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Nannochloropsis

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https://www.readbyqxmd.com/read/28070221/nannochloropsis-a-rich-source-of-diacylglycerol-acyltransferases-for-engineering-of-triacylglycerol-content-in-different-hosts
#1
Krzysztof Zienkiewicz, Agnieszka Zienkiewicz, Eric Poliner, Zhi-Yan Du, Katharina Vollheyde, Cornelia Herrfurth, Sofia Marmon, Eva M Farré, Ivo Feussner, Christoph Benning
BACKGROUND: Photosynthetic microalgae are considered a viable and sustainable resource for biofuel feedstocks, because they can produce higher biomass per land area than plants and can be grown on non-arable land. Among many microalgae considered for biofuel production, Nannochloropsis oceanica (CCMP1779) is particularly promising, because following nutrient deprivation it produces very high amounts of triacylglycerols (TAG). The committed step in TAG synthesis is catalyzed by acyl-CoA:diacylglycerol acyltransferase (DGAT)...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28061396/effective-harvesting-of-microalgae-comparison-of-different-polymeric-flocculants
#2
Yoram Gerchman, Barak Vasker, Mordechai Tavasi, Yael Mishael, Yael Kinel-Tahan, Yaron Yehoshua
Microalgae harvesting is a major hurdle in the use of microalgae for oil production. Here we describe the use of a standard cationic polymer used for water treatment, Polydiallyldimethylammonium chloride (PDADMAC), for sedimentation of Chlorella vulgaris and comparison of its flocculation properties with two other polymers, chitosan and Superfloc®. We found PDADMAC to be the most effective flocculant with 90% of the algae flocculating at concentrations as low as 5mg/L within 60min, and good activity even at pH=10...
December 21, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28057409/detoxification-of-ammonium-to-nannochloropsis-oculata-and-enhancement-of-lipid-production-by-mixotrophic-growth-with-acetate
#3
Weitie Lin, Pengfei Li, Zipeng Liao, Jianfei Luo
In this study, the toxicity of ammonium was removed in the microalga Nannochloropsis oculata by using acetate as a carbon source. Algal biomass and lipid production were significantly enhanced when N. oculata was grew on 0.5-50mM of ammonium and 16-64mM of acetate in mixotrophic conditions. When grown mixotrophically on 1mM of ammonium and 32mM of acetate, the biomass and lipid production reached 543mg/L and 279mg/L respectively, which were 1.5 and 9.4times higher than the levels generated when grown autotrophically on nitrate...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27988475/two-stage-cultivation-of-nannochloropsis-oculata-for-lipid-production-using-reversible-alkaline-flocculation
#4
Gibran Sidney Aléman-Nava, Koenraad Muylaert, Sara Paulina Cuellar Bermudez, Orily Depraetere, Bruce Rittmann, Roberto Parra-Saldívar, Dries Vandamme
Two-stage cultivation for microalgae biomass is a promising strategy to boost lipid accumulation and productivity. Most of the currently described processes use energy-intensive centrifugation for cell separation after the first cultivation stage. This laboratory study evaluated alkaline flocculation as low-cost alternative separation method to harvest Nannochloropsis oculata prior to cultivation in the second nutrient-depleted cultivation stage. Biomass concentration over time and the maximum quantum yield of photosystem II expressed as Fv:Fm ratio showed identical patterns for both harvesting methods in both stages...
February 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27957653/harvesting-of-the-microalga-nannochloropsis-sp-by-bioflocculation-with-mung-bean-protein-extract
#5
Ganesan Kandasamy, Sitti Raehanah Muhamad Shaleh
Harvesting microalgae from medium is a major challenge due to their small size and low concentrations. In an attempt to find a cost-effective and eco-friendly harvesting technique, mung bean (Vigna radiata) protein extract (MBPE) was used for flocculation of Nannochloropsis sp. The effects of parameters such as pH, flocculant dose, algae concentration, and mixing time were used to study the flocculation efficiency (FE) of MBPE. Optimum parameters of MBPE dosage of 20 mL L(-1) and a mixing rate of 300 rpm for 6 min achieved a FE of >92% after 2 h of settling time...
December 12, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27939996/effects-of-environmentally-relevant-antibiotic-mixtures-on-marine-microalgal-growth
#6
Jaclyn R Teixeira, Elise F Granek
As of 2008, approximately 48% of Americans use prescription drugs within any given 30-day period. Many pharmaceutical compounds are not fully metabolized by the human body, nor fully removed by wastewater treatment systems, before release into the environment. As a result, a vast array of pharmaceuticals has been detected in marine and freshwater organisms, sediments, and waters, with unintended effects on non-target organisms, and limited studies of environmental effects. The antibiotics sulfamethoxazole (SMX), and trimethoprim (TRI), often prescribed together to treat bacterial infections, have been detected worldwide in marine and estuarine environments at concentrations up to 765-870ng/L each...
December 8, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27895203/a-palmitic-acid-elongase-affects-eicosapentaenoic-acid-and-plastidial-monogalactosyldiacylglycerol-levels-in-nannochloropsis
#7
Lina-Juana Dolch, Camille Rak, Giorgio Perin, Guillaume Tourcier, Richard Broughton, Marina Leterrier, Tomas Morosinotto, Frédérique Tellier, Jean-Denis Faure, Denis Falconet, Juliette Jouhet, Olga Sayanova, Frédéric Beaudoin, Eric Maréchal
Nannochloropsis species are oleaginous eukaryotes containing a plastid limited by four membranes, deriving from a secondary endosymbiosis. In Nannochloropsis, thylakoid lipids, including monogalactosyldiacylglycerol (MGDG), are enriched in eicosapentaenoic acid (EPA). The need for EPA in MGDG is not understood. Fatty acids are de novo synthesized in the stroma, then converted into very-long-chain polyunsaturated fatty acids (FAs) at the endoplasmic reticulum (ER). The production of MGDG relies therefore on an EPA supply from the ER to the plastid, following an unknown process...
2017: Plant Physiology
https://www.readbyqxmd.com/read/27888732/biorefinery-of-microalgal-soluble-proteins-by-sequential-processing-and-membrane-filtration
#8
C Safi, G Olivieri, R P Campos, N Engelen-Smit, W J Mulder, L A M van den Broek, L Sijtsma
A mild biorefinery process was investigated on the microalga Nannochloropsis gaditana, to obtain an enriched fraction of water soluble proteins free from chlorophyll. After harvesting, a 100g.L(-1) solution of cells was first subjected to cell disruption by either high-pressure homogenization (HPH) or enzymatic treatment (ENZ). HPH resulted in a larger release of proteins (49%) in the aqueous phase compared to the Alcalase incubation (35%). In both cases, an ultrafiltration/diafiltration (UF/DF) was then performed on the supernatant obtained from cell disruption by testing different membrane cut-off (1000kDa, 500kDa and 300kDa)...
February 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27849022/nitrogen-induced-metabolic-changes-and-molecular-determinants-of-carbon-allocation-in-dunaliella-tertiolecta
#9
Kenneth Wei Min Tan, Huixin Lin, Hui Shen, Yuan Kun Lee
Certain species of microalgae are natural accumulators of lipids, while others are more inclined to store starch. However, what governs the preference to store lipids or starch is not well understood. In this study, the microalga Dunaliella tertiolecta was used as a model to study the global gene expression profile regulating starch accumulation in microalgae. D. tertiolecta, when depleted of nitrogen, produced only 1% of dry cell weight (DCW) in neutral lipids, while starch was rapidly accumulated up to 46% DCW...
November 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27806886/aqueous-enzymatic-process-for-cell-wall-degradation-and-lipid-extraction-from-nannochloropsis-sp
#10
Chongchong Wu, Ye Xiao, Weiguo Lin, Jiaquan Li, Saisai Zhang, Junying Zhu, Junfeng Rong
An effective cell disruption method, including alkaline pretreatment and subsequent enzymatic treatment, was established to break cell walls and extract lipid from Nannochloropsis sp. A synergistic effect was found between alkaline pretreatment and enzymatic treatment. The combination of commercialize enzymes (cellulase, protease, lysozyme, and pectinase) achieved higher lipid yield compared with a single enzyme application. With the compromise between economic feasibility and lipid yield, the optimum reaction conditions were obtained with alkaline pretreatment at pH 10...
January 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27778140/nitrogen-deprivation-of-microalgae-effect-on-cell-size-cell-wall-thickness-cell-strength-and-resistance-to-mechanical-disruption
#11
Benjamin H J Yap, Simon A Crawford, Raymond R Dagastine, Peter J Scales, Gregory J O Martin
Nitrogen deprivation (N-deprivation) is a proven strategy for inducing triacylglyceride accumulation in microalgae. However, its effect on the physical properties of cells and subsequently on product recovery processes is relatively unknown. In this study, the effect of N-deprivation on the cell size, cell wall thickness, and mechanical strength of three microalgae was investigated. As determined by analysis of micrographs from transmission electron microscopy, the average cell size and cell wall thickness for N-deprived Nannochloropsis sp...
October 24, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27734239/modular-antenna-of-photosystem-i-in-secondary-plastids-of-red-algal-origin-a-nannochloropsis-oceanica-case-study
#12
David Bína, Zdenko Gardian, Miroslava Herbstová, Radek Litvín
Photosystem I (PSI) is a multi-subunit integral pigment-protein complex that performs light-driven electron transfer from plastocyanin to ferredoxin in the thylakoid membrane of oxygenic photoautotrophs. In order to achieve the optimal photosynthetic performance under ambient irradiance, the absorption cross section of PSI is extended by means of peripheral antenna complexes. In eukaryotes, this role is played mostly by the pigment-protein complexes of the LHC family. The structure of the PSI-antenna supercomplexes has been relatively well understood in organisms harboring the primary plastid: red algae, green algae and plants...
October 12, 2016: Photosynthesis Research
https://www.readbyqxmd.com/read/27725210/production-of-eicosapentaenoic-acid-by-nannochloropsis-oculata-effects-of-carbon-dioxide-and-glycerol
#13
Carolina Shene, Yusuf Chisti, Daniela Vergara, César Burgos-Díaz, Mónica Rubilar, Mariela Bustamante
The marine microalga Nannochloropsis oculata is a potential source of eicosapentaenoic acid (EPA, C20:5n3) and carotenoids for use in functional foods and nutraceuticals. Mixotrophic culture of N. oculata using glycerol was examined as a possible way of increasing the biomass and metabolite productivity relative to a pure photoautotrophic culture in modified f/2 medium. The effect of CO2 supply was also tested. EPA production in semi-continuous culture with and without glycerol and CO2 was evaluated. The effects of glycerol supplementation and light/dark cycling on the production of the biomass and EPA are reported for cultures conducted at a constant pH controlled using CO2...
December 10, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27712934/effects-of-tio2-nanoparticles-and-sunscreens-on-coastal-marine-microalgae-ultraviolet-radiation-is-key-variable-for-toxicity-assessment
#14
M Sendra, D Sánchez-Quiles, J Blasco, I Moreno-Garrido, L M Lubián, S Pérez-García, A Tovar-Sánchez
Given the large numbers of sunbathers on beaches, sunscreen compounds are being released into the coastal aquatic environment in significant amounts. Until now the effect of these potential pollutants on microbiota has been not well-known. Phytoplankton is a key component of the microbiota community. It forms the basis of the aquatic trophic networks, and any change in the natural population of phytoplankton can affect the structure of aquatic biota. This paper describes an experiment performed outdoors (in natural sunlight conditions including ultraviolet radiation (UVR) and with UVR blocked) on mixed microalgae populations (four species from different key marine taxonomic groups, Nannochloropsis gaditana, Chaetoceros gracilis, Pleurochrysis roscoffensis and Amphidinium carterae), for three days, exposed to a range of concentrations of three commercial sunscreens (with variable TiO2 concentrations: highest concentration for sunscreen C, followed by sunscreen A; and sunscreen B did not contain TiO2 in its composition)...
October 4, 2016: Environment International
https://www.readbyqxmd.com/read/27665531/augmentation-of-arsenic-enhances-lipid-yield-and-defense-responses-in-alga-nannochloropsis-sp
#15
A K Upadhyay, S K Mandotra, N Kumar, N K Singh, Lav Singh, U N Rai
The present study was conducted with microalga Nannochloropsis sp. to evaluate its tolerance responses and biofuel perspectives under different arsenic regime (0-1000μM As(III)). Results showed that optimal biomass (61.00±3.72mg/L/d) and lipid productivity (20.27mg/L/d) were obtained in culture treated with 100μM As(III) in comparison to other treatments. In addition, fatty acid profile of alga was in accordance with European biodiesel standards (EN 14214), which reflects good oxidative stability of oil...
December 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27660987/life-cycle-assessment-of-microalgae-based-aviation-fuel-influence-of-lipid-content-with-specific-productivity-and-nitrogen-nutrient-effects
#16
Fang Guo, Jing Zhao, Lusi A, Xiaoyi Yang
The aim of this work is to compare the life cycle assessments of low-N and normal culture conditions for a balance between the lipid content and specific productivity. In order to achieve the potential contribution of lipid content to the life cycle assessment, this study established relationships between lipid content (nitrogen effect) and specific productivity based on three microalgae strains including Chlorella, Isochrysis and Nannochloropsis. For microalgae-based aviation fuel, the effects of the lipid content on fossil fuel consumption and greenhouse gas (GHG) emissions are similar...
December 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27649964/lipids-rich-in-%C3%AF-3-polyunsaturated-fatty-acids-from-microalgae
#17
REVIEW
N F Santos-Sánchez, R Valadez-Blanco, B Hernández-Carlos, A Torres-Ariño, P C Guadarrama-Mendoza, R Salas-Coronado
Despite microalgae recently receiving enormous attention as a potential source of biodiesel, their use is still not feasible as an alternative to fossil fuels. Recently, interest in microalgae has focused on the production of bioactive compounds such as polyunsaturated fatty acids (PUFA), which provide microalgae a high added value. Several considerations need to be assessed for optimizing PUFA production from microalgae. Firstly, a microalgae species that produces high PUFA concentrations should be selected, such as Nannochloropsis gaditana, Isochrysis galbana, Phaeodactylum tricornutum, and Crypthecodinium cohnii, with marine species gaining more attention than do freshwater species...
October 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27620972/conservation-of-core-complex-subunits-shaped-the-structure-and-function-of-photosystem-i-in-the-secondary-endosymbiont-alga-nannochloropsis-gaditana
#18
Alessandro Alboresi, Clotilde Le Quiniou, Sathish K N Yadav, Martin Scholz, Andrea Meneghesso, Caterina Gerotto, Diana Simionato, Michael Hippler, Egbert J Boekema, Roberta Croce, Tomas Morosinotto
Photosystem I (PSI) is a pigment protein complex catalyzing the light-driven electron transport from plastocyanin to ferredoxin in oxygenic photosynthetic organisms. Several PSI subunits are highly conserved in cyanobacteria, algae and plants, whereas others are distributed differentially in the various organisms. Here we characterized the structural and functional properties of PSI purified from the heterokont alga Nannochloropsis gaditana, showing that it is organized as a supercomplex including a core complex and an outer antenna, as in plants and other eukaryotic algae...
January 2017: New Phytologist
https://www.readbyqxmd.com/read/27611030/flocculation-properties-of-several-microalgae-and-a-cyanobacterium-species-during-ferric-chloride-chitosan-and-alkaline-flocculation
#19
Sanjaya Lama, Koenraad Muylaert, Tika Bahadur Karki, Imogen Foubert, Rita K Henderson, Dries Vandamme
Flocculation holds great potential as a low-cost harvesting method for microalgae biomass production. Three flocculation methods (ferric chloride, chitosan, and alkaline flocculation) were compared in this study for the harvesting of 9 different freshwater and marine microalgae and one cyanobacterium species. Ferric chloride resulted in a separation efficiency greater than 90% with a concentration factor (CF) higher than 10 for all species. Chitosan flocculation worked generally very well for freshwater microalgae, but not for marine species...
November 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27572053/identification-of-a-malonyl-coa-acyl-carrier-protein-transacylase-and-its-regulatory-role-in-fatty-acid-biosynthesis-in-oleaginous-microalga-nannochloropsis-oceanica
#20
Jia-Wen Chen, Wan-Jun Liu, Dong-Xiong Hu, Xiang Wang, Srinivasan Balamurugan, Adili Alimujiang, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li
Oleaginous microalgae hold great promises for biofuel production. However, commercialization of microalgal biofuels remains impracticable due to lack of suitable industrial strain with high growth rate and lipid productivity. Engineering of metabolic pathways is a potential strategy for the improvement of microalgal strains for the production of lipids and also value-added products in microalgae. Malonyl CoA-acyl carrier protein transacylase (MCAT) has been reported to be involved in fatty acid biosynthesis...
August 30, 2016: Biotechnology and Applied Biochemistry
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