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https://www.readbyqxmd.com/read/28221022/quantitative-13c-traces-of-glucose-fate-in-hepatitis-b-virus-infected-hepatocytes
#1
Qianfen Wan, Yulan Wang, Huiru Tang
Quantitative characterization of 13C-labeled metabolites is an important part of the stable isotope tracing method widely used in metabolic flux analysis. Due to long relaxation time and low sensitivity of 13C nuclei, direct measurement of 13C labeled metabolites using one dimensional 13C NMR often fails to meet the demand of metabolomics studies especially with large number of samples and metabolites having low abundance. Although HSQC-based 2D NMR methods have improved sensitivity with inversion detection, they are time-consuming thus unsuitable for high-throughput absolute quantification of 13C-labeled metabolites...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28216877/using-uhplc-and-uv-vis-fingerprint-method-to-evaluate-substitutes-for-swertia-mileensis-an-endangered-medicinal-plant
#2
Jie Li, Ji Zhang, Hang Jin, Yuan-Zhong Wang, Heng-Yu Huang
BACKGROUND: Millions of people are killed by viral hepatitis every year in the world, whereas many relevant medicines are too expensive to purchase. Swertia mileensis, a medicinal plant for hepatitis in the system of traditional Chinese medicine, has been vanishing gradually because of overexploitation. OBJECTIVE: To find substitutes of S. mileensis and reduce the cost of purchasing drugs for hepatitis patients, the similarity of phytochemical constituents between S...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28216372/hepatitis-b-virus-xprotein-stimulates-high-mobility-group-box-1-secretion-and-enhances-hepatocellular-carcinoma-metastasis
#3
Shuzhen Chen, Zihui Dong, Pinghua Yang, Xianming Wang, Guangzhi Jin, Han Yu, Lei Chen, Liang Li, Liang Tang, Shilei Bai, Hexin Yan, Feng Shen, Wenming Cong, Wen Wen, Hongyang Wang
: Hepatitis B virus X protein (HBx) plays an important role in the progression of hepatocellular carcinoma. Here we reported that overexpression of HBx in hepatocellular carcinoma (HCC) cells could induce the secretion of high-mobility group box 1 (HMGB1) to promote invasion and metastasis of HCC in an autocrine/paracrine manner. HBx triggered an increase of cytoplasmic calcium and activated CAMKK/CAMKIV pathway, leading to subsequent translocation and release of HMGB1. HMGB1 neutralizing antibody, as well as calcium chelator or inhibitors of CAMKK/CAMKIV, could remarkably reduce invasion and metastasis of HCC cells in vitro and in a murine HCC metastasis model in vivo...
February 16, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28214886/adenovirus-vector-harboring-the-hbcag-and-tripeptidyl-peptidase-ii-genes-induces-potent-cellular-immune-responses-in-vivo
#4
Quanhui Tan, Siyuan Ma, Jianjun Hu, Xiaohua Chen, Yongsheng Yu, Zhenghao Tang, Guoqin Zang
BACKGROUND: Chronic hepatitis B virus (HBV) infection is associated with a weak but specific cellular immune response of the host to HBV. Tripeptidyl peptidaseⅡ (TPPⅡ), an intracellular macromolecule and proteolytic enzyme, plays an important complementary and compensatory role for the proteasome during viral protein degradation and major histocompatibility complex class I antigen presentation by inducing a specific cellular immune response in vivo. Based on a previous study, we aimed to explore the role of MHC class I antigen presentation in vivo and the mechanisms that may be involved...
January 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28213165/hbvcircle-a-novel-tool-to-investigate-hepatitis-b-virus-covalently-closed-circular-dna
#5
Zhipeng Yan, Jing Zeng, Youjun Yu, Kunlun Xiang, Hui Hu, Xue Zhou, Lili Gu, Li Wang, Jie Zhao, John A T Young, Lu Gao
BACKGROUND & AIMS: Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) persists as a stable episome in infected hepatocytes and serves as a template for the transcription of all viral genes. Due to the narrow host range of HBV, the development of a robust mouse model that supports cccDNA-dependent viral replication is a key hurdle in the development of novel HBV therapeutics. This study aimed to develop a novel tool to investigate HBV cccDNA. METHODS: Through minicircle technology, HBVcircle, a recombinant cccDNA, was easily generated and extracted from a genetically engineered E...
February 14, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28209818/complete-genome-sequence-of-the-who-international-standard-for-hepatitis-b-virus-dna
#6
Adrian Jenkins, Rehan Minhas, Clare Morris, Neil Berry
The World Health Organization (WHO) international standard (IS) for hepatitis B virus (HBV) DNA for use in nucleic acid amplification assays was characterized by determining the complete genome sequence, which was assigned genotype A. This information will aid the design, development, and evaluation of HBV DNA amplification assays.
February 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28207518/virological-and-serological-features-of-acute-hepatitis-b-in-adults
#7
Xiaofei Du, Yali Liu, Lina Ma, Junfeng Lu, Yi Jin, Shan Ren, Zhimin He, Xinyue Chen
Various viral kinetics among patients with acute hepatitis B (AHB) have been observed in clinical practice. This study investigated the virological, biochemical, and serological characteristics of AHB in adults.A total of 192 adult patients with AHB were recruited between December 2010 and January 2014. The quantification of biochemical and serologic markers for hepatitis B virus (HBV) infection was monitored from the onset.Of the 192 patients, 113 patients were followed up. One patient died due to acute liver failure, 2 developed chronic HBV infection...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28207324/is-serum-angiotensin-converting-enzyme-level-useful-for-determining-necroinflammatory-activity-in-chronic-hepatitis-b-infection
#8
Ahmet Tay, Fatih Albayrak, Sevilay Ozmen, Ayse Albayrak, Kemalettin Ozden
AIM: The purpose of this study was to investigate the relationship between the findings from liver biopsy and the serum angiotensin-converting enzyme (ACE) level to determine whether ACE might serve as a potential noninvasive sign of necroinflammatory activity in patients with Chronic Hepatitis B (CHB) infection. METHODS: A total of 54 CHB patients referred for liver biopsy were enrolled in the study. Serum ACE levels were determined photometrically with a kinetic test...
February 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28206681/hepatitis-b-reactivation-in-patients-with-chronic-hepatitis-c-undergoing-anti-viral-therapy-with-an-interferon-free-regimen
#9
M-C Londoño, S Lens, Z Mariño, M Bonacci, X Ariza, T Broquetas, A Pla, C Bartres, M V Adriani, S Rodríguez-Tajes, J Costa, J A Carrión, S Pérez-Del-Pulgar, X Forns
BACKGROUND: A few cases of hepatitis B virus (HBV) reactivation during anti-viral therapy against hepatitis C (HCV) have been reported. However, the information regarding the real impact of this phenomenon is scarce. AIM: To evaluate the risk of HBV reactivation during anti-viral therapy against HCV with an interferon-free regimen with direct-acting anti-virals (DAAs). METHODS: Observational and prospective study of 352 patients receiving DAAs therapy between September 2015 and May 2016...
February 16, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28202793/the-new-aptima-hbv-quant-real-time-tma-assay-accurately-quantifies-hepatitis-b-virus-dna-from-genotypes-a-to-f
#10
Stéphane Chevaliez, Claude Dauvillier, Fabienne Dubernet, Jean-Dominique Poveda, Syria Laperche, Christophe Hézode, Jean-Michel Pawlotsky
Sensitive and accurate hepatitis B virus (HBV) DNA detection and quantification are essential to diagnose HBV infection, establish the prognosis of HBV-related liver disease, guide the decision to treat and monitor the virological response to antiviral treatment and the emergence of resistance. Currently available HBV DNA platforms and assays are generally designed for batching multiple specimens within an individual run and require at least one full day of work to complete the analyses. The aim of this study was to evaluate the ability of the newly developed, fully automated, one-step Aptima HBV Quant assay to accurately detect and quantify HBV DNA in a large series of patients infected with different HBV genotypes...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28199772/a-retrospective-study-of-hepatitis-b-mother-to-child-transmission-prevention-and-post-vaccination-serological-test-results-of-infants-at-risk-of-perinatal-transmission-in-two-counties-of-middle-china
#11
Guo Yonghao, Dong Pumei, Yang Jianhui, Xu Jin, Zhang Yanyang, Wang Zhe
Approximately 240 million people have chronic hepatitis B worldwide, with mother-to-child-transmission (MTCT) accounting for most cases. Therefore, Henan Province, China launched a public health program to prevent MTCT. To determine the efficacy of this health program, a survey was carried out in Huixian and Xinan counties, which are located in northern and western Henan. All infants born in these two counties between January 1, 2013 and March 31, 2014 to a mother positive for hepatitis B surface antigen (HBsAg), were surveyed; in total, there were 438 mother-infant pairs...
February 15, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28199034/hepatitis-b-surface-antigen-reduction-by-switching-from-long-term-nucleoside-nucleotide-analog-administration-to-pegylated-interferon
#12
Nobuharu Tamaki, Masayuki Kurosaki, Atsunori Kusakabe, Etsuro Orito, Kouji Joko, Yuji Kojima, Hiroyuki Kimura, Yasushi Uchida, Chitomi Hasebe, Yasuhiro Asahina, Namiki Izumi
Hepatitis B surface antigen (HBsAg) reduction during nucleoside/nucleotide analog (NA) therapy is slow and an alternative strategy for patients receiving ongoing NA to facilitate HBsAg reduction is required. We investigated whether switching to pegylated interferon (PEG-IFN) after long-term NA administration enhances HBsAg reduction. Forty-nine patients who switched from long-term NA to 48 weeks of PEG-IFN alfa-2a were studied. The mean duration of previous NA was 48 months (sequential group). A total of 147 patients who continued NA and matched for baseline characteristics were analyzed for comparison (NA continuation group)...
February 15, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28198546/a-molecular-epidemiology-study-investigating-familial-clustering-of-hepatitis-b-virus-infection-in-families-with-unfavorable-prognoses-in-northwest-china
#13
Yuan Yang, Dan Du, Li Jin, Zhen Tian, Qian Li, Ruitian Yi, Ting Qiu, Daokun Yang, Yingli He, Jinfeng Liu, Tianyan Chen, Yingren Zhao
Hepatitis B virus (HBV) infections and adverse outcome have been demonstrated to show characteristics of familial clustering. The aim of this study was to investigate the prevalence of different HBV genotypes, HBV sub-genotypes, and Pre-S mutations associated with familial HBV infection clusters with unfavorable prognoses. Families presenting with clustered HBV infections and unfavorable prognoses were enrolled in this study. Non-clustered HBV-infected individuals were used as the control group. DNA extracted from patient serum samples was used to facilitate characterization of the HBV genotypes, HBV sub-genotypes, and Pre-S mutations by phylogenetic analysis...
February 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28196636/impact-of-schistosomiasis-on-increase-incidence-of-occult-hepatitis-b-in-chronic-hepatitis-c-patients-in-egypt
#14
Hanan H Omar, Samaa A Taha, Wafaa H Hassan, Hamdy H Omar
Co-infection of schistosomiasis, HBV and HCV is common in countries where schistosomiasis is endemic. Occult hepatitis B occurs in patients at high risk for HBV infection (e.g., patients on hemodialysis, patients receiving blood transfusions). Schistosomal infection is a risk factor of HBV infection that can increase the incidence of occult hepatitis B. We aimed to determine the prevalence of occult hepatitis B in chronic hepatitis C patients with and without schistosomiasis and to assess the effect of schistosomal infection on the increased risk of exposure to HBV infection and to occult hepatitis B...
February 9, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28195558/elevated-pre-treatment-il-18-level-is-associated-with-hbeag-seroconversion-in-hiv-hbv-coinfection
#15
Yijia Li, Jing Xie, Huanling Wang, Yang Han, Nidan Wang, Chloe L Thio, Taisheng Li
BACKGROUND: In hepatitis B virus (HBV) infected patients, hepatitis B e antigen (HBeAg) seroconversion is associated with better outcomes. Interleukin-18 (IL-18) controls hepatitis B replication in a mouse model. However, its role in treatment response in HIV/HBV co-infected patients is unknown. METHODS: We enrolled 35 treatment-naïve, HBeAg positive, HIV/HBV co-infected patients. HBV DNA, HIV RNA, CD4 cell count, HBV surface antigen (HBsAg) quantification (qHBsAg), HBeAg quantification (qHBeAg) and IL-18 levels were measured prior to, at 24 and 48 weeks of HBV-active combination antiretroviral therapy (cART)...
February 14, 2017: Antiviral Therapy
https://www.readbyqxmd.com/read/28195337/hepatitis-b-reactivation-in-hepatitis-b-and-c-coinfected-patients-treated-with-antiviral-agents-a-systematic-review-and-meta-analysis
#16
Guofeng Chen, Cheng Wang, Jing Chen, Dong Ji, Yudong Wang, Vanessa Wu, J Karlberg, George Lau
: There is an increased awareness of hepatitis B (HBV) reactivation in chronic hepatitis C (CHC) patients coinfected with HBV treated with pan-oral direct-acting antivirals (DAAs). We performed a systematic review and meta-analysis to compare the rate of HBV reactivation in CHC patients coinfected with overt HBV (hepatitis B surface antigen [HBsAg] positive) and occult HBV (HBsAg negative with positive HBV DNA) infection separately, treated with interferon (IFN)-based therapy to those with pan-oral DAAs...
February 13, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28188612/hepatitis-b-immunoglobulin-during-pregnancy-for-prevention-of-mother-to-child-transmission-of-hepatitis-b-virus
#17
REVIEW
Ahizechukwu C Eke, George U Eleje, Uzoamaka A Eke, Yun Xia, Jiao Liu
BACKGROUND: Hepatitis is a viral infection of the liver. It is mainly transmitted between people through contact with infected blood, frequently from mother to baby in-utero. Hepatitis B poses significant risk to the fetus and up to 85% of infants infected by their mothers at birth develop chronic hepatitis B virus (HBV) infection. Hepatitis B immunoglobulin (HBIG) is a purified solution of human immunoglobulin that could be administered to the mother, newborn, or both. HBIG offers protection against HBV infection when administered to pregnant women who test positive for hepatitis B envelope antigen (HBeAg) or hepatitis B surface antigen (HBsAg), or both...
February 11, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28186991/c-terminal-truncated-hepatitis-b-virus-x-protein-regulates-tumorigenicity-self-renewal-and-drug-resistance-via-stat3-nanog-signaling-pathway
#18
Rachel Hiu Ha Ching, Karen Man Fong Sze, Eunice Yuen Ting Lau, Yung-Tuen Chiu, Joyce Man Fong Lee, Irene Oi Lin Ng, Terence Kin Wah Lee
Hepatitis B virus (HBV) is a major risk factor of chronic liver disease and hepatocellular carcinoma (HCC). Random integration of HBV DNA into the host genome is frequent in HCC leading to truncation of the HBV DNA, particularly at the C-terminal end of the HBV X protein (HBx). C-terminally truncated HBx (HBx-ΔC) has been implicated in playing a pro-oncogenic role in hepatocarcinogenesis. However, the mechanism whereby HBx-ΔC1 contributes to hepatocarcinogenesis remains unclear. In this study, we investigated the functional role of HBx-ΔC1 in regulating liver cancer stem cell (CSC) properties...
February 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28185363/complete-title-relationship-between-hbsag-hbcrag-and-hepatocellular-carcinoma-in-patients-with-undetectable-hbv-dna-under-nucleos-t-ide-therapy
#19
Ka-Shing Cheung, Wai-Kay Seto, Danny Ka-Ho Wong, Ching-Lung Lai, Man-Fung Yuen
We examined the relationship between hepatitis B surface and core-related antigens (HBsAg, HBcrAg) and hepatocellular carcinoma (HCC) development in patients with undetectable serum HBV DNA receiving nucleos(t)ide analogue (NA). Seventy-six HBV carriers with undetectable HBV DNA (<20 IU/mL) who subsequently developed HCC were compared with 152 matched controls. Clinical and laboratory parameters (including novel assays to measure linearized HBsAg [HQ-HBsAg] and HBcrAg) were analyzed. There were no significant differences in HBsAg/ HQ-HBsAg levels between the two groups...
February 10, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28182626/ultrasensitive-detection-of-serum-hepatitis-b-virus-by-coupling-ultrafiltration-dna-extraction-with-real-time-pcr
#20
Bin Wu, Feng Xiao, Peiwen Li, Yan Du, Jinqiong Lin, Kaihua Ming, Bin Chen, Xiuxia Lei, Banglao Xu, Dayu Liu
BACKGROUND: A simple and reliable DNA extraction of hepatitis B virus (HBV) is critical in developing an ultrasensitive detection method for HBV infection. Current commercially available serum Hepatitis B Virus (HBV) DNA extraction methods are time-consuming, expensive and/or require specialized equipment, which hinders wide adoption of clinical laboratories. This study offers a report on an ultrasensitive HBV DNA detection method by coupling serum HBV DNA extraction by ultrafiltration (UF) with real-time PCR (qPCR) detection...
2017: PloS One
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