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https://www.readbyqxmd.com/read/29044197/development-of-a-multiplex-probe-combination-based-one-step-real-time-reverse-transcription-pcr-for-na-subtype-typing-of-avian-influenza-virus
#1
Zhihao Sun, Tao Qin, Feifei Meng, Sujuan Chen, Daxin Peng, Xiufan Liu
Nine influenza virus neuraminidase (NA) subtypes have been identified in poultry and wild birds. Few methods are available for rapid and simple NA subtyping. Here we developed a multiplex probe combination-based one-step real-time reverse transcriptase PCR (rRT-PCR) to detect nine avian influenza virus NA subtypes. Nine primer-probe pairs were assigned to three groups based on the different fluorescent dyes of the probes (FAM, HEX, or Texas Red). Each probe detected only one NA subtype, without cross reactivity...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29044041/viral-haemorrhagic-septicaemia-virus-vhsv-id-infections-are-detected-more-consistently-using-syndromic-vs-active-surveillance
#2
Pia Vennerström, Elina Välimäki, Tapani Lyytikäinen, Maria Hautaniemi, Gabriele Vidgren, Perttu Koski, Anna-Maija Virtala
The eradication of viral haemorrhagic septicaemia virus (VHSV Id) from Finnish brackish-water rainbow trout Oncorhynchus mykiss farms located in the restriction zone in the Province of Åland, Baltic Sea, failed several times in the 2000s. The official surveillance programme was often unable to find VHSV-positive populations, leading to the misbelief in the fish farming industry that virus eradication could be achieved. The ability of 3 other surveillance programmes to detect infected fish populations was compared with the official programme...
October 18, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/29043869/identifying-the-biomarker-potential-of-telomerase-activity-and-shelterin-complex-molecule-telomeric-repeat-binding-factor-2-terf2-in-multiple-myeloma
#3
Raman Kumar, Rehan Khan, Nidhi Gupta, Tulika Seth, Atul Sharma, Mani Kalaivani, Alpana Sharma
Telomere length (TL) is maintained by telomere capping protein complex called shelterin complex. We studied the possible involvement and biomarker potential of shelterin complex molecules in naive multiple myeloma (MM) patients and controls. TL, relative telomerase activity (RTA), real-time PCR and Western blotting were performed in bonemarrow sample of 70 study subjects (patients = 50; controls = 20). Significantly lowered mean TL, increased RTA and higher mRNA expression of shelterin molecules were observed in patients, while PIN2/TERF1 interacting telomerase inhibitor 1 (PINX1) showed lower mRNA expression...
October 18, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/29043706/the-effects-of-exercise-on-expression-of-cyp19-and-star-mrna-in-steroid-induced-polycystic-ovaries-of-female-rats
#4
Fatemeh Aghaie, Homayoun Khazali, Mehdi Hedayati, Ali Akbarnejad
Background: Polycystic ovarian syndrome (PCOS) is the most frequent female endocrine disorder that affects 5-10% of women. PCOS is characterized by hyperandrogenism, oligo-/anovulation, and polycystic ovaries. The aim of the present research is to evaluate the expression of steroidogenic acute regulatory protein (StAR) and aromatase (CYP19) mRNA in the ovaries of an estradiol valerate (EV)-induced PCOS rat model, and the effect of treadmill and running wheel (voluntary) exercise on these parameters...
January 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29043533/corneal-cross-linking-has-no-effect-on-matrix-metalloproteinase-9-and-13-levels-during-fungal-keratitis-on-the-early-stage
#5
Ayse Kalkanci, Kamil Bilgihan, Huseyin Baran Ozdemir, Atiye Seda Yar Saglam, Funda Karakurt, Merve Erdogan
The aim of our study was to investigate matrix metalloproteinases, MMP-9 and MMP-13 levels, in the rabbit model of Fusarium and Candida keratitis treated by corneal cross-linking (PACK-CXL). Rabbit corneas were inoculated with fungal inoculum for keratitis. Each group divided into four subgroups, including un-treated group, PACK-CXL group, voriconazole group and PACK-CXL plus voriconazole group. PACK-CXL was applied with 0.25% riboflavin in accelerated Dresden protocol, and 0.1% voriconazole drops were administered...
October 17, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29042935/decreased-cathepsin-k-levels-in-human-atherosclerotic-plaques-are-associated-with-plaque-instability
#6
Huiying Zhao, Xiujiao Qin, Shuai Wang, Xiwei Sun, Bin Dong
Investigating the determinants and dynamics of atherosclerotic plaque instability is a key area of current cardiovascular research. Extracellular matrix degradation from excessive proteolysis induced by enzymes such as cathepsin K (Cat K) is implicated in the pathogenesis of unstable plaques. The current study assessed the expression of Cat K in human unstable atherosclerotic plaques. Specimens of popliteal arteries with atherosclerotic plaques were classified as stable (<40% lipid core plaque area; n=6) or unstable (≥40% lipid core plaque area; n=14) based on histopathological examinations of hematoxylin and eosin stained sections...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29039614/substitutions-of-rtl228-and-or-l229-are-involved-in-the-regulation-of-replication-and-hbsag-secretion-in-hepatitis-b-virus-and-do-not-affect-susceptibility-to-nucleos-t-ide-analogs
#7
Bo Qin, Yechao Zhou, Guozhong Zhou, Xiuping Xu, Yanan Wang, Jinkun Chen
Nucleos(t)ide analogs (NAs) are widely used in the treatment of hepatitis B virus (HBV) and human immunodeficiency virus (HIV). The mutation L210W of HIV‑1 reverse transcriptase (RT) is one of the six principal mutations which confer in vivo resistance to zidovudine. Due to the similar 3D‑structure and high conservation between HIV‑RT and HBV‑RT, the present study aimed to clarify whether corresponding mutations in HBV may decrease its susceptibility to relevant NAs. Mutations including rtL228C/W, rtL229W and rtL228W/L229W were introduced into a HBV replication competent plasmid by fusion polymerase chain reaction...
October 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29032022/outcomes-of-newly-diagnosed-chronic-phase-chronic-myeloid-leukemia-following-an-elective-switch-from-second-generation-tyrosine-kinase-inhibitor-to-imatinib
#8
Vamsi K Kota, Jee Hyun Kong, Martha Arellano, Fuad El Rassi, Manila Gaddh, Leonard T Heffner, Elliott F Winton, Anand P Jillella, Morgan L McLemore, H Jean Khoury
The second-generation tyrosine kinase inhibitors (TKIs) (2G-TKIs) dasatinib (DAS) and nilotinib (NIL) yield faster responses in newly diagnosed chronic phase (CP) chronic myeloid leukemia (CML) as compared with imatinib (IM); however, long-term safety of these agents is a growing concern. We identified 20 patients with CP-CML diagnosed between August 2013 and October 2016 who initiated 2G-TKIs and were then switched after optimal response at 3 months to IM. Second-generation TKIs initiated were DAS (n = 15), NIL (n = 3), or both sequentially due to intolerance (n = 1)...
September 19, 2017: Clinical Lymphoma, Myeloma & Leukemia
https://www.readbyqxmd.com/read/29030976/synergistic-effect-of-hif-1%C3%AE-and-foxo3a-trigger-cardiomyocyte-apoptosis-under-hyperglycemic-ischemia-condition
#9
Ya-Fang Chen, Sudhir Pandey, Cecilia Hsuan Day, Yu-Feng Chen, Ai-Zhi Jiang, Tsung-Jung Ho, Ray-Jade Chen, Vijaya PadmaViswanadha, Wei-Wen Kuo, Chih-Yang Huang
Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death...
October 14, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29029064/2015-2016-vaccine-effectiveness-of-live-attenuated-and-inactivated-influenza-vaccines-in-children-in-the-united-states
#10
Katherine A Poehling, Herve Caspard, Timothy R Peters, Edward A Belongia, Blaise Congeni, Manjusha Gaglani, Marie R Griffin, Stephanie A Irving, Poornima K Kavathekar, Huong Q McLean, Allison L Naleway, Kathleen Ryan, H Keipp Talbot, Christopher S Ambrose
Background: In the 2015-2016 season, quadrivalent live attenuated influenza vaccine (LAIV) and both trivalent and quadrivalent inactivated influenza vaccine (IIV) were available in the United States. Methods: This study, conducted according to a test-negative case-control design, enrolled children aged 2-17 years presenting to outpatient settings with fever and respiratory symptoms for <5 days at 8 sites across the United States between November 30, 2015 and April 15, 2016...
October 4, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29026462/murine-hepatocellular-carcinoma-derived-stem-cells-reveal-epithelial-to-mesenchymal-plasticity
#11
Aparna Jayachandran, Ritu Shrestha, Bijay Dhungel, I-Tao Huang, Marianna Yumi Kawashima Vasconcelos, Brian J Morrison, Charmaine A Ramlogan-Steel, Jason C Steel
AIM: To establish a model to enrich and characterize stem-like cells from murine normal liver and hepatocellular carcinoma (HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition (EMT). METHODS: In this study, we utilized a stem cell conditioned serum-free medium to enrich stem-like cells from mouse HCC and normal liver cell lines, Hepa 1-6 and AML12, respectively. We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating the RNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR (qRT-PCR)...
September 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/29022109/interaction-of-cd80-with-neph1-a-potential-mechanism-of-podocyte-injury
#12
Bhavya Khullar, Renu Balyan, Neelam Oswal, Nidhi Jain, Amita Sharma, Malik Z Abdin, Arvind Bagga, Shinjini Bhatnagar, Nitya Wadhwa, Uma Chandra Mouli Natchu, Anna George, Satyajit Rath, Vineeta Bal, Shailaja Sopory
BACKGROUND: The induction of CD80 on podocytes has been shown in animal models of podocyte injury and in certain cases of nephrotic syndrome. In a lipopolysaccharide (LPS)-induced mouse model of albuminuria, we have recently shown a signalling axis of LPS-myeloid cell activation-TNFα production-podocyte CD80 induction-albuminuria. Therefore, in this report, we investigated the cellular and molecular consequences of TNFα addition and CD80 expression on cultured podocytes. METHODS: A murine podocyte cell line was used for TNFα treatment and for over-expressing CD80...
October 11, 2017: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/29019910/determination-of-salmonella-spp-e-coli-vtec-vibrio-spp-and-norovirus-gi-gii-in-bivalve-molluscs-collected-from-growing-natural-beds-in-sardinia-italy
#13
Marta Marceddu, Sonia Lamon, Simonetta G Consolati, Sara Ciulli, Roberta Mazza, Anna Mureddu, Domenico Meloni
The aim of the present study was to evaluate the presence of Salmonella spp., verotoxigenic E. coli (VTEC), Vibrio spp., and Norovirus GI-GII in bivalve molluscs, cockles, and European grooved carpet shells (Cerastoderma spp. and Ruditapes decussatus) collected from a class B growing natural bed in Sardinia (Italy). All of the samples were analysed for Salmonella spp. detection according to European Commission Regulation (EC) 2285/2015. Detection and enumeration of Vibrio spp. were performed according to previously published methods...
October 11, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29018420/molecular-characterization-of-reduced-susceptibility-to-biocides-in-clinical-isolates-of-acinetobacter-baumannii
#14
Fei Lin, Ying Xu, Yaowen Chang, Chao Liu, Xu Jia, Baodong Ling
Active efflux is regarded as a common mechanism for antibiotic and biocide resistance. However, the role of many drug efflux pumps in biocide resistance in Acinetobacter baumannii remains unknown. Using biocide-resistant A. baumannii clinical isolates, we investigated the incidence of 11 known/putative antimicrobial resistance efflux pump genes (adeB, adeG, adeJ, adeT1, adeT2, amvA, abeD, abeM, qacE, qacEΔ1, and aceI) and triclosan target gene fabI through PCR and DNA sequencing. Reverse transcriptase quantitative PCR was conducted to assess the correlation between the efflux pump gene expression and the reduced susceptibility to triclosan or chlorhexidine...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29017417/aberrant-expression-of-lncrna-hoxa11-as1-and-homeobox-a-hoxa9-hoxa10-hoxa11-and-hoxa13-genes-in-infertile-women-with-endometriosis
#15
Meimei Wang, Cuifang Hao, Xin Huang, Hongchu Bao, Qinglan Qu, Zhenteng Liu, Huangguan Dai, Shunzhi He, Wei Yan
This study aimed to study the expression of homeobox (HOX)A11-AS1 ( HOXA11 antisense RNA) long noncoding RNA (lncRNA) and the expression of homeobox A ( HOXA9, HOXA10, HOXA11, and HOXA13) genes in the eutopic (EU) and ectopic (EC) endometria of women with peritoneal endometriosis. A total of 30 women undergoing laparoscopic surgery for peritoneal endometriosis and 15 infertile women without endometriosis were enrolled in this study. Peritoneal EC tissue samples were obtained through surgery. The EU tissues were obtained by curettage...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/29017189/curcumin-affects-adipose-tissue-derived-mesenchymal-stem-cell-aging-through-tert-gene-expression
#16
Samaneh Pirmoradi, Ezzatollah Fathi, Raheleh Farahzadi, Younes Pilehvar-Soltanahmadi, Nosratollah Zarghami
Aging and losing cell survival is one of the main problems in cell therapy. Aging of Mesenchymal Stem Cells (MSCs) is associated with a rise in intracellular reactive oxygen species, decrease in telomerase reverse transcriptase (TERT) expression and finally eroded telomere ends. Given that the production of free radicals in the aging process is effective, the use of antioxidants can help in scavenging free radicals and prevent the aging of cells. The aim of this study is to evaluate the effects of curcumin on proliferation, aging and TERT expression of rat adipose tissue-derived stem cells (rADSC)...
October 10, 2017: Drug Research
https://www.readbyqxmd.com/read/29016312/dengue-virus-serotype-2-established-in-northern-mozambique-2015-2016
#17
John Oludele, Birgitta Lesko, Isabel Mahumane Gundane, Fernanda de Bruycker-Nogueira, Argentina Muianga, Sadia Ali, Flora Mula, Imelda Chelene, Kerstin I Falk, Flávia Barreto Dos Santos, Eduardo Samo Gudo
After the report of an outbreak of dengue virus serotype 2 in 2014 in Nampula and Pemba cities, northern Mozambique, a surveillance system was established by the National Institute of Health. A study was performed during 2015-2016 to monitor the trend of the outbreak and confirm the circulating serotype of dengue virus (DENV). After the inclusion of consenting patients who met the case definition, samples from 192 patients were tested for the presence of nonstructural protein 1 antigen, and 60/192 (31%) samples were positive...
September 18, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28993274/in-situ-hybridization-detection-methods-for-hpv16-e6-e7-mrna-in-identifying-transcriptionally-active-hpv-infection-of-oropharyngeal-carcinoma-an-updating
#18
Chiara C Volpi, Chiara M Ciniselli, Ambra V Gualeni, Maddalena Plebani, Salvatore Alfieri, Paolo Verderio, Laura Locati, Federica Perrone, Pasquale Quattrone, Antonino Carbone, Silvana Pilotti, Annunziata Gloghini
The aim of this study is comparing two in situ hybridization (ISH) detection methods for human papilloma virus (HPV) 16 E6/E7 mRNA, i.e. the RNAscope® 2.0 High Definition (HD) and the upgraded RNAscope® 2.5 HD version. The RNAscope® 2.5 HD has recently replaced the RNAscope® 2.0 HD detection kit. Therefore, this investigation starts from the need to analytically validate the new mRNA ISH assay and, possibly, to refine the current algorithm for HPV detection in oropharyngeal squamous cell carcinoma (OSCC) with the final goal to apply it to daily laboratory practice...
October 6, 2017: Human Pathology
https://www.readbyqxmd.com/read/28990372/atp-binding-cassette-sub-family-member-2-abcg2-and-xenobiotic-exposure-during-early-mouse-embryonic-stem-cell-differentiation
#19
Mitchell B Rosen, Susan C Jeffay, Harriette P Nichols, Maria R Hoopes, E Sidney Hunter
BACKGROUND: ATP binding cassette sub-family member 2 (ABCG2) is a well-defined efflux transporter found in a variety of tissues. The role of ABCG2 during early embryonic development, however, is not established. Previous work which compared data from the ToxCast screening program with that from in-house studies suggested an association exists between exposure to xenobiotics that regulate Abcg2 transcription and differentiation of mouse embryonic stem cells (mESC), a relationship potentially related to redox homeostasis...
October 9, 2017: Birth defects research
https://www.readbyqxmd.com/read/28986714/comparison-of-antiretroviral-drug-resistance-among-treatment-naive-and-treated-hiv-infected-individuals-in-shiraz-iran
#20
Mohammad Ali Davarpanah, Nasrin Motazedian, Hassan Joulaei, Mohammad Reza Aghasadeghi, Hossein Faramarzi, Ehsan Aghah
The use of anti-retroviral therapy has been effective in controlling the spread of HIV-1, and has prolonged life expectancy, but this success can be affected by the emergence of drug resistance. The main goal of this study was to investigate drug resistance in the reverse transcriptase (RT), and protease (PR) genes among HIV-1 infected individuals. We systematically selected 59 HIV-1 infected individuals from Shiraz Voluntary Counseling and Testing Center (29 treatment- naïve and 30 treated). In this study intravenous drug users older than 18 were included in this study...
October 6, 2017: Archives of Virology
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