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https://www.readbyqxmd.com/read/29458000/evidence-for-a-mitochondrial-atp-regulated-potassium-channel-in-human-dermal-fibroblasts
#1
Piotr Bednarczyk, Anna Kicinska, Michal Laskowski, Bogusz Kulawiak, Rafal Kampa, Agnieszka Walewska, Milena Krajewska, Wieslawa Jarmuszkiewicz, Adam Szewczyk
Mitochondrial ATP-regulated potassium channels are present in the inner membrane of the mitochondria of various cells. In the present study, we show for the first time mitochondrial ATP-regulated potassium channels in human dermal fibroblast cells. Using the patch-clamp technique on the inner mitochondrial membrane of fibroblasts, we detected a potassium channel with a mean conductance equal to 100 pS in symmetric 150 mM KCl. The activity of this channel was inhibited by a complex of ATP/Mg2+ and activated by potassium channel openers such as diazoxide or BMS 191095...
February 16, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29456985/evaluation-of-mir-21-inhibition-and-its-impact-on-cancer-susceptibility-candidate-2-long-noncoding-rna-in-colorectal-cancer-cell-line
#2
Miganoosh Simonian, Mohammadreza Sharifi, Reza Nedaeinia, Meysam Mosallaie, Sharifeh Khosravi, Amir Avan, Majid Ghayour-Mobarhan, Hadi Bagheri, Rasoul Salehi
Background: Both microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) have been shown to have a critical role in the regulation of cellular processes such as cell growth and apoptosis, as well as cancer progression and metastasis. lncRNAs are aberrantly expressed in many diseases including cancer. Although it is well known that miRNAs can target a large number of protein-coding genes, little is known whether miRNAs can also target lncRNAs. In the present study, we determine whether miR-21 can regulate lncRNA cancer susceptibility candidate 2 (CASC2) in colorectal cancer...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29456814/ascorbic-acid-augments-colony-spreading-by-reducing-biofilm-formation-of-methicillin-resistant-staphylococcus-aureus
#3
Zulfiqar Ali Mirani, Muhammad Naseem Khan, Anila Siddiqui, Fouzia Khan, Mubashir Aziz, Shagufta Naz, Ayaz Ahmed, Seema Ismat Khan
Objectives: Staphylococcus aureus is a Gram-positive pathogen, well known for its resistance and versatile lifestyle. Under unfavourable conditions, it adapts biofilm mode of growth. For staphylococcal biofilm formation, production of extracellular polymeric substances (EPS) is a pre-requisite, which is regulated by ica operon-encoded enzymes. This study was designed to know the impact of ascorbic acid on biofilm formation and colony spreading processes of S. aureus and MRSA. Materials and Methods: The isolates of methicillin-resistant S...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29456678/association-between-dscr4-gene-methylation-in-plasma-in-early-pregnancy-and-down-s-syndrome
#4
Lingyu Hu, Changju Zhou
Down's syndrome (DS), a chromosomal abnormal genetic disease caused by a local or total copy of chromosome 21, leads to patients suffering from delayed body growth, special facies, mild to moderate mental retardation and other symptoms, seriously affecting the life of patients. The aim of the present study was to examine the association between Down's syndrome critical region 4 ( DSCR4 ) gene methylation in plasma in high-risk pregnant women with DS in early pregnancy (hereinafter referred to as pregnant women in early pregnancy) and DS, in order to screen new epigenetic markers for the clinical diagnosis of DS...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29454929/hur-mediated-scn5a-mrna-stability-reduces-arrhythmic-risk-in-heart-failure
#5
Anyu Zhou, An Xie, Tae Yun Kim, Hong Liu, Guangbin Shi, Gyeoung-Jin Kang, Ning Jiang, Man Liu, Euy-Myoung Jeong, Bum-Rak Choi, Samuel C Dudley
BACKGROUND: Downregulated sodium currents in heart failure (HF) has been linked to increased arrhythmic risk. Reduced expression of mRNA stabilizing protein ELAVL1/HuR may be responsible for the downregulation of sodium channel gene SCN5A mRNA. OBJECTIVE: To investigate whether HuR regulates SCN5A mRNA expression and whether manipulation of HuR benefits arrhythmia control in HF. METHODS: Quantitative real-time reverse-transcriptase PCR (qRT-PCR) were used to investigate the expression of SCN5A...
February 15, 2018: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/29449673/the-predictive-and-prognostic-potential-of-plasma-telomerase-reverse-transcriptase-tert-rna-in-rectal-cancer-patients
#6
Enrica Rampazzo, Paola Del Bianco, Roberta Bertorelle, Caterina Boso, Alessandro Perin, Giovanna Spiro, Francesca Bergamo, Claudio Belluco, Angela Buonadonna, Elisa Palazzari, Sara Leonardi, Antonino De Paoli, Salvatore Pucciarelli, Anita De Rossi
BACKGROUND: Preoperative chemoradiotherapy (CRT) followed by surgery is the standard care for locally advanced rectal cancer, but tumour response to CRT and disease outcome are variable. The current study aimed to investigate the effectiveness of plasma telomerase reverse transcriptase (TERT) levels in predicting tumour response and clinical outcome. METHODS: 176 rectal cancer patients were included. Plasma samples were collected at baseline (before CRT=T0), 2 weeks after CRT was initiated (T1), post-CRT and before surgery (T2), and 4-8 months after surgery (T3) time points...
February 15, 2018: British Journal of Cancer
https://www.readbyqxmd.com/read/29446977/establishment-growth-proliferation-and-gene-expression-of-buffalo-bubalus-bubalis-transgenic-fetal-fibroblasts-containing-human-insulin-gene-and-production-of-embryos-by-handmade-cloning-using-these-cells
#7
Parul Mehta, Ramakant Kaushik, Karn Pratap Singh, Ankur Sharma, Manoj Kumar Singh, Manmohan Singh Chauhan, Prabhat Palta, Suresh Kumar Singla, Radhey Sham Manik
The aim of the present study was to compare transgenic cells, containing human insulin gene kept under the control of mammary gland-specific buffalo beta-lactoglobulin promoter, and their counterparts, that is, nontransgenic cells, for examining their potential for the production of embryos following somatic cell nuclear transfer (SCNT). The gene construct was delivered into buffalo fetal fibroblasts (BFF) by nucleofection following which, the transfected cells were selected by culture in the presence of G418 for 3 weeks...
February 15, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29444701/a-case-series-on-common-cold-to-severe-bronchiolitis-and-pneumonia-in-children-following-human-metapneumovirus-infection-in-sri-lanka
#8
J A A S Jayaweera, F Noordeen, S Kothalaweala, F N N Pitchai, M L M Rayes
OBJECTIVES: The prevalence of hMPV infections in Sri Lanka has not been reported and here we report a case series of hMPV infection in children less than 5 years. Patients with ARTI were included from Teaching Hospital, Anuradhapura from March 2013 to August 2014. Indirect fluorescence assay was performed on nasopharyngeal aspirates for the identification of respiratory viruses [respiratory syncytial virus (RSV), parainfluenza virus 1, 2 and 3, influenza A and B and hMPV]. Moreover, reverse transcriptase-polymerase chain reaction was done to further confirm the hMPV infection...
February 14, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29441798/investigating-mirna-661-and-atg4-b-mrna-expression-as-potential-biomarkers-for-hepatocellular-carcinoma
#9
Mahmoud A Ali, Marwa Matboli, Nashwa El-Khazragy, Osama Saber, Sarah El-Nakeep, Hana M Abdelzaher, Ayman El-Sayed Shafei, Randa Mostafa
AIM: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. PATIENTS & METHODS: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. RESULTS: The expression of miR-661 and ATG-4B mRNA was positive in 97...
February 14, 2018: Biomarkers in Medicine
https://www.readbyqxmd.com/read/29439669/a-novel-mir-375-hoxb3-cdca3-dnmt3b-regulatory-circuitry-contributes-to-leukemogenesis-in-acute-myeloid-leukemia
#10
Laixi Bi, Bin Zhou, Haiying Li, Licai He, Chunjing Wang, Zhonggai Wang, Liqing Zhu, Mengqian Chen, Shenmeng Gao
BACKGROUND: Acute myeloid leukemia (AML) is a heterogeneous group of hematopoietic malignancies due to sophisticated genetic mutations and epigenetic dysregulation. MicroRNAs (miRNAs), a class of small non-coding RNAs, are important regulators of gene expression in all biological processes, including leukemogenesis. Recently, miR-375 has been reported to be a suppressive miRNA in multiple types of cancers, but its underlying anti-leukemia activity in AML is largely unknown. METHODS: Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure the expression of miR-375 and HOXB3 in leukemic cells and normal controls...
February 13, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29439315/downregulated-mir-217%C3%A2-expression-predicts-a-poor-outcome-in-acute%C3%A2-myeloid-leukemia
#11
Jinhua Yan, Guohe Wu, Jianlan Chen, Lifang Xiong, Guoan Chen, Ping Li
BACKGROUND: Circulating microRNAs (miRNAs) play an essential role in the development and progression of acute myeloid leukemia (AML). However, the clinical value of serum miR-217 in AML remained poorly known. OBJECTIVE: This study aimed to explore the clinical significance of serum miR-217 in AML. METHODS: Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed to detect miR-217 levels in the blood samples obtained from 89 AML patients and 60 healthy controls...
January 29, 2018: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29438976/role-of-upstream-stimulatory-factor-2-in-glutamate-dehydrogenase-gene-transcription
#12
Carlos Gaspar, Jonás I Silva-Marrero, María C Salgado, Isabel V Baanante, Isidoro Metón
Glutamate dehydrogenase (Gdh) plays a central role in ammonia detoxification by catalysing reversible oxidative deamination of L-glutamate into α-ketoglutarate using NAD+ or NADP+ as cofactor. To gain insight into transcriptional regulation of glud, the gene that codes for Gdh, we isolated and characterised the 5' flanking region of glud from gilthead sea bream (Sparus aurata). In addition, tissue distribution, the effect of starvation as well as short- and long-term refeeding on Gdh mRNA levels in the liver of S...
February 8, 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29435037/analysis-of-the-association-of-the-expression-of-kiss-1-in-colorectal-cancer-tissues-with-the-pathology-and-prognosis
#13
Xinkai Huo, Lei Zhang, Tao Li
Colorectal cancer is a common malignant tumor of the digestive tract with high morbidity and mortality rates. The aim of the present study was to examine the expression level of KiSS-1 in tumor tissues of patients with colorectal cancer, and to explore the relationship with the clinicopathology and prognosis of patients with colorectal cancer. Frozen tumor tissue and corresponding cancer-adjacent normal tissue specimens were selected from 56 patients with colorectal cancer who were treated in the Department of Surgery of our hospital from May 2009 to December 2011...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29432914/identification-of-the-heme-acquisition-system-in-vibrio-vulnificus-m2799
#14
Hiroaki Kawano, Katsushiro Miyamoto, Megumi Yasunobe, Masahiro Murata, Eri Yamahata, Ryo Yamaguchi, Yuta Miyaki, Takahiro Tsuchiya, Tomotaka Tanabe, Tatsuya Funahashi, Hiroshi Tsujibo
Vibrio vulnificus, the causative agent of serious, often fatal, infections in humans, requires iron for its pathogenesis. As such, it obtains iron via both vulnibactin and heme-mediated iron-uptake systems. In this study, we identified the heme acquisition system in V. vulnificus M2799. The nucleotide sequences of the genes encoding heme receptors HupA and HvtA and the ATP-binding cassette (ABC) transport system proteins HupB, HupC, and HupD were determined, and then used in the construction of deletion mutants developed from a Δics strain, which could not synthesize vulnibactin...
February 9, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29430376/whole-genome-resequencing-and-transcriptomic-analysis-of-genes-regulating-anthocyanin-biosynthesis-in-black-rice-plants
#15
Jae-Hyeon Oh, Ye-Ji Lee, Eun-Ju Byeon, Byeong-Chul Kang, Dong-Soo Kyeoung, Chang-Kug Kim
Anthocyanins are involved in many diverse functions in rice, but their benefits have yet to be clearly demonstrated. Our objective in this study was to identify anthocyanin-related genes in black rice plants. We identified anthocyanin-related genes in black rice plants using a combination of whole-genome resequencing, RNA-sequencing (RNA-seq), microarray experiments, and reverse-transcriptase polymerase chain reaction (RT-PCR). Using multi-layer screening from 30 rice accessions, we identified 172,922 single-nucleotide polymorphisms (SNPs) and 1276 differentially expressed genes that appear to be related to anthocyanin biosynthesis...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29429811/hospital-based-surveillance-for-rotavirus-diarrhea-in-ulaanbaatar-mongolia-april-2009-through-march-2016
#16
Altanchimeg Samdan, Sarangua Ganbold, Oyunchimeg Guntev, Solongo Orosoo, Narmandakh Javzandorj, Amar Gongor, Altansukh Enkhtuvshin, Sodbayar Demberelsuren, Wajid Abdul, Youngmee Jee, Varja Grabovac, Carl Kirkwood, Kimberley Fox, Batmunkh Nyambat
BACKGROUND: Diarrheal disease is one of the leading causes of illness and death in young children in the world, especially the developing countries. Diarrheal disease results in about half a million childhood death per year, ranking second among all causes worldwide. Diarrheal disease due to rotavirus infection is currently the most common cause of severe diarrhea in infants and young children worldwide. Rotavirus immunization of infants is a safe and effective public health intervention for rotavirus infection control and expected to lead to a reduction of childhood morbidity and mortality...
February 8, 2018: Vaccine
https://www.readbyqxmd.com/read/29429253/-diagnostic-significance-of-serum-free-dna-human-telomerase-reverse-transcriptase-quantitative-determination-on-spinal-cord-injury
#17
M K Yang, J Tang, Z Xiang, X Zhang, J Wang, Z Li, Y Li, W B Sheng
Objective: To investigate the relationship between the content of human telomerase reverse transcriptase (hTERT) and its clinical features in serum free DNA in patients with different degree of spinal cord injury. Methods: From December 2013 to December 2016, inpatients of the Central Hospital of Bazhong, Sichuan Province were enrolledand divided into the experimental group, the disease control group and the negative control group. For the experimental group: 46 patients with spinal cord injury were graded according to the criteria of the American Association of Spinal Cord Injury (ASIA), including 12 cases of grade A, 10 cases of grade B, 10 cases of grade C, 7 cases of grade D and 7 cases of grade E; for the disease control group: 15 patients with spinal fractures (without spinal cord injury) at the same period were included; and for the negative control group: 20 healthy adult volunteers aged 18-50 years were selected...
February 6, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29426696/prognostic-impact-of-concomitant-loss-of-pbrm1-and-bap1-protein-expression-in-early-stages-of-clear-cell-renal-cell-carcinoma
#18
Walter Henriques da Costa, Isabela Werneck da Cunha, Aline Fusco Fares, Stephania M Bezerra, Luciana Shultz, Diego Abreu Clavijo, Deusdedit Vieira da Silva, George Jabboure Netto, Gustavo Cardoso Guimaraes, Stenio de Cassio Zequi
PURPOSE: To evaluate the prognostic impact of immunohistochemical expression of BAP1 and PBRM1 in patients with early stage (pT1-pT2N0M0) clear cell renal cell carcinoma (ccRCC). PATIENTS AND METHODS: A total of 441 consecutive patients treated surgically for stages I and II (TNM-AJCC 2010) ccRCC between 1990 and 2016 were selected. All cases were reviewed for uniform reclassification and the most representative tumor areas were selected for the construction of a tissue microarray...
February 6, 2018: Urologic Oncology
https://www.readbyqxmd.com/read/29426367/pip5k1%C3%AE-promotes-myogenic-differentiation-via-akt-activation-and-calcium-release
#19
Xiaofan Chen, Jun Wan, Bo Yu, Yarui Diao, Wei Zhang
BACKGROUND: Skeletal muscle satellite cell-derived myoblasts are mainly responsible for postnatal muscle growth and injury-induced regeneration. Many intracellular signaling pathways are essential for myogenic differentiation, while a number of kinases are involved in this modulation process. Type I phosphatidylinositol 4-phosphate 5-kinase (PIP5KI) was identified as one of the key kinases involved in myogenic differentiation, but the underlying molecular mechanism is still unclear. METHODS: PIP5K1α was quantified by quantitative reverse transcriptase PCR and western blot assay...
February 9, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29424435/diagnostic-performance-of-the-sofia%C3%A2-influenza-a%C3%A2-%C3%A2-b-fluorescent-immunoassay-in-adult-outpatients-in-northern-taiwan
#20
Jeng-How Yang, Po-Yen Huang, Shian-Sen Shie, Shuan Yang, Kuo-Chien Tsao, Tsu-Lan Wu, Ting-Shu Wu, Ching-Tai Huang
To evaluate the diagnostic performance of the Sofia influenza A + B fluorescent immunoassay (Sofia FIA), we performed a prospective study at the Chang Gung Memorial Hospital in Taiwan from January 2012 to December 2013. Patients who presented at out-patient clinics or the emergency department with influenza-like illness were included. Upper respiratory tract specimens were collected from oropharynx or nasopharynx. Performance of the Sofia FIA was compared to that of the Formosa One Sure Flu A/B Rapid Test...
February 9, 2018: Journal of Medical Virology
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