keyword
MENU ▼
Read by QxMD icon Read
search

adamts

keyword
https://www.readbyqxmd.com/read/28810627/carthamin-yellow-inhibits-matrix-degradation-and-inflammation-induced-by-lps-in-the-intervertebral-disc-via-suppression-of-mapk-pathway-activation
#1
Bin Chen, Han-Tao Wang, Bo Yu, Ji-Dong Zhang, Yu Feng
Carthamin yellow (CY), which is a flavonoid compound isolated from safflower, has various pharmacological effects including promoting blood circulation to remove blood stasis and alleviating pain. CY is a herb used in Chinese traditional medicines. Intervertebral disc degeneration (IDD) is a common spinal disorder and degeneration of nucleus pulposus (NP) cells and inflammation are significant parts of the pathological cascade. The curative effect of CY on NP cells in association with degeneration and inflammation remains to be elucidated...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28810568/mechanism-of-microrna-146a-mediated-il-6-stat3-signaling-in-lumbar-intervertebral-disc-degeneration
#2
Tao Zhou, Hao Lin, Ziao Cheng, Chaochao Ji, Chao Zhang, Jiwei Tian
The aim of the study was to investigate the mechanism of microRNA (miR)-146a-mediated activation of interleukin-6/signal transducer and activator of transcription 3 (IL-6/STAT3) in lumbar intervertebral disc degeneration. To obtain intervertebral tissue, we recruited 5 patients with lumbar intervertebral disc herniation (experimental group) and 5 patients with lumbar burst fracture (control group). Nucleus pulposus tissue was extracted by surgery and cultured. miR-146a empty vector, mimic, and inhibitor were transfected into the two groups of cells for 24 h and the levels of IL-6, type II collagen (Col II), aggrecan, STAT3, matrix metalloproteinase (MMP)-3, and a disintegrin and metalloproteinase with thrombospondin type I motifs (ADAMTS) were detected...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28807411/timp3-is-regulated-by-pericytes-upon-shear-stress-detection-leading-to-a-modified-endothelial-cell-response
#3
C Schrimpf, T Koppen, J S Duffield, U Böer, S David, W Ziegler, A Haverich, O E Teebken, M Wilhelmi
OBJECTIVES: Atherosclerosis is a hallmark of cardiovascular disease. Shear stress on endothelial cells has been linked to atherogenesis and to fibrous cap thinning and rupture. Pericytes reside in the sub-endothelial space of vessels and have vasoprotective effects. They are subjected to shear stress when endothelial cell integrity is disrupted. The aim was to investigate the susceptibility and response of pericytes to shear stress. METHODS: Endothelial cells and pericytes were seeded in two dimensional monocultures and co-cultures, and in a novel three dimensional co-culture system and were subjected to no, low and high shear stress (0, 10, 30 dyne/cm(2)) for 48 h...
August 11, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28798097/suramin-inhibits-osteoarthritic-cartilage-degradation-by-increasing-extracellular-levels-of-chondroprotective-tissue-inhibitor-of-metalloproteinases-3-timp-3
#4
Anastasios Chanalaris, Christine Doherty, Brian D Marsden, Gabriel Bambridge, Stephen P Wren, Hideaki Nagase, Linda Troeberg
Osteoarthritis is a common degenerative joint disease for which no disease-modifying drugs are currently available. Attempts to treat the disease with small molecule inhibitors of the metalloproteinases that degrade the cartilage matrix have been hampered by a lack of specificity. We aimed to inhibit cartilage degradation by augmenting levels of the endogenous metalloproteinase inhibitor, tissue inhibitor of metalloproteinases 3 (TIMP-3), through blocking its interaction with the endocytic scavenger receptor, low-density lipoprotein receptor-related protein 1 (LRP1)...
August 10, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28775254/adamts-13-regulates-neutrophil-recruitment-in-a-mouse-model-of-invasive-pulmonary-aspergillosis
#5
Astrid Alflen, Steve Prüfer, Katharina Ebner, Sebastian Reuter, Pamela Aranda Lopez, Inge Scharrer, Fumiaki Banno, Michael Stassen, Hansjörg Schild, Kerstin Jurk, Markus Bosmann, Hendrik Beckert, Markus P Radsak
Von Willebrand factor (VWF) is secreted as an acute phase protein during inflammation. ADAMTS-13 regulates the size and prothrombotic activity of VWF by it's specific proteolytic activity. To determine the relevance of this regulatory pathway for the innate inflammatory response by polymorphonuclear neutrophils (PMN), we employed a mouse model of invasive pulmonary aspergillosis (IPA) where PMN functionality is crucial for fungal clearance and survival. IPA was induced by intratracheal application of Aspergillus fumigatus (A...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28766346/dose-dependent-effects-of-adiponectin-on-adamts-9-gene-expression-in-human-chondrocytes
#6
K O Yaykasli, O F Hatipoglu, E Yaykasli, E Kaya, M Ozsahin, M Uslu
OBJECTIVE: A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), comprising of 19 members is a family of peptidases. They have several vital functions in physiological and pathological processes in organisms. ADAMTS-9 has aggrecanolytic activity and is responsible for degradation of aggrecan mainly in articular cartilage. It is known that adiponectin is the most abundantly secreted adipokine (adipocytokines), and the characteristics of adiponectin have not been elucidated yet...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/28765635/conditional-knockdown-of-hyaluronidase-2-in-articular-cartilage-stimulates-osteoarthritic-progression-in-a-mice-model
#7
Yoshitoshi Higuchi, Yoshihiro Nishida, Eiji Kozawa, Lisheng Zhuo, Eisuke Arai, Shunsuke Hamada, Daigo Morita, Kunihiro Ikuta, Koji Kimata, Takahiro Ushida, Naoki Ishiguro
The catabolism of hyaluronan in articular cartilage remains unclear. The aims of this study were to investigate the effects of hyaluronidase 2 (Hyal2) knockdown in articular cartilage on the development of osteoarthritis (OA) using genetic manipulated mice. Destabilization of the medial meniscus (DMM) model of Col2a promoter specific conditional Hyal2 knockout (Hyal (-/-) ) mice was established and examined. Age related and DMM induced alterations of articular cartilage of knee joint were evaluated with modified Mankin score and immunohistochemical staining of MMP-13, ADAMTS-5, KIAA11199, and biotinylated- hyaluronan binding protein staining in addition to histomorphometrical analyses...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28761774/proinflammatory-and-anabolic-gene-expression-effects-of-platelet-rich-gel-supernatants-on-equine-synovial-membrane-explants-challenged-with-lipopolysaccharide
#8
Jorge U Carmona, Diana L Ríos, Catalina López, María E Álvarez, Jorge E Pérez
Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS)...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/28760813/the-extracellular-metalloprotease-adamts-a-anchors-neural-lineages-in-place-within-and-preserves-the-architecture-of-the-central-nervous-system
#9
James B Skeath, Beth A Wilson, Selena E Romero, Mark J Snee, Yi Zhu, Haluk Lacin
The extracellular matrix (ECM) regulates cell migration and sculpts organ shape. AdamTS proteins are extracellular metalloproteases known to modify ECM proteins and promote cell migration, but demonstrated roles for AdamTS proteins in regulating CNS structure and ensuring cell lineages remain fixed in place have not been uncovered. Using forward genetic approaches in Drosophila, we find that reduction of AdamTS-A function induces both the mass exodus of neural lineages out of the CNS and drastic perturbations to CNS structure...
July 31, 2017: Development
https://www.readbyqxmd.com/read/28752178/inhibitory-effect-of-jak-inhibitor-on-mechanical-stress-induced-protease-expression-by-human-articular-chondrocytes
#10
Takahiro Machida, Keiichiro Nishida, Yoshihisa Nasu, Ryuichi Nakahara, Masatsugu Ozawa, Ryozo Harada, Masahiro Horita, Ayumu Takeshita, Daisuke Kaneda, Aki Yoshida, Toshifumi Ozaki
OBJECTIVE: To investigate whether janus kinase (JAK) inhibitor exhibits a chondro-protective effect against mechanical stress-induced expression of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) and matrix metalloproteinase (MMPs) in human chondrocytes. MATERIALS AND METHODS: Normal human articular chondrocytes were seeded onto stretch chambers and incubated with or without tofacitinib (1000 nM) for 12 h before mechanical stimulation or cytokine stimulation...
July 27, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28744817/knockdown-of-prmt1-suppresses-il-1%C3%AE-induced-cartilage-degradation-and-inflammatory-responses-in-human-chondrocytes-through-gli1-mediated-hedgehog-signaling-pathway
#11
Lei Xia, Hong-Xing Zhang, Mei-Li Xing, Yu-Ben Xu, Peng Li, Liang-Ku Huang, Jie Bai, Zhao Tian, Zan-Dong Zhao
Osteoarthritis (OA) is characterized by articular cartilage degradation and joint inflammation. The purpose of the present study is to elucidate the role of the specific function of PRMT1 in chondrocytes and its association with the pathophysiology of OA. We observed that the expression of PRMT1 was apparently upregulated in OA cartilage, as well as in chondrocytes stimulated with IL-1β. Additionally, knockdown of PRMT1 suppressed interleukin 1 beta (IL-1β)-induced extracellular matrix (ECM) metabolic imbalance by regulating the expression of MMP-13, ADAMTS-5, COL2A1, and ACAN...
July 25, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28744016/anti-inflammatory-and-chondroprotective-effects-of-the-s-adenosylhomocysteine-hydrolase-inhibitor-3-deazaneplanocin-a-in-human-articular-chondrocytes
#12
Juliette Aury-Landas, Céline Bazille, Lyess Allas, Sara Bouhout, Christophe Chesneau, Sylvain Leclercq, Karim Boumédiene, Catherine Baugé
3-Deazaneplanocin A (DZNep) is an inhibitor of S-Adenosyl-L-Homocysteine Hydrolase (SAHH) known to inhibit EZH2, a histone methylase upregulated during osteoarthritis. In this study, we assessed its effects in human articular chondrocytes. Anti-inflammatory effects were assessed by Nitric Oxide (NO), Prostaglandin E2 (PGE2) and Metalloprotease (MMP) release in IL-1β-stimulated chondrocytes. MAPK and NFκB activation was analyzed by western blotting. Differentially expressed genes (DEG) regulated by DZNep were identified by whole-transcriptome microarray...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743060/glucose-fluctuation-and-the-resultant-endothelial-injury-are-correlated-with-pancreatic-%C3%AE-cell-dysfunction-in-patients-with-coronary-artery-disease
#13
Makoto Murata, Hitoshi Adachi, Shigeru Oshima, Masahiko Kurabayashi
AIMS: We evaluated whether glucose fluctuation (GF) causes vascular endothelial injury and affects glucometabolic factors during lengthy oral glucose tolerance test (OGTT). METHODS: We enrolled consecutive 116 patients with coronary artery disease (CAD) who were performed coronary angiography and 4-h OGTT. Blood samples were collected before and 4h after glucose load to measure endothelial injury factor [von Willebrand factor (vWF) and vWF/a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13 (ADAMTS-13) ratio]...
July 8, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/28736153/in-vitro-study-of-gonadotrophin-signaling-pathways-in-human-granulosa-cells-in-relation-to-progesterone-receptor-expression
#14
Soledad Henríquez, Paulina Kohen, Alex Muñoz, Ana Godoy, Felipe Orge, Jerome F Strauss, Luigi Devoto
In humans, data on gonadotrophin-activated (LH, HCG and FSH) progesterone receptor expression and signalling pathways involved in matrix metalloproteinases (MMPs) expression presumably linked to the follicle rupture, are limited. Our hypothesis is LH, HCG and FSH increase progesterone receptor expression in granulosa cells through different signalling pathways, leading to an increased expression of ADAMTS-1 and MMP3/10, which may mediate follicular rupture through the transcription factor, HIF1A. Human granulosa cells were isolated from follicular aspirates obtained from 22 healthy women participating in our IVF programme for male-factor infertility...
June 23, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28724754/relevance-of-the-proteolytic-processing-of-reelin-by-adamts-3-in-brain-functions
#15
Sighild Lemarchant
No abstract text is available yet for this article.
July 19, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28724217/chondroprotective-and-anti-nociceptive-effects-of-caffeoylquinic-acid-in-osteoarthritis-by-downregulating-catabolic-activity-and-oxidative-damage-in-chondrocytes
#16
Luping Liu, Yongmei Li
We evaluated the chondroprotective and anti-nociceptive effects of caffeoylquinic acid (CA) in a rat osteoarthritis (OA) model. Monosodium iodoacetate was injected intra-articularly in the rats to produce OA. The rats were divided into three groups: control, OA group, which received vehicle, and the CA-treated group, which received CA (50mg/kg, p.o.) for 14days. At the end of the protocol, pain was estimated by observing the paw withdrawal threshold and latency. Degeneration of cartilage was assessed through histomorphological and microscopical examination of joints...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28682993/quantification-of-von-willebrand-factor-cleavage-by-adamts-13-in-patients-supported-by-left-ventricular-assist-devices
#17
Yong Zhou, Shizhen Qin, Tristan Hilton, Li Tang, Miguel Cruz, Ruben Hernandez, Joel L Moake, Qiang Tian, O H Frazier, Jing-Fei Dong, Angelo Nascimbene
Patients supported by left ventricular assist devices (LVADs) often present with the loss of large von Willebrand factor (VWF) multimers. This VWF deficiency is believed to contribute to the bleeding diathesis of patients on LVAD support and is caused by excessive VWF cleavage by the metalloprotease ADAMTS-13 under high shear stress. However, only a small percentage of patients who have suffered the loss of large VWF multimers bleed. The actual rates of VWF cleavage in these patients have not been reported, primarily because of the lack of reliable detection methods...
July 1, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28677772/vaspin-prevents-leptin%C3%A2-induced-inflammation-and-catabolism-by-inhibiting-the-activation-of-nuclear-factor%C3%A2-%C3%AE%C2%BAb-in-rat-chondrocytes
#18
Jia-Peng Bao, Lang-Hai Xu, Ji-Sheng Ran, Yan Xiong, Li-Dong Wu
The present study aimed to investigate the function and possible underlying mechanism of various concentrations of visceral adipose tissue‑derived serine protease inhibitor (vaspin) on leptin‑induced inflammatory and metabolic responses in rat chondrocytes. Rat articular chondrocytes were isolated and treated with different concentrations of vaspin, which was followed by stimulation with leptin. The expression of genes, secretion of nitric oxide and tumor necrosis factor‑α, and activation of the nuclear factor (NF)‑κB pathway were analyzed by reverse transcription‑quantitative polymerase chain reaction, ELISA and western blotting...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28674292/diverse-functions-of-a-disintegrin-and-metalloproteinase-with-thrombospondin-motif-1
#19
Satoshi Hirohata, Junko Inagaki, Takashi Ohtsuki
 A disintegrin and metalloproteinase with thrombospondin motif-1 (ADAMTS1) was initially cloned from a colon cachexia cell line. In the last 20 years, novel matrix metalloproteinase (MMP) genes were found, and in addition to their original members (MMPs and membrane-type MMPs), the current MMP family contains a disintegrin and metalloproteinases (ADAMs) and ADAMTS. ADAM and ADAMTS play essential roles in organogenesis as well as various diseases including osteoarthritis. ADAMTS has 19 members and can be divided into several groups according to their substrates...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28668088/transforming-growth-factor-activating-kinase-1-regulates-extracellular-matrix-degrading-enzymes-and-pain-related-molecule-expression-following-tumor-necrosis-factor-%C3%AE-stimulation-of-synovial-cells-an-in-vitro-study
#20
Kentaro Uchida, Shotaro Takano, Toshihide Matsumoto, Naoshige Nagura, Gen Inoue, Makoto Itakura, Masayuki Miyagi, Jun Aikawa, Dai Iwase, Atsushi Minatani, Hisako Fujimaki, Masashi Takaso
BACKGROUND: Recent studies have suggested that the tumor necrosis factor-α (TNF-α) pathway is a potential target for the management of osteoarthritis (OA). Transforming growth factor (TGF)-β-activated kinase 1 (TAK1) is essential in several cytokine-mediated cascades, including the TNF-α, interleukin-1 (IL-1), and TGF-β pathways. The role of TAK1 in synovial tissue in OA is not fully understood. Using synovial cells harvested from OA patients during surgery, we investigated whether TAK1 inhibition suppresses production of TNF-α-induced extracellular matrix degrading enzymes and expression of pain-related molecules...
July 1, 2017: BMC Musculoskeletal Disorders
keyword
keyword
53834
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"