect tuberculosis infection and assess rifampicin resistance...
September 20, 2017: Journal of Clinical Microbiology
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