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https://www.readbyqxmd.com/read/28654215/the-fgsrp1-sr-protein-gene-is-important-for-plant-infection-and-pre-mrna-processing-in-fusarium-graminearum
#1
Yimei Zhang, Xuli Gao, Manli Sun, Huiquan Liu, Jin-Rong Xu
The versatile functions of SR (serine/arginine-rich) proteins in pre-mRNA splicing and processing are modulated by reversible phosphorylation. Previous studies showed that FgPrp4, the only protein kinase among spliceosome components, is important for intron splicing and the FgSrp1 SR protein is phosphorylated at five conserved sites in Fusarium graminearum. In this study, we showed that the Fgsrp1 deletion mutant rarely produced conidia and caused only limited symptoms on wheat heads and corn silks. Deletion of FgSRP1 also reduced ascospore ejection and deoxynivalenol (DON) production...
June 27, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28654073/rapid-deletion-production-in-fungi-via-agrobacterium-mediated-transformation-of-oscar-deletion-constructs
#2
Scott E Gold, Zahi Paz, María D García-Pedrajas, Anthony E Glenn
Precise deletion of gene(s) of interest, while leaving the rest of the genome unchanged, provides the ideal product to determine that particular gene's function in the living organism. In this protocol the OSCAR method of precise and rapid deletion plasmid construction is described. OSCAR relies on the cloning system in which a single recombinase reaction is carried out containing the purified PCR-amplified 5' and 3' flanks of the gene of interest and two plasmids, pA-Hyg OSCAR (the marker vector) and pOSCAR (the assembly vector)...
June 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28652587/novel-loop-mediated-isothermal-amplification-lamp-assay-with-a-universal-qprobe-can-detect-snps-determining-races-in-plant-pathogenic-fungi
#3
Yu Ayukawa, Saeri Hanyuda, Naoko Fujita, Ken Komatsu, Tsutomu Arie
Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol...
June 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28652549/an-evaluation-method-for-suppression-of-pathogenic-fusarium-oxysporum-by-soil-microorganisms-using-the-dilution-plate-technique
#4
Masahiro Mitsuboshi, Yuuzou Kioka, Katsunori Noguchi, Susumu Asakawa
Volume 31, No. 3, Page 307-313, 2016Page 310, Legend for Fig. 4 IncorrectFig. 4. Growth degree of Fusarium oxysporum f. sp. spinaciae for organic fertilizers at each dilution based on an estimation of the ellipse area (A) and extension length (B) of the colony. Cont, compound inorganic fertilizer; SBM, steamed bone meal; CDC, cow dung compost; MI, microbial inoculant. Values show mean of medians of degrees with SE (n=3). The ellipse area and extension length for control plates were 4,475 mm(2) and 36.5 mm, respectively...
2017: Microbes and Environments
https://www.readbyqxmd.com/read/28651356/heterologous-expression-in-pichia-pastoris-and-biochemical-characterization-of-the-unmodified-sulfatase-from-fusarium-proliferatum-le1
#5
Svetlana A Korban, Kirill S Bobrov, Maria A Maynskova, Stanislav N Naryzhny, Olga L Vlasova, Elena V Eneyskaya, Anna A Kulmisnkaya
Sulfatases are a family of enzymes (sulfuric ester hydrolases, EC 3.1.6.-) that catalyze the hydrolysis of a wide array of sulfate esters. To date, despite the discovery of many sulfatase genes and the accumulation of data on numerous sulfated molecules, the number of characterized enzymes that are key players in sulfur metabolism remains extremely limited. While mammalian sulfatases are well studied due to their involvement in a wide range of normal and pathological biological processes, lower eukaryotic sulfatases, especially fungal sulfatases, have not been thoroughly investigated at the biochemical and structural level...
June 23, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28648292/fusarium-proliferatum-causal-agent-of-garlic-bulb-rot-in-spain-genetic-variability-and-mycotoxin-production
#6
Laura Gálvez, Monika Urbaniak, Agnieszka Waśkiewicz, Łukasz Stępień, Daniel Palmero
Fusarium proliferatum is a world-wide occurring fungal pathogen affecting several crops included garlic bulbs. In Spain, this is the most frequent pathogenic fungus associated with garlic rot during storage. Moreover, F. proliferatum is an important mycotoxigenic species, producing a broad range of toxins, which may pose a risk for food safety. The aim of this study is to assess the intraspecific variability of the garlic pathogen in Spain implied by analyses of translation elongation factor (tef-1α) and FUM1 gene sequences as well as the differences in growth rates...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28647758/a-meta-analysis-of-quantitative-trait-loci-for-abiotic-and-biotic-stress-resistance-in-tetraploid-cotton
#7
Abdelraheem Abdelraheem, Feng Liu, Mingzhou Song, Jinfa F Zhang
The number and location of mapped quantitative trait loci (QTL) depend on genetic populations and testing environments. The identification of consistent QTL across genetic backgrounds and environments is a pre-requisite to marker-assisted selection. This study analyzed a total of 661 abiotic and biotic stress resistance QTL based on our previous work and other publications using the meta-analysis software Biomercator. It identified chromosomal regions containing QTL clusters for different resistance traits and hotspots for a particular resistance trait in cotton from 98 QTL for drought tolerance under greenhouse (DT) and 150 QTL in field conditions (FDT), 80 QTL for salt tolerance in the greenhouse conditions (ST), 201 QTL for resistance to Verticillium wilt (VW, Verticillium dahliae), 47 QTL for resistance to Fusarium wilt (FW, Fusarium oxysporum f...
June 24, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28647118/screening-of-wheat-endophytes-as-biological-control-agents-against-fusarium-head-blight-using-two-different-in-vitro-tests
#8
Morgane Comby, Marie Gacoin, Mathilde Robineau, Fanja Rabenoelina, Sébastien Ptas, Joëlle Dupont, Camille Profizi, Fabienne Baillieul
In order to find biological control agents (BCAs) for the management of Fusarium head blight (FHB), a major disease on wheat crops worldwide, 86 microorganisms isolated from inner tissues of wheat plants were discriminated for their ability to inhibit the growth of Fusarium graminearum and Fusarium culmorum by in vitro dual culture assays. A group of 22 strains appeared very effective to inhibit F. graminearum (inhibition of 30-51%) and they were also globally effective in controlling F. culmorum (inhibition of 15-53%)...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647117/isolation-of-a-potential-biocontrol-agent-paenibacillus-polymyxa-nsy50-from-vinegar-waste-compost-and-its-induction-of-host-defense-responses-against-fusarium-wilt-of-cucumber
#9
Nanshan Du, Lu Shi, Yinghui Yuan, Jin Sun, Sheng Shu, Shirong Guo
Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC) is one of the major destructive soil-borne diseases infecting cucumber. In this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. The 16S rDNA gene demonstrated that the biocontrol agents were Paenibacillus polymyxa (P. polymyxa), Bacillus amyloliquefaciens (B. amyloliquefaciens) and Bacillus licheniformis (B...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28645650/isolation-identification-and-biocontrol-of-antagonistic-bacterium-against-botrytis-cinerea-after-tomato-harvest
#10
Jun-Feng Shi, Chang-Qing Sun
Tomato is one of the most important vegetables in the world. Decay after harvest is a major issue in the development of tomato industry. Currently, the most effective method for controlling decay after harvest is storage of tomato at low temperature combined with usage of chemical bactericide; however, long-term usage of chemical bactericide not only causes pathogen resistance but also is harmful for human health and environment. Biocontrol method for the management of disease after tomato harvest has great practical significance...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28644879/identification-of-a-novel-fungus-trichoderma-asperellum-gdfs1009-and-comprehensive-evaluation-of-its-biocontrol-efficacy
#11
Qiong Wu, Ruiyan Sun, Mi Ni, Jia Yu, Yaqian Li, Chuanjin Yu, Kai Dou, Jianhong Ren, Jie Chen
Due to its efficient broad-spectrum antimicrobial activity, Trichoderma has been established as an internationally recognized biocontrol fungus. In this study, we found and identified a novel strain of Trichoderma asperellum, named GDFS1009. The mycelium of T. asperellum GDFS1009 exhibits a high growth rate, high sporulation capacity, and strong inhibitory effects against pathogens that cause cucumber fusarium wilt and corn stalk rot. T. asperellum GDFS1009 secretes chitinase, glucanase, and protease, which can degrade the cell walls of fungi and contribute to mycoparasitism...
2017: PloS One
https://www.readbyqxmd.com/read/28643581/a-white-paper-on-global-wheat-health-based-on-scenario-development-and-analysis
#12
Serge Savary, Annika Djurle, Jonathan Yuen, Andrea Ficke, Vittorio Rossi, Paul Esker, Jose M Fernandes, Emerson Del Ponte, Jatindra Kumar, Laurence V Madden, Pierce A Paul, Neil McRoberts, Pawan Kumar Singh, Laurent Huber, Claude Pope de Vallavieille, Sébastien Saint-Jean, Laetitia Willocquet
Scenario analysis constitutes a useful approach to synthesize knowledge and derive hypotheses in the case of complex systems which are documented with mainly qualitative or very diverse information. In this article, a framework for scenario analysis is designed and then, applied to global wheat health within a timeframe from today to 2050. Scenario analysis entails the choice of settings, the definition of scenarios of change, and the analysis of outcomes of these scenarios in the chosen settings. Three idealized agrosystems, representing a large fraction of the global diversity of wheat-based agrosystems, are considered, which represent the settings of the analysis...
June 23, 2017: Phytopathology
https://www.readbyqxmd.com/read/28643182/fvstr1-a-striatin-orthologue-in-fusarium-virguliforme-is-required-for-asexual-development-and-virulence
#13
Kazi T Islam, Jason P Bond, Ahmad M Fakhoury
The soil-borne fungus Fusarium virguliforme causes sudden death syndrome (SDS), one of the most devastating diseases of soybean in North and South America. Despite the importance of SDS, a clear understanding of the fungal pathogenicity factors that affect the development of this disease is still lacking. We have identified FvSTR1, a F. virguliforme gene, which encodes a protein similar to a family of striatin proteins previously reported to regulate signalling pathways, cell differentiation, conidiation, sexual development, and virulence in filamentous fungi...
June 22, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28641471/evaluation-of-immunomodulatory-effects-of-zearalenone-in-mice
#14
Mohammad Rafiqul Islam, Jong Won Kim, Yoon-Seok Roh, Jong-Hoon Kim, Han Kang Min, Hyung-Joo Kwon, Chae Woong Lim, Bumseok Kim
Zearalenone (ZEA) is a non-steroidal estrogenic mycotoxin produced by Fusarium species. The toxicity of ZEA has been evaluated for reproductive and developmental effects; however, there is little evidence about its acute toxicity or general immunotoxicity. In the present study, immune regulatory functions were investigated in mice that had been exposed to ZEA (5 or 20 mg/kg BW) daily for 14 days. Results showed that sub-populations of CD4(+), CD8(+) and CD11c(+) cells in the spleen and CD4(+), CD8(+) and F4/80(+) cells in the mesenteric lymph nodes (MLN) of ZEA (20 mg/kg)-exposed hosts were decreased compared to those in the control mice...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28640741/an-endophytic-fusarium-sp-isolated-from-monarda-citriodora-produces-the-industrially-important-plant-like-volatile-organic-compound-hexanal
#15
Meenu Katoch, Kushal Bindu, Shipra Phull, M K Verma
An endophytic fungus, MC_25L, has been isolated from the leaves of MonardacitriodoraCerv. ex Lag., a medicinal and aromatic herb from the northwestern Himalayas. It produces a fruity fragrance while growing on potato dextrose agar, suggesting that it is producing volatile organic compounds (VOCs). The endophyte inhibited the growth of plant pathogens such asSclerotiniasp. and Aspergillusflavus by virtue of VOCs. Identification of MC_25L based on morphological and microscopic features, as well as ITS-based rDNA sequence analysis, revealed that it is a Fusariumsp...
June 22, 2017: Microbiology
https://www.readbyqxmd.com/read/28640201/deoxynivalenol-biomarkers-in-the-urine-of-uk-vegetarians
#16
Liz Wells, Laura Hardie, Courtney Williams, Kay White, Yunru Liu, Barbara De Santis, Francesca Debegnach, Georgio Moretti, Stephanie Greetham, Carlo Brera, Maria Papageorgiou, Natalie J Thatcher, Alan Rigby, Stephen L Atkin, Thozhukat Sathyapalan
Deoxynivalenol (DON) is produced by Fusarium graminearum and is one of the most commonly occurring trichothecenes. Vegetarians are alleged to be a high-risk group for DON exposure due to high intakes of cereals susceptible to the growth of the mycotoxin. This study provides the levels of DON and de-epoxi Deoxynivalenol (DOM-1) in urine analysed by liquid chromatography-mass spectrometry (LC-MS) in UK vegetarians. Over two consecutive days, morning urine samples were collected from 32 vegetarians and 31 UK adult volunteers, and associated food consumption 24 h prior to the sample was recorded...
June 22, 2017: Toxins
https://www.readbyqxmd.com/read/28640190/trans-cinnamic-and-chlorogenic-acids-affect-the-secondary-metabolic-profiles-and-ergosterol-biosynthesis-by-fusarium-culmorum-and-f-graminearum-sensu-stricto
#17
Tomasz Kulik, Kinga Stuper-Szablewska, Katarzyna Bilska, Maciej Buśko, Anna Ostrowska-Kołodziejczak, Dariusz Załuski, Juliusz Perkowski
Plant-derived compounds limiting mycotoxin contamination are currently of major interest in food and feed production. However, their potential application requires an evaluation of their effects on fungal secondary metabolism and membrane effects. In this study, different strains of Fusarium culmorum and F. graminearum sensu stricto were exposed to trans-cinnamic and chlorogenic acids on solid YES media. Fusaria produced phenolic acids, whose accumulation was lowered by exogenous phenolic compounds. In addition, fungi reduced exogenous phenolic acids, leading either to their conversion or degradation...
June 22, 2017: Toxins
https://www.readbyqxmd.com/read/28638985/glucuronidation-of-deoxynivalenol-don-by-different-animal-species-identification-of-iso-don-glucuronides-and-iso-deepoxy-don-glucuronides-as-novel-don-metabolites-in-pigs-rats-mice-and-cows
#18
Heidi E Schwartz-Zimmermann, Christian Hametner, Veronika Nagl, Iris Fiby, Lukas Macheiner, Janine Winkler, Sven Dänicke, Erica Clark, James J Pestka, Franz Berthiller
The Fusarium mycotoxin deoxynivalenol (DON) is a frequent contaminant of cereal-based food and feed. Mammals metabolize DON by conjugation to glucuronic acid (GlcAc), the extent and regioselectivity of which is species-dependent. So far, only DON-3-glucuronide (DON-3-GlcAc) and DON-15-GlcAc have been unequivocally identified as mammalian DON glucuronides, and DON-7-GlcAc has been proposed as further DON metabolite. In the present work, qualitative HPLC-MS/MS analysis of urine samples of animals treated with DON (rats: 2 mg/kg bw, single bolus, gavage; mice: 1 mg/kg bw, single i...
June 21, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28636459/inhibition-of-fusarium-solani-infection-in-murine-keratocytes-by-lactobacillus-salivarius-ssp-salivarius-jcm1231-culture-filtrate-in-vitro
#19
Jianzhang Hu, Fang Chen, Tong Kan, Hua Zhuang, Jingjin Zhang, Xiaoli Han
PURPOSE: To explore the inhibitory activity of Lactobacillus salivarius ssp. salivarius JCM1231 (L. salivarius JCM1231) culture filtrate against Fusarium solani (F. solani) and its effects on murine keratocytes (MKs) infected with F. solani. METHODS: L. salivarius JCM1231 was cultured in an anaerobic incubator for 24 h, and the L. salivarius culture filtrate (LSCF) was prepared .The antifungal activity of L. salivarius JCM1231 against F. solani was determined with a plate overlay assay, agar diffusion assay, and conidial germination inhibition test...
June 21, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28635676/comment-on-melanisation-of-aspergillus-terreus-is-butyrolactone-i-involved-in-the-regulation-of-both-dopa-and-dhn-types-of-pigments-in-submerged-culture-microorganisms-2017-5-22
#20
Elena Geib, Matthias Brock
A recent article by Palonen et al. describes the effect of butyrolactone I on the expression of a secondary metabolite biosynthesis gene cluster from Aspergillus terreus that shows similarities to fusarubin biosynthesis gene clusters from Fusarium species. The authors claim that two different types of pigments are formed in Aspergillus terreus conidia, whereby one pigment is termed a DOPA-type melanin and the second a DHN-type melanin. Unfortunately, the terminology of the classification of melanin-types requires revision as Asp-melanin present in A...
June 21, 2017: Microorganisms
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