Zina M Uckeley, Rebecca Moeller, Lars I Kühn, Emma Nilsson, Claudia Robens, Lisa Lasswitz, Richard Lindqvist, Annasara Lenman, Vania Passos, Yannik Voss, Christian Sommerauer, Martin Kampmann, Christine Goffinet, Felix Meissner, Anna K Överby, Pierre-Yves Lozach, Gisa Gerold
Novel tick-borne phleboviruses in the Phenuiviridae family, which are highly pathogenic in humans and all closely related to Uukuniemi virus (UUKV), have recently emerged on different continents. How phleboviruses assemble, bud, and exit cells remains largely elusive. Here, we performed high-resolution, label-free mass spectrometry analysis of UUKV immunoprecipitated from cell lysates and identified 39 cellular partners interacting with the viral envelope glycoproteins. The importance of these host factors for UUKV infection was validated by silencing each host factor by RNA interference...
December 2019: Molecular & Cellular Proteomics: MCP