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https://www.readbyqxmd.com/read/28914420/labdane-type-diterpenoids-from-vitex-limonifolia-and-their-antivirus-activities
#1
Ninh Khac Ban, Nguyen Thi Kim Thoa, Tran My Linh, Do Thi Trang, Phan Van Kiem, Nguyen Xuan Nhiem, Bui Huu Tai, Chau Van Minh, Jae-Hyoung Song, Hyun-Jeong Ko, Seung Hyun Kim
Phytochemical investigation of the methanol extract of Vitex limonifolia leaves led to the isolation of three new labdane-type diterpenoids, vitexlimolides A-C (1-3) and eight known compounds, 5,4'-dihydroxy-3,7-dimethoxyflavone (4), vitecetin (5), 5,4'-dihydroxy-7,3'-dimethoxyflavone (6), verrucosin (7), 2α, 3α-dihydroxy-urs-12-en-28-oic acid (8), euscaphlic acid (9), 18,19-seco, 2α, 3α-dihydroxy-19-oxo-urs-11,13(18)-dien-28-oic acid (10), and maslinic acid (11). Their chemical structures were elucidated by physical and chemical methods...
September 15, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28910400/293texosome-mediated-mir-146a-transfer-suppresses-type-i-interferon-response-and-facilitates-ev71-infection
#2
Yuxuan Fu, Li Zhang, Fang Zhang, Ting Tang, Qi Zhou, Chunhong Feng, Yu Jin, Zhiwei Wu
Exosomes can transfer genetic materials between cells. Their roles in viral infections are beginning to be appreciated. Researches have shown that exosomes released from virus-infected cells contain a variety of viral and host cellular factors that are able to modulate recipient's cellular response and result in productive infection of the recipient host. Here, we showed that EV71 infection resulted in upregulated exosome secretion and differential packaging of the viral genomic RNA and miR-146a into exosomes...
September 14, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28900642/a-mutation-resistant-deoxyribozyme-or-gate-for-highly-selective-detection-of-viral-nucleic-acids
#3
Ola Kamar, Sin-Cih Sun, Chieh-Hua Lin, Wan-Yu Chung, Min-Shi Lee, Yu-Chieh Liao, Dmitry M Kolpashchikov, Min-Chieh Chuang
Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases...
September 13, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28892475/cost-effectiveness-of-a-national-enterovirus-71-vaccination-program-in-china
#4
Wenjun Wang, Jianwen Song, Jingjing Wang, Yaping Li, Huiling Deng, Mei Li, Ning Gao, Song Zhai, Shuangsuo Dang, Xin Zhang, Xiaoli Jia
BACKGROUND AND AIMS: Enterovirus 71 (EV71) has caused great morbidity, mortality, and use of health service in children younger than five years in China. Vaccines against EV71 have been proved effective and safe by recent phase 3 trials and are now available in China. The purpose of this study was to evaluate the health impact and cost-effectiveness of a national EV71 vaccination program in China. METHODS: Using Microsoft Excel, a decision model was built to calculate the net clinical and economic outcomes of EV71 vaccination compared with no EV71 vaccination in a birth cohort of 1,000,000 Chinese children followed for five years...
September 11, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28882666/clinical-features-for-89-deaths-of-hand-foot-and-mouth-disease-in-guangxi-china-2014
#5
Wei Lin, Ying Su, Min Jiang, Jing Liu, Yan-Yan Zhang, Guang-Min Nong
OBJECTIVES: The aim of this study is to summarize the risk factors of severe Hand, foot and mouth disease (HFMD) and explore the clinical characteristics of pulmonary edema (PE) and non-PE in the expired patients with HFMD. METHODS: We identified 89 HFMD deaths which were separated into the PE group or non-PE group. Next, patients were divided based on their initial admission to hospitals as stage 1, 2, 3, or 4; at this point, their clinical manifestations were compared...
September 4, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28871120/development-of-a-luciferase-based-biosensor-to-assess-enterovirus-71-3c-protease-activity-in-living-cells
#6
Yuan Zhang, Xianliang Ke, Caishang Zheng, Yan Liu, Li Xie, Zhenhua Zheng, Hanzhong Wang
Enterovirus 71 (EV71) is a major pathogen of hand, foot, and mouth disease (HFMD). To date, no antiviral drug has been approved to treat EV71 infection. Due to the essential role that EV71 3 C protease (3C(pro)) plays in the viral life cycle, it is generally considered as a highly appealing target for antiviral drug development. In this study, we present a transgene-encoded biosensor that can accurately, sensitively and quantitatively report the proteolytic activity of EV71 3C(pro). This biosensor is based on the catalyzed activity of a pro-interleukin (IL)-1β-enterovirus 3C(pro) cleavage site-Gaussia Luciferase (GLuc) fusion protein that we named i-3CS-GLuc...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28868926/a-novel-chloro-substituted-pentenamide-from-the-fruiting-bodies-of-amanita-virgineoides
#7
Liang-Yan Liu, Ji-Kai Liu, Wen-Han Su, Tao Peng
One unusual chloro-substituted pentenamide, (3R)-4-chloro-3-hydroxy-4-pentenamide (1), together with 11 known compounds (2-12) were isolated from the fruiting bodies of Amanita virgineoides. The structure of 1 including the absolute configuration was characterized by extensive spectroscopic analyses and quantum calculation. Compound 1 displayed no obvious activity against herpes simplex virus (HSV), human enterovirus 71 (EV71) or coxsackievirus B3 (CVB3).
September 4, 2017: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/28867629/a-nanobiosensing-method-based-on-force-measurement-of-antibody-antigen-interaction-for-direct-detection-of-enterovirus-71-by-the-chemically-modified-atomic-force-microscopic-probe
#8
Chung-Hung Hong, Chung-Fan Hsieh, Chi-Shin Tseng, Wei-Chih Huang, Cheng-Yan Guo, Shiming Lin, Si-Chen Lee
Hand, Foot and mouth disease (HFMD) is a common disease with high infectivity for children, and enterovirus 71 (EV71) is one of the main pathogens to cause the type of illness. Therefore, the aim of this study was to propose a rapid and effective technique for detecting EV71 directly based on the mechanism of biological intermolecular force by using atomic force microscopy (AFM). At first, we coated EV71 particles on the mica surface and made the EV71 antibodies (anti-EV71) fixed on the AFM tip by means of several chemical procedures...
September 1, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28866699/a-sudden-paediatric-death-due-to-hand-foot-and-mouth-disease-the-importance-of-vigilance
#9
R Rahimi, E Omar, T S Tuan Soh, S F A Mohd Nawi, S Md Noor
BACKGROUND: Hand, foot and mouth disease (HFMD) is caused by enteroviruses such as Coxsackie virus A16 (CVA16) and Enterovirus 71 (EV71). The diagnostic hallmarks are oral ulcers and maculo-papular or vesicular rash on the hands and feet. Severe form of this disease can lead to death due to neurological and cardiopulmonary complications. This case report aims to describe a fatal case of HFMD with minimal oral and skin manifestations. CASE REPORT: A four-year-old girl was brought to a hospital after suddenly becoming unresponsive at home...
August 2017: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/28858766/asymmetric-synthesis-of-novel-triazole-derivatives-and-their-in%C3%A2-vitro-antiviral-activity-and-mechanism-of-action
#10
Xiufang Cao, Wenda Wang, Shuangshuang Wang, Longzhu Bao
In this study, forty-four chiral triazole derivatives have been prepared via asymmetric synthesis, and which has been successfully characterized by typical spectroscopic techniques including (1)H NMR, (13)C NMR, EI-MS, elemental analysis and optical rotations. Their in vitro antiviral activities against EV71 and CVB3 were fully investigated in cell-based assays. It was observed that 13 synthetic triazole derivatives inhibited the CPE of EV71 on RD cells, with EC50S in the 5.3-15.9 μg/ml range and corresponding SIs of 4...
August 26, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28854257/hrs-plays-an-important-role-for-tlr7-signaling-to-orchestrate-inflammation-and-innate-immunity-upon-ev71-infection
#11
Zhen Luo, Maolin Ge, Junbo Chen, Qibin Geng, Mingfu Tian, Zhi Qiao, Lan Bai, Qi Zhang, Chengliang Zhu, Ying Xiong, Kailang Wu, Fang Liu, Yingle Liu, Jianguo Wu
Enterovirus 71 (EV71) is an RNA virus that causes hand-foot-mouth disease (HFMD), and even fatal encephalitis in children. Although EV71 pathogenesis remains largely obscure, host immune responses may play important roles in the development of diseases. Recognition of pathogens mediated by Toll-like receptors (TLRs) induces host immune and inflammatory responses. Intracellular TLRs must traffic from the endoplasmic reticulum (ER) to the endolysosomal network from where they initiate complete signaling, leading to inflammatory response...
August 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28843383/il-10-592-polymorphism-is-associated-with-il-10-expression-and-severity-of-enterovirus-71-infection-in-chinese-children
#12
Na Zhao, Hui-Lan Chen, Zhen-Zhen Chen, Jing Li, Zong-Bo Chen
BACKGROUND: Enterovirus 71 (EV71) infection results in some severe complications with high mortality and disability in Hand, Foot and Mouth Disease (HFMD) in children. Recent studies have shown that cytokine genetic predispositions have associations with both the development of EV71 infection and severity of HFMD. OBJECTIVE: This study was designed to investigate whether the IL-10-592 polymorphism is associated with IL-10 levels and disease severity in Chinese children with EV71 infection...
August 19, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28835799/discovery-of-potent-ev71-capsid-inhibitors-for-treatment-of-hfmd
#13
Peng Li, Jun Yu, Fei Hao, Haiying He, Xuyang Shi, Jiao Hu, Li Wang, Chunyan Du, Xiao Zhang, Ya Sun, Fusen Lin, Zhengxian Gu, Deming Xu, Xinsheng Chen, Liang Shen, Guoping Hu, Jian Li, Shuhui Chen, Wei Xiao, Zhenzhong Wang, Qingming Guo, Xiujuan Chang, Xuyang Tian, Tianwei Lin
Enterovirus 71 (EV71) is a major causative agent of hand, foot, and mouth disease (HFMD), which can spread its infections to the central nervous and other systems with severe consequences. The viral caspid protein VP1 is a well-known target for antiviral efficacy because its occupancy by suitable compounds could stabilize the virus capsid, thus preventing uncoating of virus for RNA release. In this Letter, design, synthesis, and biological evaluation of novel anti-EV71 agents (aminopyridyl 1,2,5-thiadiazolidine 1,1-dioxides) are described...
August 10, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28832767/antiviral-activity-of-shikonin-ester-derivative-pmm-034-against-enterovirus-71-in-vitro
#14
Y Zhang, H Han, L Sun, H Qiu, H Lin, L Yu, W Zhu, J Qi, R Yang, Y Pang, X Wang, G Lu, Y Yang
Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD), particularly in infants and children below 4 years of age. Shikonin is a bioactive compound with anti-inflammatory, antiviral, and antibacterial activities derived from the roots of the Chinese medicinal herb Lithospermum erythrorhizon. This study aimed to examine the antiviral activity of PMM-034, a shikonin ester derivative, against EV71 in rhabdomyosarcoma (RD) cells. Cytotoxicity of PMM-034 on RD cells was determined using WST-1 assay...
August 17, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28828580/formulation-and-in-vitro-release-kinetics-of-mucoadhesive-blend-gels-containing-matrine-for-buccal-administration
#15
Xiaojin Chen, Jun Yan, Shuying Yu, Pingping Wang
Enterovirus 71 (EV71) is a pathogenic factor of severe hand, foot, and mouth disease (HFMD). No vaccine or specific treatment is currently available for EV71 infection. Hence, we developed a buccal mucoadhesive gel containing matrine to protect against HFMD. Mucoadhesive gels were prepared by Carbopol 974P and were combined with Carbopol 971P, sodium carboxymethyl cellulose (CMC-Na), or hydroxypropylmethy cellulose (HPMC K100M). The formulations were characterized in terms of tensile testing and continuous flow techniques for mucoadhesion...
August 21, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28756983/morphological-characteristics-of-fatal-pediatric-hand-foot-and-mouth-disease-a-clinicopathological-study-with-related-receptors-of-ev71
#16
Yong-Yao Gu, Ke Shi, Sha Yao, Xia Yang, Yu-Hui Liu, Lan Tang, Yi-Wu Dang, Gang Chen, Zhen-Bo Feng, Hong-Bo Pan
OBJECTIVE: To investigate the pathological features of fatal pediatric hand foot and mouth disease (HFMD). METHODS: The histopathological features of HFMD were first summarized from literature, and then confirmed by in-house autopsies. Furthermore, immunohistochemistry was conducted to detect the distribution and expression level of two enterovirus 71 (EV71) receptors scavenger receptor class B, member 2 (SCARB2), and P-selectin glycoprotein ligand-1 (PSGL1) in the samples of autopsies...
July 6, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28756324/a-colorimetric-and-electrochemical-immunosensor-for-point-of-care-detection-of-enterovirus-71
#17
Yuan-Hui Hou, Jia-Jia Wang, Yong-Zhong Jiang, Cheng Lv, Li Xia, Shao-Li Hong, Miao Lin, Yi Lin, Zhi-Ling Zhang, Dai-Wen Pang
Point-of-care detection of human enterovirus 71 (EV71), the major pathogen that causes hand, foot, and mouth disease (HFMD) among children, is urgently needed for early diagnosis and control of related epidemics. A colorimetric and electrochemical immunosensor for point-of-care detection of EV71 has been developed based on dual-labeled magnetic nanobeads amplification. The dual-labeled magnetic nanobeads (DL-MBs) are fabricated by simultaneous immobilization of EV71 monoclonal antibody (mAb) and horseradish peroxidase (HRP) on magnetic nanobeads...
July 14, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28735328/molecular-characterization-of-enteroviruses-isolated-from-acute-flaccid-paralysis-cases-in-poland-1999-2014
#18
Magdalena Wieczorek, Arleta Krzysztoszek
Enteroviruses (EVs) are among viral pathogens that can cause acute flaccid paralysis (AFP). This study represents an overview of EVs isolated through AFP surveillance in Poland between 1999 and 2014. The presence of enteroviruses was studied in stool samples that were collected from 747 AFP cases and their asymptomatic contacts. Fifty five (6.12%) cases of AFP were associated with enterovirus isolation. Out of the 55 positive cases, 40 were associated with detection of enterovirus in patient, and 15 with detection of EV in healthy contact, without positive detection in paralytic patient...
January 2, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28732070/characterization-of-human-enterovirus71-virus-like-particles-used-for-vaccine-antigens
#19
Dandan Zhao, Bo Sun, Shiyang Sun, Bin Fu, Chuntian Liu, Dawei Liu, Yanfei Chu, Youlei Ma, Lu Bai, Yongge Wu, Yan Zhou, Weiheng Su, Ali Hou, Linjun Cai, Fei Xu, Wei Kong, Chunlai Jiang
Human enterovirus 71 (EV71) is a major causative pathogen of hand, foot and mouth disease (HFMD) and has caused outbreaks with significant mortality among young children in the Asia-Pacific region in recent years. Towards developing a vaccine for this disease, we have expressed and purified EV71 virus-like particles (VLPs), which resemble the authentic virus in appearance, capsid structure and protein sequence, from insect cells (Sf9) using a multistep chromatography process. We demonstrated intracellular localization of the VLPs in host cells by in situ immunogold detection, electron microscopy and immunofluorescence...
2017: PloS One
https://www.readbyqxmd.com/read/28724943/aim2-inflammasome-mediated-pyroptosis-in-enterovirus-a71-infected-neuronal-cells-restricts-viral-replication
#20
Thinesshwary Yogarajah, Kien Chai Ong, David Perera, Kum Thong Wong
Encephalomyelitis is a well-known complication of hand, foot, and mouth disease (HFMD) due to Enterovirus 71 (EV71) infection. Viral RNA/antigens could be detected in the central nervous system (CNS) neurons in fatal encephalomyelitis but the mechanisms of neuronal cell death is not clearly understood. We investigated the role of absent in melanoma 2 (AIM2) inflammasome in neuronal cell death, and its relationship to viral replication. Our transcriptomic analysis, RT-qPCR, Western blot, immunofluorescence and flow cytometry studies consistently showed AIM2 gene up-regulation and protein expression in EV-A71-infected SK-N-SH cells...
July 19, 2017: Scientific Reports
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