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https://www.readbyqxmd.com/read/29322584/effects-of-fixation-on-electrophysiology-and-structure-of-human-jejunal-villi
#1
Anna Casselbrant, Herbert F Helander
The villi of human jejunum vary in size and shape during different functional conditions. In the base the lamina propria is isotonic with blood, in the tip hyperosmotic. Here we study electrophysiological and morphological effects of incubation in hypotonic, isotonic, or hypertonic solutions, and to test various isotonic fixatives for microscopy. Samples of jejunal mucosae, obtained during surgery in obese patients, were studied in Ussing chambers where electrical parameters were registered during incubation in Krebs solution at various osmolarities, and during fixation in formaldehyde, glutaraldehyde, or osmium tetroxide (OsO4 )...
January 11, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29319761/isotonic-sports-drink-promotes-rehydration-and-decreases-proteinuria-following-karate-training
#2
Simone Geraldini, Igor de Freitas Cruz, Alexandre Romero, Fernando Luiz Affonso Fonseca, Michelle Parreira de Campos
INTRODUCTION: Adequate hydration status in the sport is essential for good health, yet the relationship between hydration, proteinuria and sports is little studied. OBJECTIVE: To analyze the influence of an isotonic sports drink as rehydration strategy on the hydration status and proteinuria after karate training. METHODS: Ten athletes participated in this study. In the first session of standard training, called observation training session (STO), the athletes hydrated themselves according to their habits, and in the second session of standard training, called nutritional intervention training session (STIN), an ideal practice of hydration protocol was followed, using an isotonic sports drink as a rehydration liquid during the training...
October 2017: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
https://www.readbyqxmd.com/read/29305835/vitrification-of-germinal-vesicle-stage-equine-oocytes-effect-of-cryoprotectant-exposure-time-on-in-vitro-embryo-production
#3
Heloísa Siqueira Canesin, Joao Gatto Brom-de-Luna, Young-Ho Choi, Amanda Macedo Pereira, Gustavo Guerino Macedo, Katrin Hinrichs
Previous studies have found low rates of blastocyst development (0-11%) after vitrification of germinal vesicle (GV)-stage equine oocytes. In this study, we systematically evaluated a short (non-equilibrating) system for GV-stage oocyte vitrification. In Exp. 1, we assessed oocyte volume in cumulus-oocyte complexes (COCs) exposed to components of a short protocol, using 2% each of ethylene glycol and propylene glycol in the first solution (VS1); 17.5% of each plus 0.3 M trehalose in the second solution (VS2); and fetal bovine serum as the base medium...
January 3, 2018: Cryobiology
https://www.readbyqxmd.com/read/29303137/comparison-of-icodextrin-with-thymoquinone-a-new-hope-for-postoperative-adhesions
#4
Ö Yilmaz, R Kiziltan, S Çelik, S Yildirm, H H Alp, A Aras, Ç Kotan
OBJECTIVE: The purpose of this study was to compare the effects of thymoquinone and icodextrin in rats within the framework of an experimental adhesion model. MATERIALS AND METHODS: Rats were separated into three groups: (1) a control group consisting of rats that had 2 ml of isotonic solution administered intraperitoneally, (2) an ICO group administered with 2 ml of 4% icodextrin, and (3) a TQ group administered thymoquinone (10 mg/kg), all following cecal abrasion...
November 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29298381/observation-of-dynamic-surfactant-adsorption-facilitated-by-divalent-cation-bridging
#5
Chase Gerold, Charles S Henry
Dynamic evidence of the mechanism for surfactant adsorption to surfaces of like charge has been observed. Additionally, removal and retention of surfactant molecules on the surface were observed as a function of time. A decrease in surface charge is observed when metal counter ions are introduced and is dependent on charge density as well as valency of the metal ion. When surfactant species are also present with the metals a dramatic increase in surface charge arises. We observed that the rate and quantity of surfactant adsorption can be controlled by the presence of divalent Ca2+...
January 3, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29290052/sialendoscopy-in-treatment-of-adult-chronic-recurrent-parotitis-without-sialolithiasis
#6
Johanna Jokela, Aaro Haapaniemi, Antti Mäkitie, Riitta Saarinen
OBJECTIVE: The aim of this prospective study was to evaluate the efficacy of sialendoscopy in the management of adult chronic recurrent parotitis without sialolithiasis. In addition, preliminary results of an initial randomized placebo-controlled trial of single-dose intraductal steroid injection given concurrently with sialendoscopy, are presented. METHODS: Forty-nine adult patients with chronic recurrent parotitis without sialoliths were included in this study...
December 30, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29288963/antioxidant-reserve-of-the-lungs-and-ventilator-associated-pneumonia-a-clinical-trial-of-high-dose-selenium-in-critically-ill-patients
#7
Ata Mahmoodpoor, Hadi Hamishehkar, Sarvin Sanaie, Nazila Behruzizad, Afshin Iranpour, Evin Koleini, Nader D Nader
PURPOSE: To evaluate the effect of supplemental selenium administration on the incidence of ventilator-associated pneumonia (VAP) in critically ill patients. METHODS: Ninety-nine mechanically ventilated patients were randomized to receive either selenium or isotonic saline infusion for 10days. The primary endpoint was serum glutathione peroxidase-3 (GPX-3) activity and secondary endpoints were development of VAP or death, ICU stay and vasopressor requirement. Serum concentrations of selenium and GPX-3 were measured on Day-1, Day-4 and Day-10...
December 24, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/29286433/methodology-for-sputum-induction-and-laboratory-processing
#8
Julien Guiot, Sophie Demarche, Monique Henket, Virginie Paulus, Sophie Graff, Florence Schleich, Jean-Louis Corhay, Renaud Louis, Catherine Moermans
The technique of sputum induction and processing is a recognized non-invasive method allowing the collection and analysis of cells from the airways, which is interesting in various respiratory diseases like asthma, chronic obstructive pulmonary disease (COPD), chronic cough, or idiopathic pulmonary fibrosis. This technique is well tolerated, safe and non-invasive, but is currently limited to research services and specialized centers in clinical practice because it is technically demanding, time-consuming, and requires trained staff...
December 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29283924/comparison-of-changes-in-central-corneal-thickness-during-corneal-collagen-cross-linking-using-isotonic-riboflavin-solutions-with-and-without-dextran-in-the-treatment-of-progressive-keratoconus
#9
Naima Zaheer, Wajid Ali Khan, Shama Khan, M Abdul Moqeet Khan
PURPOSE: To compare intraoperative changes in central corneal thickness (CCT) during corneal cross-linking, using 2 different isotonic riboflavin solutions either with dextran or with hydroxy propyl methylcellulose, in the treatment of progressive keratoconus. METHODS: In this retrospective study, we analyzed records of corneal thickness measurements, taken during various steps of cross-linking. Cross-linking was performed using either isotonic riboflavin with dextran (group A) or isotonic riboflavin with hydroxy propyl methylcellulose (without dextran) (group B)...
December 27, 2017: Cornea
https://www.readbyqxmd.com/read/29279574/sports-drink-consumption-and-dental-erosion-among-amateur-runners
#10
Leonardo S Antunes, Lais Veiga, Victor S Nery, Caio C Nery, Lívia A Antunes
This cross-sectional study assessed the prevalence and potential risk factors for dental erosion in amateur athletes at running events. After a sample calculation, 108 runners from the state of Rio de Janeiro, Brazil, were selected and examined for dental wear by a single trained and calibrated evaluator (kappa = 1.00). To identify risk factors, the runners were interviewed by using a standardized, semi-structured questionnaire. The average (SD) age of the runners was 34.2 (11.45), and the prevalence of dental erosion was 19...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29273902/ampk-dependent-nitric-oxide-release-provides-contractile-support-during-hyperosmotic-stress
#11
Malena Morell, Juan Ignacio Burgos, Luis Alberto Gonano, Martin Vila Petroff
In different pathological situations, cardiac cells undergo hyperosmotic stress (HS) and cell shrinkage. This change in cellular volume has been associated with contractile dysfunction and cell death. Given that nitric oxide (NO) is a well-recognized modulator of cardiac contractility and cell survival, we evaluated whether HS increases NO production and its impact on the negative inotropic effect observed during this type of stress. Superfusing cardiac myocytes with a hypertonic solution (HS: 440 mOsm) decreased cell volume and increased NO-sensitive DAF-FM fluorescence compared with myocytes superfused with an isotonic solution (IS: 309 mOsm)...
December 22, 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/29272915/shape-dependent-dissolution-and-cellular-uptake-of-silver-nanoparticles
#12
Christina Maria Graf, Daniel Nordmeyer, Christina Sengstock, Sebastian Ahlberg, Joerg Diendorf, Jörg Raabe, Matthias Epple, Manfred Köller, Juergen Lademann, Annika Vogt, Fiorenza Rancan, Eckart Ruehl
Cellular uptake and dissolution of trigonal silver nanoprisms (edge length: 42±15 nm, thickness: 8±1 nm) and mostly spherical silver nanoparticles (diameter: 70±25 nm) in human mesenchymal stem cells (hMSC) and human keratinocytes (HaCaT cells) were investigated. Both particles are stabilized by polyvinylpyrrolidone (PVP), the prisms additionally by citrate. The nanoprisms dissolved slightly in pure water but strongly in isotonic saline or at pH 4, corresponding to the lowest limit for the pH during cellular uptake...
December 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29248941/evaluation-of-an-experimental-remineralizing-agent-for-repairing-enamel-surfaces
#13
Margarita V Úsuga Vacca, Carolina Torres-Rodríguez, Edgar Delgado-Mejía
The best material for repairing enamel surface defects is one very similar to the original enamel and which interacts with natural remineralization mechanisms. It does not repair extensive damage, so in order to fill large defects, external help is required using phosphocalcic ceramic composites that activate salivary remineralization efficiently though on smaller in scale. Effective adhesion of the repair may depend on the amount of aqueous fluids present in the enamel, which apparently enable nucleation and growth of new minerals to ensure adhesion and stability...
August 2017: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/29248141/impact-of-volume-status-and-volume-therapy-on-the-kidney
#14
REVIEW
David A Roberts, Andrew D Shaw
Volume resuscitation to correct hypotension in surgical and critically ill patients is a common practice. Available evidence suggests that iatrogenic volume overload is associated with worse outcomes in established acute kidney injury. Intraoperative arterial hypotension is associated with postoperative renal dysfunction, and prompt correction with fluid management protocols that combine inotrope infusions with volume therapy targeted to indices of volume responsiveness should be considered. From the perspective of renal function, the minimum amount of intravenous fluid required to maintain perfusion and oxygen delivery is desirable...
September 2017: Best Practice & Research. Clinical Anaesthesiology
https://www.readbyqxmd.com/read/29244973/cooperativity-between-antibiotics-and-antiseptics-testing-the-bactericidal-effect
#15
S Jenull, H Laggner, I Hassl, B Velimirov, M Huettinger, N Zemann
OBJECTIVE: Treatment with antibiotics together with local application of antiseptics is common in wound care. We investigated the effectiveness of an antiseptic in two variations: octenidine (Oct) and octenidine+ (Oct+ with isotonic glucose addition). METHOD: Using the agar diffusion test with cultures of pathogenic Staphylococcus aureus and the non-pathogenic Bordetella petrii, we compared the effectiveness of octenidine to the classical antiseptics beta-isodona (povidone-iodine; PI), chlorhexidine (Chl) and taurolin (Tau) alone, and in combination with various common antibiotics to uncover cooperativity between antiseptics and antibiotics...
December 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/29244891/the-effects-of-experimental-temporalis-muscle-pain-on-jaw-muscle-electromyographic-activity-during-jaw-movements-and-relationships-with-some-psychological-variables
#16
Tantry Maulina, Magda Amhamed, Terry Whittle, John Gal, Rahena Akhter, Greg M Murray
AIMS: To determine if the effects of experimental temporalis muscle pain on jaw muscle activity vary with the jaw task performed, jaw displacement magnitude, participant being studied, and with psychological measures. METHODS: Jaw movement was tracked, and electromyographic (EMG) activity was recorded from the masseter and anterior temporalis and digastric muscles in 14 asymptomatic participants during standardized opening/closing jaw movement, free chewing, and standardized chewing tasks...
December 15, 2017: Journal of Oral & Facial Pain and Headache
https://www.readbyqxmd.com/read/29238597/effects-of-nine-weeks-isokinetic-training-on-power-golf-kinematics-and-driver-performance-in-pre-elite-golfers
#17
James Parker, Charlie Lagerhem, John Hellström, M Charlotte Olsson
Background: It has previously been shown that isotonic strength training can improve driver performance among golfers, though few studies have investigated effects of strength training on swing kinematics together with driver performance. In this study we investigated whether isokinetic rotational training could improve driver performance and swing kinematic variables amongst elite golfers. Methods: Twenty competitive pre-elite golfers (handicap better than -3.0), 13 men and 7 women, were split into two groups, one group received the isokinetic power training (IK) alongside their normal isotonic pre-season strength-training and the other group continued with their normal isotonic pre-season strength-training regime (IT)...
2017: BMC Sports Science, Medicine and Rehabilitation
https://www.readbyqxmd.com/read/29238262/effect-of-upper-extremity-strengthening-exercises-on-the-lumbar-strength-disability-and-pain-of-patients-with-chronic-low-back-pain-a-randomized-controlled-study
#18
Erdem Atalay, Bedrettin Akova, Hakan Gür, Ufuk Sekir
The present study aimed to analyze the impacts of a low back rehabilitation program accompanied with neck, shoulder and upper back exercises on pain, disability, and physical characteristics of patients with chronic low back pain. Twenty sedentary male patients with chronic low back pain participated in the study on a voluntary basis. The patients were randomly allocated into two groups: a conventional low back exercise group (CE) and a supported exercise group (SE; CE plus upper back, neck, and shoulder exercises)...
December 2017: Journal of Sports Science & Medicine
https://www.readbyqxmd.com/read/29238166/cardiovascular-liver-and-renal-toxicity-associated-with-an-intravenous-ferric-carboxymaltose-similar-versus-the-originator-compound
#19
Jorge E Toblli, Gabriel Cao, Luis Rico, Margarita Angerosa
Background: Ferric carboxymaltose (FCM) is a stable, non-dextran-based intravenous iron complex used to treat iron deficiency of various etiologies. As FCM is a nonbiological complex drug and cannot be fully characterized by physicochemical analyses, it is important to demonstrate in nonclinical models that FCM similars (FCMS) have similar biodistribution. Materials and methods: A total of 30 nonanemic rats were treated weekly with 40 mg iron/kg body weight intravenous FCM, FCMS, or isotonic saline (controls) for 4 weeks...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29232903/pediatric-thermal-burns-and-treatment-a-review-of-progress-and-future-prospects
#20
REVIEW
Elton Mathias, Madhu Srinivas Murthy
Burn injuries are a devastating critical care problem. In children, burns continue to be a major epidemiologic problem around the globe resulting in significant morbidity and death. Apparently, treating these burn injuries in children and adults remains similar, but there are significant physiological and psychological differences. The dermal layer of the skin is generally thinner in neonates, infants, and children than in adults. Enhanced evaporative loss and need for isotonic fluids increases the risk of hypothermia in the pediatric population...
December 11, 2017: Medicines (Basel, Switzerland)
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