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https://www.readbyqxmd.com/read/28438221/isolated-splenic-tuberculosis-with-subsequent-paradoxical-deterioration-a-case-report
#1
Frederick Wangai, Loice Achieng, George Otieno, Jacqueline Njoroge, Tabitha Wambaire, Jamilla Rajab
BACKGROUND: Isolated tuberculosis of the spleen has been described occasionally in literature, mostly in immunosuppressed individuals with various risk factors. Sequestration in the spleen makes such Mycobacterium tuberculosis infection difficult to diagnose. This report describes an extremely rare case of isolated splenic tuberculosis in an immunocompetent individual. CASE PRESENTATION: A 26 year old Kenyan male presented with pyrexia of unknown origin, with negative screening tests for bacterial, fungal and parasitic infections...
April 24, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28410700/genexpert-a-momentous-innovation-that-needs-a-touch-of-prudence
#2
EDITORIAL
Mandira Varma-Basil, Ashok Shah
No abstract text is available yet for this article.
April 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28399817/assessments-of-serum-copper-and-zinc-concentration-and-the-cu-zn-ratio-determination-in-patients-with-multidrug-resistant-pulmonary-tuberculosis-mdr-tb-in-c%C3%A3-te-d-ivoire
#3
Gnogbo Alexis Bahi, Lydie Boyvin, Souleymane Méité, Gervais Melaine M'Boh, Kadjowely Yeo, Kouassi Raymond N'Guessan, Alain Dit Philippe Bidié, Allico Joseph Djaman
BACKGROUND: In Côte d'Ivoire, multidrug-resistant tuberculosis (MDR-TB) is a serious public health problem with a prevalence estimated at 2.5% in 2006. Zinc and copper are essential Trace element needed to strengthen the immune system and also useful in the fight against tuberculosis. The Cu / Zn ratio is a good indicator of oxidative stress. The principal aim of this study was to evaluate the serum concentration of some trace element and determine the Cu / Zn ratio in patients with multidrug resistant pulmonary tuberculosis (MDR-TB) before and after second line treatment of TB...
April 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28362869/high-prevalence-of-curable-sexually-transmitted-infections-among-pregnant-women-in-a-rural-county-hospital-in-kilifi-kenya
#4
Simon Chengo Masha, Elizabeth Wahome, Mario Vaneechoutte, Piet Cools, Tania Crucitti, Eduard J Sanders
BACKGROUND: Women attending antenatal care (ANC) in resource-limited countries are frequently screened for syphilis and HIV, but rarely for other sexually transmitted infections (STIs). We assessed the prevalence of curable STIs, defined as infection with either Chlamydia trachomatis or Neisseria gonorrhoeae or Trichomonas vaginalis, from July to September 2015. METHODS: In a cross-sectional study, women attending ANC at the Kilifi County Hospital, Kenya, had a urine sample tested for C...
2017: PloS One
https://www.readbyqxmd.com/read/28358797/evaluation-of-automated-molecular-testing-rollout-for-tuberculosis-diagnosis-using-routinely-collected-surveillance-data-uganda-2012-2015
#5
Colleen Scott, Simon Walusimbi, Bruce Kirenga, Moses Joloba, Muttamba Winters, Nyombi Abdunoor, Rommel Bain, Heather Alexander, Tom Shinnick, Sean Toney, Rosemary Odeke, Christine Mwangi, Estella Birabwa, Seyoum Dejene, Frank Mugabe, Mukadi YaDiul, J Sean Cavanaugh
In 2012, Uganda introduced the use of GeneXpert MTB/RIF (Cepheid, Sunnyvale CA), a sensitive, automated, real-time polymerase chain reaction-based platform for tuberculosis (TB) diagnosis, for programmatic use among children, adults with presumptive human immunodeficiency virus (HIV)-associated TB, and symptomatic persons at risk for rifampicin (RIF)-resistant TB. The effect of using the platform's Xpert MTB/RIF assay on TB care and control was assessed using routinely collected programmatic data; in addition, a retrospective review of district quarterly summaries using abstracted TB register data from purposively selected facilities in the capital city of Kampala was conducted...
March 31, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28351283/evaluation-of-genexpert-mtb-rif-for-detecting-mycobacterium-tuberculosis-in-a-hospital-in-china
#6
Tingyu Tang, Fang Liu, Xiaoling Lu, Qingdong Huang
Objective To evaluate the performance of GeneXpert MTB/RIF in diagnosing pulmonary tuberculosis (TB) in China. Methods This cross-sectional study included sputum specimens of 240 suspected TB cases. Specimens were examined by light microscopy for the presence of acid-fast bacilli, which were cultured by the BACTEC MGIT 960 (M960) system and detected by the GeneXpert MTB/RIF assay. The positive rate, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and average turnaround time of methods were evaluated...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28321579/performance-of-three-generations-of-xpert-mrsa-in-routine-practice-approaching-the-aim
#7
H Jacqmin, A Schuermans, S Desmet, J Verhaegen, V Saegeman
The aim of this study was to evaluate retrospectively the performance of the Xpert MRSA assay in routine practice and its current use in the intensive care unit (ICU) setting of our hospital, since a pre-emptive isolation strategy has been applied. A total of 6473 patients were routinely screened with ESwab for methicillin-resistant Staphylococcus aureus (MRSA) using three generations of rapid real-time polymerase chain reaction (PCR) (Cepheid GeneXpert) over three consecutive periods of time. Performance was evaluated using broth enrichment culture as the reference method...
March 20, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28317800/are-who-approved-nucleic-acid-amplification-tests-causing-large-scale-false-identification-of-rifampicin-resistant-tuberculosis-programmatic-experience-from-south-india
#8
Praveen Sanker, Anusree P Ambika, Vishnu T Santhosh, Ramya Puthukkudi Kottuthodi, Ravikrishnan Balakrishnan, Sunil Kumar Mrithunjayan, Hisham Moosan
INTRODUCTION: The nucleic acid amplification tests (NAATs): Line probe assay and GeneXpert (Xpert) have evolved as the primary tool for identification of rifampicin (RIF)-resistant (RR) tuberculosis (TB) worldwide, primarily because of the ease and speed. We rechecked RR isolates identified by NAATs from presumptive RR TB cases belonging to South India by the Revised National TB Control Program, India using multiple RIF concentrations on Bactec MGIT system and compared the mutation patterns with the resistance levels...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28278193/a-bcr-abl1-cutoff-of-1-5-at-3-months-determined-by-the-genexpert-system-predicts-an-optimal-response-in-patients-with-chronic-myeloid-leukemia
#9
Valentín García-Gutiérrez, María T Gómez-Casares, José M Puerta, Juan M Alonso-Domínguez, Santiago Osorio, Juan C Hernández-Boluda, Rosa Collado, María J Ramírez, Fátima Ibáñez, María L Martín, Juan D Rodríguez-Gambarte, Carolina Martínez-Laperche, Montse Gómez, Dolly V Fiallo, Sara Redondo, Alicia Rodríguez, Concepción Ruiz-Nuño, Juan L Steegmann, Antonio Jiménez-Velasco
In chronic myeloid leukemia (CML) patients, 3-month BCR-ABL1 levels have consistently been correlated with further outcomes. Monitoring molecular responses in CML using the GeneXpert (Cepheid) platform has shown an optimal correlation with standardized RQ-PCR (IS) when measuring BCR-ABL1 levels lower than 10%, as it is not accurate for values over 10%. The aim of the present study was to determine the predictive molecular value at three months on different outcome variables using the Xpert BCR-ABL1 MonitorTM assay (Xpert BCR-ABL1)...
2017: PloS One
https://www.readbyqxmd.com/read/28258004/evaluation-of-genexpert-mtb-rif-for-detection-of-pulmonary-tuberculosis-at-peripheral-tuberculosis-clinics
#10
Yan Shao, Hong Peng, Cheng Chen, Tao Zhu, Ming Ji, Wei Jiang, Wei Zhu, Xiang Jun Zhai, Wei Lu
Tuberculosis is one of the most common infectious diseases in China, while delayed patient finding obstructed disease control, especially for smear-negative patients. The current study was undertaken to evaluate the diagnostic accuracy of GeneXpert MTB/RIF compared with conventional methods in the detection of pulmonary tuberculosis patients. A total of 295 spot sputum samples from confirmed pulmonary tuberculosis patients were evaluated from September 2014 to June 2015. Each sample was examined by acid-fast bacillus smear microscopy, culture and GeneXpert MTB/RIF...
February 28, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28247256/-rare-cutaneous-tuberculosis-in-sub-saharan-africa-developed-on-discoid-lupus-erythematous-lesion
#11
A Diop, M T Ndiaye, M Ndiaye, B Seck, A Diouf, B H Diatta, M Diallo, F Ly
Lupus vulgaris is a common presentation of cutaneous tuberculosis (TB), but its ulcerative or vegetating form also called vorax is rare.We report a case of lupus vulgaris in its vorax form, which occurred in a patient with discoid lupus erythematous. A 42-year-old patient monitored for chronic lupus erythematosus consulted again for a facial tumor and fever. Physical examination revealed painful ulcerative and crusted lesions on an old discoid lupus lesion and covered the entire upper lip. Similar lesions were noted on cheeks...
February 28, 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/28193202/association-of-high-serum-vitamin-d-concentrations-with-active-pulmonary-tb-in-an-hiv-co-endemic-setting-harare-zimbabwe
#12
Cuthbert Musarurwa, Lynn Sodai Zijenah, Doreen Zvipo Duri, Kudzie Mateveke-Dangaiso, Kudakwashe Mhandire, Maria Mary Chipiti, Marshall Wesley Munjoma, Witmore Bayayi Mujaji
BACKGROUND: There is paucity data on the association of vitamin D deficiency (VDD) and active tuberculosis (TB) in southern Africa where the human immunodeficiency virus (HIV) is co-endemic. We examined the association of serum vitamin D concentrations with active pulmonary tuberculosis (PTB) in HIV-infected (n = 284) and uninfected (n = 267) Black Zimbabweans, in Harare, Zimbabwe. METHODS: We conducted a cross-sectional study of 551 participants comprising 145 HIV(+)/PTB (+), 139 HIV(+)/PTB(-), 134 HIV(-)/PTB(+) and 133 HIV(-)/PTB(-)...
February 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28185823/variation-in-mrsa-identification-results-from-different-generations-of-xpert-mrsa-real-time-pcr-testing-kits-from-nasal-swabs
#13
Ali A Rabaan, Ali M Bazzi
GeneXpert MRSA kits (Cepheid) are based on a multiplex, real-time PCR method for methicillin-resistant Staphylococcus aureus (MRSA) detection, with primers to detect each SCCmec type and the chromosomal orfX-SCCmec junction. Modifications in recent kit versions were proposed to help overcome false-positive issues in earlier kit versions. The main objective of this study was to determine whether use of any version of the GeneXpert MRSA multiplex, real-time PCR kits yielded higher than expected MRSA+ results...
February 6, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28182591/comparison-of-cobas-4800-m2000-viper-xtr-and-infinity-80-automated-instruments-when-processing-urine-specimens-for-the-diagnosis-of-chlamydia-trachomatis-and-neisseria-gonorrhoeae
#14
Max A Chernesky, Dan Jang, Jodi Gilchrist, Marek Smieja, Manuel Arias, Todd Hatchette, Andre Poirier, Donna Mayne, Sam Ratnam
OBJECTIVES: North American and European advisory groups recommend testing for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) with nucleic acid amplification tests. Testing is often performed on automated instruments. The objectives of this study were to process urines for the diagnosis of CT and NG and to examine workflow procedures and outcomes. METHODS: While processing 1, 24, 48, 96, and 192 urine specimens on 3 batch-mode systems which use 96-well plates: cobas 4800, m2000, and Viper XTR and the random access cartridge testing GeneXpert Infinity 80, we measured assay performance, hands-on time for processing and maintenance, reagents and plastics consumption, time required to obtain results, and testing accuracy...
March 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28166918/the-concomitant-occurrence-of-pulmonary-tuberculosis-with-bronchial-anthracofibrosis
#15
Shekhar Kunal, Ashok Shah
BACKGROUND: Bronchial anthracofibrosis (BAF), diagnosed bronchoscopically, is a clinical entity which is now beginning to emerge from obscurity. This is commonly encountered in elderly females with history of long-standing exposure to biomass fuel smoke in poorly ventilated kitchens. As awareness of BAF has increased in recent times, distinct clinicoradiological and bronchoscopic features of the disease have emerged. Diagnosis is achieved by visualisation of bluish-black mucosal hyperpigmentation along with narrowing/distortion of the affected bronchus on fibreoptic bronchoscopy (FOB)...
January 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28160728/point-of-care-testing-poct-in-molecular-diagnostics-performance-evaluation-of-genexpert-hcv-rna-test-in-diagnosing-and-monitoring-of-hcv-infection
#16
Ekta Gupta, Pragya Agarwala, Guresh Kumar, Rakhi Maiwall, Shiv Kumar Sarin
BACKGROUND: Molecular testing at the point-of-care may turn out to be game changer for HCV diagnosis and treatment monitoring, through increased sensitivity, reduced turnaround time, and ease of performance. One such assay GeneXpert(®) has recently been released. OBJECTIVES: Comparative analysis between performances of GeneXpert(®) and Abbott HCV-RNA was done. STUDY DESIGN: 174 HCV infected patients were recruited and, one time plasma samples from 154 patients and repeated samples from 20 patients, obtained at specific treatment time-points (0, 4, 12 and 24) weeks were serially re-tested on Xpert(®)...
March 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28159916/impact-of-deploying-multiple-point-of-care-tests-with-a-sample-first-approach-on-a-sexual-health-clinical-care-pathway-a-service-evaluation
#17
Emma M Harding-Esch, Achyuta V Nori, Aseel Hegazi, Marcus J Pond, Olanike Okolo, Anthony Nardone, Catherine M Lowndes, Phillip Hay, S Tariq Sadiq
OBJECTIVES: To assess clinical service value of STI point-of-care test (POCT) use in a 'sample first' clinical pathway (patients providing samples on arrival at clinic, before clinician consultation). Specific outcomes were: patient acceptability; whether a rapid nucleic acid amplification test (NAAT) for Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) could be used as a POCT in practice; feasibility of non-NAAT POCT implementation for Trichomonas vaginalis (TV) and bacterial vaginosis (BV); impact on patient diagnosis and treatment...
February 3, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28155817/impact-of-the-genexpert-mtb-rif-technology-on-tuberculosis-control
#18
Wendy Susan Stevens, Lesley Scott, Lara Noble, Natasha Gous, Keertan Dheda
Molecular technology revolutionized the diagnosis of tuberculosis (TB) with a paradigm shift to faster, more sensitive, clinically relevant patient care. The most recent molecular leader is the GeneXpert MTB/RIF assay (Xpert) (Cepheid, Sunnyvale, CA), which was endorsed by the World Health Organization with unprecedented speed in December 2010 as the initial diagnostic for detection of HIV-associated TB and for where high rates of drug resistance are suspected. South Africa elected to take an aggressive smear replacement approach to facilitate earlier diagnosis and treatment through the decision to implement the Xpert assay nationally in March 2011, against the backdrop of approximately 6...
January 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28138184/genexpert-a-new-tool-for-the-rapid-detection-of-rifampicin-resistance-in-mycobacterium-tuberculosis
#19
Muhammad Saeed, Shagufta Iram, Shahida Hussain, Adeel Ahmed, Mamoon Akbar, Maleeha Aslam
OBJECTIVE: To evaluate the diagnostic accuracy of GeneXpert assay for the detection of rifampicin resistance in mycobacterium tuberculosis using conventional drug susceptibility testing as gold standard. METHODS: This cross-sectional study was conducted at Jinnah Hospital, Lahore, / Allama Iqbal Medical College, Lahore, Pakistan, from January 2012 to December 2014, and comprised clinically and radiologically diagnosed tuberculosis suspected cases. Pulmonary and extra-pulmonary specimens were collected from strong tuberculosis suspects...
February 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28125981/smear-positive-pulmonary-tuberculosis-and-associated-risk-factors-among-tuberculosis-suspects-attending-spiritual-holy-water-sites-in-northwest-ethiopia
#20
Dejene Derseh, Feleke Moges, Belay Tessema
BACKGROUND: Tuberculosis (TB) remains one of the world's deadliest communicable diseases. In Ethiopia, tuberculosis patients have different pattern of health care seeking behavior. They usually adopt other approaches like traditional healers and spiritual holy water sites before consulting public health facilities. This study was aimed to assess the prevalence of smear positive pulmonary tuberculosis and associated risk factors among tuberculosis suspects attending spiritual holy water sites...
January 26, 2017: BMC Infectious Diseases
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