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Io Antonopoulou, Laura Leonov, Peter Jütten, Gabriella Cerullo, Vincenza Faraco, Adamantia Papadopoulou, Dimitris Kletsas, Marianna Ralli, Ulrika Rova, Paul Christakopoulos
Five feruloyl esterases (FAEs; EC, FaeA1, FaeA2, FaeB1, and FaeB2 from Myceliophthora thermophila C1 and MtFae1a from M. thermophila ATCC 42464, were tested for their ability to catalyze the transesterification of vinyl ferulate (VFA) with prenol in detergentless microemulsions. Reaction conditions were optimized investigating parameters such as the medium composition, the substrate concentration, the enzyme load, the pH, the temperature, and agitation. FaeB2 offered the highest transesterification yield (71...
January 11, 2017: Applied Microbiology and Biotechnology
Feray Koc
PURPOSE: To report the causes of persistent inferior oblique (IO) overactions after disinsertion procedure. METHODS: Surgical findings of nine eyes of eight patients who needed secondary surgery to the IO muscles because of persistent overaction after IO disinsertion were evaluated retrospectively. Inferior obliques were found partially retracted into their sheath, and some parts of the proximal muscle stumps were found to have established attachments through scar tissues to the sclera in five eyes...
December 24, 2016: International Ophthalmology
Adriano Magli, Roberta Carelli, Elisabetta Chiariello Vecchio, Francesca Esposito, Luca Rombetto, Paolo Esposito Veneruso
AIM: To evaluate long term follow-up (10y) of 6 muscle surgical approach in essential infantile esotropia (EIE). METHODS: A 6 muscle approach to EIE was retrospectively evaluated in patients with inferior oblique (IO) hyperfunction and lateral rectus (LR) pseudoparalysis, who underwent surgery at different ages. Different clinical characters were analyzed pre- and postoperatively, in patients who underwent a 6 muscles approach ≤4 years of age. All patients underwent a multiple muscles approach: bilateral medial recti (MR) recession (4-5 mm), bilateral LR resection (lower than 7 mm) and bilateral IO recession and anteroposition...
2016: International Journal of Ophthalmology
Tayyab Afghani, Hassan Mansoor
A 5-year-old boy presented with a large 5 cm × 5 cm cyst covering the left eye completely since birth. The cyst was excised in toto and was sent for histopathological examination. During the surgery, the inferior oblique (IO) muscle was seen originating from medial orbital wall, 10-12 mm behind the medial orbital margin, just posterior to the lacrimal bone and moving laterally, downward, and posteriorly from its origin making a more acute angle - around 20° to its site of origin. The insertion of the IO to sclera was at its normal site...
September 2016: Indian Journal of Ophthalmology
Gunnar Strandberg, Miklós Lipcsey, Mats Eriksson, Norbert Lubenow, Anders Larsson
BACKGROUND: Laboratory analysis of coagulation is often important in emergencies. If vascular access is challenging, intraosseous catheterization may be necessary for treatment. We studied the analysis of coagulation parameters in intraosseous aspirate during stable conditions and after major haemorrhage in a porcine model. METHODS: Ten anesthetized pigs received central venous and intraosseous catheters and samples were taken for analysis of thromboelastography (TEG), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen concentration...
November 3, 2016: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
Senthil A Kumar, Leigh C Ward, Lindsay Brown
Prebiotics alter bacterial content in the colon, and therefore could be useful for obesity management. We investigated the changes following addition of inulin oligofructose (IO) in the food of rats fed either a corn starch (C) diet or a high-carbohydrate, high-fat (H) diet as a model of diet-induced metabolic syndrome. IO did not affect food intake, but reduced body weight gain by 5·3 and 12·3 % in corn starch+inulin oligofructose (CIO) and high-carbohydrate, high-fat with inulin oligofructose (HIO) rats, respectively...
November 2016: British Journal of Nutrition
Ali Firat Cabalar, Nurullah Akbulut
Hydraulic conductivities of sands with different gradation and grain shape were estimated experimentally at a relative density (Dr) of about 40 % and a 22 ± 2 °C of constant temperature. Narli Sand (NS) with 0.67 of sphericity (S) and 0.72 of roundness (R), and Crushed Stone Sand (CSS) with 0.55 of S and 0.15 of R values were artificially graded into sixteen different grain-size fractions (4.75-2, 2-1.18, 1.18-0.6, 0.6-0.425, 0.425-0.3, 0.3-0.075, 4.75-0.075, 2-0.075, 1.18-0.075, 0.6-0.075, 0.425-0.075, 4...
2016: SpringerPlus
Thompson Kehrl, Brent A Becker, Dell E Simmons, Erin K Broderick, Robert A Jones
BACKGROUND: Intraosseous (IO) access can be complicated by obesity. Successful placement of a 25 mm IO needle is unlikely when soft tissue depth exceeds 20 mm. OBJECTIVES: The authors examined the relationship between body mass index (BMI), the ability to palpate the tibial tuberosity (TT), and soft tissue depth at recommended IO insertion sites. METHODS: Obese emergency department patients were assessed for a palpable TT and received ultrasound measurement of the soft tissue depth at recommended IO insertion sites...
September 2016: American Journal of Emergency Medicine
Garrett M Pool, Ryne A Didier, Dianna Bardo, Nathan R Selden, Anna A Kuang
OBJECTIVE The aim of this study was to develop soft-tissue and bony anthropometric orbital measurements in a normal pediatric population based upon CT scans. METHODS This was a retrospective stratified study of children with ages ranging from birth to 36 months. Head CT images for 204 children were available and obtained with 0.625-1-mm slice widths. Soft-tissue and bone windows were reviewed. Images were oriented in the Frankfort horizontal plane, and the intercanthal (IC), bony interorbital (IO), and bony lateral orbital (LO) distances were measured...
August 2016: Journal of Neurosurgery. Pediatrics
Junichiro Nakata, Hiroaki Io, Tomonari Watanabe, Yu Sasaki, Yuko Makita, Tatsuya Aoki, Hiroyuki Yanagawa, Reo Kanda, Yasuhiko Tomino
BACKGROUND: Although ultrasonography before a vascular access (VA) operation has become popular in recent years, benchmarks for the diameter or blood flow of arteries or veins are not defined in Japan. The objective of the present study is to analyze the relationship between preoperative US findings and the patency rate of VA in Japanese hemodialysis patients. METHODS: 139 patients with end stage kidney disease were enrolled in this study. They had been given primary radiocephalic arteriovenous fistula (AVF) from February 2009 to December 2010 at the Juntendo University Hospital and were followed up over 2 years...
2016: SpringerPlus
P R Bhosale, R B Iyer, P Ramalingam, K M Schmeler, W Wei, R L Bassett, P T Ramirez, M Frumovitz
AIM: To determine the ability of magnetic resonance imaging (MRI) in detecting tumour-free margins from the internal os (IO). MATERIALS AND METHODS: A database search yielded 79 women with early-stage cervical cancer who underwent radical hysterectomy and preoperative MRI. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of MRI in assessment of ≤5 and >5 mm IO involvement were calculated with histopathological surgical specimen findings considered to be the reference standard...
June 2016: Clinical Radiology
Michael Ghaly, Yong Du, Jonathan M Links, Eric C Frey
In SPECT imaging, collimators are a major factor limiting image quality and largely determine the noise and resolution of SPECT images. In this paper, we seek the collimator with the optimal tradeoff between image noise and resolution with respect to performance on two tasks related to myocardial perfusion SPECT: perfusion defect detection and joint detection and localization. We used the Ideal Observer (IO) operating on realistic background-known-statistically (BKS) and signal-known-exactly (SKE) data. The areas under the receiver operating characteristic (ROC) and localization ROC (LROC) curves (AUCd, AUCd+l), respectively, were used as the figures of merit for both tasks...
March 7, 2016: Physics in Medicine and Biology
Michael Ghaly, Jonathan M Links, Eric C Frey
The collimator is the primary factor that determines the spatial resolution and noise tradeoff in myocardial perfusion SPECT images. In this paper, the goal was to find the collimator that optimizes the image quality in terms of a perfusion defect detection task. Since the optimal collimator could depend on the level of approximation of the collimator-detector response (CDR) compensation modeled in reconstruction, we performed this optimization for the cases of modeling the full CDR (including geometric, septal penetration and septal scatter responses), the geometric CDR, or no model of the CDR...
March 7, 2016: Physics in Medicine and Biology
Vikhyat S Bebarta, Normalynn Garrett, Susan Boudreau, Maria Castaneda
OBJECTIVE: To determine if intraosseous (IO) hydroxocobalamin can improve systolic blood pressure (SBP) in a swine model after severe hemorrhagic shock. METHODS: Thirty six swine (45-55 kg) were anesthetized, intubated, and instrumented with continuous femoral and pulmonary artery pressure monitoring and then hemorrhaged such that 30 percent of their blood volume was extracted over 20 minutes. Five minutes later, animals were randomly assigned to receive 500 mL IO whole blood, 150 mg/kg IO or intravenous (IV) hydroxocobalamin in 180 mL of saline, or no treatment and then monitored for 60 minutes...
2015: American Journal of Disaster Medicine
Rick F Sanchez, Charlotte Dawson, Màrian Matas Riera, Natàlia Escanilla
OBJECTIVE: To introduce a new corneal clarity score for use in small animals and describe its inter- and intra-user variability. ANIMALS STUDIED: Twelve dogs and two cats with corneal abnormalities and five dogs with healthy corneas. MATERIALS AND METHODS: Four examiners scored every patient twice and never consecutively, focusing on the central cornea. The peripheral cornea was scored separately. The following scoring system was used to describe corneal clarity: G0: no fundus reflection is visible on retroillumination (RI) using a head-mounted indirect ophthalmoscope...
July 2016: Veterinary Ophthalmology
P K Suominen, E Nurmi, K Lauerma
Gaining vascular access in a neonate during cardiopulmonary resuscitation is crucial and challenging. Intraosseous (IO) access can offer a fast and reliable method for achieving emergency access for fluids and drugs when venous access fails in a critically ill child. IO access can however result in rare, but serious adverse events including compartment syndrome and amputation. We describe a case resulting in leg amputation due to IO infusion in a neonate after resuscitation and therapeutic hypothermia. We compared 10 tibia X-rays in three age groups...
November 2015: Acta Anaesthesiologica Scandinavica
Nkhensani Mogale, Albert-Neels van Schoor, Marius C Bosman
BACKGROUND: Studies have shown that the venous system tends to collapse during hypovolemic shock. The use of the bone marrow space for infusions is an effective alternative, with the tibial insertion site being the norm. OBJECTIVES: This study was conducted to determine a quick intraosseous infusion method that could be an alternative to the tibial route in neonates during emergency situations. METHOD: A sample of 30 neonatal cadavers was dissected to explore a possible alternative to the tibial insertion site...
2015: African Journal of Primary Health Care & Family Medicine
Ernst Michael Jung, Natascha Platz Batista da Silva, Wolfgang Jung, Stefan Farkas, Christian Stroszczynski, Janine Rennert
AIM: To evaluate the diagnostic accuracy of IO-SE in comparison to IO-CEUS for the differentiation between malignant and benign liver lesions. MATERIAL AND METHODS: In a retrospective diagnostic study IO-CEUS and SE examinations of 49 liver lesions were evaluated and compared to histopathological examinations. Ultrasound was performed using a multifrequency linear probe (6-9 MHz). The loops of CEUS were evaluated up to 5 min. The qualitative characterization of IO-SE was based on a color coding system (blue = hard, red = soft)...
2015: PloS One
Anna Fuhl, Florian Müller-Dahlhaus, Caroline Lücke, Stefan W Toennes, Ulf Ziemann
Humans liberally use ethanol for its facilitating effects on social interactions but its effects on central nervous system function remain underexplored. We have recently described that very low doses of ethanol abolish long-term potentiation (LTP)-like plasticity in human cortex, most likely through enhancement of tonic inhibition [Lücke et al, 2014, Neuropsychopharmacology 39:1508-18]. Here, we studied the effects of low-dose ethanol on long-term depression (LTD)-like plasticity. LTD-like plasticity was induced in human motor cortex by paired associative transcranial magnetic stimulation (PASLTD), and measured as decreases of motor evoked potential input-output curve (IO-curve)...
December 2015: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
Michael Ghaly, Jonathan M Links, Eric Frey
We used the ideal observer (IO) and IO with model mismatch (IO-MM) applied in the projection domain and an anthropomorphic channelized Hotelling observer (CHO) applied to reconstructed images to optimize the acquisition energy window width and to evaluate various scatter compensation methods in the context of a myocardial perfusion single-photon emission computed tomography (SPECT) defect detection task. The IO has perfect knowledge of the image formation process and thus reflects the performance with perfect compensation for image-degrading factors...
January 2015: Journal of Medical Imaging
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