keyword
MENU ▼
Read by QxMD icon Read
search

Lipid droplets

keyword
https://www.readbyqxmd.com/read/28735096/lipid-droplet-functions-beyond-energy-storage
#1
REVIEW
Michael A Welte, Alex P Gould
Lipid droplets are cytoplasmic organelles that store neutral lipids and are critically important for energy metabolism. Their function in energy storage is firmly established and increasingly well characterized. However, emerging evidence indicates that lipid droplets also play important and diverse roles in the cellular handling of lipids and proteins that may not be directly related to energy homeostasis. Lipid handling roles of droplets include the storage of hydrophobic vitamin and signaling precursors, and the management of endoplasmic reticulum and oxidative stress...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28735095/regulation-of-lipid-droplet-associated-proteins-by-peroxisome-proliferator-activated-receptors
#2
REVIEW
Montserrat A de la Rosa Rodriguez, Sander Kersten
Excess fatty acids are stored in cells as triglycerides in specialized organelles called lipid droplets (LD). LD can be found in nearly all cell types and may expand during certain (patho)physiological conditions. The synthesis and breakdown of triglycerides and their deposition in LD is governed by a diverse set of enzymes and LD-associated proteins. These proteins serve structural roles in and around LD and regulate the activity of key lipogenic and lipolytic enzymes. The LD-associated proteins are subject to multiple regulatory mechanisms at the protein and gene expression level...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28734827/multi-omics-analyses-of-starvation-responses-reveal-a-central-role-for-lipoprotein-metabolism-in-acute-starvation-survival-in-c-%C3%A2-elegans
#3
Eva Bang Harvald, Richard R Sprenger, Kathrine Brændgaard Dall, Christer S Ejsing, Ronni Nielsen, Susanne Mandrup, Alejandro Brenes Murillo, Mark Larance, Anton Gartner, Angus I Lamond, Nils J Færgeman
Starvation causes comprehensive metabolic changes, which are still not fully understood. Here, we used quantitative proteomics and RNA sequencing to examine the temporal starvation responses in wild-type Caenorhabditis elegans and animals lacking the transcription factor HLH-30. Our findings show that starvation alters the abundance of hundreds of proteins and mRNAs in a temporal manner, many of which are involved in central metabolic pathways, including lipoprotein metabolism. We demonstrate that premature death of hlh-30 animals under starvation can be prevented by knockdown of either vit-1 or vit-5, encoding two different lipoproteins...
July 12, 2017: Cell Systems
https://www.readbyqxmd.com/read/28727846/effects-of-high-intensity-interval-versus-mild-intensity-endurance-training-on-metabolic-phenotype-and-corticosterone-response-in-rats-fed-a-high-fat-or-control-diet
#4
Youqing Shen, Guoyuan Huang, Bryan P McCormick, Tao Song, Xiangfeng Xu
The aim of the present study was to compare the effects of high-intensity interval training (HI) to mild-intensity endurance training (ME), combined with a high-fat diet (HFD) or control diet (CD) on metabolic phenotype and corticosterone levels in rats. Fifty-three rats were randomized to 6 groups according to diet and training regimen as follows: CD and sedentary (CS, n = 11), CD and ME (CME, n = 8), CD and HI (CHI, n = 8), HFD and sedentary (HS, n = 10), HFD and ME (HME, n = 8), and HFD and HI (HHI, n = 8)...
2017: PloS One
https://www.readbyqxmd.com/read/28727095/bta-mir-130a-regulates-the-biosynthesis-of-bovine-milk-fat-by-targeting-peroxisome-proliferator-activated-receptor-gamma
#5
W C Yang, W L Guo, L S Zan, Y N Wang, K Q Tang
Milk fat determines the quality of milk and is also a main targeted trait in dairy cow breeding. Recent studies have revealed important regulatory roles of microRNAs (miRNA) in milk fat synthesis in the mammary gland. However, the role of miRNA in bovine mammary epithelial cells (BMEC) remains largely unknown. In this study, we found that the overexpression of miR-130a significantly decreased cellular triacylglycerol (TAG) levels and suppressed lipid droplet formation, whereas the inhibition of miR-130a resulted in greater lipid droplet formation and TAG accumulation in BMEC...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726765/oleic-acid-uptake-reveals-the-rescued-enterocyte-phenotype-of-colon-cancer-caco-2-by-ht29-mtx-cells-in-co-culture-mode
#6
Emmanuelle Berger, Merian Nassra, Claude Atgié, Pascale Plaisancié, Alain Géloën
Gastrointestinal epithelium is the unique route for nutrients and for many pharmaceuticals to enter the body. The present study aimed to analyze precisely whether co-culture of two colon cancer cell lines, mucus-producing cells HT29-MTX and enterocyte-like Caco-2 cells, ameliorate differentiation into an in vitro intestinal barrier model and the signaling pathways involved. Differentiated Caco-2 cells gene datasets were compared first to intestinal or cancer phenotypes and second to signaling pathway gene datasets...
July 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28725267/heterologous-co-expression-of-a-yeast-diacylglycerol-acyltransferase-scdga1-and-a-plant-oleosin-atoleo3-as-an-efficient-tool-for-enhancing-triacylglycerol-accumulation-in-the-marine-diatom-phaeodactylum-tricornutum
#7
Nodumo Nokulunga Zulu, Jennifer Popko, Krzysztof Zienkiewicz, Pablo Tarazona, Cornelia Herrfurth, Ivo Feussner
BACKGROUND: Microalgae are promising alternate and renewable sources for producing valuable products such as biofuel and essential fatty acids. Although this is the case, there are still challenges impeding on the effective commercial production of microalgal products. For instance, their product yield is still too low. Therefore, this study was oriented towards enhancing triacylglycerol (TAG) accumulation in the diatom Phaeodactylum tricornutum (strain Pt4). To achieve this, a type 2 acyl-CoA:diacylglycerol acyltransferase from yeast (ScDGA1) and the lipid droplet (LD) stabilizing oleosin protein 3 from Arabidopsis thaliana (AtOLEO3) were expressed in Pt4...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28720784/snap-tagged-chikungunya-virus-replicons-improve-visualisation-of-non-structural-protein-3-by-fluorescence-microscopy
#8
Roland Remenyi, Grace C Roberts, Carsten Zothner, Andres Merits, Mark Harris
Chikungunya virus (CHIKV), a mosquito-borne alphavirus, causes febrile disease, muscle and joint pain, which can become chronic in some individuals. The non-structural protein 3 (nsP3) plays essential roles during infection, but a complete understanding of its function is lacking. Here we used a microscopy-based approach to image CHIKV nsP3 inside human cells. The SNAP system consists of a self-labelling enzyme tag, which catalyses the covalent linking of exogenously supplemented synthetic ligands. Genetic insertion of this tag resulted in viable replicons and specific labelling while preserving the effect of nsP3 on stress granule responses and co-localisation with GTPase Activating Protein (SH3 domain) Binding Proteins (G3BPs)...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720531/molecular-cloning-and-characterization-of-the-genes-encoding-the-proteins-of-zika-virus
#9
Wangheng Hou, Ruth Cruz-Cosme, Najealicka Armstrong, Lilian Akello Obwolo, Fayuan Wen, Wenhui Hu, Min-Hua Luo, Qiyi Tang
Zika virus (ZIKV) encodes a precursor protein (also called polyprotein) of about 3424 amino acids that is processed by proteases to generate 10 mature proteins and a small peptide. In the present study, we characterized the chemical features, suborganelle distribution and potential function of each protein using Flag-tagged protein expression system. Western blot analysis revealed the molecular weight of the proteins and the polymerization of E, NS1, and NS3 proteins. In addition, we performed multi-labeled fluorescent immunocytochemistry and subcellular fractionation to determine the subcellular localization of these proteins in host cells...
July 15, 2017: Gene
https://www.readbyqxmd.com/read/28719542/cideb-deficiency-aggravates-dextran-sulfate-sodium-induced-ulcerative-colitis-in-mice-by-exacerbating-the-oxidative-burden-in-colonic-mucosa
#10
Chao Sun, Yuanlin Zhao, Xing Gao, Yuan Yuan, Chao Wang, Yingmei Wang, Lijun Zhang, Yu Gu, Feng Zhang, Peizhen Hu, Zhe Wang, Zhuyi Li, Jiankuan Shi, Sheng Li, Kaiyu Cao, Bingcheng Ren, Ming Yu, Weihua Liang, Zengshan Li, Jing Ye
BACKGROUND: Abnormal lipid metabolism is one of many factors that contribute to the development of ulcerative colitis (UC). As a lipid droplet-associated protein, Cideb facilitated the export of lipids from enterocytes and promoted intestinal lipid absorption. We found that Cideb was upregulated in the colonic mucosa of both UC patients and dextran sodium sulfate (DSS)-induced mouse colitis, but its roles in the pathogenesis of UC are still ill-defined. METHODS: Acute colitis was induced with DSS in Cideb-null and wild-type mice, and the inflammation and oxidative stress were evaluated in the colonic mucosa...
August 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28719098/a-pyridoindole-based-multifunctional-bioprobe-ph-induced-fluorescence-switching-and-specific-targeting-of-lipid-droplets
#11
Bahadur Sk, Pilendra Thakre, Raghuvir Singh Tomar, Abhijit Patra
A versatile fluorescent probe, PITE, based on alkyl substituted pyridoindole (PI) and tetraphenylethylene (TE) exhibiting facile pH-induced fluorescence switching in solution, nanoparticles and solid state is presented. Strong fluorescence in the solid state besides solution and aggregated state allow sensing of toxic acid vapor. Fluorescence "off-on" switching of PITE by exposing to trifluoracetic acid and triethylamine vapor is visualized through naked eyes. A unified picture of switchable fluorescence of PITE is obtained by comprehensive spectroscopic investigations coupled with quantum mechanical calculations...
July 18, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28715671/adipose-triglyceride-lipase-protein-abundance-and-translocation-to-the-lipid-droplet-increase-during-leptin-induced-lipolysis-in-bovine-adipocytes
#12
D A Koltes, M E Spurlock, D M Spurlock
Proper regulation of lipid metabolism is critical for preventing the development of metabolic diseases. It is clear that leptin plays a critical role in the regulation of energy homeostasis by regulating energy intake. However, leptin can also regulate energy homeostasis by inducing lipolysis in adipocytes, but it is unclear how the major lipases are involved in leptin-stimulated lipolysis. Therefore, the objectives of this study were to determine if (1) leptin acts directly to induce lipolysis in bovine adipocytes, (2) the potential lipases involved in leptin-induced lipolysis in bovine adipocytes, and (3) increases translocation of adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) during leptin-stimulated lipolysis in bovine stromal vascular cell-derived adipocytes...
June 8, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28714972/organelle-biogenesis-in-the-endoplasmic-reticulum
#13
REVIEW
Amit S Joshi, Hong Zhang, William A Prinz
Understanding organelle biogenesis is a central focus of cell biology. Whereas some are generated from existing organelles, others can be generated de novo. Most de novo organelle biogenesis occurs in the endoplasmic reticulum (ER). Here, we review the role of the ER in the generation of peroxisomes, lipid droplets, and omegasomes, which are platforms for autophagosome production, and discuss how ER subdomains with specific protein and lipid composition form and promote organelle biogenesis.
July 17, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28713910/anti-obesity-effects-of-yellow-catfish-protein-hydrolysate-on-mice-fed-a-45-kcal-high-fat-diet
#14
Mi-Ryung Kim, Joo-Wan Kim, Jeong Been Park, Yong-Ki Hong, Sae Kwang Ku, Jae-Suk Choi
Obesity contributes to the etiologies of a variety of comorbid conditions, such as type 2 diabetes, hypertension and cardiovascular disease. In the present study, the anti-obesity effects of yellow catfish protein hydrolysate (YPh) were observed in mice fed a 45% kcal high-fat diet (HFD) compared with those of mice treated with simvastatin. The HFD-fed control mice exhibited noticeable increase in body weight, and whole-body and abdominal fat densities, periovarian and abdominal wall-deposited fat pad weight, as well as in the levels of triglycerides (TG), blood total cholesterol (TC), low-density lipoprotein, alanine aminotransferase, aspartate aminotransferase, creatinine, blood urea nitrogen, and in the fecal TG and TC contents...
July 10, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28712927/role-of-hepatic-annexin-a6-in-fatty-acid-induced-lipid-droplet-formation
#15
Rose Cairns, Anna Alvarez-Guaita, Inés Martínez-Saludes, Sundeep J Wason, Jacky Hanh, Shilpa R Nagarajan, Elham Hosseini-Beheshti, Katia Monastyrskaya, Andrew J Hoy, Christa Buechler, Carlos Enrich, Carles Rentero, Thomas Grewal
Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. More recently, AnxA6 has also been linked to triglyceride storage in adipocytes. Here we investigated the potential role of AnxA6 in fatty acid (FA) - induced lipid droplet (LD) formation in hepatocytes. AnxA6 was associated with LD from rat liver and HuH7 hepatocytes. In oleic acid (OA) -loaded HuH7 cells, substantial amounts of AnxA6 bound to LD in a Ca(2+)-independent manner...
July 13, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28711832/biodegradation-of-phenol-via-meta-cleavage-pathway-triggers-de-novo-tag-biosynthesis-pathway-in-oleaginous-yeast
#16
Alok Patel, Km Sartaj, Neha Arora, Vikas Pruthi, Parul A Pruthi
Phenol is reported to be one of the most toxic environmental pollutants present in the discharge of various industrial effluents causing a serious threat to the existing biome. Biodegradation of phenol by oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 was found to degrade 1000mg/l phenol. The pathways for phenol degradation by both ortho and meta-cleavage were proposed by the identification of metabolites and enzymatic assays of ring cleavage enzymes in the cell extracts. Results suggest that this oleaginous yeast degrade phenol via meta-cleavage pathway and accumulates a high quantity of lipid content (64...
July 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28711458/the-collaborative-work-of-droplet-assembly
#17
REVIEW
Xiao Chen, Joel M Goodman
Three proteins have been implicated in the assembly of cytoplasmic lipid droplets: seipin, FIT2, and perilipin. This review examines the current theories of seipin function as well as the evidence for the involvement of all three proteins in droplet biogenesis, and ends with a proposal of how they collaborate to regulate the formation of droplets. This article is part of a Special Issue entitled: Recent Advances in Lipid Droplet Biology edited by Rosalind Coleman and Matthijs Hesselink.
July 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28705617/drug-solubility-in-lipid-nanocarriers-influence-of-lipid-matrix-and-available-interfacial-area
#18
Katrin Göke, Heike Bunjes
Amongst other strategies for the formulation of poorly water-soluble drugs, solubilization of these drugs in lipid-based formulations is a promising option. Most screening methods for the identification of a suitable lipid-based formulation fail to elucidate the role interfacial effects play for drug solubility in disperse systems. In a novel screening approach called passive drug loading, different preformed lipid nanocarrier dispersions are incubated with drug powder. Afterwards, undissolved drug is filtered off and the amount of solubilized drug is determined...
July 10, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28705489/the-effects-of-estradiol-valerate-and-remifemin-on-liver-lipid-metabolism
#19
Biao Jin, Wenjuan Wang, Wenpei Bai, Jing Zhang, Ke Wang, Lihua Qin
To investigate the lipid metabolism dysregulation in the liver of ovariectomized (OVX) rats and effects of estradiol valerate (E) and remifemin (ICR) thereon, forty female Sprague-Dawley rats were randomly divided into sham-operated (SHAM), OVX, OVX+E, and OVX+ICR group. After 4 weeks' E or ICR treatment, serum estrogen, cholesterol, and triglyceride levels; lipid droplets in hepatocytes; hepatocyte morphology; and the expression of estrogen receptor α (ERα), liver X receptor (LXR), and sterol regulatory element binding proteins (SREBPs) in the liver of the rats were assessed...
July 11, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28703289/maresin-1-inhibits-tnf-alpha-induced-lipolysis-and-autophagy-in-3t3-l1-adipocytes
#20
Laura M Laiglesia, Silvia Lorente-Cebrián, Miguel López-Yoldi, Raquel Lanas, J Alfredo Martínez, Maria J Moreno-Aliaga
Obesity is associated with high pro-inflammatory cytokine levels like tumor necrosis factor-alpha (TNF-α), which promotes inflammation in adipose tissue. The omega-3 PUFAs, and their derived lipid mediators, such as Maresin 1 (MaR1) have anti-inflammatory effects on adipose tissue. This study aimed to analyze if MaR1 may counteract alterations induced by TNF-α on lipolysis and autophagy in mature 3T3-L1 adipocytes. Our data revealed that MaR1 (1-100 nM) inhibited the TNF-α-induced glycerol release after 48 h, which may be related to MaR1 ability of preventing the decrease of lipid droplet-coating protein perilipin and G0/G1 Switch 2 protein expression...
July 13, 2017: Journal of Cellular Physiology
keyword
keyword
53776
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"