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https://www.readbyqxmd.com/read/29675001/-leishmania-infantum-lipophosphoglycan-deficient-mutants-a-tool-to-study-host-cell-parasite-interplay
#1
Milena Lázaro-Souza, Christine Matte, Jonilson B Lima, Guillermo Arango Duque, Graziele Quintela-Carvalho, Áislan de Carvalho Vivarini, Sara Moura-Pontes, Cláudio P Figueira, Flávio H Jesus-Santos, Ulisses Gazos Lopes, Leonardo P Farias, Théo Araújo-Santos, Albert Descoteaux, Valéria M Borges
Lipophosphoglycan (LPG) is the major surface glycoconjugate of metacyclic Leishmania promastigotes and is associated with virulence in various species of this parasite. Here, we generated a LPG-deficient mutant of Leishmania infantum , the foremost etiologic agent of visceral leishmaniasis in Brazil. The L. infantum LPG-deficient mutant (Δ lpg1 ) was obtained by homologous recombination and complemented via episomal expression of LPG1 (Δ lpg1 + LPG1 ). Deletion of LPG1 had no observable effect on parasite morphology or on the presence of subcellular organelles, such as lipid droplets...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29670879/human-adipose-derived-mesenchymal-stem-stromal-cells-handling-protocols-lipid-droplets-and-proteins-double-staining
#2
Aldana D Gojanovich, María C Gimenez, Diego Masone, Tania M Rodriguez, Ricardo A Dewey, Laura R Delgui, Diego M Bustos, Marina Uhart
Human Adipose-derived mesenchymal stem/stromal cells (hASCs) are of great interest because of their potential for therapeutic approaches. The method described here covers every single step necessary for hASCs isolation from subcutaneous abdominal adipose tissue, multicolor phenotyping by flow cytometry, and quantitative determination of adipogenic differentiation status by means of lipid droplets (LDs) accumulation, and Western blot analysis. Moreover, to simultaneously analyze both LDs accumulation and cellular proteins localization by fluorescence microscopy, we combined Oil Red O (ORO) staining with immunofluorescence detection...
2018: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/29670150/visualizing-multiple-inter-organelle-contact-sites-using-the-organelle-targeted-split-gfp-system
#3
Yuriko Kakimoto, Shinya Tashiro, Rieko Kojima, Yuki Morozumi, Toshiya Endo, Yasushi Tamura
Functional integrity of eukaryotic organelles relies on direct physical contacts between distinct organelles. However, the entity of organelle-tethering factors is not well understood due to lack of means to analyze inter-organelle interactions in living cells. Here we evaluate the split-GFP system for visualizing organelle contact sites in vivo and show its advantages and disadvantages. We observed punctate GFP signals from the split-GFP fragments targeted to any pairs of organelles among the ER, mitochondria, peroxisomes, vacuole and lipid droplets in yeast cells, which suggests that these organelles form contact sites with multiple organelles simultaneously although it is difficult to rule out the possibilities that these organelle contacts sites are artificially formed by the irreversible associations of the split-GFP probes...
April 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29670081/seipin-deficiency-in-mice-causes-loss-of-dopaminergic-neurons-via-aggregation-and-phosphorylation-of-%C3%AE-synuclein-and-neuroinflammation
#4
Ling Wang, Juan Hong, Yajuan Wu, George Liu, Wenfeng Yu, Ling Chen
Seipin gene is originally found in type 2 congenital generalized lipodystrophy (CGL2) to involve lipid droplet formation. Recently, decrease of seipin expression is reported in substantia nigra of Parkinson's disease patients. Dopaminergic neurons in substantia nigra pars compacta expressed the seipin protein. The objective of this study is to investigate influence of the seipin deficiency on dopaminergic neurons and motor behaviors. Neuronal seipin knockout (seipin-nKO) mice (3-12 months of age) displayed an age-related deficit in motor coordination...
April 18, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29669827/osteopontin-regulates-hcv-replication-and-assembly-via-interaction-with-hcv-proteins-and-lipid-droplets-and-by-binding-to-receptors-%C3%AE-v%C3%AE-3-and-cd44
#5
Jawed Iqbal, Mehuli Sarkar-Dutta, Steven McRae, Akshaya Ramachandran, Binod Kumar, Gulam Waris
Hepatitis C virus (HCV) replication and assembly occur at the specialized site of endoplasmic reticulum (ER) membranes and lipid droplets (LDs), respectively. Recently, several host proteins have been shown to be involved in HCV replication and assembly. In the present study, we demonstrate the important relationship among osteopontin (OPN), ER and LDs. OPN is a secreted phosphoprotein and overexpression of OPN in hepatocellular carcinoma (HCC) tissue can lead to invasion and metastasis. OPN expression is also enhanced in HCV-associated HCC...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29668854/prostate-cancer-chemoprevention-by-sulforaphane-in-a-preclinical-mouse-model-is-associated-with-inhibition-of-fatty-acid-metabolism
#6
Krishna B Singh, Su-Hyeong Kim, Eun-Ryeong Hahm, Subrata K Pore, Bruce L Jacobs, Shivendra V Singh
Increased de novo synthesis of fatty acids is a rather unique and targetable mechanism of human prostate cancer. We have shown previously that oral administration of sulforaphane (SFN) significantly inhibits the incidence and/or burden of prostatic intraepithelial neoplasia and well-differentiated adenocarcinoma in TRansgenic Adenocarcinoma of Mouse Prostate (TRAMP) mice. The present study used cellular models of prostate cancer and archived plasma/adenocarcinoma tissues and sections from the TRAMP study to demonstrate inhibition of fatty acid synthesis by SFN treatment in vitro and in vivo...
April 13, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29667316/opposite-effects-of-the-spinach-food-matrix-on-lutein-bioaccessibility-and-intestinal-uptake-lead-to-unchanged-bioavailability-compared-to-pure-lutein
#7
Marielle Margier, Caroline Buffière, Pascale Goupy, Didier Remond, Charlotte Halimi, Catherine Caris, Patrick Borel, Emmanuelle Reboul
SCOPE: Food matrix is generally believed to alter carotenoid bioavailability, but its effect on xanthophylls is usually limited. We thus aimed to decipher the digestion-absorption process of lutein in the presence or not of a food matrix. METHODS: We assessed lutein transfer to gastric-like lipid droplets or artificial mixed micelles when lutein was added to test meals either as a pure molecule ((all-E)-lutein) or in canned spinach ((Z) + (all-E)-lutein). The obtained mixed micelles were delivered to Caco-2 cells to evaluate lutein uptake...
April 17, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29665934/-effect-of-regulating-ppar%C3%AE-by-mtor-signaling-on-adipogenesis-of-bone-marrow-mesenchymal-stem-cells-from-aplastic-anemia
#8
Fang Wei, Wei-Huan Zhang, Xiu-Lian Zhang, Xiao-Jing Ge, Shu-Qin Zhang
OBJECTIVE: To study the effect and mechanism of mTOR signaling on adipogenesis of bone marrow mesenchymal stem cells(BM-MSCs) from aplastic anemia (AA) patients through regulation of PPARγ. METHODS: BM-MSCs were isolated from 24 newly diagnosed AA patients and 24 healthy controls. The surface antigen expression of BM-MSCs was identified by flow cytometry. The capacity of adipogenic differentiation of BM-MSCs was determined by lipid droplets based on Oil Red O staining and by the expression of FABP4 based on Western blot...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29663555/dehydroleucodine-inhibits-mitotic-clonal-expansion-during-adipogenesis-through-cell-cycle-arrest
#9
S Abood, M L Veisaga, L A López, M A Barbieri
Aberrant levels of preadipocyte differentiation, triggered by adipocyte hyperplasia and hypertrophy, results in the obesogenic phenotype. Obesity is a risk factor for several metabolic disorders. In this paper, dehydroleucodine inhibited the accumulation of lipid droplets and decreased the elevations of triglycerides, and this inhibitory effect occurred during the early stage of adipogenesis. Thus, not only did dehydroleucodine downregulate the expression of C/EBPα and PPARγ, it also strongly blocked the expression of C/EBPβ, an early stage biomarker of early adipogenesis, in a concentration-dependent manner...
April 16, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29663061/sizing-lipid-droplets-from-adult-and-geriatric-mouse-liver-tissue-via-nanoparticle-tracking-analysis
#10
Katherine A Muratore, Charles P Najt, Nicholas M Livezey, James Marti, Douglas G Mashek, Edgar A Arriaga
The significance of lipid droplets in lipid metabolism, cell signaling, and regulating longevity is increasingly recognized, yet the lipid droplet's unique properties and architecture make it difficult to size and study using conventional methods. To begin to address this issue, we demonstrate the capabilities of nanoparticle tracking analysis (NTA) for sizing of lipid droplets. NTA was found to be adequate to assess lipid droplet stability over time, indicating that lipid droplet preparations are stable for up to 24 h...
April 17, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29662634/caloric-restriction-delays-yeast-chronological-aging-by-remodeling-carbohydrate-and-lipid-metabolism-altering-peroxisomal-and-mitochondrial-functionalities-and-postponing-the-onsets-of-apoptotic-and-liponecrotic-modes-of-regulated-cell-death
#11
Anthony Arlia-Ciommo, Anna Leonov, Adam Beach, Vincent R Richard, Simon D Bourque, Michelle T Burstein, Pavlo Kyryakov, Alejandra Gomez-Perez, Olivia Koupaki, Rachel Feldman, Vladimir I Titorenko
A dietary regimen of caloric restriction delays aging in evolutionarily distant eukaryotes, including the budding yeast Saccharomyces cerevisiae . Here, we assessed how caloric restriction influences morphological, biochemical and cell biological properties of chronologically aging yeast advancing through different stages of the aging process. Our findings revealed that this low-calorie diet slows yeast chronological aging by mechanisms that coordinate the spatiotemporal dynamics of various cellular processes before entry into a non-proliferative state and after such entry...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29660846/phospholipase-d2-promotes-disease-progression-of-renal-cell-carcinoma-through-the-induction-of-angiogenin
#12
Shuya Kandori, Takahiro Kojima, Taeko Matsuoka, Takayuki Yoshino, Aiko Sugiyama, Eijiro Nakamura, Toru Shimazui, Yuji Funakoshi, Yasunori Kanaho, Hiroyuki Nishiyama
A hallmark of clear cell renal cell carcinoma (ccRCC) is the presence of intracellular lipid droplets (LD) and it is assumed that phosphatidic acid (PA) produced by phospholipase D (PLD) plays some roles in the LD formation. However, little is known about the significance of PLD in ccRCC. In this study, we examined the expression levels of PLD in ccRCC. The classical mammalian isoforms of PLD are PLD1 and PLD2, and the levels of both mRNAs were higher at the primary tumor sites than in normal kidney tissues...
April 16, 2018: Cancer Science
https://www.readbyqxmd.com/read/29656500/mitochondria-glycogen-and-lipid-droplets-in-skeletal-muscle-during-testosterone-treatment-and-strength-training-a-randomized-double-blinded-placebo-controlled-trial
#13
R C Jensen, L L Christensen, J Nielsen, H D Schrøder, T Kvorning, K Gejl, K Højlund, D Glintborg, M Andersen
Low testosterone levels in aging men are associated with insulin resistance. Mitochondrial dysfunction, changes in glycogen metabolism, and lipid accumulation are linked to insulin resistance in skeletal muscle. In this randomized, double-blinded, placebo-controlled study, we investigated the effects of six-month testosterone replacement therapy (TRT) and strength training (ST) on mitochondrial, glycogen, and lipid droplet (LD) content in skeletal muscle of aging men with subnormal bioavailable testosterone (BioT) levels...
April 15, 2018: Andrology
https://www.readbyqxmd.com/read/29653099/herbacetin-a-flaxseed-flavonoid-ameliorates-high-percent-dietary-fat-induced-insulin-resistance-and-lipid-accumulation-through-the-regulation-of-hepatic-lipid-metabolizing-and-lipid-regulating-enzymes
#14
Chinnadurai Veeramani, Mohammed A Alsaif, Khalid S Al-Numair
Healthy plants and their constituents have been considered as a safe remedy for the treatment of obesity and obesity associated diseases. Herbacetin is a dietary flavonoid that has been explored for many pharmacological activities; but, the anti-hyperglycaemic and anti-hyperlipidemic properties of herbacetin have not yet been explored. The present study was performed to evaluate the ameliorative effect of herbacetin on high-fat diet-induced hyperglycaemia and hyperlipidemia in 57BL/6 J mice. Obesity associated insulin resistance was induced by continuously feeding the mice with high-fat diet for 10 weeks...
April 10, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29650752/lipid-transfer-proteins-in-the-assembly-of-apob-containing-lipoproteins
#15
Alaa Sirwi, M Mahmood Hussain
A better understanding of intracellular lipoprotein assembly may help identify proteins with important roles in lipid disorders. ApoB-containing lipoproteins are macromolecular lipid and protein micelles that act as specialized transport vehicles for hydrophobic lipids. They are assembled predominantly in enterocytes and hepatocytes to transport dietary and endogenous fat, respectively, to different tissues. Assembly occurs in the endoplasmic reticulum and is dependent on lipid re-synthesis in the endoplasmic reticulum and on a chaperone, namely microsomal triglyceride transfer protein...
April 12, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29648824/impact-of-interfacial-composition-on-lipid-and-protein-co-oxidation-in-oil-in-water-emulsions-containing-mixed-emulisifers
#16
Zhenbao Zhu, Cui Zhao, Jianhua Yi, Ning Liu, Yungang Cao, Eric A Decker, David Julian McClements
The impact of interfacial composition on lipid and protein co-oxidation in oil-in-water emulsions containing a mixture of proteins and surfactants was investigated. The emulsions consisted of 5% v/v walnut oil, 0.5% w/v whey protein isolate (WPI), and 0 to 0.4% w/v Tween 20 (pH 3 and 7). The protein surface load, magnitude of the ξ-potential, and mean particle diameter of the emulsions decreased as the Tween 20 concentration was increased, indicating the whey proteins were displaced by this non-ionic surfactant...
April 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29643990/systematic-characterization-of-autophagy-related-genes-during-the-adipocyte-differentiation-using-public-access-data
#17
Mahmoud Ahmed, Huynh Quoc Nguyen, Jin Seok Hwang, Sahib Zada, Trang Huyen Lai, Sang Soo Kang, Deok Ryong Kim
Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we systematically describe the role of autophagy-related genes and gene sets during the differentiation of adipocytes. We used a public dataset from the European Nucleotide Archive from an RNA-seq experiment in which 3T3-L1 cells were induced by a differentiation induction medium, total RNA was extracted and sequenced at four different time points. Raw reads were aligned to the UCSC mouse reference genome (mm10) using HISAT2, and aligned reads were summarized at the gene or exon level using HTSeq...
March 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29643528/-comparison-of-the-properties-of-cd146-positive-and-cd146-negative-subpopulations-of-stem-cells-from-human-exfoliated-deciduous-teeth
#18
X T Wang, N Q Rao, T Jz Fang, Y M Zhao, L H Ge
OBJECTIVE: Stem cells from human exfoliated teeth (SHED) were sorted by magnetically activated cell sorting (MACS) technique to obtain the CD146 positive and negative cell subpopulation. Then the biological characteristics of these subpopulations were compared to explore their specific application potential in tissue engineering. METHODS: In this study, freshly extracted deciduous teeth without any caries or dental pulp disease were obtained. SHED was isolated using enzyme digestion method and then sorted by MACS, CD146 positive cells and CD146 negative cells were obtained after cell sorting...
April 18, 2018: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29628936/clinical-and-functional-characterization-of-a-missense-elf2-variant-in-a-canvas-family
#19
Hena Ahmad, Teresa Requena, Lidia Frejo, Marien Cobo, Alvaro Gallego-Martinez, Francisco Martin, Jose A Lopez-Escamez, Adolfo M Bronstein
Cerebellar ataxia with neuropathy and bilateral vestibular areflexia syndrome (CANVAS) is a rare disorder with an unknown etiology. We present a British family with presumed autosomal dominant CANVAS with incomplete penetrance and variable expressivity. Exome sequencing identified a rare missense variant in the ELF2 gene at chr4:g.140058846 C > T, c.10G > A, p.A4T which segregated in all affected patients. By using transduced BE (2)-M17 cells, we found that the mutated ELF2 (mt-ELF2) gene increased ATXN2 and reduced ELOVL5 gene expression, the causal genes of type 2 and type 38 spinocerebellar ataxias...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29627311/lysosomotropic-cationic-amphiphilic-drugs-inhibit-adipocyte-differentiation-in-3t3-l1k-cells-via-accumulation-in-cells-and-phospholipid-membranes-and-inhibition-of-autophagy
#20
Patrik Kagebeck, Violetta Nikiforova, Lars Brunken, Arrabi Easwaranathan, Joelle Ruegg, Ian Cotgreave, Vesna Munic Kos
Some cationic amphiphilic drugs (CADs) have been individually reported to interfere with the differentiation of immune system cells, such as macrophages and dendritic cells. To investigate the possible generic nature of this process, in this study we aimed to see whether these drugs are capable of interfering with the differentiation of adipocytes. Further, we investigated whether this feature might be connected to the lysosomotropic character of these drugs, and their disturbance of intracellular membrane trafficking rather than to the individual pharmacologic properties of each drug...
April 5, 2018: European Journal of Pharmacology
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