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osteonecrosis gene

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https://www.readbyqxmd.com/read/29760725/the-effects-of-hyperacute-serum-on-the-elements-of-the-human-subchondral-bone-marrow-niche
#1
Melinda Simon, Bálint Major, Gabriella Vácz, Olga Kuten, István Hornyák, Adél Hinsenkamp, Dorottya Kardos, Marcell Bagó, Domonkos Cseh, Adrienn Sárközi, Denes Horvathy, Stefan Nehrer, Zsombor Lacza
Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type. Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as "proliferative agents" for the topical MSC pool...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29758548/stromal-cell-derived-factor-sdf-1-alpha-overexpression-promotes-bone-regeneration-by-osteogenesis-and-angiogenesis-in-osteonecrosis-of-the-femoral-head
#2
Fan Yang, Feng Xue, Junjie Guan, Zeng Zhang, Jimin Yin, Qingling Kang
BACKGROUND/AIMS: Osteonecrosis of the femoral head (ONFH) is a devastating orthopedic disease. Previous studies suggested that stromal-cell-derived factor (SDF)-1 was involved in osteogenesis and angiogenesis. However, whether SDF-1 potentiates the angiogenesis and osteogenesis of bone marrow-derived stromal stem cells (BMSCs) in ONFH is not clear. METHODS: BMSCs were transfected with green fluorescent protein (GFP) or the fusion gene encoding GFP and SDF-1α, and transgenic efficacy was monitored by immunofluorescence...
May 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29700584/microrna-mediated-interacting-circuits-predict-hypoxia-and-inhibited-osteogenesis-of-stem-cells-and-dysregulated-angiogenesis-are-involved-in-osteonecrosis-of-the-femoral-head
#3
Gour-Shenq Kao, Yuan-Kun Tu, Pei-Hsun Sung, Feng-Sheng Wang, Yu-Der Lu, Chen-Ta Wu, Rio L C Lin, Hon-Kan Yip, Mel S Lee
PURPOSE: MicroRNAs (miRNAs) are associated with various pathologic conditions and can serve as diagnostic or therapeutic biomarkers. This study tried to identify the differentially expressed miRNAs to predict the possible pathomechanisms involved in osteonecrosis of the femoral head (ONFH). METHODS: We compared the peripheral blood miRNAs in 46 patients with ONFH and 85 healthy controls by microarray and droplet digital polymerase chain reaction (ddPCR). Putative interacted networks between the differentially responded miRNAs were analyzed by web-based bioinformatics prediction tools...
April 26, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29650420/denosumab-treatment-of-inoperable-or-locally-advanced-giant-cell-tumor-of-bone-multicenter-analysis-outside-clinical-trial
#4
Piotr Rutkowski, Louie Gaston, Aneta Borkowska, Silvia Stacchiotti, Hans Gelderblom, Giacomo Giulio Baldi, Emanuela Palmerini, Paolo Casali, Alessandro Gronchi, Michael Parry, Domenico Andrea Campanacci, Guido Scoccianti, Michal Wagrodzki, Stefano Ferrari, Sander Dijkstra, Andrzej Pieńkowski, Robert Grimer
BACKGROUND: Giant cell tumor of bone (GCTB) is an osteolytic, locally aggressive, rarely metastazing bone tumor. This is a retrospective study evaluating a large series of GCTB patients treated with denosumab in routine practice in 6 European reference centers. METHODS: Patients with locally advanced, unresectable or metastatic GCTB, treated with denosumab outside clinical trials were eligible. Primary end-point was progression-free survival (PFS) for all patients; secondary end-points were: type of surgery, relapse rate and event-free survival for patients after preoperative denosumab + surgery...
March 31, 2018: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29590087/microrna-expression-profiling-of-bone-marrow-mesenchymal-stem-cells-in-steroid-induced-osteonecrosis-of-the-femoral-head-associated-with-osteogenesis
#5
Ao Wang, Ming Ren, Yang Song, Xiaonan Wang, Qingyu Wang, Qiwei Yang, He Liu, Zhenwu Du, Guizhen Zhang, Jincheng Wang
BACKGROUND Steroid-induced osteonecrosis of the femoral head (SONFH) is a common orthopedic disease associated with the application of glucocorticoid (GC). In this study, we detected the microRNAs (miRNAs) differentially expressed in bone marrow mesenchymal stem cells (BMSCs) from SONFH patients, and target gene predictions were performed, and the functions of the target genes was verified. MATERIAL AND METHODS BMSCs collected from patients with SONFH and femoral neck fracture (FNF) constituted the SONFH group (n=3) and FNF (control) group (n=3), respectively...
March 28, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29546453/altered-microrna-expression-profile-in-the-peripheral-lymphoid-compartment-of-multiple-myeloma-patients-with-bisphosphonate-induced-osteonecrosis-of-the-jaw
#6
Caterina Musolino, Giacomo Oteri, Alessandro Allegra, Manuela Mania, Angela D'Ascola, Angela Avenoso, Vanessa Innao, Andrea Gaetano Allegra, Salvatore Campo
Bisphosphonates are formidable inhibitors of osteoclast-mediated bone resorption employed for therapy of multiple myeloma (MM) subjects with osteolytic lesions. Osteonecrosis of the jaw (ONJ) is an uncommon drug-induced adverse event of these agents. MicroRNAs (miRNAs) are a group of small, noncoding RNAs nucleotides, which are essential post-transcriptional controllers of gene expression. They have a central role in the normal bone development. The goal of our study was to investigate 18 miRNAs, whose targets were previously validated and described in MM subjects without ONJ, in peripheral lymphocytes of MM subjects with bisphosphonate-induced ONJ...
March 15, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29511371/bisphosphonate-modulation-of-the-gene-expression-of-different-markers-involved-in-osteoblast-physiology-possible-implications-in-bisphosphonate-related-osteonecrosis-of-the-jaw
#7
Francisco Javier Manzano-Moreno, Javier Ramos-Torrecillas, Lucia Melguizo-Rodríguez, Rebeca Illescas-Montes, Concepción Ruiz, Olga García-Martínez
The aim of the present study was to elucidate the role of osteoblasts in bisphosphonates-related osteonecrosis of the jaw (BRONJ). The specific objective was to evaluate the effect on osteoblasts of two nitrogen-containing BPs (zoledronate and alendronate) and one non-nitrogen-containing BP (clodronate) by analyzing modulations in their expression of genes essential for osteoblast physiology. Real-time polymerase chain reaction (RT-PCR) was used to study the effects of zoledronate, alendronate, and clodronate at doses of 10-5 , 10-7 , or 10-9 M on the expression of Runx-2, OSX, ALP, OSC, OPG, RANKL, Col-I, BMP-2, BMP-7, TGF-β1, VEGF, TGF-βR1, TGF-βR2, and TGF-βR3 by primary human osteoblasts (HOBs) and MG-63 osteosarcoma cells...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29408208/effect-of-glucocorticoids-on-mirna-expression-spectrum-of-rat-femoral-head-microcirculation-endothelial-cells
#8
Ju'an Yue, Fuyin Wan, Qidong Zhang, Pengfei Wen, Liming Cheng, Ping Li, Wanshou Guo
The study profiled the differential miRNA expression from femoral head bone microvascular endothelial cells (BMECs) between model group and control group to explore the pathogenesis of steroid-induced osteonecrosis of femoral head (ONFH). Twenty 8-week-old Female Sprague-Dawley (SD) rats were randomly divided into control and model groups. Rats in model group received an intraperitoneal injection of 20-μg/kg lipopolysaccharide (LPS) at an interval of 24 h. Then, 24 h later, rats received three doses of 40-mg/kg methylprednisolone by intramuscular injection at intervals of 24 h...
April 20, 2018: Gene
https://www.readbyqxmd.com/read/29382571/fgf2-and-fam201a-affect-the-development-of-osteonecrosis-of-the-femoral-head-after-femoral-neck-fracture
#9
Gangyong Huang, Guanglei Zhao, Jun Xia, Yibing Wei, Feiyan Chen, Jie Chen, Jingsheng Shi
Osteonecrosis of the femoral head (ONFH) is a common orthopedic disease associated with high disability, and femoral neck fracture (FNF) is one of the most common reasons for traumatic ONFH. This study was designed to reveal the mechanisms underlying ONFH. Using fastx_toolkit and prinseq-lite tools, quality control was conducted for the sequencing data. The differentially expressed genes (DEGs, including both mRNAs and lncRNAs) between ONFH and FNF samples were identified using the edgeR package in R, and were then subjected to enrichment analysis using the BioCloud platform...
April 30, 2018: Gene
https://www.readbyqxmd.com/read/29374435/a-human-chondrocyte-derived-in-vitro-model-of-alcohol-induced-and-steroid-induced-femoral-head-necrosis
#10
Xiong Qin, Pan Jin, Tongmeng Jiang, Muyan Li, Jiachang Tan, Huayu Wu, Li Zheng, Jinmin Zhao
BACKGROUND Worldwide, femoral head necrosis (FHN), which is also known as avascular necrosis of the femoral head or osteonecrosis of the femoral head, affects millions of people. Excess alcohol intake and steroid use are two common associations with FHN, but their pathogenesis remains unknown. The aim of this study was to develop an in vitro model using human chondrocytes to study alcohol-induced and steroid-induced FHN. MATERIAL AND METHODS In this study, the in vitro model used a monolayer culture of articular chondrocytes derived from patients with non-traumatic FHN (Ficat and Arlet, Stage III)...
January 27, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29312574/association-between-mmp-3-polymorphisms-among-chinese-patients-with-osteonecrosis-of-the-femoral-head
#11
Yuxin Qi, Yong Zhu, Yuju Cao, Huiqiang Wu, Mingqi Sun, Hao Wu, Linlin Pan, Guoqiang Wang, Jianzhong Wang
Many potential causative factors are related to the initiation and progression of osteonecrosis of the femoral head (ONFH). The matrix metalloproteinase/tissue inhibitor of metalloproteinases (MMPs/TIMPs) system was found to play a significant role in the development of ONFH. The aim of this study is to investigate the association between polymorphisms of MMP-3 and ONFH in the Chinese population. We selected 8 single-nucleotide polymorphisms (SNPs) in 2 genes selected from the MMPs/TIMPs system in a case-control study with 585 cases of ONFH and 507 healthy controls...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29286579/systematic-review-of-pharmacogenomics-and-adverse-drug-reactions-in-paediatric-oncology-patients
#12
REVIEW
Rachel Conyers, Subalatha Devaraja, David Elliott
Many paediatric patients with cancer experience significant chemotherapy side effects. Predisposition to drug reactions is governed by single nucleotide polymorphisms (SNPs). We performed a systematic review of the literature from 2006 through 2016. Outcomes of interest included patient characteristics, cancer type drug of interest, genes investigated, toxicity identified and genetic polymorphisms implicated. The primary toxicities studied were neurotoxicity cardiotoxicity, osteonecrosis, and thromboembolism and hypersensitivity reactions...
April 2018: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/29282361/influence-of-bcl2l11-polymorphism-on-osteonecrosis-during-treatment-of-childhood-acute-lymphoblastic-leukemia
#13
Maria Plesa, Vincent Gagné, Sanja Glisovic, Melissa Younan, Bahram Sharif-Askari, Caroline Laverdière, Nathalie Alos, Jean-Marie Leclerc, Stephen E Sallan, Donna Neuberg, Jeffery L Kutok, Lewis B Silverman, Daniel Sinnett, Maja Krajinovic
Osteonecrosis (ON) is corticosteroid-related complication, reported in children with acute lymphoblastic leukemia (ALL). We have previously found that polymorphisms in BCL2L11 gene coding for pro-apoptotic Bim protein influence reduction of overall survival (OS) in a corticosteroid (CS) dose-dependent manner in childhood ALL patients. The same set of SNPs was here investigated for an association with CS-related ON assessed retrospectively in 304 children with ALL from Quebec (QcALL cohort) who received Dana-Farber Cancer Institute (DFCI) ALL treatment protocols...
December 27, 2017: Pharmacogenomics Journal
https://www.readbyqxmd.com/read/29205600/circulating-microrna-signature-of-steroid-induced-osteonecrosis-of-the-femoral-head
#14
Zheng Li, Chao Jiang, Xingye Li, William K K Wu, Xi Chen, Shibai Zhu, Chanhua Ye, Matthew T V Chan, Wenwei Qian
OBJECTIVES: Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopaedic disease of which early detection remains clinically challenging. Accumulating evidences indicated that circulating microRNAs (miRNAs) plays vital roles in the development of several bone diseases. However, the association between circulating miRNAs and steroid-induced ONFH remains elusive. MATERIALS AND METHODS: miRNA microarray was performed to identify the differentially abundant miRNAs in the serums of systemic lupus erythematosus (SLE) patients with steroid-induced ONFH as compared with SLE control and healthy control group...
February 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29188452/bisphosphonates-hinder-osteoblastic-osteoclastic-differentiation-in-the-maxillary-sinus-mucosa-derived-stem-cells
#15
Jun Zhang, Jaesuh Park, Jung-Woo Lee, Yong-Dae Kwon, Eun-Cheol Kim
OBJECTIVES: Although bisphosphonates (BPs) are known to be associated with osteonecrosis of the maxilla, the precise effects of BPs on bone metabolism in human maxillary sinus mucosal cells (HMSMCs) are not yet known. The purposes of this study were to examine the effects of the BPs zoledronate (ZOL) and alendronate (ALN) on osteoblastic and osteoclastic differentiation in HMSMCs and to investigate the signaling pathways involved. MATERIALS AND METHODS: The effects of ZOL and ALN were assessed for osteoblast differentiation by alkaline phosphatase (ALP) activity, alizarin red staining, and RT-PCR for genes encoding Runx2 and osterix...
November 30, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29131454/emerging-roles-of-micrornas-in-osteonecrosis-of-the-femoral-head
#16
REVIEW
Zheng Li, Bo Yang, Xisheng Weng, Gary Tse, Matthew T V Chan, William Ka Kei Wu
Osteonecrosis of the femoral head (ONFH) is one of the most common orthopaedic diseases. The exact pathogenic mechanism of ONFH is still unknown. MicroRNAs (miRNAs) are a class of non-coding RNAs that negatively modulate gene expression at post-transcriptional level. An increasing number of studies have shown that miRNAs play crucial roles in different physiological processes, including development, cell proliferation, differentiation and metabolism. Recently, multiple studies demonstrated that miRNAs are involved in the pathogenesis of ONFH...
February 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29120670/correlation-between-steroid-induced-osteonecrosis-of-the-femoral-head-and-hepatic-cyp3a-activity-a-systematic-review-and-meta-analysis
#17
Fa-Qi Guo, Min Deng
BACKGROUND: Steroid-induced osteonecrosis of the femoral head (ONFH) in young adults is a challenging disorder that can impairs the quality of life of a patient. The disease also leads to frequent occurrences of collapse of the femoral head and resultant dysfunction of the hip joint. In recent years, some scholars have studied steroid-induced lipid metabolism disorder and achieved the effect of steroid-induced ONFH treatment. This study aims to review the investigations on the hepatic CYP3A (cytochrome P4503A enzyme) genetic polymorphisms in steroid-induced ONFH patients...
November 9, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/29118346/genome-wide-association-study-of-idiopathic-osteonecrosis-of-the-femoral-head
#18
Yuma Sakamoto, Takuaki Yamamoto, Nobuhiko Sugano, Daisuke Takahashi, Toshiyuki Watanabe, Takashi Atsumi, Junichi Nakamura, Yukiharu Hasegawa, Koichi Akashi, Ichiei Narita, Takeshi Miyamoto, Tsutomu Takeuchi, Katsunori Ikari, Koichi Amano, Atsuhiro Fujie, Toshikazu Kubo, Yoshifumi Tada, Ayumi Kaneuji, Hiroaki Nakamura, Tomoya Miyamura, Tamon Kabata, Ken Yamaji, Takahiro Okawa, Akihiro Sudo, Kenji Ohzono, Yoshiya Tanaka, Yuji Yasunaga, Shuichi Matsuda, Yuuki Imai, Masato Akiyama, Michiaki Kubo, Yoichiro Kamatani, Yukihide Iwamoto, Shiro Ikegawa
Idiopathic osteonecrosis of the femoral head (IONFH) is an ischemic disorder that causes bone necrosis of the femoral head, resulting in hip joint dysfunction. IONFH is a polygenic disease and steroid and alcohol have already known to increase its risk; however, the mechanism of IONFH remains to be elucidated. We performed a genome-wide association study using ~60,000 subjects and found two novel loci on chromosome 20q12 and 12q24. Big data analyses identified LINC01370 as a candidate susceptibility gene in the 20q12 locus...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29090520/an-investigation-of-toxicities-and-survival-in-hispanic-children-and-adolescents-with-all-results-from-the-dana-farber-cancer-institute-all-consortium-protocol-05-001
#19
Justine M Kahn, Peter D Cole, Traci M Blonquist, Kristen Stevenson, Zhezhen Jin, Sergio Barrera, Randy Davila, Emily Roberts, Donna S Neuberg, Uma H Athale, Luis A Clavell, Caroline Laverdiere, Jean-Marie Leclerc, Bruno Michon, Marshall A Schorin, Jennifer J G Welch, Stephen E Sallan, Lewis B Silverman, Kara M Kelly
PURPOSE: This study compared the relative incidence of treatment-related toxicities and the event-free and overall survival between Hispanic and non-Hispanic children undergoing therapy for acute lymphoblastic leukemia (ALL) on Dana-Farber Cancer Institute ALL Consortium protocol 05-001. PATIENTS AND METHODS: Secondary analysis of prospectively collected data from a phase III multicenter study in children and adolescents of 1-18 years with previously untreated ALL...
March 2018: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/29069795/variants-of-gsk3%C3%AE-and-sfrp4-genes-in-wnt-signaling-were-not-associated-with-osteonecrosis-of-the-femoral-head
#20
Yang Song, Zhenwu Du, Qiwei Yang, Ming Ren, Qingyu Wang, Gaoyang Chen, Haiyue Zhao, Zhaoyan Li, Guizhen Zhang
Genome-wide association studies have identified that the gene variants in Wnt signaling associate with bone mineral density and fracture risk but the effects of the variants on the development of osteonecrosis of the femoral head (ONFH) have been unclear. Here, we analyzed the polymorphisms of 4 variants in GSK3β and SFRP4 genes of Wnt signaling and their association with the development of ONFH through Mass ARRAY® platform in 200 ONFH patients and 177controls in Chinese population. Our results showed that the genotypes and allele frequencies of all variants detected in SFRP4 and GSK3β genes were not significantly different between patients and controls ( p > 0...
September 22, 2017: Oncotarget
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