keyword
MENU ▼
Read by QxMD icon Read
search

Nannochloropsis

keyword
https://www.readbyqxmd.com/read/28634124/cell-disruption-and-lipid-extraction-for-microalgal-biorefineries-a-review
#1
REVIEW
Soo Youn Lee, Jun Muk Cho, Yong Keun Chang, You-Kwan Oh
The microalgae-based biorefinement process has attracted much attention from academic and industrial researchers attracted to its biofuel, food and nutraceutical applications. In this paper, recent developments in cell-disruption and lipid-extraction methods, focusing on four biotechnologically important microalgal species (namely, Chlamydomonas, Haematococcus, Chlorella, and Nannochloropsis spp.), are reviewed. The structural diversity and rigidity of microalgal cell walls complicate the development of efficient downstream processing methods for cell-disruption and subsequent recovery of intracellular lipid and pigment components...
June 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28628983/immobilized-oleaginous-microalgae-for-production-of-lipid-and-phytoremediation-of-secondary-effluent-from-palm-oil-mill-in-fluidized-bed-photobioreactor
#2
Benjamas Cheirsilp, Tipawan Thawechai, Poonsuk Prasertsan
Oleaginous microalga Nannochloropsis sp. was immobilized in alginate gel beads and cultivated under optimal conditions that their growth and lipid production were comparable to those of free cells. The immobilized cells were used in phytoremediation of secondary effluent from palm oil mill and easily recovered by simple sieving method. The immobilized cells contributed to removal of nitrogen and phosphorus >90% and CO2 mitigation >99%. They also gave the biomass and lipid production of 1.300±0.050g/L and 0...
June 14, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28628130/lipid-production-in-nannochloropsis-gaditana-is-doubled-by-decreasing-expression-of-a-single-transcriptional-regulator
#3
Imad Ajjawi, John Verruto, Moena Aqui, Leah B Soriaga, Jennifer Coppersmith, Kathleen Kwok, Luke Peach, Elizabeth Orchard, Ryan Kalb, Weidong Xu, Tom J Carlson, Kristie Francis, Katie Konigsfeld, Judit Bartalis, Andrew Schultz, William Lambert, Ariel S Schwartz, Robert Brown, Eric R Moellering
Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce lipids have previously met with little success. We identified 20 transcription factors as putative negative regulators of lipid production by using RNA-seq analysis of N. gaditana during nitrogen deprivation...
June 19, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28624245/a-biorefinery-for-nannochloropsis-induction-harvesting-and-extraction-of-epa-rich-oil-and-high-value-protein
#4
REVIEW
Elvis T Chua, Peer M Schenk
Microalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of high-value oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products...
May 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28605577/a-toolkit-for-nannochloropsis-oceanica-ccmp1779-enables-gene-stacking-and-genetic-engineering-of-the-eicosapentaenoic-acid-pathway-for-enhanced-long-chain-polyunsaturated-fatty-acid-production
#5
Eric Poliner, Jane A Pulman, Krzysztof Zienkiewicz, Kevin Childs, Christoph Benning, Eva M Farré
Nannochloropsis oceanica is an oleaginous microalga rich in ω3long-chain polyunsaturated fatty acids (LC-PUFAs) content, in the form of eicosapentaenoic acid (EPA). We identified the enzymes involved in LC-PUFA biosynthesis in N. oceanica CCMP1779 and generated multigene expression vectors aiming at increasing LC-PUFA content in vivo. We isolated the cDNAs encoding four fatty acid desaturases (FAD) and determined their function by heterologous expression in S. cerevisiae. To increase the expression of multiple fatty acid desaturases in N...
June 12, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28578809/formulation-and-utilisation-of-spent-anaerobic-digestate-fluids-for-the-growth-and-product-formation-of-single-cell-algal-cultures-in-heterotrophic-and-autotrophic-conditions
#6
Alla Silkina, Myrto-Panagiota Zacharof, Guillaume Hery, Thibaud Nouvel, Robert W Lovitt
Spent anaerobically digested effluents of agricultural origin were collected and treated using membrane filtration to achieve three-large particle free-nutrient streams of N:P ratios of 16.53, 3.78 and 14.22. Three algal species were grown on these streams, achieving good levels of bioremediation of digester fluids simultaneously with biomass and associated end product formation. Nannochloropsis oceanica and Scenedesmus quadricuada, where proven highly effective in remediating the streams achieving ammonia and phosphate reduction over 60% while for Schizochytrium limacinum SR21 these serve as an ideal production medium for lipids and biomass reaching 16...
May 24, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28542203/correction-genome-functional-gene-annotation-and-nuclear-transformation-of-the-heterokont-oleaginous-alga-nannochloropsis-oceanica-ccmp1779
#7
Astrid Vieler, Guangxi Wu, Chia-Hong Tsai, Blair Bullard, Adam J Cornish, Christopher Harvey, Ida-Barbara Reca, Chelsea Thornburg, Rujira Achawanantakun, Christopher J Buehl, Michael S Campbell, David Cavalier, Kevin L Childs, Teresa J Clark, Rahul Deshpande, Erika Erickson, Ann Armenia Ferguson, Witawas Handee, Que Kong, Xiaobo Li, Bensheng Liu, Steven Lundback, Cheng Peng, Rebecca L Roston, Sanjaya, Jeffrey P Simpson, Allan TerBush, Jaruswan Warakanont, Simone Zäuner, Eva M Farre, Eric L Hegg, Ning Jiang, Min-Hao Kuo, Yan Lu, Krishna K Niyogi, John Ohlrogge, Katherine W Osteryoung, Yair Shachar-Hill, Barbara B Sears, Yanni Sun, Hideki Takahashi, Mark Yandell, Shin-Han Shiu, Christoph Benning
[This corrects the article DOI: 10.1371/journal.pgen.1003064.].
May 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28541809/sequential-adaptation-of-nannochloropsis-gaditana-to-table-olive-processing-water
#8
Antonio Serrano, Carmen Contreras, Gonzalo Ruiz-Filippi, Rafael Borja, Fernando G Fermoso
The main objective of this study was to evaluate the suitability of Nannochloropsis gaditana to grow by sequential adaptation to TOPW (Table olive processing water) at increased substrate concentrations (10-80%). Sequential adaptation allows growing Nannochloropsis gaditana up to 80% TOPW, although the maximum microalgae biomass productions were achieved for percentages of 20-40%, i.e. 0.308 ± 0.005 g VSS (Volatile Suspended Solids)/L. In all growth experiments, proteins were the majority compound in the grown microalgae biomass (0...
May 25, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28521230/energy-consumption-and-water-soluble-protein-release-by-cell-wall-disruption-of-nannochloropsis-gaditana
#9
C Safi, L Cabas Rodriguez, W J Mulder, N Engelen-Smit, W Spekking, L A M van den Broek, G Olivieri, L Sijtsma
Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5kWh.kg(-1)biomass). Enzymatic treatment required low energy input (<0.34kWh.kg(-1)biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28499880/photoprotection-strategies-of-the-alga-nannochloropsis-gaditana
#10
Volha U Chukhutsina, Rikard Fristedt, Tomas Morosinotto, Roberta Croce
Nannochloropsis spp. are algae with high potential for biotechnological applications due to their capacity to accumulate lipids. However, little is known about their photosynthetic apparatus and acclimation/photoprotective strategies. In this work, we studied the mechanisms of non-photochemical quenching (NPQ), the fast response to high light stress, in Nannochloropsis gaditana by "locking" the cells in six different states during quenching activation and relaxation. Combining biochemical analysis with time-resolved fluorescence spectroscopy, we correlated each NPQ state with the presence of two well-known NPQ components: de-epoxidized xanthophylls and stress-related antenna proteins (LHCXs)...
May 9, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28489589/inhibitory-effect-of-ethanol-extract-of-nannochloropsis-oceanica-on-lipopolysaccharide-induced-neuroinflammation-oxidative-stress-amyloidogensis-and-memory-impairment
#11
Ji Yeon Choi, Chul Ju Hwang, Hee Pom Lee, Hee Sik Kim, Sang-Bae Han, Jin Tae Hong
Oxidative stress and neuroinflammation is implicated in the pathogenesis and development of Alzheimer's disease (AD). Here, we investigated the suppressive possibility of ethanol extract of Nannochloropsis oceanica (N. oceanica) on memory deficiency along with the fundamental mechanisms in lipopolysaccharide (LPS)-treated mice model. Among several extracts of 32 marine microalgae, ethanol extract of N. oceanica showed the most significant inhibitory effect on nitric oxide (NO) generation, NF-κB activity and β-secretase activity in cultured BV-2 cells, neuronal cells and Raw 264...
April 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28439814/imaging-the-accumulated-intracellular-microalgal-lipids-as-a-response-to-temperature-stress
#12
Khaled N M Elsayed, Tatiana A Kolesnikova, Anja Noke, Gerd Klöck
Over the last few decades, many scientists considered microalgae as promising actors for future biofuels because of the high lipid productivity inside their cells. Moreover, much attention has been paid to algal lipids as they can be used in biodiesel production. In this study, we optimized the different suitable conditions such as incubation time, incubation temperature, Dimethylesulfoxide and Nile red concentrations of the lipophilic fluorescence dye Nile red as an excellent and fast vital stain to detect and quantify intracellular lipids...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28433900/production-of-new-human-milk-fat-substitutes-by-enzymatic-acidolysis-of-microalgae-oils-from-nannochloropsis-oculata-and-isochrysis-galbana
#13
Yongjin He, Changyang Qiu, Zheng Guo, Jian Huang, Mingzi Wang, Bilian Chen
Human milk fat substitutes (HMFs) with four kinds of n-3 fatty acid for infant formula were firstly synthesized using triacylglycerols (TAGs) from Nannochloropsis oculata rich in PA at the sn-2 position and free fatty acids (FFAs) from Isochrysis galbana rich in n-3 polyunsaturated fatty acids (n-3 PUFAs-ALA/SDA/DHA) via solvent-free acidolysis with Novozym 435, Lipozyme 435, TL-IM and RM-IM as biocatalysts. The results show that the resulting HMFs contain total n-3 PUFA of 13.92-17.12% and PA of 59.38-68.13% at the sn-2 position under the optimal conditions (mole ratio FFAs/TAG 3:1, 60°C (Novozym 435 and Lipozyme TL-IM) and 50°C (Lipozyme 435 and RM-IM), lipase loading 10%, reaction time 24h)...
August 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28427282/biofouling-in-photobioreactors-for-marine-microalgae
#14
Ouassim Zeriouh, José Vicente Reinoso-Moreno, Lorenzo López-Rosales, María Del Carmen Cerón-García, Asterio Sánchez-Mirón, Francisco García-Camacho, Emilio Molina-Grima
The economic and/or energetic feasibility of processes based on using microalgae biomass requires an efficient cultivation system. In photobioreactors (PBRs), the adhesion of microalgae to the transparent PBR surfaces leads to biofouling and reduces the solar radiation penetrating the PBR. Light reduction within the PBR decreases biomass productivity and, therefore, the photosynthetic efficiency of the cultivation system. Additionally, PBR biofouling leads to a series of further undesirable events including changes in cell pigmentation, culture degradation, and contamination by invasive microorganisms; all of which can result in the cultivation process having to be stopped...
April 20, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28423307/aspects-of-phosphorus-physiology-associated-with-phosphate-induced-polar-lipid-remodelling-in-marine-microalgae
#15
José Pedro Cañavate, Isabel Armada, Ismael Hachero-Cruzado
Marine microalgae exhibit a diversified phosphorus physiology and have also been recently found to show high inter-taxa variability in their phosphate induced-polar lipids' remodelling. Identification of phosphorus physiology aspects that are more related to lipid remodelling can contribute to better understanding of such intricate phytoplankton lipid metabolism. Therefore, some aspects of phosphorus physiology related to its uptake, storage and use were evaluated in a taxonomically diversified group of nine marine microalgae that was arranged into three subgroups, each of them including species showing similar polar lipid responses to phosphate...
July 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28411052/selective-enrichment-of-eicosapentaenoic-acid-20-5n-3-in-n-oceanica-casa-cc201-by-natural-auxin-supplementation
#16
Aswathy Udayan, Muthu Arumugam
The present study aims to evaluate the effect of different concentration of natural auxin, Indole-3 acetic acid (IAA) on growth, lipid yield, PUFA and EPA accumulation in Nannochloropsis oceanica CASA CC201. It was observed that the, treatment with 10ppm concentration of IAA resulted in high cell number 579.5×10(6)cells/ml than the control (215.5×10(6)cells/ml). Treatment with IAA at a concentration of 40ppm gives the highest cellular lipid accumulation of 60.9% DCW than the control 31.05% DCW). Lipid yield is also found to be increased by the addition of 40ppm IAA (319...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28403199/the-plastoquinone-pool-of-nannochloropsis-oceanica-is-not-completely-reduced-during-bright-light-pulses
#17
Gunvor Røkke, Thor Bernt Melø, Martin Frank Hohmann-Marriott
The lipid-producing model alga Nannochloropsis oceanica has a distinct photosynthetic machinery. This organism possesses chlorophyll a as its only chlorophyll species, and has a high ratio of PSI to PSII. This high ratio of PSI to PSII may affect the redox state of the plastoquinone pool during exposure to light, and consequently may play a role in activating photoprotection mechanisms. We utilized pulse-amplitude modulated fluorometry to investigate the redox state of the plastoquinone pool during and after bright light pulses...
2017: PloS One
https://www.readbyqxmd.com/read/28396394/the-carbonic-anhydrase-cah1-is-an-essential-component-of-the-carbon-concentrating-mechanism-in-nannochloropsis-oceanica
#18
Christopher W Gee, Krishna K Niyogi
Aquatic photosynthetic organisms cope with low environmental CO2 concentrations through the action of carbon-concentrating mechanisms (CCMs). Known eukaryotic CCMs consist of inorganic carbon transporters and carbonic anhydrases (and other supporting components) that culminate in elevated [CO2] inside a chloroplastic Rubisco-containing structure called a pyrenoid. We set out to determine the molecular mechanisms underlying the CCM in the emerging model photosynthetic stramenopile, Nannochloropsis oceanica, a unicellular picoplanktonic alga that lacks a pyrenoid...
April 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28395932/microalgae-fractionation-using-steam-explosion-dynamic-and-tangential-cross-flow-membrane-filtration
#19
E Lorente, M Hapońska, E Clavero, C Torras, J Salvadó
In this study, the microalga Nannochloropsis gaditana was subjected to acid catalysed steam explosion treatment and the resulting exploded material was subsequently fractionated to separate the different fractions (lipids, sugars and solids). Conventional and vibrational membrane setups were used with several polymeric commercial membranes. Two different routes were followed: 1) filtration+lipid solvent extraction and 2) lipid solvent extraction+filtration. Route 1 revealed to be much better since the used membrane for filtration was able to permeate the sugar aqueous phase and retained the fraction containing lipids; after this, an extraction required a much lower amount of solvent and a better recovering yield...
March 24, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28352519/heterotrophic-cultivation-of-nannochloropsis-salina-for-enhancing-biomass-and-lipid-production
#20
Thangapandi Marudhupandi, Ramamoorthy Sathishkumar, Thipramalai Thankappan Ajith Kumar
Response surface methodology (RSM) was used to enhance the biomass and lipid content in Nannochloropsis salina due to its economic importance. Preliminary screening results revealed that the heterotrophically cultivated N. salina with various carbon and nitrogen sources yielded higher biomass (0.91 ± 0.0035 g/L) and lipid content (37.1 ± 0.49 mg/L) than that of the photoautotrophical cultivation (0.21 ± 0.009 g/L and 22.16 ± 0.27 mg/L). Significant sources that greatly influenced on biomass and lipid content of the alga were optimized through RSM...
June 2016: Biotechnology Reports
keyword
keyword
53779
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"