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Neutrophil imaging

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https://www.readbyqxmd.com/read/28087377/in-vivo-biodistribution-and-imaging-studies-with-a-99m-tc-radiolabeled-derivative-of-the-c-terminus-of-prothymosin-alpha-in-mice-bearing-experimentally-induced-inflammation
#1
Chrysoula-Evangelia Karachaliou, Charalampos Triantis, Christos Liolios, Lazaros Palamaris, Christos Zikos, Ourania E Tsitsilonis, Hubert Kalbacher, Wolfgang Voelter, George Loudos, Minas Papadopoulos, Ioannis Pirmettis, Evangelia Livaniou
Prothymosin alpha (ProTα) is a highly conserved mammalian polypeptide (109 amino acids in man) exerting in vitro and in vivo immunoenhancing activities. Recently, our team has developed a (99m)Tc-radiolabeled derivative of the C-terminal bioactive decapeptide of ProTα ([(99m)Tc]C1) and employed it in in vitro studies, the results of which support the existence of binding sites on human neutrophils that recognize [(99m)Tc]C1, intact ProTα as well as the C-terminal decapeptide of ProTα and presumably involve Toll-like receptor 4...
January 11, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28079960/successful-medical-management-of-a-domestic-longhair-cat-with-subdural-intracranial-empyema-and-multifocal-pneumonia
#2
Thomas J A Cardy, Richard Lam, Laureen M Peters, Philippa J McLaren, Màrian Matas Riera, Steven De Decker
OBJECTIVE: To describe a case of successful medical management of subdural intracranial empyema and multifocal pneumonia in a domestic longhaired cat. CASE SUMMARY: A 7-year-and-8-month-old male neutered domestic longhair cat presented with tachypnea, respiratory compromise, vestibular ataxia, obtundation, left-sided head tilt, and multiple cranial nerve deficits. Neuroanatomical localization was multifocal with central vestibular involvement. Magnetic resonance imaging of the head indicated diffuse subdural empyema, mainly affecting the middle cranial fossa and the right cerebrum...
January 12, 2017: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/28073784/platelets-and-neutrophil-extracellular-traps-collaborate-to-promote-intravascular-coagulation-during-sepsis-in-mice
#3
Braedon McDonald, Rachelle P Davis, Seok-Joo Kim, Mandy Tse, Charles T Esmon, Elzbieta Kolaczkowska, Craig N Jenne
Neutrophil extracellular traps (NETs; webs of DNA coated in anti-microbial proteins) are released into the vasculature during sepsis where they contribute to host defense, but also cause tissue damage and organ dysfunction. Various components of NETs have also been implicated as activators of coagulation. Using multi-color confocal intravital microscopy in mouse models of sepsis, we observed profound platelet aggregation, thrombin activation, and fibrin clot formation within (and downstream of) NETs in vivo...
January 10, 2017: Blood
https://www.readbyqxmd.com/read/28062701/widespread-virus-replication-in-alveoli-drives-acute-respiratory-distress-syndrome-in-aerosolized-h5n1-influenza-infection-of-macaques
#4
Elizabeth R Wonderlich, Zachary D Swan, Stephanie J Bissel, Amy L Hartman, Jonathan P Carney, Katherine J O'Malley, Adebimpe O Obadan, Jefferson Santos, Reagan Walker, Timothy J Sturgeon, Lonnie J Frye, Pauline Maiello, Charles A Scanga, Jennifer D Bowling, Anthea L Bouwer, Parichat A Duangkhae, Clayton A Wiley, JoAnne L Flynn, Jieru Wang, Kelly S Cole, Daniel R Perez, Douglas S Reed, Simon M Barratt-Boyes
Human infections with highly pathogenic avian influenza A (H5N1) virus are frequently fatal but the mechanisms of disease remain ill-defined. H5N1 infection is associated with intense production of proinflammatory cytokines, but whether this cytokine storm is the main cause of fatality or is a consequence of extensive virus replication that itself drives disease remains controversial. Conventional intratracheal inoculation of a liquid suspension of H5N1 influenza virus in nonhuman primates likely results in efficient clearance of virus within the upper respiratory tract and rarely produces severe disease...
January 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28062700/intravital-imaging-of-neutrophil-recruitment-reveals-the-efficacy-of-fpr1-blockade-in-hepatic-ischemia-reperfusion-injury
#5
Masaki Honda, Takayuki Takeichi, Shintaro Hashimoto, Daiki Yoshii, Kaori Isono, Shintaro Hayashida, Yuki Ohya, Hidekazu Yamamoto, Yasuhiko Sugawara, Yukihiro Inomata
Neutrophils are considered responsible for the pathophysiological changes resulting from hepatic ischemia-reperfusion (I/R) injury, which is a complication of trauma, shock, liver resection, and transplantation. Recently, evidence is accumulating that formyl-peptide receptor (FPR) signaling constitutes an important danger signal that guides neutrophils to sites of inflammation. This study aimed to investigate dynamic neutrophil recruitment using two-photon laser-scanning microscopy (TPLSM) in response to FPR1 blockade during hepatic I/R...
January 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28062502/targeting-elastase-for-molecular-imaging-of-early-atherosclerotic-lesions
#6
Almut Glinzer, Xiaopeng Ma, Jaya Prakash, Melanie A Kimm, Fabian Lohöfer, Katja Kosanke, Jaroslav Pelisek, Moritz P Thon, Sandra Vorlova, Katrin G Heinze, Hans-Henning Eckstein, Michael W Gee, Vasilis Nitziachristos, Alma Zernecke, Moritz Wildgruber
OBJECTIVE: Neutrophils accumulate in early atherosclerotic lesions and promote lesion growth. In this study, we evaluated an elastase-specific near-infrared imaging agent for molecular imaging using hybrid fluorescence molecular tomography/x-ray computed tomography. APPROACH AND RESULTS: Murine neutrophils were isolated from bone marrow and incubated with the neutrophil-targeted near-infrared imaging agent Neutrophil Elastase 680 FAST for proof of principle experiments, verifying that the elastase-targeted fluorescent agent is specifically cleaved and activated by neutrophil content after lysis or cell stimulation...
December 29, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28059587/metabolic-effects-of-fasting-on-human-and-mouse-blood-in-vivo
#7
Federico Pietrocola, Yohann Demont, Francesca Castoldi, David Enot, Sylvère Durand, Michaela Semeraro, Elisa Elena Baracco, Jonathan Pol, Jose Manuel Bravo-San Pedro, Chloé Bordenave, Sarah Levesque, Juliette Humeau, Alexis Chery, Didier Métivier, Frank Madeo, M Chiara Maiuri, Guido Kroemer
Starvation is a strong physiological stimulus of macroautophagy/autophagy. In this study, we addressed the question as to whether it would be possible to measure autophagy in blood cells after nutrient deprivation. Fasting of mice for 48 h (which causes ∼20% weight loss) or starvation of human volunteers for up to 4 days (which causes <2% weight loss) provokes major changes in the plasma metabolome, yet induces only relatively minor alterations in the intracellular metabolome of circulating leukocytes...
January 6, 2017: Autophagy
https://www.readbyqxmd.com/read/28050750/a-novel-modality-for-functional-imaging-in-acute-intervertebral-disk-herniation-via-tracking-leukocyte-infiltration
#8
Li Xiao, Mengmeng Ding, Yi Zhang, Mahendra Chordia, Dongfeng Pan, Adam Shimer, Francis Shen, David Glover, Li Jin, Xudong Li
PURPOSE: Inflammation plays a key role in the progression of intervertebral disk (IVD) herniation and associated low back pain. However, real-time spatial diagnosis of inflammation associated with acute disk herniation has not been investigated. We sought to detect local neutrophil and macrophage infiltration near disk herniation via the formyl peptide receptor 1 (FPR1)-mediated molecular imaging in a disk puncture mouse model to elucidate pathophysiological process of disk herniation...
January 3, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28045905/human-leukocytes-kill-brugia-malayi-microfilariae-independently-of-dna-based-extracellular-trap-release
#9
Ciaran J McCoy, Barbara J Reaves, Steeve Giguère, Ruby Coates, Balázs Rada, Adrian J Wolstenholme
BACKGROUND: Wuchereria bancrofti, Brugia malayi and Brugia timori infect over 100 million people worldwide and are the causative agents of lymphatic filariasis. Some parasite carriers are amicrofilaremic whilst others facilitate mosquito-based disease transmission through blood-circulating microfilariae (Mf). Recent findings, obtained largely from animal model systems, suggest that polymorphonuclear leukocytes (PMNs) contribute to parasitic nematode-directed type 2 immune responses. When exposed to certain pathogens PMNs release extracellular traps (NETs) in the form of chromatin loaded with various antimicrobial molecules and proteases...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28042058/eosinophilic-airway-inflammation-in-asthma-is-associated-with-an-altered-airway-microbiome
#10
Asger Sverrild, Pia Kiilerich, Asker Brejnrod, Rebecca Pedersen, Celeste Porsbjerg, Anders Bergqvist, Jonas S Erjefält, Karsten Kristiansen, Vibeke Backer
BACKGROUND: Subjects with asthma have higher microbiome diversity, and an altered composition with more Proteobacteria and less Bacteroidetes compared to healthy controls. Studies comparing airway inflammation and airway microbiome are sparse, especially in subjects not on anti-inflammatory treatment. OBJECTIVE: To describe the relationship between the airway microbiome and patterns of airway inflammation in steroid-free subjects with asthma and healthy controls...
December 29, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28035206/neutrophil-extravasation-cascade-what-can-we-learn-from-two-photon-intravital-imaging
#11
REVIEW
Sang A Park, Young-Min Hyun
Immune cells (leukocytes or white blood cells) move actively and sensitively based on body conditions. Despite their important role as protectors inside the body, it is difficult to directly observe the spatiotemporal momentum of leukocytes. With advances in imaging technology, the introduction of two-photon microscopy has enabled researchers to look deeper inside tissues in a three-dimensional manner. In observations of immune cell movement along the blood vessel, vascular permeability and innate immune cell movements remain unclear...
December 2016: Immune Network
https://www.readbyqxmd.com/read/28029433/mmp-1-and-mmp-8-expression-in-giant-cell-fibroma-and-inflammatory-fibrous-hyperplasia
#12
Henrique Climeck de Oliveira, André Tschoeke, Gabriele Claudino da Cruz, Lúcia Noronha, Rafaela Scariot de Moraes, Ricardo Alves Mesquita, Maria Cássia Ferreira de Aguiar, Patrícia Carlos Caldeira, Marina de Oliveira Ribas, Ana Maria Trindade Grégio, Luciana Reis Azevedo Alanis, Sérgio Aparecido Ignácio, Jean Nunes Dos Santos, Antonio Adilson Soares de Lima, Aline Cristina Batista Rodrigues Johann
The aim of this study is to compare the immunoexpression of metalloproteinases 1 and 8 in giant-cell fibroma, inflammatory fibrous hyperplasia and normal mucosa. Twenty-two cases of giant-cell fibroma, inflammatory fibrous hyperplasia and oral mucosa (control) each were subjected to immunohistochemistry using anti-metalloproteinase-1 and anti-metalloproteinase-8 antibodies. Eight images of each case were captured and analysed through the a) application of a count grid to count the number of positive neutrophils, macrophages, lymphocytes, plasma cells, fibroblasts and blood vessels to obtain the percentage of staining and b) semi-automated segmentation quantifying the stained area in square micrometres...
October 15, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28027808/-splenic-rupture-secondary-to-abscess-rare-cause-of-pneumoperitoneum-case-report
#13
Jorge Enrique Barrón-Reyes, Julio César Chávez-Galván, Jesús Alejandro Martínez-Peralta, Julio César López-Valdés
BACKGROUND: Splenic abscesses are rare entities; reports are commonly described in immunocompromised patients (72%) as: hematologic diseases, diabetes, endocarditis, acquired immunodeficiency syndrome, transplant patients and subjects who had abdominal trauma or splenic infarction. The main and most serious complication is the abscess rupture into the peritoneal cavity or adjacent organs (stomach or colon), which determines hemodynamic instability or septic state. CLINICAL CASE: Fifty-year-old man, who was admitted at Emergency Room due eight days' progressive, oppressive, and current pain; intensity 4/10, irradiated at hemi-back, which was higher intensity during the standing and decreased at supine position...
December 24, 2016: Cirugia y Cirujanos
https://www.readbyqxmd.com/read/28011495/granulocyte-colony-stimulating-factor-producing-anaplastic-carcinoma-of-the-pancreas-case-report-and-review-of-the-literature
#14
Sarah Vinzens, Joel Zindel, Martin Zweifel, Tilman Rau, Beat Gloor, Annette Wochner
We report on the case of a 67-year-old man with granulocyte colony-stimulating factor (G-CSF) producing anaplastic carcinoma of the pancreas. Preoperative routine tests revealed an elevated white blood cell (WBC) count of 25.2 G/l, consisting almost exclusively of neutrophilic granulocytes (23.31 G/l) with a predominance of segmented neutrophils (78% of all neutrophilic granulocytes), and elevated levels of C-reactive protein at 87 mg/l. Upon surgery, local tumour infiltration was more extensive than expected from preoperative imaging...
January 2017: Anticancer Research
https://www.readbyqxmd.com/read/28003814/the-selective-estrogen-receptor-modulator-raloxifene-inhibits-neutrophil-extracellular-trap-formation
#15
Roxana Flores, Simon Döhrmann, Christina Schaal, Abdul Hakkim, Victor Nizet, Ross Corriden
Raloxifene is a selective estrogen receptor modulator typically prescribed for the prevention/treatment of osteoporosis in postmenopausal women. Although raloxifene is known to have anti-inflammatory properties, its effects on human neutrophils, the primary phagocytic leukocytes of the immune system, remain poorly understood. Here, through a screen of pharmacologically active small molecules, we find that raloxifene prevents neutrophil cell death in response to the classical activator phorbol 12-myristate 13-acetate (PMA), a compound known to induce formation of DNA-based neutrophil extracellular traps (NETs)...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27998729/in%C3%A2-vivo-imaging-of-inflamed-glomeruli-reveals-dynamics-of-neutrophil-extracellular-trap-formation-in-glomerular-capillaries
#16
Clare L V Westhorpe, James E Bayard, Kim M O'Sullivan, Pam Hall, Qiang Cheng, A Richard Kitching, Michael J Hickey
Neutrophil extracellular traps (NETs) have been documented in glomeruli of patients with glomerulonephritis. However, the dynamics of NET formation in the glomerulus and their functional contribution to acute glomerular injury are poorly understood. Herein, we used in vivo multiphoton microscopy to investigate NET formation in the acutely inflamed glomerulus. Glomerular inflammation was induced using an antibody against the glomerular basement membrane. After induction of glomerular inflammation, multiphoton microscopy revealed that approximately 20% of glomeruli contained structures composed of extracellular DNA within the capillaries...
December 17, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27997925/immunothrombosis-in-acute-respiratory-distress-syndrome-cross-talks-between-inflammation-and-coagulation
#17
Frantzeska Frantzeskaki, Apostolos Armaganidis, Stylianos E Orfanos
Acute respiratory distress syndrome (ARDS) is defined as a syndrome of acute onset, with bilateral opacities on chest imaging and respiratory failure not caused by cardiac failure, leading to mild, moderate, or severe oxygenation impairment. The syndrome is most commonly a manifestation of sepsis-induced organ dysfunction, characterized by disruption of endothelial barrier integrity and diffuse lung damage. Imbalance between coagulation and inflammation is a predominant characteristic of ARDS, leading to extreme inflammatory response and diffuse fibrin deposition in vascular capillary bed and alveoli...
December 21, 2016: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/27994782/non-caseating-granulomatous-infective-spondylitis-melioidotic-spondylitis
#18
Justin Arockiaraj, Rajiv Karthik, Veena Jeyaraj, Rohit Amritanand, Venkatesh Krishnan, Kenny Samuel David, Gabriel David Sundararaj
STUDY DESIGN: Retrospective clinical analysis. PURPOSE: To delineate the clinical presentation of melioidosis in the spine and to create awareness among healthcare professionals, particularly spine surgeons, regarding the diagnosis and treatment of melioidotic spondylitis. OVERVIEW OF LITERATURE: Melioidosis is an emerging disease, particularly in developing countries, associated with a high mortality rate. Its causative pathogen, Burkholderia pseudomallei, has been labeled as a bio-terrorism agent...
December 2016: Asian Spine Journal
https://www.readbyqxmd.com/read/27993028/nanocontact-printing-of-proteins-on-physiologically-soft-substrates-to-study-cell-haptotaxis
#19
Donald MacNearney, Bernard Mak, Grant Ongo, Timothy E Kennedy, David Juncker
Surface bound guidance cues and gradients are vital for directing cellular processes during development and repair. In vivo, these cues are often presented within a soft extracellular matrix with elastic moduli E < 10 kPa, but in vitro haptotaxis experiments have been conducted primarily on hard substrates with elastic moduli in the MPa to GPa range. Here, a technique is presented for patterning haptotactic proteins with nanometer resolution on soft substrates with physiological elasticity. A new nanocontact printing process was developed that circumvented the use of plasma activation that was found to alter the mechanical properties of the substrate...
December 20, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27981484/uric-acid-is-protective-after-cerebral-ischemia-reperfusion-in-hyperglycemic-mice
#20
Carles Justicia, Angélica Salas-Perdomo, Isabel Pérez-de-Puig, Lisette H Deddens, Geralda A F van Tilborg, Clara Castellví, Rick M Dijkhuizen, Ángel Chamorro, Anna M Planas
Hyperglycemia at stroke onset is associated with poor long-term clinical outcome in numerous studies. Hyperglycemia induces intracellular acidosis, lipid peroxidation, and peroxynitrite production resulting in the generation of oxidative and nitrosative stress in the ischemic tissue. Here, we studied the effects of acute hyperglycemia on in vivo intercellular adhesion molecule-1 (ICAM-1) expression, neutrophil recruitment, and brain damage after ischemia/reperfusion in mice and tested whether the natural antioxidant uric acid was protective...
December 15, 2016: Translational Stroke Research
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