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Neutrophil imaging

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https://www.readbyqxmd.com/read/29327941/infection-is-not-required-for-mucoinflammatory-lung-disease-in-cftr-knockout-ferrets
#1
Bradley H Rosen, T Idil Apak Evans, Shashanna R Moll, Jaimie S Gray, Bo Liang, Xingshen Sun, Yulong Zhang, Chandler W Jensen-Cody, Anthony M Swatek, Weihong Zhou, Nan He, Pavana G Rotti, Scott R Tyler, Nicholas W Keiser, Preston J Anderson, Leonard Brooks, Yalan Li, R Marshall Pope, Maheen Rajput, Eric A Hoffman, Kai Wang, J Kirk Harris, Kalpaj R Parekh, Katherine N Gibson-Corley, John F Engelhardt
RATIONALE: Classical interpretation of cystic fibrosis (CF) lung disease pathogenesis suggests that infection initiates disease progression, leading to an exuberant inflammatory response, excessive mucus, and ultimately bronchiectasis. While symptomatic antibiotic treatment controls lung infections early in disease, life-long bacterial residence typically ensues. Processes that control the establishment of persistent bacteria in the CF lung, and the contribution of non-infectious components to disease pathogenesis, are poorly understood...
January 12, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29324635/diagnostic-value-of-serum-urokinase-type-plasminogen-activator-receptor-in-children-with-acute-appendicitis
#2
Mustafa Onur Oztan, Aysegul Aksoy Gokmen, Fatma Demet Arslan, Ebru Cakir, Ali Sayan, Elif Abay, Selçuk Kaya, Gokhan Koyluoglu
OBJECTIVES: Acute appendicitis (AA) is the most common surgical emergency in children. The accurate and timely diagnosis of AA in children can be challenging, and delayed diagnosis rates have been reported to range from 5.9% to 27.6%. Although combining clinical history and repeated physical examination with laboratory tests and radiographic imaging modalities help reach the diagnosis, novel biomarkers can support the surgeons' decision as well. The aims of this study were to evaluate a new plasma marker, urokinase-type plasminogen activator receptor (uPAR), to improve diagnostic accuracy in AA patients, and to determine a cutoff value of uPAR, which can safely include/exclude the diagnosis of AA...
January 11, 2018: Pediatric Emergency Care
https://www.readbyqxmd.com/read/29311408/hepatic-hydrothorax
#3
Yong Lv, Guohong Han, Daiming Fan
Hepatic hydrothorax (HH) is a pleural effusion that develops in a patient with cirrhosis and portal hypertension in the absence of cardiopulmonary disease. Although the development of HH remains incompletely understood, the most acceptable explanation is that the pleural effusion is a result of a direct passage of ascitic fluid into the pleural cavity through a defect in the diaphragm due to the raised abdominal pressure and the negative pressure within the pleural space. Patients with HH can be asymptomatic or present with pulmonary symptoms such as shortness of breath, cough, hypoxemia, or respiratory failure associated with large pleural effusions...
December 27, 2017: Annals of Hepatology
https://www.readbyqxmd.com/read/29302254/terminally-differentiating-eosinophils-express-neutrophil-primary-granule-proteins-as-well-as-eosinophil-specific-granule-proteins-in-a-temporal-manner
#4
Karam Kim, Sae Mi Hwang, Sung Min Kim, Sung Woo Park, Yunjae Jung, Il Yup Chung
Neutrophils and eosinophils, 2 prominent granulocytes, are commonly derived from myelocytic progenitors through successive stages in the bone marrow. Our previous genome-wide transcriptomic data unexpectedly showed that genes encoding a multitude of neutrophil primary granule proteins (NPGPs) were markedly downregulated during the end period of eosinophilic terminal differentiation when cord blood (CB) cluster of differentiation (CD) 34+ cells were induced to differentiate toward the eosinophil lineage during a 24-day culture period...
December 2017: Immune Network
https://www.readbyqxmd.com/read/29299363/does-histology-really-influence-gastric-cancer-prognosis
#5
Carrie Luu, Ram Thapa, Katherine Woo, Domenico Coppola, Khaldoun Almhanna, Jose M Pimiento, Dung-Tsa Chen, Daissy Dominguez Marquez, Pamela J Hodul
Background: Gastric cancer (GC) is associated with poor survival despite curative-intent surgical resection and systemic therapy. Our objective is to examine the impact of histology on prognosis as well as the impact of linitis plastica (LP) on survival. Methods: The GC database at a single institution was evaluated for patients who underwent resection from 2000 to 2015. Clinicopathologic characteristics were examined and descriptive statistics was used to analyze four groups of patients based on Lauren classification: intestinal (n=93), diffuse (n=20), diffuse with signet-ring cell features (n=57), and LP (n=40)...
December 2017: Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/29290892/differential-diagnoses-of-magnetic-resonance-imaging-for-suspected-acute-appendicitis-in-pregnant-patients
#6
Ji Yong Jung, Ji Ung Na, Sang Kuk Han, Pil Cho Choi, Jang Hee Lee, Dong Hyuk Shin
BACKGROUND: Accurate and timely diagnosis of acute surgical disease in pregnant patient is challenging. Although magnetic resonance imaging (MRI) is the most accurate modality to diagnose acute appendicitis in pregnant patients, it is often used as a last resort because of high cost and long scan time. We performed this study to analyze differential diagnoses of appendix MRI and to investigate if there are any blood tests that can predict surgical condition in pregnant patients. METHODS: A retrospective, cross-sectional study was conducted on 46 pregnant patients who underwent non-enhanced appendix MRI in suspicion of acute appendicitis from 2010 to 2016...
2018: World Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29290790/changes-in-the-microarchitecture-of-the-pancreatic-cancer-stroma-are-linked-to-neutrophil-dependent-reprogramming-of-stellate-cells-and-reflected-by-diffusion-weighted-magnetic-resonance-imaging
#7
Philipp Mayer, Christine Dinkic, Ralf Jesenofsky, Miriam Klauss, Peter Schirmacher, Ulrike Dapunt, Thilo Hackert, Florian Uhle, G Maria Hänsch, Matthias M Gaida
In pancreatic cancer (PDAC) intratumor infiltration of polymorphonuclear neutrophils (PMN) is associated with histologically apparent alterations of the tumor growth pattern. The aim of this study was to examine possible associations between PMN infiltration, tumor microarchitecture, and water diffusivity in diffusion-weighted magnetic resonance imaging (DW-MRI), and to further asses the underlying mechanisms. Methods: DW-MRI was performed in 33 PDAC patients prior to surgery. In parallel, tissue specimen were examined histologically for growth pattern, azurocidin-positive PMN infiltrates, and the presence of alpha-smooth muscle actin (α-SMA) and metalloproteinase 9 (MMP9)-positive myofibroblastic cells...
2018: Theranostics
https://www.readbyqxmd.com/read/29288079/17-18-epete-gpr40-axis-ameliorates-contact-hypersensitivity-by-inhibiting-neutrophil-mobility-in-mice-and-cynomolgus-macaques
#8
Takahiro Nagatake, Yumiko Shiogama, Asuka Inoue, Junichi Kikuta, Tetsuya Honda, Prabha Tiwari, Takayuki Kishi, Atsushi Yanagisawa, Yosuke Isobe, Naomi Matsumoto, Michiko Shimojou, Sakiko Morimoto, Hidehiko Suzuki, Soichiro Hirata, Pär Steneberg, Helena Edlund, Junken Aoki, Makoto Arita, Hiroshi Kiyono, Yasuhiro Yasutomi, Masaru Ishii, Kenji Kabashima, Jun Kunisawa
BACKGROUND: Metabolites of eicosapentaenoic acid (EPA) exert various physiological actions. 17,18-epoxyeicosatetraenoic acid (17,18-EpETE) is a recently identified new class of anti-allergic and anti-inflammatory lipid metabolite of EPA, but its effects on skin inflammation and the underlying mechanisms remain to be investigated. OBJECTIVE: We evaluated the effectiveness of 17,18-EpETE for the control of contact hypersensitivity in mouse and cynomolgus macaques...
December 26, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29287721/intravital-imaging-of-neutrophil-recruitment-in-intestinal-ischemia-reperfusion-injury
#9
Shintaro Hashimoto, Masaki Honda, Takayuki Takeichi, Masataka Sakisaka, Yasuko Narita, Daiki Yoshii, Keiichi Uto, Seisuke Sakamoto, Yukihiro Inomata
BACKGROUND: Neutrophils are known to be key players in innate immunity. Activated neutrophils induce local inflammation, which results in pathophysiologic changes during intestinal ischemia-reperfusion injury (IRI). However, most studies have been based on static assessments, and few have examined real-time intravital neutrophil recruitment. We herein report a method for imaging and evaluating dynamic changes in the neutrophil recruitment in intestinal IRI using two-photon laser scanning microscopy (TPLSM)...
January 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29287105/effect-of-intra-articular-administration-of-superparamagnetic-iron-oxide-nanoparticles-spions-for-mri-assessment-of-the-cartilage-barrier-in-a-large-animal-model
#10
Raphael Labens, Carola Daniel, Sarah Hall, Xin-Rui Xia, Tobias Schwarz
Early diagnosis of cartilage disease at a time when changes are limited to depletion of extracellular matrix components represents an important diagnostic target to reduce patient morbidity. This report is to present proof of concept for nanoparticle dependent cartilage barrier imaging in a large animal model including the use of clinical magnetic resonance imaging (MRI). Conditioned (following matrix depletion) and unconditioned porcine metacarpophalangeal cartilage was evaluated on the basis of fluorophore conjugated 30 nm and 80 nm spherical gold nanoparticle permeation and multiphoton laser scanning and bright field microscopy after autometallographic particle enhancement...
2017: PloS One
https://www.readbyqxmd.com/read/29286634/high-throughput-screening-raman-spectroscopy-hts-rs-platform-for-label-free-cellomics
#11
Iwan W Schie, Jan Rüger, Abdullah Saif Mondol, Anuradha Ramoji, Ute Neugebauer, Christoph Krafft, Juergen Popp
We present a high-throughput screening Raman spectroscopy (HTS-RS) platform for a rapid and label-free macromolecular fingerprinting of tens of thousands eukaryotic cells. The newly proposed label-free HTS-RS platform combines automated imaging microscopy with Raman spectroscopy to enable a rapid label-free screening of cells and can be applied to a large number of biomedical and clinical applications. The potential of the new approach is illustrated by two applications: 1. HTS-RS-based differential white blood cell count...
December 29, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29285884/two-year-observational-study-of-deferiprone-in-superficial-siderosis
#12
Remi A Kessler, Xu Li, Kateryna Schwartz, Hwa Huang, Maureen A Mealy, Michael Levy
INTRODUCTION: Superficial siderosis is a rare, neurodegenerative disease caused by toxic accumulation of hemosiderin on the surface of the brain and the spinal cord, most commonly from chronic subarachnoid hemorrhage. AIMS: The aim of this study was to assess the clinical and radiological outcomes of superficial siderosis patients using deferiprone, a cell permeant iron chelator. Subjects obtained pre- and post-treatment brain MRIs and weekly laboratory tests. Osirix software was used to develop a method of quantifying hemosiderin deposition...
December 28, 2017: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/29282442/impact-of-cyberknife-radiosurgery-on-overall-survival-and-various-parameters-of-patients-with-1-3-versus-%C3%A2-4-brain-metastases
#13
Judith Murovic, Victoria Ding, Summer S Han, John R Adler, Steven D Chang
Introduction This study's objective is to compare the overall survivals (OSs) and various parameters of patients with 1-3 versus ≥ 4 brain metastases post-CyberKnife radiosurgery (CKRS) (Accuray, Sunnyvale, California) alone. Methods Charts of 150 patients, from 2009-2014, treated with only CKRS for brain metastases were reviewed retrospectively for overall survival (OS) and patient, tumor, and imaging characteristics. Parameters included demographics, Eastern Cooperative Oncology Group (ECOG) performance scores, number and control of extracranial disease (ECD) sites, cause of death (COD), histology, tumor volume (TV), and post-CKRS whole brain radiotherapy (WBRT)...
October 24, 2017: Curēus
https://www.readbyqxmd.com/read/29279515/anti-neutrophil-cytoplasmic-antibody-associated-vasculitis-aav-restricted-to-the-limbs-a-case-report
#14
Yoshie Ojima, Kinya Sawada, Hiroshi Fujii, Tsuyoshi Shirai, Ayako Saito, Saeko Kagaya, Satoshi Aoki, Yoichi Takeuchi, Tomonori Ishii, Tasuku Nagasawa
A previously healthy 58-year-old man was admitted for muscle pain and weakness (manual muscle testing [MMT] of 4/4 for upper and lower limbs). We detected elevated levels of inflammatory makers and PR3-anti-neutrophil cytoplasmic antibody (ANCA). Subsequently, the muscle weakness rapidly progressed to an MMT of 2 for all limbs. Magnetic resonance imaging indicated muscle edema, and the CK level increased to 29,998 U/L. mPSL and cyclophosphamide pulse therapy improved the patient symptoms. MMT recovered to 4 for all limbs...
December 27, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29275396/variant-of-lemierre-s-syndrome-with-internal-jugular-vein-aneurysm
#15
Siew Houy Chua, Sidney Ching Liang Ong, Yuan Hwen Liew
Internal jugular vein (IJV) aneurysm is a rare entity, and a thrombosed aneurysm poses diagnostic and management challenges. We came across a 53-year-old woman who presented with fever, vomiting and right neck swelling for a week. Laboratory investigations showed neutrophilic leucocytosis, raised acute phase reactant and blood culture yielded Klebsiella pneumoniae Ultrasound and contrast-enhanced CT neck revealed a large fusiform aneurysm of the right IJV with filling defect extending from the aneurysm into the right transverse sinus...
December 22, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29273298/recruitment-and-therapeutic-application-of-macrophages-in-skeletal-muscles-after-hind-limb-ischemia
#16
Pei-Ling Hsieh, Viktoriya Rybalko, Aaron B Baker, Laura J Suggs, Roger P Farrar
OBJECTIVE: Peripheral arterial disease can cause not only ischemia but also skeletal muscle damage. It has been known that macrophages (MPs) play an important role in coordinating muscle repair; however, phenotype transition of monocyte-MP in ischemic muscle has not been well defined. Hence, the purpose of this study was to examine the temporal recruitment of MPs and to explore their therapeutic effect on ischemic muscle regeneration. METHODS: Unilateral femoral artery excision was performed on C57BL/6 mice...
December 19, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29273069/single-walled-carbon-nanotubes-modulate-pulmonary-immune-responses-and-increase-pandemic-influenza-a-virus-titers-in-mice
#17
Hao Chen, Xiao Zheng, Justine Nicholas, Sara T Humes, Julia C Loeb, Sarah E Robinson, Joseph H Bisesi, Dipesh Das, Navid B Saleh, William L Castleman, John A Lednicky, Tara Sabo-Attwood
BACKGROUND: Numerous toxicological studies have focused on injury caused by exposure to single types of nanoparticles, but few have investigated how such exposures impact a host's immune response to pathogen challenge. Few studies have shown that nanoparticles can alter a host's response to pathogens (chiefly bacteria) but there is even less knowledge of the impact of such particles on viral infections. In this study, we performed experiments to investigate if exposure of mice to single-walled carbon nanotubes (SWCNT) alters immune mechanisms and viral titers following subsequent influenza A virus (IAV) infection...
December 22, 2017: Virology Journal
https://www.readbyqxmd.com/read/29261466/case-249-intramuscular-mycetoma
#18
Zafar Neyaz, Namita Mohindra, Ankur Bhatnagar, Rungmei S K Marak
History A 21-year-old man presented with swelling of the medial aspect of the left thigh of 1-month duration. There was no history of fever or penetrating injury in the left thigh. The patient had undergone renal transplantation 7 years earlier and had been taking immunosuppressants since transplantation. He had undergone two surgeries at the same site in the medial aspect of the left thigh in the past 3 years for a similar problem. At physical examination, there was swelling in the medial aspect of the left thigh, with mild tenderness...
January 2018: Radiology
https://www.readbyqxmd.com/read/29259241/computational-detection-and-quantification-of-human-and-mouse-neutrophil-extracellular-traps-in-flow-cytometry-and-confocal-microscopy
#19
Brandon G Ginley, Tiffany Emmons, Brendon Lutnick, Constantin F Urban, Brahm H Segal, Pinaki Sarder
Neutrophil extracellular traps (NETs) are extracellular defense mechanisms used by neutrophils, where chromatin is expelled together with histones and granular/cytoplasmic proteins. They have become an immunology hotspot, implicated in infections, but also in a diverse array of diseases such as systemic lupus erythematosus, diabetes, and cancer. However, the precise assessment of in vivo relevance in different disease settings has been hampered by limited tools to quantify occurrence of extracellular traps in experimental models and human samples...
December 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29237455/molecular-and-histological-effects-of-mr-guided-pulsed-focused-ultrasound-to-the-rat-heart
#20
Kee W Jang, Tsang-Wei Tu, Matthew E Nagle, Bobbi K Lewis, Scott R Burks, Joseph A Frank
BACKGROUND: Image-guided high intensity focused ultrasound has been used as an extracorporeal cardiac pacing tool and to enhance homing of stem cells to targeted tissues. However, molecular changes in the myocardium after sonication have not been widely investigated. Magnetic-resonance (MR)-guided pulsed focused ultrasound (pFUS) was targeted to the rat myocardium over a range of pressures and the microenvironmental and histological effects were evaluated over time. METHODS: Eight-to-ten-week-old Sprague-Dawley rats received T2-weighted MR images to target pFUS to the left ventricular and septum without cardiac or respiratory gating...
December 13, 2017: Journal of Translational Medicine
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