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https://www.readbyqxmd.com/read/27919639/-evaluation-of-an-xpert-ev-cepheid-%C3%A2-molecular-diagnostic-technique-for-enteroviral-meningitis
#1
Natalia Alonso Pérez, Belén Sagastizabal Cardelus, Luis Manuel Prieto Tato, Sara Guillén Martín, Ana González Torralba, Isabel García Bermejo, José Tomás Ramos Amador
INTRODUCTION: Polymerase chain reaction (PCR) assays have shown to be useful and quick for the diagnosis of enterovirus in aseptic meningitis. The aim of our study was to analyse the changes in clinical practice after the introduction of a real-time polymerase chain reaction (RT-PCR) technique using the Xpert EV (Cepheid(®)) assay for the qualitative detection of enterovirus RNA in cerebrospinal fluid specimens from children with suspected viral meningitis. METHODS: A retrospective study was performed in children older than 1year, diagnosed with enterovirus meningitis in a third level hospital from November 2006 to February 2013...
December 2, 2016: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
https://www.readbyqxmd.com/read/27898567/utilization-of-the-cepheid-xpert%C3%A2-ct-ng-sample-adequacy-control-to-determine-the-influence-of-the-urethral-swab-on-cellular-content-in-post-swab-versus-pre-swab-urine
#2
Stephen J Jordan, Barbara Van Der Pol, Edward W Hook
Chlamydia trachomatis/Neisseria gonorrhoeae assay performance in males is typically determined using post-swab urine, though pre-swab urine is used in practice. We collected swabs and urine from men and used the Cepheid Xpert® CT/NG sample adequacy control to determine the effect of swab collection on urine cellular content. No difference was observed.
November 28, 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27891336/mycobacterium-tuberculosis-beijing-genotype-in-western-iran-distribution-and-drug-resistance
#3
Parviz Mohajeri, Sakineh Moradi, Sara Atashi, Abbas Farahani
INTRODUCTION: Mycobacterium tuberculosis Beijing genotype is gaining importance all over the world because this genotype is highly prevalent in several areas and is also frequently associated with drug resistance. AIM: To identify and determine the frequency of Beijing genotype and mix infection with Beijing and non-Beijing in west of Iran and analyse the association between Beijing genotype and drug resistance. MATERIALS AND METHODS: This cross-sectional study was conducted on 146 Tuberculosis (TB) samples collected at the TB reference laboratory in Kermanshah west of Iran from January 2014 to February 2015, Mycobacterium tuberculosis isolates from sputum samples, detected by microcopy, biochemical tests and solid culture were included and then the confirmed samples with Cepheid Xpert MTB/RIF assay were subjected to drug susceptibility tests for rifampicin, isoniazid, ethambutol using proportional method...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27865383/analytical-and-clinical-performance-characteristics-of-the-abbott-realtime-mtb-rif-inh-resistance-an-assay-for-the-detection-of-rifampicin-and-isoniazid-resistant-mycobacterium-tuberculosis-in-pulmonary-specimens
#4
Joshua Kostera, Gregor Leckie, Ning Tang, John Lampinen, Magdalena Szostak, Klara Abravaya, Hong Wang
Clinical management of drug-resistant tuberculosis patients continues to present significant challenges to global health. To tackle these challenges, the Abbott RealTime MTB RIF/INH Resistance assay was developed to accelerate the diagnosis of rifampicin and/or isoniazid resistant tuberculosis to within a day. This article summarizes the performance of the Abbott RealTime MTB RIF/INH Resistance assay; including reliability, analytical sensitivity, and clinical sensitivity/specificity as compared to Cepheid GeneXpert MTB/RIF version 1...
December 2016: Tuberculosis
https://www.readbyqxmd.com/read/27846818/asymptomatic-and-yet-c-difficile-toxin-positive-prevalence-and-risk-factors-of-carriers-of-toxigenic-clostridium-difficile-among-geriatric-in-patients
#5
Klaus Nissle, Daniel Kopf, Alexander Rösler
BACKGROUND: Clostridium difficile infections (CDI) are the most frequent cause of diarrhoea in hospitals. Geriatric patients are more often affected by the condition, by a relapse and complications. Therefore, a crucial question is how often colonization with toxigenic Clostridium difficile strains occurs in elderly patients without diarrhoea and whether there is a "risk pattern" of colonized patients that can be defined by geriatric assessment. Furthermore, the probability for those asymptomatic carriers to develop a symptomatic infection over time has not been sufficiently explored...
November 15, 2016: BMC Geriatrics
https://www.readbyqxmd.com/read/27829823/a-new-lab-developed-real-time-pcr-assay-for-direct-detection-of-c-difficle-from-stool-sample-without-dna-extraction
#6
Brandon Li
Clostridium difficile is a major cause of nosocomial antibiotic-associated infectious diarrhea and pseudomembranous colitis. Detection of C. difficile by anaerobic bacterial culture and/or cytotoxicity assays has been largely replaced by rapid enzyme immunoassays (EIA). However, due to the lack of sensitivity of stool EIA, we developed a multiplex real-time PCR assay targeting the C. difficile toxin genes tcdB. stool samples from hospitalized pediatric patients suspected of having C. difficile-associated disease were prospectively collected...
September 2016: International Journal of Biomedical Science: IJBS
https://www.readbyqxmd.com/read/27825314/diagnostic-performance-of-smear-microscopy-and-incremental-yield-of-xpert-in-detection-of-pulmonary-tuberculosis-in-rwanda
#7
Jean Claude Semuto Ngabonziza, Willy Ssengooba, Florence Mutua, Gabriela Torrea, Augustin Dushime, Michel Gasana, Emmanuel Andre, Schifra Uwamungu, Alaine Umubyeyi Nyaruhirira, Dufton Mwaengo, Claude Mambo Muvunyi
BACKGROUND: Tuberculosis control program of Rwanda is currently phasing in light emitting diode-fluorescent microscopy (LED-FM) as an alternative to Ziehl-Neelsen (ZN) smear microscopy. This, alongside the newly introduced Xpert (Cepheid, Sunnyvale, CA, USA) is expected to improve diagnosis of tuberculosis and detection of rifampicin resistance in patients at health facilities. We assessed the accuracy of smear microscopy and the incremental sensitivity of Xpert at tuberculosis laboratories in Rwanda...
November 8, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27772674/geolocalization-of-influenza-outbreak-within%C3%A2-an%C3%A2-acute-care-population-a-layered-surveillance-approach
#8
Vijay Christopher Kannan, Nicole Hodgson, Andrew Lau, Kate Goodin, Andrea Freyer Dugas, Frank LoVecchio
STUDY OBJECTIVE: We seek to use a novel layered-surveillance approach to localize influenza clusters within an acute care population. The first layer of this system is a syndromic surveillance screen to guide rapid polymerase chain reaction testing. The second layer is geolocalization and cluster analysis of these patients. We posit that any identified clusters could represent at-risk populations who could serve as high-yield targets for preventive medical interventions. METHODS: This was a prospective observational surveillance study...
November 2016: Annals of Emergency Medicine
https://www.readbyqxmd.com/read/27737841/diagnostic-test-accuracy-in-childhood-pulmonary-tuberculosis-a-bayesian-latent-class-analysis
#9
Samuel G Schumacher, Maarten van Smeden, Nandini Dendukuri, Lawrence Joseph, Mark P Nicol, Madhukar Pai, Heather J Zar
Evaluation of tests for the diagnosis of childhood pulmonary tuberculosis (CPTB) is complicated by the absence of an accurate reference test. We present a Bayesian latent class analysis in which we evaluated the accuracy of 5 diagnostic tests for CPTB. We used data from a study of 749 hospitalized South African children suspected to have CPTB from 2009 to 2014. The following tests were used: mycobacterial culture, smear microscopy, Xpert MTB/RIF (Cepheid Inc.), tuberculin skin test (TST), and chest radiography...
November 1, 2016: American Journal of Epidemiology
https://www.readbyqxmd.com/read/27733636/point-of-care-cepheid-xpert-hiv-1-viral-load-test-in-rural-african-communities-is-feasible-and-reliable
#10
Sikhulile Moyo, Terence Mohammed, Kathleen E Wirth, Melanie Prague, Kara Bennett, Molly Pretorius Holme, Lucy Mupfumi, Philemon Sebogodi, Natasha O Moraka, Corretah Boleo, Comfort N Maphorisa, Boitumelo Seraise, Simani Gaseitsiwe, Rosemary M Musonda, Erik van Widenfelt, Kathleen M Powis, Tendani Gaolathe, Eric J Tchetgen Tchetgen, Joseph M Makhema, Max Essex, Shahin Lockman, Vladimir Novitsky
Routine monitoring of HIV-1 RNA or viral load (VL) in patients on antiretroviral therapy (ART) is important, but there are multiple impediments to VL testing in resource-constrained settings. An accurate point-of-care (POC) HIV-1 VL test could alleviate many of these challenges. We compared the performance of the Cepheid Xpert HIV-1 VL assay against the laboratory-based Abbott m2000sp/m2000rt assay (Abbott assay). ART-naive individuals participating in the Botswana Combination Prevention Project in 20 communities provided EDTA-blood specimens during household surveys...
December 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27625418/evaluation-of-the-performance-of-c-diff-quik-chek-complete-and-its-usefulness-in-a-hospital-setting-with-a-high-prevalence-of-clostridium-difficile-infection
#11
Hae-Sun Chung, Miae Lee
Rapid and accurate diagnosis of Clostridium difficile infection (CDI) is crucial for patient care, infection control, and efficient surveillance. We evaluated C. DIFF QUIK CHEK COMPLETE (QCC; TechLab), which detects glutamate dehydrogenase (GDH) antigen (QCC-Ag) and toxin A/B (QCC-Tox) simultaneously, and compared it to the laboratory diagnostics for CDI currently in use in a tertiary hospital setting with a high prevalence of CDI. QCC, RIDASCREEN C. difficile toxin A/B assay (Toxin EIA; R-Biopharm AG), chromID C...
September 13, 2016: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/27556286/performance-of-the-artus-c-difficile-qs-rgq-kit-for-the-detection-of-toxigenic-clostridium-difficile
#12
Hee-Won Moon, Hyeong Nyeon Kim, Ji-Young Kim, Mina Hur, Heejung Kim, Yeo-Min Yun
OBJECTIVES: Nucleic acid amplification tests are commonly used for the direct detection of toxigenic Clostridium difficile. We evaluated the diagnostic performance of newly launched, artus C. difficile QS-RGQ Kit (artus C. difficile, QIAGEN, Hilden, Germany), in comparison with toxigenic culture (TC) and Xpert C. difficile (Cepheid, Sunnyvale, CA, USA). DESIGN AND METHODS: In prospectively collected 261 diarrheal specimens, the artus C. difficile and the Xpert C...
August 21, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27552679/cepheid-xpert-monitor-platform-for-the-confirmation-of-bcr-abl1-is-conversion-factors-for-the-molecular-monitoring-of-chronic-myeloid-leukaemia
#13
Gareth Gerrard, Hui En Foong, Katherine Mudge, Mary Alikian, Jane F Apperley, Letizia Foroni
Molecular monitoring of BCR-ABL1 expression in chronic myeloid leukaemia (CML) is well established. As the International Scale (IS) normalised BCR-ABL1/ABL1 ratio at 3 months post-treatment is now an important milestone in patients' treatment schedule, the reliable and reproducible measurement of BCR-ABL1 levels is therefore paramount. IS conversion factors (CF) are established via sample exchange and yearly ratification with an external reference laboratory. Since any change to an established IS CF could lead to discontinuity in longitudinal results, we wished to add an internal verification step as an additional safeguard...
October 2016: Leukemia Research
https://www.readbyqxmd.com/read/27536736/validation-of-the-genexpert%C3%A2-ct-ng-assay-for-use-with-male-pharyngeal-and-rectal-swabs
#14
R Geiger, D M Smith, S J Little, S R Mehta
OBJECTIVES: The GeneXpert(®) CT/NG (Cepheid, Sunnyvale, CA) assay is a point-of-care (POC) molecular diagnostic assay designed to rapidly test for the presence of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC). However, the test is only approved for vaginal swabs, urine, and endocervical swabs. Here, we performed an evaluation of the GeneXpert(®) CT/NG assay to detect the presence of CT and GC on male pharyngeal and rectal swabs. METHODS: Men who have sex with men participating in an HIV and Sexually Transmitted Infection (STI) screening program providing consent were enrolled into the study...
2016: Austin Journal of HIV/AIDS Research
https://www.readbyqxmd.com/read/27532104/comparison-of-diagnostic-algorithms-for-detecting-toxigenic-clostridium-difficile-in-routine-practice-at-a-tertiary-referral-hospital-in-korea
#15
Hee-Won Moon, Hyeong Nyeon Kim, Mina Hur, Hee Sook Shim, Heejung Kim, Yeo-Min Yun
Since every single test has some limitations for detecting toxigenic Clostridium difficile, multistep algorithms are recommended. This study aimed to compare the current, representative diagnostic algorithms for detecting toxigenic C. difficile, using VIDAS C. difficile toxin A&B (toxin ELFA), VIDAS C. difficile GDH (GDH ELFA, bioMérieux, Marcy-l'Etoile, France), and Xpert C. difficile (Cepheid, Sunnyvale, California, USA). In 271 consecutive stool samples, toxigenic culture, toxin ELFA, GDH ELFA, and Xpert C...
2016: PloS One
https://www.readbyqxmd.com/read/27516471/universal-hospital-admission-screening-for-carbapenemase-producing-organisms-in-a-low-prevalence-setting
#16
Jonathan A Otter, Eleonora Dyakova, Karen N Bisnauthsing, Antonio Querol-Rubiera, Amita Patel, Chioma Ahanonu, Olga Tosas Auguet, Jonathan D Edgeworth, Simon D Goldenberg
BACKGROUND: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging threat for healthcare providers worldwide. OBJECTIVES: To determine CPE carriage rates and risk factors in an unselected hospital cohort at the time of admission. METHODS: We approached 4567 patients within 72 h of admission to provide a rectal swab and answer a questionnaire on risk factors for carriage. Rectal swabs were cultured for carbapenem-resistant organisms on chromogenic and non-chromogenic agar, and tested for carbapenemase production by PCR (Check-Direct CPE)...
August 11, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27510643/investigation-of-the-genexpert%C3%A2-ct-ng-assay-for-use-with-male-pharyngeal-and-rectal-swabs
#17
Rechel Geiger, David M Smith, Susan J Little, Sanjay R Mehta
The GeneXpert CT/NG (Cepheid, Sunnyvale, CA) is a point-of-care (POC) molecular diagnostic assay designed to detect Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC). However, the test is only approved for urine, vaginal, and endocervical swabs. Here, we evaluated the GeneXpert CT/NG assay on male pharyngeal and rectal swabs. Men-who-have-sex-with-men participating in a sexually transmitted infection (STI) screening program collected two sets of paired pharyngeal and rectal swabs. One set was tested using GeneXpert and the other using the APTIMA (Hologic, San Diego, CA, USA)...
August 10, 2016: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/27490684/use-of-cepheid-xpert-carba-r%C3%A2-for-rapid-detection-of-carbapenemase-producing-bacteria-in-abdominal-septic-patients-admitted-to-intensive-care-unit
#18
Andrea Cortegiani, Vincenzo Russotto, Giorgio Graziano, Daniela Geraci, Laura Saporito, Gianfranco Cocorullo, Santi Maurizio Raineri, Caterina Mammina, Antonino Giarratano
Early institution of effective antibiotic therapy and source control are pivotal to improve survival of abdominal septic patients. Xpert® Carba-R is a real time polymerase chain reaction assay for rapid detection and differentiation of five genes (blaKPC, blaVIM, blaOXA-48, blaIMP-1, blaNDM) responsible for carbapenem resistance. We performed an observational study investigating the clinical usefulness and applicability of Xpert® Carba-R to detect carbapenem resistance in abdominal septic patients admitted to intensive care unit...
2016: PloS One
https://www.readbyqxmd.com/read/27451855/rapid-and-accurate-detection-of-carbapenemase-genes-in-enterobacteriaceae-with-the-cepheid-xpert-carba-r-assay
#19
Marion Smith, Bram Diederen, Jelle Scharringa, Maurine Leversteijn-van Hall, Ad C Fluit, James Cohen Stuart
Rapid and accurate detection of carbapenemase-producing Enterobacteriaceae is pivotal for adequate antibiotic therapy and infection control. The Cepheid Xpert Carba-R assay detects and identifies the most prevalent carbapenemases (KPC, VIM, IMP, NDM and OXA-48), using automated real-time PCR. The test performance of the Xpert Carba-R was evaluated with 129 well-characterized non-repeat Enterobacteriaceae isolates, suspected for carbapenemase production, i.e. with meropenem MICs >0.25 mg l-1. The isolate collection contained 100 carbapenemase-producing isolates (36 KPC-2 or KPC-3, 20 VIM-1, 4 KPC-2 plus VIM-1, 5 NDM-1, 2 IMP-1, 1 IMP-28, 1 IMP-1 plus VIM-1 and 31 OXA-48 like) and 29 negative control isolates producing extended-spectrum β-lactamase and/or AmpC β-lactamases...
September 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27418550/development-roll-out-and-impact-of-xpert-mtb-rif-for-tuberculosis-what-lessons-have-we-learnt-and-how-can-we-do-better
#20
REVIEW
Heidi Albert, Ruvandhi R Nathavitharana, Chris Isaacs, Madhukar Pai, Claudia M Denkinger, Catharina C Boehme
The global roll-out of Xpert MTB/RIF (Cepheid Inc., Sunnyvale, CA, USA) has changed the diagnostic landscape of tuberculosis (TB). More than 16 million tests have been performed in 122 countries since 2011, and detection of multidrug-resistant TB has increased three- to eight-fold compared to conventional testing. The roll-out has galvanised stakeholders, from donors to civil society, and paved the way for universal drug susceptibility testing. It has attracted new product developers to TB, resulting in a robust molecular diagnostics pipeline...
August 2016: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
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