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Syphilis IgG

Carmenza Macía, Sandra Vargas, Ana María Mora, Ashly Melissa Sarmiento, Robinson Pacheco, Fernando Rosso
INTRODUCTION: Human lymphotropic virus (HTLV I/II) is a retrovirus that is prevalent across the Colombian Pacific coast, and is potentially transmissible by transfusion. Blood bank screening has been regulated since 2004, in order to reduce transmission of HTLV I/II through donation. Information on the seroprevalence of the virus in southwestern Colombia is limited.  OBJECTIVE: To determine the seroprevalence and the behavior of reactivity to HTLV I/II before and after the introduction of Western blot, and the comorbidity of HTLV and other infectious markers in donors from a blood bank in Cali, Colombia...
2016: Biomédica: Revista del Instituto Nacional de Salud
Francesco Drago, Giulia Ciccarese, Carlo F Tomasini, Paola Calamaro, Maurizio Boggio, Alfredo Rebora, Aurora Parodi
We describe herein a woman who developed subcutaneous gummas in her trochanteric regions, bilaterally, although she had been treated for syphilis two decades earlier. Evidence of Treponema pallidum latent late infection was the presence of IgG antibodies against T. pallidum and the positive non-treponemal and treponemal tests. Moreover, immunohistochemical staining for T. pallidum detected some spirochetes close to the atrophic adipocytes allowing the diagnosis of lypo-atrophic panniculitis tertiary syphilis...
August 24, 2016: International Journal of STD & AIDS
Haiyang Hu, Xiaoyan Liu, Zhi Zhang, Xiaoqin Xu, Lingen Shi, Gengfeng Fu, Xiping Huan, Ying Zhou
Epidemics of HIV among men who have sex with men (MSM) are major public health concerns in most parts of China. This study examined the trends in HIV incidence and associated factors among MSM in Jiangsu Province. Five consecutive cross-sectional surveys were conducted among MSM from 2011 to 2015 in eight cities throughout Jiangsu Province. Participants were recruited from MSM venues or via the internet. Demographic and behavioral data were collected through HIV bio-behavioral surveys. Blood specimens were collected to test for HIV and syphilis...
2016: International Journal of Environmental Research and Public Health
Daniel R Zweitzig, Arjan G Tibbe, Ai T Nguyen, Cees J M van Rijn, Mark J Kopnitsky, Kathleen Cichonski, Leon W M M Terstappen
The intrinsic properties of silicon microsieves, such as an optically flat surface, high overall porosity, and low flow resistance have led to an increasing number of biotechnology applications. In this report, the feasibility of creating a microsieve-based immunoassay platform was explored. Microsieves containing 5μm pores were coupled with poly-acrylic acid chains, and then mounted into a plastic holder to enable rapid reagent exchanges via a wicking mechanism. The mounted microsieves were coated with infectious disease-related antigens at [2...
October 2016: Journal of Immunological Methods
Wei Liu, Hui Xin Liu, Lin Zhang, Xue Xia Hou, Kang Lin Wan, Qin Hao
OBJECTIVE: In this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB. METHODS: Six recombinant antigens, Fla B.g, OspC B.a, OspC B.g, P39 B.g, P83 B.g, and VlsE B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic (ROC) curves, and the sensitivity and specificity of each protein was evaluated...
May 2016: Biomedical and Environmental Sciences: BES
Qixia Chen, Jingna An, Chenli Rao, Tingting Wang, Dongdong Li, Shu Feng, Chuanmin Tao
BACKGROUND: Syphilis is a major concern to global public health with increasing incidence. So its screening test should have sufficient sensitivity and specificity. We evaluated the performance of the Lumipulse G TP-N assay detection for syphilis screening and compared it with the InTec ELISA test kit for TP, which is widely used. METHODS: Samples of several patient groups including 133 clinical and serologically characterized syphilitic sera, 175 samples containing potentially interfering agents, and 2290 unselected samples submitted for routine screening were detected by both the Lumipulse G TP-N assay and the InTec ELISA test kit for TP...
2016: Clinical Laboratory
Zhong Tang, Zhisheng Liang, Yi Nong, Xiaochun Wu, Hui Luo, Kun Gao
The purpose of this study was to apply goldmag immunoprobes into establishment of nanoparticles-based colorimetric assay as well as construction of immunochromatography quantitative and qualitative system by exploring point-of-care testing of syphilis with goldmag particles carrier-based immunoprobe and analysis of spatial data of Geographic Information System (GIS) platform. Goat anti-rabbit immunoglobulin G (IgG) was coupled on the surface of modified nanoparticles, taking N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide as the connector...
May 6, 2016: Artificial Cells, Nanomedicine, and Biotechnology
Tao-Jun He, Fan Mo, Xiao-You Xiao, Qiao-Yun Dan, Su-Jie Li, Yin-Hui Zhang, Xue-Dong Lu
BACKGROUND: The false positive in conventional syphilis serological test was found in patients with multiple myeloma (MM). OBJECTIVE: To investigate the relationship between the M-protein of patients with MM and the false positive in conventional syphilis serologic test. METHODS: The M-protein of 68 MM cases was typed with immunofixation electrophoresis and 68 cases of MM were screened with non-specific and specific syphilis serologic tests, then the samples with syphilic serological positive were chosen and confirmed with immonobloting test, finally the relationship between M protein of MM and the false positive of syphilis serological test were analysed...
April 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ronald Salamano, Raquel Ballesté, Abayubá Perna, Natalia Rodriguez, Diego Lombardo, Natalia García, Pablo López, Pablo Cappuccio
Lumbar puncture in neurologically asymptomatic HIV+ patients is still under debate. There are different criteria for detecting neurosyphilis through cerebrospinal fluid (CSF), especially in cases that are negative through the Venereal Disease Research Laboratory (VDRL), regarding cellularity and protein content. However, a diagnosis of neurosyphilis can still exist despite negative VDRL. Treponema pallidum hemagglutination assay (TPHA) titers and application of the TPHA index in albumin and IgG improve the sensitivity, with a high degree of specificity...
February 2016: Arquivos de Neuro-psiquiatria
Antonella Marangoni, Claudio Foschi, Maria Grazia Capretti, Paola Nardini, Monica Compri, Luigi Tommaso Corvaglia, Giacomo Faldella, Roberto Cevenini
Serology has a pivotal role in the diagnosis of congenital syphilis (CS), but problems arise because of the passive transfer of IgG antibodies across the placenta.The aim of this study was to assess the diagnostic value of a comparative Western Blot (WB) method finalized to match IgG immunological profiles of mothers and their own babies at birth, in order to differentiate between passively transmitted maternal antibodies and antibodies synthesized by the infants against Treponema pallidum.Thirty infants born to mothers with unknown or inadequate treatment for syphilis entered in a retrospective study, conducted at St...
March 9, 2016: Clinical and Vaccine Immunology: CVI
Y Xie, M Xu, C Wang, J Xiao, Y Xiao, C Jiang, X You, F Zhao, T Zeng, S Liu, X Kuang, Y Wu
UNLABELLED: Syphilis is a multistage sexually transmitted disease that remains a serious public health concern worldwide. The coexistence of Treponema pallidum with other closely related members of spirochaeta, such as Leptospira spp. and Borrelia burgdorferi, has complicated the serodiagnosis due to cross-reactive antigens. In this study, recombinant Tp0821 protein was expressed in Escherichia coli and purified by metal affinity chromatography. Then enzyme-linked immunosorbent assays (ELISAs) based on Tp0821 for the detection of specific antibodies were established...
April 2016: Letters in Applied Microbiology
Sheikh M Talha, Petri Saviranta, Liisa Hattara, Tytti Vuorinen, Jukka Hytönen, Navin Khanna, Kim Pettersson
Multiplex assays detecting sets of related clinical analytes simultaneously can save considerable amount of time and resources. Array-in-well (AIW) is a powerful platform for the multiplex detection of different analytes where microarrays can be printed at the bottom of microtiter wells, thus combining the potential of microarrays with the ease of handling microtiter wells. We have developed a single-step AIW assay for the simultaneous screening of HIV, Treponema pallidum subspecies pallidum (causing syphilis) and Hepatitis B virus infections targeting the specific detection of anti-HIV- and treponemal-antibodies and Hepatitis B surface antigen (HBsAg), respectively, using two different fluorescent label technologies i...
February 2016: Journal of Immunological Methods
Chuanhao Jiang, Jinhong Xiao, Yafeng Xie, Yongjian Xiao, Chuan Wang, Xingxing Kuang, Man Xu, Ranhui Li, Tiebing Zeng, Shuanquan Liu, Jian Yu, Feijun Zhao, Yimou Wu
Syphilis is a multistage disease caused by the invasive spirochete Treponema pallidum subsp. pallidum, and accurate diagnosis is important for the prevention and treatment of syphilis. Here, to identify appropriate diagnostic antigens for serodiagnosis of syphilis, 6 recombinant proteins were expressed in Escherichia coli and purified, including flagellins (FlaB1 [Tp0868], FlaB2 [Tp0792], and FlaB3 [Tp0870]), Tp0463, Tp0751, and Tp1038. The sensitivities were determined by screening sera from individuals with primary (n=82), secondary (n=115), latent (n=105), and congenital (n=65) syphilis...
February 2016: Diagnostic Microbiology and Infectious Disease
John D Kriesel, Amiteshwar S Bhatia, Cammie Barrus, Mike Vaughn, Jordan Gardner, Robert J Crisp
Current sexually transmitted disease testing is not optimal due to delays in reporting or missed diagnoses due to a lack of comprehensive testing. The FilmArray® (BioFire Diagnostics, LLC, Salt Lake City, Utah) is a user-friendly, fully automated, multiplex PCR system that is being developed for rapid point-of-care use. A research-use-only sexually transmitted disease panel including multiple PCR primer sets for each organism was designed to detect Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, Ureaplasma urealyticum, Haemophilus ducreyi, and herpes simplex virus types 1 and 2...
November 3, 2015: International Journal of STD & AIDS
Atsushi Ito, Noriyasu Niizeki, Hitomi Kurose, Takatoshi Yonezawa, Rie Sasaki, Mineji Tachibana, Yutaka Tomoda, Shuichi Kino, Satoshi Fujii
Serological diagnosis of syphilis can be made by using the serological test for syphilis (STS) method for detecting a lipid antibody and Treponema pallidum (TP) method for detecting the anti-TP-specific antibody. In STS and TP methods, the basis using latex agglutination reaction has been used in many facilities. However, in latex agglutination, false-positive results due to non-specific reaction have sometimes been obtained in reactions of a routine laboratory test reagent detecting the anti-TP antibody used in our medical laboratory...
January 2015: Rinsho Byori. the Japanese Journal of Clinical Pathology
John J Halperin
Lyme borreliosis, infection with the tick-borne spirochete Borrelia burgdorferi sensu lato, causes nervous system involvement in 10-15 % of identified infected individuals. Not unlike the other well-known spirochetosis, syphilis, infection can be protracted, but is microbiologically curable in virtually all patients, regardless of disease duration. Diagnosis relies on 2-tier serologic testing, which after the first 4-6 weeks of infection is both highly sensitive and specific. After this early, acute phase, serologic testing should rely only on IgG reactivity...
March 2016: Acta Neurologica Belgica
Emine Ünal, Müzeyyen Gönül, Seray Çakmak, Ayşegül Yalçınkaya Iyidal, Arzu Kılıç, Ülker Gül, Pinar Doner
INTRODUCTION: Human papillomavirus (HPV) is one of the most common causes of sexually transmitted diseases (STD). The incidence of condyloma acuminata (CA) has increased in recent years. AIM: To determine demographical features and serological test results of STD in patients with CA. MATERIAL AND METHODS: A cross-sectional survey was conducted on 94 patients presenting to a dermatology clinic in Ankara, Middle Anatolia, Turkey. Dermatological examinations were made and the patients completed a questionnaire which consisted of questions about their marital status, partners and condom use...
August 2015: Postȩpy Dermatologii i Alergologii
Na-Li Huang, Lei Ye, Marion E Schneider, Yi-Xin Du, Yuan-Hong Xu, Li-Bin Fan, Wei-Dong Du
In this study, we developed a novel protein biochip methodology that was characterized by dithiobis (succinimidyl undecanoate) (DSU) and specialized for detection of serum IgG and IgM antibodies against Treponema pallidum pathogens in the patients with syphilis, respectively. The biochips were validated by a dimension of atomic force microscope (AFM). The visualized detection limit of IgG antibody on the biochip was 0.39μg/ml. Finally, 286 serum samples from the patients with syphilis were simultaneously tested on the rTpN15-17-47 coated biochips...
January 15, 2016: Biosensors & Bioelectronics
S Jonckheere, M Berth, M Van Esbroeck, S Blomme, K Lagrou, E Padalko
The Architect Syphilis TP is considered to be a suitable screening test due to its high sensitivity and full automation. According to the International Union against Sexually Transmitted Infections (IUSTI) 2014 guidelines, however, positive screening tests need confirmation with Treponema pallidum particle agglutination (TP.PA). Among Architect-positive results, samples with a negative non-treponemal test present the major diagnostic challenge. In this multicenter study, we investigated if other, preferable less labor-intensive treponemal tests could replace TP...
October 2015: European Journal of Clinical Microbiology & Infectious Diseases
Melita Vukšić Polić, Vera Plužarić, Igor Kuric
No abstract text is available yet for this article.
2015: Acta Dermatovenerologica Croatica: ADC
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