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https://www.readbyqxmd.com/read/28817219/lycium-barbarum-polysaccharide-attenuates-chemotherapy-induced-ovarian-injury-by-reducing-oxidative-stress
#1
Dong-Mei Yang, Jing-Qin Zhang, Yi-Fei Fei
AIM: We aimed to examine the effects of Lycium barbarum polysaccharide (LBP) on ovarian damage induced by cyclophosphamide (CTX) and to investigate the underlying mechanism. METHODS: A total of 240 female Sprague-Dawley rats were randomly divided into five groups: the control group, the CTX-induced ovarian injury (OI) group, and three LBP groups. Different concentrations of LBP solution were administered to the LBP groups by gastric infusion for 15 days, and the OI group and LBP groups were then subjected to CTX treatment for another 15 days...
August 17, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28816586/oxidative-stress-in-the-visceral-fat-is-elevated-in-postmenopausal-women-with-gynecologic-cancer
#2
Megumi Narumi, Kazuhiro Takahashi, Hizuru Yamatani, Manabu Seino, Keiko Yamanouchi, Tsuyoshi Ohta, Toshifumi Takahashi, Hirohisa Kurachi, Satoru Nagase
OBJECTIVES: This study aimed to evaluate differences in oxidative stress of visceral fat between premenopausal and postmenopausal women and clarify the antioxidant effect of estrogen on adipocytes. MATERIALS AND METHODS: Abdominal subcutaneous and omental visceral adipose tissues were obtained from 38 patients who underwent gynecological surgery. We measured the sizes of the adipocytes and evaluated the lipid peroxidation levels in the adipose tissues. We investigated whether estrogen inhibited the intracellular reactive oxygen species (ROS) production that was induced by hydrogen peroxide (H2O2) in 3T3-L1 adipocytes...
August 17, 2017: Journal of Women's Health
https://www.readbyqxmd.com/read/28815214/chlamydomonas-reinhardtii-lfo1-is-an-isdg-family-heme-oxygenase
#3
Lisa J Lojek, Allison J Farrand, Jennifer H Wisecaver, Crysten E Blaby-Haas, Brian W Michel, Sabeeha S Merchant, Antonis Rokas, Eric P Skaar
Heme is essential for respiration across all domains of life. However, heme accumulation can lead to toxicity if cells are unable to either degrade or export heme or its toxic by-products. Under aerobic conditions, heme degradation is performed by heme oxygenases, enzymes which utilize oxygen to cleave the tetrapyrrole ring of heme. The HO-1 family of heme oxygenases has been identified in both bacterial and eukaryotic cells, whereas the IsdG family has thus far been described only in bacteria. We identified a hypothetical protein in the eukaryotic green alga Chlamydomonas reinhardtii, which encodes a protein containing an antibiotic biosynthesis monooxygenase (ABM) domain consistent with those associated with IsdG family members...
July 2017: MSphere
https://www.readbyqxmd.com/read/28814160/elucidation-of-molecular-mechanism-of-stability-of-the-heme-regulated-eif2%C3%AE-kinase-upon-binding-of-its-ligand-hemin-in-its-catalytic-kinase-domain
#4
Varsha Bhavnani, Kaviraj Swarnendu, Priyabrata Panigrahi, C G Suresh, SuneelShekar Yapara, Jayanta Pal
The eIF2α kinase activity of the heme-regulated inhibitor (HRI) is regulated by heme which makes it a unique member of the family of eIF2α kinases. Since heme concentrations create an equilibrium for the kinase to be active/inactive, it becomes important to study the heme binding effects upon the kinase and understanding its mechanism of functionality. In the present study, we report the thermostability achieved by the catalytic kinase domain of HRI (HRI.CKD) upon ligand (heme) binding. Our CD data demonstrates that the HRI...
August 17, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28813685/purification-and-characterization-of-two-novel-peroxidases-from-the-dye-decolorizing-fungus-bjerkandera-adusta-strain-cx-9
#5
Khelifa Bouacem, Hatem Rekik, Nadia Zaraî Jaouadi, Bilal Zenati, Sidali Kourdali, Mohamed El Hattab, Abdelmalek Badis, Rachid Annane, Samir Bejar, Hocine Hacene, Amel Bouanane-Darenfed, Bassem Jaouadi
Two extracellular peroxidases from Bjerkandera adusta strain CX-9, namely a lignin peroxidase (called LiP BA45) and manganese peroxidase (called MnP BA30), were purified simultaneously by applying successively, ammonium sulfate precipitation-dialysis, Mono-S Sepharose anion-exchange and Sephacryl S-200 gel filtration and biochemically characterized. The sequence of their NH2-terminal amino acid residues showed high homology with those of fungi peroxidases. MALDI-TOF/MS analysis revealed that the purified enzymes MnP BA30 and LiP BA45 were a monomers with a molecular masses 30125...
August 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28813137/site-a-mediated-partial-unfolding-of-cytochrome-c-on-cardiolipin-vesicles-is-species-dependent-and-does-not-require-lys72
#6
Margaret M Elmer-Dixon, Bruce E Bowler
Measurements at pH 8 allow evaluation of binding of 100% cardiolipin vesicles to site A of cytochrome c without interference from other known binding sites. Site A encompasses Lys72, Lys73, Lys86 and Lys87, located in or adjacent to Ω-loop D (residues 70-85), which positions Met80 for binding to the heme. Binding of cytochrome c to cardiolipin disrupts Met80 heme binding, permitting peroxidase activity. Cardiolipin binding to yeast iso-1-cytochrome c versus human cytochrome c is compared to assess how cardiolipin binding to site A has evolved for cytochrome c from species that do not have a complete intrinsic apoptotic pathway to species that do...
August 16, 2017: Biochemistry
https://www.readbyqxmd.com/read/28811700/primary-myoglobinuria-differentiate-myoglobinuria-from-hemoglobinuria
#7
Dhiraj J Trivedi, Shrirang P Kulkarni, Rakesh Mudaraddi
Myoglobin is dark red colour heme containing protein, stored in muscle. Change in permeability of myolemma causes myoglobin leak in plasma, which is cleared by kidney swiftly. Differentiating myoglobinuria from hemoglobinuria is important. Clinicians concern over myoglobinuria is to protect the patient from acute renal disease. We present a case of primary myoglobinuria, its clinical symptoms, diagnosis and treatment.
July 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28811386/trimetazidine-protects-retinal-ganglion-cells-from-acute-glaucoma-via-the-nrf2-ho-1-pathway
#8
Peixing Wan, Wenru Su, Yingying Zhang, Zhidong Li, Caibin Deng, Yehong Zhuo
Acute glaucoma is one of the leading causes of irreversible vision impairment characterized by the rapid elevation of intraocular pressure and consequent retinal ganglion cell (RGC) death. Oxidative stress and neuro-inflammation have been considered critical for the pathogenesis of RGC death in acute glaucoma. Trimetazidine (TMZ), an anti-ischemic drug, possesses anti-oxidative and anti-inflammatory properties, contributing to its therapeutic potential in tissue damage. However, the role of TMZ in acute glaucoma and the underlying molecular mechanisms remain elusive...
August 15, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28811208/macromolecular-crowding-induces-molten-globule-state-in-the-native-myoglobin-at-physiological-ph
#9
Khalida Nasreen, Shahzaib Ahamad, Faizan Ahmad, Md Imtaiyaz Hassan, Asimul Islam
Here, we report the formation of molten globule state of the native myoglobin in crowded environment. We have used Soret absorption spectroscopy and far-UV circular dichroism to monitor changes in tertiary and secondary structures of myoglobin, respectively. Our results reveal that in the presence of ficoll 70, the secondary structure of myoglobin remains unchanged while tertiary structure is lost significantly. 1-anilinonaphthalene-8-sulfonate binding experiments showed that myoglobin in the presence of various concentrations of ficoll 70, has newly exposed hydrophobic surfaces...
August 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28810706/effects-of-trigonelline-inhibition-of-the-nrf2-transcription-factor-in-vitro-on-echinococcus-granulosus
#10
Wenjuan Qin, Dongfang Guan, Rongji Ma, Rentan Yang, Guoqiang Xing, Hongjuan Shi, Guangyao Tang, Jiajie Li, Hailong Lv, Yufeng Jiang
The aim of this study was to investigate the impact of trigonelline (TRG) on Echinococcus granulosus, and to explore the inhibition impact of nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway on E. granulosus protoscoleces. Echinococcus granulosus protoscoleces were incubated with various concentrations of TRG, and then Nrf2 protein expression and its localization in protoscoleces were detected by western blot analysis and immunofluorescence assay, respectively. Reactive oxygen species (ROS) level in protoscoleces was measured using ROS detection kit...
August 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28809552/characterization-of-2-oxindole-forming-heme-enzyme-mare-expanding-the-functional-diversity-of-the-tryptophan-dioxygenase-superfamily
#11
Yuyang Zhang, Yi Zou, Nelson L Brock, Tingting Huang, Yingxia Lan, Xiaozheng Wang, Zixin Deng, Yi Tang, Shuangjun Lin
3-Substituted 2-oxindoles are important structural motifs found in many biologically active natural products and pharmaceutical lead compounds. Here, we report an enzymatic formation of the 3-substituted 2-oxindoles catalyzed by MarE in the maremycin biosynthetic pathway in Streptomyces sp. B9173. MarE is a homologue of Fe(II)/heme-dependent tryptophan 2,3-dioxygenases (TDOs). Typical TDOs usually catalyze the insertion of two oxygen atoms from O2 into an indole ring to generate N-formylkynurenine (NFK)-like products...
August 15, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28808219/spindle-cell-carcinoma-of-the-mandible-a-case-report
#12
Long Xie, Heming Wu, Siyu Liu, Hongwei Li
Spindle cell carcinoma is a rare highly malignant squamous cell carcinoma. Here, we describe a case of a 74-year-old Chinese female who presented with a 2-week history of pain and swelling in the left retromolar region. Surgical resection and titanium plate prosthesis were performed and histological analysis revealed spindle squamous cell carcinoma.
January 19, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28808058/reductions-in-the-mitochondrial-abc-transporter-abcb10-affect-the-transcriptional-profile-of-heme-biosynthesis-genes
#13
Alexandra Seguin, Naoko Takahashi-Makise, Yvette Y Yien, Nicholas C Huston, Jared C Whitman, Gabriel Musso, Jared A Wallace, Thomas Bradley, Hector Bergonia, Martin D Kafina, Mitsuyo Matsumoto, Kazuhiko Igarashi, John D Phillips, Barry H Paw, Jerry Kaplan, Diane M Ward
ATP-binding cassette subfamily B member 10 (Abcb10) is a mitochondrial ABC transporter that complexes with mitoferrin1 and ferrochelatase to enhance heme biosynthesis in developing red blood cells. Reductions in Abcb10 levels have been shown to reduce mitoferrin1 protein levels and iron import into mitochondria resulting in reduced heme biosynthesis. As an ABC transporter, Abcb10 binds and hydrolyzes ATP, but its transported substrate is unknown. Here, we determined that decreases in Abcb10 did not result in protoporphyrin IX accumulation in morphant treated zebrafish embryos nor in differentiated Abcb10-specific shRNA murine Friend erythroleukemia (MEL) cells in which Abcb10 was specifically silenced with shRNA...
August 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28807985/inhaled-carbon-monoxide-from-toxin-to-therapy
#14
Dean R Hess
Carbon monoxide (CO) is usually recognized as a toxic gas that can be used to assess lung function in the pulmonary function laboratory. The toxicity of CO relates to its high affinity for hemoglobin and other heme molecules, producing carboxyhemoglobin (HbCO). Despite that blood HbCO levels are commonly measured in patients with CO poisoning, the clinical presentation often does not correlate with the HbCO level, and clinical improvement in the patient's condition does not correlate with HbCO clearance. In patients with CO poisoning, administration of 100% O2 is standard practice...
August 14, 2017: Respiratory Care
https://www.readbyqxmd.com/read/28807731/time-resolved-generation-of-membrane-potential-by-ba3-cytochrome-c-oxidase-from-thermus-thermophilus-coupled-to-single-electron-injection-into-the-o-and-oh-states
#15
Sergey A Siletsky, Ilya Belevich, Nikolay P Belevich, Tewfik Soulimane, Mårten Wikström
Two electrogenic phases with characteristic times of ~14μs and ~290μs are resolved in the kinetics of membrane potential generation coupled to single-electron reduction of the oxidized "relaxed" O state of ba3 oxidase from T. thermophilus (O→E transition). The rapid phase reflects electron redistribution between CuA and heme b. The slow phase includes electron redistribution from both CuA and heme b to heme a3, and electrogenic proton transfer coupled to reduction of heme a3. The distance of proton translocation corresponds to uptake of a proton from the inner water phase into the binuclear center where heme a3 is reduced, but there is no proton pumping and no reduction of CuB...
August 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28807201/overexpression-of-thioredoxin1-enhances-functional-recovery-in-a-mouse-model-of-hind-limb-ischemia
#16
Inam A Shaikh, Muhammad Tipu Rishi, Mark Youssef, Vaithinathan Selvaraju, Mahesh Thirunavukkarasu, Gopi Ukani, Rajesh Lakshmanan, J Alexander Palesty, Nilanjana Maulik
BACKGROUND: There is keen interest in finding nonsurgical treatments for peripheral vascular disease (PVD). Previously, we demonstrated that selective activation of Thioredoxin1 (Trx1), a 12-kDa cytosolic protein, initiates redox-dependent signaling and promotes neovascularization after ischemic heart disease. Therefore, Trx1 might possess immense potential to not only treat murine hind limb ischemia (HLI) through effective angiogenesis but also provide PVD patients with nonsurgical therapy to enhance neovascularization and improve blood perfusion...
August 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28806787/antagonism-of-proteasome-inhibitor-induced-heme-oxygenase-1-expression-by-pink1-mutation
#17
Xiang-Jun Sheng, Hunag-Ju Tu, Wei-Lin Chien, Kai-Hsiang Kang, Dai-Hua Lu, Horng-Huei Liou, Ming-Jen Lee, Wen-Mei Fu
PTEN-induced putative kinase 1 (PINK1) is an integral protein in the mitochondrial membrane and maintains mitochondrial fidelity. Pathogenic mutations in PINK1 have been identified as a cause of early-onset autosomal recessive familial Parkinson's disease (PD). The ubiquitin proteasome pathway is associated with neurodegenerative diseases. In this study, we investigated whether mutations of PINK1 affects the cellular stress response following proteasome inhibition. Administration of MG132, a peptide aldehyde proteasome inhibitor, significantly increased the expression of heme oxygenase-1 (HO-1) in rat dopaminergic neurons in the substantia nigra and in the SH-SY5Y neuronal cell line...
2017: PloS One
https://www.readbyqxmd.com/read/28805232/the-protective-effect-of-heme-oxygenase-1-against-intestinal-barrier-dysfunction-in-cholestatic-liver-injury-is-associated-with-nf-%C3%AE%C2%BAb-inhibition
#18
Lijing Zhang, Zhenling Zhang, Bojia Liu, Yanling Jin, Yan Tian, Yi Xin, Zhijun Duan
Heme oxygenase-1 (HO-1) is reported to protect against liver injury, but little is known about its effect on the intestinal barrier in cholestatic liver injury. In this study, we investigated the effects of HO-1 and its enzymatic by-product on intestinal barrier dysfunction in bile duct ligation (BDL) rats and explored the possible mechanism. The HO-1 inducer cobalt protoporphyrin (CoPP) and carbon monoxide-releasing molecule-2 (CORM-2) were used; the expression levels of tight junction (TJ) proteins, intestinal inflammation and NF-κB p65 were measured...
August 9, 2017: Molecular Medicine
https://www.readbyqxmd.com/read/28805222/heme-isomers-substantially-affect-heme-s-electronic-structure-and-function
#19
Kasper P Kepp
Inspection of heme protein structures in the protein data bank reveals four isomers of heme characterized by different relative orientations of the vinyl side chains; remarkably, all these have been reported in multiple protein structures. Density functional theory computations explain this as due to similar energy of the isomers but with a sizable (25 kJ mol(-1)) barrier to interconversion arising from restricted rotation around the conjugated bonds. The four isomers, EE, EZ, ZE, and ZZ, were then investigated as 4-coordinate hemes, as 5-coordinate deoxyhemes, in 6-coordinate O2-adducts of globins and as compound I intermediates typical of heme peroxidases...
August 14, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28803470/effect-of-dmso-on-protein-structure-and-interactions-assessed-by-collision-induced-dissociation-and-unfolding
#20
Daniel S-H Chan, Madeline E Eve Kavanagh, Kirsty J McLean, Andrew William Munro, Dijana Matak-Vinkovic, Anthony G Coyne, Chris Abell
Native mass spectrometry is an ideal technique to investigate the effects of DMSO on protein structure and interac-tions. Given the frequent use of DMSO in biochemical and biophysical assays, it is desirable to understand the influence of DMSO concentration on the dissociation or unfolding behavior of proteins. In this study, the effects of DMSO on the structure and interac-tions of avidin and Mycobacterium tuberculosis (Mtb) CYP142A1 were assessed through collision-induced dissociation (CID) and collision-induced unfolding (CIU) as monitored by nanoelectrospray ionization-ion mobility-mass spectrometry (nESI-IM-MS)...
August 13, 2017: Analytical Chemistry
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