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https://www.readbyqxmd.com/read/29667206/surveillance-of-donated-blood-during-the-2016-arbovirus-outbreak-in-brazil
#1
Rohit Sharma, Larissa Costa Santos, Robson Amaro da Silva, Caroline Vieira Gonçalves, Stéfano de Melo Calado, Denisar P Santos, Josana Passos de Andrade de Melo, Rita de Cássia Pontello Rampazzo, Luciana Requião, Marco Aurélio Krieger, Manoel Barral-Netto, Camila I de Oliveira
Reports of arboviral transmission via blood transfusion may be a cause of concern among asymptomatic infected donors. This study evaluated the presence of arboviruses in donated blood products during the 2016 outbreak in Vitória da Conquista (Bahia-Brazil). Serum samples (n = 676) were screened for ZIKV, CHIKV and the four DENV serotypes using a one-step reverse transcriptase-based Real-Time Polymerase Chain Reaction (RT-PCR). No samples tested positive for any of the targets, whereas positive controls performed as expected...
April 17, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29666658/phylogenetic-analysis-of-dengue-virus-in-bangkalan-madura-island-east-java-province-indonesia
#2
Teguh Hari Sucipto, Tomohiro Kotaki, Kris Cahyo Mulyatno, Siti Churrotin, Amaliah Labiqah, Soegeng Soegijanto, Masanori Kameoka
Dengue virus (DENV) infection is a major health issue in tropical and subtropical areas. Indonesia is one of the biggest dengue endemic countries in the world. In the present study, the phylogenetic analysis of DENV in Bangkalan, Madura Island, Indonesia, was performed in order to obtain a clearer understanding of its dynamics in this country. A total of 359 blood samples from dengue-suspected patients were collected between 2012 and 2014. Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29665312/interleukin-13-associates-with-life-threatening-rhinovirus-infections-in-infants-and-young-children
#3
Mauricio T Caballero, Diego R Hijano, Patricio L Acosta, Cecilia G Mateu, Débora N Marcone, Jodell E Linder, Laura B Talarico, John M Elder, Marcela Echavarria, Eva Kathryn Miller, Fernando P Polack
OBJECTIVE: Delineate risk factors associated with severe hypoxemia (O2 sat ≤87%) in infants and children younger than 2 years hospitalized with single pathogen HRV infection. STUDY DESIGN: Prospective study in a yearly catchment population of 56 560 children <2 years old between 2011 and 2013 in Argentina. All children with respiratory signs and O2 sat <93% on admission were included. HRV infections were identified by reverse transcriptase-polymerase chain reaction...
April 17, 2018: Pediatric Pulmonology
https://www.readbyqxmd.com/read/29663998/-microrna-34a-participates-in-lipopolysaccharide-mediated-sepsis-related-renal-function-impairment-via-kruppel-like-factor-4
#4
Qidong Jiang, Changxue Wu, Qiong Zhang
OBJECTIVE: To investigate whether microRNA-34a (miR-34a) participates in lipopolysaccharide (LPS) mediated sepsis related renal function impairment via Kruppel-like factor 4 (KLF4). METHODS: Thirty healthy male Sprague-Dawley (SD) rats, weighing 180-200 g, were randomly divided into two groups: control group and model group, with 15 rats in each group. The SD rats from model group were injected with LPS 7.5 mg/kg to induce sepsis related renal function impairment model, the SD rats from control group were injected with normal saline...
April 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29663899/hiv-1-drug-resistance-profiles-for-the-hiv-protease-and-reverse-transcriptase-gene-in-patients-receiving-combination-therapy-in-tehran-iran
#5
Leila Sadeghi, Mahin Lolaie, Reza Adl Tabatabai, Saeed Bayanolhagh, Leila Taj, Niloofar Eghbal Ahmadi, Negin Abedinzadeh, Farzaneh Vakili, Ghazaleh Ahmadi, Minoo Mohraz
BACKGROUND: Determination of drug resistant mutations has a crucial role in the management of HIV-1 infected patients. OBJECTIVE: The aim of the current study was to evaluate drug resistance profile of RT and PR gene, and find the correlation between drug resistance mutation and ART regimen to intensify physicians ' options for the most effective therapy and help to establish health related policies at the national level in Iran. METHOD: HIV-1 RNA of 34 samples was extracted from plasma and RT Nested- PCR was performed and the final products were sequenced...
April 15, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29661824/development-of-dengue-virus-serotype-specific-ns1-capture-assays-for-the-rapid-and-highly-sensitive-identification-of-the-infecting-serotype-in-human-sera
#6
Katharina Röltgen, Natalie Rose, Alessia Ruggieri, Louisa Warryn, Nicole Scherr, Carlos Augusto Pinho-Nascimento, Marco Tamborrini, Thomas Jaenisch, Gerd Pluschke
Dengue fever can be caused by one of four distinct dengue virus (DENV) serotypes that cocirculate in many parts of the world. Point of care serotype-specific nonstructural protein-1 (NS1) capture assays for the rapid serotyping of DENV in human sera would greatly support epidemiological surveillance and potentially also prognosis in individual patients. To ensure both serotype specificity and broad coverage of variants within serotypes, we have applied an innovative approach for the generation and selection of serotype-specific anti-NS1 mAbs...
April 16, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29659961/multi-copper-ferroxidase-deficient-mice-have-increased-brain-iron-concentrations-and-learning-and-memory-deficits
#7
Jiashuo Zheng, Ruiwei Jiang, Min Chen, Zaitunamu Maimaitiming, Junzhuo Wang, Gregory J Anderson, Chris D Vulpe, Joshua L Dunaief, Huijun Chen
Background: The accumulation of iron occurs in the central nervous system (CNS) in several neurodegenerative diseases. Although multi-copper ferroxidases (MCFs) play an important role in cellular iron metabolism and homeostasis, the mechanism of MCFs in the CNS remains unclear. Objective: The aim was to study the role of MCFs in CNS iron metabolism and homeostasis by using hephaestin/ceruloplasmin (Heph/Cp) double knockout (KO) mice. Methods: Heph/Cp double KO male mice were generated by crossing both single KO mice...
April 1, 2018: Journal of Nutrition
https://www.readbyqxmd.com/read/29656515/comparative-cyp-omic-analysis-between-the-ddt-susceptible-and-resistant-drosophila-melanogaster-strains-91-c-and-91-r
#8
Keon Mook Seong, Brad S Coates, May R Berenbaum, John M Clark, Barry R Pittendrigh
BACKGROUND: Cytochrome P450 monooxygenases (P450s) are involved in the biosynthesis of endogenous intracellular compounds and the metabolism of xenobiotics, including chemical insecticides. We investigated the structural and expression level variance across all P450 genes with respect to the evolution of insecticide resistance under multigenerational DDT selection. RESULTS: RNA-seq and reverse transcriptase quantitative PCR indicated that the transcript levels of seven P450 genes were significantly up-regulated and three P450 genes were down-regulated in the DDT-resistant strain 91-R, as compared to the control strain 91-C...
April 15, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29655629/intramuscular-vaccination-of-guinea-pigs-with-the-live-attenuated-human-herpes-simplex-vaccine-vc2-stimulates-a-transcriptional-profile-of-vaginal-th17-and-regulatory-tr1-responses
#9
Brent A Stanfield, Paul J F Rider, John Caskey, Fabio Del Piero, Konstantin G Kousoulas
Herpes simplex virus is a common causative agent of oral and genital diseases. Novel vaccines and therapeutics are needed to combat herpes infections especially after the failure of subunit vaccines in human clinical trials. We have shown that the live-attenuated HSV-1 VC2 vaccine strain is unable to establish latency in vaccinated animals and produces a robust immune response capable of completely protecting mice against lethal vaginal HSV-1 or HSV-2 infections. The guinea pig represents the best small animal model of genital HSV-2 disease...
April 11, 2018: Vaccine
https://www.readbyqxmd.com/read/29653150/validation-of-reference-genes-for-quantitative-gene-expression-analysis-in-ralstonia-pseudosolanacearum-cqps-1-under-environment-stress
#10
Liang Yang, Juanni Chen, Ying Liu, Shuting Zhang, Shili Li, Wei Ding
Quantitative real-time reverse transcriptase PCR (qRT-PCR) has become the method choice for quantification of gene expression changes, however, the accuracy of the method depends on the stability of reference genes. Ralstonia pseudosolanacearum (R. pseudosolanacearum) is an important plant pathogen, infecting >450 plant species and causing bacterial wilt. In order to identify stable reference genes in R. pseudosolanacearum CQPS-1 under different environment stresses. We used five tools (△Ct method, GeNorm, NormFinder, BestKeeper, and RefFinder) to evaluate the stability of seven candidate reference genes including phosphoglycerate kinase (PGK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 16S ribosomal RNA (16S), cell division protein ftsZ (ftsZ), DNA gyrase subunit A (gyrA), Ribosomal protein L13 (rplM), and phosphoserine aminotransferase (serC) under biotic (growth phases) and abiotic stress (temperature, hydroxycoumarins, nutrition)...
April 10, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29652029/evaluation-of-a-modified-rat-model-for-functional-dyspepsia
#11
Qiankun Liang, Yuan Yan, Lanfang Mao, Xiaojuan Du, Jingjing Liang, Junhong Liu, Longde Wang, Hongfang Li
Background/Aim: The purpose of this study was to establish a modified rat model with functional dyspepsia (FD) and analyze the changes in gastrointestinal motility and brain-gut peptide levels in serum and brain-gut axis. Materials and Methods: Male Wistar rats were divided into control group (Con) and FD model group. FD model was established by stimulating semi-starvation rats via tail damping, provocation, and forced exercise fatigue until gastrointestinal motility disorder appeared, and then levels of motilin, leptin, cholecystokinin (CCK), and vasoactive intestinal peptide (VIP) were detected in serum by enzyme linked immunosorbent assay and in duodenum, antrum, and hypothalamus by immunohistochemistry, reverse transcriptase-polymerase chain reaction, and Western blot...
April 13, 2018: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/29650441/clinical-implications-of-tert-promoter-mutation-on-idh-mutation-and-mgmt-promoter-methylation-in-diffuse-gliomas
#12
Hyun Sik Kim, Mi Jung Kwon, Joon Ho Song, Eun Soo Kim, Ho Young Kim, Kyueng-Whan Min
IDH mutation and MGMT promoter methylation are reliable prognostic and predictive biomarkers in grade II-IV diffuse gliomas. Recurrent mutations in the promoter region of the telomerase reverse transcriptase (TERTp) gene have also been found in diffuse gliomas. However, the prognostic and predictive effects of TERTp mutation on IDH or MGMT status are largely unknown. IDH1/2 and TERTp mutations, as well as MGMT methylation statuses, were examined via peptide nucleic acid-mediated PCR clamping and MGMT methylation-specific PCR in 67 paraffinized tumor samples, respectively...
April 5, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29649984/bb-cl-amidine-as-a-novel-therapeutic-for-canine-and-feline-mammary-cancer-via-activation-of-the-endoplasmic-reticulum-stress-pathway
#13
Melissa M Ledet, Robyn Anderson, Rebecca Harman, Aaron Muth, Paul R Thompson, Scott A Coonrod, Gerlinde R Van de Walle
BACKGROUND: Mammary cancer is highly prevalent in dogs and cats and results in a poor prognosis due to critically lacking viable treatment options. Recent human and mouse studies have suggested that inhibiting peptidyl arginine deiminase enzymes (PAD) may be a novel breast cancer therapy. Based on the similarities between human breast cancer and mammary cancer in dogs and cats, we hypothesized that PAD inhibitors would also be an effective treatment for mammary cancer in these animals...
April 12, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29644902/interleukin-il-36-gamma-induces-mucin-5ac-oligomeric-mucus-gel-forming-expression-via-il-36-receptor-extracellular-signal-regulated-kinase-1-and-2-and-p38-nuclear-factor-kappa-light-chain-enhancer-of-activated-b-cells-in-human-airway-epithelial-cells
#14
Chang Hoon Bae, Yoon Seok Choi, Hyung Gyun Na, Si-Youn Song, Yong-Dae Kim
BACKGROUND: Mucin 5AC, oligomeric mucus/gel-forming (MUC5AC) expression is significantly increased in allergic and inflammatory airway diseases. Interleukin (IL) 36 gamma is predominantly expressed in airway epithelial cells and plays an important role in innate and adaptive immune responses. IL-36 gamma is induced by many inflammatory mediators, including cytokines and bacterial and viral infections. However, the association between IL-36 gamma and mucin secretion in human airway epithelial cells has not yet been fully investigated...
March 2018: American Journal of Rhinology & Allergy
https://www.readbyqxmd.com/read/29644165/treatment-of-stress-urinary-incontinence-with-low-intensity-extracorporeal-shock-wave-therapy-in-a-vaginal-balloon-dilation-induced-rat-model
#15
Alex K Wu, Xiaoyu Zhang, Jianwen Wang, Hongxiu Ning, Uwais Zaid, Jaqueline D Villalta, Guifang Wang, Lia Banie, Guiting Lin, Tom F Lue
Background: To investigate the outcomes and mechanisms of low-intensity extracorporeal shock wave therapy (Li-ESWT) on stress urinary incontinence (SUI) in a vaginal balloon dilation (VBD) rat model. Methods: Thirty Sprague-Dawley rats were randomly grouped into normal controls, VBD only, and VBD with Li-ESWT. Li-ESWT was administered twice per week for 3 weeks. Afterward, all 30 rats were assessed with functional and histological studies. To explore the acute effect of Li-ESWT, another 25 rats, given intraperitoneal 5-ethynyl-2-deoxyuridine (EdU) at birth, were treated with Li-ESWT followed by assessment of vascular endothelial growth factor (VEGF) expression and endogenous progenitor cells distribution at 24 hours or 1 week after the last Li-ESWT therapy...
March 2018: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29628632/expression-of-bax-and-bcl2-genes-in-peripheral-blood-lymphocytes-of-patients-with-differentiated-thyroid-cancer
#16
Zari Hamivand, Gholamhassan Haddadi, Reza Fardid
Context: Thyroid cancer is the most common endocrine malignancy worldwide. Iodine-131 is used in the treatment of thyroid cancer with dosage of 100 mCi. In the medical applications of ionizing radiation besides the advantages such as diagnosis and treatment of diseases, the risks arising from exposure should be considered as well. Aims: The present study aimed to evaluate the changes in expression levels of apoptotic Bax and Bcl-2 and the ratio of Bax/Bcl-2, in the peripheral blood lymphocytes (PBLs) of patients with differentiated thyroid cancer (DTC)...
January 2018: Journal of Medical Physics
https://www.readbyqxmd.com/read/29626523/expression-of-genes-in-primo-vasculature-floating-in-lymphatic-endothelium-under-lipopolysaccharide-and-acupuncture-electric-stimulation
#17
Jun-Young Shin, Jong-Ok Ji, Da-Woon Choi, Sang-Heon Choi, Jong-Gu Choi, Min-Suk Rho, Ji Yoon Lee, Sang-Suk Lee
It is known that the existence of primo vascular system (PVS) including the primo nodes and vessels. However, it is poorly understood the relevant genes in the PVS system both patho- and physiologic condition. Here, we first examined the gene expression in primo vessels (PV) floating in lymphatic endothelium by isolation of PVS and lymphatic vessels (LV) containing PVS. To investigate therapeutic effects, both PV and LV containing PVS were isolated after lipopolysaccharides (LPS) injection and acupuncture electric stimulation (AES) at two acupoints of Joksamni (ST36) and Hapgok (LI04) following LPS...
April 4, 2018: Journal of Acupuncture and Meridian Studies
https://www.readbyqxmd.com/read/29624820/escherichia-coli-cellular-responses-to-exposure-to-atmospheric-pressure-dielectric-barrier-discharge-dbd-plasma-treated-n-acetylcysteine-nac-solution
#18
Utku K Ercan, Bhaswati Sen, Ari D Brooks, Suresh G Joshi
AIM: To understand the underlying cellular mechanisms during inactivation of Escherichia coli in response to antimicrobial solution of nonthermal plasma-activated N-acetylcysteine (NAC). METHODS AND RESULTS: The recommended techniques were used to demonstrate E. coli cellular and transcriptomic changes caused associated with peroxynitrite and compared with plasma-treated NAC solution. The findings demonstrate that E. coli cells respond to plasma-treated NAC, and undergo severe oxidative and nitrosative stress, and leading to stress-induced damages to different components of bacterial cells, which includes loss of membrane potential, formation of oxidized glutathione (GSSG), formation of nitro-tyrosine (a known marker of nitrosative stress), DNA damage, and generated a prominent pool of peroxynitrite...
April 6, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29622860/laboratory-monitoring-of-chronic-myeloid-leukemia-in-patients-on-tyrosine-kinase-inhibitors
#19
REVIEW
Richa Chauhan, Sudha Sazawal, H P Pati
Chronic Myeloid Leukemia (CML) is a myeloproliferative neoplasm characterized by translocation of genetic material from chromosome 9 to chromosome 22 to form a fusion gene (BCR-ABL1) that is responsible for abnormal tyrosine kinase activity and alteration of various downstream signaling pathways. In addition to morphological diagnosis of CML phase, it is essential to detect BCR-ABL1 fusion by either metaphase cytogenetics or reverse transcriptase polymerase chain reaction that also determines type of mRNA transcript...
April 2018: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29620470/study-of-an-outbreak-of-highly-pathogenic-avian-influenza-h5n8-in-commercial-pekin-ducks-anas-platyrhynchos-domesticus-in-california
#20
Simone Stoute, Beate Crossley, H L Shivaprasad
A February 2015 outbreak of highly pathogenic avian influenza (HPAI) H5N8 in a flock of commercial Pekin ducks ( Anas platyrhynchos domesticus) in California was investigated in detail. Approximately 17,349 five-wk-old ducks experienced an increased mortality from a normal of eight birds per day to 24, 18, 24, 33, and 61 birds per day, respectively, in the last 5 days prior to flock depopulation. Clinically, there was decreased water and feed consumption, and approximately 1.0% of the affected flock exhibited neurologic signs...
March 2018: Avian Diseases
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