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https://www.readbyqxmd.com/read/28107612/hemin-binding-by-porphyromonas-gingivalis-strains-is-dependent-on-the-presence-of-a-lps
#1
Minnie Rangarajan, Joseph Aduse-Opoku, Nikolay A Paramonov, Ahmed Hashim, Michael A Curtis
Porphyromonas gingivalis is a Gram-negative black pigmenting anaerobe unable to synthesise haem (Fe (II)-protoporphyrin IX) or hemin (Fe(III)-protoporphyrin IX-Cl) which are important growth/virulence-factors, and must therefore derive them from the host P. gingivalis expresses several proteinaceous hemin -binding-sites which are important in the binding/ transport of haem/hemin from the host. P. gingivalis also synthesises several virulence factors, namely cysteine-proteases Arg- and Lys-gingipains and two lipopolysaccharides (LPS), O-LPS and A-LPS...
January 20, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28105837/hrp-mimicking-dnazyme-catalyzed-in-situ-generation-of-polyaniline-to-assist-signal-amplification-for-ultrasensitive-surface-plasmon-resonance-biosensing
#2
Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. Meanwhile, polyaniline exhibits extraordinary electrical, electrochemical, and redox properties, as well as excellent SPR signal-enhancing ability. Herein, we report a novel ultrasensitive surface plasmon resonance (SPR) biosensor based on HRP-mimicking DNAzyme-catalyzed in situ formation of polyaniline for signal amplification, using bleomycin (BLM) as the proof-of-concept analyte...
January 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28102061/clinical-diversity-of-atopic-dermatitis-a-review-of-5-000-patients-at-a-single-institute
#3
Howard Chu, Jung U Shin, Chang Ook Park, Hemin Lee, Jungsoo Lee, Kwang Hoon Lee
PURPOSE: Atopic dermatitis (AD) is a chronic eczematous dermatitis that has a high prevalence and diverse clinical features. Although several hypotheses about its multifactorial pathogenesis have been suggested, the cause is not yet fully understood. A better understanding of the clinical features may helpful inelucidating the pathogenesis of AD. METHODS: This retrospective study analyzed the questionnaires, medical charts, and laboratory examination results of 5,000 patients diagnosed with AD at a single tertiary hospital in Korea...
March 2017: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/28078884/construction-of-three-dimensional-hemin-functionalized-graphene-hydrogel-with-high-mechanical-stability-and-adsorption-capacity-for-enhancing-photodegradation-of-methylene-blue
#4
Yuewu Zhao, Yuanjian Zhang, Anran Liu, Zhenzhen Wei, Songqin Liu
A three-dimensional hemin-functionalized graphene hydrogel (Hem/GH) was prepared by a facile self-assembly approach. The as-prepared Hem/GH showed good mechanical strength with a storage modulus of 609-642 kPa and a high adsorption capacity to organic dye contaminants (341 mg g-1 for rhodamine B). Moreover, Hem/GH could be used as photosensitizer for photocatalytic degradation of organic dyes, and showed superior photodegradation activity of methylene blue (MB) with respect to the counterparts such as graphene hydrogel (GH) and commercial catalyst P25...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28072547/mild-photochemical-biofunctionalization-of-glass-microchannels
#5
Rui Rijo Carvalho, Sidharam P Pujari, Digvijay Gahtory, Elwin X Vrouwe, Bauke Albada, Han Zuilhof
The ability to locally modify the inside of microfluidic channels with bioactive molecules is of ever-rising relevance. In this article, we show the direct photochemical coupling of a N-hydroxysuccinimide-terminated ω-alkene onto hydrogen-terminated silicon oxide, and its subsequent functionalization with a catalytically active DNAzyme. To achieve this local attachment of a DNAzyme, we prepared hydrogen-phenyl-terminated glass (H-Φ-glass) by the reaction of glass with H-SiPhCl2. The presence of a radical-stabilizing substituent on the Si atom (i...
January 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28071761/plasma-heme-induced-renal-toxicity-is-related-to-a-capillary-rarefaction
#6
Nahid Tabibzadeh, Céline Estournet, Sandrine Placier, Joëlle Perez, Héloïse Bilbault, Alexis Girshovich, Sophie Vandermeersch, Chantal Jouanneau, Emmanuel Letavernier, Nadjib Hammoudi, François Lionnet, Jean-Philippe Haymann
Severe hypertension can lead to malignant hypertension (MH) with renal thrombotic microangiopathy and hemolysis. The role of plasma heme release in this setting is unknown. We aimed at evaluating the effect of a mild plasma heme increase by hemin administration in angiotensin II (AngII)-mediated hypertensive rats. Prevalence of MH and blood pressure values were similar in AngII and AngII + hemin groups. MH rats displayed a decreased renal blood flow (RBF), increased renal vascular resistances (RVR), and increased aorta and interlobar arteries remodeling with a severe renal microcirculation assessed by peritubular capillaries (PTC) rarefaction...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28068626/effects-of-the-activation-of-heme-oxygenase-1-on-hormonal-and-metabolic-changes-in-rats-fed-a-high-fat-diet
#7
Tomasz Szkudelski, Kamila Dłużewicz, Jan Sadoch, Katarzyna Szkudelska
Heme oxygenase-1 (HO-1) is the inducible isoform of the heme oxygenase system, which catalyzes heme degradation. Up-regulation of this enzyme under pathological conditions is associated with beneficial effects in the whole organism. However, the potential of HO-1 in the alleviation of disturbances induced by a high-fat diet (HFD) is poorly elucidated. The present study was undertaken to determine the effects of pharmacological activation of HO-1 by hemin on some hormones and metabolic parameters in rats fed an HFD for 8 weeks...
January 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28068513/nrf2-dependent-induction-of-innate-host-defense-via-heme-oxygenase-1-inhibits-zika-virus-replication
#8
Hanxia Huang, Barry Falgout, Kazuyo Takeda, Kenneth M Yamada, Subhash Dhawan
We identified primary human monocyte-derived macrophages (MDM) as vulnerable target cells for Zika virus (ZIKV) infection. We demonstrate dramatic effects of hemin, the natural inducer of the heme catabolic enzyme heme oxygenase-1 (HO-1), in the reduction of ZIKV replication in vitro. Both LLC-MK2 monkey kidney cells and primary MDM exhibited hemin-induced HO-1 expression with major reductions of >90% in ZIKV replication, with little toxicity to infected cells. Silencing expression of HO-1 or its upstream regulatory gene, nuclear factor erythroid-related factor 2 (Nrf2), attenuated hemin-induced suppression of ZIKV infection, suggesting an important role for induction of these intracellular mediators in retarding ZIKV replication...
January 6, 2017: Virology
https://www.readbyqxmd.com/read/28065861/targeting-and-modulating-infarct-macrophages-with-hemin-formulated-in-designed-lipid-based-particles-improves-cardiac-remodeling-and-function
#9
Tamar Ben-Mordechai, David Kain, Radka Holbova, Natalie Landa, La-Paz Levin, Inbar Elron-Gross, Yifat Glucksam-Galnoy, Micha S Feinberg, Rimona Margalit, Jonathan Leor
Uncontrolled activation of pro-inflammatory macrophages after myocardial infarction (MI) accelerates adverse left ventricular (LV) remodeling and dysfunction. Hemin, an iron-containing porphyrin, activates heme oxygenase-1 (HO-1), an enzyme with anti-inflammatory and cytoprotective properties. We sought to determine the effects of hemin formulated in a macrophage-targeted lipid-based carrier (denoted HA-LP) on LV remodeling and function after MI. Hemin encapsulation efficiency was ~100% at therapeutic dose levels...
January 5, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28044053/plasma-levels-of-cyclooxygenase-2-cox-2-and-visfatin-during-different-stages-and-different-subtypes-of-migraine-headaches
#10
Chao Li, Qiushi Zhu, Qiu He, Junwei Wang, Fengzhi Wang, Hemin Zhang
BACKGROUND The aim of this study was to determine the plasma levels of cyclooxygenase-2 (COX-2) and visfatin in different stages and different subtypes of migraine headaches compared to a control group to elucidate the pathological mechanisms involved. MATERIAL AND METHODS We recruited a case-control cohort of 182 adult migraine patients and 80 age-matched and gender-matched healthy controls. The migraine patients were divided into two groups: the headache-attack-period group (Group A, n=77) and the headache-free-period group (Group B, n=105)...
January 3, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28031282/tonb-dependent-heme-hemoglobin-utilization-by-caulobacter-crescentus-huta
#11
Heloise Balhesteros, Yan Shipelskiy, Noah Long, Aritri Majumdar, Benjamin B Katz, Naara M Santos, Laura Leaden, Salete M Newton, Marilis V Marques, Phillip E Klebba
: Siderophore nutrition tests with Caulobacter crescentus strain NA1000 revealed that it utilized a variety of ferric hydroxamate siderophores, including asperchromes, ferrichromes, ferrichrome A, malonichrome and ferric aerobactin, as well as hemin and hemoglobin. C. crescentus did not transport ferrioxamine B or ferric catecholates. Because it did not use ferric enterobactin, the catecholate aposiderophore was an effective agent for iron deprivation. We determined the kinetics and thermodynamics of [(59)Fe]-apoferrichrome and [(59)Fe]-citrate binding and transport by NA1000...
December 28, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/28013165/in-vitro-cytotoxicity-evaluation-of-graphene-oxide-from-the-peroxidase-like-activity-perspective
#12
Wei Zhang, Ying Sun, Zhichao Lou, Lina Song, Yang Wu, Ning Gu, Yu Zhang
In this study, PEGylated graphene oxide (PEG-GO)-hemin composite structure was constructed. Hemin in the form of nanoscaled aggregates were immobilized on PEG-GO sheets by the π-π stacking super-molecular interaction. Via catalyzing the oxidation of chromogenic substrates, we elicited the obtained PEG-GO-Hemin composite sheets have much higher peroxidase-like activity compared to hemin or PEG-GO alone, which is due to the introduction of enzyme active center of hemin with high dispersity, the excellent affinity to organic substrate through π-π stacking and/or electrostatic adsorption and the rapid electron transfer capability of PEG-GO...
December 19, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28011635/hemoglobin-kirklareli-%C3%AE-h58l-a-new-variant-associated-with-fe-deficiency-and-increased-co-binding
#13
Emmanuel Bissé, Christine Schaeffer-Reiss, Alain Van Dorsselaer, Tchilabalo Dilezitoko Alayi, Thomas Epting, Karl Winkler, Andres S Benitez Cardenas, Jayashree Soman, Ivan Birukou, Premila P Samuel, John S Olson
Mutations in hemoglobin can cause a wide range of phenotypic outcomes, including anemia due to protein instability and red cell lysis. Uncovering the biochemical basis for these phenotypes can provide new insights into hemoglobin structure and function as well as identify new therapeutic opportunities. We report here a new hemoglobin α chain variant in a female patient with mild anemia, whose father also carries the trait and is from the Turkish city of Kirklareli. Both the patient and her father had a His58(E7)→Leu mutation in α1...
December 23, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28009088/horseradish-peroxidase-catalyzed-tyrosine-click-reaction
#14
Shinichi Sato, Kosuke Nakamura, Hiroyuki Nakamura
Efficiency of protein chemical modification on tyrosine residue with N-methyl luminol derivative was drastically improved using Horseradish peroxidase (HRP). In the previous method using hemin and H2O2, oxidative side reaction such as cysteine oxidation was problematic for functionalization of proteins on tyrosine selectively. Oxidative activation of N-methyl luminol derivatives with a minimum amount of H2O2 prevented the occurrence of oxidative side reaction under HRP-catalyzed conditions. As the probe for HRP-catalyzed protein modification, N-methyl luminol derivatives showed much higher efficiency than tyramide without inducing oligomerization of probe molecules...
December 23, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27997107/protein-directed-synthesis-of-bifunctional-adsorbent-catalytic-hemin-graphene-nanosheets-for-highly-efficient-removal-of-dye-pollutants-via-synergistic-adsorption-and-degradation
#15
Xiaopei Wang, Chen Hou, Wu Qiu, Yuepeng Ke, Qingchi Xu, Xiang Yang Liu, Youhui Lin
Herein, for the first time, we report a "green", one-pot reduction/decoration method for the synthesis of bifunctional adsorbent-catalytic hemin-graphene nanosheets by using a common available protein (bovine serum albumin, BSA) as both a reductant and a stabilizer. Our prepared nanosheets are highly stable and possess intrinsic peroxidase-like catalytic activity due to the decoration of BSA and hemin. Furthermore, benefiting from the combined advantages of graphene and BSA, these nanosheets are able to efficiently adsorb dye pollutants from aqueous solution...
December 27, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27995330/piceatannol-attenuates-homocysteine-induced-endoplasmic-reticulum-stress-and-endothelial-cell-damage-via-heme-oxygenase-1-expression
#16
Jin-Sang Kil, Sun-Oh Jeong, Hun-Taeg Chung, Hyun-Ock Pae
A growing body of evidence implicates endoplasmic reticulum (ER)-induced cellular dysfunction and apoptosis as important factors to a variety of diseases. In endothelial cells (ECs), the sulfur-containing amino acid homocysteine (Hcy) causes EC apoptosis and reactive oxygen species (ROS) generation through induction of ER stress. Here, we have investigated whether piceatannol (Pic), a resveratrol analog, could protect ECs against Hcy-induced apoptosis, oxidative stress and ER stress, with specific emphasis on heme oxygenase-1 (HO-1)...
December 20, 2016: Amino Acids
https://www.readbyqxmd.com/read/27984013/label-free-colorimetric-assay-for-t4-polynucleotide-kinase-phosphatase-activity-and-its-inhibitors-based-on-g-quadruplex-hemin-dnazyme
#17
Haisheng Liu, Changbei Ma, Jun Wang, Hanchun Chen, Kemin Wang
Here we report a new approach for label-free colorimetric assay of T4 polynucleotide kinase/phosphatase (PNKP) activity based on G-quadruplex/hemin DNAzyme. In the presence of T4 PNKP, the DNA primer with a 3'-phosphate can be dephosphorylated into a 3'-hydroxyl and initiate a primer elongation reaction to open the hairpin probe, and leading to releasing the G-quartets. Under optimal conditions, the proposed method exhibited a considerable performance with a detection limit of 0.01 U/mL. Furthermore, the present assay can be used to study the potential T4 PNKP inhibitor screening, making it promise to be applied in the fields of drug discovery...
October 27, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27975165/screening-somatic-cell-nuclear-transfer-parameters-for-generation-of-transgenic-cloned-cattle-with-intragenomic-integration-of-additional-gene-copies-that-encode-bovine-adipocyte-type-fatty-acid-binding-protein-a-fabp
#18
Yong Guo, Hejuan Li, Ying Wang, Xingrong Yan, Xihui Sheng, Di Chang, Xiaolong Qi, Xiangguo Wang, Yunhai Liu, Junya Li, Hemin Ni
Somatic cell nuclear transfer (SCNT) is frequently used to produce transgenic cloned livestock, but it is still associated with low success rates. To our knowledge, we are the first to report successful production of transgenic cattle that overexpress bovine adipocyte-type fatty acid binding proteins (A-FABPs) with the aid of SCNT. Intragenomic integration of additional A-FABP gene copies has been found to be positively correlated with the intramuscular fat content in different farm livestock species. First, we optimized the cloning parameters to produce bovine embryos integrated with A-FABP by SCNT, such as applied voltage field strength and pulse duration for electrofusion, morphology and size of donor cells, and number of donor cells passages...
December 14, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27974865/association-of-heme-oxygenase-1-with-lung-protection-in-malaria-associated-ali-ards
#19
Marcelo L M Pereira, Luana S Ortolan, Michelle K Sercundes, Daniela Debone, Oscar Murillo, Flávia A Lima, Claudio R F Marinho, Sabrina Epiphanio
Malaria is a serious disease, caused by the parasite of the genus Plasmodium, which was responsible for 440,000 deaths in 2015. Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is one of the main clinical complications in severe malaria. The murine model DBA/2 reproduces the clinical signs of ALI/ARDS in humans, when infected with Plasmodium berghei ANKA. High levels of HO-1 were reported in cases of severe malaria. Our data indicated that the HO-1 mRNA and protein expression are increased in mice that develop malaria-associated ALI/ARDS (MA-ALI/ARDS)...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27940243/sigch-an-extracytoplasmic-function-sigma-factor-of-porphyromonas-gingivalis-regulates-the-expression-of-cdhr-and-hmuyr
#20
Koki Ota, Yuichiro Kikuchi, Kentaro Imamura, Daichi Kita, Kouki Yoshikawa, Atsushi Saito, Kazuyuki Ishihara
Extracytoplasmic function (ECF) sigma factors play an important role in the bacterial response to various environmental stresses. Porphyromonas gingivalis, a prominent etiological agent in human periodontitis, possesses six putative ECF sigma factors. So far, information is limited on the ECF sigma factor, PGN_0319. The aim of this study was to investigate the role of PGN_0319 (SigCH) of P. gingivalis, focusing on the regulation of hmuY and hmuR, which encode outer-membrane proteins involved in hemin utilization, and cdhR, a transcriptional regulator of hmuYR...
December 8, 2016: Anaerobe
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