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https://www.readbyqxmd.com/read/28447991/isolation-and-characterization-of-mesenchymal-stromal-cells-from-human-umbilical-cord-and-fetal-placenta
#1
Naimisha Beeravolu, Christina McKee, Ali Alamri, Sasha Mikhael, Christina Brown, Mick Perez-Cruet, G Rasul Chaudhry
The human umbilical cord (UC) and placenta are non-invasive, primitive and abundant sources of mesenchymal stromal cells (MSCs) that have increasingly gained attention because they do not pose any ethical or moral concerns. Current methods to isolate MSCs from UC yield low amounts of cells with variable proliferation potentials. Since UC is an anatomically-complex organ, differences in MSC properties may be due to the differences in the anatomical regions of their isolation. In this study, we first dissected the cord/placenta samples into three discrete anatomical regions: UC, cord-placenta junction (CPJ), and fetal placenta (FP)...
April 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447733/gene-expression-profile-in-human-induced-pluripotent-stem-cells-chondrogenic-differentiation-in%C3%A2-vitro-part-b
#2
Ewelina Augustyniak, Wiktoria Maria Suchorska, Tomasz Trzeciak, Magdalena Richter
The development of human induced pluripotent stem cells (hiPSCs) is considered a turning point in tissue engineering. However, more data are required to improve understanding of key aspects of the cell differentiation process, including how specific chondrogenic processes affect the gene expression profile of chondrocyte‑like cells and the relative value of cell differentiation markers. The main aims of the present study were as follows: To determine the gene expression profile of chondrogenic‑like cells derived from hiPSCs cultured in mediums conditioned with HC‑402‑05a cells or supplemented with transforming growth factor β3 (TGF‑β3), and to assess the relative utility of the most commonly‑used chondrogenic markers as indicators of cell differentiation...
March 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28447671/the-scaffold-microenvironment-for-stem-cell-based-bone-tissue-engineering
#3
REVIEW
Zhichao Hao, Zhenhua Song, Jun Huang, Keqing Huang, Amanda Panetta, Zhipeng Gu, Jun Wu
Bone tissue engineering uses the principles and methods of engineering and life sciences to study bone structure, function and growth mechanism for the purposes of repairing, maintaining and improving damaged bone tissue. Scaffolds not only provide structural support for stem cells in cell adhesion and proliferation and bone formation, but also serve as a microenvironment for guiding stem cell differentiation and tissue regeneration and for controlling tissue structure. This review presents the research status of the scaffold microenvironment for bone-related stem cells based on bone tissue engineering...
April 27, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28446370/microcarrier-culture-enhances-osteogenic-potential-of-human-periodontal-ligament-stromal-cells
#4
Alina Čebatariūnienė, Akvilė Jarmalavičiūtė, Virginijus Tunaitis, Alina Pūrienė, Algirdas Venalis, Augustas Pivoriūnas
Regeneration of periodontal tissue represents a major challenge to modern tissue engineering, since cell-based therapies require large amounts of periodontal ligament stromal cells (PLSC), which can be obtained only by in vitro expansion. Ideally, the period of the in vitro expansion should be optimized for the generation of large enough numbers of pre-specified progenitor cells ready to contribute to the restoration of periodontal tissues. In the present study, we used a commercially available, three-dimensional culturing platform and alginate microcarrier cell culture system for the propagation of human PLSCs, which were derived using the explant outgrowth method...
March 29, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28445366/biomechanical-integrity-in-craniofacial-surgery-calvarial-reconstruction-in-favorable-and-infected-defects-with-bone-morphogenetic-protein-2
#5
Zoe M MacIsaac, Sarah E Henderson, Sameer Shakir, Sanjay Naran, Darren M Smith, Liliana Camison, James J Cray, Alejandro J Almarza, Gregory M Cooper, Joseph E Losee
BACKGROUND: The limitations of autologous and alloplastic reconstruction for craniofacial bone defects have created a clinical need for viable tissue-engineering strategies. Recombinant human bone morphogenetic protein-2 (rhBMP-2) has shown promise in this setting. The aim of this study was to determine the long-term biomechanical properties of rhBMP-2-mediated calvarial reconstruction. METHODS: Twelve-week-old New Zealand White rabbits underwent subtotal calvarectomy...
May 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28442664/induction-of-neural-crest-cells-from-human-dental-pulp-derived-induced-pluripotent-stem-cells
#6
Eisuke Kawano, Taku Toriumi, Shinya Iguchi, Daigo Suzuki, Shuichi Sato, Masaki Honda
We previously generated induced pluripotent stem (iPS) cells from human dental pulp cells of deciduous teeth. Neural crest cells (NCCs) play a vital role in the development of the oral and maxillofacial region. Therefore, NCCs represent a cell source for bone, cartilage, and tooth-related tissue engineering. In this study, we examined whether iPS cells are capable of differentiating into NCCs through modification of the human embryonic stem cell protocol. First, iPS cells were dissociated into single cells and then reaggregated in low-cell-adhesion plates with neural induction medium for 8 days in suspension culture to form neurospheres...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28441344/collagenous-extracellular-matrix-biomaterials-for-tissue-engineering-lessons-from-the-common-sea-urchin-tissue
#7
REVIEW
Kheng Lim Goh, David F Holmes
Scaffolds for tissue engineering application may be made from a collagenous extracellular matrix (ECM) of connective tissues because the ECM can mimic the functions of the target tissue. The primary sources of collagenous ECM material are calf skin and bone. However, these sources are associated with the risk of having bovine spongiform encephalopathy or transmissible spongiform encephalopathy. Alternative sources for collagenous ECM materials may be derived from livestock, e.g., pigs, and from marine animals, e...
April 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28437178/a-novel-injectable-recombinant-collagen-i-peptide-based-macroporous-microcarrier-allows-superior-expansion-of-c2c12-and-human-bone-marrow-derived-mesenchymal-stromal-cells-and-supports-deposition-of-mineralized-matrix
#8
Davide Confalonieri, Margherita La Marca, Elisabeth Marianna Wilhelmina Maria van Dongen, Heike Walles, Franziska Ehlicke
The development of scaffold formulations based on extracellular matrix-inspired synthetic materials constitutes an important resource for the advance of cell-based therapies in bone tissue engineering approaches, where both cell and scaffold implantation are often needed. Culturing cells on porous microcarriers (MCs) allows cell expansion in a three-dimensional microenvironment and constitutes a possible solution for minimally invasive cell and scaffold simultaneous delivery, but the reduced pore dimension and pore interconnection diameter of several commercially available microcarriers limits de facto cell ingrowth, and ultimately their suitability for in vivo cell delivery...
March 3, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28435849/effects-of-cyclic-compression-on-the-mechanical-properties-and-calcification-process-of-immature-chick-bone-tissue-in-culture
#9
Eijiro Maeda, Masashi Nakagaki, Katsuhisa Ichikawa, Kazuaki Nagayama, Takeo Matsumoto
Contribution of mechanical loading to tissue growth during both the development and post-natal maturation is of a particular interest, as its understanding would be important to strategies in bone tissue engineering and regenerative medicine. The present study has been performed to investigate how immature bone responds to mechanical loading using an ex vivo culture system. A slice of the tibia, with the thickness of 3 mm, was obtained from 0-day-old chick. For the ex vivo culture experiment in conjunction with cyclic compressive loading, we developed a custom-made, bioreactor system where both the load and the deformation applied to the specimen was recorded...
June 2017: Bone Reports
https://www.readbyqxmd.com/read/28435449/zero-order-controlled-release-of-bmp2-derived-peptide-p24-from-the-chitosan-scaffold-by-chemical-grafting-modification-technique-for-promotion-of-osteogenesis-in-vitro-and-enhancement-of-bone-repair-in-vivo
#10
Yan Chen, Xujie Liu, Rui Liu, Yong Gong, Mingbo Wang, Qianli Huang, Qingling Feng, Bo Yu
Combination of tissue-engineered bone scaffolds with cell-adhesive, osteoconductive, or osteoinductive biomolecules is a critical strategy to improve their properties that significantly influence cellular behaviors, such as adhesion, proliferation, and differentiation, which is beneficial for critical-sized bone defects repairing. However, the traditional surface modification techniques, such as physical adsorption, coating, and plasma treatment, et al, have great limitations for immobilization of bioactive molecules due to undesirable controlled delivery performance or overly complex multistep procedures...
2017: Theranostics
https://www.readbyqxmd.com/read/28435251/comparative-study-of-porous-hydroxyapatite-chitosan-and-whitlockite-chitosan-scaffolds-for-bone-regeneration-in-calvarial-defects
#11
Ding Zhou, Chao Qi, Yi-Xuan Chen, Ying-Jie Zhu, Tuan-Wei Sun, Feng Chen, Chang-Qing Zhang
Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth. In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method. Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28433178/preparation-and-characterization-of-biodegradable-nanocomposites-derived-from-carboxymethyl-cellulose-and-hydroxyapatite
#12
K Azzaoui, E Mejdoubi, A Lamhamdi, S Jodeh, O Hamed, M Berrabah, S Jerdioui, R Salghi, N Akartasse, A Errich, Á Ríos, M Zougagh
Development of a cost-effective technology for extraction and quantification of carcinogenic and toxic organic materials which are widely used in the industry are critical to humans. Membrane technology received much attention and has already been widely used in this area. In this work, we offer a newly developed bio-based nanocomposite membrane for removal of bisphenol-A (BPA) from water. Three natural components hydroxyapatite (HAp), carboxymethyl cellulose and lysine as a diluent were used for making the bio-based membrane...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28433176/novel-hydroxyethyl-chitosan-cellulose-scaffolds-with-bubble-like-porous-structure-for-bone-tissue-engineering
#13
Yaping Wang, Junmin Qian, Na Zhao, Ting Liu, Weijun Xu, Aili Suo
Hydrogels fabricated from natural polysaccharides may serve as ideal scaffolds for tissue engineering because of their similarity to the extracellular matrices. In this study, novel hydrogel scaffolds with bubble-like porous structure were prepared from hydroxyethyl chitosan (HECS) and cellulose (CEL) by a combination of chemical crosslinking, particle-leaching using silicon dioxide particles as porogen and freeze-drying method. The morphology, compression stress-strain curves, wettability, and swelling and rheological behaviors of the HECS/CEL scaffolds were characterized by SEM, mechanical test, contact angle measurement and rheometer...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28432956/introduction-of-n-cadherin-binding-motif-to-alginate-hydrogels-for-controlled-stem-cell-differentiation
#14
Jae Won Lee, Hyoseok An, Kuen Yong Lee
Control of stem cell fate and phenotype using biomimetic synthetic extracellular matrices (ECMs) is an important tissue engineering approach. Many studies have focused on improving cell-matrix interactions. However, proper control of cell-cell interactions using synthetic ECMs could be critical for tissue engineering, especially with undifferentiated stem cells. In this study, alginate hydrogels were modified with a peptide derived from the low-density lipoprotein receptor-related protein 5 (LRP5), which is known to bind to N-cadherin, as a cell-cell interaction motif...
April 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28432001/in-vivo-and-in-vitro-study-of-osteogenic-potency-of-endothelin-1-on-bone-marrow-derived-mesenchymal-stem-cells
#15
Long-Wei Hu, Xiao Wang, Xin-Qun Jiang, Li-Qun Xu, Hong-Ya Pan
Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration. Endothelin-1(EDN-1)is produced by vascular endothelial cells which plays an important role during bone development. However, its role in BMSCs remains largely unknown. We established EDN-1 overexpressed BMSCs, proliferation ability and osteogenesis differentiation were detected respectively...
April 18, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28430676/a-biomimetic-alternative-to-synthetic-hydroxyapatite-boron-containing-bone-like-hydroxyapatite-precipitated-from-simulated-body-fluid
#16
Mert Calis, Tugrul Tolga Demirtas, Alper Vatansever, Gulseren Irmak, Ahmet Hamdi Sakarya, Pergin Atilla, Figen Ozgur, Menemse Gumusderelioglu
BACKGROUND: Biological hydroxyapatite (HA), has several mechanical and physical advantages over the commercially available synthetic apatite (CAP-HA). The aim of this in vivo study was to investigate the effect of osteoinductive "bone-like hydroxyapatite" obtained from simulated body fluid (SBF) combined with osteoinductive "boron" (B) on bone healing. MATERIALS: Bone like nanohydroxyapatite (SBF-HA) was precipitated from 10× simulated body fluid (10×SBF). Thirty Sprague-Dawley rats were randomly divided into 5 experimental groups (n = 6 each)...
April 20, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28429412/bone-extracellular-matrix-hydrogel-enhances-osteogenic-differentiation-of-c2c12-myoblasts-and-mouse-primary-calvarial-cells
#17
Noura Alom, Heather Peto, Glen R Kirkham, Kevin M Shakesheff, Lisa J White
Hydrogel scaffolds derived from the extracellular matrix (ECM) of mammalian tissues have been successfully used to promote tissue repair in vitro and in vivo. The objective of this study was to evaluate the osteogenic potential of ECM hydrogels prepared from demineralized and decellularized bovine bone in the presence and absence of osteogenic medium. Culture of C2C12 and mouse primary calvarial cells (mPCs) on decellularized bone ECM (bECM) and demineralized bone matrix (DBM) gels resulted in increased expression of osteogenic gene markers, including a 3...
April 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28425918/rhein-and-pdms-functionalized-c-c-composites-as-prosthetic-implants-for-bone-repair-application
#18
Zhenzhen Jia, Cui Yang, Jiajia Jiao, Xinyu Li, Dashuai Zhu, Yongchao Yang, Jingyi Yang, Yongzhe Che, Yun Lu, Xizeng Feng
A major issue in bone tissue engineering is the selection of biocompatible materials for implants, to reduce unwanted inflammatory reactions and promote cell adhesion. Bone tissues growth on suitable biomedical implants can shorten recovery and hospitalization after surgery. Therefore, a method to improve tissue-implant integration and healing would be of scientific and clinical interest. In this work, we permeated Polydimethylsiloxane (PDMS) into Carbon/carbon (C/C) composites (PDMS-C/C) and then coated it with 4, 5-dihydroxyanthraquinone-2-carboxylic acid (Rhein) to create Rhein-PDMS-C/C to increase its biocompatibility and reduce the occurrence of inflammatory reactions...
April 20, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28425470/graphene-oxide-as-an-interface-phase-between-polyetheretherketone-and-hydroxyapatite-for-tissue-engineering-scaffolds
#19
Shuping Peng, Pei Feng, Ping Wu, Wei Huang, Youwen Yang, Wang Guo, Chengde Gao, Cijun Shuai
The poor bonding strength between biopolymer and bioceramic has remained an unsolved issue. In this study, graphene oxide (GO) was introduced as an interface phase to improve the interfacial bonding between polyetheretherketone (PEEK) and hydroxyapatite (HAP) for tissue engineering scaffolds. On the one hand, the conjugated structure of GO could form strong π-π stacking interaction with the benzene rings in PEEK. On the other hand, GO with a negatively charge resulting from oxygen functional groups could adsorb the positively charged calcium atoms (C sites) of HAP...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28423500/the-role-of-hesperetin-on-osteogenesis-of-human-mesenchymal-stem-cells-and-its-function-in-bone-regeneration
#20
Deting Xue, Erman Chen, Wei Zhang, Xiang Gao, Shengdong Wang, Qiang Zheng, Zhijun Pan, Hang Li, Ling Liu
Hesperetin has been suggested to be involved in bone strength. We aimed to investigate the effects of hesperetin on the osteogenic differentiation of human mesenchymal stem cells and its related mechanisms. We showed that hesperetin promoted osteogenic differentiation of human mesenchymal stem cells in vitro. It potentially exerts its effects via the ERK and Smad signaling pathways. Using a rat osteotomy model, we showed that human mesenchymal stem cells combined with a hesperetin/gelatin sponge scaffold resulted in accelerated fracture healing in vivo...
March 28, 2017: Oncotarget
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