Aldana Trabucchi, Silvina Sonia Bombicino, Juan Ignacio Marfía, Adriana Victoria Sabljic, Rubén Francisco Iacono, Ignacio Smith, Gregorio Juan Mc Callum, Alexandra Marisa Targovnik, Federico Javier Wolman, Matías Fingermann, Leonardo Gabriel Alonso, María Victoria Miranda, Silvina Noemí Valdez
Considering the course of the current SARS-CoV-2 pandemic, it is important to have serological tests for monitoring humoral immune response against SARS-CoV-2 infection and vaccination. Herein we describe a novel bridge enzyme-linked immunosorbent assay (b-ELISA) for SARS-CoV-2 antibodies detection in human and other species, employing recombinant Spike protein as a unique antigen, which is produced at high scale in insect larvae. METHODS: Eighty two human control sera/plasmas and 169 COVID-19 patients' sera/plasmas, confirmed by rRT-PCR, were analyzed by the b-ELISA assay...
October 3, 2022: Journal of Immunological Methods