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https://www.readbyqxmd.com/read/29329754/comparison-of-cepheid%C3%A2-xpert-flu-and-roche-realtime-ready-influenza-a-h1n1-detection-set-for-detection-of-influenza-a-h1n1
#1
Ali A Rabaan, Ali M Bazzi, Sana A Alshaikh
OBJECTIVE: To compare two influenza polymerase chain reaction (PCR) methods. METHODS: A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay. RESULTS: There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95...
December 16, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29240781/comparing-mail-in-self-collected-specimens-sent-via-united-states-postal-service-versus-clinic-collected-specimens-for-the-detection-of-chlamydia-trachomatis-and-neisseria-gonorrhoeae-in-extra-genital-sites
#2
Katheryn R Salow, Adam C Cohen, Claire C Bristow, Mark R McGrath, Jeffrey D Klausner
OBJECTIVES: To evaluate the concordance between clinic-collected extra-genital specimens and self-collected mailed-in extra-genital specimens among participants seeking sexually transmitted infection testing at a free clinic in Hollywood, CA. METHODS: A convenience sample of 210 men who have sex with men were enrolled between February 29, 2016 and December 21, 2016 and received mail-in testing kits for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG). All testing was performed using the GeneXpert® CT/NG (Cepheid, Sunnyvale, CA)...
2017: PloS One
https://www.readbyqxmd.com/read/29174733/comparison-of-the-cepheid-genexpert-ct-ng-assay-to-the-hologic-aptima-combo2-assay-for-the-detection-of-chlamydia-trachomatis-and-neisseria-gonorrhoeae-in-self-collected-rectal-swabs
#3
Laura Dize, Barbara Silver, Charlotte Gaydos
Self-collected rectal-swabs were tested for CT and NG on GeneXpert CT/NG as compared to APTIMA Combo2. Of 448 rectal-swabs, 22 were positive for CT; 7 for NG on both assays; two were discordant. Sensitivity and specificity of GeneXpert was 95.5% and 99.7% for chlamydia, respectively; for gonorrhea both were 100%.
October 26, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29167292/clinical-evaluation-of-the-cepheid-xpert%C3%A2-tv-assay-for-detection-of-trichomonas-vaginalis-with-prospectively-collected-female-and-male-specimens
#4
Jane R Schwebke, C A Gaydos, T Davis, J Marrazzo, D Furgerson, S N Taylor, B Smith, L H Bachmann, R Ackerman, T Spurrell, D Ferris, C A Burnham, H Reno, J Lebed, D Eisenberg, P Kerndt, S Philip, J Jordan, N Quigley
Trichomoniasis is the most prevalent curable sexually transmitted disease (STD). It has been associated with preterm birth and acquisition/transmission of HIV. Recently, nucleic acid amplification tests (NAAT) have been FDA-cleared in the United States for detection of Trichomonas vaginalis (TV) in specimens from both women and men. This current study reports the results of a multicenter study recently conducted using the Xpert TV Assay to test specimens from both men and women. On-demand results were available in as little as 40 minutes for positive specimens...
November 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29142048/accurate-pcr-detection-of-influenza-a-b-and-rsv-using-the-cepheid-xpert-flu-rsv-xpress-assay-in-point-of-care-settings-comparison-to-prodesse-proflu
#5
Daniel M Cohen, Jennifer Kline, Larissa S May, Glenn Eric Harnett, Jane Gibson, Stephen Y Liang, Zubaid Rafique, Carina A Rodriguez, Kevin M McGann, Charlotte A Gaydos, Donna Mayne, David Phillips, Jason Cohen
The Xpert Flu+RSV Xpress Assay is a fast, automated in vitro diagnostic test for qualitative detection and differentiation of influenza A, B, and RSV performed on the Cepheid GeneXpert Xpress System. The objective of this study was to establish performance characteristics of the Xpert Flu+RSV Xpress Assay compared to the Prodesse ProFlu+ real-time RT-PCR assay (ProFlu+) for the detection of influenza A and B as well as RSV in a CLIA-waived (CW) setting. Overall, the assay, using fresh and frozen nasopharyngeal (NP) swabs, demonstrated high concordance with ProFlu+ in the combined CW and non-CW settings with Positive Percent Agreements (PPA) (100%, 100%, 97...
November 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29133554/what-is-resistance-impact-of-phenotypic-versus-molecular-drug-resistance-testing-on-multi-and-extensively-drug-resistant-tuberculosis-therapy
#6
Jan Heyckendorf, Sönke Andres, Claudio U Köser, Ioana D Olaru, Thomas Schön, Erik Sturegård, Patrick Beckert, Viola Schleusener, Thomas A Kohl, Doris Hillemann, Danesh Moradigaravand, Julian Parkhill, Sharon J Peacock, Stefan Niemann, Christoph Lange, Matthias Merker
Rapid and accurate drug-susceptibility testing (DST) is essential for the treatment of multi- and extensively drug-resistant tuberculosis (M/XDR-TB). We compared the utility of genotypic DST assays with phenotypic DST (pDST) using BACTEC 960 MGIT or Löwenstein-Jensen to construct M/XDR-TB treatment regimens for a cohort of 25 consecutive M/XDR-TB patients and 15 possible anti-TB drugs.Genotypic DST results from Cepheid GeneXpert MTB/RIF (Xpert) and line probe assays (LPAs: Hain GenoType MTBDRplus 2.0 and MTBDRsl 2...
November 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29091135/an-evaluation-of-false-positive-rifampicin-resistance-on-the-xpert-mtb-rif
#7
Yeliz Tanriverdi Cayci, Kemal Bilgin, Ahmet Yilmaz Coban, Asuman Birinci, Belma Durupınar
BACKGROUND: Mycobacterium tuberculosis (MTB) is one of the most significant causes of mortality and morbidity. Early diagnose is important especially in multiple drug resistant tuberculosis to avoid transmission. Traditional techniques requires at least one to three weeks for diagnosis of tuberculosis. Diagnostic delays with multiple drug resistant tuberculosis are associated with worse clinical outcomes and increased transmission The Xpert MTB/RIF assay is one of the new diagnostic device for the diagnosis of tuberculosis and rapid detection of rifampicin resistance...
November 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29082442/intrapartum-group-b-streptococcus-screening-in-the-labor-ward-by-xpert%C3%A2-gbs-real-time-pcr
#8
C Plainvert, F El Alaoui, A Tazi, C Joubrel, O Anselem, M Ballon, A Frigo, C Branger, L Mandelbrot, F Goffinet, C Poyart
Group B Streptococcus (GBS) is the leading cause of neonatal infections in industrialized countries. Intrapartum antibiotic prophylaxis (IAP) given to colonized parturients is a key step for the prevention of neonatal early-onset infection. We compared the performances of Xpert® GBS polymerase chain reaction (PCR) (Cepheid, Sunnyvale, CA, USA) as a point-of-care system in labor wards to standard culture for intrapartum GBS detection. Pregnant women with a GBS-positive antenatal screening were prospectively included...
October 29, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29074186/xpert-human-papillomavirus-test-is-a-promising-cervical-cancer-screening-test-for-hiv-seropositive-women
#9
Zizipho Z A Mbulawa, Timothy J Wilkin, Bridgette Goeieman, Avril Swarts, Sophie Williams, Simon Levin, Mark Faesen, Jennifer S Smith, Carla J Chibwesha, Anna-Lise Williamson, Cynthia Firnhaber
This study investigated the performance of Cepheid Xpert human papillomavirus (HPV) assay in South African human immunodeficiency virus (HIV)-infected women and compared its performance with that of hybrid capture-2 (hc2). METHODS: Stored cervical specimens from HIV-infected women that had previously been tested using hc2 were tested using Xpert. RESULTS: The overall HR-HPV prevalence was found to be 62.0% (720/1161) by Xpert and 61.2% (711/1161) by hc2. 13.6% (158/1161) were HPV16 positive, 18...
December 2016: Papillomavirus Research
https://www.readbyqxmd.com/read/29074030/reliable-rapid-assay-for-gonorrhea-and-chlamydia-in%C3%A2-the%C3%A2-emergency-department
#10
Sean P Wilson, Taher Vohra, Jared Goldberg, Christopher Price, Sean Calo, Meredith Mahan, Joseph Miller
BACKGROUND: Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are common sexually transmitted infections seen in the emergency department (ED). Due to an inability to reliably make accurate diagnosis by physical examination, concern for unreliable follow-up, and current delays in diagnostic nucleic acid amplification testing (NAAT), presumptive treatment active against CT and NG, as described by Centers for Disease Control clinical practice guidelines, is often performed. OBJECTIVES: The purpose of this study was to determine whether a rapid, urine NAAT performed in the ED is noninferior in its diagnostic sensitivity compared with a traditional, swab NAAT assay...
October 23, 2017: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29073731/performance-evaluation-of-cepheid-xpert-norovirus-kit-with-a-user-modified-protocol
#11
Rachel Shi-Lei Wong, Fion Yeo, Wai Theng Chia, Chun Kiat Lee, Mun Han Leong, Christopher Wai-Siong Ng, Kok Siong Poon, Gabriel Zherong Yan, Lily-Lily Chiu, Benedict Junrong Yan, Roland Jureen, Evelyn Siew-Chuan Koay, Hong Kai Lee
The Cepheid Xpert® Norovirus kit automates sample processing, nucleic acid extraction, and real-time reverse transcription polymerase chain reactions (RT-PCRs) to detect norovirus genogroups I (GI) and II (GII). Eighty-five stool samples collected between February 2015 and May 2017 were used to compare the performance of a user-modified Xpert assay against a clinically validated laboratory-developed test (LDT). Of the 85 samples, 54 were previously archived in -80°C freezer. The remaining 31 were fresh samples tested concurrently with the LDT...
October 26, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29066627/detection-of-neisseria-gonorrhoeae-and-chlamydia-trachomatis-from-pooled-rectal-pharyngeal-and-urine-specimens-in-men-who-have-sex-with-men
#12
David John Speers, I-Ly Joanna Chua, Justin Manuel, Lewis Marshall
OBJECTIVES: Screening of men who have sex with men (MSM) for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) requires sampling from anorectal and pharyngeal sites in addition to urogenital sampling. Due to the cost of testing multiple anatomical sites individually testing of pooled specimens has potential merit. The Cepheid GeneXpert CT/NG assay (GeneXpert), which also has potential for point-of-care nucleic acid testing in the sexual health clinic, has not been assessed for pooled specimen testing...
October 24, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29061538/development-of-a-90-minute-integrated-noninvasive-urinary-assay-for-bladder-cancer-detection
#13
Ellen Wallace, Russell Higuchi, Malini Satya, Leena McCann, Mandy L Y Sin, Julia A Bridge, Huilin Wei, Jun Zhang, Edith Wong, Andrew Hiar, Kathleen E Mach, Douglas Scherr, R Blair Egerdie, Shoichiro Ohta, Wade J Sexton, Maxwell V Meng, Alon Z Weizer, Michael Woods, G Kenneth Jansz, Joseph Zadra, Yair Lotan, Bernard Goldfarb, Joseph C Liao
PURPOSE: Despite suboptimal sensitivity urine cytology is often performed as an adjunct to cystoscopy for bladder cancer diagnosis. We aimed to develop a noninvasive, fast molecular diagnostic test for bladder cancer detection with better sensitivity than urine cytology while maintaining adequate specificity. MATERIALS AND METHODS: Urine specimens were collected at 18 multinational sites from subjects prior to cystoscopy or tumor resection and from healthy and other control subjects without evidence of bladder cancer...
October 20, 2017: Journal of Urology
https://www.readbyqxmd.com/read/29057945/a-pilot-evaluation-of-whole-blood-finger-prick-sampling-for-point-of-care-hiv-viral-load-measurement-the-unicorn-study
#14
Sarah Fidler, Heather Lewis, Jodi Meyerowitz, Kristin Kuldanek, John Thornhill, David Muir, Alice Bonnissent, Georgina Timson, John Frater
There is a global need for HIV viral load point-of-care (PoC) assays to monitor patients receiving antiretroviral therapy. UNICORN was the first study of an off-label protocol using whole blood finger-prick samples tested with and without a simple three minute spin using a clinic-room microcentrifuge. Two PoC assays were evaluated in 40 HIV-positive participants, 20 with detectable and 20 with undetectable plasma viral load (pVL) (<20 copies/ml). Using 100 µl finger-prick blood samples, the Cepheid Xpert HIV-1 Viral Load and HIV-1 Qual cartridges were compared with laboratory pVL assessment (TaqMan, Roche)...
October 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29053085/rifampicin-resistance-and-multidrug-resistant-tuberculosis-detection-using-xpert-mtb-rif-in-wuhan-china-a-retrospective-study
#15
Hai Huang, Yanlin Zhang, Sheng Li, Jun Wang, Jun Chen, Zhiyun Pan, Hui Gan
BACKGROUND: The Xpert MTB/RIF test (Cepheid, Sunnyvale, CA) can simultaneously detect the Mycobacterium tuberculosis (MTB) complex DNA and rifampicin (RFP) resistance and can rapidly determine RFP resistance and predict multidrug-resistant tuberculosis (MDR-TB). In this study, we analyzed clinical examination results of a hospital specializing in TB treatment in Wuhan, Hubei, China, and examined the use of traditional culture and drug-sensitive test (DST) results as a gold standard to assess the diagnosis value of the Xpert MTB/RIF test in RFP resistance and MDR-TB...
October 20, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29036487/prospective-and-retrospective-evaluation-of-the-performance-of-the-fda-approved-cepheid-xpert-flu-rsv-xc-assay
#16
Sophie Arbefeville, Elizabeth Thonen-Kerr, Patricia Ferrieri
Rapid and accurate detection of respiratory viruses is important in patient care and in guiding therapy and infection prevention policy. Rapid viral antigen assays are simple to perform and provide results within 15 to 30 minutes but are limited by their modest-to-moderate sensitivity. Molecular assays are more sensitive and specific but require more technical time and expertise and are more expensive. We verified the performance of the Xpert Flu/RSV XC assay prospectively, using patient respiratory samples from the 2014-2015 respiratory season, and, retrospectively, with frozen patient samples from the previous respiratory season...
November 8, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/28966035/comparison-of-three-molecular-assays-for-detection-of-enteric-viruses-in-stool-samples
#17
Haciba Moudjahed, Claire Pinçon, Kazali Alidjinou, Anny Dewilde, Anne Goffard
Three molecular assays (FTD(®) Viral GE from Fast-track diagnostics, RIDA(®)GENE VSP1 from R-Biopharm, and Xpert Norovirus from Cepheid) were compared for virus detection in acute diarrhea samples. RIDA(®)GENE and FTD(®) Viral GE showed perfect/almost perfect agreement for Rotavirus, Sapovirus and Norovirus, substantial agreement for Adenovirus, and moderate agreement for Astrovirus.
September 29, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28956800/molecular-characterization-of-neisseria-gonorrhoeae-on-non-cultured-specimens-from-multiple-anatomic-sites
#18
Anna Carannante, Valeria Ghisetti, Ivano Dal Conte, Gabriella Gregori, Maria Laura Stella, Paola Vacca, Simonetta Del Re, Paola Stefanelli
INTRODUCTION: The aim of this study was to molecularly characterize Neisseria gonorrhoeae on non-cultured specimens collected from multiple anatomic sites. N. gonorrhoeae multiantigen sequence typing (NG-MAST) together with the gene sequence analysis of antimicrobial resistance (AMR) target genes were used. MATERIALS AND METHODS: Seventeen genital and extra-genital samples from eight patients (7 were men who have sex with men, MSM, and 1 women who have sex with men, WSM) with gonorrhoea symptoms were analyzed...
July 2017: Annali Dell'Istituto Superiore di Sanità
https://www.readbyqxmd.com/read/28954901/evaluation-of-the-cobas%C3%A2-cdiff-test-for-the-detection-of-toxigenic-clostridium-difficile-in-stool-samples
#19
Lance R Peterson, Stephen A Young, Thomas E Davis, Zi-Xuam Wang, John Duncan, Christopher Noutsios, Oliver Liesenfeld, John C Osiecki, Michael A Lewinski
Nucleic acid amplification tests are reliable tools for the detection of toxigenic Clostridium difficile from unformed (liquid or soft) stool samples. The objective of this study was to evaluate performance of the cobas® Cdiff Test on the cobas® 4800 System using prospectively collected stool specimens from patients suspected of C. difficile infection (CDI). Performance of the cobas® Cdiff Test was compared to the combined results of direct and broth enriched toxigenic culture in a large, multicenter clinical trial...
September 27, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28920845/modified-use-of-real-time-pcr-detection-of-group-b-streptococcus-in-pregnancy
#20
Ali A Rabaan, Justin V Saunar, Ali M Bazzi, Joan L Soriano
The CDC recommends antenatal screening of vaginal/rectal samples for Streptococcus agalactiae at 35-37 weeks' gestation, with intra-partum antibiotic prophylaxis for positive cases. We tested a modified use of the Cepheid Xpert GBS real-time PCR kit on enrichment cultures from 554 vaginal/rectal swabs compared to the current subculturing gold standard method. Swabs were inoculated on polymyxin nalidixic acid agar plates, and Todd-Hewitt enrichment broth cultures were examined daily for growth. Todd-Hewitt broth culture was also used for Xpert GBS...
October 2017: Journal of Medical Microbiology
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