keyword
MENU ▼
Read by QxMD icon Read
search

deaminase

keyword
https://www.readbyqxmd.com/read/28812413/unusual-retinopathy-in-a-child-with-severe-combined-immune-deficiency
#1
Christina Gerth-Kahlert, Amit Tiwari, Mathias M Hauri-Hohl, James V M Hanson, Angela Bahr, Anja Palmowski-Wolfe, Tayfun Güngör, Wolfgang Berger
We describe a case of an infant diagnosed with severe combined immune deficiency (Adenosine Deaminase (ADA), SCID) with severe retinopathy and associated low vision in both eyes at first examination. An extensive infectious work up revealed an enterovirus infection, which suggested an early infectious and severe retinopathy. Genetic causes of congenital retinitis pigmentosa/ Leber's congenital amaurosis could be excluded by whole exome sequencing.
August 16, 2017: Ophthalmic Genetics
https://www.readbyqxmd.com/read/28809145/moderate-sensitivity-of-mouse-mammary-tumour-virus-to-inhibition-by-human-apobec3g
#2
Constantine James Konstantoulas, Benedikt Hagen, Stanislav Indik
Infectivity of the mouse mammary tumour virus (MMTV) is inhibited by mouse APOBEC3 (mA3) which is efficiently packaged into virions. As the inhibition is only partial, the virus can replicate in tissues expressing mA3 and complete its replication cycle. Here, we have examined the sensitivity of MMTV to inhibition by a human orthologue of mA3, A3G. We report that the virus containing A3G is only moderately susceptible to inhibition by the human factor. Whereas the vif-deficient HIV-1 vector produced in human epithelial cells expressing endogenous levels of A3G was efficiently inhibited, an MMTV vector remained fully infectious...
August 15, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28808129/gene-duplication-in-pseudomonas-aeruginosa-improves-growth-on-adenosine
#3
Jean-Paul Toussaint, Anna Farrell-Sherman, Tamar Perla Feldman, Nicole E Smalley, Amy L Schaefer, E Peter Greenberg, Ajai A Dandekar
The laboratory strain of Pseudomonas aeruginosa, PAO1, activates genes for catabolism of adenosine using quorum sensing (QS). However, this strain is not well-adapted for growth on adenosine, with doubling times greater than 40 h. We previously showed that when PAO1 is grown on adenosine and casein, variants emerge that grow rapidly on adenosine. To understand the mechanism by which this adaptation occurs, we performed whole-genome sequencing of five isolates evolved for rapid growth on adenosine. All five genomes had a gene duplication-amplification (GDA) event covering several genes, including the quorum-regulated nucleoside hydrolase, nuh, and an adenine deaminase encoded by PA0148...
August 14, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28807888/spectroscopic-and-calorimetric-assays-reveal-dependence-on-dctp-and-two-metals-zn-2-mg-2-for-enzymatic-activity-of-schistosoma-mansoni-deoxycytidylate-dcmp-deaminase
#4
Jéssica Fernandes Scortecci, Vitor Hugo Balasco Serrão, Juliana Cheleski, Juliana Roberta Torini, Larissa Romanello, Ricardo DeMarco, Humberto D'Muniz Pereira
The parasite Schistosoma mansoni possess all pathways for pyrimidine biosynthesis, whereby deaminases play an essential role in the thymidylate cycle, a crucial step to controlling the ratio between cytidine and uridine nucleotides. In this study, we heterologously expressed and purified the deoxycytidylate (dCMP) deaminase from S. mansoni to obtain structural, biochemical and kinetic information. Small-angle X-ray scattering of this enzyme showed that it is organized as a hexamer in solution. Isothermal titration calorimetry was used to determine the kinetic constants for dCMP-dUMP conversion and the role of dCTP and dTTP in enzymatic regulation...
August 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28805790/successful-reduced-intensity-hematopoietic-cell-transplant-in-a-patient-with-deficiency-of-adenosine-deaminase-2
#5
H Hashem, A Vatsayan, A Gupta, K Nagle, M Hershfield, J Dalal
No abstract text is available yet for this article.
August 14, 2017: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/28798173/draft-genome-sequence-of-the-endophytic-bacillus-aryabhattai-strain-squ-r12-identified-from-phoenix-dactylifera-l-roots
#6
Mahmoud W Yaish
Bacillus aryabhattai strain SQU-R12 was isolated from date palm seedlings, where it showed a growth-promoting capacity by being able to synthesize indole-3-acetic acid phytohormone and reduce ethylene biosynthesis by producing 1-aminocyclopropane-1-carboxylic acid deaminase. The draft genome sequence of this strain is reported here.
August 10, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28796151/potential-development-of-tumor-targeted-oral-anti-cancer-prodrugs-amino-acid-and-dipeptide-monoester-prodrugs-of-gemcitabine
#7
Yasuhiro Tsume, Adam J Drelich, David E Smith, Gordon L Amidon
One of the main obstacles for cancer therapies is to deliver medicines effectively to target sites. Since stroma cells are developed around tumors, chemotherapeutic agents have to go through stroma cells in order to reach tumors. As a method to improve drug delivery to the tumor site, a prodrug approach for gemcitabine was adopted. Amino acid and dipeptide monoester prodrugs of gemcitabine were synthesized and their chemical stability in buffers, resistance to thymidine phosphorylase and cytidine deaminase, antiproliferative activity, and uptake/permeability in HFF cells as a surrogate to stroma cells were determined and compared to their parent drug, gemcitabine...
August 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28790991/bacteria-associated-to-plants-naturally-selected-in-a-historical-pcb-polluted-soil-show-potential-to-sustain-natural-attenuation
#8
Lorenzo Vergani, Francesca Mapelli, Ramona Marasco, Elena Crotti, Marco Fusi, Antonio Di Guardo, Stefano Armiraglio, Daniele Daffonchio, Sara Borin
The exploitation of the association between plants and microorganisms is a promising approach able to boost natural attenuation processes for soil clean-up in vast polluted areas characterized by mixed chemical contamination. We aimed to explore the selection of root-associated bacterial communities driven by different plant species spontaneously established in abandoned agricultural soils within a historical polluted site in north Italy. The site is highly contaminated by chlorinated persistent organic pollutants, mainly constituted by polychlorobiphenyls (PCBs), together with heavy metals and metalloids, in variable concentrations and uneven distribution...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28790320/activation-induced-cytidine-deaminase-targets-suv4-20-mediated-histone-h4k20-trimethylation-to-class-switch-recombination-sites
#9
Virginia C Rodríguez-Cortez, Paloma Martínez-Redondo, Francesc Català-Moll, Javier Rodríguez-Ubreva, Antonio Garcia-Gomez, Ganesh Poorani-Subramani, Laura Ciudad, Henar Hernando, Arantxa Pérez-García, Carlos Company, José M Urquiza, Almudena R Ramiro, Javier M Di Noia, Alejandro Vaquero, Esteban Ballestar
Activation-induced cytidine deaminase (AID) triggers antibody diversification in B cells by catalysing deamination and subsequently mutating immunoglobulin (Ig) genes. Association of AID with RNA Pol II and occurrence of epigenetic changes during Ig gene diversification suggest participation of AID in epigenetic regulation. AID is mutated in hyper-IgM type 2 (HIGM2) syndrome. Here, we investigated the potential role of AID in the acquisition of epigenetic changes. We discovered that AID binding to the IgH locus promotes an increase in H4K20me3...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28782586/breaking-the-mirror-l-amino-acid-deaminase-a-novel-stereoselective-biocatalyst
#10
REVIEW
Gianluca Molla, Roberta Melis, Loredano Pollegioni
Enantiomerically pure amino acids are of increasing interest for the fine chemical, agrochemicals and pharmaceutical industries. During past years l-amino acids have been produced from deracemization of dl-solution employing the stereoselective flavoenzyme d-amino acid oxidase. On the other hand, the isolation of corresponding d-isomer was hampered by the scarce availability of a suitable l-amino acid oxidase activity. On this side, l-amino acid deaminase (LAAD), only present in the Proteus bacteria, represents a suitable alternative...
August 3, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28772215/treatment-with-tucum%C3%A3-oil-astrocaryum-vulgare-for-diabetic-mice-prevents-changes-in-seric-enzymes-of-the-purinergic-system-improvement-of-immune-system
#11
Matheus D Baldissera, Carine F Souza, Pedro H Doleski, Thirssa H Grando, Michele R Sagrillo, Aleksandro S da Silva, Daniela B R Leal, Silvia G Monteiro
The aim of this study was to evaluate the enzymatic activity of the purinergic system in sera samples from alloxan-induced diabetic mice treated with tucumã oil (Astrocaryum vulgare). For this, the mice were divided into four groups (n=6): control/water (the group CW); control/tucumã oil (the group CT); diabetic/water (the group DW), and diabetic/tucumã oil (the group DT) treated for 14days with 5.0mLkg(-1) via oral gavage. On day 14 post-treatment, mice were submitted to euthanasia and blood samples were collected by cardiac puncture...
July 31, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28771547/evaluation-of-multifarious-plant-growth-promoting-traits-antagonistic-potential-and-phylogenetic-affiliation-of-rhizobacteria-associated-with-commercial-tea-plants-grown-in-darjeeling-india
#12
Jintu Dutta, Debajit Thakur
Plant growth promoting rhizobacteria (PGPR) are studied in different agricultural crops but the interaction of PGPR of tea crop is not yet studied well. In the present study, the indigenous tea rhizobacteria were isolated from seven tea estates of Darjeeling located in West Bengal, India. A total of 150 rhizobacterial isolates were screened for antagonistic activity against six different fungal pathogens i.e. Nigrospora sphaerica (KJ767520), Pestalotiopsis theae (ITCC 6599), Curvularia eragostidis (ITCC 6429), Glomerella cingulata (MTCC 2033), Rhizoctonia Solani (MTCC 4633) and Fusarium oxysporum (MTCC 284), out of which 48 isolates were antagonist to at least one fungal pathogen used...
2017: PloS One
https://www.readbyqxmd.com/read/28770471/effect-of-decaffeinated-coffee-on-function-and-nucleotide-metabolism-in-kidney
#13
I M Rybakowska, R Milczarek, E M Slominska, R T Smolenski
Little is known about the effects of coffee that are not related to the presence of caffeine. The aim of the study was to analyse changes in kidney function and nucleotide metabolism related to high intake of decaffeinated coffee. Mice consumed decaffeinated coffee extract for two weeks. Activities of AMP deaminase, ecto5'-nucleotidase, adenosine deaminase, purine nucleoside phosphorylase were measured in kidney cortex and medulla by analysis of conversion of substrates into products using HPLC. Concentration of nucleotides in kidney cortex, kidney medulla and serum were estimated by HPLC...
August 2, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28769881/genetic-diversity-of-nitrogen-fixing-and-plant-growth-promoting-pseudomonas-species-isolated-from-sugarcane-rhizosphere
#14
Hai-Bi Li, Rajesh K Singh, Pratiksha Singh, Qi-Qi Song, Yong-Xiu Xing, Li-Tao Yang, Yang-Rui Li
The study was designed to isolate and characterize Pseudomonas spp. from sugarcane rhizosphere, and to evaluate their plant- growth- promoting (PGP) traits and nitrogenase activity. A biological nitrogen-fixing microbe has great potential to replace chemical fertilizers and be used as a targeted biofertilizer in a plant. A total of 100 isolates from sugarcane rhizosphere, belonging to different species, were isolated; from these, 30 isolates were selected on the basis of preliminary screening, for in vitro antagonistic activities against sugarcane pathogens and for various PGP traits, as well as nitrogenase activity...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28765186/tuberculous-pericarditis-treated-with-steroid-in-a-dialysis-patient
#15
Rie Aoyama, Joji Ishikawa, Kazumasa Harada, Yayoi Tukada
Mycobacterium tuberculosis has spread worldwide and its mortality rate had been very high. The prevention technology and antituberculosis (TB) chemotherapy has improved its prognosis. However, immunocompromised patients, such as those who had HIV infection, older age and on haemodialysis, are still at high risk of TB infection. TB pericarditis is a common cause of constrictive pericarditis and its mortality remains high. Early diagnosis and initiation of appropriate therapy are critical to improve mortality...
August 1, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28762759/adenosine-to-inosine-rna-editing-in-health-and-disease
#16
Aikaterini Gatsiou, Nikolaos Vlachogiannis, Federica Francesca Lunella, Marco Sachse, Konstantinos Stellos
SIGNIFICANCE: Adenosine deaminase in transcriptome results in formation of inosine, a process that is called A-to-I RNA editing. Adenosine deaminase is one of the more than 140 described RNA modifications. A-to-I RNA editing is catalysed by the adenosine deaminases acting on RNA (ADAR) enzymes and is essential for life. Recent Advances: Accumulating evidence supports a critical role of RNA editing in all aspects of RNA metabolism including mRNA stability, splicing, nuclear export and localisation, as well as in recoding of proteins...
August 1, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28761003/multiple-ppr-protein-interactions-are-involved-in-the-rna-editing-system-in-arabidopsis-mitochondria-and-plastids
#17
Nuria Andrés-Colás, Qiang Zhu, Mizuki Takenaka, Bert De Rybel, Dolf Weijers, Dominique Van Der Straeten
Recent identification of several different types of RNA editing factors in plant organelles suggests complex RNA editosomes within which each factor has a different task. However, the precise protein interactions between the different editing factors are still poorly understood. In this paper, we show that the E(+)-type pentatricopeptide repeat (PPR) protein SLO2, which lacks a C-terminal cytidine deaminase-like DYW domain, interacts in vivo with the DYW-type PPR protein DYW2 and the P-type PPR protein NUWA in mitochondria, and that the latter enhances the interaction of the former ones...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28759788/isolation-of-vanadium-resistance-endophytic-bacterium-pre01-from-pteris-vittata-in-stone-coal-smelting-district-and-characterization-for-potential-use-in-phytoremediation
#18
Liang Wang, Hai Lin, Yingbo Dong, Yinhai He, Chenjing Liu
This study investigates the V-resistant endophytic bacteria isolated from V-accumulator Pteris vittata grown on stone coal smelting district. Among all the ten isolates, the strain PRE01 identified as Serratia marcescens ss marcescens by Biolog GEN III MicroPlate™ was screened out by ranking first in terms of heavy metal resistance and plant growth promoting traits. The S. marcescens PRE01 had strong V, Cr and Cd resistance especially for V up to 1500mg/L. In addition, it exhibited ACC deaminase activity, siderophore production and high indoleacetic acid production (60...
July 24, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28759736/phylloplane-bacteria-of-jatropha-curcas-diversity-metabolic-characteristics-and-growth-promoting-attributes-towards-vigor-of-maize-seedling
#19
Garima Dubey, Bharati Kollah, Usha Ahirwar, Asit Mandal, Jyoti Kumar Thakur, Ashok Kumar Patra, Santosh Ranjan Mohanty
The complex role of phylloplane microorganisms is less understood than the rhizospheric ones in lieu of their pivotal role in plant's sustainability. This experiment aims to study diversity of culturable phylloplane bacteria of Jatropha curcas and evaluate their growth promoting activities towards vigour of maize seedling. Heterotrophic bacteria were isolated from the phylloplane of J. curcas and their 16S rRNA genes were sequenced. Sequence of 16S rRNA gene were closely similar to species belonging to the class Bacillales (50%) Gammaproteobacteria (21...
July 31, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28757211/aid-recognizes-structured-dna-for-class-switch-recombination
#20
Qi Qiao, Li Wang, Fei-Long Meng, Joyce K Hwang, Frederick W Alt, Hao Wu
Activation-induced cytidine deaminase (AID) initiates both class switch recombination (CSR) and somatic hypermutation (SHM) in antibody diversification. Mechanisms of AID targeting and catalysis remain elusive despite its critical immunological roles and off-target effects in tumorigenesis. Here, we produced active human AID and revealed its preferred recognition and deamination of structured substrates. G-quadruplex (G4)-containing substrates mimicking the mammalian immunoglobulin switch regions are particularly good AID substrates in vitro...
August 3, 2017: Molecular Cell
keyword
keyword
53442
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"