keyword
MENU ▼
Read by QxMD icon Read
search

Artificial

keyword
https://www.readbyqxmd.com/read/28330283/multiplex-pcr-for-simultaneous-identification-of-e-coli-o157-h7-salmonella-spp-and-l-monocytogenes-in-food
#1
Thuy Trang Nguyen, Vo Van Giau, Tuong Kha Vo
The rapid detection of pathogens in food is becoming increasingly critical for ensuring the safety of consumers, since the majority of food-borne illnesses and deaths are caused by pathogenic bacteria. Hence, rapid, sensitive, inexpensive and convenient approaches to detect food-borne pathogenic bacteria is essential in controlling food safety. In this study, a multiplex PCR assay for the rapid and simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes was established...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330201/dual-action-of-chromium-reducing-and-nitrogen-fixing-bacillus-megaterium-asnf3-for-improved-agro-rehabilitation-of-chromium-stressed-soils
#2
Sumaira Aslam, Ali Hussain, Javed Iqbal Qazi
We conducted a study for enhanced biological rehabilitation of chromium-contaminated soils using a chromium-reducing and nitrogen-fixing bacterial species (Bacillus megaterium-ASNF3). The bacterial species was isolated from a chromium-rich land area, characterized, and employed under optimum conditions for the treatment of artificially prepared chromium-rich soil. The bacterium reduced Cr(VI) up to 86 % in a 60-day trial of incubation in the soil bioreactor. The nitrogenase activity of the bacterium yielded up to 486 nmol of ethylene/mL/h after an incubation period of 40 days when it was optimally cultured in growth medium at neutral pH and 30 °C...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329940/-effectiveness-of-prevention-of-mother-to-child-hiv-transmission-in-henan-province-2002-2013
#3
Q Wang, D Y Sun, P Y Fan, Y M Ma, N Ma, H Si, N Li, Z Wang
Objective: To evaluate the effectiveness of the intervention on preventing mother to child transmission of HIV and identify the influencing factors. Methods: The data regarding the pregnant women and their infants were collected, including demographic characteristics, pregnancy and delivery, access to antiviral therapy, HIV infection status at age 18 months and survival of infants between 2002 and 2013 through follow-up, Multivariate logistic regression model were used to identify the influencing factors. Results: By the end of 2013, a total of 8 621 554 pregnant women received HIV test, among them 2 264 were infected with HIV...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329416/influence-of-continuous-rotation-or-reciprocation-of-optimum-torque-reverse-motion-on-cyclic-fatigue-resistance-of-nickel-titanium-rotary-instruments
#4
E Pedullà, G Corsentino, E Ambu, F Rovai, F Campedelli, S Rapisarda, G R La Rosa, E Rapisarda, S Grandini
AIM: To evaluate the resistance to cyclic fatigue of ProTaper Next (PTN; Dentsply Sirona, Ballaigues, Switzerland), Revo-S (Micro-Mega, Besançon, France), Mtwo (Sweden & Martina, Padova, Italy), Twisted Files (TF, SybronEndo, Orange, CA, USA) and EndoWave (J Morita Corporation, Osaka, Japan) used in continuous rotation or in reciprocation of Optimum Torque Reverse motion (OTR). METHODOLOGY: A total of 120 nickel-titanium files were tested. Twenty-four instruments for each brand were divided into 2 groups (n = 12) on the basis of the motion tested: continuous rotation (Group 1) or reciprocation of OTR motion (Group 2)...
March 22, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28329329/increased-transmissibility-of-leishmania-donovani-from-the-mammalian-host-to-vector-sand-flies-after-multiple-exposures-to-sand-fly-bites
#5
Joanna G Valverde, Andrea Paun, Ehud Inbar, Audrey Romano, Michael Lewis, Kashinath Ghosh, David Sacks
Background: Patients with active visceral leishmaniasis are important reservoirs in the anthroponotic transmission cycle of Leishmania donovani. The role of the blood or skin as a source of infection to sand flies remains unclear, and the possible effect of multiple exposures to fly bites on transmissibility has not been addressed. Methods: L. donovani infected hamsters were exposed to xenodiagnoses with Lutzomyia longipalpis on the same or different sites on the abdomen on two consecutive days, or by artificial feeding on the skin or blood...
March 9, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28329024/toward-developing-recombinant-gonadotropin-based-hormone-therapies-for-increasing-fertility-in-the-flatfish-senegalese-sole
#6
François Chauvigné, Judith Ollé, Wendy González, Neil Duncan, Ignacio Giménez, Joan Cerdà
Captive flatfishes, such as the Senegalese sole, typically produce very low volumes of sperm. This situation is particularly prevalent in the first generation (F1) of reared sole males, which limits the development of artificial fertilization methods and the implementation of selective breeding programs. In this study, we investigated whether combined treatments with homologous recombinant follicle-stimulating (rFsh) and luteinizing (rLh) hormones, produced in a mammalian host system, could stimulate spermatogenesis and enhance sperm production in Senegalese sole F1 males...
2017: PloS One
https://www.readbyqxmd.com/read/28328998/combinatorial-selection-for-replicable-rna-by-q%C3%AE-replicase-while-maintaining-encoded-gene-function
#7
Mio Yumura, Natsuko Yamamoto, Katsushi Yokoyama, Hirotada Mori, Tetsuya Yomo, Norikazu Ichihashi
Construction of a complex artificial self-replication system is challenging in the field of in vitro synthetic biology. Recently, we developed a translation-coupled RNA replication system, wherein an artificial genomic RNA replicates with the Qβ RNA replicase gene encoded on itself. The challenge is to introduce additional genes into the RNA to develop a complex system that mimics natural living systems. However, most RNA sequence encoding genes are not replicable by the Qβ replicase owing to its requirement for strong secondary structures throughout the RNA sequence that are absent in most genes...
2017: PloS One
https://www.readbyqxmd.com/read/28328498/a-framework-for-patient-state-tracking-by-classifying-multiscalar-physiologic-waveform-features
#8
Benjamin Vandendriessche, Mustafa Abas, Thomas Dick, Kenneth Loparo, Frank Jacono
OBJECTIVE: State-of-the-art algorithms that quantify nonlinear dynamics in physiologic waveforms are underutilized clinically due to their esoteric nature. We present a generalizable framework for classifying multiscalar waveform features, designed for patient-state tracking directly at the bedside. METHODS: An artificial neural network classifier was designed to evaluate multiscale waveform features against a fingerprint database of multifractal synthetic time series...
March 17, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28328189/editorial-comment-artificial-urinary-sphincter-revision-for-urethral-atrophy-comparing-single-cuff-downsizing-and-tandem-cuff-placement
#9
EDITORIAL
Marcio Augusto Averbeck
No abstract text is available yet for this article.
March 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28327880/using-plastic-tips-in-artificial-feeding-of-rhipicephalus-sanguineus-sensu-lato-acari-ixodidae-females
#10
Jaqueline Rodrigues de Almeida Valim, Charles Passos Rangel, Bruna de Azevedo Baêta, Carla Carolina Dias Uzedo Ribeiro, Matheus Dias Cordeiro, Rafaella Câmara Teixeira, Patrícia Barizon Cepeda, Adivaldo Henrique da Fonseca
This study evaluated the influence of the initial weight, feeding period and temperature on weight gain and biological parameters of the non-parasitic phase of partially engorged Rhipicephalus sanguineus females that were artificially fed using plastic tips as feeding devices. The device did not alter the oviposition of the females or any other parameters evaluated. Furthermore, it was observed that the temperature of the feeding the group did not affect the weight gain and biology of ticks. This device has great potential for the development of studies on bioagent transmission because it provides higher intake of blood by ixodid ticks...
March 16, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28327742/pair-natural-orbital-and-canonical-coupled-cluster-reaction-enthalpies-involving-light-to-heavy-alkali-and-alkaline-earth-metals-the-importance-of-sub-valence-correlation
#11
Yury Minenkov, Giovanni Bistoni, Christoph Riplinger, Alexander A Auer, Frank Neese, Luigi Cavallo
In this work, we tested canonical and domain based pair natural orbital coupled cluster methods (CCSD(T) and DLPNO-CCSD(T), respectively) for a set of 32 ligand exchange and association/dissociation reaction enthalpies involving ionic complexes of Li, Be, Na, Mg, Ca, Sr, Ba and Pb(ii). Two strategies were investigated: in the former, only valence electrons were included in the correlation treatment, giving rise to the computationally very efficient FC (frozen core) approach; in the latter, all non-ECP electrons were included in the correlation treatment, giving rise to the AE (all electron) approach...
March 22, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28327493/noninvasive-ventilation-open-issues-for-nursing-research
#12
Stefano Bambi, Enrica Mati, Christian De Felippis, Alberto Lucchini
According to the current literature, Noninvasive Ventilation (NIV) is a well-recognized respiratory support technique for patients affected by Acute Respiratory Failure (ARF). As highlighted by recent meta-analysis, a tight adherence to protocols regarding patients' selection criteria, relative or absolute contraindications, plus highly skilled and experienced operators, can positively affect the NIV performance and mortality rates. Positive outcome from NIV respiratory support is dependent from: patient's clinical condition and education needs; confidence of the staff with NIV technology; choice and management of the most suitable interface available prevention of interface complications; nutritional assessment (artificial feeding if required) and conditioning of medical gas...
March 14, 2017: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/28327192/dna-double-strand-breaks-in-human-induced-pluripotent-stem-cell-reprogramming-and-long-term-in-vitro-culturing
#13
Pavel Simara, Lenka Tesarova, Daniela Rehakova, Pavel Matula, Stanislav Stejskal, Ales Hampl, Irena Koutna
BACKGROUND: Human induced pluripotent stem cells (hiPSCs) play roles in both disease modelling and regenerative medicine. It is critical that the genomic integrity of the cells remains intact and that the DNA repair systems are fully functional. In this article, we focused on the detection of DNA double-strand breaks (DSBs) by phosphorylated histone H2AX (known as γH2AX) and p53-binding protein 1 (53BP1) in three distinct lines of hiPSCs, their source cells, and one line of human embryonic stem cells (hESCs)...
March 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28326729/-study-of-human-leucine-rich-amelogenin-peptide-and-its-regulation-of-mineralization-by-cryogenic-transmission-electron-microscopy
#14
Tian Kun, Feng Xiaoyun, Du Qin, Liao Chuhang, Ren Xiaohua
OBJECTIVE: Recombinant human leucine-rich amelogenin peptide (LRAP) was studied by cryogenic transmission electron microscopy (TEM); evaluation focused on its self-assembly and crystal growth in vitro. METHODS: Human LRAP was recombined through prokaryotic expression vector pCold-SUMO and transformed into Escherichia coli BL21plys to acquire purified proteins. Cryogen TEM recorded assembly and self-assembling of LRAP from pH 3.5 to pH 8.0, and the hydroxyapatite crystal growth in the mixture of LRAP protein solution and artificial saliva was observed using TEM and selected area electron diffraction...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28326656/skin-cancer-diagnosed-by-using-artificial-intelligence-on-clinical-images
#15
Isaäc van der Waal
In a recent Research Letter in Nature an automated classification of a few selected skin lesions has been published, using a deep convolutional neural network (CNN) (Esteva et al, 2017). Convolutional neural network is an important innovation in the field of computer vision. A popular use is for image processing, e.g. applied in face recognition. In the reported study CNN has been applied to a dataset of almost 130,000 clinical images, including some 3,000 dermoscopic images. This article is protected by copyright...
March 22, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28326614/recombinant-expression-of-porcine-spermadhesin-awn-and-its-phospholipid-interaction-indication-for-a-novel-lipid-binding-property
#16
F Schröter, K Müller, P Müller, E Krause, B C Braun
AWN is a porcine (Sus scrofa domestica) seminal plasma protein and has been linked to a variety of processes related to fertilization. To acquire the protein in sufficient amount and purity for functional studies, we established its recombinant expression in E. coli and a three-step purification protocol based on different chromatographies. The test for AWN-phospholipid interaction revealed phosphatidic acid and cardiolipin as potential binding partners. As phosphatidic acid is surmised to play a role in cation-induced membrane destabilization and fusion events, we propose a membrane protective function of the presented binding affinity...
March 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28326558/hemocd-as-a-totally-synthetic-artificial-oxygen-carrier-improvements-in-the-synthesis-and-o2-co-discrimination
#17
Hiroaki Kitagishi, Qiyue Mao, Naoya Kitamura, Takahiro Kita
HemoCD, which is composed of an iron(II)porphyrin such as 5,10,15,20-tetrakis(4-sulfonatophenyl)porphinatoiron(II) (Fe(II)TPPS) and a cyclodextrin (CD) dimer having a pyridine linker, represents a synthetic hemoglobin (Hb) model compound that exhibits reversible oxygen (O2 ) binding ability in aqueous solution at an ambient temperature. Therefore, hemoCD has the potential to be used as a totally synthetic artificial oxygen carrier. In this article, we describe the improvements of hemoCD related to its synthesis and O2 /CO selectivity...
March 22, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28326506/influence-of-calcareous-soil-on-cry3bb1-expression-and-efficacy-in-the-field
#18
David S Wangila, Arnubio Valencia J, Haichuan Wang, Blair D Siegfried, Lance J Meinke
Greater than expected injury by western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to Cry3Bb1 expressing maize hybrids (Zea mays L.) has been reported in southwestern Nebraska. Affected areas of some fields are often associated with high pH calcareous soils where maize growth is poor and iron chlorosis is common. As part of a comprehensive study to understand potential causes of unexpected injury, experiments were conducted during 2013 and 2014 to ascertain whether the calcareous soil conditions and associated poor maize growth negatively affect the expression of Cry3Bb1...
March 22, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28326483/assessment-of-sperm-quality-in-palaemonid-prawns-using-comet-assay-methodological-optimization
#19
Alexandre Erraud, Marc Bonnard, Aurélie Duflot, Alain Geffard, Jean-Michel Danger, Joëlle Forget-Leray, Benoît Xuereb
The aim of this study was to adapt the Comet assay in spermatozoa of the marine prawn Palaemon serratus to use it as a marker of sperm quality. Indeed, due to the characteristics of their spermatozoa, the measurement of DNA integrity is one of the few markers which can be transferred to crustaceans to assess the quality of their semen. In the first step, the methods of collecting and maintaining spermatozoa were optimized. Cell survival was estimated during kinetics of preservation (i.e. 1, 2, 4 and 8 h) in various suspension media to define artificial seawater (ASW) as optimal...
March 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28326461/toxicity-and-detoxification-mechanism-of-black-pepper-and-its-major-constituent-in-controlling-rhynchophorus-ferrugineus-olivier-curculionidae-coleoptera
#20
A Hussain, M Rizwan-Ul-Haq, H Al-Ayedh, A M Aljabr
The survival, feeding response, and detoxification mechanism of Rhynchophorus ferrugineus Olivier, a key pest responsible for destruction of date palm, was examined with different extracts of Piper nigrum and its major constituent (piperine) identified by GC-MS. In the present study, toxicity of different extracts of black pepper was evaluated by incorporating diffferent doses of extracts into the artificial diet of red palm weevil larvae. All extracts showed dose-dependent insecticidal activity to the tested eighth-instar red palm weevil larvae...
March 21, 2017: Neotropical Entomology
keyword
keyword
53482
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"