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https://www.readbyqxmd.com/read/27914088/murine-mycobacterium-marinum-infection-as-a-model-for-tuberculosis
#1
Julia Lienard, Fredric Carlsson
Mycobacteria are a major human health problem globally. Regarding tuberculosis the situation is worsened by the poor efficacy of current vaccine regimens and by emergence of drug-resistant strains (Manjelievskaia J et al, Trans R Soc Trop Med Hyg 110: 110, 2016; Pereira et al., Lancet Infect Dis 12:300-306, 2012; http://www.who.int/tb/publications/global_report/en/) undermining both disease-prevention and available treatments. Thus, increased basic understanding of mycobacterial-and particularly Mycobacterium tuberculosis-virulence strategies and pathogenesis is of great importance...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27913958/the-effect-of-mass-transfer-parameterization-and-ice-retention-on-the-scavenging-and-redistribution-of-so2-by-a-deep-convective-cloud
#2
Vladan Vučković, Dragana Vujović
A chemistry module with the aqueous chemistry coupled with the complex 3D nonhydrostatic atmospheric model is used to investigate how the representation of gas-aqueous mass transfer and ice retention affect the SO2 redistribution in the presence of a convective cloud. Gas uptake to the liquid water is calculated using both Henry's law equilibrium (HE) and kinetic mass transport (KMT). The constant retention coefficients for SO2 (k ret = 0.46) and for H2O2 (k ret = 0.64) are used. It is shown that the amount of SO2 in the air at higher altitudes (10-12 km) is greater when partial retention (PR) is included...
December 2, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27913927/the-number-of-cycles-of-neoadjuvant-chemotherapy-is-associated-with-prognosis-of-stage-iiic-iv-high-grade-serous-ovarian-cancer
#3
Xia Xu, Fei Deng, Mengmeng Lv, Xiaoxiang Chen
OBJECTIVE: No consensus exists on the number of chemotherapy cycles to be administered before and after interval debulking surgery (IDS) in patients with advanced stage epithelial ovarian cancer. The present study aims to explore the optimal number of cycles of neoadjuvant chemotherapy (NAC) and post-operation chemotherapy to treat the International Federation of Gynecology and Obstetrics stage IIIc-IV high-grade serous ovarian cancer (HG-SOC). MATERIALS AND METHODS: A total of 129 IIIc-IV stage HG-SOC cases were retrospectively analyzed...
December 3, 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/27913592/complexin-mutants-reveal-partial-segregation-between-recycling-pathways-that-drive-evoked-and-spontaneous-neurotransmission
#4
Nadezhda Sabeva, Richard W Cho, Alexander Vasin, Agustin Gonzalez, J Troy Littleton, Maria Bykhovskaia
: Synaptic vesicles fuse at morphological specializations in the presynaptic terminal termed active zones (AZs). Vesicle fusion can occur spontaneously or in response to an action potential. Following fusion, vesicles are retrieved and recycled within nerve terminals. It is still unclear whether vesicles that fuse spontaneously or following evoked release share similar recycling mechanisms. Genetic deletion of the SNARE-binding protein complexin dramatically increases spontaneous fusion, with the protein serving as the synaptic vesicle fusion clamp at Drosophila synapses...
December 2, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27913532/t-cell-acute-lymphoblastic-leukemia
#5
Elizabeth A Raetz, David T Teachey
T-cell acute lymphoblastic leukemia (T-ALL) is biologically distinct from its B lymphoblastic (B-ALL) counterpart and shows different kinetic patterns of disease response. Although very similar regimens are used to treat T-ALL and B-ALL, distinctions in response to different elements of therapy have been observed. Similar to B-ALL, the key prognostic determinant in T-ALL is minimal residual disease (MRD) response. Unlike B-ALL, other factors including age, white blood cell count at diagnosis, and genetics of the ALL blasts are not independently prognostic when MRD response is included...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27913319/assessing-the-benefits-of-targeted-drug-delivery-by-nanocarriers-a-partico-pharmacokinetic-framework
#6
Ronald A Siegel, Ameya R Kirtane, Jayanth Panyam
OBJECTIVE: An in vivo kinetic framework is introduced to analyze and predict the quantitative advantage of using nanocarriers to deliver drugs, especially anticancer agents, compared to administering the same drugs in their free form. METHODS: This framework recognizes three levels of kinetics. First is the particokinetics associated with deposition of nanocarriers into tissues associated with drug effect and toxicity, their residence inside those tissues, and elimination of the nanocarriers from the body...
November 29, 2016: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/27913171/effects-of-hydraulic-retention-time-hrt-on-denitrification-using-waste-activated-sludge-thermal-hydrolysis-liquid-and-acidogenic-liquid-as-carbon-sources
#7
Yiding Guo, Liang Guo, Mei Sun, Yangguo Zhao, Mengchun Gao, Zonglian She
Waste activated sludge (WAS) internal carbon source can efficiently and economically enhance denitrification, and hydraulic retention time (HRT) is one of the most important operational parameters for denitrification. The effects of HRT on denitrification were investigated with WAS thermal hydrolysis liquid and acidogenic liquid as carbon sources in this study. The optimal HRT was 12h for thermal hydrolysis liquid and 8h for acidogenic liquid, with NO3(-)-N removal efficiency of 91.0% and 97.6%, respectively...
November 15, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27913147/electro-kinetically-driven-peristaltic-transport-of-viscoelastic-physiological-fluids-through-a-finite-length-capillary-mathematical-modelling
#8
Dharmendra Tripathi, Ashu Yadav, O Anwar Bég
Analytical solutions are developed for the electro-kinetic flow of a viscoelastic biological liquid in a finite length cylindrical capillary geometry under peristaltic waves. The Jefferys' non-Newtonian constitutive model is employed to characterize rheological properties of the fluid. The unsteady conservation equations for mass and momentum with electro-kinetic and Darcian porous medium drag force terms are reduced to a system of steady linearized conservation equations in an axisymmetric coordinate system...
November 29, 2016: Mathematical Biosciences
https://www.readbyqxmd.com/read/27913023/in-stream-metabolism-and-atmospheric-carbon-sequestration-in-a-groundwater-fed-karst-stream
#9
Junbing Pu, Jianhong Li, Mitra B Khadka, Jonathan B Martin, Tao Zhang, Shi Yu, Daoxian Yuan
Atmospheric carbon sequestered in karst systems through dissolution of carbonate minerals is considered to have no net effect on long-term regional and global carbon budgets because precipitation of dissolved carbonate minerals emits CO2 back to the atmosphere. Even though recent studies have implied that rapid kinetics of carbonate dissolution coupled with the aquatic photosynthetic uptake of dissolve inorganic carbon (DIC) could facilitate a stable atmospheric C sink in karst rivers and streams, little is known about the magnitudes and long-term stability of this C sink...
November 29, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27912989/assessment-of-methane-production-from-shredder-waste-in-landfills-the-influence-of-temperature-moisture-and-metals
#10
Ehsan Fathi Aghdam, Charlotte Scheutz, Peter Kjeldsen
In this study, methane (CH4) production rates from shredder waste (SW) were determined by incubation of waste samples over a period of 230days under different operating conditions, and first-order decay kinetic constants (k-values) were calculated. SW and sterilized SW were incubated under different temperatures (20-25°C, 37°C, and 55°C), moisture contents (35% and 75% w/w) and amounts of inoculum (5% and 30% of the samples wet weight). The biochemical methane potential (BMP) from different types of SW (fresh, old and sieved) was determined and compared...
November 29, 2016: Waste Management
https://www.readbyqxmd.com/read/27912968/validation-of-a-thin-layer-chromatography-densitometry-method-for-the-characterization-of-invertase-activity
#11
Justine Ferey, David Da Silva, Sophie Bravo-Veyrat, Pierre Lafite, Richard Daniellou, Benoît Maunit
This paper presents a kinetic study of invertase, a specific fructofuranosidase cloned from the Leishmania major genome. The kinetic parameters of the β-d-fructofuranosidase from Leishmania major (BfrA) were determined using Thin-Layer Chromatography (TLC) and UV-densitometry (TLC@UV) specifically developed for the separation and detection of three carbohydrates namely sucrose, glucose and fructose. Separation was performed on TLC silica gel 60 F254 plates impregnated with sodium bisulphate and citrate and heated prior to development...
November 25, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27912779/ascites-regression-following-neoadjuvant-chemotherapy-in-prediction-of-treatment-outcome-among-stage-iiic-to-iv-high-grade-serous-ovarian-cancer
#12
Xia Xu, Fei Deng, Mengmeng Lv, Binhui Ren, Wenwen Guo, Xiaoxiang Chen
BACKGROUND: No consensus exists on the outcome-related factors of interval debulking surgery (IDS) in patients with advanced high-grade serous ovarian cancer (HG-SOC) who underwent neoadjuvant chemotherapy (NAC). This study aimed to explore the optimal timing for IDS and the prognosis-associated factors of International Federation of Gynecology and Obstetrics stage IIIc to IV HG-SOC patients. METHODS: A total of 160 IIIc to IV stage HG-SOC patients were retrospectively analyzed...
December 2, 2016: Journal of Ovarian Research
https://www.readbyqxmd.com/read/27912317/production-of-bacillus-amyloliquefaciens-og-and-its-metabolites-in-renewable-media-valorisation-for-biodiesel-production-and-p-xylene-decontamination
#13
Augusto Etchegaray, François Coutte, Gabrielle Chataigné, Max Béchet, Ramon H Z Dos Santos, Valérie Leclère, Philippe Jacques
Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after analysis of the gyrA and gyrB DNA sequences. Growth in modified Landy's medium produced 3 main recoverable metabolites: surfactin, fengycin, and acetoin, which promote plant growth...
September 2, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27912193/comparative-tissue-distribution-and-excretion-study-of-alkaloids-from-herba-ephedrae-radix-aconiti-lateralis-extracts-in-rats
#14
Mengyue Ren, Shuai Song, Dedong Liang, Weiting Hou, Xiaomei Tan, Jiabo Luo
Herba Ephedrae-Radix Aconiti Lateralis, composed of Ephedrae (Mahuang in Chinese) and Radix Aconiti Lateralis (Fuzi in Chinese), is a classical herbal combination proven to be effective in treating common cold, asthma, and rheumatoid arthritis. Alkaloids, bioactive components of the herbal extract, have been associated with many side effects. Nine alkaloids, including norephedrine, norpseudoephedrine, ephedrine, pseudoephedrine, methylephedrine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine, were simultaneously quantified within 14...
November 13, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27912190/nanostructured-3d-porous-graphene-hydrogel-based-ti-sb-sno2-gr-electrode-with-enhanced-electrocatalytic-activity
#15
Sumreen Asim, Yunqing Zhu, Masud Rana, Jiao Yin, Muhammad Wajid Shah, Yingxuan Li, Chuanyi Wang
Nanostructured highly porous 3D-Ti/Sb-SnO2-Gr electrode, based on 3D porous graphene hydrogel was fabricated via a fast-evaporation technique through layer by layer (LBL) deposition. The 3D pores are uniformly distributed on the high fidelity of substrate with pore sizes of 7-12 nm, as confirmed by SEM analysis. Compared to Ti/Sb-SnO2 electrode, the fabricated 3D porous electrode possesses high oxygen evolution potential (2.40 V), smaller charge transfer resistance (29.40 Ω cm(-2)), higher porosity (0...
November 29, 2016: Chemosphere
https://www.readbyqxmd.com/read/27912185/sorption-of-vanadium-v-onto-natural-soil-colloids-under-various-solution-ph-and-ionic-strength-conditions
#16
Xiuhua Luo, Lin Yu, Changzhao Wang, Xianqiang Yin, Ahmed Mosa, Jialong Lv, Huimin Sun
Batch sorption kinetics and isothermal characteristics of V(V) were investigated on three natural soil colloids (manual loessial soil colloid (MSC), aeolian sandy soil colloid (ASC), and cultivated loessial soil colloid (CSC)) under various solution pH and ionic strength (IS) conditions. Colloids were characterized by atomic force microscopy (AFM), X-ray diffraction (XRD), and fourier transform infrared spectroscopy (FTIR). AFM micrographs showed CSC with an aggregated shape with larger particle diameter as compared with ASC and MSC...
November 29, 2016: Chemosphere
https://www.readbyqxmd.com/read/27912158/direct-detection-of-phenylephrine-3-o-sulfate-in-ls180-human-intestinal-cells-using-a-novel-hydrophilic-interaction-liquid-chromatography-hilic-assay
#17
Heta N Shah, Matthew T Halquist, Phillip M Gerk
The efficacy of phenylephrine (PE) is controversial due to its extensive pre-systemic metabolism through sulfation to form phenylephrine-3-O-sulfate (PES). Hence quantitation of PES is important in order to study the metabolism of PE. There are no published methods available for direction detection of PES. We have developed and validated a hydrophilic interaction liquid chromatography (HILIC) method for the direct detection of PES and simultaneous detection of PE to study the enzyme kinetics and metabolism of PE to enable approaches to reduce the presystemic metabolism of PE...
November 13, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27912137/a-novel-continuous-two-phase-partitioning-bioreactor-operated-with-polymeric-tubing-performance-validation-for-enhanced-biological-removal-of-toxic-substrates
#18
M Concetta Tomei, Domenica Mosca Angelucci, Andrew J Daugulis
A continuous two-phase partitioning bioreactor (C-TPPB), operated with coiled tubing made of the DuPont polymer Hytrel 8206, was tested for the bioremediation of 4-chlorophenol, as a model toxic compound. The tubing was immersed in the aqueous phase, with the contaminated water flowing tube-side, and an adapted microbial culture suspended in the bioreactor itself, with the metabolic demand of the cells creating a concentration gradient to cause the substrate to diffuse into the bioreactor for biodegradation...
November 29, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27912125/feooh-graphene-oxide-nanocomposites-for-fluoride-removal-from-water-acetate-mediated-nano-feooh-growth-and-adsorption-mechanism
#19
Liyuan Kuang, Yuyang Liu, Dandan Fu, Yaping Zhao
Fluoride adsorption capacity in water matrices depends highly on the properties of each component of the adsorption system, the most important one of these is the physchemical properties of the adsorbent. Nanoparticle Goethite anchored onto graphene oxide (FeOOH+Ac/GO) and rice spike-like Akaganeite anchored onto graphene oxide (FeOOH/GO) were synthesized via an in-situ hydrolysis procedure and compared their fluoride adsorption performances in order to address the effect of crystalline structure growth induced by acetate sodium (NaAc), one important organic ligand in water and soil...
November 21, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27912123/synthesis-of-hierarchical-porous-zinc-oxide-zno-microspheres-with-highly-efficient-adsorption-of-congo-red
#20
Chunsheng Lei, Meng Pi, Chuanjia Jiang, Bei Cheng, Jiaguo Yu
Hierarchical porous zinc oxide (ZnO) was successfully synthesized via a facile hydrothermal method followed by calcination, and characterized by X-ray diffraction, field-emission scanning electron microscopy, transmission electron microscopy, nitrogen adsorption-desorption, and Fourier transform infrared spectroscopy analyses. The as-prepared porous ZnO exhibits microsphere morphologies with diameters of 6-8μm, which are assembled from two-dimensional nanosheets. The as-prepared hierarchical porous ZnO microspheres possessed high specific surface areas (57m(2)/g), and were evaluated for adsorption of Congo red (CR) in aqueous solution...
November 15, 2016: Journal of Colloid and Interface Science
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