keyword
MENU ▼
Read by QxMD icon Read
search

Fusarium

keyword
https://www.readbyqxmd.com/read/28324245/comparison-of-dna-microarray-loop-mediated-isothermal-amplification-lamp-and-real-time-pcr-with-dna-sequencing-for-identification-of-fusarium-spp-obtained-from-patients-with-hematologic-malignancies
#1
Marcela de Souza, Tetsuhiro Matsuzawa, Kanae Sakai, Yasunori Muraosa, Luzia Lyra, Ariane Fidelis Busso-Lopes, Anna Sara Shafferman Levin, Angélica Zaninelli Schreiber, Yuzuru Mikami, Tohoru Gonoi, Katsuhiko Kamei, Maria Luiza Moretti, Plínio Trabasso
The performance of three molecular biology techniques, i.e., DNA microarray, loop-mediated isothermal amplification (LAMP), and real-time PCR were compared with DNA sequencing for properly identification of 20 isolates of Fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. DNA microarray, LAMP and real-time PCR identified 16 (80%) out of 20 samples as Fusarium solani species complex (FSSC) and four (20%) as Fusarium spp. The agreement among the techniques was 100%...
March 21, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28323535/effector-gene-suites-in-some-soil-isolates-of-fusarium-oxysporum-are-not-sufficient-predictors-of-vascular-wilt-in-tomato
#2
Nicolas Jelinski, Karen Broz, Wilfried Jonkers, Li-Jun Ma, Harold Corby Kistler
Seventy-four F. oxysporum soil isolates were assayed for known effector genes present in an F. oxysporum f.sp. lycopersici race 3 tomato wilt strain (FOL MN-25) obtained from the same fields in Manatee County, Florida. Based on the presence or absence of these genes, four haplotypes were defined, two of which represented 96% of the surveyed isolates. These two most common effector haplotypes contained either all or none of the assayed race 3 effector genes. We hypothesized that soil isolates with all surveyed effector genes, similar to FOL MN-25, would be pathogenic toward tomato, whereas isolates lacking all effectors would be non-pathogenic...
March 21, 2017: Phytopathology
https://www.readbyqxmd.com/read/28319793/early-response-of-wheat-antioxidant-system-with-special-reference-to-fusarium-head-blight-stress
#3
Valentina Spanic, Marija Viljevac Vuletic, Ivan Abicic, Tihana Marcek
Fusarium head blight (FHB) is a destructive fungal disease of wheat (Triticum aestivum L.) that causes significant grain yield losses and end-use quality reduction associated with contamination by the mycotoxin deoxynivalenol (DON). Three winter wheat varieties ('Vulkan', 'Kraljica' and 'Golubica') were screened for FHB resistance using artificial inoculation technique under field conditions. The aim of this study was to examine a relationship between FHB resistance and the effectiveness of enzyme antioxidant system of wheat varieties under different sampling times (3, 15, 24, 48, 96, 120 and 336 hai)...
March 14, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28318747/ultra-fast-quantitation-of-voriconazole-in-human-plasma-by-coated-blade-spray-mass-spectrometry
#4
Marcos Tascon, Germán Augusto Gómez-Ríos, Nathaly Reyes-Garcés, Justen Poole, Ezel Boyacı, Janusz Pawliszyn
Voriconazole is a triazole broad-spectrum antifungal medication often used to treat fungal infections caused by Aspergillus and Fusarium species. One of the main challenges associated with the implementation of this medication is its narrow therapeutic concentration range, demonstrating toxicity at concentrations above 6μg/mL and limited efficacy at concentrations below 2μg/mL. As a result, methodologies which permit the rapid and accurate quantitation of voriconazole in patients are highly desirable. In this work two different approaches based on coated blade spray directly coupled to mass spectrometry (CBS-MS) are introduced; each enabling the quantitation of voriconazole in plasma samples with a simple and fast sample preparation and no chromatographic step...
March 12, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28306726/fitness-of-three-chemotypes-of-fusarium-graminearum-species-complex-in-major-winter-wheat-producing-areas-of-china
#5
Yang-Yang Liu, Han-Yan Sun, Wei Li, Yun-Lei Xia, Yuan-Yu Deng, Ai-Xiang Zhang, Huai-Gu Chen
In China, Fusarium head blight is caused mainly by the Fusarium graminearum species complex (FGSC), which produces trichothecene toxins. The FGSC is divided into three chemotypes: 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), and nivalenol (NIV). In order to predict the geographical changes in the distribution of these chemotype populations in major winter wheat-producing areas in China, the biological characteristics of twenty randomly selected isolates from each of the three chemotypes were studied...
2017: PloS One
https://www.readbyqxmd.com/read/28303817/screening-of-invasive-fungal-infections-by-a-real-time-panfungal-pan-acf-polymerase-chain-reaction-assay-in-patients-with-haematological-malignancy
#6
Malini Rajinder Capoor, Shikha Puri, Hitesh Raheja, Ritin Mohindra, Dinesh Kumar Gupta, Pradeep Kumar Verma, Ranadeep Chowdhary
BACKGROUND: Invasive fungal infection (IFI) is a fatal infection in haematology patients. There is an urgent need for reliable screening methods facilitating timely diagnosis and treatment. A real-time panfungal polymerase chain reaction (PCR) assay based on TaqMan technology targeting 18S ribosomal RNA gene was used to screen whole blood specimen obtained from series of Haematology malignancy patients for IFIs. MATERIALS AND METHODS: The panfungal (Pan-ACF) assay was employed to investigate specimen from 133 patients in duplicate with suspected IFI...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303400/two-new-species-of-the-fusarium-fujikuroi-species-complex-isolated-from-the-natural-environment
#7
Tarek A A Moussa, Hassan S Al-Zahrani, Naif M S Kadasa, Sarah A Ahmed, G Sybren de Hoog, Abdullah M S Al-Hatmi
Two new species in the Fusarium fujikuroi species complex (FFSC) are introduced. One of these, represented by strain CBS 454.97 was isolated from plant debris (Striga hermonthica) in the Sudan, while the second, represented by strains CBS 119850 and CBS 483.94, which originated from soil in Australia. Molecular analyses were performed including TEF1 spanning 576 bp region, 860 bp region of rPB2, and 500 bp BT2 region. Phylogenetic trees based on these regions showed that the two species are clearly distinct from all known taxa in the F...
March 16, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28303127/functional-characterization-of-endophytic-fungal-community-associated-with-oryza-sativa-l-and-zea-mays-l
#8
Momota Potshangbam, S Indira Devi, Dinabandhu Sahoo, Gary A Strobel
In a natural ecosystem, the plant is in a symbiotic relationship with beneficial endophytes contributing huge impact on its host plant. Therefore, exploring beneficial endophytes and understanding its interaction is a prospective area of research. The present work aims to characterize the fungal endophytic communities associated with healthy maize and rice plants and to study the deterministic factors influencing plant growth and biocontrol properties against phytopathogens, viz, Pythium ultimum, Sclerotium oryzae, Rhizoctonia solani, and Pyricularia oryzae...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28302768/draft-genome-sequence-of-rhizobium-sp-strain-tbd182-an-antagonist-of-the-plant-pathogenic-fungus-fusarium-oxysporum-isolated-from-a-novel-hydroponics-system-using-organic-fertilizer
#9
Yuichiro Iida, Kazuki Fujiwara, Nobutaka Someya, Makoto Shinohara
Rhizobium sp. strain TBD182, isolated from a novel hydroponics system, is an antagonistic bacterium that inhibits the mycelial growth of Fusarium oxysporum but does not eliminate the pathogen. We report the draft genome sequence of TBD182, which may contribute to elucidation of the molecular mechanisms of its fungistatic activity.
March 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28300780/simultaneous-determination-of-multi-mycotoxins-in-cereal-grains-collected-from-south-korea-by-lc-ms-ms
#10
Dong-Ho Kim, Sung-Yong Hong, Jea Woo Kang, Sung Min Cho, Kyu Ri Lee, Tae Kyung An, Chan Lee, Soo Hyun Chung
An improved analytical method compared with conventional ones was developed for simultaneous determination of 13 mycotoxins (deoxynivalenol, nivalenol, 3-acetylnivalenol, aflatoxin B₁, aflatoxin B₂, aflatoxin G₁, aflatoxin G₂, fumonisin B₁, fumonisin B₂, T-2, HT-2, zearalenone, and ochratoxin A) in cereal grains by liquid chromatography-tandem mass spectrometry (LC/MS/MS) after a single immunoaffinity column clean-up. The method showed a good linearity, sensitivity, specificity, and accuracy in mycotoxin determination by LC/MS/MS...
March 16, 2017: Toxins
https://www.readbyqxmd.com/read/28299481/the-complete-genome-sequence-of-a-double-stranded-rna-mycovirus-from-fusarium-graminearum-strain-hn1
#11
Luan Wang, Shuangchao Wang, Xiufen Yang, Hongmei Zeng, Dewen Qiu, Lihua Guo
The complete nucleotide sequence of a double-stranded RNA (dsRNA) mycovirus, Fusarium graminearum dsRNA virus 5 (FgV5), was identified and characterized. The FgV5 genome comprises two dsRNA genome segments of 2030 bp and 1740 bp. FgV5 dsRNA1 contains a single open reading frame (ORF1), which is predicted to encode a protein of 613 amino acids (aa) with a molecular mass of 70.4 kDa and has a conserved RNA-dependent RNA polymerase (RdRp) motif. FgV5 dsRNA2 is predicted to contain two discontinuous ORFs (ORF2 and ORF3) that code for products of unknown function...
March 15, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28298316/paediatric-infectious-keratitis-a-case-series-of-107-children-presenting-to-a-tertiary-referral-centre
#12
Julia Dutra Rossetto, Kara M Cavuoto, Carla J Osigian, Ta Chen Peter Chang, Darlene Miller, Hilda Capo, Oriel Spierer
BACKGROUND/AIMS: Corneal ulcers can result in severe visual impairment in children. The recent trends of paediatric microbial ulcerative keratitis in the USA are unknown. The purpose of this study is to report the risk factors, microbiological profile and treatment outcomes of paediatric microbial keratitis in South Florida. METHODS: A university-based tertiary eye care centre retrospective case series between 1992 and 2015. Medical records of 107 paediatric patients (age <18 years) with the diagnosis of microbial ulcerative keratitis were analysed...
March 15, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28298196/microbial-detoxification-of-eleven-food-and-feed-contaminating-trichothecene-mycotoxins
#13
Rafiq Ahad, Ting Zhou, Dion Lepp, K Peter Pauls
BACKGROUND: Contamination of agricultural commodities with multiple trichothecene mycotoxins, produced by toxigenic Fusarium species, is a food safety issue, which greatly affects grain production and marketing worldwide. Importantly, exposure to multiple trichothecenes may increase toxicity in animals due to their synergistic and/or additive effects. To address the problem this study aimed to achieve a novel biological trait capable of detoxifying various food and feed contaminating trichothecenes under aerobic and anaerobic conditions and wide range of temperatures...
March 15, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28296081/functional-characterization-of-cytochrome-p450-monooxygenases-in-the-cereal-head-blight-fungus-fusarium-graminearum
#14
Ji Young Shin, Duc-Cuong Bui, Yoonji Lee, Hyejin Nam, Soyun Jung, Miao Fang, Jin-Cheol Kim, Theresa Lee, Hun Kim, Gyung Ja Choi, Hokyoung Son, Yin-Won Lee
Fusarium graminearum is a prominent plant pathogenic fungus causing Fusarium head blight in major cereal crops worldwide. To understand the molecular mechanisms underlying fungal development and virulence, large collections of F. graminearum mutants have been constructed. Cytochrome P450 monooxygenases (P450s) are widely distributed in organisms and are involved in a diverse array of molecular/metabolic processes; however, no systematic functional analysis of P450s has been attempted in filamentous fungi. In this study, we constructed a genome-wide deletion mutant set covering 102 P450s and analyzed these mutants for changes in 38 phenotypic categories, including fungal development, stress responses, and responses to several xenobiotics, to build a comprehensive phenotypic dataset...
March 14, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28295904/a-fungal-n-dimethylallyltryptophan-metabolite-from-fusarium-fujikuroi
#15
Birgit Arndt, Slavica Janevska, Robin Schmid, Florian Hübner, Bettina Tudzynski, Hans-Ulrich Humpf
The range of secondary metabolites (SMs) produced by the rice pathogen Fusarium fujikuroi is quite broad. Several polyketides, nonribosomal peptides and terpenes have been identified. However, no products of dimethylallyltryptophan synthases (DMATSs) have been elucidated so far, though two putative DMATS genes are present in the F. fujikuroi genome. In this study, the product derived from one of the two DMATSs, DMATS1 (FFUJ_09179), has been identified in vivo with the help of the software MZmine 2. Detailed structure elucidation showed that this metabolite is a reversely N-prenylated tryptophan, depicting a rather rare form of prenylation...
March 10, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28295054/microfluidic-device-enabled-quantitative-time-lapse-microscopic-photography-for-phenotyping-vegetative-and-reproductive-phases-in-fusarium-virguliforme-which-is-pathogenic-to-soybean
#16
Jill Marshall, Xuan Qiao, Jordan Baumbach, Jingyu Xie, Liang Dong, Madan K Bhattacharyya
Time-lapse microscopic-photography allows in-depth phenotyping of microorganisms. Here we report development of such a system using a microfluidic device, generated from polydimethylsiloxane and glass slide, placed on a motorized stage of a microscope for conducting time-lapse microphotography of multiple observations in 20 channels simultaneously. We have demonstrated the utility of the device in studying growth, germination and sporulation in Fusarium virguliforme that causes sudden death syndrome in soybean...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28292351/effects-of-selenium-on-fusarium-growth-and-associated-fermentation-products-and-the-relationship-with-chondrocyte-viability
#17
Hong Yin, Yan Zhang, Feng Zhang, Jin Tao Hu, Yu Meng Zhao, Bo Lun Cheng
This study determined the effects of selenium on the growth of Fusarium strains and the effects of products extracted from the fungal cultures on relevant indicators of chondrocytes injury. The results showed that selenium supplementation resulted in differential effects on the mycelial growth of the strains. Levels of the chondrocyte injury indicators, including cell viability, proteoglycan and type II collagen contents and their mRNA expressions, were all reduced to varying degrees when the chondrocytes were incubated with fermentation extracts, the inhibitory effect varied depending on selenium content supplemented to fungal culture media...
February 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28292258/production-of-bp178-a-derivative-of-the-synthetic-antibacterial-peptide-bp100-in-the-rice-seed-endosperm
#18
Laura Montesinos, Mireia Bundó, Esther Badosa, Blanca San Segundo, María Coca, Emilio Montesinos
BACKGROUND: BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories. RESULTS: A synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter...
March 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28291386/occurrence-of-toxigenic-fungi-and-aflatoxin-potential-of-aspergillus-spp-strains-associated-with-subsistence-farmed-crops-in-haiti
#19
Junior Aristil, Giovanni Venturini, Alberto Spada
Subsistence farming and poor storage facilities favor toxigenic fungal contamination and mycotoxin accumulation in staple foods from tropical countries such as Haiti. The present preliminary study was designed to evaluate the occurrence of toxigenic fungi in Haitian foodstuffs to define the mycotoxin risk associated with Haitian crops. The objectives of this research were to determine the distribution of toxigenic fungi in the Haitian crops maize, moringa, and peanut seeds and to screen Aspergillus section Flavi (ASF) isolates for production of aflatoxins B1 and G1 in vitro...
March 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28290512/chemometric-analysis-reveals-links-in-the-formation-of-fragrant-bio-molecules-during-agarwood-aquilaria-malaccensis-and-fungal-interactions
#20
Supriyo Sen, Madhusmita Dehingia, Narayan Chandra Talukdar, Mojibur Khan
Fragrant agarwood, arguably the costliest wood in the world, is formed by plant-fungal interactions in Aquilaria spp. However, very little is known about this fragrant outcome of interaction. Therefore, mimicking the ancient traditions of agarwood production in Assam (Northeast India), a chemometric assessment of the agarwood-fungus interaction was made by chemical profiling (GC-MS) coupled with statistical analysis (principal component, correlation network analysis) across three platforms, viz. callus, juvenile plants and resinous wood-chips with an associated Fusarium...
March 14, 2017: Scientific Reports
keyword
keyword
53441
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"