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https://www.readbyqxmd.com/read/29777102/a-virus-like-particle-based-tetravalent-vaccine-for-hand-foot-and-mouth-disease-elicits-broad-and-balanced-protective-immunity
#1
Wei Zhang, Wenlong Dai, Chao Zhang, Yu Zhou, Pei Xiong, Shuxia Wang, Xiaohua Ye, Qingwei Liu, Dongming Zhou, Zhong Huang
Hand, foot, and mouth disease (HFMD) is an infectious disease that mainly affects infants and children, causing considerable morbidity and mortality worldwide. HFMD is commonly caused by enterovirus 71 (EV71) and coxsackieviruses A16 (CVA16), A6 (CVA6), and A10 (CVA10). Formalin-inactivated EV71 vaccines are currently available in China; however, these vaccines fail to confer cross-protection against infections by other HFMD-causing enteroviruses, highlighting the necessity of developing a multivalent HFMD vaccine...
May 18, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29777028/microrna-302-cluster-downregulates-enterovirus-71-induced-innate-immune-response-by-targeting-kpna2
#2
Nanfang Peng, Xuecheng Yang, Chengliang Zhu, Li Zhou, Haisheng Yu, Mengqi Li, Yong Lin, Xueyu Wang, Qian Li, Yinglong She, Jun Wang, Qian Zhao, Mengji Lu, Ying Zhu, Shi Liu
Enterovirus 71 (EV71) induces significantly elevated levels of cytokines and chemokines, leading to local or systemic inflammation and severe complications. As shown in our previous study, microRNA (miR) 302c regulates influenza A virus-induced IFN expression by targeting NF-κB-inducing kinase. However, little is known about the role of the miR-302 cluster in EV71-mediated proinflammatory responses. In this study, we found that the miR-302 cluster controls EV71-induced cytokine expression. Further studies demonstrated that karyopherin α2 (KPNA2) is a direct target of the miR-302 cluster...
May 18, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29776754/willingness-and-influential-factors-of-parents-to-vaccinate-their-children-with-novel-inactivated-enterovirus-71-vaccines-in-guangzhou-china
#3
Tiegang Li, Hui Wang, Yin Lu, Qin Li, Chun Chen, Dahu Wang, Meixia Li, Yilan Li, Jianyun Lu, Zongqiu Chen, Yu Ma, Wenhui Liu, Mengmeng Ma, Di Wu, Jiachun Lu, Zhicong Yang
Hand, foot and mouth disease (HFMD) primarily affects children younger than 5 years of age. Recently, HFMD has ranked as the top notifiable infectious disease in China. In December 2015, China approved two novel inactivated enterovirus 71 vaccines (EV71 vaccines) for HFMD. Parents' acceptance is often essential for vaccination program success. The goal of this study was to identify willingness and influential factors to vaccinate among parents of kindergarteners in Guangzhou, China. A cross-sectional survey of face-to-face interviews was conducted from March to July 2016...
May 15, 2018: Vaccine
https://www.readbyqxmd.com/read/29767300/development-and-evaluation-of-a-rapid-recombinase-polymerase-amplification-assay-for-the-detection-of-human-enterovirus-71
#4
Dan Yin, Yanan Zhu, Kaifeng Wang, Jing Wang, Xiru Zhang, Min Han, Yaqing He, Qing Chen, Guifang Hu
Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%. In a clinical sample evaluation, this approach demonstrated sufficient clinical performance when compared with a commercial RT-qPCR diagnostic kit. Thus, the RT-RPA assay may be a promising alternative for the detection of EV71-C4...
May 16, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29765110/mast-cells-contribute-to-enterovirus-71-infection-induced-pulmonary-edema-in-neonatal-mice
#5
Yuefei Jin, Chao Zhang, Hui Wang, Guangyuan Zhou, Xiangpeng Wang, Rongguang Zhang, Shuaiyin Chen, Jingchao Ren, Lu Chen, Dejian Dang, Peng Zhang, Yuanlin Xi, Weidong Wu, Weiguo Zhang, Guangcai Duan
Enterovirus (EV) 71 infection has been widely acknowledged as the leading cause of severe hand, foot and mouth disease (HFMD), which may rapidly lead to fatal pulmonary edema. In this study, we established a mouse model for EV71 infection exhibiting high incidence of severe symptoms with pulmonary edema. Mast cells (MCs) accumulation, activation and allergic inflammation were found in the brains, lungs and skeletal muscle of mice after EV71 infection, especially in the lungs of mice. Levels of histamine, platelet-activating factor (PAF), interleukin (IL)-4, IL-5, IL-13, tumor necrosis factor-α (TNF-α), nitric oxide (NO), endocrine gland-derived vascular endothelial growth factor (EG-VEGF) and noradrenaline (NA) were increased in EV71-infected lungs...
May 15, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29755438/caspase-3-inhibition-attenuates-the-cytopathic-effects-of-ev71-infection
#6
Fengmei Song, Xiaoyan Yu, Ting Zhong, Zengyan Wang, Xiangling Meng, Zhaolong Li, Shuxia Zhang, Wenbo Huo, Xin Liu, Yahong Zhang, Wenyan Zhang, Jinghua Yu
Previous studies demonstrate that human enterovirus 71 (EV71), a primary causative agent for hand, foot, and mouth disease, activates caspase-3 through the non-structural viral 3C protein to induce host cell apoptosis; however, until now it was unclear how 3C activates caspase-3 and how caspase-3 activation affects viral production. Our results demonstrate that 3C binds caspase-8 and caspase-9 but does not directly bind caspase-3 to activate them, and that the proteolytic activity of 3C is required by the activation of caspase-8, caspase-9, and caspase-3...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29743570/enterovirus-71-infection-of-human-airway-organoids-reveals-vp1-145-as-a-viral-infectivity-determinant
#7
Sabine M G van der Sanden, Norman Sachs, Sylvie M Koekkoek, Gerrit Koen, Dasja Pajkrt, Hans Clevers, Katja C Wolthers
Human enteroviruses frequently cause severe diseases in children. Human enteroviruses are transmitted via the fecal-oral route and respiratory droplets, and primary replication occurs in the gastro-intestinal and respiratory tracts; however, how enteroviruses infect these sites is largely unknown. Human intestinal organoids have recently proven to be valuable tools for studying enterovirus-host interactions in the intestinal tract. In this study, we demonstrated the susceptibility of a newly developed human airway organoid model for enterovirus 71 (EV71) infection...
May 9, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29730555/harmine-a-small-molecule-derived-from-natural-sources-inhibits-enterovirus-71-replication-by-targeting-nf-%C3%AE%C2%BAb-pathway
#8
Deyan Chen, Xiaoyan Tian, Xue Zou, Shijie Xu, Huanru Wang, Nan Zheng, Zhiwei Wu
Enterovirus 71 (EV71) infection of young children can cause neurological manifestations, which is mainly responsible for the fatality. Although a vaccine is recently available for preventing enterovirus 71 infection, its efficacy remains to be seen. Therefore, there is a pressing need for anti-viral agents for the treatment of EV71 infection. By screening a natural compound library for inhibitory activity of EV71 replication, we identified a small molecule, harmine, that inhibited EV71 replication by targeting NF-κB signaling pathway...
May 2, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29703921/mutations-in-vp1-and-5-utr-affect-enterovirus-71-virulence
#9
Ching-Kun Chang, Shang-Rung Wu, Ying-Chin Chen, Kuen-Jin Lee, Nai-Hsiang Chung, Yi-Ju Lu, Shu-Ling Yu, Chia-Chyi Liu, Yen-Hung Chow
Enterovirus 71 (EV71) is a major cause of hand, foot and mouth disease (HFMD). The current EV71 propagating in Vero (EV-V) or sub-passaged in RD (EV-R) cells was used as a pathogen. Interestingly, EV-R exhibited differential virulence; challenging human scavenger receptor class B2-expressing (hSCARB2-Tg) mice with EV71 revealed that EV-V was more virulent than EV-R: 100% of mice that received lethal amounts of EV-V died, while all the mice that received EV-R survived. Severe pathogenesis correlated with viral burdens and proinflammatory cytokine levels were observed in EV-V-challenged mice, but controversy in EV-R-challenged mice...
April 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29702280/crosstalk-between-toll-like-receptor-3-and-notch-signaling-contributes-to-cd14-monocytes-activity-in-enterovirus-71-infected-hand-foot-and-mouth-disease
#10
Jie Hao, Peiling Li, Yunjiao Tian, Yanhua Wang, Suqing Li, Lina Wang, Shujun Li
Interaction between Toll-like receptor (TLR) and Notch signaling contributes to inflammatory response in nephropathy and fungicidal infection, however, the role of this crosstalk remains not fully elucidated in enterovirus 71 (EV71)-induced hand, foot, and mouth disease (HFMD). The aim of this study was to investigate the crosstalk between TLR and Notch in inflammatory regulation in EV71 infection. Thirty-seven EV-71-indcued HFMD (16 mild and 21 severe cases) and eleven normal control (NC) were enrolled. CD14+ monocytes were purified, and were stimulated with either TLR3/4 agonists [poly(I: C) or LPS] or Notch signaling inhibitor...
April 24, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29685021/detection-of-hand-foot-mouth-disease-and-its-spatial-temporal-epidemiological-characteristics-with-gis-platform
#11
X J Xu, Z Tang, L R Zeng, H Luo, Q Y Chen, L Li, Q Lou, X J He
The aim of this study is to investigate the main category of hand-foot-mouth (HFM) virus and analyze the distribution characteristics and susceptible population of HFM disease in China. Infants who have had HFM disease for less than 7 days were selected from the First Affiliated Hospital of Guangxi Medical University, Guangxi, China. Various specimens were collected from the infants, and EV71 and CA16 nucleic acid detections were performed using fluorescence quantitative assay. The positive results of the specimens were compared to determine the components of the pathogen...
March 2018: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29624826/site-specific-incorporation-of-chemical-fluorescence-on-live-enterovirus-71-virion-by-organometallic-palladium-reagent-to-monitor-virus-entry
#12
Zhiyong Lou, Yaxin Wang, Jingwei Liu, Lin Cao, Wenjing Wang, Yuna Sun, Zheng Yin
Imaging live virus to monitor the viral entry process is essential to understand virus-host interactions during pathogen infection. However, methods for efficient labeling of live viruses, in particular labeling non-enveloped viruses and tracing virus entry processes, remains limited. Recently, labeling using organometallic palladium reagents has provided a highly efficient and a highly selective way to bioconjugate cysteines of virus proteins. Here, the site-specific bioorthogonal labeling mediated by an organometallic palladium reagent on the surface of live enterovirus-71 (EV71) was used to visualize its entry into live cells...
April 6, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29619599/inactivated-enterovirus-71-with-poly-%C3%AE-glutamic-acid-chitosan-nano-particles-pc-nps-induces-high-cellular-and-humoral-immune-responses-in-balb-c-mice
#13
Prabuddha S Pathinayake, W A Gayan Chathuranga, Hyun-Cheol Lee, Mohammed Y E Chowdhury, Moon-Hee Sung, Jong-Soo Lee, Chul-Joong Kim
Enterovirus 71 (EV71) is the major causative agent of hand-foot-and-mouth disease (HFMD) and many neurological manifestations. Recently, this virus has become a serious concern because of consecutive epidemics in the Asia-Pacific region. However, no effective vaccine for EV71 has been discovered except two EV71 vaccines which are being used in local communities of China. To develop a safe and efficient EV71 vaccine candidate, we generated inactivated EV71 and evaluated its efficacy with γ-PGA/Chitosan nanoparticles (PC NPs), which are safe, biodegradable and effective as an adjuvant...
April 4, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29619244/antiviral-effects-of-ferric-ammonium-citrate
#14
Hongbin Wang, Zheng Li, Junling Niu, Yongfen Xu, Li Ma, Ailing Lu, Xun Wang, Zhikang Qian, Zhong Huang, Xia Jin, Qibin Leng, Jianhua Wang, Jin Zhong, Bing Sun, Guangxun Meng
Iron is an essential nutrient for cell survival and is crucial for DNA replication, mitochondrial function and erythropoiesis. However, the immunological role of iron in viral infections has not been well defined. Here we found the iron salt ferric ammonium citrate (FAC) inhibited Influenza A virus, HIV virus, Zika virus, and Enterovirus 71 (EV71) infections. Of note, both iron ion and citrate ion were required for the antiviral capability of FAC, as other iron salts and citrates did not exhibit viral inhibition...
2018: Cell Discovery
https://www.readbyqxmd.com/read/29615683/non-neutralizing-antibody-responses-against-vp1-in-enterovirus-a-b-c-and-rhinovirus-a-species-among-infants-and-children-in-shanghai
#15
Yingying Ding, Bing Rui, Caixia Gao, Menghua Xu, Lili Wang, Chunyan Zhao, Jie Bai, Jinhong Wang, Jin Xu, Wei Pan
The overall non-neutralizing antibody responses against EV infections among infants and children remain unknown. The non-neutralizing antibody responses against VP1 of EV-A species (Enterovirus 71 (EV71), Coxsackievirus A16 (CA16)), EV-B species (Coxsackievirus B3 (CB3)), EV-C species (Poliovirus 1 (PV1)) and RV-A species (Rhinovirus A N13 (RV13)) were detected and analyzed using a novel evolved immunoglobulin-binding molecule (NEIBM)-based ELISA among infants and children aged 1 day to 6 years in Shanghai...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29603282/sequence-analysis-based-characterization-and-identification-of-neurovirulence-associated-variants-of-36-ev71-strains-from-china
#16
Jun Xu, Fang Wang, Desheng Zhao, Jiang Liu, Hong Su, Baolong Wang
Enterovirus 71 (EV71) is the main pathogen of hand-foot-mouth disease (HFMD) and causes several neurological complications. As new strains of EV71 are constantly discovered, it is important to understand the genomic characteristics of the viruses and the mechanism of virulence. Herein, we isolated five strains of EV71 from HFMD patients with or without neurovirulence and sequenced their whole genomes. We then performed whole genome sequence analysis of totally 36 EV71 strains. The phylogenetic analysis of the VP1 region revealed all five isolated strains are clustered into C4a of C4 subgenotype...
March 30, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29597291/antiviral-and-inflammatory-cellular-signaling-associated-with-enterovirus-71-infection
#17
REVIEW
Yuefei Jin, Rongguang Zhang, Weidong Wu, Guangcai Duan
Enterovirus 71 (EV71) infection has become a major threat to global public health, especially in infants and young children. Epidemiological studies have indicated that EV71 infection is responsible for severe and even fatal cases of hand, foot, and mouth disease (HFMD). Accumulated evidence indicates that EV71 infection triggers a plethora of interactive signaling pathways, resulting in host immune evasion and inflammatory response. This review mainly covers the effects of EV71 infection on major antiviral and inflammatory cellular signal pathways...
March 28, 2018: Viruses
https://www.readbyqxmd.com/read/29582164/induction-of-a-high-titered-antibody-response-using-hiv-gag-ev71-vp1-based-virus-like-particles-with-the-capacity-to-protect-newborn-mice-challenged-with-a-lethal-dose-of-enterovirus-71
#18
Xi Wang, Ke Dong, Min Long, Fang Lin, Zhaowei Gao, Lin Wang, Zhe Zhang, Xi Chen, Ying Dai, Huiping Wang, Huizhong Zhang
Enterovirus 71 (EV71) is the most frequently detected causative agent in hand, foot, and mouth disease (HFMD) and is a serious threat to public health in the Asia-Pacific region. Many EV71 vaccines are under development worldwide, and although both inactivated virus vaccines and virus-like particles (VLPs) are considered to be effective, the main focus has been on inactivated EV71vaccines. There have been very few studies on EV71 VLPs. In this study, using a strategy based on HIV gag VLPs, we constructed a gag-VP1 fusion gene to generate a recombinant baculovirus expressing the EV71 structural protein VP1 together with gag, which was then used to infect TN5 cells to form VLPs...
March 27, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29572108/establishment-of-the-1st-who-international-standard-for-anti-ev71-serum-human
#19
Gillian Cooper, Qunying Mao, Laura Crawt, Yiping Wang, Thomas Dougall, Peter Rigsby, Zhenglun Liang, Miao Xu, Philip Minor, Junzhi Wang, Javier Martin
Enterovirus A71 (EV71) is the major causative agent of severe and fatal hand, foot and mouth disease. There is plenty of evidence that EV71 has circulated widely in the Western Pacific Region for the last twenty years. Vaccines against EV71 are already available or under development. A collaborative study to establish the 1st WHO International Standard for anti-EV71 serum (Human) was conducted to ensure that methods used to measure the serum neutralizing activity or antibody levels against EV71 are accurate, sensitive and reproducible...
March 20, 2018: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29549443/association-of-interleukin-17f-gene-polymorphisms-with-susceptibility-to-severe-enterovirus-71-infection-in-chinese-children
#20
Fei Li, Peipei Liu, Ya Guo, Zhenliang Han, Yedan Liu, Yuanyuan Wang, Long Song, Jianguo Cheng, Zongbo Chen
Enterovirus 71 (EV71) is a single-strand RNA virus that causes hand, foot and mouth disease (HFMD) in infants and young children, leading to neurological complications with significant morbidity and mortality. Unfortunately, the pathogenesis of EV71 infection is not well understood. In this study, we investigated the IL-17F rs1889570 and rs4715290 gene polymorphisms in a Chinese Han population. Severe cases and cases with EV71 encephalitis had a significantly higher frequency of the rs1889570 T/T genotype and T allele...
March 16, 2018: Archives of Virology
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