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Purification his tagged protien

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https://www.readbyqxmd.com/read/23096592/an-effective-system-for-detecting-protein-protein-interaction-based-on-in-vivo-cleavage-by-ppv-nia-protease
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Nuoyan Zheng, Xiahe Huang, Bojiao Yin, Dan Wang, Qi Xie
Detection of protein-protein interaction can provide valuable information for investigating the biological function of proteins. The current methods that applied in protein-protein interaction, such as co-immunoprecipitation and pull down etc., often cause plenty of working time due to the burdensome cloning and purification procedures. Here we established a system that characterization of protein-protein interaction was accomplished by co-expression and simply purification of target proteins from one expression cassette within E...
December 2012: Protein & Cell
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