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https://www.readbyqxmd.com/read/28086809/primary-mediastinal-clear-cell-sarcoma-a-case-report-and-review-of-the-literature
#1
Long Jin, Yuxia Sui, Haili Zhu, Zhizhong Chen, Shuguang Liu
BACKGROUND: Clear cell sarcoma (CCS) is a rare malignant soft-tissue neoplasm that displays melanocytic markers and exhibits striking histopathological features. The tumour has a predilection for the lower extremities and rarely presents in the mediastinum. CASE PRESENTATION: We present a case of primary mediastinal CCS in a 57-year-old man. Computer tomography (CT) revealed a 12 × 12 × 7.5 cm mass in the anterior mediastinum. Microscopically, the tumour mainly consisted of epithelioid cells with oval vesicular nuclei and eosinophilic cytoplasm...
January 13, 2017: Diagnostic Pathology
https://www.readbyqxmd.com/read/28081460/blood-brain-barrier-dysfunction-induced-by-silica-nps-in%C3%A2-vitro-and-in%C3%A2-vivo-involvement-of-oxidative-stress-and-rho-kinase-jnk-signaling-pathways
#2
Xin Liu, Baiyan Sui, Jiao Sun
Silica nanoparticles (SiO2-NPs) has been extensively exploited in biomedical fields and mostly designed to enter the circulatory system, however, few studies focused on the potential adverse effects of SiO2-NPs exposure on the blood-brain barrier (BBB) that serves as a critical barrier between the central nervous system (CNS) and the peripheral circulation. This study attempts to provide an understanding of whether and how SiO2-NPs disrupts the BBB in vitro and in vivo. Through a human BBB model, we found that SiO2-NPs could induce tight junction loss and cytoskeleton arrangement, and increase inflammatory response and the release of vascular endothelial growth factor (VEGF) of brain microvessel endothelial cells (BMECs), which further activates astrocytes to amplify the generation of VEGF and increase the aquaporin-4 expression, and thus causing BBB disruption through a complex immunoregulatory loop between BMECs and astrocytes under SiO2-NPs exposure...
January 4, 2017: Biomaterials
https://www.readbyqxmd.com/read/28081204/deletion-of-ssna-attenuates-the-pathogenicity-of-streptococcus-suis-and-confers-protection-against-serovar-2-strain-challenge
#3
Miao Li, Ru-Jian Cai, Chun-Ling Li, Shuai Song, Yan Li, Zhi-Yong Jiang, Dong-Xia Yang
Streptococcus suis serotype 2 (SS2) is a major porcine and human pathogen which causes arthritis, meningitis, and septicemia. Streptococcus suis nuclease A (SsnA) is a recently discovered deoxyribonuclease (DNase), which has been demonstrated to contribute to escape killing in neutrophil extracellular traps (NETs). To further determine the effects of ssnA on virulence, the ssnA deletion mutant (ΔssnA) and its complemented strain (C-ΔssnA) were constructed. The ability of ΔssnA mutant to interact with human laryngeal epithelial cell (Hep-2) was evaluated and it exhibited dramatically decreased ability to adhere to and invade Hep-2 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28080071/nanoscale-engineering-in-vo2-nanowires-via-direct-electron-writing-process
#4
Zhenhua Zhang, Hua Guo, Wenqiang Ding, Bin Zhang, Yue Lu, Xiaoxing Ke, Weiwei Liu, Fu-Rong Chen, Manling Sui
Controlling phase transition in functional materials at nano-scale is not only of broad scientific interest, but also important for practical applications in the fields of renewable energy, information storage, transducer, sensor, etc. As a model functional material, vanadium dioxide (VO2) has its metal-insulator transition (MIT) usually at a sharp temperature around 68 °C. Here we report a focused electron beam can directly lower down the transition temperature of a nano-area to room temperature without pre-patterning the VO2...
January 12, 2017: Nano Letters
https://www.readbyqxmd.com/read/28078483/theoretical-characterization-on-photoelectric-properties-of-benzothiadiazole-and-fluorene-based-small-molecule-acceptor-materials-for-the-organic-photovoltaics
#5
Mingyue Sui, Shuangbao Li, Qingqing Pan, Guangyan Sun, Yun Geng
The upper efficiency of heterojunction organic photovoltaics depends on the increased open-circuit voltage (V oc) and short-circuit current (J sc). So, a higher lowest unoccupied molecular orbital (LUMO) level is necessary for organic acceptor material to possess higher V oc and more photons absorbsorption in the solar spectrum is needed for larger J sc. In this article, we theoretically designed some small molecule acceptors (2∼5) based on fluorene (F), benzothiadiazole, and cyano group (CN) referring to the reported acceptor material 2-[{7-(9,9-di-n-propyl-9H-fluoren-2-yl)benzo[c][1,2,5]thiadiazol-4-yl}methylene]malononitrile (1), the crucial parameters affecting photoelectrical properties of compounds 2∼5 were evaluated by the density functional theory (DFT) and time dependent density functional theory (TDDFT) methods...
January 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28078081/occurrence-of-pasteurella-multocida-among-pigs-with-respiratory-disease-in-china-between-2011-and-2015
#6
Huisheng Liu, Zhanqin Zhao, Xiaojian Xi, Qiao Xue, Ta Long, Yun Xue
BACKGROUND: Prior to the 1990s, P. multocida capsular serogroup A was the most prevalent in China, followed by serogroups B and D. Thirty years later, serogroup D became the most prevalent, followed by serogroups A and B. However, the P. multocida capsular serogroups currently circulating in China remain unclear. Therefore, the aim of the present study was to provide an update on P. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in Central and Eastern China from 2011 to 2015...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/28077515/medial-prefrontal-cortex-pontine-nuclei-projections-modulate-suboptimal-cue-induced-associative-motor-learning
#7
Guang-Yan Wu, Shu-Lei Liu, Juan Yao, Lin Sun, Bing Wu, Yi Yang, Xuan Li, Qian-Quan Sun, Hua Feng, Jian-Feng Sui
Diverse and powerful mechanisms have evolved to enable organisms to modulate learning and memory under a variety of survival conditions. Cumulative evidence has shown that the prefrontal cortex (PFC) is closely involved in many higher-order cognitive functions. However, when and how the medial PFC (mPFC) modulates associative motor learning remains largely unknown. Here, we show that delay eyeblink conditioning (DEC) with the weak conditioned stimulus (wCS) but not the strong CS (sCS) elicited a significant increase in the levels of c-Fos expression in caudal mPFC...
January 10, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28076505/therapeutic-effect-of-low-molecular-weight-chitosan-containing-sepia-ink-on-ethanol-induced-gastric-ulcer-in-rats
#8
Wei Zhang, Kang Liu, Lei Li, Yingxin Li, Xianxian Sui, Yinzhu Rao, Jiahao Wu, Qiuping Wu
PURPOSE: To evaluate the role of low molecular chitosan containing sepia ink (LMCS) in ethanol-induced (5 ml/kg) gastric ulcer in rats. METHODS: Animals were divided into four groups (n = 12): normal group (Normal), negative control group (Con), experiment group (LMCS) and positive control Omeprazole group (OMZ). Gastric empty rate was detected in the first 7 days. Rats were sacrificed at 7, 14 and 21 day for histology and ELISA detections. RESULTS: Gastric empty was no significant differences among the groups (P > 0...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28071019/influence-of-different-extraction-conditions-on-antioxidant-properties-of-soursop-peel
#9
Wei Zin Lee, Sui Kiat Chang, Hock Eng Khoo, Chiaw Mei Sia, Hip Seng Yim
BACKGROUND: Soursop is a healthy fruit. Peels form about 20% of the soursop fruit and are usually discarded as waste product. With a view to utilizing soursop peel as a source of valuable compounds, this study aimed to investigate the influence of different extraction conditions on total phenolic content (TPC) and antioxidant capacity (AC) of soursop (Annona muricata L.) peel. METHODS: Different ethanol concentrations (20-100%, v/v), extraction temperatures (25- 60°C), and extraction time (1-5 h) were tested...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28070076/quantitative-determination-of-total-amino-acids-based-on-surface-enhanced-raman-scattering-and-ninhydrin-derivatization
#10
Huimin Sui, Lei Chen, Xiao Xia Han, Xiaolei Zhang, Xiaolei Wang, Bing Zhao
In the present study, we propose a simple and sensitive method for the determination of total amino acids without any separation steps. The procedure described here is based on the ninhydrin derivatization reaction with amino acids, followed by surface-enhanced Raman scattering (SERS) measurements of the producing mixtures. A good linear correlation of excess ninhydrin SERS signals and the log values of the total amino acids concentrations is obtained; the detection limit of the method is 4.3 × 10(-9) mol L(-1)...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28070044/-expression-of-lactate-dehydrogenase-5-in-non-hodgkin-lymphoma-and-its-clinical-significance
#11
Fei Dong, Aixia Sui, Hongmei Jing
To determine expression of lactate dehydrogenase (LDH)-5 in non-Hodgkin lymphoma and its clinical significance.
 Methods: LDH-5 levels and LDH levels in NHL patients were examined by agarose gel electrophoresis and enzymatic method (n=63), respectively. Positive rates of LDH-5 and LDH were statistically analyzed.
 Results: The median age of NHL patients was 56(19-84) years old, including 36 males and 27 females. The positive numbers for LDH-5 and LDH in the initial treatment group (n=43) were significantly different (P<0...
December 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28068884/evaluation-of-protein-spectra-cluster-analysis-for-streptococcus-spp-identification-from-various-swine-clinical-samples
#12
Carlos E C Matajira, Luisa Z Moreno, Vasco T M Gomes, Ana Paula S Silva, Renan E Mesquita, Daniela S Doto, Franco F Calderaro, Fernando N de Souza, Ana Paula G Christ, Maria Inês Z Sato, Andrea M Moreno
Traditional microbiological methods enable genus-level identification of Streptococcus spp. isolates. However, as the species of this genus show broad phenotypic variation, species-level identification or even differentiation within the genus is difficult. Herein we report the evaluation of protein spectra cluster analysis for the identification of Streptococcus species associated with disease in swine by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 250 S...
January 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28066979/when-proteostasis-goes-bad-protein-aggregation-in-the-cell
#13
REVIEW
Mona Radwan, Rebecca J Wood, Xiaojing Sui, Danny M Hatters
Protein aggregation is a hallmark of the major neurodegenerative diseases including Alzheimer's, Parkinson's, Huntington's and motor neuron and is a symptom of a breakdown in the management of proteome foldedness. Indeed, it is remarkable that under normal conditions cells can keep their proteome in a highly crowded and confined space without uncontrollable aggregation. Proteins pose a particular challenge relative to other classes of biomolecules because upon synthesis they must typically follow a complex folding pathway to reach their functional conformation (native state)...
January 9, 2017: IUBMB Life
https://www.readbyqxmd.com/read/28064143/production-of-trihalomethanes-haloacetaldehydes-and-haloacetonitriles-during-chlorination-of-microcystin-lr-and-impacts-of-pre-oxidation-on-their-formation
#14
Wenhai Chu, Dechang Yao, Yang Deng, Minghao Sui, Naiyun Gao
Microcystins (MCs) in drinking water have gained much attention due to their adverse health effects. However, little is known about the impact of pre-oxidation in the formation of disinfection by-products (DBPs) during the downstream chlorination of MCs. The present study examined the formation of both carbonaceous and nitrogenous DBPs from chlorination of MC-LR (the most abundant MC species) and evaluated the impact of permanganate (PM), hydrogen peroxide (H2O2) and chlorine dioxide (ClO2) pre-oxidation on the DBP formation in chlorination...
December 29, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28061848/multi-antigen-avian-influenza-a-h7n9-virus-like-particles-particulate-characterizations-and-immunogenicity-evaluation-in-murine-and-avian-models
#15
Che-Ming Jack Hu, Chu-Yang Chien, Ming-Tsan Liu, Zih-Syun Fang, Sui-Yuan Chang, Rong-Huay Juang, Shih-Chung Chang, Hui-Wen Chen
BACKGROUND: Human infection with avian influenza A virus (H7N9) was first reported in China in March 2013. Since then, hundreds of cases have been confirmed showing severe symptoms with a high mortality rate. The virus was transmitted from avian species to humans and has spread to many neighboring areas, raising serious concerns over its pandemic potential. Towards containing the disease, the goal of this study is to prepare a virus-like particle (VLP) that consists of hemagglutinin (HA), neuraminidase (NA) and matrix protein 1 (M1) derived from the human isolate A/Taiwan/S02076/2013(H7N9) for potential vaccine development...
January 7, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28061775/immunization-with-streptococcus-suis-bacterin-plus-recombinant-sao-protein-in-sows-conveys-passive-immunity-to-their-piglets
#16
Kai-Jen Hsueh, Li-Ting Cheng, Jai-Wei Lee, Yao-Chi Chung, Wen-Bin Chung, Chun-Yen Chu
BACKGROUND: Streptococcus suis (S. suis) causes arthritis, meningitis, septicemia, and sudden death in pigs and is also an zoonotic agent for humans. The present study demonstrated that immunization with recombinant Sao-L (surface antigen one-L, rSao-L) protein from a strain of S. suis serotype 2 in pigs was able to increase cross-serotype protection against S. suis serotype 1 and 2 challenge. Since weaning piglets are more susceptible to S. suis infections due to the stresses associated with weaning, prepartum immunization in sows may convey passive immunity to piglets and provide protection...
January 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28061477/autocrine-vegf-signaling-promotes-cell-proliferation-through-a-plc-dependent-pathway-and-modulates-apatinib-treatment-efficacy-in-gastric-cancer
#17
Yi Lin, Ertao Zhai, Bing Liao, Lixia Xu, Xinhua Zhang, Sui Peng, Yulong He, Shirong Cai, Zhirong Zeng, Minhu Chen
BACKGROUND: Tumor cells produce vascular endothelial growth factor (VEGF) which interact with the membrane or cytoplasmic VEGF receptors (VEGFRs) to promote cell growth in an angiogenesis-independent fashion. Apatinib, a highly selective VEGFR2 inhibitor, is the only effective drug for patients with terminal gastric cancer (GC) who have no other chemotherapeutic options. However, its treatment efficacy is still controversy and the mechanism behind remains undetermined. In this study, we aimed to investigate the role of autocrine VEGF signaling in the growth of gastric cancer cells and the efficacy of Apatinib treatment...
January 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28058577/development-of-an-indirect-dot-ppa-elisa-using-glutamate-dehydrogenase-as-a-diagnostic-antigen-for-the-rapid-and-specific-detection-of-streptococcus-suis-and-its-application-to-clinical-specimens
#18
Xiao-Jing Xia, Lei Wang, Zhi-Qiang Shen, Wanhai Qin, Jianhe Hu, Shi-Jin Jiang, Shu-Guang Li
Streptococcus suis is an important zoonotic pathogen causing infections in pigs and humans. Bacterial surface-related proteins are often explored as potential vaccine candidates and diagnostic antigens. In the present study, glutamate dehydrogenase, a highly conserved immunogenic extracellular protein, was used to establish a dot horseradish peroxidase enzyme-linked staphylococcal protein A immunosorbent assay (Dot-PPA-ELISA) for diagnosis of S. suis infection. The antigen-antibody reaction was optimised through checkerboard titration involving serial dilutions, followed by selective blocking tests and evaluations of cross-reaction, repeatability, and stability...
January 5, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28057904/a-serine-threonine-phosphatase-1-of-streptococcus-suis-type-2-is-an-important-virulence-factor
#19
Lihua Fang, Pengcheng Fan, Yunkai Yang, Jingjing Zhou, Hongxia Shen, Weihuan Fang
Streptococcus suis is regarded as one of the major pathogens of pigs while S. suis type 2 (SS2) is considered to be a zoonotic bacterium for its ability to cause meningitis and streptococcal toxic shock-like syndrome in humans. Many bacterial species contain genes encoding serine/threonine protein phosphatases (STP) responsible for dephosphorylation of their substrates in a single reaction step. This study was aimed to investigate the role of stp1 in pathogenesis of SS2. An isogenic stp1 mutant (Δstp1) was constructed from the SS2 strain ZJ081101...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28057111/-health-economic-evaluation-of-a-23-value-pneumococcal-polysaccharide-vaccination-pilot-programme-among-elderly-chronic-obstructive-pulmonary-disease-patients-in-china
#20
Y P Qiu, K Zhao, X Li, L W Shi, W D Guo, X R Qi, B Y Sui, R M Zhou
Objective: From the perspective of health economics, to evaluate 23 pneumococcal polysaccharide vaccination programme among chronic obstructive pulmonary disease (COPD) patient. Methods: In the pilot counties of the project of integrated care pathway for COPD patient (Hanbin district of Hanzhong city in Shanxi Province, Qianjian district of Qingqing city, Huandao district of Qindao city in Shangdong Province, Wen county of Jiaozuo city in Henan Province), information of insurance participants of New Rural Cooperative Medical System (NRCS) was collected by local NRCM information system, which included general information as well as records of medical care and medical fee...
December 6, 2016: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
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