keyword
MENU ▼
Read by QxMD icon Read
search

Neutrophil imaging

keyword
https://www.readbyqxmd.com/read/28634671/venous-thromboembolism-is-an-independent-predictor-of-mortality-among-patients-with-gastric-cancer
#1
Harry E Fuentes, D M Oramas, L H Paz, Y Wang, X A Andrade, A J Tafur
BACKGROUND: Venous thromboembolism (VTE) is an independent predictor of death among patients with cancer. Patients with gastric cancer (GC) are at higher risk for VTE when compared to other solid tumors, and if one considers its prevalence, GC may be responsible for one of the highest incidences of cancer-associated thrombosis. The impact of VTE on mortality is not well defined among patients with GC. AIM: The aim of this study is to measure the impact of VTE as independent predictor of GC mortality...
June 21, 2017: Journal of Gastrointestinal Cancer
https://www.readbyqxmd.com/read/28624043/composition-of-the-cellular-infiltrate-in-patients-with-simple-and-complex-appendicitis
#2
Ramon R Gorter, Emma C E Wassenaar, Onno J de Boer, Roel Bakx, Joris J T H Roelofs, Madeleine J Bunders, L W Ernst van Heurn, Hugo A Heij
BACKGROUND: It is now well established that there are two types of appendicitis: simple (nonperforating) and complex (perforating). This study evaluates differences in the composition of the immune cellular infiltrate in children with simple and complex appendicitis. MATERIALS AND METHODS: A total of 47 consecutive children undergoing appendectomy for acute appendicitis between January 2011 and December 2012 were included. Intraoperative criteria were used to identify patients with either simple or complex appendicitis and were confirmed histopathologically...
June 15, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28606749/cardiovascular-disease-in-systemic-lupus-erythematosus-a-comprehensive-update
#3
REVIEW
Mayra Giannelou, Clio P Mavragani
Heightened rates of both cardiovascular (CV) events and subclinical atherosclerosis, documented by imaging and vascular function techniques are well established in systemic lupus erythematosus (SLE). While traditional CV factors such as smoking, dyslipidemia, diabetes mellitus (DM), hypertension, central obesity and hyperhomocysteinemia have been reported to be prevalent in lupus patients, they do not fully explain the high rates of ischemic events so far reported, implying that other factors inherent to disease itself could account for the enhanced risk, including disease duration, activity and chronicity, psychosocial factors, medications, genetic variants and altered immunological mechanisms...
June 9, 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/28601571/intrasellar-dermoid-cyst-mimicking-pituitary-apoplexy-a-case-report-and-review-of-the-literature
#4
Yuan-Bo Pan, Zhao-Liang Sun, Dong-Fu Feng
Intrasellar dermoid cysts are extremely unusual lesions, with only four cases reported to date, and have not been previously reported in association with sudden-onset symptoms. Here, we present the case of an intrasellar dermoid cyst with sudden-onset symptoms mimicking pituitary apoplexy in an elderly woman. A 69year-old woman presented with sudden onset of headache, dizziness, and decreased visual acuity. Magnetic resonance imaging of the sellar region showed an intrasellar lesion, which showed mixed hyper- and hypointense signal on T1-weighted and T2-weighted images and enhanced peripherally...
June 7, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28601234/comparison-of-echocardiographic-findings-in-patients-with-nonfunctioning-adrenal-incidentalomas
#5
Narin Nasıroglu Imga, Ozgul Ucar Elalmıs, Mazhar Muslum Tuna, Bercem Aycıcek Dogan, Deniz Sahın, Tugba Gursoy, Yavuz Yalcın, Dilek Berker, Serdar Guler
Adrenal incidentalomas (AIs) are usually discovered incidentally after imaging unrelated to adrenal glands. We aimed to evaluate standard risk factors for systemic atherosclerosis and echocardiographic changes in patients with nonfunctioning AIs and compare them with normal subjects. We evaluated 70 patients diagnosed with AIs and 51 healthy controls. Mean levels were determined for HbA1c, LDL, uric acid, fasting plasma insulin, HOMA, and neutrophil-to-lymphocyte ratio (NLR), and these values were found to be significantly higher in the patients than the controls...
June 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/28598511/evaluating-atlantic-bottlenose-dolphin-tursiops-truncatus-leukocyte-differential-counts-comparing-the-cellavision-dm96-and-the-manual-method
#6
Julia Zaias, Christine Bradley, Jill Richardson, Holli C Eskelinen, Frances Ikpatt, Carolyn Cray
BACKGROUND: The leukocyte differential count is an excellent diagnostic tool; however, the manual differential count has several drawbacks, especially for nontraditional species. Automated cell analyzers commonly used in veterinary practices require species-specific validation for use in nondomestic species other than dogs and cats. OBJECTIVES: The purpose of this study was to examine the potential of the CellaVision DM96 (DM96), an automated image analysis system, as a rapid and accurate method for providing a WBC differential count in comparison to the manual WBC differential count in bottlenose dolphins...
June 9, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28592427/positron-emission-tomography-imaging-of-macaques-with-tuberculosis-identifies-temporal-changes-in-granuloma-glucose-metabolism-and-integrin-%C3%AE-4%C3%AE-1-expressing-immune-cells
#7
Joshua T Mattila, Wissam Beaino, Pauline Maiello, M Teresa Coleman, Alexander G White, Charles A Scanga, JoAnne L Flynn, Carolyn J Anderson
Positron emission tomography and computed tomography imaging (PET/CT) is an increasingly valuable tool for diagnosing tuberculosis (TB). The glucose analog [(18)F]fluoro-2-deoxy-2-d-glucose ([(18)F]-FDG) is commonly used in PET/CT that is retained by metabolically active inflammatory cells in granulomas, but lacks specificity for particular cell types. A PET probe that could identify recruitment and differentiation of different cell populations in granulomas would be a useful research tool and could improve TB diagnosis and treatment...
June 7, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28588217/a-haptotaxis-assay-for-neutrophils-using-optical-patterning-and-a-high-content-approach
#8
Joannie Roy, Javier Mazzaferri, János G Filep, Santiago Costantino
Neutrophil recruitment guided by chemotactic cues is a central event in host defense against infection and tissue injury. While the mechanisms underlying neutrophil chemotaxis have been extensively studied, these are just recently being addressed by using high-content approaches or surface-bound chemotactic gradients (haptotaxis) in vitro. Here, we report a haptotaxis assay, based on the classic under-agarose assay, which combines an optical patterning technique to generate surface-bound formyl peptide gradients as well as an automated imaging and analysis of a large number of migration trajectories...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28584077/vascular-sprouts-induce-local-attraction-of-proangiogenic-neutrophils
#9
Gustaf Christoffersson, Jalal Lomei, Paul O'Callaghan, Johan Kreuger, Stefan Engblom, Mia Phillipson
Angiogenesis, the growth of new blood vessels, is a complex process requiring the orchestration of numerous different cell types, growth factors, and chemokines. Some of the recently acknowledged actors in this process are immune cells. They accumulate at hypoxic sites, but the kinetics, dynamics, and regulation of that trafficking are unknown. In this study, we used intravital and live cell imaging to understand how neutrophils and macrophages migrate and behave at angiogenic sites. We developed two reproducible models of angiogenesis: one by transplanting isolated and hypoxic pancreatic islets into the cremaster muscles of mice, and another by in vitro coculturing of mouse aortic rings with neutrophils...
June 5, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28579520/live-imaging-of-the-innate-immune-response-in-neonates-reveals-differential-tlr2-dependent-activation-patterns-in-sterile-inflammation-and-infection
#10
Melanie Lalancette-Hébert, Joel Faustino, Sai Sampath Thammisetty, Sophorn Chip, Zinaida S Vexler, Jasna Kriz
Activation of microglial cells in response to brain injury and/or immune stimuli is associated with a marked induction of Toll-like receptors (TLRs). While in adult brain, the contribution of individual TLRs, including TLR2, in pathophysiological cascades has been well established, their role and spatial and temporal induction patterns in immature brain are far less understood. To examine whether infectious stimuli and sterile inflammatory stimuli trigger distinct TLR2-mediated innate immune responses, we used three models in postnatal day 9 (P9) mice, a model of infection induced by systemic endotoxin injection and two models of sterile inflammation, intra-cortical IL-1β injection and transient middle cerebral artery occlusion (tMCAO)...
June 1, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28559109/real-time-visualization-of-immune-cell-clearance-of-aspergillus-fumigatus-spores-and-hyphae
#11
Benjamin P Knox, Anna Huttenlocher, Nancy P Keller
Invasive aspergillosis (IA) is a disease of the immunocompromised host and generally caused by the opportunistic fungal pathogen Aspergillus fumigatus. While both host and fungal factors contribute to disease severity and outcome, there are fundamental features of IA development including fungal morphological transition from infectious conidia to tissue-penetrating hyphae as well as host defenses rooted in mechanisms of innate phagocyte function. Here we address recent advances in the field and use real-time in vivo imaging in the larval zebrafish to visually highlight conserved vertebrate innate immune behaviors including macrophage phagocytosis of conidia and neutrophil responses post-germination...
May 27, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28554852/g%C3%AE-1-is-required-for-neutrophil-migration-in-zebrafish
#12
Wenfan Ke, Ding Ye, Kacey Mersch, Hui Xu, Songhai Chen, Fang Lin
Signaling mediated by G protein-coupled receptors (GPCRs) is essential for the migration of cells toward chemoattractants. The recruitment of neutrophils to injured tissues in zebrafish larvae is a useful model for studying neutrophil migration and trafficking in vivo. Indeed, the study of this process led to the discovery that PI3Kγ is required for the polarity and motility of neutrophils, features that are necessary for the directed migration of these cells to wounds. However, the mechanism by which PI3Kγ is activated remains to be determined...
May 27, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28547129/quantitative-analysis-of-dynamic-18-f-fdg-pet-ct-for-measurement-of-lung-inflammation
#13
Christopher Coello, Marie Fisk, Divya Mohan, Frederick J Wilson, Andrew P Brown, Michael I Polkey, Ian Wilkinson, Ruth Tal-Singer, Philip S Murphy, Joseph Cheriyan, Roger N Gunn
BACKGROUND: An inflammatory reaction in the airways and lung parenchyma, comprised mainly of neutrophils and alveolar macrophages, is present in some patients with chronic obstructive pulmonary disease (COPD). Thoracic fluorodeoxyglucose ((18)F-FDG) positron emission tomography (PET) has been proposed as a promising imaging biomarker to assess this inflammation. We sought to introduce a fully quantitative analysis method and compare this with previously published studies based on the Patlak approach using a dataset comprising (18)F-FDG PET scans from COPD subjects with elevated circulating inflammatory markers (fibrinogen) and matched healthy volunteers (HV)...
December 2017: EJNMMI Research
https://www.readbyqxmd.com/read/28544679/sima-a-simplified-migration-assay-for-analyzing-neutrophil-migration
#14
Markus Weckmann, Tim Becker, Gyde Nissen, Martin Pech, Matthias V Kopp
In lung inflammation, neutrophils are the first leukocytes migrating to an inflammatory site, eliminating pathogens by multiple mechanisms. The term "migration" describes several stages of neutrophil movement to reach the site of inflammation, of which the passage of the interstitium and basal membrane of the airway are necessary to reach the site of bronchial inflammation. Currently, several methods exist (e.g., Boyden Chamber, under-agarose assay, or microfluidic systems) to assess neutrophil mobility. However, these methods do not allow for parameterization on single cell level, that is, the individual neutrophil pathway analysis is still considered challenging...
May 19, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28542336/does-ngal-reduce-costs-a-cost-analysis-of-urine-ngal-ungal-serum-creatinine-scr-for-acute-kidney-injury-aki-diagnosis
#15
Amay Parikh, John A Rizzo, Pietro Canetta, Catherine Forster, Meghan Sise, Omar Maarouf, Eugenia Singer, Antje Elger, Saban Elitok, Kai Schmidt-Ott, Jonathon Barasch, Thomas L Nickolas
INTRODUCTION: Urine neutrophil gelatinase-associated lipocalin (uNGAL) is a sensitive and specific diagnostic test for acute kidney injury (AKI) in the Emergency Department (ED), but its economic impact has not been investigated. We hypothesized that uNGAL used in combination with serum creatinine (sCr) would reduce costs in the management of AKI in patients presenting to the ED in comparison to using sCr alone. MATERIALS AND METHODS: A cost simulation model was developed for clinical algorithms to diagnose AKI based on sCr alone vs...
2017: PloS One
https://www.readbyqxmd.com/read/28535994/diabetic-choroidopathy
#16
Gerard A Lutty
Early histopathological studies of diabetic choroids demonstrated loss of choriocapillaris (CC), tortuous blood vessels, microaneurysms, drusenoid deposits on Bruchs membrane, and choroidal neovascularization. The preponderance of histopathological changes were at and beyond equator. Studies from my lab suggest that diabetic choroidopathy is an inflammatory disease in that leukocyte adhesion molecules are elevated in the choroidal vasculature and polymorphonuclear neutrophils are often associated with sites of vascular loss...
May 20, 2017: Vision Research
https://www.readbyqxmd.com/read/28529998/complement-c5a-receptor-is-the-key-initiator-of-neutrophil-adhesion-igniting-immune-complex-induced-arthritis
#17
Yoshishige Miyabe, Chie Miyabe, Thomas T Murooka, Edward Y Kim, Gail A Newton, Nancy D Kim, Bodduluri Haribabu, Francis W Luscinskas, Thorsten R Mempel, Andrew D Luster
The deposition of immune complexes (IC) in tissues induces a "type III hypersensitivity" that results in tissue damage and underlies the pathogenesis of many autoimmune diseases. The neutrophil is the first immune cell recruited into sites of IC deposition and plays a critical role in shaping the overall tissue response. However, the mechanism by which IC initiate and propagate neutrophil infiltration into tissue is not known. Here, using intravital multiphoton joint imaging of IC-induced arthritis in live mice, we found that the complement C5a receptor (C5aR) was the key initiator of neutrophil adhesion on joint endothelium...
January 2017: Science Immunology
https://www.readbyqxmd.com/read/28528454/new-insights-into-pericarditis-mechanisms-of-injury-and-therapeutic-targets
#18
REVIEW
Bo Xu, Serge C Harb, Paul C Cremer
PURPOSE OF REVIEW: This review article aims to provide a contemporary insight into the pathophysiological mechanisms of and therapeutic targets for pericarditis, drawing distinction between autoinflammatory and autoimmune pericarditis. RECENT FINDINGS: Recent research has focused on the distinction between autoinflammatory and autoimmune pericarditis. In autoinflammatory pericarditis, viruses can activate the sensor molecule of the inflammasome, which results in downstream release of cytokines, such as interleukin-1, that recruit neutrophils and macrophages to the site of injury...
July 2017: Current Cardiology Reports
https://www.readbyqxmd.com/read/28526762/exacerbation-of-thromboinflammation-by-hyperglycemia-precipitates-cerebral-infarct-growth-and-hemorrhagic-transformation
#19
Jean-Philippe Desilles, Varouna Syvannarath, Véronique Ollivier, Clément Journé, Sandrine Delbosc, Célina Ducroux, William Boisseau, Liliane Louedec, Lucas Di Meglio, Stéphane Loyau, Martine Jandrot-Perrus, Louis Potier, Jean-Baptiste Michel, Mikael Mazighi, Benoit Ho-Tin-Noé
BACKGROUND AND PURPOSE: Admission hyperglycemia is associated with a poor outcome in acute ischemic stroke. How hyperglycemia impacts the pathophysiology of acute ischemic stroke remains largely unknown. We investigated how preexisting hyperglycemia increases ischemia/reperfusion cerebral injury. METHODS: Normoglycemic and streptozotocin-treated hyperglycemic rats were subjected to transient middle cerebral artery occlusion. Infarct growth and brain perfusion were assessed by magnetic resonance imaging...
May 19, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28489916/generational-distribution-of-a-candida-glabrata-population-resilient-old-cells-prevail-while-younger-cells-dominate-in-the-vulnerable-host
#20
Tejas Bouklas, Luz Alonso-Crisóstomo, Tamás Székely, Elizabeth Diago-Navarro, Erika P Orner, Kalie Smith, Mansa A Munshi, Maurizio Del Poeta, Gábor Balázsi, Bettina C Fries
Similar to other yeasts, the human pathogen Candida glabrata ages when it undergoes asymmetric, finite cell divisions, which determines its replicative lifespan. We sought to investigate if and how aging changes resilience of C. glabrata populations in the host environment. Our data demonstrate that old C. glabrata are more resistant to hydrogen peroxide and neutrophil killing, whereas young cells adhere better to epithelial cell layers. Consequently, virulence of old compared to younger C. glabrata cells is enhanced in the Galleria mellonella infection model...
May 2017: PLoS Pathogens
keyword
keyword
53542
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"