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https://www.readbyqxmd.com/read/28223048/vegetative-insecticidal-protein-of-bacillus-thuringiensis-blb459-and-its-efficiency-against-lepidoptera
#1
Hanen Boukedi, Saoussen Ben Khedher, Rania Hadhri, Samir Jaoua, Slim Tounsi, Lobna Abdelkefi-Mesrati
Bacillus thuringiensis strain BLB459 supernatant showed a promising activity against Lepidopteran pests with extremely damages in the larvae midgut. Investigations of the genes that encode secreted toxin demonstrated that this strain harbored a vip3-type gene named vip3(459). Based on its original nucleotide and amino acid sequences, this gene was cloned into pET-14b vector and overexpressed in Escherichia coli. The expressed protein was purified and tested against different insects and interestingly the novel toxin demonstrated a remarkable activity against the stored products pest Ephestia kuehniella and the polyphagous insects Spodoptera littoralis and Agrotis segetum...
February 18, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28185941/expression-map-of-a-complete-set-of-gustatory-receptor-genes-in-chemosensory-organs-of-bombyx-mori
#2
Huizhen Guo, Tingcai Cheng, Zhiwei Chen, Liang Jiang, Youbing Guo, Jianqiu Liu, Shenglong Li, Kiyoko Taniai, Kiyoshi Asaoka, Keiko Kadono-Okuda, Kallare P Arunkumar, Jiaqi Wu, Hirohisa Kishino, Huijie Zhang, Rakesh K Seth, Karumathil P Gopinathan, Nicolas Montagné, Emmanuelle Jacquin-Joly, Marian R Goldsmith, Qingyou Xia, Kazuei Mita
Most lepidopteran species are herbivores, and interaction with host plants affects their gene expression and behavior as well as their genome evolution. Gustatory receptors (Grs) are expected to mediate host plant selection, feeding, oviposition and courtship behavior. However, due to their high diversity, sequence divergence and extremely low level of expression it has been difficult to identify precisely a complete set of Grs in Lepidoptera. By manual annotation and BAC sequencing, we improved annotation of 43 gene sequences compared with previously reported Grs in the most studied lepidopteran model, the silkworm, Bombyx mori, and identified 7 new tandem copies of BmGr30 on chromosome 7, bringing the total number of BmGrs to 76...
February 6, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28067269/proteomic-analysis-of-cry2aa-binding-proteins-and-their-receptor-function-in-spodoptera-exigua
#3
Lin Qiu, Boyao Zhang, Lang Liu, Weihua Ma, Xiaoping Wang, Chaoliang Lei, Lizhen Chen
The bacterium Bacillus thuringiensis produces Crystal (Cry) proteins that are toxic to a diverse range of insects. Transgenic crops that produce Bt Cry proteins are grown worldwide because of their improved resistance to insect pests. Although Bt "pyramid" cotton that produces both Cry1A and Cry2A is predicted to be more resistant to several lepidopteran pests, including Spodoptera exigua, than plants that produce Cry1Ac alone, the mechanisms responsible for the toxicity of Cry2Aa in S. exigua are not well understood...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28059141/the-molecular-sensory-machinery-of-a-chagas-disease-vector-expression-changes-through-imaginal-moult-and-sexually-dimorphic-features
#4
Jose Manuel Latorre-Estivalis, Hugh M Robertson, Kimberly K O Walden, Jerônimo Ruiz, Leilane Oliveira Gonçalves, Alessandra A Guarneri, Marcelo Gustavo Lorenzo
The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5(th) instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043334/ryanodine-receptor-genes-of-the-rice-stem-borer-chilo-suppressalis-molecular-cloning-alternative-splicing-and-expression-profiling
#5
Y C Peng, C W Sheng, John E Casida, C Q Zhao, Z J Han
The ryanodine receptor (RyR) of the calcium release channel is the main target of anthranilic and phthalic diamide insecticides which have high selective insecticidal activity relative to mammalian toxicity. In this study, the full-length cDNA of Chilo suppressalis RyR (CsRyR) was isolated and characterized. The CsRyR mRNA has an open reading frame (ORF) of 15,387bp nucleotides, which encodes 5128 amino acids with GenBank ID: KR088972. Comparison of protein sequences showed that CsRyR shared high identities with other insects of 77-96% and lower identity to mammals and nematodes with only 42-45%...
January 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/27992549/molecular-characterization-and-functional-analysis-of-a-putative-octopamine-tyramine-receptor-during-the-developmental-stages-of-the-pacific-oyster-crassostrea-gigas
#6
Peng Ji, Fei Xu, Baoyu Huang, Yingxiang Li, Li Li, Guofan Zhang
Octopamine (OA) and its precursor, tyramine (TA), participate in invertebrate development such as growth, maturation, and reproduction by activating their corresponding G protein-coupled receptors (GPCRs). Although OA was first discovered in mollusks (octopus), subsequent studies on OA, TA and related receptors have primarily been conducted in Ecdysozoa, especially in insects. Accordingly, only limited reports on OA/TA receptors in mollusks are available and their physiological roles remain unclear. Here, a full-length cDNA encoding a putative 524 amino acid OA/TA receptor (CgGPR1) was isolated from the Pacific oyster Crassostrea gigas...
2016: PloS One
https://www.readbyqxmd.com/read/27986933/the-spodoptera-exigua-lepidoptera-noctuidae-abcc2-mediates-cry1ac-cytotoxicity-and-in-conjunction-with-cadherin-contributes-to-enhance-cry1ca-toxicity-in-sf9-cells
#7
Xiang-Liang Ren, Wei-Li Jiang, Ya-Jie Ma, Hong-Yan Hu, Xiao-Yan Ma, Yan Ma, Guo-Qing Li
In insects, the mode of Cry1A toxins action has been studied in detail and many receptors that participate in the process are known. Recent evidence has revealed that an ABC transporter (ABCC2) is involved in conferring resistance to Cry1A toxins and that ABCC2 could be a receptor of Cry1A. However, it is not known whether Cry1Ca interacts with the same receptor proteins as Cry1A. In this study, we report the cloning of an ABC transporter gene, SeABCC2b, from the midgut of Spodoptera exigua (Hübner) larvae, and its expression in Sf9 cells for a functional analysis...
December 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27982028/a-molecular-and-neuronal-basis-for-amino-acid-sensing-in-the-drosophila-larva
#8
Vincent Croset, Michael Schleyer, J Roman Arguello, Bertram Gerber, Richard Benton
Amino acids are important nutrients for animals, reflected in conserved internal pathways in vertebrates and invertebrates for monitoring cellular levels of these compounds. In mammals, sensory cells and metabotropic glutamate receptor-related taste receptors that detect environmental sources of amino acids in food are also well-characterised. By contrast, it is unclear how insects perceive this class of molecules through peripheral chemosensory mechanisms. Here we investigate amino acid sensing in Drosophila melanogaster larvae, which feed ravenously to support their rapid growth...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27918112/validation-of-adam10-metalloprotease-as-a-bacillus-thuringiensis-cry3aa-toxin-functional-receptor-in-colorado-potato-beetle-leptinotarsa-decemlineata
#9
V M Ruiz-Arroyo, I García-Robles, C Ochoa-Campuzano, G A Goig, E Zaitseva, G Baaken, A C Martínez-Ramírez, C Rausell, M D Real
Bacillus thuringiensis parasporal crystal proteins (Cry proteins) are insecticidal pore-forming toxins that bind to specific receptor molecules on the brush border membrane of susceptible insect midgut cells to exert their toxic action. In the Colorado potato beetle (CPB), a coleopteran pest, we previously proposed that interaction of Cry3Aa toxin with a CPB ADAM10 metalloprotease is an essential part of the mode of action of this toxin. Here, we annotated the gene sequence encoding an ADAM10 metalloprotease protein (CPB-ADAM10) in the CPB genome sequencing project, and using RNA interference gene silencing we demonstrated that CPB-ADAM10 is a Cry3Aa toxin functional receptor in CPB...
December 5, 2016: Insect Molecular Biology
https://www.readbyqxmd.com/read/27914919/expressing-a-moth-abcc2-gene-in-transgenic-drosophila-causes-susceptibility-to-bt-cry1ac-without-requiring-a-cadherin-like-protein-receptor
#10
Tristan Stevens, Sisi Song, John B Bruning, Amanda Choo, Simon W Baxter
Bt toxins ingested by insect pests can bind to midgut receptors and cause death, although several steps in this process remain unclear. Multiple Bt toxin receptors have been identified in Lepidoptera, including a cadherin-like protein (CaLP), which is central to several models explaining Bt toxins' mode of action. Mutations in the Plutella xylostella ATP-dependent binding cassette transporter C2 (Px-abcc2), rather than CaLP, are genetically linked with Bt Cry1Ac resistance. Here we expressed Px-abcc2 in Drosophila and performed larval bioassays to determine whether this protein acts as an effective Bt receptor...
January 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27908826/itraq-based-quantitative-proteomic-analysis-of-midgut-in-silkworm-infected-with-bombyx-mori-cytoplasmic-polyhedrosis-virus
#11
Kun Gao, Xiang-Yuan Deng, Meng-Ke Shang, Guang-Xing Qin, Cheng-Xiang Hou, Xi-Jie Guo
: Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) specifically infects the epithelial cells in the midgut of silkworm and causes them to death, which negatively affects the sericulture industry. In order to determine the midgut response at the protein levels to the virus infection, differential proteomes of the silkworm midgut responsive to BmCPV infection were identified with isobaric tags for relative and absolute quantitation (iTRAQ) labeling followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27883266/silencing-of-ecdysone-receptor-insect-intestinal-mucin-and-sericotropin-genes-by-bacterially-produced-double-stranded-rna-affects-larval-growth-and-development-in-plutella-xylostella-and-helicoverpa-armigera
#12
B Israni, M V Rajam
RNA interference mediated gene silencing, which is triggered by double-stranded RNA (dsRNA), has become a important tool for functional genomics studies in various systems, including insects. Bacterially produced dsRNA employs the use of a bacterial strain lacking in RNaseIII activity and harbouring a vector with dual T7 promoter sites, which allow the production of intact dsRNA molecules. Here, we report an assessment of the functional relevance of the ecdysone receptor, insect intestinal mucin and sericotropin genes through silencing by dsRNA in two lepidopteran insect pests, Helicoverpa armigera and Plutella xylostella, both of which cause serious crop losses...
November 24, 2016: Insect Molecular Biology
https://www.readbyqxmd.com/read/27867074/toxicity-of-cry1a-toxins-from-bacillus-thuringiensis-to-cf1-cells-does-not-involve-activation-of-adenylate-cyclase-pka-signaling-pathway
#13
Leivi Portugal, Carlos Muñóz-Garay, Diana L Martínez de Castro, Mario Soberón, Alejandra Bravo
Bacillus thuringiensis (Bt) bacteria produce Cry toxins that are able to kill insect pests. Different models explaining the mode of action of these toxins have been proposed. The pore formation model proposes that the toxin creates pores in the membrane of the larval midgut cells after interaction with different receptors such as cadherin, aminopeptidase N and alkaline phosphatase and that this pore formation activity is responsible for the toxicity of these proteins. The alternative model proposes that interaction with cadherin receptor triggers an intracellular cascade response involving protein G, adenylate cyclase (AC) and protein kinase A (PKA)...
January 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27862222/exposure-to-chlorantraniliprole-affects-the-energy-metabolism-of-the-caddisfly-sericostoma-vittatum
#14
Andreia C M Rodrigues, Carlos Gravato, Carla Quintaneiro, Maria D Bordalo, Oksana Golovko, Vladimír Žlábek, Carlos Barata, Amadeu M V M Soares, João L T Pestana
Caddisflies have been included in ecotoxicological studies because of their sensitivity and ecological relevance. The present study aimed to assess the sublethal effects of an anthranilic diamide insecticide, chlorantraniliprole (CAP), to Sericostoma vittatum. Used worldwide, CAP is a persistent compound that has been found in surface waters at concentrations from 0.1 μg/L to 9.7 μg/L. It targets the ryanodine receptors, and the present ecotoxicological assessment focused on biomarkers related to neurotransmission, biotransformation, oxidative stress damage, and endpoints related to energy processing (feeding, energy reserves, and cellular metabolism)...
November 10, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27823898/rnai-in-the-striped-stem-borer-chilo-suppressalis-establishes-a-functional-role-for-aminopeptidase-n-in-cry1ab-intoxication
#15
X Y Wang, L X Du, C X Liu, L Gong, L Z Han, Y F Peng
The striped stem borer, Chilo suppressalis, is a major target pest of transgenic rice expressing the Cry1Ab protein from the bacterium Bacillus thuringiensis (Bt) in China. Evolution of resistance in this pest is a major threat to the durability of Bt rice. Since Bt exerts its activity through binding to specific receptors in the midgut of target insects, identification of functional Cry1Ab receptors in the midgut of C. suppressalis larvae is crucial to evaluate potential resistance mechanisms and develop effective strategies for delaying insect resistance...
November 4, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27779241/comparative-venomics-of-psyttalia-lounsburyi-and-p-concolor-two-olive-fruit-fly-parasitoids-a-hypothetical-role-for-a-gh1-%C3%AE-glucosidase
#16
Hugo Mathé-Hubert, Dominique Colinet, Emeline Deleury, Maya Belghazi, Marc Ravallec, Julie Poulain, Carole Dossat, Marylène Poirié, Jean-Luc Gatti
Venom composition of parasitoid wasps attracts increasing interest - notably molecules ensuring parasitism success on arthropod pests - but its variation within and among taxa is not yet understood. We have identified here the main venom proteins of two braconid wasps, Psyttalia lounsburyi (two strains from South Africa and Kenya) and P. concolor, olive fruit fly parasitoids that differ in host range. Among the shared abundant proteins, we found a GH1 β-glucosidase and a family of leucine-rich repeat (LRR) proteins...
October 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27713019/galleria-mellonella-hemocytes-a-novel-phagocytic-assay-for-leishmania-viannia-braziliensis
#17
Fernanda Tomiotto-Pellissier, Allan Henrique Depieri Cataneo, Tatiane Marcuso Orsini, Ana Paula Fortes Dos Santos Thomazelli, Gabriela Alcântara Dalevedo, Admilton Gonçalves de Oliveira, Luciano Aparecido Panagio, Idessania Nazareth Costa, Ivete Conchon-Costa, Wander Rogério Pavanelli, Ricardo Sergio Almeida
Galleria mellonella is an excellent invertebrate model for the study of diseases that involve interactions with cells from the innate immune system, since they have an innate immune system capable of recognizing the pathogens. Here we present for the first time, an alternative model for an in vitro phagocytic assay using hemocytes of G. mellonella larvae to study infection by Leishmania (Viannia) braziliensis. We showed that the insect phagocytic cells were able to engulf promastigotes. Furthermore, this infective form differentiated into the amastigote form inside those cells...
October 3, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27706708/characterization-of-a-novel-cry8ea3-binding-v-atpase-subunit-a-in-holotrichia-parallela
#18
W Wei, G Wei, Z Dan, Y Xiaoping, Z Yakun
Several receptor proteins of Cry toxin have been previously identified, including cadherin-like, aminopeptidase N, and alkaline phosphatase. In the present work, a novel binding protein, V-ATPase subunit A (HpVAA), was identified in Holotricia parallela larvae and characterized. We performed reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends technology to obtain the cDNA of the full-length hpvaa. Sequencing analysis showed that the open reading frame of hpvaa (GenBank accession No...
September 2, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27658924/toward-an-understanding-of-divergent-compound-eye-development-in-drones-and-workers-of-the-honeybee-apis-mellifera-l-a-correlative-analysis-of-morphology-and-gene-expression
#19
David S Marco Antonio, Klaus Hartfelder
Eye development in insects is best understood in Drosophila melanogaster, but little is known for other holometabolous insects. Combining a morphological with a gene expression analysis, we investigated eye development in the honeybee, putting emphasis on the sex-specific differences in eye size. Optic lobe development starts from an optic lobe anlage in the larval brain, which sequentially gives rise to the lobula, medulla, and lamina. The lamina differentiates in the last larval instar, when it receives optic nerve projections from the developing retina...
January 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/27618036/susceptibility-and-immune-defence-mechanisms-of-rhynchophorus-ferrugineus-olivier-coleoptera-curculionidae-against-entomopathogenic-fungal-infections
#20
Abid Hussain, Muhammad Rizwan-Ul-Haq, Hassan Al-Ayedh, Ahmed Mohammed AlJabr
Insects infected with entomopathogenic fungi, experience physiological changes that influence their growth and immune defence. The potential of nine isolates of entomopathogenic fungi was evaluated after determining percent germination and relative conidial hydrophobicity. However, nutritional indices were evaluated after immersing eighth-instar Rhynchophorus ferrugineus larvae into each isolate suspension (1 × 10⁷ conidia/mL). The results showed that isolates B6884 and M9374 had 44.51% and 39.02% higher conidial hydrophobicity compared with isolate I03011 (least virulent)...
September 9, 2016: International Journal of Molecular Sciences
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