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https://www.readbyqxmd.com/read/27907198/a-molecular-approach-applied-to-enteroviruses-surveillance-in-northern-taiwan-2008-2012
#1
Wan-Yu Chung, Pai-Shan Chiang, Shu-Ting Luo, Tzou-Yien Lin, Kuo-Chien Tsao, Min-Shi Lee
Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1-CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Throat swabs were collected from 431 pediatric patients and 208(48%) and 250(58%) were tested positive by the VI-IFA and VP1-CODEHOP tests, respectively...
2016: PloS One
https://www.readbyqxmd.com/read/27902385/motor-coordination-and-balance-measurements-reveal-differential-pathogenicity-of-currently-spreading-ev71-strains-in-human-scarb2-transgenic-mice
#2
Mei-Feng Chen, Shin-Ru Shih
Enterovirus 71 (EV71) has caused large-scale epidemics with neurological complications in Asia Pacific region. The C4a and B5 strains are the two major genotypes circulalted in many coutries recently. This study provides a new protocol, a motor coordination task, to assess the differential pathogenicity of C4a and B5 strains in human SCARB2 transgenic mice. We found that the pathogenicity of C4a viruses was more severe than that of B5 viruses. Moreover, we discovered that an increased level of monocyte chemoattractant protein-1 is positively correlated with severely deficient motor function...
October 20, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27890633/comparison-analysis-of-micrornas-in-response-to-ev71-and-ca16-infection-in-human-bronchial-epithelial-cells-by-high-throughput-sequencing-to-reveal-differential-infective-mechanisms
#3
Yajie Hu, Jie Song, Longding Liu, Jing Li, Beibei Tang, Ying Zhang, Jingjing Wang, Lichun Wang, Shengtao Fan, Ming Feng, Qihan Li
Hand, foot, and mouth disease (HFMD) mainly caused by Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) infections which presented significantly different clinical manifestations. Nevertheless, the factors underlying these differences remain unclear. Recently, the functions of microRNAs (miRNAs) in pathogen-host interactions have been highlighted. Here, we performed comprehensive miRNA profiling in EV71- and CA16-infected human bronchial epithelial (16HBE) cells at multiple time points using high-throughput sequencing...
November 24, 2016: Virus Research
https://www.readbyqxmd.com/read/27875274/sirt1-inhibits-ev71-genome-replication-and-rna-translation-by-interfering-viral-polymerase-and-5-utr-rna
#4
Yang Han, Lvyin Wang, Jin Cui, Yu Song, Zhen Luo, Junbo Chen, Ying Xiong, Qi Zhang, Fang Liu, Wenzhe Ho, Yingle Liu, Kailang Wu, Jianguo Wu
Enterovirus 71 (EV71) possesses a single-stranded positive RNA genome that contains a single open reading frame (ORF) flanked by a 5'untranslated region (5'UTR) and a polyadenylated 3'UTR. Here, we demonstrated that EV71 activates the production of silent mating type information regulation 2 homolog 1 (SIRT1), a histone deacetylase (HDAC). EV71 further stimulates SIRT1 sumoylation and deacetylase activity, and enhances SIRT1 translocation from nuclear to cytoplasm. More interestingly, activated SIRT1 subsequently binds with EV71 3D protein (a viral RNA-dependent RNA polymerase, RdRp) to repress the acetylation and RdRp activity of 3D, resulting in the attenuation of viral genome replication...
November 14, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27873071/development-of-a-fluorescence-resonance-energy-transfer-based-intracellular-assay-to-identify-novel-enterovirus-71-antivirals
#5
Wen-Wen Lu, Fang-Yen Kung, Po-An Deng, Yu-Chu Lin, Cheng-Wen Lin, Szu-Hao Kung
Enterovirus 71 (EV71) is considered one of the most virulent pathogens in the family Picornaviridae. However, there have been no effective treatments for the severe complications caused by EV71. Development of new drugs against targets that are essential for viral replication often requires screening large collections of compounds, for which a high-throughput screening platform is needed. In this study, a drug-screening platform was developed based on a genetically engineered cell line that displays fluorescence resonance energy transfer (FRET) and shows a real-time and quantifiable impairment of FRET upon EV71 infection...
November 21, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27859744/hand-foot-and-mouth-disease-an-overview-of-clinical-features-in-adult-patients
#6
I Neri, M A Chessa, A Virdi, A Patrizi
Hand, foot and mouth disease (HFMD) mainly affects children aged <5 years and is caused by several serotypes of enteroviruses, more frequently Enterovirus 71 (EV71) and Coxsackie A16 (CA16)(1) . The disease presents with vesicles in the mouth, hands, feet and buttocks. Only 1% of infected adults develop clinical manifestations, probably as a result of immunological memory(1). Nevertheless, in the atypical HFMD caused by Coxsackie virus A6 (CVA6) the involvement of immunocompetent adults has been recently described(2-6) ...
November 11, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/27849036/enterovirus-71-infection-of-motor-neuron-like-nsc-34-cells-undergoes-a-non-lytic-exit-pathway
#7
Issac Horng Khit Too, Huimin Yeo, October Michael Sessions, Benedict Yan, Eshele Anak Libau, Josephine L C Howe, Ze Qin Lim, Shalini Suku-Maran, Wei-Yi Ong, Kaw Bing Chua, Boon Seng Wong, Vincent T K Chow, Sylvie Alonso
Enterovirus 71 (EV71) causing Hand, Foot and Mouth Disease, is regarded as the most important neurotropic virus worldwide. EV71 is believed to replicate in muscles and infect motor neurons to reach the central nervous system (CNS). To further investigate the mechanisms involved, we have employed the motor neuron cell line NSC-34. NSC-34 cells were permissive to EV71 and virus production yields were strain-dependent with differential efficacy at the entry, replication and egress steps. Furthermore, unlike all the other cell lines previously reported, EV71-infected NSC-34 cells neither displayed cytopathic effect nor underwent apoptosis...
November 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27848175/glucocorticoids-prevent-enterovirus-71-capsid-protein-vp1-induced-calreticulin-surface-exposure-by-alleviating-neuronal-er-stress
#8
Dan-Dan Hu, Jian-Ning Mai, Li-Ya He, Pei-Qing Li, Wen-Xiong Chen, Jian-Jiang Yan, Wei-Dong Zhu, Li Deng, Dan Wei, Di-Hui Liu, Si-Da Yang, Zhi-Bin Yao
Severe hand-foot-and-mouth disease (HFMD) caused by Enterovirus 71 (EV71) always accompanies with inflammation and neuronal damage in the central nervous system (CNS). During neuronal injuries, cell surface-exposed calreticulin (Ecto-CRT) is an important mediator for primary phagocytosis of viable neurons by microglia. Our data confirmed that brainstem neurons underwent neuronophagia by glia in EV71-induced death cases of HFMD. EV71 capsid proteins VP1, VP2, VP3, or VP4 did not induce apoptosis of brainstem neurons...
November 15, 2016: Neurotoxicity Research
https://www.readbyqxmd.com/read/27847364/enterovirus-71-3c-promotes-apoptosis-through-cleavage-of-pinx1-a-telomere-binding-protein
#9
Jing Li, Yunfang Yao, Yu Chen, Xiao Xu, Yongquan Lin, Zhilong Yang, Wentao Qiao, Juan Tan
: Enterovirus 71 (EV71) is an emerging pathogen causing hand, foot and mouth disease (HFMD) and fatal neurological diseases in infants and young children due to their under-developed immune-competence. EV71 infection can induce cellular apoptosis through a variety of pathways that promotes EV71 release. The viral protease 3C plays an important role in EV71-induced apoptosis. However, the molecular mechanism responsible for 3C-triggered apoptosis remains elusive. Here, we found that EV71 3C directly interacted with PinX1, a telomere binding protein...
November 9, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27843944/systematic-identification-and-bioinformatic-analysis-of-micrornas-in-response-to-infections-of-coxsackievirus-a16-and-enterovirus-71
#10
Zheng Zhu, Yuhua Qi, Huan Fan, Lunbiao Cui, Zhiyang Shi
Hand, foot, and mouth disease (HFMD), mainly caused by coxsackievirus A16 (CVA16) and enterovirus 71 (EV71) infections, remains a serious public health issue with thousands of newly diagnostic cases each year since 2008 in China. The mechanisms underlying viral infection, however, are elusive to date. In the present study, we systematically investigated the host cellular microRNA (miRNA) expression patterns in response to CVA16 and EV71 infections. Through microarray examination, 27 miRNAs (15 upregulated and 12 downregulated) were found to be coassociated with the replication process of two viruses, while the expression levels of 15 and 5 miRNAs were significantly changed in CVA16- and EV71-infected cells, respectively...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27833060/enterovirus-71-genotype-c-isolated-in-peru-between-2006-and-2009
#11
Jose L Huaman, Gladys Carrion, Julia S Ampuero, Victor Ocaña, V Alberto Laguna-Torres, Robert D Hontz
Enterovirus-71 (EV71) was first isolated in California, United States in 1969, belongs to the genus Enterovirus, family Picornaviridae. Although infection normally causes mild, often undiagnosed illness, it can cause central nervous system infections that could turn fatal. Based on VP1 gene analysis, EV71 has been classified into six separate genotypes. Although the molecular epidemiology of EV71 has been well described via studies originating from Asia and Europe, it is mostly unknown in South America. From our study, four EV71 isolates from Peru were characterized using phylogenetic methods to determine their relationship with known reference strains...
November 3, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27826325/enterovirus-71-infection-and-neurological-complications
#12
REVIEW
Kyung Yeon Lee
Since the outbreak of the enterovirus 71 (EV71) infection in Malaysia in 1997, large epidemics of EV71 have occurred in the Asia-Pacific region. Many children and infants have died from serious neurological complications during these epidemics, and EV71 infection has become a serious public health problem in these areas. EV71 infection causes hand, foot and mouth disease (HFMD) in children, and usually resolves spontaneously. However, EV71 occasionally involves the central nervous system (CNS), and induces diverse neurological complications such as brainstem encephalitis, aseptic meningitis, and acute flaccid paralysis...
October 2016: Korean Journal of Pediatrics
https://www.readbyqxmd.com/read/27818360/n-terminal-pro-b-type-natriuretic-peptide-for-the-prognostic-prediction-of-severe-enterovirus-71-associated-hand-foot-and-mouth-disease
#13
Jun Qiu, Xiulan Lu, Pingping Liu, Xinping Zhang, Chao Zuo, Zhenghui Xiao
OBJECTIVE: The aim of this study was to determine whether N-terminal pro-B-type natriuretic peptide (NT-proBNP) can predict impending brainstem encephalitis, pulmonary edema, pulmonary hemorrhage, cardiopulmonary failure, and death in children with severe enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD). METHODS: Plasma NT-proBNP levels of 282 children with severe EV71-associated HFMD were measured. RESULTS: NT-proBNP levels were significantly higher in patients with elevated blood glucose (>7...
November 3, 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27817774/-expression-of-vasoactive-intestinal-peptide-in-peripheral-blood-of-children-with-hand-foot-and-mouth-disease
#14
Jin-Song Ren, Hao-Miao Sun, Lei Zhang, Jing-De Lin, Cheng Wen, Dai-Hua Fang
OBJECTIVE: To investigate the expression of vasoactive intestinal peptide (VIP) in peripheral blood of children with hand, foot and mouth disease and its significance. METHODS: According to the condition of the disease, 86 children with hand, foot and mouth disease were classified into phase 1 group (19 children) and phase 2 group (67 children). ELISA was used to measure the concentrations of plasma VIP, interferon-γ (IFN-γ), and interleukin-4 (IL-4) in peripheral blood...
November 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/27816018/sequence-analysis-of-the-first-c2-subgenogroup-strain-of-enterovirus-71-isolated-in-korea
#15
Jung-Ah Lee, Young Sil Yoon, Ji-Yeon Hyeon, Jung Sik Yoo, Sang-Won Lee, June-Woo Lee, Sang-Won Lee
BACKGROUND: Enterovirus 71 (EV71) is an important causative agent of hand-foot-and-mouth disease with severe neurological complications, which may lead to death in children. Large outbreaks caused by EV71 have frequently occurred in Asia-Pacific region. OBJECTIVES: In Korea, the outbreaks have been caused by EV71 subgenogroups C3, and C4. Only genogroup C, especially subgenogroup C1, C3, C4, and C5, has been detected by the national enterovirus surveillance system in Korea...
October 22, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27798639/new-findings-classification-and-long-term-follow-up-study-based-on-mri-characterization-of-brainstem-encephalitis-induced-by-enterovirus-71
#16
Hongwu Zeng, Feiqiu Wen, Wenxian Huang, Yungen Gan, Weibin Zeng, Ranran Chen, Yanxia He, Yonker Wang, Zaiyi Liu, Changhong Liang, Kelvin K L Wong
BACKGROUND: To report the diversity of MRI features of brainstem encephalitis (BE) induced by Enterovirus 71. This is supported by implementation and testing of our new classification scheme in order to improve the diagnostic level on this specific disease. METHODS: Neuroimaging of 91 pediatric patients who got EV71 related BE were hospitalized between March, 2010 to October, 2012, were analyzed retrospectively. All patients underwent pre- and post-contrast MRI scan...
2016: PloS One
https://www.readbyqxmd.com/read/27793685/transcutaneous-immunization-via-rapidly-dissolvable-microneedles-protects-against-hand-foot-and-mouth-disease-caused-by-enterovirus-71
#17
Zhuangzhi Zhu, Xiaohua Ye, Zhiqiang Ku, Qingwei Liu, Chaoyun Shen, Huafei Luo, Hansen Luan, Chenghao Zhang, Shaoqiong Tian, CheeYen Lim, Zhong Huang, Hao Wang
Recent large outbreaks of hand-foot-and-mouth disease (HFMD) have seriously affected the health of young children. Enterovirus 71 (EV71) is the main causative agent of HFMD. Herein, for the first time, rapidly dissolvable microneedles (MNs) loaded with EV71 virus-like particles (VLPs) were evaluated whether they could induce robust immune responses that confer protection against EV71 infection. The characteristics of prepared MNs including hygroscopy, mechanical strength, insertion capacity, dissolution profile, skin irritation and storage stability were comprehensively assessed...
October 25, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27780225/additive-promotion-of-viral-internal-ribosome-entry-site-mediated-translation-by-far-upstream-element-binding-protein-1-and-an-enterovirus-71-induced-cleavage-product
#18
Chuan-Tien Hung, Yu-An Kung, Mei-Ling Li, Gary Brewer, Kuo-Ming Lee, Shih-Tung Liu, Shin-Ru Shih
The 5' untranslated region (5' UTR) of the enterovirus 71 (EV71) RNA genome contains an internal ribosome entry site (IRES) that is indispensable for viral protein translation. Due to the limited coding capacity of their RNA genomes, EV71 and other picornaviruses typically recruit host factors, known as IRES trans-acting factors (ITAFs), to mediate IRES-dependent translation. Here, we show that EV71 viral proteinase 2A is capable of cleaving far upstream element-binding protein 1 (FBP1), a positive ITAF that directly binds to the EV71 5' UTR linker region to promote viral IRES-driven translation...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27776325/identification-and-biochemical-characterization-of-dc07090-as-a-novel-potent-small-molecule-inhibitor-against-human-enterovirus-71-3c-protease-by-structure-based-virtual-screening
#19
Guang-Hui Ma, Yan Ye, Dan Zhang, Xin Xu, Pei Si, Jian-Long Peng, Yong-Long Xiao, Rui-Yuan Cao, Yu-Ling Yin, Jing Chen, Lin-Xiang Zhao, Yu Zhou, Wu Zhong, Hong Liu, Xiao-Min Luo, Li-Li Chen, Xu Shen
Hand, foot and mouth disease (HFMD) is a serious, highly contagious disease. HFMD caused by Enterovirus 71 (EV71), results in severe complications and even death. The pivotal role of EV71 3C(pro) in the viral life cycle makes it an attractive target for drug discovery and development to treat HFMD. In this study, we identified novel EV71 3C(pro) inhibitors by docking-based virtual screening. Totally 50 compounds were selected to test their inhibitory activity against EV71 3C(pro). The best inhibitor DC07090 exhibited the inhibition potency with an IC50 value of 21...
October 11, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27771182/a-novel-inactivated-enterovirus-71-vaccine-can-elicit-cross-protective-immunity-against-coxsackievirus-a16-in-mice
#20
Lisheng Yang, Yajing Liu, Shuxuan Li, Huan Zhao, Qiaona Lin, Hai Yu, Xiumin Huang, Qingbing Zheng, Tong Cheng, Ningshao Xia
Hand, foot, and mouth disease (HFMD) is a highly contagious disease that mainly affects infants and children. Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are the major pathogens of HFMD. Two EV71 vaccines were recently licensed in China and the administration of the EV71 vaccines is believed to significantly reduce the number of HFMD-related severe or fatal cases. However, a monovalent EV71 vaccine cannot cross-protect against CA16 infection, this may result in that it cannot effectively control the overall HFMD epidemic...
October 19, 2016: Vaccine
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