keyword
MENU ▼
Read by QxMD icon Read
search

Reverse transcriptase pcr

keyword
https://www.readbyqxmd.com/read/29239061/progesterone-inhibited-endoplasmic-reticulum-stress-associated-apoptosis-induced-by-interleukin-1%C3%AE-via-the-grp78-perk-chop-pathway-in-bewo-cells
#1
Ling Liu, Yan Zhang, Yankui Wang, Wei Peng, Ning Zhang, Yuanhua Ye
AIM: Pre-eclampsia (PE) is a pregnancy complication characterized by new onset maternal hypertension and proteinuria. Its underlying mechanisms are unclear. This study investigated the relationship between progesterone and endoplasmic reticulum stress (ERS) associated apoptosis induced by interleukin (IL)-1β via the glucose regulated protein 78 (GRP78)/protein kinase RNA-like endoplasmic reticulum kinase (PERK)/C/EBP-homologous protein (CHOP) pathway in BeWo cells. METHODS: Venous blood and placental tissues were collected from PE patients, normal pregnancy and preterm delivery cases, respectively...
December 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29238201/diagnostic-significance-of-circulating-long-noncoding-rna-pcat6-in-patients-with-non-small-cell-lung-cancer
#2
Li Wan, Lin Zhang, Kai Fan, Jian-Jun Wang
Aim: We have previously shown that the long noncoding RNA prostate cancer-associated transcript 6 (PCAT6) promoted the proliferation and invasion of lung adenocarcinoma (LUAD) cells. In this study, the diagnostic significance of tissue and serum PCAT6 was evaluated in non-small cell lung cancer (NSCLC). Materials and methods: Tissue expression of PCAT6 was systematically evaluated in five Gene Expression Omnibus datasets (GSE19804, GSE18842, GSE30219, GSE19188, and GSE27262)...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29237907/long-noncoding-rna-activated-by-transforming-growth-factor-%C3%AE-promotes-cancer-development-and-is-a-prognostic-marker-in-cervical-cancer
#3
Weichun Cao, Tianfang Peng, Yi Zhou
BACKGROUND: Long noncoding RNAs (lncRNAs) have been highlighted as crucial elements in cancer biology. LncRNA activated by transforming growth factor-β (ATB) was identified to promote the development of multiple cancers and may serve as a potential therapeutic target in human cancers. However, to the best of our knowledge, the functional role of ATB in cervical cancer has not been verified yet. MATERIALS AND METHODS: The expression of lncRNA ATB was evaluated by quantitative reverse transcriptase-polymerase chain reaction assay in cervical cancer cell lines and clinical specimens...
2017: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/29235695/enzymatic-characterization-of-two-epsilon-class-glutathione-s-transferases-of-spodoptera-litura
#4
Aiko Hirowatari, Zhiwei Chen, Kazuei Mita, Kohji Yamamoto
Two cDNAs encoding glutathione S-transferase (GST) of the tobacco cutworm, Spodoptera litura, were cloned by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequences of the resulting clones revealed 32-51% identities to the epsilon-class GSTs from other organisms. The recombinant proteins were functionally overexpressed in Escherichia coli cells in soluble form and were purified to homogeneity. The enzymes were capable of catalyzing the bioconjugation of glutathione with 1-chloro-2,4-dinitrobenzene, 1,2-epoxy-3-(4-nitrophenoxy)-propane, and ethacrynic acid...
December 13, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/29229458/the-effects-of-irrigants-on-the-survival-of-human-stem-cells-of-the-apical-papilla-including-endocyn
#5
Mark B Scott, Gregory S Zilinski, Timothy C Kirkpatrick, Van T Himel, Kent A Sabey, Thomas E Lallier
INTRODUCTION: Endocyn, a pH-neutral solution of hypochlorous acid and hypochlorite has been developed for use as an endodontic irrigant. The purpose of this study was to evaluate the effect of Endocyn on human periodontal ligament (PDL) fibroblasts, rat osteosarcoma cells (UMR-106), and stem cells of the apical papilla (SCAP) compared with other commonly used endodontic irrigants. METHODS: To determine cytotoxicity, cells were exposed to various concentrations of Endocyn, 6% sodium hypochlorite (NaOCl), 17% EDTA, and 2% chlorhexidine for 10 minutes, 1 hour, or 24 hours...
December 8, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29228527/synergistic-effects-of-resistin-and-visfatin-as-adipocyte-derived-hormones-on-telomerase-gene-expression-in-ags-gastric-cancer-cell-line
#6
Masoumeh Mohammadi, Nosratollah Zarghami, Mehdi Hedayati, Sara Ghaemmaghami
Recently suggested that adipocytokines may play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. The previous study showed Resistin and Visfatin, as adipocyte derived hormones, separately increases telomerase (hTERT) gene, the aim of this study is investigating synergic effects of Resistin and Visfatin on telomerase gene expression, in AGS gastric cancer cell line. In this study, human gastric cancer AGS cell line was selected. After stimulation with increasing concentrations of Resistin and Visfatin recombinant proteins for 24 and 48 hours, cell proliferation was assessed by XTT assay...
October 2017: Acta Medica Iranica
https://www.readbyqxmd.com/read/29225515/anticancer-activity-of-astragalus-polysaccharide-in-human-non-small-cell-lung-cancer-cells
#7
Chao-Yan Wu, Yuan Ke, Yi-Fei Zeng, Ying-Wen Zhang, Hai-Jun Yu
Background: We have reported that Chinese herbs Astragalus polysaccharide (APS) can inhibit nuclear factor kappaB (NF-κB) activity during the development of diabetic nephropathy in mice. NF-κB plays important roles in genesis, growth, development and metastasis of cancer. NF-κB is also involved in the development of treatment resistance in tumors. Here we investigated the antitumor activity of APS in human non-small cell lung cells (A549 and NCI-H358) and the related mechanisms of action...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/29224880/the-effect-of-different-treatments-for-early-lactation-hyperketonemia-on-liver-triglycerides-glycogen-and-expression-of-key-metabolic-enzymes-in-dairy-cattle
#8
S Mann, F A Leal Yepes, J J Wakshlag, E Behling-Kelly, J A A McArt
Despite the widespread use of treatments for postpartum hyperketonemia in dairy cows, there is currently a lack of evidence comparing their effects on both the resolution of hyperketonemia and the potential effects on the liver of affected animals. The objective of our work was to investigate the effect of commonly used hyperketonemia treatments on hepatic triglyceride and glycogen content as well as on the mRNA and protein abundance of key enzymes involved in gluconeogenesis, ketogenesis, and lipid metabolism...
December 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29224320/-comparative-study-of-cytogenetic-response-evaluated-by-conventional-banding-analysis-and-fluorescence-in-situ-hybridization-in-chronic-myeloid-leukemia-patients-during-tyrosine-kinase-inhibitor-treatment
#9
Z Wang, N Li, L Gao, L Feng, Y Z Qin, H Dang, Y Shi, Q He, Q Jiang, H Jiang, Y Y Lai
Objective: To compare the cytogenetic response detected by conventional banding analysis (CBA) and fluorescence in situ hybridization (FISH) and to explore the correlation between the cytogenetic and molecular response in chronic myeloid leukemia (CML) patients during tyrosine kinase inhibitor (TKI) treatment. Methods: CBA, FISH and real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) methods were performed to detect the cytogenetic and molecular response simultaneously in 504 bone marrow samples from 367 CML patients who received TKI treatment...
November 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/29223538/curcumin-induces-osteoblast-differentiation-through-mild-endoplasmic-reticulum-stress-mediated-such-as-bmp2-on-osteoblast-cells
#10
Hyo-Eun Son, Eun-Jung Kim, Won-Gu Jang
AIMS: Curcumin (diferuloylmethane or [1E,6E]-1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6heptadiene-3,5-dione) is a phenolic natural product derived from the rhizomes of the turmeric plant, Curcuma longa. It is reported to have various biological actions such as anti-oxidative, anti-inflammatory, and anti-cancer effects. However, the molecular mechanism of osteoblast differentiation by curcumin has not yet been reported. MAIN METHODS: The cytotoxicity of curcumin was identified using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
December 6, 2017: Life Sciences
https://www.readbyqxmd.com/read/29223481/can-breast-cancer-patients-with-her2-dual-equivocal-tumours-be-managed-as-her2-negative-disease
#11
Yiwei Tong, Xiaosong Chen, Xiaochun Fei, Lin Lin, Jiayi Wu, Ou Huang, Jianrong He, Li Zhu, Weiguo Chen, Yafen Li, Kunwei Shen
BACKGROUND: Increasing human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC)/fluorescence in situ hybridisation (FISH) dual-equivocal breast tumours are reported after the 2013 American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guideline update. The aim of this study is to investigate the clinico-pathologic characteristics, treatment patterns and disease outcome of these patients with HER2 dual-equivocal tumours. PATIENTS AND METHODS: Patients with HER2 IHC 2+ and available FISH results were retrospectively analysed from the Comprehensive Breast Health Center, Shanghai Ruijin Hospital...
December 7, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/29221474/increased-expression-of-neuregulin-1-in-the-urothelium-of-rat-bladder-with-partial-bladder-outlet-obstruction
#12
Seung Woo Yang, Seong Woo Jeong, Ki Hak Song
BACKGROUND: This study determined whether changes in the expression of neuregulin (NRG) 1, erbB2 tyrosine kinase (ErbB2) and the M2 muscarinic receptor in the urothelium and detrusor muscle of the rat bladder were associated with partial bladder outlet obstruction (PBOO). METHODS: Male Sprague-Dawley rats (body weight 250-300 g) were used and subdivided into control (n = 10) and PBOO groups (n = 20). PBOO was induced for 21 days, and the expression of NRG1, ErbB2 and M2 muscarinic receptor mRNA and protein was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting, respectively...
December 8, 2017: BMC Urology
https://www.readbyqxmd.com/read/29219024/excess-maternal-thyroxine-alters-the-proliferative-activity-and-angiogenic-profile-of-growth-cartilage-of-rats-at-birth-and-weaning
#13
Lorena Gabriela Rocha Ribeiro, Juneo Freitas Silva, Natália de Melo Ocarino, Cíntia Almeida de Souza, Eliane Gonçalves de Melo, Rogéria Serakides
Objective The aim of this study was to unravel the mechanisms by which thyroxine affects skeletal growth by evaluating proliferative activity and angiogenic profile of growth cartilage of neonatal and weanling rats. Methods Sixteen adult Wistar rats were equally divided into 2 groups: control and treated with thyroxine during pregnancy and lactation. The weight, measurement of plasma free T4 and thyroids, femurs' histomorphometric analysis, and proliferative activity and angiogenic profile by immunohistochemical or real-time reverse transcriptase-polymerase chain reaction in growth cartilage was performed...
January 2018: Cartilage
https://www.readbyqxmd.com/read/29218084/regulation-of-osteogenic-differentiation-by-dna-methylation-of-the-dishevelled-gene-in-bone-marrow-mesenchymal-stem-cells
#14
Xiaofeng Han, Xinfeng Li, Guibin Zhong, Zude Liu
Bone marrow mesenchymal stem cells (BMSCs) are stem cells with multidirectional differentiation potential, which can be used as seed cells to repair and reconstruct many types of tissues and organs following injury or disease. Osteogenic differentiation involves a variety of pathway and factors, including cytokines, growth factors, and hormones. In the present study, we investigated the potential role of Dishevelled in osteogenic differentiation of BMSCs in induction medium containing the methyltransferase inhibitor 5-aza-2'-deoxycytidine...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29218017/six2-plays-an-intrinsic-role-in-regulating-proliferation-of-mesenchymal-cells-in-the-developing-palate
#15
Dennis O Okello, Paul P R Iyyanar, William M Kulyk, Tara M Smith, Scott Lozanoff, Shaoping Ji, Adil J Nazarali
Cleft palate is a common congenital abnormality that results from defective secondary palate (SP) formation. The Sine oculis-related homeobox 2 (Six2) gene has been linked to abnormalities of craniofacial and kidney development. Our current study examined, for the first time, the specific role of Six2 in embryonic mouse SP development. Six2 mRNA and protein expression were identified in the palatal shelves from embryonic days (E)12.5 to E15.5, with peak levels during early stages of palatal shelf outgrowth...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29211240/natural-environmental-water-sources-in-endemic-regions-of-northeastern-brazil-are-potential-reservoirs-of-viable-mycobacterium-leprae
#16
Maria Luisa Bezerra de Macedo Arraes, Maísa Viana de Holanda, Luana Nepomuceno Gondim Costa Lima, José Antônio Beltrão Sabadia, Cynthia Romariz Duarte, Rosa Livia Freitas Almeida, Carl Kendall, Ligia Regina Sansigolo Kerr, Cristiane Cunha Frota
BACKGROUND: The detection of live Mycobacterium leprae in soil and animals other than humans suggests that the environment plays a role in the transmission of leprosy. OBJECTIVE: The objective of this study was to investigate the presence of viable M. leprae in natural water sources used by the local population in five municipalities in the state of Ceará, northeastern Brazil. METHODS: Samples were collected from 30 different sources. Viable bacilli were identified by reverse transcriptase polymerase chain reaction (PCR) of the M...
December 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29209126/intestinal-parameters-of-oxidative-imbalance-in-celiac-adults-with-extraintestinal-manifestations
#17
Agnieszka Piatek-Guziewicz, Agata Ptak-Belowska, Magdalena Przybylska-Felus, Pawel Pasko, Pawel Zagrodzki, Tomasz Brzozowski, Tomasz Mach, Malgorzata Zwolinska-Wcislo
AIM: To evaluate selected intestinal parameters of oxidative stress, and antioxidant capacity in adult celiac disease patients with extraintestinal manifestations. METHODS: The study involved 85 adult patients divided into the following subgroups: (1) patients with newly diagnosed celiac disease (CD) (n = 7); (2) celiac patients not adhering to a gluten-free diet (GFD) (n = 22); (3) patients with CD on the GFD (n = 31); and (4) patients with functional disorders of the gastrointestinal tract, serving as controls (n = 25)...
November 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29208044/an-outbreak-of-acute-haemorrhagic-conjunctivitis-associated-with-coxsackievirus-a24-variant-in-the-gambia-west-africa
#18
Sarah E Burr, Ansumana Sillah, Hassan Joof, Robin L Bailey, Martin J Holland
OBJECTIVE: An outbreak of acute haemorrhagic conjunctivitis occurred in The Gambia, West Africa in 2011. Affected individuals presented with conjunctival haemorrhages, swelling and ocular discharge. In an effort to identify a causative agent of the disease, ocular swabs were taken from patients during the acute and convalescent phases. Total RNA was extracted from all samples and reverse-transcriptase PCR performed using primers specific for all enteroviruses. Resulting amplicons were sequenced and data compared to known sequences using the BLAST algorithm...
December 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29205397/effects-of-long-term-alcohol-drinking-on-the-dopamine-d2-receptor-gene-expression-and-heteroreceptor-complexes-in-the-striatum-in-rats
#19
Kristin Feltmann, Dasiel Oscar Borroto-Escuela, Joëlle Rüegg, Luca Pinton, Thatiane de Oliveira Sergio, Manuel Narváez, Antonio Jimenez-Beristain, Tomas J Ekström, Kjell Fuxe, Pia Steensland
BACKGROUND: Reduced dopamine D2 receptor (D2R) ligand binding has repeatedly been demonstrated in the striatum of humans with alcohol use disorder (AUD). The attenuated D2R binding has been suggested to reflect a reduced D2R density, which in turn has been proposed to drive craving and relapse. However, results from rodent studies addressing the effects of alcohol drinking on D2R density have been inconsistent. METHODS: A validated alcohol drinking model (intermittent access to 20% alcohol) in Wistar rats was used to study the effects of voluntary alcohol drinking (at least 12 weeks) on the D2R in the striatum compared to age-matched alcohol-naïve control rats...
December 5, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29204990/apoptosis-induction-of-colorectal-cancer-cells-htl-9-in-vitro-by-the-transformed-products-of-soybean-isoflavones-by-ganoderma-lucidum
#20
Mei-Lin Cui, Huan-Yi Yang, Guo-Qing He
Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of Ganoderma lucidum. The resultant transformed products (TSI) contained (703.21±4.35) mg/g of genistein, with transformed rates of 96.63% and 87.82% of daidzein and genistein, respectively, and TSI also could enrich the bioactive metabolites of G. lucidum. The antitumor effects of TSI on human colorectal cancer cell line HTL-9, human breast cancer cell line MCF-7, and human immortalized gastric epithelial cell line GES-1 were also studied...
2017: Journal of Zhejiang University. Science. B
keyword
keyword
53719
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"