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https://www.readbyqxmd.com/read/29777821/regional-cardiac-function-analysis-from-tagged-mri-images-comparison-of-techniques-harmonic-phase-harp-versus-sinusoidal-modeling-sinmod-analysis
#1
El-Sayed H Ibrahim, Jadranka Stojanovska, Azza Hassanein, Claire Duvernoy, Pierre Croisille, Rodica Pop-Busui, Scott D Swanson
Cardiac MRI tagging is a valuable technique for evaluating regional heart function. Currently, there are a number of different techniques for analyzing the tagged images. Specifically, k-space-based analysis techniques showed to be much faster than image-based techniques, where harmonic-phase (HARP) and sine-wave modeling (SinMod) stand as two famous techniques of the former group, which are frequently used in clinical studies. In this study, we compared HARP and SinMod and studied inter-observer variability between the two techniques for evaluating myocardial strain and apical-to-base torsion in numerical phantom, nine healthy controls, and thirty diabetic patients...
May 16, 2018: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/29777700/transient-receptor-potential-ankyrin-1-channels-are-involved-in-spontaneous-peptide-hormone-release-from-astrocytes
#2
Mai Takizawa, Kazuki Harada, Kazuaki Nakamura, Takashi Tsuboi
Astrocytes, a large population of glial cells, detect neurotransmitters and respond by increasing intracellular Ca2+ concentration ([Ca2+ ]i ) and releasing chemical molecules called gliotransmitters. Recently discovered Ca2+ influx through transient receptor potential ankyrin 1 (TRPA1) channels is reported to cause spontaneous [Ca2+ ]i increase in astrocytes. While several physiological functions of TRPA1-mediated spontaneous Ca2+ signal have been revealed, relation with gliotransmitter release, especially peptide hormone exocytosis is largely unknown...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777695/proteasome-mediated-protein-degradation-is-enhanced-by-fusion-ubiquitin-with-unstructured-degron
#3
Tomonao Inobe, Masayuki Tsukamoto, Miyuki Nozaki
Methods to induce proteasomal degradation of unwanted proteins are valuable in biomedical studies and thus receive increasing attention. For efficient degradation, the proteasome requires both a ubiquitin tag, which delivers substrates to the proteasome, and an unstructured region, where the proteasome engages the substrate for unfolding and degradation. We fused two degron components into a single molecule to create a fusion protein comprising ubiquitin and Rpn4-derived unstructured region. We demonstrated that the fusion protein retained its function to polyubiquitinate target proteins, thereby inducing more efficient proteasomal target degradation than wild-type ubiquitin in vitro and in cells...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777486/physiological-and-quantitative-proteomic-analyses-unraveling-potassium-deficiency-stress-response-in-alligator-weed-alternanthera-philoxeroides-l-root
#4
Li-Qin Li, Lun Liu, Wei Zhuo, Qian Chen, Sheng Hu, Shuang Peng, Xi-Yao Wang, Yi-Fei Lu, Li-Ming Lu
Physiological and iTRAQ based proteomic analysis provided new insights into potassium deficiency stress response in alligator weed root. Alligator weed (Alternanthera philoxeroides) has a strong ability to adapt to potassium deficiency (LK) stress. Proteomic changes in response to this stress are largely unknown in alligator weed. In this study, we investigated physiological and molecular mechanisms under LK using isobaric tags for relative and absolute quantitation to characterize proteome-level changes in this plant...
May 18, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29776372/curation-of-microarray-oligonucleotides-and-corresponding-ests-cdnas-used-for-gene-expression-analysis-in-zebra-finches
#5
Peter V Lovell, Nicole A Huizinga, Abel Getachew, Brianna Mees, Samantha R Friedrich, Morgan Wirthlin, Claudio V Mello
OBJECTIVES: Zebra finches are a major model organism for investigating mechanisms of vocal learning, a trait that enables spoken language in humans. The development of cDNA collections with expressed sequence tags (ESTs) and microarrays has allowed for extensive molecular characterizations of circuitry underlying vocal learning and production. However, poor database curation can lead to errors in transcriptome and bioinformatics analyses, limiting the impact of these resources. Here we used genomic alignments and synteny analysis for orthology verification to curate and reannotate ~ 35% of the oligonucleotides and corresponding ESTs/cDNAs that make-up Agilent microarrays for gene expression analysis in finches...
May 18, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29776043/reynolds-number-dependent-dynamical-transitions-on-hydrodynamic-synchronization-modes-of-externally-driven-colloids
#6
Norihiro Oyama, Kosuke Teshigawara, John Jairo Molina, Ryoichi Yamamoto, Takashi Taniguchi
The collective dynamics of externally driven N_{p}-colloidal systems (1≤N_{p}≤4) in a confined viscous fluid have been investigated using three-dimensional direct numerical simulations with fully resolved hydrodynamics. The dynamical modes of collective particle motion are studied by changing the particle Reynolds number as determined by the strength of the external driving force and the confining wall distance. For a system with N_{p}=3, we found that at a critical Reynolds number a dynamical mode transition occurs from the doublet-singlet mode to the triplet mode, which has not been reported experimentally...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29775866/identification-of-udp-glucosyltransferases-from-the-aluminum-resistant-tree-eucalyptus-camaldulensis-forming-%C3%AE-glucogallin-the-precursor-of-hydrolyzable-tannins
#7
Ko Tahara, Mitsuru Nishiguchi, Andrej Frolov, Juliane Mittasch, Carsten Milkowski
In the highly aluminum-resistant tree Eucalyptus camaldulensis, hydrolyzable tannins are proposed to play a role in internal detoxification of aluminum, which is a major factor inhibiting plant growth on acid soils. To understand and modulate the molecular mechanisms of aluminum detoxification by hydrolyzable tannins, the biosynthetic genes need to be identified. In this study, we identified and characterized genes encoding UDP-glucose:gallate glucosyltransferase, which catalyzes the formation of 1-O-galloyl-β-d-glucose (β-glucogallin), the precursor of hydrolyzable tannins...
May 15, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29775711/co-expression-purification-and-characterization-of-the-lipase-and-foldase-of-burkholderia-contaminans-lteb11
#8
Robson Carlos Alnoch, Adriano Alves Stefanello, Viviane de Paula Martini, Jeferson Luiz Richter, Cesar Mateo, Emanuel Maltempi de Souza, David Alexander Mitchell, Marcelo Muller-Santos, Nadia Krieger
Genes encoding lipase LipBC (lipA) and foldase LifBC (lipB) were identified in the genome of Burkholderia contaminans LTEB11. Analysis of the predicted amino acid sequence of lipA showed its high identity with lipases from Pseudomonas luteola (91%), Burkholderia cepacia (96%) and Burkholderia lata (97%), and classified LipBC lipase in the lipase subfamily I.2. The genes lipA and lipB were amplified and cloned into expression vectors pET28a(+) and pT7-7, respectively. His-tagged LipBC and native LifBC were co-expressed in Escherichia coli and purified...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775058/multiple-click-selective-trna-synthetases-expand-mammalian-cell-specific-proteomics
#9
Andrew C Yang, Haley du Bois, Niclas Olsson, David Gate, Benoit Lehallier, Daniela Berdnik, Kyle D Brewer, Carolyn R Bertozzi, Joshua E Elias, Tony Wyss-Coray
Bio-orthogonal tools enable cell-type-specific proteomics, a prerequisite to understanding biological processes in multicellular organisms. Here we report two engineered aminoacyl-tRNA synthetases for mammalian bio-orthogonal labeling: a tyrosyl ( ScTyrY34G ) and a phenylalanyl ( MmPheT412G ) tRNA synthetase that incorporate azide-bearing noncanonical amino acids specifically into the nascent proteomes of host cells. Azide-labeled proteins are chemoselectively tagged via azide-alkyne cycloadditions with fluorophores for imaging or affinity resins for mass spectrometric characterization...
May 18, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29774898/chiroptical-methods-in-a-wide-wavelength-range-for-obtaining-ln-3-complexes-with-circularly-polarized-luminescence-of-practical-interest
#10
Marcin Górecki, Luca Carpita, Lorenzo Arrico, Francesco Zinna, Lorenzo Di Bari
We studied enantiopure chiral trivalent lanthanide (Ln3+ = La3+, Sm3+, Eu3+, Gd3+, Tm3+, and Yb3+) complexes with two fluorinated achiral tris(β-diketonate) ligands (HFA = hexafluoroacetylacetonate and TTA = 2-thenoyltrifluoroacetonate), incorporating a chiral bis(oxazolinyl)pyridine (PyBox) unit as a neutral ancillary ligand, by the combined use of optical and chiroptical methods, ranging from UV to IR both in absorption and circular dichroism (CD), and including circularly polarized luminescence (CPL). Ultimately, all the spectroscopic information is integrated into a total and a chiroptical super-spectrum, which allows one to characterize a multidimensional chemical space, spanned by the different Ln3+ ions, the acidity and steric demand of the diketone and the chirality of the PyBox ligand...
May 18, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29774466/rs2227982-and-rs2227981-in-pdcd1-gene-are-functional-snps-associated-with-t1d-risk-in-east-asian
#11
Yong Gu, Lei Xiao, Wei Gu, Shu Chen, Yingjie Feng, Jian Wang, Zhixiao Wang, Yun Cai, Heng Chen, Xinyu Xu, Yun Shi, Mei Zhang, Kuanfeng Xu, Tao Yang
AIMS: To investigate whether PDCD1 gene polymorphisms are functional, and their associations with T1D risk and related clinical characteristics. METHODS: A total of 3060 Chinese Han individuals (1019 T1D patients and 2041 healthy controls) were genotyped for 4 tag single nucleotide polymorphisms (SNPs) within the PDCD1 region (rs2227982, rs7421861, rs10204525, and rs6710479) and another most studied synonymous SNP, rs2227981. In addition, 251 healthy individuals underwent an oral glucose tolerance test (OGTT); measures of insulin release and sensitivity were estimated from insulinogenic, BIGTT, Matsuda...
May 17, 2018: Acta Diabetologica
https://www.readbyqxmd.com/read/29774148/genetic-variations-in-the-tlr3-locus-are-associated-with-eosinophilic-esophagitis
#12
Robledo Ávila-Castellano, José-Raúl García-Lozano, Stefan Cimbollek, Alfredo J Lucendo, Juan-Manuel Bozada, Joaquín Quiralte
Background: Eosinophilic esophagitis (EoE) is an antigen-driven disease mediated by an abnormal immune Th2 response. Objective: The objective of this article is to investigate genes associated with regulating immune responses leading to disease susceptibility. Methods: Twenty-seven tag single nucleotide polymorphisms (tSNPs) selected in five candidate genes ( TLR3, TLR4, FOXP3, FLG and TSLP ) were genotyped in 218 EoE patients and 376 controls...
April 2018: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/29773902/nedd9-promotes-oncogenic-signaling-a-stem-mesenchymal-gene-signature-and-aggressive-ovarian-cancer-growth-in-mice
#13
Rashid Gabbasov, Fang Xiao, Caitlin G Howe, Laura E Bickel, Shane W O'Brien, Daniel Benrubi, Thuy-Vy Do, Yan Zhou, Emmanuelle Nicolas, Kathy Q Cai, Samuel Litwin, Sachiko Seo, Erica A Golemis, Denise C Connolly
Neural precursor cell expressed, developmentally downregulated 9 (NEDD9) supports oncogenic signaling in a number of solid and hematologic tumors. Little is known about the role of NEDD9 in ovarian carcinoma (OC), but available data suggest elevated mRNA and protein expression in advanced stage high-grade cancers. We used a transgenic MISIIR-TAg mouse OC model combined with genetic ablation of Nedd9 to investigate its action in the development and progression of OC. A Nedd9-/- genotype delayed tumor growth rate, reduced incidence of ascites, and reduced expression and activation of signaling proteins including SRC, STAT3, E-cadherin, and AURKA...
May 18, 2018: Oncogene
https://www.readbyqxmd.com/read/29772416/red-fluorescent-carbon-dots-with-phenylboronic-acid-tags-for-quick-detection-of-fe-iii-in-pc12-cells
#14
Changjian Li, Yueqi Wang, Xiaojie Zhang, Xueling Guo, Xiaoxuan Kang, Libo Du, Yang Liu
Carbon dots have attracted considerable attention in the field of biosensors and bioimaging because of their excellent optical performance and low toxicity. However, the cellular uptake of the reported carbon dots generally has a low efficiency, which limits their practical applications. In this study, we reported a novel red fluorescent sulfur and nitrogen co-doped carbon dots with small molecular phenylboronic acid tags (i.e. S, N-CDs-PBA). The S, N-CDs-PBA can be taken up rapidly by PC12 cells in twenty minutes and showed high sensitivity for the detection of Fe3+ ions...
May 9, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29772383/a-signal-decreased-electrochemical-immunosensor-for-the-sensitive-detection-of-lag-3-protein-based-on-a-hollow-nanobox-mofs-aupt-alloy
#15
Wei Xu, Zhen Qin, Yutong Hao, Qiang He, Shuai Chen, Zhongshuang Zhang, Dan Peng, Haiyan Wen, Jun Chen, Jingfu Qiu, Chaorui Li
In this work, hollow nanobox metal-organic framework (HNM) nanocomposites were synthesised and utilised for the first time in a signal decreased electrochemical immunosensor for the ultrasensitive quantitative determination of lymphocyte activation gene-3 (LAG-3) protein, which is a newly discovered biomarker. With the aid of signal materials, namely, SiO2 -tagged anti-LAG-3 antibody (SiO2 -Ab2 ) and the biotin-streptavidin system, the sensor can achieve signal amplification. Encapsulation of tin dioxide-functionalised reduced graphene oxide (rGO-SnO2 ) and gold and platinum alloys (AuPt alloys) onto the surface of hollow nanobox metal-organic frameworks (MOFs) was performed to prepare rGO-SnO2 /hollow nanobox-MOFs/AuPt alloys (rGO-SnO2 /HNMs/AuPt) as the matrix...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29772374/evolution-expression-and-characterisation-of-liver-expressed-antimicrobial-peptide-genes-in-ancient-chondrostean-sturgeons
#16
Shuhuan Zhang, Qiaoqing Xu, Hao Du, Zhitao Qi, Youshen Li, Jun Huang, Jun Di, Qiwei Wei
Liver-expressed antimicrobial peptide 2 (leap-2) is an evolutionarily ancient molecule that acts as the key component in vertebrate innate immunity against invading pathogens. Leap-2 has been identified and characterised in several teleosts, but not yet in chondrosteans. Herein, the complete coding sequences of leap-2b and leap-2c were identified from expressed sequence tags (ESTs) isolated from Dabry's sturgeon (Acipenser dabryanus) and Chinese sturgeon (A. sinensis), designated as adleap-2b, adleap-2c, asleap-2b, and asleap-2c, respectively...
May 14, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29772060/nucleocytoplasmic-shuttling-the-ins-and-outs-of-quantitative-imaging
#17
Chris Molenaar, Kate L Weeks
Nucleocytoplasmic protein shuttling is integral to the transmission of signals between the nucleus and the cytoplasm. The nuclear/cytoplasmic distribution of proteins of interest can be determined via fluorescence microscopy, following labelling of the target protein with fluorophore-conjugated antibodies (immunofluorescence) or by tagging the target protein with an autofluorescent protein, such as green fluorescent protein (GFP). The latter enables live cell imaging, a powerful approach that precludes many of the artefacts associated with indirect immunofluorescence in fixed cells...
May 17, 2018: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29771991/progenitor-renin-lineage-cells-are-not-involved-in-the-regeneration-of-glomerular-endothelial-cells-during-experimental-renal-thrombotic-microangiopathy
#18
Leo Ruhnke, Jan Sradnick, Moath Al-Mekhlafi, Michael Gerlach, Florian Gembardt, Bernd Hohenstein, Vladimir T Todorov, Christian Hugo
Endothelial cells (EC) frequently undergo primary or secondary injury during kidney disease such as thrombotic microangiopathy or glomerulonephritis. Renin Lineage Cells (RLCs) serve as a progenitor cell niche after glomerular damage in the adult kidney. However, it is not clear whether RLCs also contribute to endothelial replenishment in the glomerulus following endothelial injury. Therefore, we investigated the role of RLCs as a potential progenitor niche for glomerular endothelial regeneration. We used an inducible tet-on triple-transgenic reporter strain mRen-rtTAm2/LC1/LacZ to pulse-label the renin-producing RLCs in adult mice...
2018: PloS One
https://www.readbyqxmd.com/read/29771517/spectroscopy-of-proton-coordination-with-ethylenediamine
#19
J Philipp Wagner, David C McDonald Ii, Michael A Duncan
Protonated ethylenediamine monomer, dimer and trimer were produced in the gas phase by an electrical discharge/supersonic expansion of argon seeded with ethylenediamine (C2H8N2, en) vapor. Infrared spectra of these ions were measured in the region from 1000 to 4000 cm-1 using laser photodissociation and argon tagging. Computations at the CBS-QB3 level were performed to explore possible isomers and understand the infrared spectra. The protonated monomer exhibits a gauche conformation and an intramolecular hydrogen bond...
May 17, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29771294/the-maize-lilliputian1-lil1-gene-encoding-a-brassinosteroid-cytochrome-p450-c-6-oxidase-is-involved-in-plant-growth-and-drought-response
#20
Giulia Castorina, Martina Persico, Massimo Zilio, Stefano Sangiorgio, Laura Carabelli, Gabriella Consonni
Background and Aims: Brassinosteroids (BRs) are plant hormones involved in many developmental processes as well as in plant-environment interactions. Their role was investigated in this study through the analysis of lilliputian1-1 (lil1-1), a dwarf mutant impaired in BR biosynthesis in maize (Zea mays). Methods: We isolated lil1-1 through transposon tagging in maize. The action of lil1 was investigated through morphological and genetic analysis. Moreover, by comparing lil1-1 mutant and wild-type individuals grown under drought stress, the effect of BR reduction on the response to drought stress was examined...
May 16, 2018: Annals of Botany
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