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https://www.readbyqxmd.com/read/28812572/population-characteristics-of-a-large-whale-shark-aggregation-inferred-from-seawater-environmental-dna
#1
Eva Egelyng Sigsgaard, Ida Broman Nielsen, Steffen Sanvig Bach, Eline D Lorenzen, David Philip Robinson, Steen Wilhelm Knudsen, Mikkel Winther Pedersen, Mohammed Al Jaidah, Ludovic Orlando, Eske Willerslev, Peter Rask Møller, Philip Francis Thomsen
Population genetics is essential for understanding and managing marine ecosystems, but sampling remains challenging. We demonstrate that high-throughput sequencing of seawater environmental DNA can provide useful estimates of genetic diversity in a whale shark (Rhincodon typus) aggregation. We recover similar mitochondrial haplotype frequencies in seawater compared to tissue samples, reliably placing the studied aggregation in a global genetic context and expanding the applications of environmental DNA to encompass population genetics of aquatic organisms...
November 21, 2016: Nature ecology & evolution
https://www.readbyqxmd.com/read/28812085/high-tc-superconducting-phases-in-organic-molecular-intercalated-iron-selenides-synthesis-and-crystal-structures
#2
Shifeng Jin, Xiao Fan, Xiaozhi Wu, Ruijin Sun, Hui Wu, Qingzhen Huang, Chenlong Shi, Xuekui Xi, Zhilin Li, Xiaolong Chen
Hybrid iron-based superconductors were synthesized by sonochemical insertion of organic molecules into FeSe layers. High quality of the samples first enabled reliable phase identifications and three structure types were discovered. Structure determination based on neutron data further facilitated the understanding of their structural stability, doping levels and temperature driven structural transitions.
August 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28809829/horizontal-whole-mount-a-novel-processing-and-imaging-protocol-for-thick-three-dimensional-tissue-cross-sections-of-skin
#3
Lucia Salz, Ryan R Driskell
Processing a tissue of interest to generate a microscopic image that supports a scientific argument can be challenging. The acquisition of high-quality microscopic images is not entirely dependent upon the quality of the microscope, but also upon the methods of tissue processing, which often involve multiple critical actions or steps. Furthermore, mesenchymal cell types in the skin and other tissues represent a new challenge for tissue preparation and imaging. Here, we present a complete process, from tissue harvest to microscopy...
August 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28808091/inter-rater-reliability-and-downstream-financial-implications-of-electrocardiography-screening-in-young-athletes
#4
Harshil Dhutia, Aneil Malhotra, Tee Joo Yeo, Irina Chis Ster, Vincent Gabus, Alexandros Steriotis, Helder Dores, Greg Mellor, Carmen García-Corrales, Bode Ensam, Viknesh Jayalapan, Vivienne Anne Ezzat, Gherardo Finocchiaro, Sabiha Gati, Michael Papadakis, Maria Tome-Esteban, Sanjay Sharma
BACKGROUND: Preparticipation screening for cardiovascular disease in young athletes with electrocardiography is endorsed by the European Society of Cardiology and several major sporting organizations. One of the concerns of the ECG as a screening test in young athletes relates to the potential for variation in interpretation. We investigated the degree of variation in ECG interpretation in athletes and its financial impact among cardiologists of differing experience. METHODS AND RESULTS: Eight cardiologists (4 with experience in screening athletes) each reported 400 ECGs of consecutively screened young athletes according to the 2010 European Society of Cardiology recommendations, Seattle criteria, and refined criteria...
August 2017: Circulation. Cardiovascular Quality and Outcomes
https://www.readbyqxmd.com/read/28807812/evaluation-and-clinical-validation-of-two-field-deployable-reverse-transcription-insulated-isothermal-pcr-assays-for-the-detection-of-the-middle-east-respiratory-syndrome-coronavirus
#5
Yun Young Go, Yeon-Sook Kim, Shinhye Cheon, Sangwoo Nam, Keun Bon Ku, Meehyein Kim, Nam Hyuk Cho, Hyun Park, Pei-Yu Alison Lee, Yu-Chun Lin, Yun-Long Tsai, Hwa-Tang Thomas Wang, Udeni B R Balasuriya
Middle East respiratory syndrome (MERS) is an emerging zoonotic viral respiratory disease that was first identified in Saudi Arabia in 2012. In 2015, the largest MERS outbreak outside of the Middle East region occurred in the Republic of Korea. The rapid nosocomial transmission of MERS-coronavirus (MERS-CoV) in Korean healthcare settings highlighted the importance and urgent need for a rapid and reliable on-site diagnostic assay to implement effective control and preventive measures. Here, we describe the evaluation and validation of two newly developed reverse transcription-insulated isothermal PCR (RT-iiPCR) methods targeting the ORF1a and upE genes of MERS-CoV...
August 11, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28800693/a-modified-sintacs-method-for-groundwater-vulnerability-and-pollution-risk-assessment-in-highly-anthropized-regions-based-on-no3-and-so4-2-concentrations
#6
Busico Gianluigi, Kazakis Nerantzis, Colombani Nicolò, Mastrocicco Micòl, Voudouris Konstantinos, Tedesco Dario
Groundwater vulnerability and risk assessment area worldwide tools in supporting groundwater protection and land planning. In this study, we used three of these different methodologies applied to the Campanian Plain located in southern Italy: SINTACS, AVI and LOS. However, their capability to describe the observed chemical pollution of the area has resulted quite poor. For such a reason, a modified SINTACS method has been then implemented in the area in order to get a more reliable view of groundwater vulnerability...
August 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28797523/the-acute-toxic-effects-of-1-alkyl-3-methylimidazolium-nitrate-ionic-liquids-on-chlorella-vulgaris-and-daphnia-magna
#7
Cheng Zhang, Shuai Zhang, Lusheng Zhu, Jinhua Wang, Jun Wang, Tongtong Zhou
Given their increasingly widespread application, the toxic effects of ionic liquids (ILs) have become the subject of significant attention in recent years. Therefore, the present study assessed the acute toxic effects of 1-alkyl-3-methylimidazolium nitrate ([Cnmim]NO3 (n = 2, 4, 6, 8, 10, 12)) on Chlorella vulgaris and Daphnia magna. The sensitivity of the tested organism Daphnia magna and the investigated IL concentrations in water using high-performance liquid chromatography (HPLC) were also evaluated to demonstrate the reliability of the present study...
August 7, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28795166/an-efficient-strategy-using-k-mers-to-analyse-16s-rrna-sequences
#8
Marcel Martínez-Porchas, Francisco Vargas-Albores
The use of k-mers has been a successful strategy for improving metagenomics studies, including taxonomic classifications, or de novo assemblies, and can be used to obtain sequences of interest from the available databases. The aim of this manuscript was to propose a simple but efficient strategy to generate k-mers and to use them to obtain and analyse in silico 16S rRNA sequence fragments. A total of 513,309 bacterial sequences contained in the SILVA database were considered for the study, and homemade PHP scripts were used to search for specific nucleotide chains, recover fragments of bacterial sequences, make calculations and organize information...
July 2017: Heliyon
https://www.readbyqxmd.com/read/28792274/global-regional-and-national-prevalence-incidence-and-disability-adjusted-life-years-for-oral-conditions-for-195-countries-1990-2015-a-systematic-analysis-for-the-global-burden-of-diseases-injuries-and-risk-factors
#9
N J Kassebaum, A G C Smith, E Bernabé, T D Fleming, A E Reynolds, T Vos, C J L Murray, W Marcenes
The Global Burden of Disease 2015 study aims to use all available data of sufficient quality to generate reliable and valid prevalence, incidence, and disability-adjusted life year (DALY) estimates of oral conditions for the period of 1990 to 2015. Since death as a direct result of oral diseases is rare, DALY estimates were based on years lived with disability, which are estimated only on those persons with unmet need for dental care. We used our data to assess progress toward the Federation Dental International, World Health Organization, and International Association for Dental Research's oral health goals of reducing the level of oral diseases and minimizing their impact by 2020...
April 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28789788/alexithymia-and-anesthetic-bladder-capacity-in-interstitial-cystitis-bladder-pain-syndrome
#10
Chui-De Chiu, Ming-Huei Lee, Wei-Chih Chen, Hoi Lam Ho, Huei-Ching Wu
OBJECTIVE: In contrast to the inconsistent results of organic causes, it has been found that psychological risk factors are reliably related to functional somatic syndromes (FSSs), including interstitial cystitis/bladder pain syndrome (IC/BPS). Compared to patients with acute cystitis, a subgroup of IC/BPS patients with a history of childhood relational trauma reported intensified unregulated affective states (i.e., anxiety and depression) and trauma-related psychopathology (i.e., dissociation)...
September 2017: Journal of Psychosomatic Research
https://www.readbyqxmd.com/read/28783915/comparison-of-pocis-passive-samplers-vs-composite-water-sampling-a-case-study
#11
Justine Criquet, David Dumoulin, Michael Howsam, Leslie Mondamert, Jean-François Goossens, Jean Prygiel, Gabriel Billon
The relevance of Polar Organic Chemical Integrative Samplers (POCIS) was evaluated for the assessment of concentrations of 46 pesticides and 19 pharmaceuticals in a small, peri-urban river with multi-origin inputs. Throughout the period of POCIS deployment, 24h-average water samples were collected automatically, and showed the rapid temporal evolution of concentrations of several micropollutants, as well as permitting the calculation of average concentrations in the water phase for comparison with those estimated from POCIS passive samplers...
August 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28776982/flux-analysis-of-free-amino-sugars-and-amino-acids-in-soils-by-isotope-tracing-with-a-novel-liquid-chromatography-high-resolution-mass-spectrometry-platform
#12
Yuntao Hu, Qing Zheng, Wolfgang Wanek
Soil fluxomics analysis can provide pivotal information for understanding diverse soil biochemical pathways and their regulation, but direct measurement methods are very rare. In this work, we describe an approach to measure soil extracellular metabolite (amino sugar and amino acid) fluxes based on the (15)N isotope pool dilution technique via liquid chromatography and high resolution mass spectrometry (LC/HRMS). We produced commercially unavailable (15)N and (13)C labeled amino sugars and amino acids by hydrolyzing peptidoglycan isolated from isotopically labeled bacterial biomass, and used them as tracers ((15)N) and internal standards ((13)C)...
August 4, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28776899/an-alternative-sterility-assessment-for-parenteral-drug-products-using-isothermal-microcalorimetry
#13
D Brueckner, S Krähenbühl, U Zuber, G Bonkat, O Braissant
AIMS: Production and release of injectable drug solutions are highly regulated since the administration of injectables bypasses natural body barriers. The sterility test is the last opportunity of product quality assessment. However, sterility is currently assessed by visual inspection (VI) that is time consuming and somewhat subjective. Therefore, we assessed isothermal microcalorimetry (IMC) as a replacement for the VI of the filtration based state-of-the-art sterility control. METHODS AND RESULTS: We used ATCC strains and house isolates to artificially contaminate frequently produced monoclonal antibodies (Avastin, Mabthera, Herceptin)...
August 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28773891/flow-chamber-system-for-the-statistical-evaluation-of-bacterial-colonization-on-materials
#14
Friederike Menzel, Bianca Conradi, Karsten Rodenacker, Anna A Gorbushina, Karin Schwibbert
Biofilm formation on materials leads to high costs in industrial processes, as well as in medical applications. This fact has stimulated interest in the development of new materials with improved surfaces to reduce bacterial colonization. Standardized tests relying on statistical evidence are indispensable to evaluate the quality and safety of these new materials. We describe here a flow chamber system for biofilm cultivation under controlled conditions with a total capacity for testing up to 32 samples in parallel...
September 10, 2016: Materials
https://www.readbyqxmd.com/read/28767284/commutability-of-reference-materials-for-%C3%AE-fetoprotein-in-human-serum
#15
Yuhong Yue, Shunli Zhang, Zhenzhen Xu, Xi Chen, Qingtao Wang
CONTEXT: - Reliable quantification of α-fetoprotein (AFP) is critical for clinical diagnosis. Accuracy in AFP analysis relies on traceability to reference materials with confirmed commutability. OBJECTIVE: - To assess the commutability of the reference materials for AFP. We screened for appropriate reference materials for the calibration of clinical AFP analysis and for application in an external quality assessment scheme. The feasibility of using water to dilute a reference material from the World Health Organization was also evaluated...
August 2, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28766682/fasr-and-fasl-in-colorectal-cancer
#16
Magdalena Szarynska, Agata Olejniczak, Piotr Wierzbicki, Jaroslaw Kobiela, Dariusz Laski, Zbigniew Sledzinski, Krystian Adrych, Marek Guzek, Zbigniew Kmiec
Colorectal cancer (CRC) is one of the most common solid organ cancers prevalent worldwide causing, in spite of advancing therapeutic methodology, high rate of patient mortality, especially due to metastasis development. The cancer stem cell (CSC) theory of tumor growth indicates that CSCs within the tumor mass have great capacity to initiate and sustain tumor growth. Following the suggestion that Fas signaling can be engaged in apoptosis, tumor maintenance, senescence or DICE (death induced by CD95 or CD95L elimination), the attempts to broaden the knowledge concerning the relationships between CSCs features and FasR/FasL appeared to be necessary...
September 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28764714/testis-specific-histone-2b-is-associated-with-sperm-chromatin-dynamics-and-bull-fertility-a-pilot-study
#17
Naseer A Kutchy, Ana Velho, Erika S B Menezes, Marie Jacobsen, Giselle Thibaudeau, Robert W Wills, Arlindo Moura, Abdullah Kaya, Andy Perkins, Erdogan Memili
BACKGROUND: Bull fertility is the degree of sperm's ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is similar to those of other mammals including humans. Moreover, reliable fertility data along with well-established in vitro systems are available for bovine. The objective of this original study was to ascertain evolutionary diversification and expression dynamics of Testis Specific Histone 2B (TH2B) in sperm from Holstein bulls with different fertility scores...
August 1, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28764711/video-tactile-pneumatic-sensor-for-soft-tissue-elastic-modulus-estimation
#18
M M Gubenko, A V Morozov, A N Lyubicheva, I G Goryacheva, M Z Dosaev, M-Sh Ju, Ch-H Yeh, F-Ch Su
BACKGROUND: A new sensor for estimating elasticity of soft tissues such as a liver was developed for minimally invasive surgery application. METHODS: By measuring deformation and adjusting internal pressure of the pneumatic sensor head, the sensor can be used to do palpation (indentation) of tissues with wide range of stiffness. A video camera installed within the sensor shell is used to register the radius of the contact area. Based on finite element model simulations and the measured data, elastic modulus of the indented soft tissue can be calculated...
August 1, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28759437/modified-adm1-for-high-rate-anaerobic-co-digestion-of-thermally-pre-treated-brewery-surplus-yeast-wastewater
#19
Liam Pettigrew, Alexander Gutbrod, Herbert Domes, Frauke Groß, Juan M Méndez-Contreras, Antonio Delgado
Co-digestion of surplus yeast with brewery wastewater is a potentially economical method for recovering energy, in the form of biogas, from this difficult to dispose of by-product. In this work a modified version of the ADM1 (Anaerobic Digestion Model No. 1) was calibrated for an anaerobic digester fed with thermally pre-treated brewery yeast surplus wastewater. The model could predict changes to reactor methane production and reduction of biodegradable matter when fed with both pre-treated and untreated yeast surplus wastewater at varying loading rates...
July 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28757679/acoustic-ranging-in-poison-frogs-it-is-not-about-signal-amplitude-alone
#20
Max Ringler, Georgine Szipl, Walter Hödl, Leander Khil, Barbara Kofler, Michael Lonauer, Christina Provin, Eva Ringler
ABSTRACT: Acoustic ranging allows identifying the distance of a sound source and mediates inter-individual spacing and aggression in territorial species. Birds and mammals are known to use more complex cues than only sound pressure level (SPL), which can be influenced by the signaller and signal transmission in non-predictable ways and thus is not reliable by itself. For frogs, only SPL is currently known to mediate inter-individual distances, but we hypothesise that the strong territoriality of Dendrobatids could make the use of complex cues for ranging highly beneficial for this family...
2017: Behavioral Ecology and Sociobiology
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