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bone tissue engineering

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https://www.readbyqxmd.com/read/29458214/effect-of-tetrahedral-dna-nanostructures-on-proliferation-and-osteo-odontogenic-differentiation-of-dental-pulp-stem-cells-via-activation-of-the-notch-signaling-pathway
#1
Mi Zhou, Nan-Xin Liu, Si-Rong Shi, Yong Li, Qi Zhang, Quan-Quan Ma, Tao-Ran Tian, Wen-Juan Ma, Xiao-Xiao Cai, Yun-Feng Lin
Dental pulp stem cells (DPSCs) derived from the human dental pulp tissue have multiple differentiation capabilities, such as osteo/odontogenic differentiation. Therefore, DPSCs are deemed as ideal stem cell sources for tissue regeneration. As new nanomaterials based on DNA, tetrahedral DNA nanostructures (TDNs) have tremendous potential for biomedical applications. Here, the authors aimed to explore the part played by TDNs in proliferation and osteo/odontogenic differentiation of DPSCs, and attempted to investigate if these cellular responses could be driven by activating the canonical Notch signaling pathway...
February 16, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29457597/injectable-collagen-rgd-systems-for-bone-tissue-engineering-applications
#2
Fu-Chen Kung
Imbalance crosslink density and polymer concentration gradient is formed within the traditional alginate hydrogel using calcium chloride as a crosslinking agent in external gelation for instantaneously process. In this studying, type I collagen (Col I) blended calcium salt form of poly(γ-glutamic acid) (γCaPGA) was mixing with RGD-modified alginate with convenient gelation process and suitable for practical use. The hydrophilicity of the resulting hydrogels was evaluated through swelling tests, water retention capacity tests, and water vapor permeation tests...
2018: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/29456878/non-destructive-two-photon-excited-fluorescence-imaging-identifies-early-nodules-in-calcific-aortic-valve-disease
#3
Lauren M Baugh, Zhiyi Liu, Kyle P Quinn, Sam Osseiran, Conor L Evans, Gordon S Huggins, Philip W Hinds, Lauren D Black, Irene Georgakoudi
Calcifications occur during the development of healthy bone, and at the onset of calcific aortic-valve disease (CAVD) and many other pathologies. Although the mechanisms regulating early calcium deposition are not fully understood, they may provide targets for new treatments and for early interventions. Here, we show that two-photon excited fluorescence (TPEF) can provide quantitative and sensitive readouts of calcific nodule formation, in particular in the context of CAVD. Specifically, by means of the decomposition of TPEF spectral images from excised human CAVD valves and from rat bone prior to and following demineralization, as well as from calcific nodules formed within engineered gels, we identified an endogenous fluorophore that correlates with the level of mineralization in the samples...
November 2017: Nature Biomedical Engineering
https://www.readbyqxmd.com/read/29456294/cobalt-containing-bioactive-glasses-reduce-human-mesenchymal-stem-cell-chondrogenic-differentiation-despite-hif-1%C3%AE-stabilisation
#4
E Littmann, H Autefage, A K Solanki, C Kallepitis, J R Jones, M Alini, M Peroglio, M M Stevens
Bioactive glasses (BGs) are excellent delivery systems for the sustained release of therapeutic ions and have been extensively studied in the context of bone tissue engineering. More recently, due to their osteogenic properties and expanding application to soft tissue repair, BGs have been proposed as promising materials for use at the osteochondral interface. Since hypoxia plays a critical role during cartilage formation, we sought to investigate the influence of BGs releasing the hypoxia-mimicking agent cobalt (CoBGs) on human mesenchymal stem cell (hMSC) chondrogenesis, as a novel approach that may guide future osteochondral scaffold design...
March 2018: Journal of the European Ceramic Society
https://www.readbyqxmd.com/read/29456164/pluripotent-stem-cells-as-a-source-of-osteoblasts-for-bone-tissue-regeneration
#5
Hui Zhu, Takaharu Kimura, Srilatha Swami, Joy Y Wu
Appropriate and abundant sources of bone-forming osteoblasts are essential for bone tissue engineering. Pluripotent stem cells can self-renew and thereby offer a potentially unlimited supply of osteoblasts, a significant advantage over other cell sources. We generated mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from transgenic mice expressing rat 2.3 kb type I collagen promoter-driven green fluorescent protein (Col2.3GFP), a reporter of the osteoblast lineage. We demonstrated that Col2...
February 5, 2018: Biomaterials
https://www.readbyqxmd.com/read/29453386/anti-fibrotic-effects-of-cxcr4-targeting-i-body-ad-114-in-preclinical-models-of-pulmonary-fibrosis
#6
K Griffiths, D M Habiel, J Jaffar, U Binder, W G Darby, C G Hosking, A Skerra, G P Westall, C M Hogaboam, M Foley
Idiopathic pulmonary fibrosis (IPF) is a chronic fibrotic lung disease that is prevalent in individuals >50 years of age, with a median survival of 3-5 years and limited therapeutic options. The disease is characterized by collagen deposition and remodeling of the lung parenchyma in a process that is thought to be driven by collagen-expressing immune and structural cells. The G-protein coupled C-X-C chemokine receptor 4, CXCR4, is a candidate therapeutic target for IPF owing to its role in the recruitment of CXCR4 + fibrocytes from the bone marrow to fibrotic lung tissue and its increased expression levels by structural cells in fibrotic lung tissue...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29452946/time-responsive-osteogenic-niche-of-stem-cells-a-sequentially-triggered-dual-peptide-loaded-alginate-hybrid-system-for-promoting-cell-activity-and-osteo-differentiation
#7
Zuyuan Luo, Siqi Zhang, Jijia Pan, Rui Shi, Hao Liu, Yalin Lyu, Xiao Han, Yan Li, Yue Yang, Zhixiu Xu, Yi Sui, En Luo, Yuanyuan Zhang, Shicheng Wei
The efficacy of stem cell-based bone tissue engineering has been hampered by cell death and limited fate control. A smart cell culture system with the capability of sequentially delivering multiple factors in specific growth stages, like the mechanism of the natural extracellular matrix modulating tissue formation, is attractive for enhancing cell activity and controlling cell fate. Here, a bone forming peptide-1 (BFP-1)-laden mesoporous silica nanoparticles (pep@MSNs) incorporated adhesion peptide, containing the arginine-glycine-aspartic acid (RGD) domain, modified alginate hydrogel (RA) system (pep@MSNs-RA) was developed to promote the activity and stimulate osteo-differentiation of human mesenchymal stem cells (hMSCs) in sequence...
February 10, 2018: Biomaterials
https://www.readbyqxmd.com/read/29450477/cyclic-compression-of-tissue-engineered-intervertebral-disc-constructs-composed-of-electrospinning-polycaprolactone
#8
Andrea Fotticchia, Emrah Demirci, Cristina Lenardi, Y Liu
There is lack of investigation capturing the complex mechanical interaction of tissue engineered IVD (intervertebral disc) constructs in physiologically-relevant environmental conditions. In this study, mechanical characterisation of anisotropic eletrospinning (ES) substrates made of polycaprolactone (PCL) was carried out in wet and dry conditions and viability of human bone marrow derived mesenchymal stem cells (hMSCs) seeded within double layers of ES PCL was also studied. Cyclic compression of IVD-like constructs composed of an agarose core confined by ES PCL double-layers was implemented using a bioreactor and the cellular response to the mechanical stimulation was evaluated...
February 14, 2018: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/29448837/restoring-tracheal-defects-in-a-rabbit-model-with-tissue-engineered-patches-based-on-tgf-%C3%AE-3-encapsulating-electrospun-poly-l-lactic-acid-co-%C3%AE%C2%B5-caprolactone-collagen-scaffolds
#9
Hui Jing, Botao Gao, Manchen Gao, Haiyue Yin, Xiumei Mo, Xiaoyang Zhang, Kai Luo, Bei Feng, Wei Fu, Jing Wang, Wei Zhang, Meng Yin, Zhongqun Zhu, Xiaomin He, Jinghao Zheng
Long segment tracheal stenosis often has a poor prognosis due to the limited availability of materials for tracheal reconstruction. Tissue engineered tracheal patches based on electrospun scaffolds and stem cells present ideal solutions to this medical challenge. However, the established engineering process is inefficient and time-consuming. In our research, to optimize the engineering process, core-shell nanofilms encapsulating TGF-β3 were fabricated as scaffolds for tracheal patches. The morphological and mechanical characteristics, degradation and biocompatibility of poly(l-lactic acid-co-ε-caprolactone)/collagen (PLCL/collagen) scaffolds with different compositions (PLCL:collagen 75:25, 50:50 and 25:75, respectively) were comparatively evaluated to determine the preferable compositional ratio...
February 15, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29448134/enhancing-proliferation-and-optimizing-the-culture-condition-for-human-bone-marrow-stromal-cells-using-hypoxia-and-fibroblast-growth-factor-2
#10
Jung-Seok Lee, Seul Ki Kim, Byung-Joo Jung, Seong-Bok Choi, Eun-Young Choi, Chang-Sung Kim
This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation...
January 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29446455/uncultivated-stromal-vascular-fraction-is-equivalent-to-adipose-derived-stem-and-stromal-cells-on-porous-polyurethrane-scaffolds-forming-adipose-tissue-in-vivo
#11
Michael Griessl, Anna-Maria Buchberger, Sybille Regn, Kilian Kreutzer, Katharina Storck
OBJECTIVES/HYPOTHESIS: To find an alternative approach to contemporary techniques in tissue augmentation and reconstruction, tissue engineering strategies aim to involve adipose-derived stem and stromal cells (ASCs) harboring a strong differentiation potential into various tissue types such as bone, cartilage, and fat. STUDY DESIGN: Animal research. METHODS: The stromal vascular fraction (SVF) was used directly as a cell source to provide a potential alternative to contemporary ASC-based adipose tissue engineering...
February 15, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29442223/beneficial-role-of-low-intensity-laser-irradiation-on-neural-%C3%AE-tubulin-iii-protein-expression-in-human-bone-marrow-multipotent-mesenchymal-stromal-cells
#12
Valéria Ferreira-Silva, Fernando L Primo, Munira M A Baqui, Danielle A R Magalhães, Maristela D Orellana, Andrielle Castilho-Fernandes, Mario C Cruz, Niehls O S Câmara, Dimas T Covas, Antonio C Tedesco
The purpose of the present study was to evaluate the neural protein expression pattern of human multipotent mesenchymal stromal cells (hMSCs) treated with forskolin (free-form/FF). The study investigated forskolin's capacity to enhance intracellular levels of cyclic adenosine monophosphate (cAMP) by activating adenylate cyclase and probably by inducing neuron-like cells in vitro. In addition, because nanotechnology is a growing field of tissue engineering, we also assessed the action of a new system called the nanostructured-forskolin (NF) to examine the improvement of drug delivery...
February 13, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29442071/differentiation-of-osteoclast-precursors-on-gellan-gum-based-spongy-like-hydrogels-for-bone-tissue-engineering
#13
Raquel Maia, David Musson, Dorit Naot, Lucília Pereira da Silva, Ana R Bastos, João B Costa, Joaquim Miguel Oliveira, Vitor Manuel Correlo, Rui Luis Reis, Jillian Cornish
Bone tissue engineering with cell-scaffold constructs has been attracting a lot of attention, in particular as a tool for efficient guiding of new tissue formation. However, the majority of the current strategies used to evaluate novel biomaterials focus on osteoblasts and bone formation, while osteoclasts are often overlooked. Consequently, there is limited knowledge about the interaction between osteoclasts and biomaterials. In this study, the ability of gellan gum and hydroxyapatite reinforced gellan gum spongy-like hydrogels to support osteoclastogenesis was investigated in vitro...
February 14, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29438883/gradient-nano-engineered-in-situ-forming-composite-hydrogel-for-osteochondral-regeneration
#14
Janani Radhakrishnan, Amrutha Manigandan, Prabu Chinnaswamy, Anuradha Subramanian, Swaminathan Sethuraman
Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging. We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively. Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05). Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs...
February 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29438617/construction-of-bioactive-and-reinforced-bioresorbable-nanocomposites-by-reduced-nano-graphene-oxide-carbon-dots
#15
Nejla B Erdal, Minna Hakkarainen
Bioactive and reinforced poly(ε-caprolactone) (PCL) films were constructed by incorporation of cellulose derived reduced nano-graphene oxide (r-nGO) carbon nanodots. Two different microwave-assisted reduction routes in superheated water were utilized to obtain r-nGO and r-nGO-CA. For the latter a green reducing agent, caffeic acid (CA) was incorporated in the reduction process. The materials were extruded and compression molded to obtain proper dispersion of the carbon nanodots in the polymer matrix. FTIR results revealed favorable interactions between r-nGO-CA and PCL that improved the dispersion of r-nGO-CA...
February 13, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29437373/porous-heat-treated-polyacrylonitrile-scaffolds-for-bone-tissue-engineering
#16
Miroslav Vetrík, Martin Parizek, Daniel Hadraba, Olivia Kukackova, Jiří Brus, Helena Hlídková, Lucie Komankova, Jiri Hodan, Ondrej Sedlacek, Miroslav Slouf, Lucie Bacakova, Martin Hruby
Heat-treated polyacrylonitrile (HT-PAN), also referred to as Black Orlon (BO), is a promising carbon-based material used for applications in tissue engineering and regenerative medicine. To the best of our knowledge, no such complex bone morphology-mimicking three-dimensional (3D) BO structure has been reported to date. We report that BO can be easily made into 3D cryogel scaffolds with porous structures, using succinonitrile as a porogen. The cryogels possess a porous morphology, similar to bone tissue. The prepared scaffolds showed strong osteoconductive activity, providing excellent support for the adhesion, proliferation and mitochondrial activity of human bone-derived cells...
February 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29436646/in-vitro-evaluation-of-a-bone-morphogenetic-protein%C3%A2-2-nanometer-hydroxyapatite-collagen-scaffold-for-bone-regeneration
#17
Yue Cai, Shuang Tong, Ran Zhang, Tong Zhu, Xukai Wang
Scaffold fabrication and biocompatibility are crucial for successful bone tissue engineering. Nanometer hydroxyapatite (nHAP) combined with collagen (COL) is frequently utilized as a suitable osseous scaffold material. Furthermore, growth factors, including bone morphogenetic protein‑2 (BMP‑2), are used to enhance the scaffold properties. The present study used blending and freeze‑drying methods to develop a BMP‑2‑nHAP‑COL scaffold. An ELISA was performed to determine the BMP‑2 release rate from the scaffold...
February 8, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29436622/hepatocyte-growth-factor-improves-bone-regeneration-via-the-bone-morphogenetic-protein%C3%A2-2%C3%A2-mediated-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway
#18
Ruixin Zhen, Jianing Yang, Yu Wang, Yubo Li, Bin Chen, Youxin Song, Guiyun Ma, Bo Yang
Bone regeneration is an important process associated with the treatment of osteonecrosis, which is caused by various factors. Hepatocyte growth factor (HGF) is an active biological factor that has multifunctional roles in cell biology, life sciences and clinical medicine. It has previously been suggested that bone morphogenetic protein (BMP)‑2 exerts beneficial roles in bone formation, repair and angiogenesis in the femoral head. The present study aimed to investigate the benefits and molecular mechanisms of HGF in bone regeneration...
February 6, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29433862/three-dimensionally-printed-bioactive-ceramic-scaffold-osseoconduction-across-critical-sized-mandibular-defects
#19
Christopher D Lopez, J Rodrigo Diaz-Siso, Lukasz Witek, Jonathan M Bekisz, Bruce N Cronstein, Andrea Torroni, Roberto L Flores, Eduardo D Rodriguez, Paulo G Coelho
BACKGROUND: Vascularized bone tissue transfer, commonly used to reconstruct large mandibular defects, is challenged by long operative times, extended hospital stay, donor-site morbidity, and resulting health care. 3D-printed osseoconductive tissue-engineered scaffolds may provide an alternative solution for reconstruction of significant mandibular defects. This pilot study presents a novel 3D-printed bioactive ceramic scaffold with osseoconductive properties to treat segmental mandibular defects in a rabbit model...
March 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29432987/3d-printed-microchannel-networks-to-direct-vascularisation-during-endochondral-bone-repair
#20
Andrew C Daly, Pierluca Pitacco, Jessica Nulty, Gráinne M Cunniffe, Daniel J Kelly
Bone tissue engineering strategies that recapitulate the developmental process of endochondral ossification offer a promising route to bone repair. Clinical translation of such endochondral tissue engineering strategies will require overcoming a number of challenges, including the engineering of large and often anatomically complex cartilage grafts, as well as the persistence of core regions of avascular cartilage following their implantation into large bone defects. Here 3D printing technology is utilized to develop a versatile and scalable approach to guide vascularisation during endochondral bone repair...
February 1, 2018: Biomaterials
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