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https://www.readbyqxmd.com/read/28642109/neoaortic-xenoprosthetic-grafts-for-treatment-of-mycotic-aneurysms-and-infected-aortic-grafts
#1
C Anibueze, V Sankaran, U Sadat, K Tan, Y G Wilson, R E Brightwell, M S Delbridge, P W Stather
OBJECTIVE: There is no international consensus regarding the optimum management of infected aortae (mycotic aneurysms, infected aortic grafts). Neoaortoiliac reconstruction has advantages over extra anatomical bypass grafting; however use of autologous vein is associated with venous hypertension and compartment syndrome, alternatively cadaveric homografts are associated with high rates of perianastomotic haemorrhage, limb occlusion and pseudoaneurysm. Arterial repair using xenoprosthetic patches is associated with lower infection rates compared to the use of prosthetic material...
June 19, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28404985/culture-independent-analysis-using-gnd-as-a-target-gene-to-assess-escherichia-coli-diversity-and-community-structure
#2
Adrian L Cookson, Patrick J Biggs, Jonathan C Marshall, Angela Reynolds, Rose M Collis, Nigel P French, Gale Brightwell
Current culture methods to investigate changes in Escherichia coli community structure are often slow and laborious. Genes such as gnd (6-phosphogluconate dehydrogenase) have a highly variable nucleotide sequence and may provide a target for E. coli microbiome analysis using culture-independent methods. Metabarcoded PCR primers were used to generate separate libraries from calf faecal samples for high throughput sequencing. Although a total of 348 separate gnd sequence types (gSTs) were identified, 188 were likely to be due to sequencing errors...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28256808/farm-level-survey-of-spore-forming-bacteria-on-four-dairy-farms-in-the-waikato-region-of-new-zealand
#3
Tanushree B Gupta, Gale Brightwell
The aim of our study was to determine the occurrence and diversity of economically important spore-forming bacteria in New Zealand dairy farm systems. Farm dairy effluent (FDE) collected from Waikato dairy farms were tested for the presence of spore-forming bacteria, using a new culture-based methodology followed by genomic analysis. An enrichment step in which samples were inoculated in cooked meat glucose starch broth under anaerobic conditions, aided in the differential isolation of Bacillus and Clostridium species...
March 3, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/27569584/the-cd47-don-t-eat-me-signal-is-highly-expressed-in-human-ovarian-cancer
#4
R M Brightwell, K S Grzankowski, S Lele, K Eng, M Arshad, H Chen, K Odunsi
OBJECTIVES: The CD47 "don't eat me" signal allows tumor immune evasion. We tested the association of CD47 expression with outcomes in EOC. METHODS: CD47 expression was examined within the TCGA database for ovarian carcinoma. For validation, IHC was performed on a TMA consisting of specimens from 265 patients with EOC. The medical records of the patients were also retrospectively reviewed to correlate demographic and survival data. RESULTS: CD47 was amplified in 15/316 (5%) ovarian serous cancers in TCGA...
November 2016: Gynecologic Oncology
https://www.readbyqxmd.com/read/26793762/poorly-differentiated-sertoli-leydig-tumor-with-heterologous-high-grade-sarcomatoid-features-a-case-report
#5
Rachel Brightwell, Kassondra Grzankowski, John Kasznica, Peter J Frederick
•Treatment of sex-cord stromal tumors with carboplatin and taxane is both feasible and safe.•FOXL2 mutations account for approximately 50% of these tumors.•Carboplatin and taxane may afford a favorable outcome.
November 2015: Gynecologic Oncology Reports
https://www.readbyqxmd.com/read/26661698/students-attitudes-toward-interprofessional-learning-a-comparison-between-two-universities
#6
Brett Williams, Ted Brown, Lisa McKenna, Claire Palermo, Prue Morgan, Richard Brightwell
UNLABELLED: Because they will become health professionals of the future, it is important to assess the attitudes of students towards interprofessional education (IPE), as this will impact strongly on the development and delivery of teaching models that educators will use to facilitate IPE. The aim of this study was to examine attitudes towards, and readiness for, IPE of students from eight different undergraduate health disciplines at two Australian universities. METHODS: This cross-sectional study employed a convenience sample of first-, second-, third-, and fourth-year students enrolled in one of eight undergraduate courses from Monash University and Edith Cowan University...
2015: Journal of Allied Health
https://www.readbyqxmd.com/read/26640054/effects-of-inoculating-lachnum-and-cadophora-isolates-on-the-growth-of-vaccinium-corymbosum
#7
Christine Bizabani, Joanna Dames
The roots of ericaceous plants harbour a diversity of fungal taxa, which confer eco-physiological benefits to the host. Some of the fungi have been established to form ericoid mycorrhizal (ERM) associations and enhance plant growth in certain ericaceous genera. Although, Lachnum and Cadophora isolates have frequently been identified from the roots of this family, the status of their association and functional roles is still vague. The aims of this study were to identify Lachnum and Cadophora isolates; determine the root-fungal interactive structures formed in associations with Vaccinium corymbosum L...
December 2015: Microbiological Research
https://www.readbyqxmd.com/read/26624794/characterization-of-volatile-metabolites-associated-with-confinement-odour-during-the-shelf-life-of-vacuum-packed-lamb-meat-under-different-storage-conditions
#8
Marlon M Reis, Mariza G Reis, John Mills, Colleen Ross, Gale Brightwell
Confinement odour was investigated. Volatiles were extracted directly from the pack, using solid phase microextraction and analysed by gas chromatography-mass spectrometry. Sensory evaluation and microbiological analysis of the meat surface were also performed. Commercial samples of vacuum packed lamb legs (n=85), from two meat processing plants, were kept for 7weeks at -1.5°C then at different regimes of temperature (-1.5 to +4°C) until 11, 12 or 13weeks. Persistent odour was observed in 66% of samples, confinement odour in 24% and no odour in 11%...
March 2016: Meat Science
https://www.readbyqxmd.com/read/26605374/higher-than-expected-frequencies-of-non-ovarian-cancers-within-a-large-familial-ovarian-cancer-registry
#9
Rachel M Brightwell, Kassondra S Grzankowski, Jasmine Kaur, Samantha Poblete, Austin Miller, Shashikant B Lele, Lara Sucheston-Campbell, Kirsten Moysich, Kunle O Odunsi
Our objective was to determine whether the frequencies of non-ovarian cancers (NOC) within families in a large Familial Ovarian Cancer Registry (FOCR) are significantly different from the frequencies listed in the SEER database. The FOCR was established in 1981. Registry members are families with two or more first degree relatives who have a diagnosis of ovarian cancer, three or more cases of cancer on one side of the family with at least one being ovarian, at least one female with two or more primary cancers in which one is ovary, or a history of two or more cancers in the family with at least one being ovarian cancer diagnosed before the age of 45...
2015: American Journal of Clinical and Experimental Obstetrics and Gynecology
https://www.readbyqxmd.com/read/26458936/long-term-effect-of-respiratory-training-for-chronic-obstructive-pulmonary-disease-patients-at-an-outpatient-clinic-a-randomised-controlled-trial
#10
Fang Xi, Zheng Wang, Yong Qi, Richard Brightwell, Peter Roberts, Angus Stewart, Moira Sim, Wei Wang
OBJECTIVE: To assess the effect of respiratory training (RT) on lung function, activity tolerance and acute exacerbation frequency with chronic obstructive pulmonary disease (COPD). DESIGN: A randomised controlled trial. SETTING: Outpatient clinic and home of the COPD patients, Zhengzhou City, China. SUBJECTS: Sixty participants with COPD were randomised into two groups: an intervention group (n = 30) which received the RT in self-management and a control group (n = 30) that received an education program during the study...
December 2015: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/26273932/oral-contraceptive-use-and-reproductive-characteristics-affect-survival-in-patients-with-epithelial-ovarian-cancer-a-cohort-study
#11
Nonna V Kolomeyevskaya, J Brian Szender, Gary Zirpoli, Albina Minlikeeva, Grace Friel, Rikki A Cannioto, Rachel M Brightwell, Kassondra S Grzankowski, Kirsten B Moysich
OBJECTIVES: Prognostic risk factors influencing survival in patients with epithelial ovarian cancer (EOC) include tumor stage, grade, histologic subtype, debulking, and platinum status. Little is known about the impact of hormonal milieu and reproductive factors before cancer diagnosis on clinical outcome. We sought to evaluate whether oral contraceptive (OC) use carries any prognostic significance on overall survival (OS) in patients with EOC. METHODS: Newly diagnosed patients with EOC, fallopian tube, and primary peritoneal cancers between 1982 and 1998 were prospectively evaluated with a comprehensive epidemiologic questionnaire...
November 2015: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/26076085/malignant-peritoneal-mesothelioma-without-asbestos-exposure-an-ovarian-cancer-imitator
#12
Kassondra S Grzankowski, Rachel M Brightwell, John M Kasznica, Kunle O Odusi
•Malignant peritoneal mesothelioma is a rare aggressive tumor with approximately 400 new cases annually in the US.•In optimal cytoreduction HIPEC is the standard treatment.•In suboptimal cytoreduction IV cisplatin and pemetrexed have high efficacy.
January 2015: Gynecologic Oncology Reports
https://www.readbyqxmd.com/read/25996801/cloning-and-characterization-of-farnesyl-pyrophosphate-synthase-from-the-highly-branched-isoprenoid-producing-diatom-rhizosolenia-setigera
#13
Victor Marco Emmanuel N Ferriols, Ryoko Yaginuma, Masao Adachi, Kentaro Takada, Shigeki Matsunaga, Shigeru Okada
The diatom Rhizosolenia setigera Brightwell produces highly branched isoprenoid (HBI) hydrocarbons that are ubiquitously present in marine environments. The hydrocarbon composition of R. setigera varies between C25 and C30 HBIs depending on the life cycle stage with regard to auxosporulation. To better understand how these hydrocarbons are biosynthesized, we characterized the farnesyl pyrophosphate (FPP) synthase (FPPS) enzyme of R. setigera. An isolated 1465-bp cDNA clone contained an open reading frame spanning 1299-bp encoding a protein with 432 amino acid residues...
2015: Scientific Reports
https://www.readbyqxmd.com/read/25715334/peer-mentoring-for-core-medical-trainees-uptake-and-impact
#14
Jessica Webb, Alexandra Brightwell, Pamela Sarkar, Roy Rabbie, Indranil Chakravorty
OBJECTIVE: To assess the uptake and impact of a peer mentoring scheme for core medical trainees on both mentors and mentees. METHOD: All second year core medical trainees in the Southwest London Training programme in September 2012 were invited to mentor a first year core medical trainee. In parallel, all first year core medical trainees were invited to be mentored. Both potential mentors and mentees were asked to submit personal statements, to attend a three-session mentoring training programme and to be matched into mentoring pairs...
April 2015: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/25617382/an-evaluation-of-demographic-factors-affecting-performance-in-a-paediatric-membership-multiple-choice-examination
#15
Lara Menzies, Susan Minson, Alexandra Brightwell, Anna Davies-Muir, Andrew Long, Caroline Fertleman
OBJECTIVE: To determine if demographic factors are associated with outcome in a multiple-choice, electronically marked paediatric postgraduate examination. METHOD: Retrospective analysis of pass rates of UK trainees sitting Membership of the Royal College of Paediatrics and Child Health (MRCPCH) part 1B from 2007 to 2011. Data collected by the RCPCH from examination candidates were analysed to assess the effects of gender, age, and country and university of medical qualification on examination outcome...
February 2015: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/25410291/piggybac-transposon-mediated-reprogramming-and-flow-cytometry-analysis-of-cd44-and-icam1-cell-surface-marker-changes
#16
Sara Brightwell, Keisuke Kaji
Generation of iPSCs is inefficient and the molecular mechanisms underlying reprogramming are not well understood. While several studies have demonstrated that reprogramming is not entirely a random process and contains predictable stepwise changes, varying degrees of cellular heterogeneity that arise in different reprogramming systems can obscure the process. Among several reprogramming systems available, delivery of polycistronic reprogramming factor expression cassettes with piggyBac transposon into mouse embryonic fibroblasts (MEFs) is one of the simplest and most robust reprogramming approaches that provide a low background of partially reprogrammed cells...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/25350929/changes-in-temperature-affect-the-risk-of-abdominal-aortic-aneurysm-rupture
#17
Robert E Brightwell, Andrew M T L Choong, Adrian G Barnett, Philip J Walker
BACKGROUND: There is a well-established link between exposure to hot and cold temperatures and an increased risk of cardiovascular hospitalization or death. There is also contrasting evidence of a seasonal increase in aortic ruptures related to atmospheric pressure, but an association with environmental temperature has never been formally modelled. METHODS: Using a prospective database, we identified 295 patients who were operated in a single centre for ruptured abdominal aortic aneurysm in south-east Queensland between 1990 and 2010...
November 2014: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/24875594/factors-affecting-microbial-spoilage-and-shelf-life-of-chilled-vacuum-packed-lamb-transported-to-distant-markets-a-review
#18
REVIEW
John Mills, Andrea Donnison, Gale Brightwell
Vacuum-packaging and stringent control of storage temperatures enable the export of meat to distant markets, supplying a chilled product that can favourably compete with local fresh meats. To save fuel and reduce emissions, the speed of ships travelling to international markets has decreased resulting in requirement for the shelf-life of chilled lamb to be extended beyond the recognised time of 60-70 days. Growth of microorganisms and ability to cause spoilage of vacuum-packed lamb are dependent on many factors, including the type and initial concentration of spoilage bacteria, meat pH, water activity, availability of substrates, oxygen availability and, most importantly, storage time and temperature of the packaged product...
September 2014: Meat Science
https://www.readbyqxmd.com/read/24833947/empathy-levels-among-health-professional-students-a-cross-sectional-study-at-two-universities-in-australia
#19
Brett Williams, Ted Brown, Lisa McKenna, Malcolm J Boyle, Claire Palermo, Debra Nestel, Richard Brightwell, Louise McCall, Verity Russo
BACKGROUND: Empathy is paramount in the health care setting, optimizing communication and rapport with patients. Recent empirical evidence suggests that empathy is associated with improved clinical outcomes. Therefore, given the importance of empathy in the health care setting, gaining a better understanding of students' attitudes and self-reported empathy is important. The objective of this study was to examine self-reported empathy levels of students enrolled in different health disciplines from two large Australian universities...
2014: Advances in Medical Education and Practice
https://www.readbyqxmd.com/read/24821548/episodic-seasonal-pseudo-bartter-syndrome-in-cystic-fibrosis
#20
Brett Kintu, Alex Brightwell
Pseudo-Bartter syndrome (PBS) describes an uncommon but well recognised complication of cystic fibrosis leading to hypochloraemic, hypokalaemic metabolic alkalosis. Pseudo-Bartter syndrome is usually seen at initial presentation or within the first two years of life in children with cystic fibrosis. Risk factors for development of PBS include warm weather conditions, severe respiratory or pancreatic disease and gastrointestinal losses (e.g. vomiting and diarrhoea). PBS is rare in older children and adolescents although epidemics have been associated with heat wave conditions in warmer climates...
June 2014: Paediatric Respiratory Reviews
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