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https://www.readbyqxmd.com/read/28427951/uncovering-the-immune-responses-of-apis-mellifera-ligustica-larval-gut-to-ascosphaera-apis-infection-utilizing-transcriptome-sequencing
#1
Dafu Chen, Rui Guo, Xijian Xu, Cuiling Xiong, Qin Liang, Yanzhen Zheng, Qun Luo, Zhaonan Zhang, Zhijian Huang, Dhiraj Kumar, Weijun Xi, Xuan Zou, Min Liu
Honeybees are susceptible to a variety of diseases, including chalkbrood, which is capable of causing huge losses of both the number of bees and colony productivity. This research is designed to characterize the transcriptome profiles of Ascosphaera apis-treated and un-treated larval guts of Apis mellifera ligustica in an attempt to unravel the molecular mechanism underlying the immune responses of western honeybee larval guts to mycosis. In this study, 24, 296 and 2157 genes were observed to be differentially expressed in A...
April 18, 2017: Gene
https://www.readbyqxmd.com/read/28389549/identification-of-holotrichia-oblita-midgut-proteins-that-bind-to-cry8-like-toxin-from-bacillus-thuringiensis-and-assembling-of-h-oblita-midgut-tissue-transcriptome
#2
Jian Jiang, Ying Huang, Changlong Shu, Mario Soberón, Alejandra Bravo, Chunqing Liu, Fuping Song, Jinsheng Lai, Jie Zhang
The Bacillus thuringiensis strain HBF-18 (CGMCC2070), containing two cry genes (cry8-like and cry8Ga), is toxic to Holotrichia oblita larvae. Both Cry8-like and Cry8Ga proteins are active against this insect pest, while Cry8-like is the more toxic protein. To analyze the binding characteristics of Cry8-like and Cry8Ga proteins to brush border membrane vesicles (BBMV) in H. oblita larvae, binding assays were conducted with a fluorescent DyLight488-labeled Cry8-like toxin. Results of binding saturation assays demonstrated that Cry8-like binds specifically to binding sites on BBMV from H...
April 7, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28369111/larval-diapause-termination-in-the-bamboo-borer-omphisa-fuscidentalis
#3
Suphawan Suang, Manaporn Manaboon, Tippawan Singtripop, Kiyoshi Hiruma, Yu Kaneko, Pimonrat Tiansawat, Peter Neumann, Panuwan Chantawannakul
In insects, juvenile hormone (JH) and 20-hydroxyecdysone (20E) regulate larval growth and molting. However, little is known about how this cooperative control is terminating larval diapause especially in the bamboo borer, Omphisa fuscidentalis. In both in vivo and in vitro experiments, we here measured the expression levels of genes which were affected by juvenile hormone analogue (JHA: S-methoprene) and 20-hydroxyecdysone (20E) in diapausing O. fuscidentalis larvae. Corresponding mRNA expression changes in the subesophageal ganglion (SG) and prothoracic gland (PG) were evaluated using qRT-PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28358336/patterns-of-gene-expression-in-western-corn-rootworm-diabrotica-virgifera-virgifera-neonates-challenged-with-cry34ab1-cry35ab1-and-cry34-35ab1-based-on-next-generation-sequencing
#4
Haichuan Wang, Seong-Il Eyun, Kanika Arora, Sek Yee Tan, Premchand Gandra, Etsuko Moriyama, Chitvan Khajuria, Jessica Jurzenski, Huarong Li, Maia Donahue, Ken Narva, Blair Siegfried
With Next Generation Sequencing technologies, high-throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control...
March 30, 2017: Toxins
https://www.readbyqxmd.com/read/28274717/rna-interference-knockdown-of-aminopeptidase-n-genes-decrease-the-susceptibility-of-chilo-suppressalis-larvae-to-cry1ab-cry1ac-and-cry1ca-expressing-transgenic-rice
#5
Lin Qiu, Jinxing Fan, Boyao Zhang, Lang Liu, Xiaoping Wang, Chaoliang Lei, Yongjun Lin, Weihua Ma
Transgenic rice expressing Bacillus thuringiensis (Bt) Cry toxins are resistant to lepidopteran pests, such as Chilo suppressalis, a major insect pest of rice in Asia. Understanding how these toxins interact with their hosts is crucial to understanding their insecticidal action. In this study, knockdown of two aminopeptidase N genes (APN1 and APN2) by RNA interference resulted in decreased susceptibility of C. suppressalis larvae to the Bt rice varieties TT51 (Cry1Ab and Cry1Ac fusion genes) and T1C-19 (Cry1Ca), but not T2A-1 (Cry2Aa)...
March 6, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28266104/larvicidal-activity-of-synthetic-tropane-alkaloids-against-ascia-monuste-orseis-lepidoptera-pieridae
#6
Simone Z Mairink, Luiz Ca Barbosa, Eduardo Vv Varejão, Elizeu S Farias, Márcio Lm Santos, Marcelo C Picanço
BACKGROUND: Tropane alkaloids are well known to play a role in plant defense. By blocking acetylcholine receptors, they exert insecticidal and deterrent effects against herbivore insects. Carbamates are an important class of chemical insecticides that also inhibit acetyl cholinesterase. The objective of this work was to synthesize a series of tropane alkaloids bearing a carbamate group, and to evaluate their effects against the pest Ascia monuste. The effects of the most active compounds were evaluated on the A...
March 6, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28261106/a-role-of-corazonin-receptor-in-larval-pupal-transition-and-pupariation-in-the-oriental-fruit-fly-bactrocera-dorsalis-hendel-diptera-tephritidae
#7
Qiu-Li Hou, Hong-Bo Jiang, Shun-Hua Gui, Er-Hu Chen, Dan-Dan Wei, Hui-Min Li, Jin-Jun Wang, Guy Smagghe
Corazonin (Crz) is a neuropeptide hormone, but also a neuropeptide modulator that is internally released within the CNS, and it has a widespread distribution in insects with diverse physiological functions. Here, we identified and cloned the cDNAs of Bactrocera dorsalis that encode Crz and its receptor CrzR. Mature BdCrz has 11 residues with a unique Ser(11) substitution (instead of the typical Asn) and a His in the evolutionary variable position 7. The BdCrzR cDNA encodes a putative protein of 608 amino acids with 7 putative transmembrane domains, typical for the structure of G-protein-coupled receptors...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28260683/endocrine-related-genes-are-altered-by-antibacterial-agent-triclosan-in-chironomus-riparius-aquatic-larvae
#8
Pedro Martínez-Paz, Mónica Morales, Josune Urien, Gloria Morcillo, José Luis Martínez-Guitarte
Triclosan (TCS) is an antibacterial agent widely used in personal care and consumer products and commonly detected in aquatic ecosystems. In the present study, the effects of TCS on endocrine-related genes of Chironomus riparius aquatic larvae, a reference organism in aquatic toxicology, were evaluated. Twenty-four-hour in vivo exposures at 10µg/L, 100µg/L, and 1000µg/L TCS revealed that this xenobiotic was able to alter the transcriptional activity of ecdysone receptor gene (EcR), the ultraspiracle gene (usp), the estrogen-related receptor gene (ERR), and the E74 early ecdysone-inducible gene, as measured by real-time RT-PCR...
March 2, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28223048/vegetative-insecticidal-protein-of-bacillus-thuringiensis-blb459-and-its-efficiency-against-lepidoptera
#9
Hanen Boukedi, Saoussen Ben Khedher, Rania Hadhri, Samir Jaoua, Slim Tounsi, Lobna Abdelkefi-Mesrati
Bacillus thuringiensis strain BLB459 supernatant showed a promising activity against Lepidopteran pests with extremely damages in the larvae midgut. Investigations of the genes that encode secreted toxin demonstrated that this strain harbored a vip3-type gene named vip3(459). Based on its original nucleotide and amino acid sequences, this gene was cloned into pET-14b vector and overexpressed in Escherichia coli. The expressed protein was purified and tested against different insects and interestingly the novel toxin demonstrated a remarkable activity against the stored products pest Ephestia kuehniella and the polyphagous insects Spodoptera littoralis and Agrotis segetum...
February 20, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28185941/expression-map-of-a-complete-set-of-gustatory-receptor-genes-in-chemosensory-organs-of-bombyx-mori
#10
Huizhen Guo, Tingcai Cheng, Zhiwei Chen, Liang Jiang, Youbing Guo, Jianqiu Liu, Shenglong Li, Kiyoko Taniai, Kiyoshi Asaoka, Keiko Kadono-Okuda, Kallare P Arunkumar, Jiaqi Wu, Hirohisa Kishino, Huijie Zhang, Rakesh K Seth, Karumathil P Gopinathan, Nicolas Montagné, Emmanuelle Jacquin-Joly, Marian R Goldsmith, Qingyou Xia, Kazuei Mita
Most lepidopteran species are herbivores, and interaction with host plants affects their gene expression and behavior as well as their genome evolution. Gustatory receptors (Grs) are expected to mediate host plant selection, feeding, oviposition and courtship behavior. However, due to their high diversity, sequence divergence and extremely low level of expression it has been difficult to identify precisely a complete set of Grs in Lepidoptera. By manual annotation and BAC sequencing, we improved annotation of 43 gene sequences compared with previously reported Grs in the most studied lepidopteran model, the silkworm, Bombyx mori, and identified 7 new tandem copies of BmGr30 on chromosome 7, bringing the total number of BmGrs to 76...
March 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28067269/proteomic-analysis-of-cry2aa-binding-proteins-and-their-receptor-function-in-spodoptera-exigua
#11
Lin Qiu, Boyao Zhang, Lang Liu, Weihua Ma, Xiaoping Wang, Chaoliang Lei, Lizhen Chen
The bacterium Bacillus thuringiensis produces Crystal (Cry) proteins that are toxic to a diverse range of insects. Transgenic crops that produce Bt Cry proteins are grown worldwide because of their improved resistance to insect pests. Although Bt "pyramid" cotton that produces both Cry1A and Cry2A is predicted to be more resistant to several lepidopteran pests, including Spodoptera exigua, than plants that produce Cry1Ac alone, the mechanisms responsible for the toxicity of Cry2Aa in S. exigua are not well understood...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28059141/the-molecular-sensory-machinery-of-a-chagas-disease-vector-expression-changes-through-imaginal-moult-and-sexually-dimorphic-features
#12
Jose Manuel Latorre-Estivalis, Hugh M Robertson, Kimberly K O Walden, Jerônimo Ruiz, Leilane Oliveira Gonçalves, Alessandra A Guarneri, Marcelo Gustavo Lorenzo
The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5(th) instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043334/ryanodine-receptor-genes-of-the-rice-stem-borer-chilo-suppressalis-molecular-cloning-alternative-splicing-and-expression-profiling
#13
Y C Peng, C W Sheng, John E Casida, C Q Zhao, Z J Han
The ryanodine receptor (RyR) of the calcium release channel is the main target of anthranilic and phthalic diamide insecticides which have high selective insecticidal activity relative to mammalian toxicity. In this study, the full-length cDNA of Chilo suppressalis RyR (CsRyR) was isolated and characterized. The CsRyR mRNA has an open reading frame (ORF) of 15,387bp nucleotides, which encodes 5128 amino acids with GenBank ID: KR088972. Comparison of protein sequences showed that CsRyR shared high identities with other insects of 77-96% and lower identity to mammals and nematodes with only 42-45%...
January 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/27992549/molecular-characterization-and-functional-analysis-of-a-putative-octopamine-tyramine-receptor-during-the-developmental-stages-of-the-pacific-oyster-crassostrea-gigas
#14
Peng Ji, Fei Xu, Baoyu Huang, Yingxiang Li, Li Li, Guofan Zhang
Octopamine (OA) and its precursor, tyramine (TA), participate in invertebrate development such as growth, maturation, and reproduction by activating their corresponding G protein-coupled receptors (GPCRs). Although OA was first discovered in mollusks (octopus), subsequent studies on OA, TA and related receptors have primarily been conducted in Ecdysozoa, especially in insects. Accordingly, only limited reports on OA/TA receptors in mollusks are available and their physiological roles remain unclear. Here, a full-length cDNA encoding a putative 524 amino acid OA/TA receptor (CgGPR1) was isolated from the Pacific oyster Crassostrea gigas...
2016: PloS One
https://www.readbyqxmd.com/read/27986933/the-spodoptera-exigua-lepidoptera-noctuidae-abcc2-mediates-cry1ac-cytotoxicity-and-in-conjunction-with-cadherin-contributes-to-enhance-cry1ca-toxicity-in-sf9-cells
#15
Xiang-Liang Ren, Wei-Li Jiang, Ya-Jie Ma, Hong-Yan Hu, Xiao-Yan Ma, Yan Ma, Guo-Qing Li
In insects, the mode of Cry1A toxins action has been studied in detail and many receptors that participate in the process are known. Recent evidence has revealed that an ABC transporter (ABCC2) is involved in conferring resistance to Cry1A toxins and that ABCC2 could be a receptor of Cry1A. However, it is not known whether Cry1Ca interacts with the same receptor proteins as Cry1A. In this study, we report the cloning of an ABC transporter gene, SeABCC2b, from the midgut of Spodoptera exigua (Hübner) larvae, and its expression in Sf9 cells for a functional analysis...
December 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27982028/a-molecular-and-neuronal-basis-for-amino-acid-sensing-in-the-drosophila-larva
#16
Vincent Croset, Michael Schleyer, J Roman Arguello, Bertram Gerber, Richard Benton
Amino acids are important nutrients for animals, reflected in conserved internal pathways in vertebrates and invertebrates for monitoring cellular levels of these compounds. In mammals, sensory cells and metabotropic glutamate receptor-related taste receptors that detect environmental sources of amino acids in food are also well-characterised. By contrast, it is unclear how insects perceive this class of molecules through peripheral chemosensory mechanisms. Here we investigate amino acid sensing in Drosophila melanogaster larvae, which feed ravenously to support their rapid growth...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27918112/validation-of-adam10-metalloprotease-as-a-bacillus-thuringiensis-cry3aa-toxin-functional-receptor-in-colorado-potato-beetle-leptinotarsa-decemlineata
#17
V M Ruiz-Arroyo, I García-Robles, C Ochoa-Campuzano, G A Goig, E Zaitseva, G Baaken, A C Martínez-Ramírez, C Rausell, M D Real
Bacillus thuringiensis parasporal crystal proteins (Cry proteins) are insecticidal pore-forming toxins that bind to specific receptor molecules on the brush border membrane of susceptible insect midgut cells to exert their toxic action. In the Colorado potato beetle (CPB), a coleopteran pest, we previously proposed that interaction of Cry3Aa toxin with a CPB ADAM10 metalloprotease is an essential part of the mode of action of this toxin. Here, we annotated the gene sequence encoding an ADAM10 metalloprotease protein (CPB-ADAM10) in the CPB genome sequencing project, and using RNA interference gene silencing we demonstrated that CPB-ADAM10 is a Cry3Aa toxin functional receptor in CPB...
December 5, 2016: Insect Molecular Biology
https://www.readbyqxmd.com/read/27914919/expressing-a-moth-abcc2-gene-in-transgenic-drosophila-causes-susceptibility-to-bt-cry1ac-without-requiring-a-cadherin-like-protein-receptor
#18
Tristan Stevens, Sisi Song, John B Bruning, Amanda Choo, Simon W Baxter
Bt toxins ingested by insect pests can bind to midgut receptors and cause death, although several steps in this process remain unclear. Multiple Bt toxin receptors have been identified in Lepidoptera, including a cadherin-like protein (CaLP), which is central to several models explaining Bt toxins' mode of action. Mutations in the Plutella xylostella ATP-dependent binding cassette transporter C2 (Px-abcc2), rather than CaLP, are genetically linked with Bt Cry1Ac resistance. Here we expressed Px-abcc2 in Drosophila and performed larval bioassays to determine whether this protein acts as an effective Bt receptor...
January 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27908826/itraq-based-quantitative-proteomic-analysis-of-midgut-in-silkworm-infected-with-bombyx-mori-cytoplasmic-polyhedrosis-virus
#19
Kun Gao, Xiang-Yuan Deng, Meng-Ke Shang, Guang-Xing Qin, Cheng-Xiang Hou, Xi-Jie Guo
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) specifically infects the epithelial cells in the midgut of silkworm and causes them to death, which negatively affects the sericulture industry. In order to determine the midgut response at the protein levels to the virus infection, differential proteomes of the silkworm midgut responsive to BmCPV infection were identified with isobaric tags for relative and absolute quantitation (iTRAQ) labeling followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27883266/silencing-of-ecdysone-receptor-insect-intestinal-mucin-and-sericotropin-genes-by-bacterially-produced-double-stranded-rna-affects-larval-growth-and-development-in-plutella-xylostella-and-helicoverpa-armigera
#20
B Israni, M V Rajam
RNA interference mediated gene silencing, which is triggered by double-stranded RNA (dsRNA), has become a important tool for functional genomics studies in various systems, including insects. Bacterially produced dsRNA employs the use of a bacterial strain lacking in RNaseIII activity and harbouring a vector with dual T7 promoter sites, which allow the production of intact dsRNA molecules. Here, we report an assessment of the functional relevance of the ecdysone receptor, insect intestinal mucin and sericotropin genes through silencing by dsRNA in two lepidopteran insect pests, Helicoverpa armigera and Plutella xylostella, both of which cause serious crop losses...
November 24, 2016: Insect Molecular Biology
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