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https://www.readbyqxmd.com/read/28627599/hmox-1-inhibits-tgf-%C3%AE-induced-epithelial-mesenchymal-transition-in-the-mcf-7-breast-cancer-cell-line
#1
Xiaofeng Zhu, Shuichuan Huang, Lili Zeng, Jieyi Ma, Suhong Sun, Feng Zeng, Fanli Kong, Xiaoming Cheng
Epithelial‑mesenchymal transition (EMT) is a key mechanism underlying metastatic breast cancer. Reactive oxygen species (ROS) play an important role in EMT. Heme oxygenase‑1 (HMOX‑1) can reduce oxidative stress. However, the effect of HMOX‑1 on the EMT process in breast cancer cells is unknown. We treated the MCF‑7 breast cancer cell line with the HMOX‑1 inducer hemin and observed that hemin induced HMOX‑1 expression and inhibited migration, invasion and ROS generation in transforming growth factor‑β (TGF‑β)‑treated MCF‑7 cells using quantitative RT‑qPCR, western blotting, wound‑healing and cell invasion assays as well as fluorescent probe DCFDA...
June 14, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28625994/a-small-protein-hemp-is-a-transcriptional-activator-for-hemin-uptake-operon-in-burkholderia-multivorans-atcc-17616
#2
Takuya Sato, Shouta Nonoyama, Akane Kimura, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda
Iron and heme play very important roles in various metabolic functions in bacteria, and their intracellular homeostasis is maintained because high concentrations of free forms of these molecules greatly facilitate the Fenton reaction-mediated production of large amounts of reactive oxygen species that severely damage various biomolecules. Ferric uptake regulator (Fur) from Burkholderiamultivorans ATCC 17616 is an iron-responsive global transcriptional regulator, and its fur deletant exhibits pleiotropic phenotypes...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28622642/highly-sensitive-electrochemical-thrombin-aptasensor-based-on-peptide-enhanced-electrocatalysis-of-hemin-g-quadruplex-and-nanocomposite-as-nanocarrier
#3
Yongmei Wu, Lina Zou, Sheng Lei, Qian Yu, Baoxian Ye
In this work, we first conjugated a short peptide to thrombin binding aptamer (TBA) and bond hemin to the hybrid, effectively rendering hemin/G4-peptide more active over the original hemin/G4, so that a highly sensitive electrochemical thrombin (TB) aptasensor was developed based on it and PtNTs@rGO nanocomposite. It was the first report on the application of hemin/G4-peptide in electrochemical aptasensor. PtNTs@rGO with large surface area served as excellent nanocarrier for high loading of hemin/G4-peptide hybrids, resulting in the formation of hemin/G4-peptide-PtNTs@rGO bioconjugate as the secondary aptamer and further signal enhancement...
June 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28621518/a-photoelectrochemical-bioanalysis-platform-for-cells-monitoring-based-on-dual-signal-amplification-using-in-situ-generation-of-electron-acceptor-coupled-with-heterojunction
#4
Ruyan Li, Yue Zhang, Wenwen Tu, Zhihui Dai
Using in suit generation of electron acceptor coupled with heterojunction as dual signal amplification, a simple photoelectrochemical (PEC) bioanalysis platform was designed. The synergic effect between the PEC activities of carbon nitride (C3N4) nanosheets and PbS quantum dots (QDs) achieved almost 9 fold photocurrent intensity increment, compared with the C3N4 alone. After the G-quadruplex/hemin/Pt NPs with catalase-like activity towards H2O2 was introduced, oxygen was in situ generated and acted as electron donor, improving charge separation efficiency and further enhancing photocurrent response...
June 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28615713/exosomal-micrornas-in-giant-panda-ailuropoda-melanoleuca-breast-milk-potential-maternal-regulators-for-the-development-of-newborn-cubs
#5
Jideng Ma, Chengdong Wang, Keren Long, Hemin Zhang, Jinwei Zhang, Long Jin, Qianzi Tang, Anan Jiang, Xun Wang, Shilin Tian, Li Chen, Dafang He, Desheng Li, Shan Huang, Zhi Jiang, Mingzhou Li
The physiological role of miRNAs is widely understood to include fine-tuning the post-transcriptional regulation of a wide array of biological processes. Extensive studies have indicated that exosomal miRNAs in the bodily fluids of various organisms can be transferred between living cells for the delivery of gene silencing signals. Here, we illustrated the expression characteristics of exosomal miRNAs in giant panda breast milk during distinct lactation periods and highlighted the enrichment of immune- and development-related endogenous miRNAs in colostral and mature giant panda milk...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28588503/hepcidin-protects-neuron-from-hemin-mediated-injury-by-reducing-iron
#6
Yu-Fu Zhou, Chao Zhang, Guang Yang, Zhong-Ming Qian, Meng-Wan Zhang, Juan Ma, Fa-Li Zhang, Ya Ke
Hemin plays a key role in mediating secondary neuronal injury after intracerebral hemorrhage (ICH) and the cell toxicity of hemin is thought to be due to iron that is liberated when hemin is degraded. In a recent study, we demonstrated the iron regulatory hormone hepcidin reduces brain iron in iron-overloaded rats. Therefore, we hypothesized that hepcidin might be able to reduce iron and then protect neurons from hemin or iron-mediated neurotoxicity in hemin-treated neuronal cells. Here, we tested the hypothesis and demonstrated that ad-hepcidin and hepcidin peptide both have the ability to suppress the hemin-induced increase in LDH release and apoptotic cell numbers, to reduce cell iron and ferritin contents, and to inhibit expression of transferrin receptor 1, divalent metal transporter 1, and ferroportin 1 in hemin-treated neurons...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28583303/keap1-knockdown-in-melanocytes-induces-cell-proliferation-and-survival-via-ho-1-associated-%C3%AE-catenin-signaling
#7
Ji Young Kim, Hemin Lee, Eun Jung Lee, Mikyoung Kim, Tae-Gyun Kim, Hyoung-Pyo Kim, Sang Ho Oh
BACKGROUND: Nrf2-Keap1 signaling pathway protects cells against photo-oxidative stress. Yet in recent works, its role in melanogenesis together with cell protection functions against oxidative stress has been gaining interest. However, its effect on melanogenesis still has contradictory results from different studies. OBJECTIVE: The aims of our study were to investigate the effect of Keap1 silencing in melanocyte on melanogenesis and its associated mechanism. METHODS: Primary human epidermal melanocytes and melan-a cell line were used for this experiment...
May 18, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28580587/heme-oxygenase-1-directly-binds-stat3-to-control-the-generation-of-pathogenic-th17-cells-during-neutrophilic-airway-inflammation
#8
Xiaoliang Lin, Jiajia Lv, Jie Lv, Caixia Di, Yanjie Zhang, Tong Zhou, Junling Liu, Zhenwei Xia
BACKGROUND: Specific JAK/STAT pathways play a critical role in the functional differentiation of distinct Th subsets. Previously, we showed that HO-1, a stress-inducible protein, inhibits Th17 cell differentiation and alleviates neutrophilic airway inflammation, but the responsible molecular basis remains unclear. METHODS: We employed Th17-skewing differentiation and NEA mouse models to study the role of HO-1 regulating IL-6-STAT3-RORγt/SOCS3 signaling pathway to control Th17 cell-mediated neutrophilic airway inflammation...
June 5, 2017: Allergy
https://www.readbyqxmd.com/read/28575989/highly-water-soluble-ph-sensitive-and-biocompatible-pamam-dendrizyme-to-maintain-catalytic-activity-in-complex-medium
#9
Longgang Wang, Yanshuai Cui, Shengfu Chen, Guangzhi Wang, Dawei Gao, Yanping Liu, Qiaoyue Luo, Zhiwei Liu, Xuwu Zhang
The dendrimer based synthetic mimetic enzyme has been drawing great attention. However, this mimetic enzyme is different from the natural enzymes, which are pH sensitive, biocompatible and keep their catalytic activity in biological complex medium. A single zwitterionic layer composed by primary amine and carboxyl groups may be a useful method to obtain these properties. Herein, we report a novel facile method to prepare a mimetic enzyme. The complexes of generation 5 poly(amido amine) dendrimers (G5 PAMAM) with free hemin (G5Hs) were modified by the maleic anhydride and cysteamine...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28574021/isolation-of-bacteria-from-diabetic-foot-ulcers-with-special-reference-to-anaerobe-isolation-by-simple-two-step-combustion-technique-in-candle-jar
#10
Jayeeta Haldar, Poulami Mukherjee, Satinath Mukhopadhyay, Prasanta Kumar Maiti
BACKGROUND & OBJECTIVES: Although polymicrobial infections involving both aerobic and anaerobic bacteria are very common in diabetic foot ulcers, in many centres of developing countries, anaerobes are rarely isolated due to technical difficulties. This can be overcome by using a new simple, innovative technique of a combination of candle combustion and use of acidified copper-coated steel wool, as reported here. METHODS: In-house developed method was used in a prospective clinico-microbiological study for anaerobes from randomly selected 43 patients with diabetic foot ulcers along with conventional method of anaerobic culture in GasPak system and aerobic culture by standard laboratory procedures...
January 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28561988/electron-hopping-across-hemin-doped-serum-albumin-mats-on-centimeter-length-scales
#11
Nadav Amdursky, Xuhua Wang, Paul Meredith, D Jason Riley, David J Payne, Donal D C Bradley, Molly M Stevens
Exploring long-range electron transport across protein assemblies is a central interest in both the fundamental research of biological processes and the emerging field of bioelectronics. This work examines the use of serum-albumin-based freestanding mats as macroscopic electron mediators in bioelectronic devices. In particular, this study focuses on how doping the protein mat with hemin improves charge-transport. It is demonstrated that doping can increase conductivity 40-fold via electron hopping between adjacent hemin molecules, resulting in the highest measured conductance for a protein-based material yet reported, and transport over centimeter length scales...
May 31, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28561084/a-label-free-colorimetric-biosensor-for-sensitive-detection-of-vascular-endothelial-growth-factor-165
#12
Huige Zhang, Liang Peng, Maoxing Li, Ji Ma, Shengda Qi, Hongli Chen, Lei Zhou, Xingguo Chen
Sensitive detection of a low abundant protein is essential for biomedical research and clinical diagnostics. Herein, we develop a label-free colorimetric biosensor for the sensitive detection of recombinant human vascular endothelial growth factor-165 (VEGF165). This biosensor consists of an aptamer-based hairpin probe, an assistant DNA-trigger duplex and a linear template. In the presence of VEGF165, the specific binding of VEGF165 with the aptamer-based hairpin probe results in the opening of a hairpin probe and the opened hairpin probe subsequently hybridizes with the single-stranded region of the assistant DNA-trigger duplex to initiate the strand displacement amplification (SDA) to yield abundant triggers...
May 31, 2017: Analyst
https://www.readbyqxmd.com/read/28560283/dataset-on-characterization-of-hemin-azide-derivative-and-dna-oligonucleotide-hemin-conjugate
#13
J Kosman, A Stanislawska, A Gluszynska, B Juskowiak
In this article newly synthesized azide derivative of hemin and DNA-hemin conjugate are characterized. Hemin-azide was purified using HPLC and characterized using elemental analysis, IR and NMR. The DNA-hemin conjugate was obtained via click chemistry [1] and click reaction was carried out using traditional Cu-catalyzed and Cu-free approaches. The final product was successfully obtained using Cu-free cycloaddition. The identity of product was confirmed using Maldi TOF spectrometry. Obtained hemin-DNA conjugate exhibited peroxidase-like activity...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28552822/changes-in-dna-methylation-of-erythroid-specific-genes-in-k562-cells-exposed-to-catechol-in-long-term
#14
Chun-Hong Yu, Ning-Xuan Cui, Yan Wang, Ying Wang, Wen-Juan Liu, Meng Gong, Xiao Zhao, Long Rong, Zong-Chun Yi
Catechol is one of phenolic metabolites of benzene that is a general occupational hazard and a ubiquitous environmental air pollutant. Catechol also occurs naturally in fruits, vegetables and cigarettes. Previous studies have revealed that 72h exposure to catechol improved hemin-induced erythroid differentiation of K562 cells accompanied with elevated methylation in erythroid specific genes. In present study, K562 cells were treated with 0, 10 or 20μM catechol for 1-4weeks, hemin-induced hemoglobin synthesis increased in a concentration- and time-dependent manner and the enhanced hemoglobin synthesis was relatively stable...
May 25, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28551230/attributes-of-lipid-oxidation-due-to-bovine-myoglobin-hemoglobin-and-hemolysate
#15
Jie Yin, Wenjing Zhang, Mark P Richards
Bovine hemolysate was purified by size exclusion chromatography, and one high molecular weight protein was detected relative to the hemoglobin (Hb) fraction. Purified Hb promoted lipid oxidation in washed muscle slightly but significantly better than hemolysate, which may have been due to the absence of catalase and peroxiredoxin in the purified Hb. Purified Hb auto-oxidized to metHb more rapidly than Hb in the hemolysate (P<0.05). OxyHb promoted lipid oxidation in washed muscle more effectively compared to oxyMb (P<0...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28543864/modulation-of-heme-oxygenase-1-expression-and-activity-affects-streptozotocin-induced-diabetic-nephropathy-in-rats
#16
Marwa A M Ali, Gehan H Heeba, Azza A K El-Sheikh
Heme oxygenase (HO)-1 has exhibited nephro-protective actions in different animal models; however, its full mechanistic potential in diabetic nephropathy (DN) has not yet been elucidated. Hence, the present study has been undertaken by inducing DN in rats using streptozotocin (50mg/kg i.p.), with or without either HO-1 inducer; hemin (HM; 40 μmol/kg, s.c.), or HO-1 blocker; zinc protoporphyrin-IX (ZnPP; 50 μmol/kg, i.p.), for one month. Compared to control, rats with DN suffered from hyperglycemia and hyperlipidemia, with signs of renal damage, as assessed by distortion in renal histopathological architecture and kidney function...
May 23, 2017: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/28542806/structural-and-functional-study-of-a-simple-rapid-and-label-free-dnazyme-based-dna-biosensor-for-optimization-activity
#17
Narges Shahbazi, Saman Hosseinkhani, Khosro Khajeh, Bijan Ranjbar
Peroxidase-mimicking DNAzyme has a potential to self-assemble into a G-quadruplex and shows peroxidase activity. In comparison to proteins, peroxidase-mimicking DNAzyme is less expensive and more stable. Herein, it is used in fabricating non-labeling biosensors. This paper investigates the structural and functional properties of a DNA biosensor based on split DNAzyme with a detection limit in nM range (9.48 nM). Two halves of DNAzyme were linked by a complementary sequence of DNA target. Hybridization of the DNA target pulled two DNAzyme halves apart and peroxidase activity decreased...
May 20, 2017: Biopolymers
https://www.readbyqxmd.com/read/28540303/identifying-the-genes-responsible-for-iron-limited-condition-in-riemerella-anatipestifer-ch-1-through-rna-seq-based-analysis
#18
MaFeng Liu, Mi Huang, DeKang Zhu, MingShu Wang, RenYong Jia, Shun Chen, KunFeng Sun, Qiao Yang, Ying Wu, Francis Biville, AnChun Cheng
One of the important elements for most bacterial growth is iron, the bioavailability of which is limited in hosts. Riemerella anatipestifer (R. anatipestifer, RA), an important duck pathogen, requires iron to live. However, the genes involved in iron metabolism and the mechanisms of iron transport are largely unknown. Here, we investigated the transcriptomic effects of iron limitation condition on R. anatipestifer CH-1 using the RNA-Seq and RNA-Seq-based analysis. Data analysis revealed genes encoding functions related to iron homeostasis, including a number of putative TonB-dependent receptor systems, a HmuY-like protein-dependent hemin (an iron-containing porphyrin) uptake system, a Feo system, a gene cluster related to starch utilization, and genes encoding hypothetical proteins that were significantly upregulated in response to iron limitation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28536715/hemin-catalyzed-sulfonium-ylide-formation-and-subsequently-reactant-controlled-chemoselective-rearrangements
#19
Xiaofei Xu, Chang Li, Mingteng Xiong, Zhihao Tao, Yuanjiang Pan
Hemin-catalyzed competing routes of [1,2]-Stevens and Sommelet-Hauser rearrangements of benzyl sulfonium ylides through an iron porphyrin carbenoid were established. These rearrangements can be controlled by the electronic property of the substituents on the benzyl group together with solvent effects. Electron-donating or weak electron-withdrawing groups lead to the [1,2]-Stevens rearrangement, whereas strong electron-withdrawing groups at the para-position favor the latter pathway under aqueous conditions.
June 6, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28534562/k-junction-structure-mediated-exponential-signal-amplification-strategy-for-microrna-detection-in-electrochemiluminescence-biosensor
#20
Xue Li, Anyi Chen, Yaqin Chai, Ruo Yuan
Based on the novel designed K-junction structure, an economic and efficient exponential signal amplification strategy with simple protocol combining hemin/G-quadruplex, a mimetic peroxidase, as a catalyzer was proposed and utilized in an electrochemiluminescence biosensor for sensitive microRNA detection. It was noteworthy that the K-junction structure was formed with guanine-rich reporter DNA and substrate DNA modified by phosphate at the 5'-terminus. Thus, the activity of the reporter DNA could be inhibited and the recognition domain for the target microRNA constructed through the K-junction structure formed...
May 23, 2017: Analyst
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