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https://www.readbyqxmd.com/read/28342993/teratogenic-responses-of-zebrafish-embryos-to-decabromodiphenyl-ether-bde-209-in-the-presence-of-nano-sio2-particles
#1
Shu-Ju Chao, Chin Pao Huang, Pei-Chung Chen, Chihpin Huang
This study investigated the influence of nano-SiO2 particles (nSiO2) on the teratogenic responses of zebrafish embryos to decabromodiphenyl ether (BDE-209). Zebrafish embryos were exposed to BDE-209 in the absence and presence of nSiO2 for 96 h post fertilization (hpf). Results showed that formation of nSiO2-BDE-209 associates promoted both extracellular and intracellular uptake of BDE-209 by zebrafish embryos, thereby increasing the bioconcentration of BDE-209 on the chorion surface and the embryos. Results also showed embryos delay hatching temporarily when co-exposure to BDE-209 and nSiO2 at 60 hpf...
March 20, 2017: Chemosphere
https://www.readbyqxmd.com/read/28342100/erratum-to-yolk-sac-tumour-a-rare-cause-of-raised-serum-alpha-foetoprotein-in-a-young-child-with-a-large-liver-mass
#2
Annemieke S Littooij, Kieran McHugh, M Beth McCarville, Neil J Sebire, Armita Bahrami, Derek J Roebuck
No abstract text is available yet for this article.
March 24, 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/28340455/toxicological-and-behavioral-responses-as-a-tool-to-assess-the-effects-of-natural-and-synthetic-dyes-on-zebrafish-early-life
#3
Flavia R Abe, Jacqueline N Mendonça, Luiz A B Moraes, Gisele A R de Oliveira, Carlos Gravato, Amadeu M V M Soares, Danielle P de Oliveira
Organic dyes extracted from natural sources have been widely used to develop safety and eco-friendly dyes as an alternative to synthetic ones, since the latter are usually precursors of mutagenic compounds. Thereby, toxicity tests to non-target organisms are critical step to develop harmless dyes to environment and in this context, zebrafish early life stages are becoming an important alternative model. We aimed to assess the toxic effects of the synthetic dye Basic Red 51 (BR51, used in cosmetic industry), the natural dye erythrostominone (ERY, a potential commercial dye extracted from fungi) and its photodegradation product (DERY), using zebrafish early life assays...
March 9, 2017: Chemosphere
https://www.readbyqxmd.com/read/28339819/thermal-treatments-prior-to-and-during-the-beginning-of-incubation-affects-development-of-the-broiler-embryo-and-yolk-sac-membranes-and-live-performance-and-carcass-characteristics
#4
Y M Lin, S Druyan, S Yahav, J Brake
This study evaluated temperature during preincubation and embryonic day 0 (E0) E0 to E5 of incubation on broiler embryo development and subsequent live performance. Freshly laid eggs from a single 41-wk-old Ross 708 broiler breeder flock produced on a single day were weighed individually for weight matching purposes, stored overnight, and assigned to 4 treatment combinations of 2 preincubation temperatures (23.9 or 29.4°C) × 2 E0 to E5 temperatures (38.1 or 37.5°C). The 29.4°C preincubation temperature decreased (P ≤ 0...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28333368/morphological-specializations-of-the-yolk-sac-for-yolk-processing-in-embryonic-corn-snakes-pantherophis-guttatus-colubridae
#5
Kathryn G Powers, Daniel G Blackburn
Non-avian reptiles commonly are assumed to be like birds in their overall patterns of development. However, colubrid corn snakes (Pantherophis guttatus) have mechanisms of yolk cellularization and processing that are entirely different from the avian pattern. In birds, a vascular "yolk sac" surrounds and digests the liquid yolk. In contrast, in corn snakes, the yolk material is converted into vascularized cords of yolk-filled cells. In this study, we used stereomicroscopy, histology, and scanning electron microscopy to analyze this unusual developmental pattern in corn snakes...
March 23, 2017: Journal of Morphology
https://www.readbyqxmd.com/read/28331810/juvenile-granulosa-cell-tumor-of-the-testicle-report-of-a-neonatal-case-with-positive-alpha-fetoprotein-immunohistochemical-staining
#6
Melissa Dundas, Mark Horowitz, Richard Sidlow
We report on a case of juvenile granulosa cell tumor of the testicle in a neonate, a rare testicular tumor in children. No genital ambiguity, anatomic abnormalities, nor sex chromosome aneuploidy was noted in this patient. In our case, despite positive staining for alpha-fetoprotein which is most consistent with yolk sac tumors, all clinical, gross anatomic, histologic, and other immunohistologic characteristics of the tumor remained consistent with the diagnosis of juvenile granulosa cell tumor. The alpha-fetoprotein positivity of the tumor remains unexplained...
May 2017: Urology Case Reports
https://www.readbyqxmd.com/read/28329712/toxicity-of-effluents-from-gasoline-stations-oil-water-separators-to-early-life-stages-of-zebrafish-danio-rerio
#7
Romulo Nepomuceno Alves, Célio Freire Mariz, Driele Ventura de Paulo, Paulo S M Carvalho
Used petroleum hydrocarbons and gasoline stations runoff are significant sources of polycyclic aromatic hydrocarbons (PAHs) to aquatic ecosystems. Samples of the final effluent of oil-water-separators were collected at gasoline stations in the metropolitan region of Recife, Brazil, before release to sewage or rainwater systems. Effluent soluble fractions (ESF) were prepared and bioassays were performed according to the Fish Embryo Toxicity Test. The test involved exposing zebrafish Danio rerio embryos to dilutions of the ESFs for 96 h, with daily examination of lethality and sublethal morphological effects integrated through the General Morphology Score (GMS), based on the achievement of developmental hallmarks...
March 8, 2017: Chemosphere
https://www.readbyqxmd.com/read/28322096/cdx2-immunostaining-in-primary-and-metastatic-germ-cell-tumours-of-the-testis
#8
Fatma Oz Atalay, Berna Aytac Vuruskan, Hakan Vuruskan
Objective To evaluate the immunohistochemical staining pattern of caudal type homeobox 2 (CDX2) protein in germ cell tumours (GCTs) of the testis. Methods This study reassessed archival tissue samples collected from patients diagnosed with primary and metastatic testicular GCTs for CDX2 immunoreactivity using standard immunohistochemical techniques. Positive nuclear immunostaining was evaluated with regard to both the staining intensity and the extent of the staining. Results Tissue sections from primary and metastatic testicular GCTs ( n = 104), germ cell neoplasia in situ (GCNis) ( n = 5) and benign testicles ( n = 15) were analysed...
December 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/28319464/the-dr-jekyll-and-mr-hyde-complexity-of-the-macrophage-response-in-disease
#9
Danielle Y F Twum, Lauren Burkard-Mandel, Scott I Abrams
Macrophages comprise a highly diverse cell population expressing a continuum of biologic activities dictated by exposure to a plethora of inflammatory cues. Moreover, in contrast to most other hematopoietic populations, macrophages can arise from multiple sites-namely, the bone marrow or yolk sac, adding to the complexity of macrophage biology during health and disease. Nonetheless, it is this very type of diversity that is indispensable for macrophages to respond effectively to pathologic insults. Most of the interest in macrophage biology has been devoted to bone marrow-derived populations, but it is now becoming clearer that tissue-resident populations, which arise from distinct hematopoietic compartments, serve critical roles in host defense, including protection against neoplastic disease...
February 13, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28306215/primary-mediastinal-and-retroperitoneal-malignant-germ-cell-tumors-in-children-and-adolescents-results-of-the-tgm95-trial-a-study-of-the-french-society-of-pediatric-oncology-soci%C3%A3-t%C3%A3-fran%C3%A3-aise-des-cancers-de-l-enfant
#10
Hélène Sudour-Bonnange, Cécile Faure-Conter, Hélène Martelli, Frederic Hameury, Brice Fresneau, Daniel Orbach, Cécile Vérité
PURPOSE: To examine the clinical presentation, treatment and results in children and adolescents with primary mediastinal (PM) and retroperitoneal (RP) germ cell tumors (GCTs). METHODS: The TGM95 trial for malignant GCTs was conducted in France between 1995 and 2005 to evaluate a strategy adapted to prognostic factors with cisplatin-based chemotherapy and surgical management. We reviewed patients with TGCTs at PM and RP sites. RESULTS: Among 239 patients, there were 16 patients with PM and 5 with RP tumors, which represent 9% of all patients, highlighting the rarity of these extragonadal locations...
March 17, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28303137/cellular-and-molecular-characterization-of-microglia-a-unique-immune-cell-population
#11
REVIEW
Carole Sousa, Knut Biber, Alessandro Michelucci
Microglia are essential for the development and function of the adult brain. Microglia arise from erythro-myeloid precursors in the yolk sac and populate the brain rudiment early during development. Unlike monocytes that are constantly renewed from bone marrow hematopoietic stem cells throughout life, resident microglia in the healthy brain persist during adulthood via constant self-renewal. Their ontogeny, together with the absence of turnover from the periphery and the singular environment of the central nervous system, make microglia a unique cell population...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28299878/igy-a-key-isotype-in-antibody-evolution
#12
Xiaoying Zhang, Rosaleen A Calvert, Brian J Sutton, Katy A Doré
Immunoglobulin Y (IgY) is central to our understanding of immunoglobulin evolution. It has links to antibodies from the ancestral IgM to the mucosal IgX and IgA, as well as to mammalian serum IgG and IgE. IgY is found in amphibians, birds and reptiles, and as their most abundant serum antibody, is orthologous to mammalian IgG. However, IgY has the same domain architecture as IgM and IgE, lacking a hinge region and comprising four heavy-chain constant domains. The relationship between IgY and the mucosal antibodies IgX and IgA is discussed herein, in particular the question of how IgA could have contributed to the emergence of IgY...
March 16, 2017: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/28296679/primary-cystic-trophoblastic-tumor-of-the-testis-a-study-of-14-cases
#13
Dibson D Gondim, Thomas M Ulbright, Liang Cheng, Muhammad T Idrees
Cystic trophoblastic tumor (CTT) has been described in postchemotherapy retroperitoneal lymph node dissections of patients with testicular germ cell tumors. Prognostically, this lesion is similar to teratoma and no further treatment is required after surgery in the absence of other components. CTT has not, however, been reported in the testis. We identified 14 CTTs in the treated (4) and untreated (9; no information for 1 patient) testes of patients 15 to 43 years old (median, 25) with mixed germ cell tumors...
March 14, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28293548/age-appropriate-functions-and-dysfunctions-of-the-neonatal-neutrophil
#14
REVIEW
Shelley Melissa Lawrence, Ross Corriden, Victor Nizet
Neonatal and adult neutrophils are distinctly different from one another due to well-defined and documented deficiencies in neonatal cells, including impaired functions, reduced concentrations of microbicidal proteins and enzymes necessary for pathogen destruction, and variances in cell surface receptors. Neutrophil maturation is clearly demonstrated throughout pregnancy from the earliest hematopoietic precursors in the yolk sac to the well-developed myeloid progenitor cells in the bone marrow around the seventh month of gestation...
2017: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/28289135/the-chromatin-modifier-satb1-regulates-cell-fate-through-fgf-signalling-in-the-early-mouse-embryo
#15
Mubeen Goolam, Magdalena Zernicka-Goetz
The separation of embryonic from extra-embryonic tissues within the inner-cell-mass (ICM) to generate the epiblast (EPI), that will form the new organism, from the primitive endoderm (PE), that will form the yolk sac, is a crucial developmental decision. Here we identify a chromatin modifier, Satb1, with a distinct role in this decision. Satb1 is differentially expressed within 16-cell-stage embryos with higher expression levels in the ICM progenitor cells. Depleting Satb1 increases the number of EPI cells at the expense of PE...
March 13, 2017: Development
https://www.readbyqxmd.com/read/28287241/rapid-quantification-of-neutral-lipids-and-triglycerides-during-zebrafish-embryogenesis
#16
Prusothman Yoganantharjah, Avinesh R Byreddy, Daniel Fraher, Munish Puri, Yann Gibert
The zebrafish is a useful vertebrate model to study lipid metabolism. Oil Red-O (ORO) staining of zebrafish embryos, though sufficient for visualizing the localization of triglycerides, was previously inadequate to quantify neutral lipid abundance. For metabolic studies, it is crucial to be able to quantify lipids during embryogenesis. Currently no cost effective, rapid and reliable method exists to quantify the deposition of neutral lipids and triglycerides. Thin layer chromatography (TLC), gas chromatography and mass spectrometry can be used to accurately measure lipid levels, but are time consuming and costly in their use...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28286798/hereditary-persistence-of-alpha-fetoprotein-is-associated-with-the-119g-a-polymorphism-in-afp-gene
#17
Neha Deshpande, Radhika Chavan, Govardhan Bale, Urmila Steffie Avanthi, Mohsin Aslam, Mohan Ramchandani, D Nageshwar Reddy, V V Ravikanth
Alpha-fetoprotein (AFP) is a glycoprotein that is produced by the liver and yolk sac during fetal development. Its levels are usually raised in malignant conditions. Hereditary persistence of AFP (HPAFP) is a rare benign condition with elevated levels of AFP. It is inherited in a dominant mode with complete penetrance and is usually not associated with any clinical disability. We report two individuals with elevated levels of AFP harboring the -119G>A polymorphism in the AFP gene. A genetic screening to rule out variants in the AFP gene is advised in cases with unexplained persistent AFP levels to avoid inappropriate treatment and surgical options...
2017: ACG Case Reports Journal
https://www.readbyqxmd.com/read/28272478/erythro-myeloid-progenitors-can-differentiate-from-endothelial-cells-and-modulate-embryonic-vascular-remodeling
#18
Bahar Kasaai, Vincenza Caolo, Hanna M Peacock, Stephanie Lehoux, Elisa Gomez-Perdiguero, Aernout Luttun, Elizabeth A V Jones
Erythro-myeloid progenitors (EMPs) were recently described to arise from the yolk sac endothelium, just prior to vascular remodeling, and are the source of adult/post-natal tissue resident macrophages. Questions remain, however, concerning whether EMPs differentiate directly from the endothelium or merely pass through. We provide the first evidence in vivo that EMPs can emerge directly from endothelial cells (ECs) and demonstrate a role for these cells in vascular development. We find that EMPs express most EC markers but late EMPs and EMP-derived cells do not take up acetylated low-density lipoprotein (AcLDL), as ECs do...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28266909/exposure-to-1-2-propanediol-impacts-early-development-of-zebrafish-danio-rerio-and-induces-hyperactivity
#19
Andrey Massarsky, Ayham Abdel, Lilah Glazer, Edward D Levin, Richard T Di Giulio
The use of electronic cigarettes (e-cigarettes) is increasing as an alternative to tobacco burning cigarettes; however, their safety remains to be fully determined. The long-term effects of e-cigarettes are unknown, including the effects of maternal e-cigarette use on pre- and postnatal development. Additional research on the safety of e-cigarettes is needed. Especially useful would be information from high- and moderate-throughput economic model systems. This study investigates the effects of 1,2-propanediol, which was identified as the main component of e-cigarette liquid, on early development of zebrafish (an in vivo high-throughput model system that was recently proposed for the study of tobacco cigarette and e-cigarette toxicity)...
March 7, 2017: Zebrafish
https://www.readbyqxmd.com/read/28262537/robot-assisted-retroperitoneal-lymph-node-dissection-ra-rplnd-in-the-adolescent-population
#20
A P Glaser, D K Bowen, B W Lindgren, J J Meeks
BACKGROUND: Robot-assisted retroperitoneal lymph node dissection (RA-RPLND) has built on success and techniques of laparoscopic RPLND, with the added benefits of robotic technology. This paper demonstrates use of the da Vinci Xi(®) system for RA-RPLND in two adolescent patients. METHODS: Case #1: A 17-year-old male presented with a left testicular mass and elevated alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG). Pathology revealed a mixed non-seminomatous germ cell tumor (60% embryonal, 35% yolk sac, 5% choriocarcinoma, + lymphovascular invasion)...
February 17, 2017: Journal of Pediatric Urology
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