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https://www.readbyqxmd.com/read/27582290/effects-of-nitrite-stress-on-mrna-expression-of-antioxidant-enzymes-immune-related-genes-and-apoptosis-related-proteins-in-marsupenaeus-japonicus
#1
Jinbin Zheng, Yong Mao, Yongquan Su, Jun Wang
Nitrite accumulation in aquaculture systems is a potential risk factor that may trigger stress responses in aquatic organisms. However, the mechanisms regulating the responses of shrimp to nitrite stress remain unclear. In this study, full-length cDNA sequences of two apoptosis-related genes, caspase-3 and defender against apoptotic death (DAD-1), were cloned from Marsupenaeus japonicus for the first time, and their expression levels and tissue distribution were analyzed by quantitative real-time PCR (qRT-PCR)...
August 28, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26439413/identification-and-expression-analysis-of-a-novel-stylicin-antimicrobial-peptide-from-kuruma-shrimp-marsupenaeus-japonicus
#2
Hong-tao Liu, Jun Wang, Yong Mao, Min Liu, Su-fang Niu, Ying Qiao, Yong-quan Su, Chun-zhong Wang, Zhi-peng Zheng
Antimicrobial peptides (AMPs) are important components of the innate immune system and function as the first line of defense against invading pathogens. In current study we identified, cloned and characterized a novel stylicin AMP from Kuruma shrimp Marsupenaeus japonicus (Mj-sty). The full-length cDNA of Mj-sty was 428 bp with an open reading frame of 315 bp that encoded 104 amino acids. The theoretical molecular mass of mature Mj-sty was 8.693 kDa with an isoelectric point (pI) of 4.79. A proline-rich N-terminal region and a C-terminal region contained 13 cysteine residues were identified...
December 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26161497/assessment-and-application-of-density-functional-theory-for-the-prediction-of-structure-and-reactivity-of-vanadium-complexes
#3
Brendan J H Sheppard, Michael P Shaver, Jason K Pearson
We assess the performance of six density functionals, each paired with one of five basis sets (a total of 30 model chemistries) for the prediction of geometrical parameters in the coordination sphere of nine vanadium complexes (for a total of 270 structural analyses). We find that results are generally consistent over the range of functionals tested and that none fail drastically. For bond lengths, the model chemistry PBE0/QZVP performed the best overall (having a MAD of only 0.02 Å from experiment) yet PBE0/6-31G* provides nearly identical results...
August 6, 2015: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/21619370/quantitative-analysis-and-structure-determination-of-styrene-methyl-methacrylate-copolymers-by-pyrolysis-gas-chromatography
#4
F C Wang, P B Smith
A pyrolysis gas chromatography (Py-GC) method has been developed to study the composition and microstructure of styrene/methyl methacrylate (STY/MMA) copolymers. The composition was quantified by Py-GC using monomer peak intensity. Because of the poor stability of methyl methacrylate oligomers, neither MMA dimer nor MMA trimers were detected under normal pyrolysis conditions. The number-average sequence length for STY was determined by pure and hybrid trimer peak intensities. The number-average sequence length for MMA was determined by using formulas that incorporate composition and the number-average sequence length of STY...
September 1, 1996: Analytical Chemistry
https://www.readbyqxmd.com/read/19370094/a-y-genome-specific-sts-marker-in-pseudoroegneria-and-elymus-species-triticeae-gramineae
#5
Pungu Okito, Ivan W Mott, Yajun Wu, Richard R-C Wang
The tribe Triticeae Dumortier in the grass family (Poaceae) includes the most important cereal crops (e.g., wheat, barley, and rye) and some economically important forage grasses. Elymus L. is the largest and most complex genus in the Triticeae tribe with approximately 150 species occurring worldwide. The genomic constitutions of approximately 40% of Elymus species are unknown and some have unverified genomic combinations. Of those known for genome constitutions, Elymus species have a genomic formula of StH, StP, StY, StStY, StHY, StPY, or StWY...
April 2009: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/17710618/genotype-based-differentiation-of-the-cryptococcus-neoformans-serotypes-by-combined-pcr-rflp-analysis-of-the-capsule-associated-genes-cap10-and-cap59
#6
Alessandro Raimondi, Rosalia Ticozzi, Giuliana Sala, Maria Grazia Bellotti
This report describes an indirect identification method for Cryptococcus neoformans serotypes developed using combined restriction enzyme pattern analysis of two PCR-amplified portions of the capsule-associated genes CAP10 and CAP59. The method relies on the recognition of the sequence conformation of nine serotype-related polymorphic sites by the analysis of four restriction profiles. A 610 nucleotides long trait of the CAP10 gene was digested with the enzymes Sty I or Sal I and a 597 nucleotides long trait of the CAP59 gene was digested with the enzymes Sal I or EcoRV+PstI...
September 2007: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/17511489/formation-of-tethered-polyacrylic-acid-loops-in-core-shell-micelles
#7
COMPARATIVE STUDY
Michael R Whittaker, Carl N Urbani, Michael J Monteiro
Well-defined amphiphilic four-arm star P(AA-b-STY) block copolymers have been dispersed in water to form core-shell micelles in which the shell consists of tethered PAA loops. The entropic penalty for having such loops resulted in a less densely packed PSTY core when compared to linear diblock copolymers of the same arm length. The surface of the shell is irregular because of the tethering points, but when cleaved the PAA chains extend to form a regular and relatively uniform corona.
July 17, 2007: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/16085828/genetic-characterization-of-accumulation-of-polyhydroxyalkanoate-from-styrene-in-pseudomonas-putida-ca-3
#8
Niall D O'Leary, Kevin E O'Connor, Patrick Ward, Miriam Goff, Alan D W Dobson
Pseudomonas putida CA-3 is capable of accumulating medium-chain-length polyhydroxyalkanoates (MCL-PHAs) when growing on the toxic pollutant styrene as the sole source of carbon and energy. In this study, we report on the molecular characterization of the metabolic pathways involved in this novel bioconversion. With a mini-Tn5 random mutagenesis approach, acetyl-coenzyme A (CoA) was identified as the end product of styrene metabolism in P. putida CA-3. Amplified flanking-region PCR was used to clone functionally expressed phenylacetyl-CoA catabolon genes upstream from the sty operon in P...
August 2005: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/16045812/protein-kinase-clk-sty-is-differentially-regulated-during-erythroleukemia-cell-differentiation-a-bias-toward-the-skipped-splice-variant-characterizes-postcommitment-stages
#9
Ana García-Sacristán, María J Fernández-Nestosa, Pablo Hernández, Jorge B Schvartzman, Dora B Krimer
Clk/STY is a LAMMER protein kinase capable to phosphorylate serine/arginine-rich (SR) proteins that modulate pre-mRNA splicing. Clk/STY alternative splicing generates transcripts encoding a full-length kinase and a truncated catalytically inactive protein. Here we showed that clk/STY, as well as other members of the family (e.g. clk2, clk3 and clk4), are up-regulated during HMBA-induced erythroleukemia cell differentiation. mRNAs coding for the full-length and the truncated forms were responsible for the overall increased expression...
July 2005: Cell Research
https://www.readbyqxmd.com/read/14963652/phylogenetic-and-evolutionary-relationships-between-elymus-humidus-and-other-elymus-species-based-on-sequencing-of-non-coding-regions-of-cpdna-and-aflp-of-nuclear-dna
#10
D H Xu, T Ban
Species of the genus Elymus are closely related to some important cereal crops and may thus serve as potential alien genetic resources for the improvement of these crops. E. humidus is indigenous to Japan and is well adapted to a humid climate. However, the phylogenetic and evolutionary relationships between E. humidus and other Elymus species are unclear. To elucidate these relationships, we examined the sequences of three non-coding regions of chloroplast DNA (cpDNA) and the amplified fragment length polymorphism (AFLP) variation of nuclear DNA in E...
May 2004: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/12713162/molecular-characterization-of-infectious-bursal-disease-virus-from-commercial-poultry-in-the-united-states-and-latin-america
#11
A Banda, P Villegas, J El-Attrache
From June 1999 to September 2001, 216 bursal samples from broiler farms in the United States and from countries of Latin America were submitted to the Poultry Diagnostic and Research Center at the University of Georgia for the purpose of genotyping field infectious bursal disease viruses (IBDVs). The reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify a 248-bp product, encompassing the hypervariable region of VP2 gene. The genotyping was conducted by restriction fragment length polymorphism (RFLP) analysis with six restriction endonucleases, DraI, SacI, TaqI, Sty, BstNI, and SspI...
January 2003: Avian Diseases
https://www.readbyqxmd.com/read/10784412/a-novel-van91-i-polymorphism-in-the-1st-intron-of-the-parathyroid-hormone-pth-pth-related-peptide-pthrp-receptor-gene-and-its-effect-on-the-urinary-camp-response-to-pth
#12
M Heishi, H Tazawa, T Matsuo, T Saruta, M Hanaoka, Y Tsukamoto
This study was designed to identify a parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor gene polymorphism in a healthy Japanese population. All known 13 introns of this gene were amplified by PCR, except the 1st intron, which was amplified by the long-PCR method. No restriction fragment length polymorphisms (RFLPs) were detected by BsmI or XbaI in any of these introns. Twenty-one other restriction enzymes (Hind III, Bgl II, Sty I, Pvu II, Eco81 I, Van91 I, BstX I, Sse8387 I, EcoR I, BamH I, Mbo II, Tth111 I, PshA I, Eam1105 I, Not I, Srf I, Bgl I, Fok I, Sfi I, Apa I, Taq I) were tested on the 1st intron...
April 2000: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/10593939/an-sc35-like-protein-and-a-novel-serine-arginine-rich-protein-interact-with-arabidopsis-u1-70k-protein
#13
M Golovkin, A S Reddy
The U1 small nuclear ribonucleoprotein 70-kDa protein, a U1 small nuclear ribonucleoprotein-specific protein, has been shown to have multiple roles in nuclear precursor mRNA processing in animals. By using the C-terminal arginine-rich region of Arabidopsis U1-70K protein in the yeast two-hybrid system, we have identified an SC35-like (SR33) and a novel plant serine/arginine-rich (SR) protein (SR45) that interact with the plant U1-70K. The SR33 and SR45 proteins share several features with SR proteins including modular domains typical of splicing factors in the SR family of proteins...
December 17, 1999: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/10573334/photopenic-bone-scan-osteomyelitis-a-clinical-perspective
#14
W T Pennington, M P Mott, J G Thometz, J R Sty, D Metz
Eighty-one pediatric patients had nuclear bone-imaging studies confirmatory of osteomyelitis during the past 10 years. Seven (8.6%) of 81 had "cold" osteomyelitis. These seven patients were all toxic [mean temperature (T), 39.9 degrees C; heart rate (HR), 145 beats/min], all had markedly elevated erythrocyte sedimentation rates (mean ESR, 76 mm/h), and six of seven had both confirmatory bone and blood cultures. All patients required surgical intervention. The average length of hospital stay for these seven patients was 32 days (range, 8-65 days)...
November 1999: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/9864041/hla-b-27-subtyping-by-pcr-rflp-in-spanish-patients-with-ankylosing-spondylitis
#15
A Fraile, J Martin, M A López-Nevot, L Mataran, A Nieto
We describe the use of restriction analysis on PCR-amplified DNA for detecting all B*27 subtypes except B*2710 and B*2711 (i.e. from B*2701 to B*2709). After detecting B*27 by Sty I, double digestions consisting of Sty I plus another informative enzyme led to subtype assignment. We used mismatched primers to create restriction sites when necessary. The method avoids group-specific amplifications and other laborious optimization procedures. It was successfully tested on a panel of well characterized cell lines covering different B*27 subtypes...
November 1998: Tissue Antigens
https://www.readbyqxmd.com/read/8798720/srpk1-and-clk-sty-protein-kinases-show-distinct-substrate-specificities-for-serine-arginine-rich-splicing-factors
#16
K Colwill, L L Feng, J M Yeakley, G D Gish, J F Cáceres, T Pawson, X D Fu
Serine/arginine-rich (SR) proteins are essential for pre-mRNA splicing, and modify the choice of splice site during alternative splicing in a process apparently regulated by protein phosphorylation. Two protein kinases have been cloned that can phosphorylate SR proteins in vitro: SRPK1 and Clk/Sty. Here, we show that these two kinases phosphorylate the same SR proteins in vitro, but that SRPK1 has the higher specific activity toward ASF/SF2. SRPK1, like Clk/Sty, phosphorylates ASF/SF2 in vitro on sites that are also phosphorylated in vivo...
October 4, 1996: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/7665564/alternative-splicing-of-sty-a-nuclear-dual-specificity-kinase
#17
P I Duncan, B W Howell, R M Marius, S Drmanic, E M Douville, J C Bell
The LAMMER subfamily of kinases has been conserved throughout evolution, and its members are thought to play important roles in the regulation of cellular growth and differentiation programs. STY is a murine LAMMER kinase which has been implicated in the control of PC12 cell differentiation. Multiple transcripts are derived from the Sty gene, and their relative abundance is developmentally regulated. Alternative splicing of the primary Sty transcript generates mRNAs encoding full-length catalytically active (STY) and truncated, kinase-deficient polypeptides...
September 15, 1995: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/7583566/a-new-case-of-apoa-i-deficiency-showing-codon-8-nonsense-mutation-of-the-apoa-i-gene-without-evidence-of-coronary-heart-disease
#18
K Takata, K Saku, T Ohta, M Takata, H Bai, S Jimi, R Liu, H Sato, G Kajiyama, K Arakawa
We report a 39-year-old Japanese man with HDL and apoA-I deficiency as well as data from members of his family. Corneal opacity and a stomatocyte were found but not tonsillar hypertrophy, xanthomas, or splenomegaly. His serum HDL cholesterol, apoA-I, apoA-II, and LDL cholesterol levels were t mg/dL, < 3 mg/dL, 6 mg/dL, and 175 mg/dL, respectively. Plasma triglyceride, phospholipid, apoB, apoC-III, and apoE levels were all within normal limits. Lecithin:cholesterol acyltransferase activity was half of normal, while lipoprotein lipase and hepatic triglyceride lipase activities were within normal limits...
November 1995: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/2678475/stabilization-of-z-dna-in-vivo-by-localized-supercoiling
#19
A R Rahmouni, R D Wells
Biological processes such as transcription may generate domains of supercoiling on a circular DNA. The existence of these domains in Escherichia coli was investigated by the ability of different lengths of (CG) tracts, cloned upstream or downstream from the tetracycline resistance gene (tet) of pBR322, to adopt the Z structure in vivo. Segments as short as 12 base pairs adopt the Z form when cloned upstream from the tet gene (Eco RI site), whereas no Z DNA was detected when this sequence was cloned downstream (Sty I site), even with a 74-base pair (CG) tract that requires less supercoiling than shorter tracts for the B-Z transition...
October 20, 1989: Science
https://www.readbyqxmd.com/read/2211743/blood-compatibility-of-peo-grafted-polyurethane-and-hema-styrene-block-copolymer-surfaces
#20
COMPARATIVE STUDY
C Nojiri, T Okano, H A Jacobs, K D Park, S F Mohammad, D B Olsen, S W Kim
HEMA/styrene (HEMA/STY) block copolymers and poly(ethylene oxide) 4,000 M.W. (PEO4K) grafted Biomer (B-PEO4K) surfaces have been synthesized, characterized, and evaluated as blood-contacting materials. These surfaces have demonstrated improved blood compatibility, compared to Biomer, in in vitro and ex vivo experiments. Biomer vascular grafts (6 mm I.D. 7 cm in length) were fabricated by a dip coating process. The luminal surface was modified either with PEO grafting, HEMA/STY coating, or Biomer coating (control)...
September 1990: Journal of Biomedical Materials Research
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