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https://www.readbyqxmd.com/read/29054608/a-sweet-spot-for-fluid-induced-oscillations-in-the-conditioning-of-stem-cell-based-engineered-heart-valve-tissues
#1
Alexander Williams, Sana Nasim, Manuel Salinas, Arash Moshkforoush, Nikolaos Tsoukias, Sharan Ramaswamy
Fluid-induced shear stresses are involved in the development of cardiovascular tissues. In a tissue engineering framework, this stimulus has also been considered as a mechanical regulator of stem cell differentiation. We recently demonstrated that the fluid-oscillating effect in combination with a physiologically-relevant shear stress magnitude contributes to the formation of stem cell-derived de novo heart valve tissues. However, the range of oscillations necessary to induce favorable gene expression and engineered tissue formation is unknown...
October 7, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29053925/mesenchymal-stem-cell-secretome-improves-tendon-cell-viability-in-vitro-and-tendon-bone-healing-in-vivo-when-a-tissue-engineering-strategy-is-used-in-a-rat-model-of-chronic-massive-rotator-cuff-tear
#2
Nuno Sevivas, Fábio Gabriel Teixeira, Raquel Portugal, Bruno Direito-Santos, João Espregueira-Mendes, Filipe J Oliveira, Rui F Silva, Nuno Sousa, Wan Ting Sow, Nguyen Luong, Kee Woei Ng, António J Salgado
BACKGROUND: Massive rotator cuff tears (MRCTs) represent a major clinical concern, especially when degeneration and chronicity are involved, which highly compromise healing capacity. PURPOSE: To study the effect of the secretome of mesenchymal stem cells (MSCs) on tendon cells (TCs) followed by the combination of these activated TCs with an electrospun keratin-based scaffold to develop a tissue engineering strategy to improve tendon-bone interface (TBi) healing in a chronic MRCT rat model...
October 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29048769/in-vivo-implantation-of-a-tissue-engineered-stem-cell-seeded-hemi-laryngeal-replacement-maintains-airway-phonation-and-swallowing-in-pigs
#3
P Herrmann, T Ansari, A Southgate, A Varanou Jenkins, L Partington, C Carvalho, S Janes, M Lowdell, P Sibbons, M A Birchall
BACKGROUND: Laryngeal functional impairment relating to swallowing, vocalisation and respiration can be life changing and devastating for patients. A tissue engineering approach to regenerating vocal folds would represent a significant advantage over current clinical practice. METHOD: Porcine hemi-larynx were de-cellularised under negative pressure. The resultant acellular scaffold was seeded with human bone marrow derived mesenchymal stem cells and primary human epithelial cells...
October 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29046717/designing-of-pla-scaffolds-for-bone-tissue-replacement-fabricated-by-ordinary-commercial-3d-printer
#4
Aleš Gregor, Eva Filová, Martin Novák, Jakub Kronek, Hynek Chlup, Matěj Buzgo, Veronika Blahnová, Věra Lukášová, Martin Bartoš, Alois Nečas, Jan Hošek
BACKGROUND: The primary objective of Tissue engineering is a regeneration or replacement of tissues or organs damaged by disease, injury, or congenital anomalies. At present, Tissue engineering repairs damaged tissues and organs with artificial supporting structures called scaffolds. These are used for attachment and subsequent growth of appropriate cells. During the cell growth gradual biodegradation of the scaffold occurs and the final product is a new tissue with the desired shape and properties...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/29045803/chitosan-based-trilayer-scaffold-for-multitissue-periodontal-regeneration
#5
E M Varoni, S Vijayakumar, E Canciani, A Cochis, L De Nardo, G Lodi, L Rimondini, M Cerruti
Periodontal regeneration is still a challenge for periodontists and tissue engineers, as it requires the simultaneous restoration of different tissues-namely, cementum, gingiva, bone, and periodontal ligament (PDL). Here, we synthetized a chitosan (CH)-based trilayer porous scaffold to achieve periodontal regeneration driven by multitissue simultaneous healing. We produced 2 porous compartments for bone and gingiva regeneration by cross-linking with genipin either medium molecular weight (MMW) or low molecular weight (LMW) CH and freeze-drying the resulting scaffolds...
October 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29044984/3d-printed-calcium-phosphate-and-poly-caprolactone-composites-with-improved-mechanical-properties-and-preserved-microstructure
#6
Joseph B Vella, Ryan P Trombetta, Michael D Hoffman, Jason Inzana, Hani Awad, Danielle S W Benoit
Biphasic calcium phosphate scaffolds formed via 3D printing technology to exhibit porosity and chemical resorbability to promote osseointegration often lack the strength and toughness required to withstand loading in bone tissue engineering applications. Herein, sintering and CaP:poly(caprolactone) (PCL) composite formation were explored to improve 3D printed scaffold strength and toughness. Hydroxyapatite and α-tricalcium phosphate (α-TCP) biphasic calcium powders were printed using phosphoric acid binder, which generated monetite and hydroxyapatite scaffolds...
October 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29042614/3d-printed-poly-%C3%AE%C2%B5-caprolactone-scaffold-integrated-with-cell-laden-chitosan-hydrogels-for-bone-tissue-engineering
#7
Liang Dong, Shao-Jie Wang, Xin-Rong Zhao, Yu-Fang Zhu, Jia-Kuo Yu
Synthetic polymeric scaffolds are commonly used in bone tissue engineering (BTE) due to their biocompatibility and adequate mechanical properties. However, their hydrophobicity and the lack of specific cell recognition sites confined their practical application. In this study, to improve the cell seeding efficiency and osteoinductivity, an injectable thermo-sensitive chitosan hydrogel (CSG) was incorporated into a 3D-printed poly(ε-caprolactone) (PCL) scaffold to form a hybrid scaffold. To demonstrate the feasibility of this hybrid system for BTE application, rabbit bone marrow mesenchymal stem cells (BMMSCs) and bone morphogenetic protein-2 (BMP-2) were encapsulated in CSG...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042571/a-novel-technique-for-accelerated-culture-of-murine-mesenchymal-stem-cells-that-allows-for-sustained-multipotency
#8
Courtney M Caroti, Hyunhee Ahn, Hector F Salazar, Giji Joseph, Sitara B Sankar, Nick J Willett, Levi B Wood, W Robert Taylor, Alicia N Lyle
Bone marrow derived mesenchymal stem cells (MSCs) are regularly utilized for translational therapeutic strategies including cell therapy, tissue engineering, and regenerative medicine and are frequently used in preclinical mouse models for both mechanistic studies and screening of new cell based therapies. Current methods to culture murine MSCs (mMSCs) select for rapidly dividing colonies and require long-term expansion. These methods thus require months of culture to generate sufficient cell numbers for feasibility studies in a lab setting and the cell populations often have reduced proliferation and differentiation potential, or have become immortalized cells...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29039747/development-of-useful-biomaterial-for-bone-tissue-engineering-by-incorporating-nano-copper-zinc-alloy-ncuzn-in-chitosan-gelatin-nano-hydroxyapatite-ch-g-nhap-scaffold
#9
Juan Carlos Forero, Eduardo Roa, Juan G Reyes, Cristian Acevedo, Nelson Osses
Ceramic and metallic nanoparticles can improve the mechanical and biological properties of polymeric scaffolds for bone tissue engineering (BTE). In this work, nanohydroxyapatite (nHAp) and nano-copper-zinc alloy (nCuZn) were added to a chitosan/gelatin (Ch/G) scaffold in order to investigate the effects on morphological, physical, and biocompatibility properties. Scaffolds were fabricated by a freeze-drying technique using different pre-freezing temperatures. Microstructure and morphology were studied by scanning electron microscopy (SEM), glass transition (Tg) was studied using differential scanning calorimetry (DSC), cell growth was estimated by MTT assay, and biocompatibility was examined in vitro and in vivo by histochemistry analyses...
October 17, 2017: Materials
https://www.readbyqxmd.com/read/29038440/mir-29b-regulates-expression-of-collagens-i-and-iii-in-chondrogenically-differentiating-bmsc-in-an-osteoarthritic-environment
#10
Ute Mayer, Achim Benditz, Susanne Grässel
Osteoarthritis (OA) is characterized by a slowly progressing, irreversible loss of articular cartilage. Tissue engineering approaches for cartilage regeneration include stem cell-based strategies but not much is known about their repair capacity in an OA microenvironment. The aim of the present study was to identify factors regulating collagen expression during chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSC) in an OA microenvironment. Coculture with OA cartilage induced miR-29b expression in BMSC which inhibited collagen I and III expression...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29037044/porous-pcl-plla-semi-ipns-as-superior-defect-specific-scaffolds-with-potential-for-cranial-bone-defect-repair
#11
Lindsay N Woodard, Kevin T Kmetz, Abigail A Roth, Vanessa M Page, Melissa A Grunlan
The treatment of irregular cranial bone defects is currently limited due to the graft resorption that can occur when an ill-fitting interface exists between an autograft and the surrounding tissue. A tissue engineering scaffold able to achieve defect-specific geometries could improve healing. This work reports a macroporous, shape memory polymer (SMP) scaffold composed of a semi-interpenetrating network (semi-IPN) of thermoplastic poly(L-lactic acid) (PLLA) within cross-linked poly(ε-caprolactone) diacrylate (PCL-DA) that is capable of conformal fit within a defect...
October 16, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29036882/biomineralization-of-fucoidan-peptide-blends-and-their-potential-applications-in-bone-tissue-regeneration
#12
Harrison T Pajovich, Ipsita A Banerjee
Fucoidan (Fuc), a natural polysaccharide derived from brown seaweed algae, and gelatin (Gel) were conjugated to form a template for preparation of biomimetic scaffolds for potential applications in bone tissue regeneration. To the Fuc-Gel we then incorporated the peptide sequence MTNYDEAAMAIASLN (MTN) derived from the E-F hand domain, known for its calcium binding properties. To mimic the components of the extracellular matrix of bone tissue, the Fuc-Gel-MTN assemblies were incubated in simulated body fluid (SBF) to induce biomineralization, resulting in the formation of β-tricalcium phosphate, and hydroxyapatite (HAp)...
September 20, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/29036390/endothelial-cells-modified-by-adenovirus-vector-containing-nine-copies-hypoxia-response-elements-and-human-vascular-endothelial-growth-factor-as-the-novel-seed-cells-for-bone-tissue-engineering
#13
Xiaobin Song, Liang Shi, Lamei Chen, Xinyu Liu, Xun Qu, Ketao Wang, Fengcai Wei
Vascularization is one of the hotspots during the development of new therapeutic strategies for bone tissue engineering, which can alleviate hypoxic circumstance and prevent transplant failure. Vascular endothelial growth factor (VEGF) gene transfection using recombinant adenovirus (Ad) vector can effectively promote angiogenesis, but uncontrolled long-term continuous expression of VEGF brings safety concern. Here we constructed a recombinant Ad vector containing nine copies of HRE promoter and the hVEGF165 gene, which conserved the oxygen sensitivity of hypoxia-inducible factor-1/hypoxia response elements (HIF-1/HRE)...
September 27, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29035127/healing-potential-of-nanohydroxyapatite-gelatin-and-fibrin-platelet-glue-combination-as-tissue-engineered-scaffolds-in-radial-bone-defects-of-rats
#14
Abdolhamid Meimandi-Parizi, Ahmad Oryan, Hojjat Gholipour
Different biomaterials have been used in orthopedic surgery. Evaluation of biomaterials for bone healing promotion has been a wide area of research of the orthopedic field. Sixty critical size defects of 5 mm long were bilaterally created in the radial diaphysis of 30 rats. The animals were randomly divided into six equal groups as empty defect, autograft, nanohydroxyapatite (nHA), Gelatin (Gel)-nHA, Fibrin-platelet glue (FPG)-nHA and Gel-FPG-nHA groups (n = 10 in each group). Radiographs of each forelimb were taken postoperatively on the 1(st) day and then at the 28(th) and 56(th) days post injury...
October 16, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29027958/adipose-tissue-as-a-strategic-source-of-mesenchymal-stem-cells-in-bone-regeneration-a-topical-review-on-the-most-promising-craniomaxillofacial-applications
#15
REVIEW
Francesco Paduano, Massimo Marrelli, Massimiliano Amantea, Carlo Rengo, Sandro Rengo, Michel Goldberg, Gianrico Spagnuolo, Marco Tatullo
Bone regeneration in craniomaxillofacial surgery represents an issue that involves both surgical and aesthetic aspects. The most recent studies on bone tissue engineering involving adipose-derived stromal/stem cells (ASCs) have clearly demonstrated that such cells can play a crucial role in the treatment of craniomaxillofacial defects, given their strong commitment towards the osteogenic phenotype. A deeper knowledge of the molecular mechanisms underlying ASCs is crucial for a correct understanding of the potentialities of ASCs-based therapies in the most complex maxillofacial applications...
October 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29027611/preparation-of-flexible-bone-tissue-scaffold-utilizing-sea-urchin-test-and-collagen
#16
Naga Vijaya Lakshmi Manchinasetty, Sho Oshima, Masanori Kikuchi
Gonads of sea urchin are consumed in Japan and some countries as food and most parts including its tests are discarded as marine wastes. Therefore, utilization of them as functional materials would reduce the waste as well as encourage Japanese fishery. In this study, magnesium containing calcite granules collected from sea urchin tests were hydrothermally phosphatized and the obtained granules were identified as approximately 82% in mass of magnesium containing β-tricalcium phosphate and 18% in mass of nonstoichiometric hydroxyapatite, i...
October 13, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29027158/isq-calculation-evaluation-of-in-vitro-laser-scanning-vibrometry-captured-resonance-frequency
#17
Stijn Debruyne, Nicolas Grognard, Gino Verleye, Korneel Van Massenhove, Dimitrios Mavreas, Bart Vande Vannet
BACKGROUND: Implant stability testing at various stages of implant therapy by means of resonance frequency analysis is extensively used. The overall measurement outcome is a function of the resulting stiffness of three entities: surrounding bone, bone-implant complex, and implant-Smartpeg complex. The influence of the latter on the overall measurement results is presently unknown. It can be investigated in vitro by use of imbedded implants with mounted Smartpegs. This enables to keep the influence of the two other entities constant and controlled...
October 12, 2017: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/29026644/wet-spinning-fabrication-of-shear-patterned-alginate-hydrogel-microfibers-and-the-guidance-of-cell-alignment
#18
You Yang, Jing Sun, Xiaolu Liu, Zhenzhen Guo, Yunhu He, Dan Wei, Meiling Zhong, Likun Guo, Hongsong Fan, Xingdong Zhang
Native tissue is naturally comprised of highly-ordered cell-matrix assemblies in a multi-hierarchical way, and the nano/submicron alignment of fibrous matrix is found to be significant in supporting cellular functionalization. In this study, a self-designed wet-spinning device appended with a rotary receiving pool was used to continuously produce shear-patterned hydrogel microfibers with aligned submicron topography. The process that the flow-induced shear force reshapes the surface of hydrogel fiber into aligned submicron topography was systematically analysed...
October 2017: Regenerative Biomaterials
https://www.readbyqxmd.com/read/29025671/in-situ-and-ex-situ-modifications-of-bacterial-cellulose-for-applications-in-tissue-engineering
#19
REVIEW
Taisa Regina Stumpf, Xiuying Yang, Jingchang Zhang, Xudong Cao
Bacterial cellulose (BC) is secreted by a few strains of bacteria and consists of a cellulose nanofiber network with unique characteristics. Because of its excellent mechanical properties, outstanding biocompatibilities, and abilities to form porous structures, BC has been studied for a variety of applications in different fields, including the use as a biomaterial for scaffolds in tissue engineering. To extend its applications in tissue engineering, native BC is normally modified to enhance its properties...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29025657/novel-fluoridated-silk-fibroin-tio2-nanocomposite-scaffolds-for-bone-tissue-engineering
#20
Narges Johari, Hamid Reza Madaah Hosseini, Ali Samadikuchaksaraei
It is known that Fluoride ions strongly affect bone mineralization and formation. In the present study, the engineered bone tissue scaffolds are fabricated using silk fibroin (SF) and flouridated TiO2 nanoparticles. TiO2 nanoparticles are modified by fluoride ions, and different levels (0, 5, 10, 15 and 20wt%) of the fluoridated TiO2 nanoparticles (TiO2-F) were subsequently added to the SF matrix through phase separation method to prepare silk fibroin/flouridated TiO2 nanocomposite scaffolds (SF/TiO2-F). Phase structure, functional groups, morphology and mechanical properties of the obtained scaffolds were evaluated by X-ray diffraction method (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and compressive testing, respectively...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
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