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Haiyin Li, Jiafu Chang, Panpan Gai, Feng Li
Fluorescence biosensing strategy has drawn substantial attention due to their advantages of simplicity, convenience, sensitivity and selectivity, but unsatisfactory structure stability, low fluorescence quantum yield, high cost of labeling, and strict reaction conditions associated with current fluorescence methods severely prohibit their potential application. To address these challenges, we herein propose an ultrasensitive label-free fluorescence biosensor by integrating hemin/G-quadruplex-catalyzed oxidation reaction with aggregation induced emission (AIE) fluorogen-based system...
January 16, 2018: ACS Applied Materials & Interfaces
Ruibo Zhong, Mingshu Xiao, Changfeng Zhu, Xizhong Shen, Qian Tang, Weijia Zhang, Lihua Wang, Shiping Song, Xiangmeng Qu, Hao Pei, Cheng Wang, Li Li
By incorporating hemin into G-quadruplex (G4) during cation-templated self-assembly between guanosine and KB(OH)4, we have constructed an artificial enzyme hydrogel (AEH)-based system for the highly sensitive and selective detection of Pb2+. The sensing strategy is based on a Pb2+-induced decrease in AEH activity. Due to the higher efficiency of Pb2+ for stabilizing G4 compared with K+, the Pb2+ ions substitute K+ and trigger hemin release from G4, thus giving rise to a conformational interconversion accompanied by the loss of enzyme activity...
January 16, 2018: ACS Applied Materials & Interfaces
Mingchun Zhang, Zhizhong Zhang, Zhong Li, Mingsheng Hong, Xiaoping Zhou, Shiqiang Zhou, Jindong Zhang, Vanessa Hull, Jinyan Huang, Hemin Zhang
Diet plays a pivotal role in dictating behavioral patterns of herbivorous animals, particularly specialist species. The giant panda (Ailuropoda melanoleuca) is well-known as a bamboo specialist. In the present study, the response of giant pandas to spatiotemporal variation of bamboo shoots was explored using field surveys and GPS collar tracking. Results show the dynamics in panda-bamboo space-time relationships that have not been previously articulated. For instance, we found a higher bamboo stump height of foraged bamboo with increasing elevation, places where pandas foraged later in spring when bamboo shoots become more fibrous and woody...
January 10, 2018: Environmental Science and Pollution Research International
Tingting Hou, Yuanfu Zhang, Tao Wu, Meifeng Wang, Yinghong Zhang, Rui Li, Lei Wang, Qingwang Xue, Shuhao Wang
A simple, label-free colorimetric method for the determination of fibrinogen (Fib) in plasma is presented. In this work, it was observed that Fib interacted with hemin to form a hemin-Fib composite. Because Fib prevented hemin from the formation of m-oxo-dimers, the hemin-Fib composite possesses excellent peroxidase-like activity. Importantly, the peroxidase-like activity of Fib-hemin increased with the increase in the Fib. This allows us to utilize the H2O2-ABTS colorimetric system for the quantitative analysis of Fib...
January 11, 2018: Analyst
Shinichi Sato, Michihiko Tsushima, Kosuke Nakamura, Hiroyuki Nakamura
 The chemical labeling of proteins with synthetic probes is a key technique used in chemical biology, protein-based therapy, and material science. Much of the chemical labeling of native proteins, however, depends on the labeling of lysine and cysteine residues. While those methods have significantly contributed to native protein labeling, alternative methods that can modify different amino acid residues are still required. Herein we report the development of a novel methodology of tyrosine labeling, inspired by the luminol chemiluminescence reaction...
2018: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
Lindsey R Lyman, Eric D Peng, Michael P Schmitt
Corynebacterium diphtheriae utilizes various heme-containing proteins, including hemoglobin (Hb) and the hemoglobin-haptoglobin complex (Hb-Hp), as iron sources during growth in iron-depleted environments. The ability to utilize Hb-Hp as an iron source requires the surface anchored proteins HtaA and either ChtA or ChtC. The ability to bind hemin, Hb and Hb-Hp by each of these C. diphtheriae proteins requires the previously characterized Conserved Region (CR) domain. In this study, we identified an Hb-Hp binding protein, HbpA (38...
January 8, 2018: Journal of Bacteriology
Meng-I Lin, Takashi Nagata, Masato Katahira
Oxidative enzymes of white-rot fungi play a key role in lignin biodegradation. Among those fungus, Ceriporiopsis subvermispora degrades lignin before cellulose in wood; C. subvermispora is the only fungus that secretes all known types of manganese peroxidases (CsMnPs). Utilization of lignin-degrading peroxidases has been limited so far due to the lack of efficient preparation methods and intensive characterization. In this study, we developed a highly efficient method to prepare active CsMnPs through soluble expression by E...
January 2, 2018: Protein Expression and Purification
Seong U Kim, Bhagwan S Batule, Hyoyoung Mun, Ju-Young Byun, Won-Bo Shim, Min-Gon Kim
We have developed a novel strategy for the colorimetric detection of PCR products by utilizing a target-specific primer modified at the 5'-end with an anti-DNAzyme sequence. A single-stranded DNAzyme sequence folds into a G-quadruplex structure with hemin and shows strong peroxidase activity. When the complementary strand binds to the DNAzyme sequence, it blocks the formation of the G-quadraduplex structure and loses its peroxidase activity. In the presence of the target gene, PCR amplification proceeds, and anti-DNAzyme sequence modified primers present in the reaction mixture form a double strand through primer extension...
January 4, 2018: Analyst
Peng Guo, Bo Zhu, Hong Niu, Zezhao Wang, Yonghu Liang, Yan Chen, Lupei Zhang, Hemin Ni, Yong Guo, El Hamidi A Hay, Xue Gao, Huijiang Gao, Xiaolin Wu, Lingyang Xu, Junya Li
BACKGROUND: Running multiple-chain Markov Chain Monte Carlo (MCMC) provides an efficient parallel computing method for complex Bayesian models, although the efficiency of the approach critically depends on the length of the non-parallelizable burn-in period, for which all simulated data are discarded. In practice, this burn-in period is set arbitrarily and often leads to the performance of far more iterations than required. In addition, the accuracy of genomic predictions does not improve after the MCMC reaches equilibrium...
January 3, 2018: BMC Bioinformatics
Caitlin Burrell, Hemin Zhang, Desheng Li, Chengdong Wang, Caiwu Li, Copper Aitken-Palmer
The giant panda ( Ailuropoda melanoleuca) is a high-profile threatened species with individuals in captivity worldwide. As a result of advances in captive animal management and veterinary medicine, the ex situ giant panda population is aging, and improved understanding of age-related changes is necessary. Urine and blood samples were collected in April and July 2015 and analyzed for complete blood count, serum biochemistry, and biochemical and microscopic urine analysis for all individuals sampled ( n = 7, 7-16 yr of age) from giant panda housed at the China Research and Conservation Centre for the Giant Panda in Bifengxia, Sichuan Province, China...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Shirin Attarian, Chunli Yu, Karl E Anderson, Ellen W Friedman
Hemin and hemodialysis had an additive effect in decreasing ALA and PBG in our patient with acute intermittent porphyria and renal failure.The time course of ALA and PBG reaccumulation after hemodialysis is not known.
June 13, 2017: Blood Advances
Xiangguo Wang, Boyang Zhu, Shanhui Xiong, Xihui Sheng, Xiaolong Qi, Qizhen Huang, Chaolei Chen, Yong Guo, Hemin Ni
Mucin 1 (MUC1), a cell surface glycoprotein, is expressed mainly in the endometrial luminal epithelium (LE) and glandular epithelium (GE) of the endometrium in many mammalian species including mice, rats, pigs, sheep, horses and humans during various stages of a menstrual or oestrous cycle, where it plays an important role in embryo implantation and placentation. However, the expression and function of MUC1 in uterine tissues during early pregnancy in sheep after artificially-induced oestrous is not known. Therefore, we investigated the expression and function of MUC1 in the early pregnant and non-pregnant uterine tissues of sheep with natural oestrous or artificially-induced oestrous on days 10, 12, 14, 16 and 18 of the cycle by in situ hybridization, quantitative real-time polymerase chain reaction, immunohistochemical staining and western blotting methods...
December 20, 2017: Theriogenology
Nariaki Saito, Takefumi Iio, Yasunaga Yoshikawa, Hiromichi Ohtsuka, Koichi Orino
Lactoferrin (Lf) and transferrin (Tf) are iron-binding proteins that can bind various metal ions. This study demonstrates the heme-binding activity of bovine Lf and Tf using biotinylated hemin. When both proteins were coated on separate plate wells, each directly bound biotinylated hemin. On the other hand, when biotinylated hemin was immobilized on an avidin-coated plate, soluble native Lf bound to the immobilized biotinylated hemin whereas native Tf did not, suggesting that a conformational change triggered by coating on the plate allows the binding of denatured Tf with hemin...
December 28, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Ting Zhang, Lu Wang, Congwei Gao, Chaoyue Zhao, Yang Wang, Jianmin Wang
Hemin immobilized into copper-based metal-organic framework were successfully prepared and used as new electrode materials for sensitive electrochemical biosensing. X-ray diffraction patterns, Fourier transform infrared spectra, scanning electron microscope, UV-vis absorption spectroscopy, and cyclic voltammetry were used to characterize the resultant composites. Due to the interaction between the copper atom groups and hemin, the constrained environment in Cu-MOF-74 acts as a matrix to avoid the dimerization of enzyme molecule and retain its biological activity...
December 18, 2017: Nanotechnology
Alice Harding, Ulrike Gonder, Sarita J Robinson, StJohn Crean, Sim K Singhrao
Longitudinal monitoring of patients suggests a causal link between chronic periodontitis and the development of Alzheimer's disease (AD). However, the explanation of how periodontitis can lead to dementia remains unclear. A working hypothesis links extrinsic inflammation as a secondary cause of AD. This hypothesis suggests a compromised oral hygiene leads to a dysbiotic oral microbiome whereby Porphyromonas gingivalis, a keystone periodontal pathogen, with its companion species, orchestrates immune subversion in the host...
2017: Frontiers in Aging Neuroscience
Magdalena Makarska-Bialokoz
The binding interactions between hemin (Hmi) and bovine serum albumin (BSA) or human hemoglobin (HHb), respectively, have been examined in aqueous solution at pH=7.4, applying UV-vis absorption, as well as steady-state, synchronous and three-dimensional fluorescence spectra techniques. Representative results received for both BSA and HHb intrinsic fluorescence proceeding from the interactions with hemin suggest the formation of stacking non-covalent and non-fluorescent complexes in both the Hmi-BSA and Hmi-HHb systems, with highly possible concurrent formation of a coordinate bond between a group on the protein surface and the metal in Hmi molecule...
December 1, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Tatsuya Tada, Kohei Uechi, Isamu Nakasone, Zenji Miyazato, Takashi Shinzato, Kayo Shimada, Mitsuhiro Tsuchiya, Teruo Kirikae, Jiro Fujita
Small-colony variants (SCVs) were obtained from an Enterobacter cloacae clinical isolate in Okinawa, Japan. One variant showed auxotrophy for hemin with a deletion of 20 365 nucleotides, dosC-ydiK-mmuP-mmuM-tauA-tauB-tauC-tauD-hemB-yaiT-yaiV-ampH-yddQ-sbmA-yaiW-yaiY-yaiZ, including hemB, and was more resistant to aminoglycosides and carbapenems, but more susceptible to aztreonam, than the parent strain.
December 5, 2017: Journal of Medical Microbiology
Hui-Min Li, Ying-Lu Shi, Di Wen, Huan-Min Luo, Xi Lin, Fei Xiao
There is no effective drug for the therapy of acute carbon monoxide (CO) poisoning. The purpose of the present study was to investigate the potential preventive and therapeutic effects of hemin on an animal model of acute CO poisoning and to provide a potential therapeutic candidate drug. A total of 80 Kunming mice were randomly divided into four groups, namely the air control, acute CO poisoning, hemin-treatment + CO and hemin-pretreatment + CO groups (n=20 each). Furthermore, the mortality rate of mice, blood carboxyhaemoglobin (HbCO) concentration and serum malondialdehyde (MDA) concentration were measured, and pathological changes of the hippocampal area were determined using histochemical staining...
November 2017: Experimental and Therapeutic Medicine
Wen-Lan Wang, Yuan Min, Sheng-Song Yu, Wei Chen, Jie-Jie Chen, Xiao-Yang Liu, Han-Qing Yu
Extracellular electron transfer (EET) occurs from outer-membrane proteins to electron acceptors. Heme(ii) is the active center of outer-membrane proteins and delivers electrons to acceptors or mediators such as riboflavin, a redox active chromophore present in organisms. However, the EET mechanism via mediators, especially the electron transfer process from outer-membrane proteins to mediators, has not been well documented yet. In this work, the mechanism behind the electron transfer from heme(ii) to riboflavin is investigated by using in situ ultraviolet visible and fluorescence spectroelectrochemical analysis, which provides the information regarding the structural change and electrochemical characteristics of species in the electron transfer process...
November 30, 2017: Physical Chemistry Chemical Physics: PCCP
Chun-Hu Wu, Song-Kun Shyue, Tai-Ho Hung, Shin Wen, Chao-Chang Lin, Che-Feng Chang, Szu-Fu Chen
BACKGROUND: Inflammatory responses significantly contribute to neuronal damage and poor functional outcomes following intracerebral hemorrhage (ICH). Soluble epoxide hydrolase (sEH) is known to induce neuroinflammatory responses via degradation of anti-inflammatory epoxyeicosatrienoic acids (EET), and sEH is upregulated in response to brain injury. The present study investigated the involvement of sEH in ICH-induced neuroinflammation, brain damage, and functional deficits using a mouse ICH model and microglial cultures...
November 25, 2017: Journal of Neuroinflammation
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