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Gale Brightwell, Kylie Marree Horváth
ARDRA analysis was carried out on 90 New Zealand psychrotolerant Clostridium isolates derived from three meat production animal types and their environments. The isolates included species associated with spoilage: C. gasigenes, C. algidicarnis, C. tagluense, C. frigidicarnis and C. estertheticum. The isolates fell into 14 distinct ARDRA Groups, with 13 previously characterised meat spoilage-associated isolates shared between 6 of the 14 groups. The accuracy of ARDRA profiling analysis was supported by sequencing the 16s rRNA gene from isolates, including the representative spoilage associated Clostridium species and was consistent with previous phylogenetic relationships and classical cultural characterisation...
December 20, 2017: Meat Science
Brian J Maguire, Peter O'Meara, Barbara J O'Neill, Richard Brightwell
BACKGROUND: Violence against emergency medical services (EMS) personnel is a growing concern. The aim of this systematic review is to synthesize the current literature on violence against EMS personnel. METHODS: We examined literature from 2000 to 2016. Eligibility criteria included English-language, peer-reviewed studies of EMS personnel that described violence or assaults. Sixteen searches identified 2655 studies; 25 studies from nine countries met the inclusion criteria...
November 27, 2017: American Journal of Industrial Medicine
Andrée F Maheux, Dominique K Boudreau, Ève Bérubé, Maurice Boissinot, Frédéric Raymond, Stéphanie Brodeur, Jacques Corbeil, Gale Brightwell, Dorota Broda, Rabeea F Omar, Michel G Bergeron
The Romboutsia maritimum sp. nov. CCRI-22766(T) strain was isolated from coastal estuarine mud in New Zealand. The genome assembly comprised 2,854,352 bp, with 27.1% G+C content. This is the first documentation that reports the genome sequence of R. maritimum.
October 12, 2017: Genome Announcements
Celeste C Finnerty, Colleen F McKenna, Lauren A Cambias, Camille R Brightwell, Anesh Prasai, Ye Wang, Amina El Ayadi, David N Herndon, Oscar E Suman, Christopher S Fry
KEY POINTS: Severe burns result in significant skeletal muscle cachexia that impedes recovery. Activity of satellite cells, skeletal muscle stem cells, is altered following a burn injury and likely hinders regrowth of muscle. Severe burn injury induces satellite cell proliferation and fusion into myofibres with greater activity in muscles proximal to the injury site. Conditional depletion of satellite cells attenuates recovery of myofibre area and volume following a scald burn injury in mice...
November 1, 2017: Journal of Physiology
Chukwudubem Anibueze, Visesh Sankaran, Umar Sadat, Kelvin Tan, Yvonne G Wilson, Robert E Brightwell, Michael S Delbridge, Philip W Stather
BACKGROUND: There is no international consensus regarding the optimum management of infected aortae (mycotic aneurysms, infected aortic grafts). Neoaortoiliac reconstruction has advantages over extra-anatomical bypass grafting; however, the use of autologous vein is associated with venous hypertension and compartment syndrome, alternatively cadaveric homografts are associated with high rates of perianastomotic hemorrhage, limb occlusion, and pseudoaneurysm. Arterial repair using xenoprosthetic patches is associated with lower infection rates compared to the use of prosthetic material...
June 20, 2017: Annals of Vascular Surgery
Adrian L Cookson, Patrick J Biggs, Jonathan C Marshall, Angela Reynolds, Rose M Collis, Nigel P French, Gale Brightwell
Current culture methods to investigate changes in Escherichia coli community structure are often slow and laborious. Genes such as gnd (6-phosphogluconate dehydrogenase) have a highly variable nucleotide sequence and may provide a target for E. coli microbiome analysis using culture-independent methods. Metabarcoded PCR primers were used to generate separate libraries from calf faecal samples for high throughput sequencing. Although a total of 348 separate gnd sequence types (gSTs) were identified, 188 were likely to be due to sequencing errors...
April 12, 2017: Scientific Reports
Tanushree B Gupta, Gale Brightwell
The aim of our study was to determine the occurrence and diversity of economically important spore-forming bacteria in New Zealand dairy farm systems. Farm dairy effluent (FDE) collected from Waikato dairy farms were tested for the presence of spore-forming bacteria, using a new culture-based methodology followed by genomic analysis. An enrichment step in which samples were inoculated in cooked meat glucose starch broth under anaerobic conditions, aided in the differential isolation of Bacillus and Clostridium species...
August 2017: MicrobiologyOpen
R M Brightwell, K S Grzankowski, S Lele, K Eng, M Arshad, H Chen, K Odunsi
OBJECTIVES: The CD47 "don't eat me" signal allows tumor immune evasion. We tested the association of CD47 expression with outcomes in EOC. METHODS: CD47 expression was examined within the TCGA database for ovarian carcinoma. For validation, IHC was performed on a TMA consisting of specimens from 265 patients with EOC. The medical records of the patients were also retrospectively reviewed to correlate demographic and survival data. RESULTS: CD47 was amplified in 15/316 (5%) ovarian serous cancers in TCGA...
November 2016: Gynecologic Oncology
Rachel Brightwell, Kassondra Grzankowski, John Kasznica, Peter J Frederick
•Treatment of sex-cord stromal tumors with carboplatin and taxane is both feasible and safe.•FOXL2 mutations account for approximately 50% of these tumors.•Carboplatin and taxane may afford a favorable outcome.
November 2015: Gynecologic Oncology Reports
Brett Williams, Ted Brown, Lisa McKenna, Claire Palermo, Prue Morgan, Richard Brightwell
UNLABELLED: Because they will become health professionals of the future, it is important to assess the attitudes of students towards interprofessional education (IPE), as this will impact strongly on the development and delivery of teaching models that educators will use to facilitate IPE. The aim of this study was to examine attitudes towards, and readiness for, IPE of students from eight different undergraduate health disciplines at two Australian universities. METHODS: This cross-sectional study employed a convenience sample of first-, second-, third-, and fourth-year students enrolled in one of eight undergraduate courses from Monash University and Edith Cowan University...
2015: Journal of Allied Health
Christine Bizabani, Joanna Dames
The roots of ericaceous plants harbour a diversity of fungal taxa, which confer eco-physiological benefits to the host. Some of the fungi have been established to form ericoid mycorrhizal (ERM) associations and enhance plant growth in certain ericaceous genera. Although, Lachnum and Cadophora isolates have frequently been identified from the roots of this family, the status of their association and functional roles is still vague. The aims of this study were to identify Lachnum and Cadophora isolates; determine the root-fungal interactive structures formed in associations with Vaccinium corymbosum L...
December 2015: Microbiological Research
Marlon M Reis, Mariza G Reis, John Mills, Colleen Ross, Gale Brightwell
Confinement odour was investigated. Volatiles were extracted directly from the pack, using solid phase microextraction and analysed by gas chromatography-mass spectrometry. Sensory evaluation and microbiological analysis of the meat surface were also performed. Commercial samples of vacuum packed lamb legs (n=85), from two meat processing plants, were kept for 7weeks at -1.5°C then at different regimes of temperature (-1.5 to +4°C) until 11, 12 or 13weeks. Persistent odour was observed in 66% of samples, confinement odour in 24% and no odour in 11%...
March 2016: Meat Science
Rachel M Brightwell, Kassondra S Grzankowski, Jasmine Kaur, Samantha Poblete, Austin Miller, Shashikant B Lele, Lara Sucheston-Campbell, Kirsten Moysich, Kunle O Odunsi
Our objective was to determine whether the frequencies of non-ovarian cancers (NOC) within families in a large Familial Ovarian Cancer Registry (FOCR) are significantly different from the frequencies listed in the SEER database. The FOCR was established in 1981. Registry members are families with two or more first degree relatives who have a diagnosis of ovarian cancer, three or more cases of cancer on one side of the family with at least one being ovarian, at least one female with two or more primary cancers in which one is ovary, or a history of two or more cancers in the family with at least one being ovarian cancer diagnosed before the age of 45...
2015: American Journal of Clinical and Experimental Obstetrics and Gynecology
Fang Xi, Zheng Wang, Yong Qi, Richard Brightwell, Peter Roberts, Angus Stewart, Moira Sim, Wei Wang
OBJECTIVE: To assess the effect of respiratory training (RT) on lung function, activity tolerance and acute exacerbation frequency with chronic obstructive pulmonary disease (COPD). DESIGN: A randomised controlled trial. SETTING: Outpatient clinic and home of the COPD patients, Zhengzhou City, China. SUBJECTS: Sixty participants with COPD were randomised into two groups: an intervention group (n = 30) which received the RT in self-management and a control group (n = 30) that received an education program during the study...
December 2015: Clinical and Translational Medicine
Nonna V Kolomeyevskaya, J Brian Szender, Gary Zirpoli, Albina Minlikeeva, Grace Friel, Rikki A Cannioto, Rachel M Brightwell, Kassondra S Grzankowski, Kirsten B Moysich
OBJECTIVES: Prognostic risk factors influencing survival in patients with epithelial ovarian cancer (EOC) include tumor stage, grade, histologic subtype, debulking, and platinum status. Little is known about the impact of hormonal milieu and reproductive factors before cancer diagnosis on clinical outcome. We sought to evaluate whether oral contraceptive (OC) use carries any prognostic significance on overall survival (OS) in patients with EOC. METHODS: Newly diagnosed patients with EOC, fallopian tube, and primary peritoneal cancers between 1982 and 1998 were prospectively evaluated with a comprehensive epidemiologic questionnaire...
November 2015: International Journal of Gynecological Cancer
Kassondra S Grzankowski, Rachel M Brightwell, John M Kasznica, Kunle O Odusi
•Malignant peritoneal mesothelioma is a rare aggressive tumor with approximately 400 new cases annually in the US.•In optimal cytoreduction HIPEC is the standard treatment.•In suboptimal cytoreduction IV cisplatin and pemetrexed have high efficacy.
January 2015: Gynecologic Oncology Reports
Victor Marco Emmanuel N Ferriols, Ryoko Yaginuma, Masao Adachi, Kentaro Takada, Shigeki Matsunaga, Shigeru Okada
The diatom Rhizosolenia setigera Brightwell produces highly branched isoprenoid (HBI) hydrocarbons that are ubiquitously present in marine environments. The hydrocarbon composition of R. setigera varies between C25 and C30 HBIs depending on the life cycle stage with regard to auxosporulation. To better understand how these hydrocarbons are biosynthesized, we characterized the farnesyl pyrophosphate (FPP) synthase (FPPS) enzyme of R. setigera. An isolated 1465-bp cDNA clone contained an open reading frame spanning 1299-bp encoding a protein with 432 amino acid residues...
2015: Scientific Reports
Jessica Webb, Alexandra Brightwell, Pamela Sarkar, Roy Rabbie, Indranil Chakravorty
OBJECTIVE: To assess the uptake and impact of a peer mentoring scheme for core medical trainees on both mentors and mentees. METHOD: All second year core medical trainees in the Southwest London Training programme in September 2012 were invited to mentor a first year core medical trainee. In parallel, all first year core medical trainees were invited to be mentored. Both potential mentors and mentees were asked to submit personal statements, to attend a three-session mentoring training programme and to be matched into mentoring pairs...
April 2015: Postgraduate Medical Journal
Lara Menzies, Susan Minson, Alexandra Brightwell, Anna Davies-Muir, Andrew Long, Caroline Fertleman
OBJECTIVE: To determine if demographic factors are associated with outcome in a multiple-choice, electronically marked paediatric postgraduate examination. METHOD: Retrospective analysis of pass rates of UK trainees sitting Membership of the Royal College of Paediatrics and Child Health (MRCPCH) part 1B from 2007 to 2011. Data collected by the RCPCH from examination candidates were analysed to assess the effects of gender, age, and country and university of medical qualification on examination outcome...
February 2015: Postgraduate Medical Journal
Sara Brightwell, Keisuke Kaji
Generation of iPSCs is inefficient and the molecular mechanisms underlying reprogramming are not well understood. While several studies have demonstrated that reprogramming is not entirely a random process and contains predictable stepwise changes, varying degrees of cellular heterogeneity that arise in different reprogramming systems can obscure the process. Among several reprogramming systems available, delivery of polycistronic reprogramming factor expression cassettes with piggyBac transposon into mouse embryonic fibroblasts (MEFs) is one of the simplest and most robust reprogramming approaches that provide a low background of partially reprogrammed cells...
2016: Methods in Molecular Biology
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