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Chun-Yen Chen, Dillirani Nagarajan, Wai Yan Cheah
In this study, Nannochloropsis oceanica CY2 was grown in deep-sea water (DSW)-based medium in 5-L plastic bag-type photobioreactors (PBRs) for the autotrophic production of Eicosapentaenoic acid (EPA, 20:5n-3). EPA production of N. oceanica CY2 was stimulated when it was grown in 100% DSW amended with 1.5 g L-1 NaNO3, achieving a EPA content of 3.1% and a biomass concentration of 3.3 g L-1. An outdoor-simulated microalgae cultivation system was also conducted to validate the feasibility of outdoor cultivation of the CY2 strain in plastic bag-type PBRs...
December 30, 2017: Bioresource Technology
Ruijuan Ma, Skye R Thomas-Hall, Elvis T Chua, Eladl Eltanahy, Michael E Netzel, Gabriele Netzel, Yinghua Lu, Peer M Schenk
The microalga Nannochloropsis produces high-value omega-3-rich fatty acids and carotenoids. In this study the effects of light intensity and wavelength on biomass, fatty acid, and carotenoid production with respect to light output efficiency were investigated. Similar biomass and fatty acid yields were obtained at high light intensity (150 μmol m-2 s-1) LEDs on day 7 and low light intensity (50 μmol m-2 s-1) LEDs on day 11 during cultivation, but the power efficiencies of biomass and fatty acid (specifically eicosapentaenoic acid) production were higher for low light intensity...
December 29, 2017: Bioresource Technology
Rajdeep Shakya, Sushil Adhikari, Ravishankar Mahadevan, El Barbary Hassan, Thomas A Dempster
Upgrading of bio-oil obtained from hydrothermal liquefaction (HTL) of algae is necessary for it to be used as a fuel. In this study, bio-oil obtained from HTL of Nannochloropsis sp. was upgraded using five different catalysts (Ni/C, ZSM-5, Ni/ZSM-5, Ru/C and Pt/C) at 300 °C and 350 °C. The upgraded bio-oil yields were higher at 300 °C; however, higher quality upgraded bio-oils were obtained at 350 °C. Ni/C gave the maximum upgraded bio-oil yield (61 wt%) at 350 °C. However, noble metal catalysts (Ru/C and Pt/C) gave the better upgraded bio-oils in terms of acidity, heating values, and nitrogen values...
December 21, 2017: Bioresource Technology
Chae Hun Ra, Phunlap Sirisuk, Jang-Hyun Jung, Gwi-Taek Jeong, Sung-Koo Kim
Integrations of two-phase culture for cell growth and lipid accumulation using mixed LED and green LED wavelengths were evaluated with the microalgae, Phaeodactylum tricornutum, Isochrysis galbana, Nannochloropsis salina, and Nannochloropsis oceanica. Among the single and mixed LED wavelengths, mixed LED produced higher biomass of the four microalgae, reaching 1.03 g DCW/L I. galbana, followed by 0.95 g DCW/L P. tricornutum, 0.85 g DCW/L N. salina, and 0.62 g DCW/L N. oceanica than single LED or fluorescent lights at day 10...
December 19, 2017: Bioprocess and Biosystems Engineering
Wenjia Wang, Youtong Xu, Xiaoxiao Wang, Bokun Zhang, Wenying Tian, Jinglai Zhang
Hydrothermal liquefaction (HTL) of microalgae Nannochloropsis (NAS) over various transition metal M/TiO2 (M = Fe, Co, Ni, Mo, and Mn) was investigated. Ni/TiO2 was the most effective catalyst to improve the yield and quality of biocrude and the liquefaction conversion. Ni/TiO2 was characterized by XRD, XRF, and XPS. The research of Effect of reaction temperature on HTL of NAS over Ni/TiO2 suggested that 300 °C led to a maximum biocrude yield of 48.23% and the highest liquefaction conversion of 89.28%...
November 20, 2017: Bioresource Technology
Yahui Sun, Yun Huang, Qiang Liao, Ao Xia, Qian Fu, Xun Zhu, Jingwei Fu
Aiming at alleviating the adverse effect of poor light penetrability on microalgae growth, planar waveguide modules functioned as diluting and redistributing the intense incident light within microalgae culture more homogeneously were introduced into a lab-scale open raceway pond (ORP) for Nannochloropsis oculata cultivation. As compared to the conventional ORP, the illumination surface area to volume ratio and effective illuminated volume percentage in the proposed ORP were respectively improved by 5.53 times and 19...
November 6, 2017: Bioresource Technology
Javier Ávila-Román, Elena Talero, Carolina de Los Reyes, Sofía García-Mauriño, Virginia Motilva
Oxylipins (OXLs) are bioactive molecules generated by the oxidation of fatty acids that promote the resolution of acute inflammation and prevent chronic inflammatory processes through molecular mechanisms that are not well known. We have previously reported the anti-inflammatory activity of microalgae-derived OXLs and OXL-containing biomass in two inflammatory bowel disease (IBD) models: 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis and TNBS-induced recurrent colitis. In this study, we examined the in vitro anti-inflammatory mechanism of action of the most abundant OXLs isolated from Chlamydomonas debaryana (13S-HOTE and 13S-HODE) and Nannochloropsis gaditana (15S-HEPE)...
November 9, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Giorgio Perin, Andrea Bernardi, Alessandra Bellan, Fabrizio Bezzo, Tomas Morosinotto
The optimization of algae biomass productivity in industrial cultivation systems requires genetic improvement of wild type strains isolated from nature. One of the main factors affecting algae productivity is their efficiency in converting light into chemical energy and this has been a major target of recent genetic efforts. However, photosynthetic productivity in algae cultures depends on many environmental parameters, making the identification of advantageous genotypes complex and the achievement of concrete improvements slow...
November 8, 2017: Metabolic Engineering
Karina Bravo-Tello, Nicole Ehrenfeld, Camila J Solís, Pilar E Ulloa, Manuel Hedrera, Marjorie Pizarro-Guajardo, Daniel Paredes-Sabja, Carmen G Feijóo
Soybean meal has been used in many commercial diets for farm fish; despite this component inducing intestinal inflammation. On the other hand, microalgae have increasingly been used as dietary supplements in fish feed. Nevertheless, the vast quantity of microalgae species means that many remain under- or unstudied, thus limiting wide scale commercial application. In this work, we evaluated the effects to zebrafish (Danio rerio) of including Tetraselmis sp (Ts); Phaeodactylum tricornutum (Pt); Chlorella sp (Ch); Nannochloropsis oculata (No); or Nannochloropsis gaditana (Ng) as additives in a soybean meal-based diet on intestinal inflammation and survival after Edwardsiella tarda infection...
2017: PloS One
Yi Xin, Yandu Lu, Yi-Ying Lee, Li Wei, Jing Jia, Qintao Wang, Dongmei Wang, Fali Bai, Hanhua Hu, Qiang Hu, Jin Liu, Yantao Li, Jian Xu
Microalgal oils, depending on their degree of unsaturation, can be utilized as either nutritional supplements or fuels; thus, a feedstock with genetically designed and tunable degree of unsaturation is desirable to maximize process efficiency and product versatility. Systematic profiling of ex vivo (in yeast), in vitro, and in vivo activities of type-2 diacylglycerol acyltransferases in Nannochloropsis oceanica (NoDGAT2s or NoDGTTs), via reverse genetics, revealed that NoDGAT2A prefers saturated fatty acids (SFAs), NoDGAT2D prefers monounsaturated fatty acids (MUFAs), and NoDGAT2C exhibits the strongest activity toward polyunsaturated fatty acids (PUFAs)...
December 4, 2017: Molecular Plant
Hyun-Ro Lee, KyuHan Kim, Sung Cik Mun, Yong Keun Chang, Siyoung Q Choi
Despite the enormous potential of cellulose nanofibrils (CNFs) as a reinforcing filler in various fields, the use of them has been limited by high-energy mechanical treatments that require a lot of energy and time consumption. To reduce the demands of energy and time required for mechanical treatments, microalgae, in particular, Nannochloropsis oceanica, which has small size, rapid growth rate, and high productivity was used as a CNFs source. This study obtains the CNFs by lipid/protein extraction, purification, and TEMPO-mediated oxidation processes under gentle mixing without high-energy mechanical treatments...
January 15, 2018: Carbohydrate Polymers
Gianluca Maffei, Maria Paola Bracciale, Alessandra Broggi, Antonio Zuorro, Maria Laura Santarelli, Roberto Lavecchia
The effects of an enzymatic treatment with cellulase and mannanase on the properties of marine microalgae Nannochloropsis sp. were investigated. The combined use of these enzymes synergistically promoted the recovery of lipids from the microalgae, increasing the extraction yield from 40.8 to over 73%. Untreated and enzymatically treated microalgae were characterized by chemical analysis and by TGA/DTG, FTIR, XRD and SEM. Significant changes were observed in the chemical composition and thermal behavior of the microalgae...
October 18, 2017: Bioresource Technology
Zhiqiang Wu, Wangcai Yang, Bolun Yang
In this work, the influence of Nannochloropsis and Chlorella on the thermal behavior and surface morphology of char during the co-pyrolysis process were explored. Thermogravimetric and iso-conversional methods were applied to analyzing the pyrolytic and kinetic characteristics for different mass ratios of microalgae and low-rank coal (0, 3:1, 1:1, 1:3 and 1). Fractal theory was used to quantitatively determine the effect of microalgae on the morphological texture of co-pyrolysis char. The result indicated that both the Nannochloropsis and Chlorella promoted the release of volatile from low-rank coal...
October 9, 2017: Bioresource Technology
Roopa Devasya, Amarjeet Bassi
Phyco-remediation is an environmental friendly method, which involves the application of beneficial microalgae to treat wastewater-containing pollutants for a diverse range of conditions. Several industrial processes generate hyper saline wastewater, which is a significant challenge for conventional wastewater treatment, and the disposal of saline waters also has a significant negative impact on the environment. Road salt run-off is one such saline wastewater stream not currently treated and one that contributes significantly to negatively impacting receiving bodies of water...
October 26, 2017: Environmental Technology
Alice Mühlroth, Per Winge, Aimen El Assimi, Juliette Jouhet, Eric Marechal, Martin F Hohmann-Marriott, Olav Vadstein, Atle M Bones
Molecular mechanisms of phosphorus (P) limitation are of great interest for understanding algal production in aquatic ecosystems. Previous studies point to P limitation induced changes in lipid composition. As in microalgae the molecular mechanisms of this specific P-stress adaptation remain unresolved, we reveal a detailed phospholipid-recycling scheme in Nannochloropsis oceanica and described important P acquisition genes based on highly corresponding transcriptome and lipidome data. Initial responses to P limitation showed increased expression of genes involved in P uptake and an expansion of P substrate spectrum based on purple acid phosphatases...
October 19, 2017: Plant Physiology
Nam Kyu Kang, Eun Kyung Kim, Young Uk Kim, Bongsoo Lee, Won-Joong Jeong, Byeong-Ryool Jeong, Yong Keun Chang
BACKGROUND: Genetic engineering of microalgae is necessary to produce economically feasible strains for biofuel production. Current efforts are focused on the manipulation of individual metabolic genes, but the outcomes are not sufficiently stable and/or efficient for large-scale production of biofuels and other materials. Transcription factors (TFs) are emerging as good alternatives for engineering of microalgae, not only to increase production of biomaterials but to enhance stress tolerance...
2017: Biotechnology for Biofuels
Paula A Lopes, Narcisa M Bandarra, Susana V Martins, Joana Martinho, Cristina M Alfaia, Marta S Madeira, Carlos Cardoso, Cláudia Afonso, Maria C Paulo, Rui M A Pinto, José L Guil-Guerrero, José A M Prates
BACKGROUND: To overcome the current overexploitation of fish rich in n-3 long chain polyunsaturated fatty acids (LCPUFA), microalgae have become a promising marine lipid source. The purpose of this study was to assess eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), isolated or combined from distinct marine origins, on the promotion of neuroprotective effects. METHODS: The experiment lasted for 10 weeks and involved 32 Wistar rats, divided into 4 diets (n = 8): a diet rich in milk fat was taken as control (Milk Fat) and compared to n-3 LCPUFA enriched diets, either in EPA + DHA form through fish oil (Fish Oil), or EPA through Nannochloropsis oil (Nanno), or DHA through Schizochytrium oil (Schyzo), while maintaining Milk Fat incorporation...
2017: Nutrition & Metabolism
Mohammed Fahad Hasan, Frank Vogt
Marine phytoplankton acts as a considerable sink of atmospheric CO2 as it sequesters large quantities of this greenhouse gas for biomass production. To assess microalgae's counterbalancing of global warming, the quantities of CO2 they fix need to be determined. For this task, it is mandatory to understand which environmental and physiological parameters govern this transformation from atmospheric CO2 to microalgal biomass. However, experimental analyses are challenging as it has been found that the chemical environment has a major impact on the physiological properties of the microalgae cells (diameter typ...
October 23, 2017: Analyst
Ritu Verma, Rahul Kumar, Luv Mehan, Aradhana Srivastava
Microalgae, a renewable source for third generation biofuel production, have a great potential if cultivated in high concentration economically. Bottleneck lies with designing economical and efficient photobioreactor. In addition, proportional C and N inputs in the known media does not support high specific growth rate and high biomass build-up. Nitrates in fermentation media, f/2 for Nannochloropsis sp. and Zarrouk's for Arthrospiraplatensis, were modified. Aeration and agitation were altered in conventional bioreactor (BIOFLO 110) to reduce power consumption, increase mixing time and prevents settling...
October 5, 2017: Journal of Bioscience and Bioengineering
Sohee Kwon, Nam Kyu Kang, Hyun Gi Koh, Sung-Eun Shin, Bongsoo Lee, Byeong-Ryool Jeong, Yong Keun Chang
Microalgae are considered as excellent platforms for biomaterial production that can replace conventional fossil fuel-based fuels and chemicals. Genetic engineering of microalgae is prerequisite to maximize production of materials and to reduce costs for the production. Transcription factors (TFs) are emerging as key regulators of metabolic pathways to enhance production of molecules for biofuels and other materials. TFs with the basic leucine zipper (bZIP) domain have been known as stress regulators and are associated with lipid metabolism in plants...
October 4, 2017: Biotechnology and Bioengineering
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