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Receptors insects larvae

Tae-Kyun Hong, Haribalan Perumalsamy, Kyoung-Hwa Jang, Eun-Shik Na, Young-Joon Ahn
Bradysia procera is a serious insect pest of Panax ginseng plants. This study was conducted to determine the toxicity and mechanism of action of three phenylpropanoids, three terpenoids, and a ketone from Syzygium aromaticum bud methanol extract and hydrodistillate against third-instar larvae and eggs of B. procera. In a filter-paper mortality bioassay, methyl salicylate (LC50 , 5.26μg/cm2 ) was the most toxic compound, followed by 2-nonanone, eugenol, and eugenyl acetate (8.77-15.40μg/cm2 ). These compounds were significantly less toxic than either thiamethoxam, clothianidin, or cypermethrin...
February 2018: Pesticide Biochemistry and Physiology
Giorgia Sollai, Maurizio Biolchini, Roberto Crnjar
In the peripheral taste system of insects, the responsiveness of gustatory receptor neurons (GRNs) depends on several factors, such as larval instar, feeding history, physiological state and time of day. To study the role of the feeding history, the spike activity of the maxillary taste chemosensilla in the larvae of two related species of Lepidoptera (Papilio machaon L. and Papilio hospiton Géné) raised on different host plants, was recorded with electrophysiological techniques after stimulation with simple stimuli (sugars, bitters and inorganic salt) and host plant saps, with the aim of cross-comparing their response patterns and evaluating any effects of different feeding histories...
February 15, 2018: Journal of Insect Physiology
Yang Cheng, Zhe Lin, Ju-Mei Wang, Long-Sheng Xing, Guang-Hua Xiong, Zhen Zou
C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was found to form aggregates with zymosan and B. bassiana in vitro. Immunoprecipitation studies demonstrated that CTL14 interacted with serine proteinases (SP), serine proteinase inhibitor (serpin), prophenoloxidases (PPO) and vitellogenin (Vg) in the larval hemolymph...
February 14, 2018: Developmental and Comparative Immunology
Dolors Bosch, Marcela A Rodríguez, Laura Depalo, Jesús Avilla
The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is the key pest on pome fruit and walnut orchards worldwide. Its resistance to available insecticides has been widely reported. Chlorantraniliprole is an anthranilic diamide that was introduced in European countries in 2008-2009 and acts by activating the insect's ryanodine receptors. The aims of this study were to determine the baseline susceptibility of European populations of C. pomonella to chlorantraniliprole, to establish the discriminant concentrations (DC) to check the possible development of resistance, and to know the role of cytochrome P450 monooxygenases (P450) in the possible susceptibility decrease of field populations to the insecticide...
February 9, 2018: Journal of Economic Entomology
Do-Hyoung Kim, Young-Joon Kim, Michael E Adams
Fluid clearance from the respiratory system during developmental transitions is critically important for achieving optimal gas exchange in animals. During insect development from embryo to adult, airway clearance occurs episodically each time the molt is completed by performance of the ecdysis sequence, coordinated by a peptide-signaling cascade initiated by ecdysis-triggering hormone (ETH). We find that the neuropeptide Kinin (also known as Drosokinin or Leukokinin) is required for normal respiratory fluid clearance or "tracheal air-filling" in Drosophila larvae...
February 13, 2018: Proceedings of the National Academy of Sciences of the United States of America
Bharat Nareshkumar, Shaik Mohammad Akbar, Hari Chand Sharma, Senigala K Jayalakshmi, Kuruba Sreeramulu
Neonicotinoids have high agonistic affinity to insect nicotinic acetylcholine receptors (nAChR) and are frequently used as insecticides against most devastating lepidopteran insect pests. Imidacloprid influenced dose-dependent decline in the state III and IV respiration, respiration control index (RCI), and P/O ratios, in vitro and in vivo. The bioassay indicated its LD50 value to be 531.24 μM. The insecticide exhibited a dose-dependent inhibition on F0F1-ATPase and complex IV activity. At 600 μM, the insecticide inhibited 83...
January 5, 2018: Journal of Bioenergetics and Biomembranes
Zulian Liu, Lin Ling, Jun Xu, Baosheng Zeng, Yongping Huang, Peng Shang, Anjiang Tan
MicroRNAs (miRNA) regulate multiple physiological processes including development and metamorphosis in insects. In the current study, we demonstrate that a conserved invertebrate miRNA-14 (miR-14) plays an important role in ecdysteroid regulated development in the silkworm Bombyx mori, a lepidopteran model insect. Ubiquitous transgenic overexpression of miR-14 using the GAL4/UAS system resulted in delayed silkworm larval development and smaller body size of larva and pupa with decrease in ecdysteriod titers...
February 2018: Insect Biochemistry and Molecular Biology
Masasuke Ryuda, Miku Tabuchi, Hitoshi Matsumoto, Takashi Matsumura, Masanori Ochiai, Yoichi Hayakawa
Recovery from weight loss after stress is important for all organisms, although the recovery mechanisms are not fully understood. We are working to clarify these mechanisms. Here, we recorded enhanced feeding activity of Drosophila melanogaster larvae from 2 to 4 h after heat stress at 35°C for 1 h. During the post-stress period, expression levels of sweet taste gustatory receptor genes (Grs), Gr5a, Gr43a, Gr64a, and Gr64f, were elevated, whereas bitter taste Grs, Gr66a, and Gr33a, were decreased in expression and expression of a non-typical taste receptor Gr, Gr68a, was unchanged...
March 2018: Archives of Insect Biochemistry and Physiology
Chuanshan Zou, Guifen Liu, Suning Liu, Shumin Liu, Qisheng Song, Jian Wang, Qili Feng, Yunlin Su, Sheng Li
BACKGROUND: 20-Hydroxyecdysone (20E), a crucial insect steroid hormone, can bind to its cognate nuclear receptor composed of ecdysone receptor (EcR) and ultraspiracle (USP) to activate expression of 20E-response genes, enabling subsequent metamorphosis. In this study, we tried to find out which steroid-like compounds can block insect metamorphosis effectively and provide useful information for biopesticide study. For this purpose, we screened 126 steroid-like compounds for possible 20E antagonists using a dual-luciferase reporter assay with Drosophila melanogaster Kc and Bombyx mori Bm12 cells...
December 4, 2017: Pest Management Science
Fang Zhang, Jun Wang, Kiran Thakur, Fei Hu, Jian-Guo Zhang, Xing-Fu Jiang, Shi-Hen An, Hongbo Jiang, Li Jiang, Zhao-Jun Wei
Insect allatotropin (AT) plays multi-functions including regulation of juvenile hormone synthesis, growth, development and reproduction. In the present study, the full-length cDNA encoding the AT receptor was cloned from the brain of Helicoverpa armigera (Helar-ATR). The ORF of Helar-ATR exhibited the characteristic seven transmembrane domains of the G protein-coupled receptor (GPCR) and was close to the ATR of Manduca sexta in the phylogenetic tree. The Helar-ATR expressed in vertebrate cell lines can be activated by Helar-AT and each Helar-ATL in a dose-responsive manner, in the following order: Helar-ATLI>Helar-ATLII>Helar-AT>Helar-ATLIII...
December 2, 2017: Peptides
Laila Gasmi, Agata K Jakubowska, Juan Ferré, Mylène Ogliastro, Salvador Herrero
Insect innate immunity relies on numerous soluble and membrane-bound receptors, named pattern recognition proteins (PRPs), which enable the insect to recognize pathogen-associated molecular patterns. C-type lectins are among the best-studied PRPs and constitute the most diverse family of animal lectins. Here we have characterized two groups of Spodoptera exigua C-type lectins that differ in their phylogeny, domain architecture, and expression pattern. One group includes C-type lectins with similar characteristics to other lepidopteran lectins, and a second group includes bracoviral-related lectins (bracovirus-like lectins, Se-BLLs) recently acquired by horizontal gene transfer...
November 22, 2017: Archives of Insect Biochemistry and Physiology
Yong-Bo Li, Xu-Yang Pei, Di Wang, Cai-Hua Chen, Mei-Juan Cai, Jin-Xing Wang, Xiao-Fan Zhao
Animal steroid hormones stimulate extracellular Ca(2+) influx into cells; however, the mechanism remains unclear. In this study, we determined that the Ca(2+) influx induced by steroid hormone 20-hydroxyecdysone (20E) is mediated by the calcium release-activated calcium channel modulator 1 (CRACM1/Orai1). The Orai1 mRNA is highly expressed during midgut programmed cell death in the lepidopteran insect Helicoverpa armigera. 20E upregulated the expression of Orai1 in H. armigera larvae and in an epidermal cell line (HaEpi)...
December 2017: Cell Calcium
Lei Guo, Pei Liang, Kuan Fang, Dong Chu
Cyantraniliprole is the second active ingredient of anthranilic diamide insecticide, and the first to control a cross-spectrum of chewing and sucking pests such as sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). The inositol 1,4,5-trisphosphate receptor (IP3R) and ryanodine receptor (RyR) are two families of Ca(2+) release channels to raise the cytoplasmic free calcium concentration when it is activated by various extracellular stimuli. Previous study proved the over-expression of ryanodine receptor (RyR) was associated with the resistance to diamide insecticides, while the roles of IP3R in diamide resistance remain unknown...
October 2017: Pesticide Biochemistry and Physiology
Adriana Seixas, María Fernanda Alzugaray, Lucas Tirloni, Luis Fernando Parizi, Antonio Frederico Michel Pinto, Naftaly Wang'ombe Githaka, Satoru Konnai, Kazuhiko Ohashi, John R Yates Iii, Carlos Termignoni, Itabajara da Silva Vaz
The vitellogenin receptor (VgR), which belongs to the low-density lipoprotein receptors (LDLR) family, regulates the absorption of yolk protein accumulated in developing oocytes during oogenesis. In the present study, the full sequence of Rhipicephalus microplus VgR (RmVgR) and the partial sequence of Rhipicephalus appendiculatus VgR (RaVgR) ORF were determined and cloned. The RmVgR amino acid sequence contains the five highly conserved structural motifs characteristic of LDLR superfamily members, the same overall structure as observed in other species...
October 16, 2017: Ticks and Tick-borne Diseases
Taoyan Wu, Ya Zhao, Zhenying Wang, Qisheng Song, Zengxia Wang, Qiuwen Xu, Yingjuan Wang, Libao Wang, Yiqiang Zhang, Congjing Feng
Pattern recognition receptors (PRRs) are biosensor proteins that bind to non-self pathogen associated molecular patterns (PAMPs). β-1,3-glucan recognition proteins (βGRPs) play an essential role in immune recognition and signaling pathway of insect innate immunity. Here, we report the cloning and characterization of cDNA of OfβGRP3 from Ostrinia furnacalis larvae. The OfβGRP3 contains 1455 bp open reading frame, encoding a predicted 484 amino acid residue protein. In hemocytes, the expression levels of OfβGRP3 in Escherichia coli-challenged group were higher than those of Bacillus subtilis-challenged group at 2, 4, 8, 10 and 12 h post injection (HPI)...
October 12, 2017: Developmental and Comparative Immunology
June-Sun Yoon, Dhandapani Gurusamy, Subba Reddy Palli
RNA interference (RNAi) efficiency varies among insects studied. The barriers for successful RNAi include the presence of double-stranded ribonucleases (dsRNase) in the lumen and hemolymph that could potentially digest double-stranded RNA (dsRNA) and the variability in the transport of dsRNA into and within the cells. We recently showed that the dsRNAs are transported into lepidopteran cells, but they are not processed into small interference RNAs (siRNAs) because they are trapped in acidic bodies. In the current study, we focused on the identification of acidic bodies in which dsRNAs accumulate in Sf9 cells...
November 2017: Insect Biochemistry and Molecular Biology
Yan Shi, Hong-Bo Jiang, Shun-Hua Gui, Xiao-Qiang Liu, Yu-Xia Pei, Li Xu, Guy Smagghe, Jin-Jun Wang
Insects must undergo ecdysis for successful development and growth, and the ecdysis triggering hormone (ETH), released by the Inka cells, is a master hormone in this process. In this study, we determined the sequence of the ETH precursor and receptors in an agriculturally important pest insect, the oriental fruit fly Bactrocera dorsalis (Hendel). We identified two functionally distinct splice receptor isoforms: BdETH-R-A and BdETH-R-B, and when expressed in Chinese hamster ovary (CHO-WTA11) cells, they exhibited a high sensitivity to the two mature peptides BdETH1 and BdETH2...
2017: Frontiers in Physiology
Soo Gon Kim, Yong Hun Jo, Jeong Hwan Seong, Ki Beom Park, Mi Young Noh, Jun Ho Cho, Hye Jin Ko, Chang Eun Kim, Hamisi Tindwa, Bharat Bhusan Patnaik, In Seok Bang, Yong Seok Lee, Yeon Soo Han
Scavenger receptors (SRs) constitute a family of membrane-bound receptors that bind to multiple ligands. The SR family of proteins is involved in removing cellular debris, oxidized low-density lipoproteins, and pathogens. Specifically, class C scavenger receptors (SR-C) have also been reported to be involved in phagocytosis of gram-positive and -negative bacteria in Drosophila and viruses in shrimp. However, reports are unavailable regarding the role of SR-C in antifungal immune mechanisms in insects. In this study, a full-length Tenebrio molitor SR-C (TmSR-C) sequence was obtained by 5'- and 3'-Rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR)...
October 2017: Insect Biochemistry and Molecular Biology
Bembem Thounaojam, Bela Keshan
Insect growth and development are mainly regulated via synchronization of many extrinsic and intrinsic factors such as nutrition and hormones. Previously we have demonstrated that larval growth period influences the effect of insulin on the accumulation of glycogen in the fat body of Bombyx larvae. In the present study we demonstrate that Bombyx larvae at the terminal growth period (TGP, after critical weight) had a significantly greater increase in the expression level of Akt in the fat body than at the active growth period (AGP, before critical weight)...
November 2017: Gene Expression Patterns: GEP
Zhangfei Shen, Yu Chen, Lingjuan Hong, Zhenteng Cui, Huipeng Yang, Xiaobai He, Ying Shi, Liangen Shi, Feng Han, Naiming Zhou
CAPA peptides, such as periviscerokinin (PVK), are insect neuropeptides involved in many signaling pathways controlling, for example, metabolism, behavior, and reproduction. They are present in a large number of insects and, together with their cognate receptors, are important for research into approaches for improving insect control. However, the CAPA receptors in the silkworm (Bombyx mori) insect model are unknown. Here, we cloned cDNAs of two putative CAPA peptide receptor genes, BNGR-A27 and -A25, from the brain of B...
October 6, 2017: Journal of Biological Chemistry
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