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https://www.readbyqxmd.com/read/27922827/gold-nanoparticles-as-scaffolds-for-poor-water-soluble-and-difficult-to-vehiculate-antiparkinson-codrugs
#1
A Di Crescenzo, I Cacciatore, M Petrini, M D'Alessandro, N Petragnani, P Del Boccio, P Di Profio, S Boncompagni, G Spoto, H Turkez, P Ballerini, A Di Stefano, A Fontana
We report the facile and non-covalent preparation of gold nanoparticles (AuNPs) stabilized by an antiparkinson codrug based on lipoic acid (LA). The obtained AuNPs appear stable in both dimethyl sulfoxide and fetal bovine serum and able to load an amount of codrug double the weight of gold. These NPs were demonstrated to be safe and biocompatible towards primary human blood cells and human neuroblastoma cells, one of the most widely used cellular models to study dopaminergic neural cells, therefore are ideal drug carriers for difficult to solubilize molecules...
December 6, 2016: Nanotechnology
https://www.readbyqxmd.com/read/27922688/berberine-exerts-an-anti-inflammatory-role-in-ocular-behcet-s-disease
#2
Yan Yang, Qin Wang, Manyun Xie, Ping Liu, Xin Qi, Xiao Liu, Zhuo Li
Behcet's disease is a multi‑system inflammatory disorder, and ocular Behcet's disease (OBD) is one of the most common causes of uveitis in China. A number of studies have indicated that Th17 cells, a subset of interleukin-17 (IL-17)-producing CD4+ T‑helper cells, serve important roles in the pathogenesis of OBD. Berberine (BBR) is an isoquinoline derivative alkaloid isolated from Chinese herbs, and has been used traditionally for the treatment of gastrointestinal disorders. The aim of the present study was to investigate the effect of BBR on Th17 cell proliferation and cytokine secretion, and the expression and activation of the signal transducer and activator of transcription 3 (STAT3) transcription factor in OBD in vitro...
December 5, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27921389/superiority-of-sds-lysis-over-saponin-lysis-for-direct-bacterial-identification-from-positive-blood-culture-bottle-by-maldi-tof-ms
#3
Yvan Caspar, Cécile Garnaud, Mariya Raykova, Sébastien Bailly, Marie Bidart, Danièle Maubon
PURPOSE: Fast species diagnosis has an important health care impact, as rapid and specific antibacterial therapy is of clear benefit for patient's outcome. Here we propose a new protocol for species identification directly from positive blood cultures. EXPERIMENTAL DESIGN: We compared four in-house protocols for bacterial identification by mass spectrometry directly from clinical positive blood cultures evaluating 2 lytic agents, SDS and saponin, and 2 protein extraction schemes, fast (FP) and long (LP)...
December 6, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27920984/staphylococcus-lugdunensis-endocarditis-with-destruction-of-the-ventricular-septum-and-multiple-native-valves
#4
Celestine Ishiekwene, Monica Ghitan, Margaret Kuhn-Basti, Edward Chapnick, Yu Shia Lin
Staphylococcus lugdunensis (S. lugdunensis) is a coagulase negative staphylococcus (CoNS) that can cause destructive infective endocarditis. S. lugdunensis, unlike other CoNS, should be considered to be a pathogen. We report the first case of S. lugdunensis endocarditis causing ventricular septal defect and destruction of the aortic and mitral valves. A 53-year-old male with morbid obesity and COPD presented with intermittent fever and progressive shortness of breath for 2 weeks. Chest examination showed bilateral basal crepitations, and a grade 2 systolic murmur along the right sternal border...
2017: IDCases
https://www.readbyqxmd.com/read/27920983/bacteremic-renal-stone-associated-urinary-tract-infection-caused-by-nontypable-haemophilus-influenzae-a-rare-invasive-disease-in-an-immunocompetent-patient
#5
Masanao Fujii, Harumi Gomi, Haruhiko Ishioka, Noriko Takamura
Haemophilus species are known to colonize the upper respiratory tract and can cause infections. However Haemophilus influenzae has been rarely described as a cause of genitourinary tract infection. We report a 44-year-old nonimmunocompromised Japanese man with bacteremic pyelonephritis caused by a nontypable H. influenzae associated with a left ureteral calculus. The organism was isolated from both blood and urine cultures. Treatment consisted of 14 days of intravenous ceftriaxone and oral amoxicillin one after than other and insertion of a left ureteral stent...
2017: IDCases
https://www.readbyqxmd.com/read/27920981/infective-endocarditis-and-sj%C3%A3-gren-s-syndrome-diagnosed-simultaneously
#6
Fujiko Morita, Yuji Hirai, Kiyozumi Suzuki, Yuki Uehara, Kazunori Mitsuhashi, Atsushi Amano, Toshio Naito
Poor dentition and/or dental infection due to insufficient oral care are presumed to be risk factors for infective endocarditis (IE). We present a case of endocarditis caused by Granulicatella adiacens and Sjögren's syndrome (SS) with oral complications diagnosed simultaneously. A 67-year-old woman was admitted to our hospital with fever, general fatigue, arthralgia, and back pain. She was diagnosed with primary SS according to the criteria of the American-European Consensus Group. Transthoracic echocardiography carried out to examine her persistent fever revealed vegetation formation (14 × 5 mm) on the aortic valve and her blood cultures were positive for G...
2017: IDCases
https://www.readbyqxmd.com/read/27920090/islet-derived-cd4-t-cells-targeting-proinsulin-in-human-autoimmune-diabetes
#7
Aaron W Michels, Laurie G Landry, Kristen A McDaniel, Liping Yu, Martha Campbell-Thompson, William W Kwok, Kenneth L Jones, Peter A Gottlieb, John W Kappler, Qizhi Tang, Bart O Roep, Mark A Atkinson, Clayton E Mathews, Maki Nakayama
Type 1 diabetes results from chronic autoimmune destruction of insulin-producing beta cells within pancreatic islets. While insulin is a critical self-antigen in animal models of autoimmune diabetes, due to extremely limited access to pancreas samples, little is known about human antigenic targets for islet-infiltrating T-cells. Here we show that proinsulin peptides are targeted by islet-infiltrating T-cells from type 1 diabetes patients. We identified hundreds of T-cells from inflamed pancreatic islets of three young type 1 diabetes organ donors with a short disease duration with high risk HLA genes using a direct T-cell receptor (TCR) sequencing approach without long-term cell culture...
December 5, 2016: Diabetes
https://www.readbyqxmd.com/read/27920058/a-novel-somatic-mutation-achieves-partial-rescue-in-a-child-with-hutchinson-gilford-progeria-syndrome
#8
Daniel Z Bar, Martin F Arlt, Joan F Brazier, Wendy E Norris, Susan E Campbell, Peter Chines, Delphine Larrieu, Stephen P Jackson, Francis S Collins, Thomas W Glover, Leslie B Gordon
BACKGROUND: Hutchinson-Gilford progeria syndrome (HGPS) is a fatal sporadic autosomal dominant premature ageing disease caused by single base mutations that optimise a cryptic splice site within exon 11 of the LMNA gene. The resultant disease-causing protein, progerin, acts as a dominant negative. Disease severity relies partly on progerin levels. METHODS AND RESULTS: We report a novel form of somatic mosaicism, where a child possessed two cell populations with different HGPS disease-producing mutations of the same nucleotide-one producing severe HGPS and one mild HGPS...
December 5, 2016: Journal of Medical Genetics
https://www.readbyqxmd.com/read/27919308/outbreak-of-pantoea-agglomerans-bloodstream-infections-at-an-oncology-clinic-illinois-2012-2013
#9
Brian R Yablon, Raymund Dantes, Victoria Tsai, Rachel Lim, Heather Moulton-Meissner, Matthew Arduino, Bette Jensen, Megan Toth Patel, Michael O Vernon, Yoran Grant-Greene, Demian Christiansen, Craig Conover, Alexander Kallen, Alice Y Guh
OBJECTIVE To determine the source of a healthcare-associated outbreak of Pantoea agglomerans bloodstream infections. DESIGN Epidemiologic investigation of the outbreak. SETTING Oncology clinic (clinic A). METHODS Cases were defined as Pantoea isolation from blood or catheter tip cultures of clinic A patients during July 2012-May 2013. Clinic A medical charts and laboratory records were reviewed; infection prevention practices and the facility's water system were evaluated. Environmental samples were collected for culture...
December 6, 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27919230/the-association-of-hyperglycaemia-with-prevalent-tuberculosis-a-population-based-cross-sectional-study
#10
Sarah Lou Bailey, Helen Ayles, Nulda Beyers, Peter Godfrey-Faussett, Monde Muyoyeta, Elizabeth du Toit, John S Yudkin, Sian Floyd
BACKGROUND: Systematic reviews suggest that the incidence of diagnosed tuberculosis is two- to- three times higher in those with diabetes mellitus than in those without. Few studies have previously reported the association between diabetes or hyperglycaemia and the prevalence of active tuberculosis and none in a population-based study with microbiologically-defined tuberculosis. Most have instead concentrated on cases of diagnosed tuberculosis that present to health facilities. We had the opportunity to measure glycaemia alongside prevalent tuberculosis...
December 5, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27918869/paramedic-initiated-cms-sepsis-core-measure-bundle-prior-to-hospital-arrival-a-stepwise-approach
#11
Jason G Walchok, Ronald G Pirrallo, Douglas Furmanek, Martin Lutz, Colt Shope, Brandi Giles, Greta Gue, Aaron Dix
BACKGROUND: To improve patient outcomes, the Center for Medicare and Medicaid Services (CMS) implemented core measures that outline the initial treatment of the septic patient. These measures include initial blood culture collection prior to antibiotics, adequate intravenous fluid resuscitation, and early administration of broad spectrum antibiotics. We sought to determine if Paramedics can initiate the CMS sepsis core measure bundle in the prehospital field reliably. METHODS: This is a retrospective, case series from a 3rd service EMS system model in Greenville, South Carolina between November 17, 2014 and February 20, 2016...
December 5, 2016: Prehospital Emergency Care
https://www.readbyqxmd.com/read/27918730/il-4-induces-the-formation-of-multinucleated-giant-cells-and-expression-of-%C3%AE-5-integrin-in-central-giant-cell-lesion
#12
A Aghbali, S Rafieyan, L Mohamed-Khosroshahi, B Baradaran, D Shanehbandi, M Kouhsoltani
BACKGROUND: It is now well established that IL-4 has a central role in the development of monocytes to multinucleated giant cells (MGCs) by inducing the expression of integrins on the surface of monocytes. The aim of this study was to investigate the potential role of IL-4 in induction of β5 integrin expression in the peripheral blood samples of patients with giant cell granuloma. MATERIAL AND METHODS: Monocytes were isolated from peripheral blood samples of patients with central giant cell granuloma (CGCG) and healthy controls using human Monocyte Isolation Kit II...
December 6, 2016: Medicina Oral, Patología Oral y Cirugía Bucal
https://www.readbyqxmd.com/read/27918471/polyphenols-and-dna-damage-a-mixed-blessing
#13
REVIEW
Amaya Azqueta, Andrew Collins
Polyphenols are a very broad group of chemicals, widely distributed in plant foods, and endowed with antioxidant activity by virtue of their numerous phenol groups. They are widely studied as putative cancer-protective agents, potentially contributing to the cancer preventive properties of fruits and vegetables. We review recent publications relating to human trials, animal experiments and cell culture, grouping them according to whether polyphenols are investigated in whole foods and drinks, in plant extracts, or as individual compounds...
December 3, 2016: Nutrients
https://www.readbyqxmd.com/read/27918018/a-planar-model-of-the-vessel-wall-from-cellularized-collagen-scaffolds-focus-on-cell-matrix-interactions-in-mono-bi-and-tri-culture-models
#14
Caroline Loy, Sébastien Meghezi, Lucie Lévesque, Daniele Pezzoli, Heena Kumra, Dieter Reinhardt, Jayachandran N Kizhakkedathu, Diego Mantovani
The acquisition of new thorough knowledge on the interactions existing between vascular cells would represent a step forward in the engineering of vascular tissues. In this light, herein we designed a physiological-like tri-culture in vitro vascular wall model using a planar cellularized collagen gel as the scaffold. The model can be obtained in 24 h and features multi-layered hierarchical organization composed of a fibroblast-containing adventitia-like layer, a media-like layer populated by smooth muscle cells and an intima-like endothelial cell monolayer...
December 5, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27917737/a-pediatric-approach-to-ventilator-associated-events-surveillance
#15
Noelle M Cocoros, Gregory P Priebe, Latania K Logan, Susan Coffin, Gitte Larsen, Philip Toltzis, Thomas J Sandora, Marvin Harper, Julia S Sammons, James E Gray, Donald Goldmann, Kelly Horan, Michael Burton, Paul A Checchia, Matthew Lakoma, Shannon Sims, Michael Klompas, Grace M Lee
OBJECTIVE Adult ventilator-associated event (VAE) definitions include ventilator-associated conditions (VAC) and subcategories for infection-related ventilator-associated complications (IVAC) and possible ventilator-associated pneumonia (PVAP). We explored these definitions for children. DESIGN Retrospective cohort SETTING Pediatric, cardiac, or neonatal intensive care units (ICUs) in 6 US hospitals PATIENTS Patients ≤18 years old ventilated for ≥1 day METHODS We identified patients with pediatric VAC based on previously proposed criteria...
December 5, 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27917316/quantitative-analysis-of-rat-adipose-tissue-cell-recovery-and-non-fat-cell-volume-in-primary-cell-cultures
#16
Floriana Rotondo, María Del Mar Romero, Ana Cecilia Ho-Palma, Xavier Remesar, José Antonio Fernández-López, Marià Alemany
BACKGROUND: White adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defense, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT by breaking up the matrix of collagen fibres; however, it is unclear to what extent adipocyte number in primary cultures correlates with their number in intact WAT, since recovery and viability are often unknown...
2016: PeerJ
https://www.readbyqxmd.com/read/27917206/anticancer-and-antibacterial-secondary-metabolites-from-the-endophytic-fungus-penicillium-sp-cam64-against-multi-drug-resistant-gram-negative-bacteria
#17
Jean-Bosco Jouda, Jean-de-Dieu Tamokou, Céline Djama Mbazoa, Prodipta Sarkar, Prasanta Kumar Bag, Jean Wandji
BACKGROUND: The emergence of multiple-drug resistance bacteria has become a major threat and thus calls for an urgent need to search for new effective and safe anti-bacterial agents. OBJECTIVES: This study aims to evaluate the anticancer and antibacterial activities of secondary metabolites from Penicillium sp., an endophytic fungus associated with leaves of Garcinia nobilis. METHODS: The culture filtrate from the fermentation of Penicillium sp...
September 2016: African Health Sciences
https://www.readbyqxmd.com/read/27917016/the-portapotty-experiment-neoliberal-approaches-to-the-intertwined-epidemics-of-opioid-related-overdose-and-hiv-hcv-and-why-we-need-cultural-anthropologists-in-the-south-bronx
#18
Brett Wolfson-Stofko, Ric Curtis, Faustino Fuentes, Ed Manchess, Alexis Del Rio-Cumba, Alex S Bennett
The following report from the field focuses on the authors' collective efforts to operate an ad hoc safer injection facility (SIF) out of portapotties (portable toilets) in an area of the South Bronx that has consistently experienced some of the highest overdose morbidity and mortality rates in New York City over the past decade (New York City Department of Health and Mental Hygiene, 2011, 2015, 2016). Safer injection facilities (also known as supervised injection facilities, drug consumption rooms, etc.) operating outside the US provide a legal, hygienic, and supervised environment for individuals to use drugs in order to minimize the likelihood of fatal overdose and the spread of blood-borne infections while reducing public injection...
December 2016: Dialectical Anthropology
https://www.readbyqxmd.com/read/27916472/generation-of-neural-cells-using-ipscs-from-sleep-bruxism-patients-with-5-ht2a-polymorphism
#19
Yurie Hoashi, Satoshi Okamoto, Yuka Abe, Takashi Matsumoto, Junichi Tanaka, Yuya Yoshida, Kent Imaizumi, Kenji Mishima, Wado Akamatsu, Hideyuki Okano, Kazuyoshi Baba
PURPOSE: Sleep bruxism (SB) is classified as a sleep-related movement disorder characterized by grinding and clenching of the teeth during sleep, which is responsible for a variety of clinical problems such as abnormal tooth attrition and fracture of teeth or roots. Little is known about the etiology of SB. Our previous study identified a genomic association of the serotonin 2A receptor (5-HT2A) single nucleotide polymorphism (SNP), rs6313 C>T, with SB, where the C allele carrier is associated with a 4...
December 1, 2016: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/27916353/toll-like-receptor-responses-are-suppressed-in-trauma-icu-patients
#20
Travis L Holloway, Susannah E Nicholson, Meenakshi Rani, Andrew P Cap, Martin G Schwacha
BACKGROUND: Inflammation and activation of the innate immune system are often associated with traumatic injury and may involve alterations in toll-like receptor (TLR)-mediated responses. METHODS: A prospective observational study was designed and conducted. Twenty-one severely injured (ISS = 16-41) trauma intensive care unit (ICU) patients and six healthy volunteers that served as controls were enrolled. Anticoagulated whole blood was collected at 2-12 d after ICU admission and incubated in the presence of media alone (baseline), zymosan (TLR2 agonist) or lipopolysaccharide (LPS; TLR4 agonist) for 3 h...
November 2016: Journal of Surgical Research
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