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https://www.readbyqxmd.com/read/28534454/opportunities-and-challenges-in-metabarcoding-approaches-for-helminth-community-identification-in-wild-mammals
#1
Tuomas Aivelo, Alan Medlar
Despite metabarcoding being widely used to analyse bacterial community composition, its application in parasitological research remains limited. What interest there has been has focused on previously intractable research settings where traditional methods are inappropriate, for example, in longitudinal studies and studies involving endangered species. In settings such as these, non-invasive sampling combined with metabarcoding can provide a fast and accurate assessment of component communities. In this paper we review the use of metabarcoding in the study of helminth communities in wild mammals, outlining the necessary procedures from sample collection to statistical analysis...
May 23, 2017: Parasitology
https://www.readbyqxmd.com/read/28527688/clinical-development-of-a-var2csa-based-placental-malaria-vaccine-pamvac-quantifying-vaccine-antigen-specific-memory-b-t-cell-activity-in-beninese-primigravidae
#2
Komi Gbédandé, Nadine Fievet, Firmine Viwami, Sem Ezinmegnon, Saadou Issifou, Jean-Philippe Chippaux, Yannelle Dossou, Kabirou Moutairou, Achille Massougbodji, Nicaise Ndam, Willem Adriaan de Jongh, T Max M Søgaard, Ali Salanti, Morten A Nielsen, Meral Esen, Benjamin Mordmüller, Philippe Deloron, Adrian J F Luty
BACKGROUND: The antigen VAR2CSA plays a pivotal role in the pathophysiology of pregnancy-associated malaria (PAM) caused by Plasmodium falciparum. A VAR2CSA-based vaccine candidate, PAMVAC, is under development by an EU-funded multi-country consortium (PlacMalVac project). As part of PAMVAC's clinical development, we quantified naturally acquired vaccine antigen-specific memory B and T cell responses in Beninese primigravidae recruited at the beginning of pregnancy and followed up to delivery and beyond...
May 17, 2017: Vaccine
https://www.readbyqxmd.com/read/28526088/assessment-of-parasitological-findings-in-heartworm-infected-beagles-treated-with-advantage-multi%C3%A2-for-dogs-10-imidacloprid%C3%A2-%C3%A2-2-5-moxidectin-and-doxycycline
#3
Molly D Savadelis, Cameon M Ohmes, Joe A Hostetler, Terry L Settje, Robert Zolynas, Michael T Dzimianski, Andrew R Moorhead
BACKGROUND: Anecdotal reports support the position that the adulticidal heartworm treatment utilizing doxycycline and Advantage Multi®/Advocate® for Dogs (10% imidacloprid + 2.5% moxidectin) has successfully converted antigen-positive dogs to antigen-negative. To date, no controlled experimental studies have demonstrated the adulticidal efficacy of this treatment regimen. The aim of this study was to evaluate the parasitological and clinical efficacy of Advantage Multi® for Dogs (IMD + MOX) and doxycycline in heartworm-infected beagles...
May 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28521798/use-of-malaria-rdts-in-various-health-contexts-across-sub-saharan-africa-a-systematic-review
#4
Matthew R Boyce, Wendy P O'Meara
BACKGROUND: The World Health Organization recommends parasitological confirmation of malaria prior to treatment. Malaria rapid diagnostic tests (RDTs) represent one diagnostic method that is used in a variety of contexts to overcome limitations of other diagnostic techniques. Malaria RDTs increase the availability and feasibility of accurate diagnosis and may result in improved quality of care. Though RDTs are used in a variety of contexts, no studies have compared how well or effectively RDTs are used across these contexts...
May 18, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28516228/great-sandeel-hyperoplus-lanceolatus-as-a-putative-transmitter-of-parasite-contracaecum-osculatum-nematoda-anisakidae
#5
K Nadolna-Ałtyn, M Podolska, B Szostakowska
In the Baltic Sea, the great sandeel (Hyperoplus lanceolatus), a common fish species that inhabits sandy substrates, is an important element in the diet of marine mammals, sea birds, and piscivorous fish. It can also act as a transmitter of parasites to its predators, but parasitological studies on this species are limited. We sampled great sandeels in April 2016 in two areas of the southern Baltic Sea (SB-south of Bornholm and GG-the Gulf of Gdansk) and found parasitic anisakid nematodes in both locations...
May 17, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28510359/isolation-culture-and-cryopreservation-of-sarcocystis-species
#6
S K Verma, D S Lindsay, M E Grigg, J P Dubey
More than 200 valid Sarcocystis species have been described in the parasitological literature. The developmental life cycle in the intermediate host and definitive host has only been described for a few species. Sarcocystis parasites are common pathogens infecting a wide range of animals, including humans, and this unit reviews the methods used for isolating infective stages of the parasite from both definitive and intermediate host(s), as well as methods used to initiate cultures from sporocysts and merozoites and for cryopreservation of various Sarcocystis spp...
May 16, 2017: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/28501468/-tinea-capitis-main-mycosis-child-epidemiological-study-on-10years
#7
A Kallel, A Hdider, N Fakhfakh, S Belhadj, N Belhadj-Salah, N Bada, A Chouchen, S Ennigrou, K Kallel
Despite the changes in their epidemiology, and the improving level of hygiene of the population, tinea capitis is still considered a public health problem in our country, and is the most common type of dermatophytosis in our country. The aim of our study was to evaluate the epidemiological, clinical and mycological features of tinea capitis in children encountered in the Tunis region. A retrospective study concerned 1600 children aged 6 months to 15 years suspected to have tinea capitis was conducted in Parasitology-Mycology laboratory, Rabta hospital, over a 10-years period (2005-2014)...
May 10, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28499435/parasite-fauna-of-the-antarctic-dragonfish-parachaenichthys-charcoti-perciformes-bathydraconidae-and-closely-related-bathydraconidae-from-the-antarctic-peninsula-southern-ocean
#8
Julian Münster, Judith Kochmann, Juline Grigat, Sven Klimpel, Thomas Kuhn
BACKGROUND: As members of the Notothenioidei - the dominant fish taxon in Antarctic waters - the family Bathydraconidae includes 12 genera and 17 species. The knowledge of these species inhabiting an isolated environment is rather fragmentary, including their parasite fauna. Studies on fish hosts and their associated parasites can help gain insights into even remote ecosystems and be used to infer ecological roles in food webs; however, ecological studies on the Bathydraconidae are scarce...
May 12, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28497029/reciprocal-interactions-between-nematodes-and-their-microbial-environments
#9
REVIEW
Ankur Midha, Josephine Schlosser, Susanne Hartmann
Parasitic nematode infections are widespread in nature, affecting humans as well as wild, companion, and livestock animals. Most parasitic nematodes inhabit the intestines of their hosts living in close contact with the intestinal microbiota. Many species also have tissue migratory life stages in the absence of severe systemic inflammation of the host. Despite the close coexistence of helminths with numerous microbes, little is known concerning these interactions. While the environmental niche is considerably different, the free-living nematode Caenorhabditis elegans (C...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28491315/subcutaneous-dirofilariasis-caused-by-dirofilaria-nochtiella-repens-in-sri-lanka-a-potential-risk-of-transmitting-human-dirofilariasis
#10
Nayana Gunathilaka, Shirom Siriwardana, Lakmini Wijesooriya, Gayana Gunaratne, Nilanga Perera
OBJECTIVE: Dirofilariasis is a parasitic infection caused by filarial nematodes belonging to the genus Dirofilaria. Human dirofilariasis due to Dirofilaria repens has been widely reported from European countries. Sri Lanka is the most affected country in Asia with an infection rate of almost 60% in dog population. However, human infection of D. repens remains undiagnosed and many of the cases are not scientifically documented. Therefore, the objective of the present investigation is to document the presence of dirofilariasis infections in humans...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28486259/appendicitis-in-a-neutropenic-patient-a-multicentric-retrospective-study
#11
Alberto A Scarpa, Géraldine Hery, Arnaud Petit, Benoit Brethon, Irène Jimenez, Virginie Gandemer, Samuel Abbou, Stéphanie Haouy, Jean Breaud, Marilyne Poiree
To study the management of acute appendicitis in neutropenic patients, we retrospectively reviewed cases of acute appendicitis in neutropenic children treated for cancer. The patients' demographics, medical records, and outcomes were tracked. We compared nonoperative treatment versus emergency or delayed surgery. The cases of 30 patients with a mean age of 8.8 years in 12 French departments of Pediatric Hematology/Oncology between 1995 and 2013 were studied. Most patients (90%) were treated for hematological malignancies...
May 8, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28484348/the-most-frequent-isolates-from-outpatients-with-urinary-tract-infection
#12
Sabina Mahmutovic Vranic, Nadira Zatric, Velma Rebic, Mufida Aljicevic, Amila Abdulzaimovic
OBJECTIVES: The main objective of the study was to identify the most frequent isolates from female outpatients with urinary tract infection. MATERIAL AND METHODS: The retrospective study was performed at the Department of Microbiology, Parasitology and Virology Faculty of Medicine, University of Sarajevo in period of 2015. The most frequent isolates were determined in 405 first urine samples from female outpatients of Hrasno community in Sarajevo, B&H. Identification of isolates was performed by the standard microbiological testing and the standard methods of descriptive statistics as well...
March 2017: Materia Socio-medica
https://www.readbyqxmd.com/read/28483733/the-trematode-parasites-of-lophius-piscatorius-angler-fish-from-the-aegean-sea
#13
Yahya Tepe
OBJECTIVE: There is no study on the trematode parasites of Lophius piscatorius. The aim of this study is to address the lack of knowledge about the parasites of angler fish from the coasts of Turkish seas. METHODS: Frozen individuals of L. piscatorius from the coasts of Izmir were brought to Ataturk University, and their visceral organs were parasitologically investigated. Parasites were fixed with AFA (Acetic acid-Formaline-Alcohol) fixative and permanently mounted with Canada balsam...
March 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/28483729/presence-of-parasites-in-environmental-waters-in-samsun-and-its-districts
#14
Ülkü Karaman, Zeynep Kolören, Onuralp Seferoğlu, Emine Ayaz, Elif Demirel
OBJECTIVE: The aim of this study was to detect the presence of parasites in environmental waters in Samsun and its districts. METHODS: At the center of Samsun, 13 stations were determined. The research was performed between March 2012 and February 2013, and every month, water samples were collected on the dates stated. The samples were stained with Kinyoun acid-fast, modified trichrome, and trichrome dyes after examining with the direct bond. The preparations were evaluated in terms of parasitologic under a light microscope...
March 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/28483382/modeling-key-drivers-of-cholera-transmission-dynamics-provides-new-perspectives-for-parasitology
#15
REVIEW
Andrea Rinaldo, Enrico Bertuzzo, Melanie Blokesch, Lorenzo Mari, Marino Gatto
Hydroclimatological and anthropogenic factors are key drivers of waterborne disease transmission. Information on human settlements and host mobility on waterways along which pathogens and hosts disperse, and relevant hydroclimatological processes, can be acquired remotely and included in spatially explicit mathematical models of disease transmission. In the case of epidemic cholera, such models allowed the description of complex disease patterns and provided insight into the course of ongoing epidemics. The inclusion of spatial information in models of disease transmission can aid in emergency management and the assessment of alternative interventions...
May 5, 2017: Trends in Parasitology
https://www.readbyqxmd.com/read/28473912/high-burden-of-carbapenem-resistant-enterobacteriaceae-cre-fecal-carriage-at-a-teaching-hospital-cost-effectiveness-of-screening-in-low-resource-setting
#16
Abdul Rahman Zaidah, Nurul Izzah Mohammad, Siti Suraiya, Azian Harun
BACKGROUND: Infections by multidrug-resistant gram-negative bacteria (MDR-GNB) have been continuously growing and pose challenge to health institution globally. Carbapenem-resistant Enterobacteriacea (CRE) was identified as one of the MDR-GNB which has limited treatment options and higher mortality compared to those of sensitive strains. We report an increased burden of CRE fecal carriage at a hospital in the North-eastern region of Malaysia. METHODS: A retrospective descriptive study from August 2013 to December 2015 was conducted in the Medical Microbiology & Parasitology laboratory of Hospital Universiti Sains Malaysia, which is a tertiary teaching hospital with more than 700 beds...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28473241/biology-of-parasitism-a-broad-and-deep-parasitology-discovery-course
#17
(no author information available yet)
No abstract text is available yet for this article.
May 1, 2017: Trends in Parasitology
https://www.readbyqxmd.com/read/28470015/econsultations-to-infectious-disease-specialists-questions-asked-and-impact-on-primary-care-providers-behavior
#18
Ruchi Murthy, Gregory Rose, Clare Liddy, Amir Afkham, Erin Keely
BACKGROUND: Since 2010, the Champlain BASE (Building Access to Specialist Advice through eConsultation) has allowed primary care providers (PCPs) to submit clinical questions to specialists through a secure web service. The study objectives are to describe questions asked to Infectious Diseases specialists through eConsultation and assess impact on physician behaviors. METHODS: eConsults completed through the Champlain BASE service from April 15, 2013 to January 29, 2015 were characterized by the type of question asked and infectious disease content...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28466247/is-trace-element-concentration-correlated-to-parasite-abundance-a-case-study-in-a-population-of-the-green-frog-pelophylax-synkl-hispanicus-from-the-neto-river-calabria-southern-italy
#19
Carlo De Donato, Donatella Barca, Concetta Milazzo, Raffaella Santoro, Gianni Giglio, Sandro Tripepi, Emilio Sperone
Bioaccumulation of 13 trace elements in the livers of 38 Pelophylax sinkl. hispanicus (Ranidae) and its helminth communities were studied and compared among three sites, each with a different degree of pollution along River Neto (south Italy) during September, 2014. Trace element concentrations in water and liver were measured using inductively coupled plasma mass spectrometry. For most elements, the highest concentration was recorded in the frogs inhabiting the third site, the one with the highest degree of pollution...
May 2, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28465550/in-vivo-efficacy-of-chloroquine-to-clear-asymptomatic-infections-in-mozambican-adults-a-randomized-placebo-controlled-trial-with-implications-for-elimination-strategies
#20
Beatriz Galatas, Lidia Nhamussua, Baltazar Candrinho, Lurdes Mabote, Pau Cisteró, Himanshu Gupta, Regina Rabinovich, Clara Menéndez, Eusebio Macete, Francisco Saute, Alfredo Mayor, Pedro Alonso, Quique Bassat, Pedro Aide
Recent reports regarding the re-emergence of parasite sensitivity to chloroquine call for a new consideration of this drug as an interesting complementary tool in malaria elimination efforts, given its good safety profile and long half-life. A randomized (2:1), single-blind, placebo-controlled trial was conducted in Manhiça, Mozambique, to assess the in-vivo efficacy of chloroquine to clear plasmodium falciparum (Pf) asymptomatic infections. Primary study endpoint was the rate of adequate and parasitological response (ACPR) to therapy on day 28 (PCR-corrected)...
May 2, 2017: Scientific Reports
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