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https://www.readbyqxmd.com/read/28808417/down-regulation-of-aminopeptidase-n-and-abc-transporter-subfamily-g-transcripts-in-cry1ab-and-cry1ac-resistant-asian-corn-borer-ostrinia-furnacalis-lepidoptera-crambidae
#1
Tiantao Zhang, Brad S Coates, Yueqin Wang, Yidong Wang, Shuxiong Bai, Zhenying Wang, Kanglai He
The Asian corn borer (ACB), Ostrinia furnacalis (Lepidoptera: Crambidae), is a highly destructive pest of cultivated maize throughout East Asia. Bacillus thuringiensis (Bt) crystalline protein (Cry) toxins cause mortality by a mechanism involving pore formation or signal transduction following toxin binding to receptors along the midgut lumen of susceptible insects, but this mechanism and mutations therein that lead to resistance are not fully understood. In the current study, quantitative comparisons were made among midgut expressed transcripts from O...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28754962/two-dopamine-d2-like-receptor-genes-from-the-silkworm-bombyx-mori-and-their-evolutionary-history-in-metazoan
#2
Ping Chen, Peng Chen, Tian Li, Qi Shen, Deng-Feng Yan, Liang Zhang, Xi Chen, Yan Li, Wei Zhao
Dopamine is widely distributed in metazoans and is implicated in many physiological functions. Dopaminergic signaling is mediated through two classes of dopamine receptors, D1-like and D2-like. Phylogeny analysis reveals that, the dopamine receptors probably appeared ahead of the cnidarian divergence, two distinct classes of dopamine receptors likely formed prior to the separation of deuterostomes and protostomes, and INDRs probably split from its ancestor before the emergence of nematodes. Two D2-like genes are closely linked on the same scaffold, and the chromosome region around D2-like gene loci show colinearity among different species within Lepidoptera...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28680812/new-reporter-gene-assays-for-detecting-natural-and-synthetic-molting-hormone-agonists-using-yeasts-expressing-ecdysone-receptors-of-various-insects
#3
Sayoko Ito-Harashima, Mai Matsuura, Masanobu Kawanishi, Yoshiaki Nakagawa, Takashi Yagi
Synthetic nonsteroidal ecdysone agonists, a class of insect growth regulators (IGRs), target the ecdysone receptor (EcR), which forms a heterodimer with ultraspiracle (USP) to transactivate ecdysone response genes. These compounds have high binding affinities to the EcR-USP complexes of certain insects and their toxicity is selective for certain taxonomic orders. In the present study, we developed reporter gene assay (RGA) systems to detect molting hormone (ecdysone) activity by introducing EcR-USP cDNA and a bacterial lacZ reporter gene into yeast...
July 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28663125/characterization-of-three-serotonin-receptors-from-the-small-white-butterfly-pieris-rapae
#4
Yi-Xiang Qi, Miao Jin, Xu-Yang Ni, Gong-Yin Ye, Youngseok Lee, Jia Huang
Serotonin (5-hydroxytryptamine, 5-HT) plays a key role in modulating diverse physiological processes and behaviors in both protostomes and deuterostomes. These functions are mediated through the binding of serotonin to its receptors, which are recognized as potential insecticide targets. We investigated the sequence, pharmacology and tissue distribution of three 5-HT receptors (Piera5-HT1A, Piera5-HT1B, Piera5-HT7) from the small white butterfly Pieris rapae, an important pest of cultivated cabbages and other mustard family crops...
August 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28618068/molecular-cloning-and-analysis-of-a-c-type-lectin-from-silkworm-bombyx-mori
#5
Toufeeq Shahzad, Ming-Yue Zhan, Pei-Jin Yang, Xiao-Qiang Yu, Xiang-Jun Rao
C-type lectins (CTLs) play a variety of roles in plants and animals. They are involved in animal development, pathogen recognition, and the activation of immune responses. CTLs carry one or more non-catalytic carbohydrate-recognition domains (CRDs) to bind specific carbohydrates reversibly. Here, we report the molecular cloning and functional analysis of a single-CRD CTL, named C-type lectin-S2 (BmCTL-S2) from the domesticated silkmoth Bombyx mori (Lepidoptera: Bombycidae). The ORF of CTL-S2 is 666 bp, which encodes a putative protein of 221 amino acids...
June 15, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28614384/antennal-transcriptome-analysis-of-the-piercing-moth-oraesia-emarginata-lepidoptera-noctuidae
#6
Bo Feng, Qianshuang Guo, Kaidi Zheng, Yuanxia Qin, Yongjun Du
The piercing fruit moth Oraesia emarginata is an economically significant pest; however, our understanding of its olfactory mechanisms in infestation is limited. The present study conducted antennal transcriptome analysis of olfactory genes using real-time quantitative reverse transcription PCR analysis (RT-qPCR). We identified a total of 104 candidate chemosensory genes from several gene families, including 35 olfactory receptors (ORs), 41 odorant-binding proteins, 20 chemosensory proteins, 6 ionotropic receptors, and 2 sensory neuron membrane proteins...
2017: PloS One
https://www.readbyqxmd.com/read/28580965/functional-evolution-of-lepidoptera-olfactory-receptors-revealed-by-deorphanization-of-a-moth-repertoire
#7
Arthur de Fouchier, William B Walker, Nicolas Montagné, Claudia Steiner, Muhammad Binyameen, Fredrik Schlyter, Thomas Chertemps, Annick Maria, Marie-Christine François, Christelle Monsempes, Peter Anderson, Bill S Hansson, Mattias C Larsson, Emmanuelle Jacquin-Joly
Insects detect their hosts or mates primarily through olfaction, and olfactory receptors (ORs) are at the core of odorant detection. Each species has evolved a unique repertoire of ORs whose functional properties are expected to meet its ecological needs, though little is known about the molecular basis of olfaction outside Diptera. Here we report a pioneer functional analysis of a large array of ORs in a lepidopteran, the herbivorous pest Spodoptera littoralis. We demonstrate that most ORs are narrowly tuned to ubiquitous plant volatiles at low, relevant odorant titres...
June 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/28474181/sexual-selection-on-receptor-organ-traits-younger-females-attract-males-with-longer-antennae
#8
Tamara L Johnson, Matthew R E Symonds, Mark A Elgar
Sexual selection theory predicts that female choice may favour the evolution of elaborate male signals. Darwin also suggested that sexual selection can favour elaborate receiver structures in order to better detect sexual signals, an idea that has been largely ignored. We evaluated this unorthodox perspective by documenting the antennal lengths of male Uraba lugens Walker (Lepidoptera: Nolidae) moths that were attracted to experimentally manipulated emissions of female sex pheromone. Either one or two females were placed in field traps for the duration of their adult lives in order to create differences in the quantity of pheromone emissions from the traps...
June 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/28387246/sensory-genes-identification-with-head-transcriptome-of-the-migratory-armyworm-mythimna-separata
#9
Zhenxing Liu, Xiaoyun Wang, Chaoliang Lei, Fen Zhu
Sensory system plays important roles in a wide array of insect's behavior and physiological events, including the host landing and locating, feeding, flying, sex responding, mating and oviposition which happen independently and in sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) of migratory insect is destructive for alimentarn crop and economic crop throughout the world. Here we present the high throughput sequencing of the head transcriptome and identify members of the major sensory genes which are crucial for armyworm's success worldwide, including 8 opsins, 22 chemosensory proteins, 50 odorant binding proteins, 60 odorant receptors, 8 gustatory receptors, 24 ionotropic receptors, and 2 sensory neuron membrane proteins...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28331183/differential-expression-of-chemosensory-protein-genes-in-midguts-in-response-to-diet-of-spodoptera-litura
#10
Xin Yi, Jiangwei Qi, Xiaofan Zhou, Mei Ying Hu, Guo Hua Zhong
While it has been well characterized that chemosensory receptors in guts of mammals have great influence on food preference, much remains elusive in insects. Insect chemosensory proteins (CSPs) are soluble proteins that could deliver chemicals to olfactory and gustatory receptors. Recent studies have identified a number of CSPs expressed in midgut in Lepidoptera insects, which started to reveal their roles in chemical recognition and stimulating appetite in midgut. In this study, we examined expression patterns in midgut of 21 Spodoptera litura CSPs (SlitCSPs) characterized from a previously reported transcriptome, and three CSPs were identified to be expressed highly in midgut...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28266104/larvicidal-activity-of-synthetic-tropane-alkaloids-against-ascia-monuste-orseis-lepidoptera-pieridae
#11
Simone Z Mairink, Luiz Ca Barbosa, Eduardo Vv Varejão, Elizeu S Farias, Márcio Lm Santos, Marcelo C Picanço
BACKGROUND: Tropane alkaloids are well known to play a role in plant defense. By blocking acetylcholine receptors, they exert insecticidal and deterrent effects against herbivore insects. Carbamates are an important class of chemical insecticides that also inhibit acetyl cholinesterase. The objective of this work was to synthesize a series of tropane alkaloids bearing a carbamate group, and to evaluate their effects against the pest Ascia monuste. The effects of the most active compounds were evaluated on the A...
March 6, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28223048/vegetative-insecticidal-protein-of-bacillus-thuringiensis-blb459-and-its-efficiency-against-lepidoptera
#12
Hanen Boukedi, Saoussen Ben Khedher, Rania Hadhri, Samir Jaoua, Slim Tounsi, Lobna Abdelkefi-Mesrati
Bacillus thuringiensis strain BLB459 supernatant showed a promising activity against Lepidopteran pests with extremely damages in the larvae midgut. Investigations of the genes that encode secreted toxin demonstrated that this strain harbored a vip3-type gene named vip3(459). Based on its original nucleotide and amino acid sequences, this gene was cloned into pET-14b vector and overexpressed in Escherichia coli. The expressed protein was purified and tested against different insects and interestingly the novel toxin demonstrated a remarkable activity against the stored products pest Ephestia kuehniella and the polyphagous insects Spodoptera littoralis and Agrotis segetum...
April 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28186125/responses-of-the-cutworm-spodoptera-litura-lepidoptera-noctuidae-to-two-bt-corn-hybrids-expressing-cry1ab
#13
Shu Yinghua, Du Yan, Chen Jin, Wei Jiaxi, Wang Jianwu
To examine the responses of the secondary lepidopteran pest Spodoptera litura to two Bacillus thuringiensis (Bt) corn hybrids [5422Bt1 (Event Bt11), 5422CBCL (MON810)] expressing Cry1Ab, larval bioassays with Cry1Ab toxin, corn leaves or kernels and bagging on corn plants were conducted. The results showed that larvae displayed a similar performance when fed kernels, but not leaves of 5422Bt1, 5422CBCL and their near-isogenic non-Bt corn (5422). Significantly higher Cry1Ab amounts were detected in larvae fed leaves than kernels of both Bt hybrids, with different molecular weights of protein band in plants (72 and 90 kDa for 5422Bt1 and 5422CBCL, respectively), gut contents (65 kDa), feces (50 kDa), which indicated that larvae had lower ingestion, higher degradation and excretion of Cry1Ab when fed kernels not leaves of both Bt hybrids...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28185941/expression-map-of-a-complete-set-of-gustatory-receptor-genes-in-chemosensory-organs-of-bombyx-mori
#14
Huizhen Guo, Tingcai Cheng, Zhiwei Chen, Liang Jiang, Youbing Guo, Jianqiu Liu, Shenglong Li, Kiyoko Taniai, Kiyoshi Asaoka, Keiko Kadono-Okuda, Kallare P Arunkumar, Jiaqi Wu, Hirohisa Kishino, Huijie Zhang, Rakesh K Seth, Karumathil P Gopinathan, Nicolas Montagné, Emmanuelle Jacquin-Joly, Marian R Goldsmith, Qingyou Xia, Kazuei Mita
Most lepidopteran species are herbivores, and interaction with host plants affects their gene expression and behavior as well as their genome evolution. Gustatory receptors (Grs) are expected to mediate host plant selection, feeding, oviposition and courtship behavior. However, due to their high diversity, sequence divergence and extremely low level of expression it has been difficult to identify precisely a complete set of Grs in Lepidoptera. By manual annotation and BAC sequencing, we improved annotation of 43 gene sequences compared with previously reported Grs in the most studied lepidopteran model, the silkworm, Bombyx mori, and identified 7 new tandem copies of BmGr30 on chromosome 7, bringing the total number of BmGrs to 76...
March 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28087271/mesoionic-pyrido-1-2-a-pyrimidinones-discovery-of-dicloromezotiaz-as-a-lepidoptera-insecticide-acting-on-nicotinic-acetylcholine-receptors-1-2
#15
Wenming Zhang, Caleb W Holyoke, James Barry, Daniel Cordova, Robert M Leighty, My-Hanh T Tong, Kenneth A Hughes, George P Lahm, Thomas F Pahutski, Ming Xu, Twyla A Briddell, Stephen F McCann, Yewande T Henry, Yuzhong Chen
A novel class of mesoionic pyrido[1,2-a]pyrimidinones has been discovered with exceptional insecticidal activity controlling a number of insect species. In this communication, we report the part of the optimization program that led to the identification of dicloromezotiaz as a potent insecticide to control a broad range of lepidoptera. Our efforts in discovery, synthesis, structure-activity relationship elucidation, and biological activity evaluation are also presented.
February 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28043334/ryanodine-receptor-genes-of-the-rice-stem-borer-chilo-suppressalis-molecular-cloning-alternative-splicing-and-expression-profiling
#16
Y C Peng, C W Sheng, John E Casida, C Q Zhao, Z J Han
The ryanodine receptor (RyR) of the calcium release channel is the main target of anthranilic and phthalic diamide insecticides which have high selective insecticidal activity relative to mammalian toxicity. In this study, the full-length cDNA of Chilo suppressalis RyR (CsRyR) was isolated and characterized. The CsRyR mRNA has an open reading frame (ORF) of 15,387bp nucleotides, which encodes 5128 amino acids with GenBank ID: KR088972. Comparison of protein sequences showed that CsRyR shared high identities with other insects of 77-96% and lower identity to mammals and nematodes with only 42-45%...
January 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/27986933/the-spodoptera-exigua-lepidoptera-noctuidae-abcc2-mediates-cry1ac-cytotoxicity-and-in-conjunction-with-cadherin-contributes-to-enhance-cry1ca-toxicity-in-sf9-cells
#17
Xiang-Liang Ren, Wei-Li Jiang, Ya-Jie Ma, Hong-Yan Hu, Xiao-Yan Ma, Yan Ma, Guo-Qing Li
In insects, the mode of Cry1A toxins action has been studied in detail and many receptors that participate in the process are known. Recent evidence has revealed that an ABC transporter (ABCC2) is involved in conferring resistance to Cry1A toxins and that ABCC2 could be a receptor of Cry1A. However, it is not known whether Cry1Ca interacts with the same receptor proteins as Cry1A. In this study, we report the cloning of an ABC transporter gene, SeABCC2b, from the midgut of Spodoptera exigua (Hübner) larvae, and its expression in Sf9 cells for a functional analysis...
December 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27958372/global-map-of-oxytocin-vasopressin-like-neuropeptide-signalling-in-insects
#18
Zita Liutkeviciute, Johannes Koehbach, Thomas Eder, Esther Gil-Mansilla, Christian W Gruber
Oxytocin and vasopressin mediate a range of physiological functions that are important for osmoregulation, reproduction, social behaviour, memory and learning. The origin of this signalling system is thought to date back ~600 million years. Oxytocin/vasopressin-like peptides have been identified in several invertebrate species and they appear to be functionally related across the entire animal kingdom. There is little information available about the biology of this peptide G protein-coupled receptor signalling system in insects...
December 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27929397/a-p-glycoprotein-is-linked-to-resistance-to-the-bacillus-thuringiensis-cry3aa-toxin-in-a-leaf-beetle
#19
Yannick Pauchet, Anne Bretschneider, Sylvie Augustin, David G Heckel
Chrysomela tremula is a polyvoltine oligophagous leaf beetle responsible for massive attacks on poplar trees. This beetle is an important model for understanding mechanisms of resistance to Bacillus thuringiensis (Bt) insecticidal toxins, because a resistant C. tremula strain has been found that can survive and reproduce on transgenic poplar trees expressing high levels of the Cry3Aa Bt toxin. Resistance to Cry3Aa in this strain is recessive and is controlled by a single autosomal locus. We used a larval midgut transcriptome for C...
December 5, 2016: Toxins
https://www.readbyqxmd.com/read/27914919/expressing-a-moth-abcc2-gene-in-transgenic-drosophila-causes-susceptibility-to-bt-cry1ac-without-requiring-a-cadherin-like-protein-receptor
#20
Tristan Stevens, Sisi Song, John B Bruning, Amanda Choo, Simon W Baxter
Bt toxins ingested by insect pests can bind to midgut receptors and cause death, although several steps in this process remain unclear. Multiple Bt toxin receptors have been identified in Lepidoptera, including a cadherin-like protein (CaLP), which is central to several models explaining Bt toxins' mode of action. Mutations in the Plutella xylostella ATP-dependent binding cassette transporter C2 (Px-abcc2), rather than CaLP, are genetically linked with Bt Cry1Ac resistance. Here we expressed Px-abcc2 in Drosophila and performed larval bioassays to determine whether this protein acts as an effective Bt receptor...
January 2017: Insect Biochemistry and Molecular Biology
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