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https://www.readbyqxmd.com/read/28531877/folate-n-acetyl-glucosamine-conjugated-mesoporous-silica-nanoparticles-for-targeting-breast-cancer-cells-a-comparative-study
#1
Pramod Kumar, Prajakta Tambe, Kishore M Paknikar, Virendra Gajbhiye
Folate receptors (FR) have been well recognized as a marker to target nano-sized carriers for cancer diagnosis and therapy. In contrast, influx transport systems (e.g. GLUT transporters) that transport essential amino acids and nutrients to cancer cells have not been exploited much for targeted delivery. In this study, folic acid- or n-acetyl glucosamine- functionalized mesoporous silica nanoparticles loaded with doxorubicin (DOX-FA-MSNPs or DOX-NAG-MSNPs) were prepared, characterized and compared for targeting along with cytotoxicity towards MCF-7 and MDA-MB-231 human breast cancer cells...
May 11, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28530747/bioorthogonal-double-fluorogenic-siliconrhodamine-probes-for-intracellular-super-resolution-microscopy
#2
E Kozma, G Estrada Girona, G Paci, E A Lemke, P Kele
A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of genetically manipulated intracellular proteins and subsequent imaging using super-resolution microscopy.
May 22, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28530680/the-effect-of-glucose-dependent-insulinotropic-polypeptide-gip-variants-on-visceral-fat-accumulation-in-han-chinese-populations
#3
T Wang, X Ma, T Tang, K Higuchi, D Peng, R Zhang, M Chen, J Yan, S Wang, D Yan, Z He, F Jiang, Y Bao, W Jia, K Ishida, C Hu
OBJECTIVES: We aim to validate the effects of glucose-dependent insulinotropic polypeptide (GIP) on fat distribution and glucose metabolism in Han Chinese populations. METHODS: We genotyped six tag single-nucleotide polymorphisms (SNPs) of GIP and four tag SNPs of glucose-dependent insulinotropic polypeptide receptor (GIPR) among 2884 community-based individuals from Han Chinese populations. Linear analysis was applied to test the associations of these variants with visceral fat area (VFA) and subcutaneous fat area (SFA) quantified by magnetic resonance imaging as well as glucose-related traits...
May 22, 2017: Nutrition & Diabetes
https://www.readbyqxmd.com/read/28530595/comparison-of-solvents-for-extraction-of-krill-oil-from-krill-meal-lipid-yield-phospholipids-content-fatty-acids-composition-and-minor-components
#4
Dan Xie, Jun Jin, Jiang Sun, Li Liang, Xiaosan Wang, Wei Zhang, Xingguo Wang, Qingzhe Jin
The effects of seven different extraction solvents (ethanol, isopropanol, acetone, ethyl acetate, isohexane, n-hexane, and subcritical butane) on the lipid yield and quality of the oil extracted from krill meal were investigated in this study. Phospholipids (PL), fatty acids (FA) composition and minor components including sterols, astaxanthin, vitamin A and tocopherols in the extracted krill oil were analyzed. The results indicated that ethanol and isopropanol led to comparatively higher lipid yields (16.33 and 14...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530584/triacylglycerol-regioisomers-in-human-milk-resolved-with-an-algorithmic-novel-electrospray-ionization-tandem-mass-spectrometry-method
#5
Heikki Kallio, Matts Nylund, Pontus Boström, Baoru Yang
A highly sensitive mass spectrometric (MS) method was developed and validated to analyze ratios of regioisomeric triacylglycerols (TAGs) in fats and oils. UPLC resolution of lithiated TAGs followed by daughter scan MS/MS of positive ions revealed several indicative ions for quantitative analysis. Reference TAGs containing C14-C20 fatty acids (FAs) showed good linear response. Analysis of Finnish and Chinese pooled human milk samples revealed hundreds of regioisomeric TAGs. At least 64mol% of the TAGs were quantified with relative standard deviation <17%...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530298/aptabody-aptatope-interactions-in-aptablotting-assays
#6
Simranjeet Singh Sekhon, Hyun-Ju Um, Woo-Ri Shin, Sang-Hee Lee, Jiho Min, Ji-Young Ahn, Yang-Hoon Kim
We demonstrate an aptablotting assay method that involves direct and indirect aptabody recognition. Nanoscale single-stranded DNA aptamers against GST and DIG-tags are utilized as aptabodies (GST-2 and DIG-1, respectively), and the GST-2 aptabody binding site, or aptatope, as predicted by a MOE-docking simulation of the protein-aptamer complex, shows the interaction of the GST-2 aptabody at the catalytically active region. The aptabody-aptatope interaction was evaluated by an in vitro enzyme inhibitory analysis...
May 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/28529834/development-and-characterization-of-est-ssr-markers-for-begonia-luzhaiensis-begoniaceae
#7
Yu-Hsin Tseng, Han-Yau Huang, Wei-Bin Xu, Hsun-An Yang, Yan Liu, Ching-I Peng, Kuo-Fang Chung
PREMISE OF THE STUDY: Microsatellite primers were developed for Begonia luzhaiensis (Begoniaceae) to assess genetic diversity and population genetic structure. METHODS AND RESULTS: Based on the transcriptome data of B. luzhaiensis, 60 primer pairs were selected for initial validation, of which 16 yielded polymorphic microsatellite loci in 57 individuals. The number of alleles observed for these 16 loci ranged from one to nine. The observed and expected heterozygosity ranged from 0...
May 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28529717/gene-length-and-detection-bias-in-single-cell-rna-sequencing-protocols
#8
Belinda Phipson, Luke Zappia, Alicia Oshlack
Background: Single cell RNA sequencing (scRNA-seq) has rapidly gained popularity for profiling transcriptomes of hundreds to thousands of single cells. This technology has led to the discovery of novel cell types and revealed insights into the development of complex tissues. However, many technical challenges need to be overcome during data generation. Due to minute amounts of starting material, samples undergo extensive amplification, increasing technical variability. A solution for mitigating amplification biases is to include unique molecular identifiers (UMIs), which tag individual molecules...
2017: F1000Research
https://www.readbyqxmd.com/read/28528707/molecular-transcriptional-and-functional-insights-into-duplicated-goose-type-lysozymes-from-sebastes-schlegelii-and-their-potential-immunological-role
#9
Jehanathan Nilojan, S D N K Bathige, Roopasingam Kugapreethan, W S Thulasitha, Bo-Hye Nam, Jehee Lee
Black rockfish (Sebastes schlegelii), an important aquaculture species in Korea, has been affected by bacterial diseases leading to a drastic decline in production. Goose-type lysozyme (LysG) is a key enzyme of the innate immune system to eradicate bacterial infections. In this study, two isoforms of LysG from black rockfish, designated as RfLysG1 and RfLysG2, have been identified and characterized at the molecular, transcriptional, and functional levels. The deduced amino acid sequences had the LysG family characteristics and exhibited conserved properties, including active residues and domains...
May 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28528635/relocation-is-the-key-to-successful-correlative-fluorescence-and-scanning-electron-microscopy
#10
Delfine Cheng, Gerald Shami, Marco Morsch, Minh Huynh, Patrick Trimby, Filip Braet
In this chapter the authors report on an automated hardware and software solution enabling swift correlative sample array mapping of fluorescently stained molecules within cells and tissues across length scales. Samples are first observed utilizing wide-field optical and fluorescence microscopy, followed by scanning electron microscopy, using calibration points on a dedicated sample-relocation holder. We investigated HeLa cells in vitro, fluorescently labeled for monosialoganglioside one (GM-1), across both imaging platforms within tens of minutes of initial sample preparation...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528631/a-fully-integrated-three-dimensional-fluorescence-to-electron-microscopy-correlative-workflow
#11
Claudia S López, Cedric Bouchet-Marquis, Christopher P Arthur, Jessica L Riesterer, Gregor Heiss, Guillaume Thibault, Lee Pullan, Sunjong Kwon, Joe W Gray
While fluorescence microscopy provides tools for highly specific labeling and sensitive detection, its resolution limit and lack of general contrast has hindered studies of cellular structure and protein localization. Recent advances in correlative light and electron microscopy (CLEM), including the fully integrated CLEM workflow instrument, the FEI CorrSight with MAPS, have allowed for a more reliable, reproducible, and quicker approach to correlate three-dimensional time-lapse confocal fluorescence data, with three-dimensional focused ion beam-scanning electron microscopy data...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528629/correlative-light-and-electron-microscopic-detection-of-gfp-labeled-proteins-using-modular-apex
#12
Nicholas Ariotti, Thomas E Hall, Robert G Parton
The use of green fluorescent protein (GFP) and related proteins has revolutionized light microscopy. Here we describe a rapid and simple method to localize GFP-tagged proteins in cells and in tissues by electron microscopy (EM) using a modular approach involving a small GFP-binding peptide (GBP) fused to the ascorbate peroxidase-derived APEX2 tag. We provide a method for visualizing GFP-tagged proteins by light and EM in cultured cells and in the zebrafish using modular APEX-GBP. Furthermore, we describe in detail the benefits of this technique over many of the currently available correlative light and electron microscopy approaches and demonstrate APEX-GBP is readily applicable to modern three-dimensional techniques...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528593/polymannuronic-acid-ameliorated-obesity-and-inflammation-associated-with-a-high-fat-and-high-sucrose-diet-by-modulating-the-gut-microbiome-in-a-murine-model
#13
Fang Liu, Xiong Wang, Hongjie Shi, Yuming Wang, Changhu Xue, Qing-Juan Tang
Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities. In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice. A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0). PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure...
May 22, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28528198/on-national-flags-and-language-tags-effects-of-flag-language-congruency-in-bilingual-word-recognition
#14
Jonathan Grainger, Mathieu Declerck, Yousri Marzouki
French-English bilinguals performed a generalized lexical decision experiment with mixed lists of French and English words and pseudo-words. In Experiment 1, each word/pseudo-word was superimposed on the picture of the French or UK flag, and flag-word congruency was manipulated. The flag was not informative with respect to either the lexical decision response or the language of the word. Nevertheless, lexical decisions to word stimuli were faster following the congruent flag compared with the incongruent flag, but only for French (L1) words...
May 18, 2017: Acta Psychologica
https://www.readbyqxmd.com/read/28528164/mri-derived-myocardial-strain-measures-in-normal-subjects
#15
Ha Q Vo, Thomas H Marwick, Kazuaki Negishi
OBJECTIVES: The aim of this study was to perform a systematic review and meta-analysis to estimate the normal ranges of magnetic resonance imaging (MRI)-based feature tracking (FT) and to identify sources of variations. Similar analyses were also performed for strain encoding, displacement encoding with stimulated echoes, and myocardial tagging. BACKGROUND: MRI-FT is a novel technique for quantification of myocardial deformation using MRI cine images. However, the reported 95% confidence intervals (CIs) from the 2 largest studies have no overlaps...
May 11, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28527812/adhesive-interfacial-characteristics-and-the-related-bonding-performance-of-four-self-etching-adhesives-with-different-functional-monomers-applied-to-dentin
#16
Ruirui Wang, Yilin Shi, Tingting Li, Yueping Pan, Yu Cui, Wenwei Xia
OBJECTIVES: To examine the interfacial chemical and morphological characteristics of four self-etching adhesives bonded to dentin with different functional monomers. Further, to evaluate the effects of this interaction between functional monomers and dentin on short-term in vitro bonding performance of the four adhesives. METHODS: Clearfil SE Bond (CSE) and Scotchbond Universal (SU) containing 10-methacryloxydecyl dihydrogen phosphate (10-MDP), Optibond XTR (OX) containing glycero-phosphate dimethacrylate (GPDM), and Adper Easy One (AEO) containing 6-methacryloyloxyhexyl dihydrogen phosphate (6-MHP) were applied to the dentin surface according to the instructions supplied with each...
May 17, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28527624/understanding-aerobic-anaerobic-metabolism-in-caldibacillus-debilis-through-a-comparison-with-model-organisms
#17
Scott Wushke, Victor Spicer, Xiang Li Zhang, Brian Fristensky, Oleg V Krokhin, David B Levin, Nazim Cicek, Richard Sparling
Caldibacillus debilis GB1 is a facultative anaerobe isolated from a thermophilic aero-tolerant cellulolytic enrichment culture. There is a lack of representative proteomes of facultative anaerobic thermophilic Bacillaceae, exploring aerobic/anaerobic expression. The C. debilis GB1 genome was sequenced and annotated, and the proteome characterized under aerobic and anaerobic conditions while grown on cellobiose. The draft sequence of C. debilis GB1 contains a 3,340,752 bp chromosome and a 5,386 bp plasmid distributed over 49 contigs...
April 13, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28527213/sorl1-variants-show-different-association-with-early-onset-and-late-onset-alzheimer-s-disease-risk
#18
Guiyou Liu, Jing-Yi Sun, Meiling Xu, Xiao-Yi Yang, Bao-Liang Sun
A recent study sequenced the full coding region of SORL1 in 1,255 early-onset Alzheimer's disease (EOAD) cases and 1,938 control individuals, and investigated the contribution of genetic variability in SORL1 to EOAD risk in a European cohort. This study identified six common variants and five low frequency variants in the SORL1 coding sequence. However, none of these 11 variants was significantly associated with EOAD risk after adjusting for multiple testing. We consider whether these 11 SORL1 variants identified in European EOAD contribute to late-onset Alzheimer's disease (LOAD) risk in individuals of European ancestry...
May 17, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28527112/inhalation-of-roman-chamomile-essential-oil-attenuates-depressive-like-behaviors-in-wistar-kyoto-rats
#19
Yingying Kong, Ting Wang, Rong Wang, Yichuan Ma, Shanshan Song, Juan Liu, Weiwei Hu, Shengtian Li
The idea of aromatherapy, using essential oils, has been considered as an alternative antidepressant treatment. In the present study, we investigated the effect of Roman chamomile essential oil inhalation for two weeks on depressive-like behaviors in Wistar-Kyoto (WKY) rats. We found that inhalation of either Roman chamomile or one of its main components α-pinene, attenuated depressive-like behavior in WKY rats in the forced swim test. Using isobaric tags for relative and absolute quantitation analysis (iTRAQ), we found that inhalation of α-pinene increased expression of proteins that are involved in oxidative phosphorylation, such as cytochrome c oxidase subunit 6C-2, cytochrome c oxidase subunit 7A2, ATPase inhibitor in the hippocampus, and cytochrome c oxidase subunit 6C-2, ATP synthase subunit e, Acyl carrier protein, and Cytochrome b-c1 complex subunit 6 in the PFC (prefrontal cortex)...
May 16, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28526614/human-aldehyde-dehydrogenase-3a1-aldh3a1-exhibits-chaperone-like-function
#20
Georgia-Persephoni Voulgaridou, Ilias Tsochantaridis, Theodora Mantso, Rodrigo Franco, Mihalis I Panayiotidis, Aglaia Pappa
Aldehyde dehydrogenase 3A1 (ALDH3A1) is a metabolic enzyme that catalyzes the oxidation of various aldehydes. Certain types of epithelial tissues in mammals, especially those continually exposed to environmental stress (e.g., corneal epithelium), express ALDH3A1 at high levels and its abundance in such tissues is perceived to help to maintain cellular homeostasis under conditions of oxidative stress. Metabolic as well as non-metabolic roles for ALDH3A1 have been associated with its mediated resistance to cellular oxidative stress...
May 16, 2017: International Journal of Biochemistry & Cell Biology
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