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https://www.readbyqxmd.com/read/28630849/a-case-of-chronic-inflammation-of-the-ankle-joint-with-subtle-signs-of-inflammation-a-rare-presentation-of-tuberculosis-ankle
#1
Jeevan Pereira, S Anoop, George Joy Pettah
INTRODUCTION: Tuberculosis (TB) of the ankle is an uncommon presentation of extrapulmonary infection. The early diagnosis of TB arthritis of the ankle is difficult because of the uncommon site, the ability of TB to mimic other disorders and, all too common, lack of awareness of about this infection in ankle joint. CASE REPORT: A 30-year-old male presented with a 1-year history of right ankle pain and swelling. He denied any history of recent infection but had had recurrent ankle pain that had been treated accordingly...
January 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28615471/clostridium-difficile-pcr-cycle-threshold-predicts-free-toxin
#2
Fiona Senchyna, Rajiv L Gaur, Saurabh Gombar, Cynthia Y Truong, Lee F Schroeder, Niaz Banaei
Background. There is no stand-alone Clostridium difficile diagnostic that can sensitively and rapidly detect fecal free toxins. We investigated the performance of C. difficile PCR cycle threshold (CT) for predicting free toxin status.Method. Consecutive stool samples (n=312) positive for toxigenic C. difficile with the GeneXpert C.diff Epi tcdB PCR assay were tested with the rapid membrane C. DIFF QUIK CHEK COMPLETE immunoassay (RMEIA). RMEIA toxin-negative samples were tested with the cell cytotoxicity neutralization assay (CCNA) and tgcBIOMICS ELISA...
June 14, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28612135/comparative-study-of-genexpert-mtb-rif-assay-and-multiplex-pcr-assay-for-direct-detection-of-mycobacterium-tuberculosis-in-suspected-pulmonary-tuberculosis-patients
#3
Anil Kumar Sah, Bishnu Joshi, Dhruba Kumar Khadka, Birendra Prasad Gupta, Anurag Adhikari, Subodh Kant Singh, Ganesh Rai, Geeta Shrestha Vaidya, Reema Rajbhandari, Basant Pant, Shiba Kumar Rai
Pulmonary tuberculosis (PTB) is one of the major infectious diseases in developing countries. The objective of this study was to compare rapid diagnostics technique, GeneXpert MTB/RIF (GeneXpert) and Multiplex PCR assay (MPCR) targeting IS6110 segment and mpb64 gene for direct detection of Mycobacterium tuberculosis (MTB) in suspected PTB patients. A cross sectional study was carried among 105 sputum samples from suspected PTB patients to evaluate GeneXpert and Multiplex PCR who visited National Tuberculosis Center, Nepal...
June 13, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28559923/prevalence-and-risk-factors-associated-with-sexually-transmitted-infections-stis-among-women-of-reproductive-age-in-swaziland
#4
Themba G Ginindza, Cristina D Stefan, Joyce M Tsoka-Gwegweni, Xolisile Dlamini, Pauline E Jolly, Elisabete Weiderpass, Nathalie Broutet, Benn Sartorius
BACKGROUND: Sexually transmitted infections (STIs) remain an important public health problem with approximately half a billion new cases annually among persons aged 15-49 years. Epidemiological data on STIs among women of reproductive age in Swaziland are limited. The availability of epidemiological data on STIs and associated risk factors in this population is essential for the development of successful prevention, diagnosis and management strategies in the country. The study aimed to determine the prevalence and risk factors associated with STIs...
2017: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/28516818/the-changing-epidemiology-of-spinal-tuberculosis-the-influence-of-international-immigration-in-catalonia-1993-2014
#5
M Peghin, D Rodriguez-Pardo, A Sanchez-Montalva, F Pellisé, A Rivas, T Tortola, J Aguilar, B Almirante, C Pigrau
The overall incidence of spinal tuberculosis (TB) appears to be stable or declining in most European countries, but with an increasing proportion of cases in the foreign-born populations. We performed a retrospective observational study (1993-2014), including all cases of spinal TB diagnosed at a Barcelona hospital to assess the epidemiological changes. Fifty-four episodes (48·1% males, median age 52 years) of spinal TB were diagnosed. The percentage of foreign-born residents with spinal TB increased from 14% to 45·2% in the last 10 years (P = 0·017)...
May 18, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28501582/molecular-detection-of-rifampicin-resistance-by-genexpert-%C3%A2-assay-among-treated-and-untreated-pulmonary-tuberculosis-patients-from-khyber-pakhtunkhwa-pakistan
#6
Hazir Rahman, Shakir Ullah Khan, Muhamamd Amjad Khan, Muhammad Qasim, Abdul Jabbar, Sadiq Noor, Zakir Khan, Taj Ali Khan, Mubbashir Hussain, Noor Muhammad, Nawab Ali
OBJECTIVES: Drug-resistant tuberculosis (TB) is an emerging health problem. Rifampicin (RIF) is the major first-line drug against TB. RIF resistance can be used as a marker for the detection of multidrug-resistant TB (MDR-TB). The purpose of this study was to determine the RIF resistance pattern of Mycobacterium tuberculosis complex isolates among treated and untreated patients in Khyber Pakhtunkhwa, Pakistan. METHODS: A total of 349 drug-treated and untreated TB-diagnosed patients were enrolled in this study...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28490367/vitamin-d-deficiency-among-smear-positive-pulmonary-tuberculosis-patients-and-their-tuberculosis-negative-household-contacts-in-northwest-ethiopia-a-case-control-study
#7
Belay Tessema, Feleke Moges, Dereje Habte, Nebiyu Hiruy, Shewaye Yismaw, Kassahun Melkieneh, Yewulsew Kassie, Belaineh Girma, Muluken Melese, Pedro G Suarez
BACKGROUND: Vitamin D is a fat-soluble vitamin that increases the immunity against tuberculosis (TB), decreases the re-activation of latent TB and reduces the severity of active TB disease. Epidemiological studies on the prevalence of vitamin D deficiency, and its association with TB showed inconsistent results in different countries. This study was aimed to determine the prevalence of vitamin D deficiency and its association with TB in Northwest Ethiopia. METHODS: A case-control study was conducted among smear positive pulmonary tuberculosis patients and their household contacts without symptoms suggestive of TB...
May 11, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28472124/performance-of-taqman-array-card-to-detect-tb-drug-resistance-on-direct-specimens
#8
Sayera Banu, Suporn Pholwat, Suporn Foongladda, Rattapha Chinli, Duangjai Boonlert, Sara Sabrina Ferdous, S M Mazidur Rahman, Arfatur Rahman, Shahriar Ahmed, Eric R Houpt
Culture based phenotypic drug susceptibility testing (DST) for Mycobacterium tuberculosis (TB) is time consuming therefore rapid genotypic methods are increasingly being utilized. We previously developed and evaluated on TB isolates a rapid genotypic TaqMan array card (TAC) that detects mutations in several resistance-associated genes using dozens of primer pairs, probes, and high resolution melt analysis, with >96% accuracy versus Sanger sequencing. In this study we examined the performance of TAC on sputum, comparing results between 71 paired sputum and TB isolates of which 62 were MDR-TB...
2017: PloS One
https://www.readbyqxmd.com/read/28446574/the-use-of-genexpert-remnants-for-drug-resistance-profiling-and-molecular-epidemiology-of-tuberculosis-in-libreville-gabon
#9
Amel Kévin Alame-Emane, Catherine Pierre-Audigier, Oriane Cordelia Aboumegone-Biyogo, Amandine Nzoghe-Mveang, Véronique Cadet-Daniel, Christophe Sola, Joël Fleury Djoba-Siawaya, Brigitte Gicquel, Howard E Takiff
Multidrug (MDR) and extensively drug resistant (XDR) Mycobacterium tuberculosis are major problems in global health. The GeneXpertMTB/RIF (Xpert) rapidly detects resistance to rifampicin (RIF-R), but detection of the additional resistance that defines MDR and XDR-TB, and for molecular epidemiology, specimen cultures and biosafe infrastructure are generally required. We sought to determine whether the remnants of sputa prepared for Xpert could be used directly to find mutations associated with drug resistance and for molecular epidemiology, and thus provide a precise characterization of MDR-TB cases in countries lacking BSL3 facilities for M...
April 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28438221/isolated-splenic-tuberculosis-with-subsequent-paradoxical-deterioration-a-case-report
#10
Frederick Wangai, Loice Achieng, George Otieno, Jacqueline Njoroge, Tabitha Wambaire, Jamilla Rajab
BACKGROUND: Isolated tuberculosis of the spleen has been described occasionally in literature, mostly in immunosuppressed individuals with various risk factors. Sequestration in the spleen makes such Mycobacterium tuberculosis infection difficult to diagnose. This report describes an extremely rare case of isolated splenic tuberculosis in an immunocompetent individual. CASE PRESENTATION: A 26 year old Kenyan male presented with pyrexia of unknown origin, with negative screening tests for bacterial, fungal and parasitic infections...
April 24, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28410700/genexpert-a-momentous-innovation-that-needs-a-touch-of-prudence
#11
EDITORIAL
Mandira Varma-Basil, Ashok Shah
No abstract text is available yet for this article.
April 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28399817/assessments-of-serum-copper-and-zinc-concentration-and-the-cu-zn-ratio-determination-in-patients-with-multidrug-resistant-pulmonary-tuberculosis-mdr-tb-in-c%C3%A3-te-d-ivoire
#12
Gnogbo Alexis Bahi, Lydie Boyvin, Souleymane Méité, Gervais Melaine M'Boh, Kadjowely Yeo, Kouassi Raymond N'Guessan, Alain Dit Philippe Bidié, Allico Joseph Djaman
BACKGROUND: In Côte d'Ivoire, multidrug-resistant tuberculosis (MDR-TB) is a serious public health problem with a prevalence estimated at 2.5% in 2006. Zinc and copper are essential Trace element needed to strengthen the immune system and also useful in the fight against tuberculosis. The Cu / Zn ratio is a good indicator of oxidative stress. The principal aim of this study was to evaluate the serum concentration of some trace element and determine the Cu / Zn ratio in patients with multidrug resistant pulmonary tuberculosis (MDR-TB) before and after second line treatment of TB...
April 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28362869/high-prevalence-of-curable-sexually-transmitted-infections-among-pregnant-women-in-a-rural-county-hospital-in-kilifi-kenya
#13
Simon Chengo Masha, Elizabeth Wahome, Mario Vaneechoutte, Piet Cools, Tania Crucitti, Eduard J Sanders
BACKGROUND: Women attending antenatal care (ANC) in resource-limited countries are frequently screened for syphilis and HIV, but rarely for other sexually transmitted infections (STIs). We assessed the prevalence of curable STIs, defined as infection with either Chlamydia trachomatis or Neisseria gonorrhoeae or Trichomonas vaginalis, from July to September 2015. METHODS: In a cross-sectional study, women attending ANC at the Kilifi County Hospital, Kenya, had a urine sample tested for C...
2017: PloS One
https://www.readbyqxmd.com/read/28358797/evaluation-of-automated-molecular-testing-rollout-for-tuberculosis-diagnosis-using-routinely-collected-surveillance-data-uganda-2012-2015
#14
Colleen Scott, Simon Walusimbi, Bruce Kirenga, Moses Joloba, Muttamba Winters, Nyombi Abdunoor, Rommel Bain, Heather Alexander, Tom Shinnick, Sean Toney, Rosemary Odeke, Christine Mwangi, Estella Birabwa, Seyoum Dejene, Frank Mugabe, Mukadi YaDiul, J Sean Cavanaugh
In 2012, Uganda introduced the use of GeneXpert MTB/RIF (Cepheid, Sunnyvale CA), a sensitive, automated, real-time polymerase chain reaction-based platform for tuberculosis (TB) diagnosis, for programmatic use among children, adults with presumptive human immunodeficiency virus (HIV)-associated TB, and symptomatic persons at risk for rifampicin (RIF)-resistant TB. The effect of using the platform's Xpert MTB/RIF assay on TB care and control was assessed using routinely collected programmatic data; in addition, a retrospective review of district quarterly summaries using abstracted TB register data from purposively selected facilities in the capital city of Kampala was conducted...
March 31, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28351283/evaluation-of-genexpert-mtb-rif-for-detecting-mycobacterium-tuberculosis-in-a-hospital-in-china
#15
Tingyu Tang, Fang Liu, Xiaoling Lu, Qingdong Huang
Objective To evaluate the performance of GeneXpert MTB/RIF in diagnosing pulmonary tuberculosis (TB) in China. Methods This cross-sectional study included sputum specimens of 240 suspected TB cases. Specimens were examined by light microscopy for the presence of acid-fast bacilli, which were cultured by the BACTEC MGIT 960 (M960) system and detected by the GeneXpert MTB/RIF assay. The positive rate, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and average turnaround time of methods were evaluated...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28321579/performance-of-three-generations-of-xpert-mrsa-in-routine-practice-approaching-the-aim
#16
H Jacqmin, A Schuermans, S Desmet, J Verhaegen, V Saegeman
The aim of this study was to evaluate retrospectively the performance of the Xpert MRSA assay in routine practice and its current use in the intensive care unit (ICU) setting of our hospital, since a pre-emptive isolation strategy has been applied. A total of 6473 patients were routinely screened with ESwab for methicillin-resistant Staphylococcus aureus (MRSA) using three generations of rapid real-time polymerase chain reaction (PCR) (Cepheid GeneXpert) over three consecutive periods of time. Performance was evaluated using broth enrichment culture as the reference method...
March 20, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28317800/are-who-approved-nucleic-acid-amplification-tests-causing-large-scale-false-identification-of-rifampicin-resistant-tuberculosis-programmatic-experience-from-south-india
#17
Praveen Sanker, Anusree P Ambika, Vishnu T Santhosh, Ramya Puthukkudi Kottuthodi, Ravikrishnan Balakrishnan, Sunil Kumar Mrithunjayan, Hisham Moosan
INTRODUCTION: The nucleic acid amplification tests (NAATs): Line probe assay and GeneXpert (Xpert) have evolved as the primary tool for identification of rifampicin (RIF)-resistant (RR) tuberculosis (TB) worldwide, primarily because of the ease and speed. We rechecked RR isolates identified by NAATs from presumptive RR TB cases belonging to South India by the Revised National TB Control Program, India using multiple RIF concentrations on Bactec MGIT system and compared the mutation patterns with the resistance levels...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28278193/a-bcr-abl1-cutoff-of-1-5-at-3-months-determined-by-the-genexpert-system-predicts-an-optimal-response-in-patients-with-chronic-myeloid-leukemia
#18
Valentín García-Gutiérrez, María T Gómez-Casares, José M Puerta, Juan M Alonso-Domínguez, Santiago Osorio, Juan C Hernández-Boluda, Rosa Collado, María J Ramírez, Fátima Ibáñez, María L Martín, Juan D Rodríguez-Gambarte, Carolina Martínez-Laperche, Montse Gómez, Dolly V Fiallo, Sara Redondo, Alicia Rodríguez, Concepción Ruiz-Nuño, Juan L Steegmann, Antonio Jiménez-Velasco
In chronic myeloid leukemia (CML) patients, 3-month BCR-ABL1 levels have consistently been correlated with further outcomes. Monitoring molecular responses in CML using the GeneXpert (Cepheid) platform has shown an optimal correlation with standardized RQ-PCR (IS) when measuring BCR-ABL1 levels lower than 10%, as it is not accurate for values over 10%. The aim of the present study was to determine the predictive molecular value at three months on different outcome variables using the Xpert BCR-ABL1 MonitorTM assay (Xpert BCR-ABL1)...
2017: PloS One
https://www.readbyqxmd.com/read/28258004/evaluation-of-genexpert-mtb-rif-for-detection-of-pulmonary-tuberculosis-at-peripheral-tuberculosis-clinics
#19
Yan Shao, Hong Peng, Cheng Chen, Tao Zhu, Ming Ji, Wei Jiang, Wei Zhu, Xiang Jun Zhai, Wei Lu
Tuberculosis is one of the most common infectious diseases in China, while delayed patient finding obstructed disease control, especially for smear-negative patients. The current study was undertaken to evaluate the diagnostic accuracy of GeneXpert MTB/RIF compared with conventional methods in the detection of pulmonary tuberculosis patients. A total of 295 spot sputum samples from confirmed pulmonary tuberculosis patients were evaluated from September 2014 to June 2015. Each sample was examined by acid-fast bacillus smear microscopy, culture and GeneXpert MTB/RIF...
April 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28247256/-rare-cutaneous-tuberculosis-in-sub-saharan-africa-developed-on-discoid-lupus-erythematous-lesion
#20
A Diop, M T Ndiaye, M Ndiaye, B Seck, A Diouf, B H Diatta, M Diallo, F Ly
Lupus vulgaris is a common presentation of cutaneous tuberculosis (TB), but its ulcerative or vegetating form also called vorax is rare.We report a case of lupus vulgaris in its vorax form, which occurred in a patient with discoid lupus erythematous. A 42-year-old patient monitored for chronic lupus erythematosus consulted again for a facial tumor and fever. Physical examination revealed painful ulcerative and crusted lesions on an old discoid lupus lesion and covered the entire upper lip. Similar lesions were noted on cheeks...
February 28, 2017: Bulletin de la Société de Pathologie Exotique
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