keyword
MENU ▼
Read by QxMD icon Read
search

receptors lepidoptera bacillus thuringiensis

keyword
https://www.readbyqxmd.com/read/27456115/three-toxins-two-receptors-one-mechanism-mode-of-action-of-cry1a-toxins-from-bacillus-thuringiensis-in-heliothis-virescens
#1
Anne Bretschneider, David G Heckel, Yannick Pauchet
Insecticidal crystal (Cry) proteins from Bacillus thuringiensis (Bt) are highly active against Lepidoptera. However, field-evolved resistance to Bt toxins is on the rise. The 12-cadherin domain protein HevCaLP and the ABC transporter HevABCC2 are both genetically linked to Cry toxin resistance in Heliothis virescens. We investigated their interaction using stably expressing non-lytic clonal Sf9 cell lines expressing either protein or both together. Untransfected Sf9 cells are innately sensitive to Cry1Ca toxin, but not to Cry1A toxins; and quantitative PCR revealed negligible expression of genes involved in Cry1A toxicity such as cadherin, ABCC2, alkaline phosphatase (ALP) and aminopeptidase N (APN)...
September 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27436734/bacillus-thuringiensis-toxin-resistance-mechanisms-among-lepidoptera-progress-on-genomic-approaches-to-uncover-causal-mutations-in-the-european-corn-borer-ostrinia-nubilalis
#2
REVIEW
Brad S Coates
Transgenic plants that express Bacillus thuringiensis (Bt) crystal (Cry) protein toxins (Bt crops) effectively control feeding by the European corn borer, Ostrinia nubilalis, although documented resistance evolution among a number of species in both the laboratory and field has heightened concerns about the durability of this technology. Research has provided major insights into the mutations that alter Bt toxin binding receptor structure and function within the midgut of Lepidoptera that directly impacts the efficacy of Bt toxins, and potentially leads to the evolution of resistance to Bt crops in the field...
June 2016: Current Opinion in Insect Science
https://www.readbyqxmd.com/read/27333791/sequencing-de-novo-assembly-and-annotation-of-a-pink-bollworm-larval-midgut-transcriptome
#3
Erica E Tassone, Gina Zastrow-Hayes, John Mathis, Mark E Nelson, Gusui Wu, J Lindsey Flexner, Yves Carrière, Bruce E Tabashnik, Jeffrey A Fabrick
BACKGROUND: The pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) is one of the world's most important pests of cotton. Insecticide sprays and transgenic cotton producing toxins of the bacterium Bacillus thuringiensis (Bt) are currently used to manage this pest. Bt toxins kill susceptible insects by specifically binding to and destroying midgut cells, but they are not toxic to most other organisms. Pink bollworm is useful as a model for understanding insect responses to Bt toxins, yet advances in understanding at the molecular level have been limited because basic genomic information is lacking for this cosmopolitan pest...
2016: GigaScience
https://www.readbyqxmd.com/read/27190043/molecular-cloning-expression-and-identification-of-bre-genes-involved-in-glycosphingolipids-synthesis-in-helicoverpa-armigera-lepidoptera-noctuidae
#4
Dandan Zhang, Yutao Xiao, Khalid Hussain Dhiloo, Mario Soberon, Alejandra Bravo, Kongming Wu
Glycosphingolipids (GSLs) play important roles in the cellular biology of vertebrate and invertebrate organisms, such as cell differentiation, tumor metastasis, and cell coordination. GSLs also serve as receptors for different bacterial toxins. For example, in the nematode Caenorhabditis elegans, GSLs function as receptors of the insecticidal Cry toxins produced by Bacillus thuringiensis (Bt), and mutations in bre genes involved in GSLs synthesis resulted in resistance to Cry5 toxin in this organism. However, the information of GSLs function in insects is still limited...
May 17, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/26935135/bacterial-vegetative-insecticidal-proteins-vip-from-entomopathogenic-bacteria
#5
REVIEW
Maissa Chakroun, Núria Banyuls, Yolanda Bel, Baltasar Escriche, Juan Ferré
Entomopathogenic bacteria produce insecticidal proteins that accumulate in inclusion bodies or parasporal crystals (such as the Cry and Cyt proteins) as well as insecticidal proteins that are secreted into the culture medium. Among the latter are the Vip proteins, which are divided into four families according to their amino acid identity. The Vip1 and Vip2 proteins act as binary toxins and are toxic to some members of the Coleoptera and Hemiptera. The Vip1 component is thought to bind to receptors in the membrane of the insect midgut, and the Vip2 component enters the cell, where it displays its ADP-ribosyltransferase activity against actin, preventing microfilament formation...
June 2016: Microbiology and Molecular Biology Reviews: MMBR
https://www.readbyqxmd.com/read/26163665/cloning-and-tissue-specific-expression-of-a-chitin-deacetylase-gene-from-helicoverpa-armigera-lepidoptera-noctuidae-and-its-response-to-bacillus-thuringiensis
#6
Guoying Han, Xiumin Li, Ting Zhang, Xiaoting Zhu, Jigang Li
Chitin deacetylases (CDAs) convert chitin into chitosan, the N-deacetylated form of chitin, which influences the mechanical and permeability properties of structures such as the cuticle and peritrophic matrices. In this article, a new CDA encoding gene, Hacda2, was cloned by reverse transcription-polymerase chain reaction method in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), with an open reading frame of 1,611 bp. The deduced protein composed of 536 amino acid residues with a signal peptide, a chitin-binding domain, a low-density lipoprotein receptor class A domain, and a polysaccharide deacetylase-like catalytic domain...
2015: Journal of Insect Science
https://www.readbyqxmd.com/read/24687616/construction-and-characterisation-of-near-isogenic-plutella-xylostella-lepidoptera-plutellidae-strains-resistant-to-cry1ac-toxin
#7
Xun Zhu, Yanyuan Lei, Yanjv Yang, Simon W Baxter, Jianhong Li, Qingjun Wu, Shaoli Wang, Wen Xie, Zhaojiang Guo, Wei Fu, Youjun Zhang
BACKGROUND: Resistance to insecticidal Bacillus thuringiensis (Bt) toxins has arisen in multiple populations of the worldwide Brassica pest Plutella xylostella (L.). To help elucidate the mechanism of resistance to Bt Cry1Ac toxin in a population from Florida, two pairs of near-isogenic lines (NILs) were developed. RESULTS: NILs were generated using either backcross or recombinant inbred line methodologies and evaluated for near-isogenicity with inter-simple-sequence-repeat (ISSR) markers...
February 2015: Pest Management Science
https://www.readbyqxmd.com/read/24498753/involvement-of-nonbinding-site-proteinases-in-the-development-of-resistance-of-helicoverpa-armigera-lepidoptera-noctuidae-to-cry1ac
#8
Guangchun Cao, Lili Zhang, Gemei Liang, Xianchun Li, Kongming Wu
Development of resistance to transgenic crops expressing the Cry toxin from Bacterium thuringiensis (Bt) has been the major concern for the long-term success of Bt crops. Alterations in nonbinding site proteinases and Bt toxin receptors are the two types of mechanisms responsible for Bt resistance in resistant insects. However, little is known about the relative contributions of the two types of mechanisms in the early and late phases of the development of Bt resistance. To address the relative contributions of four nonbinding site proteinases including esterase, total protease, chymotrypsin, and glutathione S-transferase in the early and late phases of the development of Cry1Ac resistance, we analyzed the relationships between nonbinding site proteinases and resistance of three groups of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) strains with different resistance levels because of different geographic origins and selection pressures...
December 2013: Journal of Economic Entomology
https://www.readbyqxmd.com/read/24355805/synthetic-fusion-protein-containing-domains-of-bt-cry1ac-and-allium-sativum-lectin-asal-conferred-enhanced-insecticidal-activity-against-major-lepidopteran-pests
#9
Sunita Tajne, Dayakar Boddupally, Vijayakumar Sadumpati, Dashavantha Reddy Vudem, Venkateswara Rao Khareedu
Different transgenic crop plants, developed with δ-endotoxins of Bacillus thuringiensis (Bt) and mannose-specific plant lectins, exhibited significant protection against chewing and sucking insects. In the present study, a synthetic gene (cry-asal) encoding the fusion-protein having 488 amino acids, comprising DI and DII domains from Bt Cry1Ac and Allium sativum agglutinin (ASAL), was cloned and expressed in Escherichia coli. Ligand blot analysis disclosed that the fusion-protein could bind to more number of receptors of brush border membrane vesicle (BBMV) proteins of Helicoverpa armigera...
February 10, 2014: Journal of Biotechnology
https://www.readbyqxmd.com/read/24286860/structural-evolutionary-and-functional-analysis-of-apn-genes-in-the-lepidoptera-bombyx-mori
#10
Ping Lin, Tingcai Cheng, Shengkai Jin, Liang Jiang, Chen Wang, Qingyou Xia
Aminopeptidases N (APNs), the receptors of Bacillus thuringiensis (Bt) toxin in the lepidopteran midgut, are involved in the Bt pathogen infection mechanism. In the present work, we screened 102 APNs from SilkDB, ButterflyBase and MonarchBase; 16 APNs were identified from the silkworm (Bombyx mori) and 24 from the monarch butterfly (Danaus plexippus). Syntenic and phylogenetic tree analysis showed that APN genes have developed multi-family genes before evolutionary divergence of the Lepidoptera. The tissue-expression pattern shows some BmAPNs are specifically or highly expressed in the midgut...
February 10, 2014: Gene
https://www.readbyqxmd.com/read/24195006/receptors-and-lethal-effect-of-bacillus-thuringiensis-insecticidal-crystal-proteins-to-the-anticarsia-gemmatalis-lepidoptera-noctuidae
#11
Lidia Mariana Fiuza, Neiva Knaak, Rogério Fernando Pires da Silva, João Antônio Pêgas Henriques
Bioassays with insecticidal crystal proteins (ICPs) from Bacillus thuringiensis have demonstrated that Cry1Aa, Cry1Ac, and Cry1Ba are the most active toxins on larvae of the Anticarsia gemmatalis. The toxins Cry1Da and Cry1Ea are less toxic, and toxins Cry2Aa are not active. Binding of these ICPs to midgut sections of the A. gemmatalis larvae was studied using streptavidin-mediated detection. The observed staining patterns showed that Cry1Aa and Cry1Ac bound to the brush border throughout the whole length of the midgut...
2013: ISRN Microbiology
https://www.readbyqxmd.com/read/24014526/efficient-production-of-bacillus-thuringiensis-cry1amod-toxins-under-regulation-of-cry3aa-promoter-and-single-cysteine-mutations-in-the-protoxin-region
#12
Blanca I García-Gómez, Jorge Sánchez, Diana L Martínez de Castro, Jorge E Ibarra, Alejandra Bravo, Mario Soberón
Bacillus thuringiensis Cry1AbMod toxins are engineered versions of Cry1Ab that lack the amino-terminal end, including domain I helix α-1 and part of helix α-2. This deletion improves oligomerization of these toxins in solution in the absence of cadherin receptor and counters resistance to Cry1A toxins in different lepidopteran insects, suggesting that oligomerization plays a major role in their toxicity. However, Cry1AbMod toxins are toxic to Escherichia coli cells, since the cry1A promoter that drives its expression in B...
November 2013: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/23999243/comparative-binding-of-cry1ab-and-cry1f-bacillus-thuringiensis-toxins-to-brush-border-membrane-proteins-from-ostrinia-nubilalis-ostrinia-furnacalis-and-diatraea-saccharalis-lepidoptera-crambidae-midgut-tissue
#13
COMPARATIVE STUDY
Sek Yee Tan, Bonifacio F Cayabyab, Edwin P Alcantara, Fangneng Huang, Kanglai He, Kenneth W Nickerson, Blair D Siegfried
The European (Ostrinia nubilalis Hübner) and Asian corn borers (Ostrinia furnacalis Guenée) are closely related and display similar sensitivity to Cry1 toxins. In this study, we compared the binding patterns of Cry1Ab and Cry1F toxins between both Ostrinia spp., as well as the expression of putative cadherin- and aminopeptidase-N (APN)-like protein receptors. Additionally, cDNA sequences of these putative toxin receptors from both Ostrinia species were compared. Ligand blots for both species indicated a similar binding pattern for Cry1Ab with the strongest immunoreactive band at 260 kDa in both species...
November 2013: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/23999013/a-protein-engineering-of-bacillus-thuringiensis-%C3%AE-endotoxin-by-conjugating-with-4-o-succinoyl-abamectin
#14
Zhi-Zhen Pan, Yu-Jing Zhu, Zheng Chen, Chuan-Qing Ruan, Lian Xu, Qing-Xi Chen, Bo Liu
Conjugation of Bacillus thuringiensis δ-endotoxin (Bt toxin) with other toxins for insect pest control has been proposed as a new efficient strategy with increasing insecticidal toxicity and target range and delay the onset of insect resistance. A modified method was investigated by conjugating Bt toxin with 4"-O-succinoyl abamectin to form a new biocide which was named as BtA. 'Zero-length' cross-linker EDC in combination with NHS activated 4"-O-succinoyl abamectin and extended half-life period of active intermediate for binding to Bt toxin...
November 2013: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/23933214/midgut-aminopeptidase-n-isoforms-from-ostrinia-nubilalis-activity-characterization-and-differential-binding-to-cry1ab-and-cry1fa-proteins-from-bacillus-thuringiensis
#15
Cristina M Crava, Yolanda Bel, Agata K Jakubowska, Juan Ferré, Baltasar Escriche
Aminopeptidase N (APN) isoforms from Lepidoptera are known for their involvement in the mode of action of insecticidal Cry proteins from Bacillus thuringiensis. These enzymes belong to a protein family with at least eight different members that are expressed simultaneously in the midgut of lepidopteran larvae. Here, we focus on the characterization of the APNs from Ostrinia nubilalis (OnAPNs) to identify potential Cry receptors. We expressed OnAPNs in insect cells using a baculovirus system and analyzed their enzymatic activity by probing substrate specificity and inhibitor susceptibility...
October 2013: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/23907718/molecular-characterization-of-a-short-peptidoglycan-recognition-protein-pgrp-s-from-asian-corn-borer-ostrinia-furnacalis-and-its-role-in-triggering-propo-activity
#16
Qiang Sun, Xiao-Xia Xu, Shoaib Freed, Wan-Jun Huang, Zhihua Zheng, Shuang Wang, Shun-Xiang Ren, Feng-Liang Jin
Peptidoglycan recognition proteins (PGRPs) are non-specific immune molecules of insects, and vertebrates etc., but are not present in plants and nematodes. In the current experiment, a PGRP DNA sequence (2,910 bp containing four exons) was identified from genomic DNA library of Asian corn borer, Ostrinia furnacalis, and a full-length cDNA programming PGRP was cloned (designed as OfPGRP-S) with an open reading frame of 579 bp, having 192 amino acid. This inferred amino acid sequence showed maximum similarity to known lepidopteran PGRPs...
January 2014: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/23748999/a-proteomic-approach-to-study-the-mechanism-of-tolerance-to-bt-toxins-in-ostrinia-furnacalis-larvae-selected-for-resistance-to-cry1ab
#17
Lina Xu, Natalie Ferry, Zhenying Wang, Jie Zhang, Martin G Edwards, Angharad M R Gatehouse, Kanglai He
A Cry1Ab-resistant population of Asian corn borer (ACB-AbR) exhibiting approximately 100 times greater resistance to activated Cry1Ab than a susceptible population (Ostrinia furnacalis; ACB-BtS), was previously shown to exhibit high levels of cross-resistance to Cry1Ah (131-fold), but no cross-resistance to Cry1Ie. It was suggested that the proposed mechanism of resistance was due to the alteration of specific receptors for Cry toxins in the midgut brush border membrane. In the present study a proteomic-based approach was used to identify proteins from brush border membrane vesicles (isolated from both resistant and susceptible Ostrinia furnacalis larvae) interacting with biotinylated Cry1Ab, Cry1Ah, and Cry1Ie...
December 2013: Transgenic Research
https://www.readbyqxmd.com/read/23747825/response-of-larval-ephestia-kuehniella-lepidoptera-pyralidae-to-individual-bacillus-thuringiensis-kurstaki-toxins-mixed-with-xenorhabdus-nematophila
#18
Dalel BenFarhat, Mariam Dammak, Saoussen Ben Khedher, Salima Mahfoudh, Schema Kammoun, Slim Tounsi
Bacillus thuringiensis kurstaki strain BNS3 produces parasporal crystals formed by Cry1Aa, Cry1Ac and Cry2Aa delta-endotoxins. In a previous work, we showed that the latter exhibited individually, a weak insecticidal activity against Ephestia kuehniella. In order to improve their toxicities, we studied the combined effect of each delta-endotoxin with X. nematophila cells on E. kuehniella larvae growth. Xenorhabdus cells were used in combination with spore crystal mixture of the wild strain BNS3, known to be active against E...
September 2013: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/23743444/sequence-variation-and-differential-splicing-of-the-midgut-cadherin-gene-in-trichoplusia-ni
#19
COMPARATIVE STUDY
Xin Zhang, Wendy Kain, Ping Wang
The insect midgut cadherin serves as an important receptor for the Cry toxins from Bacillus thuringiensis (Bt). Variation of the cadherin in insect populations provides a genetic potential for development of cadherin-based Bt resistance in insect populations. Sequence analysis of the cadherin from the cabbage looper, Trichoplusia ni, together with cadherins from 18 other lepidopterans showed a similar phylogenetic relationship of the cadherins to the phylogeny of Lepidoptera. The midgut cadherin in three laboratory populations of T...
August 2013: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/23645668/sodium-solute-symporter-and-cadherin-proteins-act-as-bacillus-thuringiensis-cry3ba-toxin-functional-receptors-in-tribolium-castaneum
#20
Estefanía Contreras, Michael Schoppmeier, M Dolores Real, Carolina Rausell
Understanding how Bacillus thuringiensis (Bt) toxins interact with proteins in the midgut of susceptible coleopteran insects is crucial to fully explain the molecular bases of Bt specificity and insecticidal action. In this work, aminopeptidase N (TcAPN-I), E-cadherin (TcCad1), and sodium solute symporter (TcSSS) have been identified by ligand blot as putative Cry3Ba toxin-binding proteins in Tribolium castaneum (Tc) larvae. RNA interference knockdown of TcCad1 or TcSSS proteins resulted in decreased susceptibility to Cry3Ba toxin, demonstrating the Cry toxin receptor functionality for these proteins...
June 21, 2013: Journal of Biological Chemistry
keyword
keyword
53812
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"