keyword
MENU ▼
Read by QxMD icon Read
search

bone tissue engineering

keyword
https://www.readbyqxmd.com/read/28230059/synergistic-effects-of-dimethyloxallyl-glycine-and-recombinant-human-bone-morphogenetic-protein-2-on-repair-of-critical-sized-bone-defects-in-rats
#1
Xin Qi, Yang Liu, Zhen-Yu Ding, Jia-Qing Cao, Jing-Huan Huang, Jie-Yuan Zhang, Wei-Tao Jia, Jing Wang, Chang-Sheng Liu, Xiao-Lin Li
In bone remodeling, osteogenesis is closely coupled to angiogenesis. Bone tissue engineering using multifunctional bioactive materials is a promising technique which has the ability to simultaneously stimulate osteogenesis and angiogenesis for repair of bone defects. We developed mesoporous bioactive glass (MBG)-doped poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) composite scaffolds as delivery vehicle. Two bioactive molecules, dimethyloxalylglycine (DMOG), a small-molecule angiogenic drug, and recombinant human bone morphogenetic protein-2 (rhBMP-2), an osteoinductive growth factor, were co-incorporated into the scaffold...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229424/three-dimensional-printed-scaffolds-with-multipotent-mesenchymal-stromal-cells-for-rabbit-mandibular-reconstruction-and-engineering
#2
Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, Simon D Tran
Multipotent mesenchymal stromal cells (MSC) derived from both the bone marrow and adipose tissue possess the ability to differentiate into multiple cell lineages, regulate the immune function by secreting numerous bioactive paracrine factors, and hold great potential in cell therapy and tissue engineering. When combined with three-dimensional (3D) scaffolds, MSC can be used for bone defect reconstruction and engineering. This protocol describes the isolation of bone marrow mesenchymal stromal cells (BMMSC) and adipose-tissue derived stem cells (ADSC) from rabbits for subsequent seeding on tissue-engineered 3D-printed scaffolds and transplantation into a rabbit-model with the goal of repairing large osseous mandibular defects (one quarter of the lower jaw is removed surgically)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28227444/novel-3d-coaxial-flow-focusing-nozzle-device-for-the-production-of-monodispersed-collagen-microspheres
#3
Sravani Jaligama, Po-Jung Huang, Jun Kameoka, Sravani Jaligama, Po-Jung Huang, Jun Kameoka, Jun Kameoka, Sravani Jaligama, Po-Jung Huang
We have developed a 3D coaxial flow-focusing nozzle device for the mass production of monodispersed collagen microspheres and chemically crosslinked them using EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide) and N-hydroxysuccinimide (NHS). The size of the microspheres was varied between 200 μm and 600 μm by adjusting the ratio of the flow rates of the dispersed and continuous phases. MDA231-GFP cells were attached to the surface of these particles and their viability was investigated. Because they are comprised of a natural biomaterial, these collagen microspheres will have numerous applications, including bone regeneration scaffolds for tissue engineering and analyses of cancer cell interactions in a 3D environment...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225583/microfluidic-encapsulation-of-human-mesenchymal-stem-cells-for-articular-cartilage-tissue-regeneration
#4
Fanyi Li, Vinh X Truong, Helmut Thissen, Jessica Frith, John S Forsythe
Stem cell injections for the treatment of articular cartilage damage are a promising approach to achieve tissue regeneration. However, this method is encumbered by high cell apoptosis rates, low retention in the cartilage lesion and inefficient chondrogenesis. Here, we have used a facile, very low cost-based microfluidic technique to create visible light-cured hydrogel microparticles composed of gelatin norbornene (GelNB) and a polyethylene glycol (PEG) crosslinker. In addition, we have demonstrated that the process enables the rapid in situ micro-encapsulation of human bone marrow-derived mesenchymal stem cells (hBMSCs) under biocompatible microfluidic-processing conditions for long-term maintenance...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28222206/titanium-hydroxyapatite-composites-sintered-at-low-temperature-for-tissue-engineering-in-vitro-cell-support-and-biocompatibility
#5
Romina Comín, Mariana P Cid, Luciano Grinschpun, Carlos Oldani, Nancy A Salvatierra
BACKGROUND: In clinical orthopedics, a critical problem is the bone tissue loss produced by a disease or injury. The use of composites from titanium and hydroxyapatite for biomedical applications has increased due to the resulting advantageous combination of hydroxyapatite bioactivity and favorable mechanical properties of titanium. Powder metallurgy is a simple and lower-cost method that uses powder from titanium and hydroxyapatite to obtain composites having hydroxyapatite phases in a metallic matrix...
February 21, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28222169/decellularized-wharton-s-jelly-from-human-umbilical-cord-as-a-novel-3d-scaffolding-material-for-tissue-engineering-applications
#6
Sushma Jadalannagari, Gabriel Converse, Christopher McFall, Eric Buse, Michael Filla, Maria T Villar, Antonio Artigues, Adam J Mellot, Jinxi Wang, Michael S Detamore, Richard A Hopkins, Omar S Aljitawi
In tissue engineering, an ideal scaffold attracts and supports cells thus providing them with the necessary mechanical support and architecture as they reconstruct new tissue in vitro and in vivo. This manuscript details a novel matrix derived from decellularized Wharton's jelly (WJ) obtained from human umbilical cord for use as a scaffold for tissue engineering application. This decellularized Wharton's jelly matrix (DWJM) contained 0.66 ± 0.12 μg/mg sulfated glycosaminoglycans (GAGs), and was abundant in hyaluronic acid, and completely devoid of cells...
2017: PloS One
https://www.readbyqxmd.com/read/28222045/validation-of-scaffold-design-optimization-in-bone-tissue-engineering-finite-element-modeling-versus-designed-experiments
#7
Nicholas Uth, Jens Mueller, Byran Smucker, Azizeh-Mitra Yousefi
This study reports the development of biological/synthetic scaffolds for bone tissue engineering (TE) via 3D bioplotting. These scaffolds were composed of poly(L-lactic-co-glycolic acid) (PLGA), type I collagen, and nano-hydroxyapatite (nHA) in an attempt to mimic the extracellular matrix of bone. The solvent used for processing the scaffolds was 1,1,1,3,3,3-hexafluoro-2-propanol. The produced scaffolds were characterized by scanning electron microscopy, microcomputed tomography, thermogravimetric analysis, and unconfined compression test...
February 21, 2017: Biofabrication
https://www.readbyqxmd.com/read/28217782/receptor-for-hyaluronan-mediated-motility-rhamm-hmmr-is-a-novel-target-for-promoting-subcutaneous-adipogenesis
#8
S B Bahrami, C Tolg, T Peart, C Symonette, M Veiseh, J U Umoh, D W Holdsworth, J B McCarthy, L G Luyt, M J Bissell, A Yazdani, E A Turley
Hyaluronan, CD44 and the Receptor for Hyaluronan-Mediated Motility (RHAMM, gene name HMMR) regulate stem cell differentiation including mesenchymal progenitor differentiation. Here, we show that CD44 expression is required for subcutaneous adipogenesis, whereas RHAMM expression suppresses this process. We designed RHAMM function blocking peptides to promote subcutaneous adipogenesis as a clinical and tissue engineering tool. Adipogenic RHAMM peptides were identified by screening for their ability to promote adipogenesis in culture assays using rat bone marrow mesenchymal stem cells, mouse pre-adipocyte cell lines and primary human subcutaneous pre-adipocytes...
February 20, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28217659/the-prospective-opportunities-offered-by-magnetic-scaffolds-for-bone-tissue-engineering-a-review
#9
Alessandro Ortolani, Michele Bianchi, Massimiliano Mosca, Silvio Caravelli, Mario Fuiano, Maurilio Marcacci, Alessandro Russo
Magnetic scaffolds are becoming increasingly attractive in tissue engineering, due to their ability to enhance bone tissue formation by attracting soluble factors, such as growth factors, hormones and polypeptides, directly to the implantation site, as well as their potential to improve the fixation and stability of the implant. Moreover, there is increasing evidence that the synergistic effects of magnetic scaffolds and magnetic fields can promote bone repair and regeneration. In this manuscript we review the recent innovations in bone tissue engineering that exploit magnetic biomaterials combined with static magnetic fields to enhance bone cell adhesion and proliferation, and thus bone tissue growth...
October 2016: Joints
https://www.readbyqxmd.com/read/28213098/gelatin-based-3d-conduits-for-transdifferentiation-of-mesenchymal-stem-cells-into-schwann-cell-like-phenotypes
#10
Metin Uz, Melda Buyukoz, Anup D Sharma, Donald S Sakaguchi, Sacide Alsoy Altinkaya, Surya K Mallapragada
: In this study, gelatin-based 3D conduits with three different microstructures (nanofibrous, macroporous and ladder-like) were fabricated for the first time via combined molding and thermally induced phase separation (TIPS) technique for peripheral nerve regeneration. The effects of conduit microstructure and mechanical properties on the transdifferentiation of bone marrow-derived mesenchymal stem cells (MSCs) into Schwann cell (SC) like phenotypes were examined to help facilitate neuroregeneration and understand material-cell interfaces...
February 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28213096/ectopic-tissue-engineered-ligament-with-silk-collagen-scaffold-for-acl-regeneration-a-preliminary-study
#11
Jisheng Ran, Yejun Hu, Huihui Le, Yangwu Chen, Zefeng Zheng, Xiao Chen, Zi Yin, Ruijian Yan, Zhangchu Jin, Chenqi Tang, Jiayun Huang, Yanjia Gu, Langhai Xu, Shengjun Qian, Wei Zhang, Boon Chin Heng, Pioletti Dominique, Weishan Chen, Lidong Wu, Weiliang Shen, Hongwei Ouyang
: Anterior cruciate ligament (ACL) reconstruction remains a formidable clinical challenge because of the lack of vascularization and adequate cell numbers in the joint cavity. In this study, we developed a novel strategy to mimic the early stage of repair in vivo, which recapitulated extra-articular inflammatory response to facilitate the early ingrowth of blood vessels and cells. A vascularized ectopic tissue engineered ligament (ETEL) with silk collagen scaffold was developed and then transferred to reconstruct the ACL in rabbits without interruption of perfusion...
February 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28211911/three-dimensional-printing-of-calcium-sulfate-and-mesoporous-bioactive-glass-scaffolds-for-improving-bone-regeneration-in-vitro-and-in-vivo
#12
Xin Qi, Peng Pei, Min Zhu, Xiaoyu Du, Chen Xin, Shichang Zhao, Xiaolin Li, Yufang Zhu
In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28211493/25-hydroxyvitamin-d3-induces-osteogenic-differentiation-of-human-mesenchymal-stem-cells
#13
Yan-Ru Lou, Tai Chong Toh, Yee Han Tee, Hanry Yu
25-Hydroxyvitamin D3 [25(OH)D3] has recently been found to be an active hormone. Its biological actions are demonstrated in various cell types. 25(OH)D3 deficiency results in failure in bone formation and skeletal deformation. Here, we investigated the effect of 25(OH)D3 on osteogenic differentiation of human mesenchymal stem cells (hMSCs). We also studied the effect of 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2D3], a metabolite of 25(OH)D3. One of the vitamin D responsive genes, 25(OH)D3-24-hydroxylase (cytochrome P450 family 24 subfamily A member 1) mRNA expression is up-regulated by 25(OH)D3 at 250-500 nM and by 1α,25-(OH)2D3 at 1-10 nM...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210629/cellular-mechanisms-of-liver-regeneration-and-cell-based-therapies-of-liver-diseases
#14
REVIEW
Irina V Kholodenko, Konstantin N Yarygin
The emerging field of regenerative medicine offers innovative methods of cell therapy and tissue/organ engineering as a novel approach to liver disease treatment. The ultimate scientific foundation of both cell therapy of liver diseases and liver tissue and organ engineering is delivered by the in-depth studies of the cellular and molecular mechanisms of liver regeneration. The cellular mechanisms of the homeostatic and injury-induced liver regeneration are unique. Restoration of the mass of liver parenchyma is achieved by compensatory hypertrophy and hyperplasia of the differentiated parenchymal cells, hepatocytes, while expansion and differentiation of the resident stem/progenitor cells play a minor or negligible role...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28210626/osteogenic-differentiation-capacity-of-in-vitro-cultured-human-skeletal-muscle-for-expedited-bone-tissue-engineering
#15
Chunlei Miao, Lulu Zhou, Lufeng Tian, Yingjie Zhang, Wei Zhang, Fanghong Yang, Tianyi Liu, Shengjian Tang, Fangjun Liu
Expedited bone tissue engineering employs the biological stimuli to harness the intrinsic regenerative potential of skeletal muscle to trigger the reparative process in situ to improve or replace biological functions. When genetically modified with adenovirus mediated BMP2 gene transfer, muscle biopsies from animals have demonstrated success in regenerating bone within rat bony defects. However, it is uncertain whether the human adult skeletal muscle displays an osteogenic potential in vitro when a suitable biological trigger is applied...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28210623/in-vitro-and-in-vivo-evaluation-of-commercially-available-fibrin-gel-as-a-carrier-of-alendronate-for-bone-tissue-engineering
#16
Beom Su Kim, Feride Shkembi, Jun Lee
Alendronate (ALN) is a bisphosphonate drug that is widely used for the treatment of osteoporosis. Furthermore, local delivery of ALN has the potential to improve the bone regeneration. This study was designed to investigate an ALN-containing fibrin (fibrin/ALN) gel and evaluate the effect of this gel on both in vitro cellular behavior using human mesenchymal stem cells (hMSCs) and in vivo bone regenerative capacity. Fibrin hydrogels were fabricated using various ALN concentrations (10(-7)-10(-4 )M) with fibrin glue and the morphology, mechanical properties, and ALN release kinetics were characterized...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28209631/hdpscs-fabricate-a-vascularised-woven-bone-tissue-a-new-tool-in-bone-tissue-engineering
#17
Francesca Paino, Marcella La Noce, Alessandra Giuliani, Alfredo De Rosa, Serena Mazzoni, Luigi Laino, Evzen Amler, Gianpaolo Papaccio, Vincenzo Desiderio, Virginia Tirino
Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells successfully used in human bone tissue engineering. To better establish if these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim to obtain a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal-vascular dental pulp fraction, using appropriate markers and cultured. Growth curves, expression of bone-related markers, calcification, angiogenesis as well as in vivo transplantation assay were performed...
February 16, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28209379/endocultivation-continuous-application-of-rhbmp-2-via-mini-osmotic-pumps-to-induce-bone-formation-at-extraskeletal-sites
#18
B E Beck-Broichsitter, S T Becker, H Seitz, J Wiltfang, P H Warnke
The continuous presence of recombinant human bone morphogenetic protein 2 (rhBMP-2) inside a scaffold may be crucial to the outcome in bone tissue engineering. This study investigated whether the release of the growth factor rhBMP-2 via different continuous application schemes influences histomorphological aspects of the hard and soft tissues induced. Three-dimensionally printed hydroxyapatite scaffolds were implanted into one latissimus dorsi muscle of 42 female Lewis rats. Simultaneously implanted mini-osmotic pumps were used to provide a continuous application of rhBMP-2 over 1, 2, or 4 weeks (total dose 200μg)...
February 10, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28208578/role-of-cortico-cancellous-heterologous-bone-in-human-periodontal-ligament-stem-cell-xeno-free-culture-studied-by-synchrotron-radiation-phase-contrast-microtomography
#19
Serena Mazzoni, Sara Mohammadi, Giuliana Tromba, Francesca Diomede, Adriano Piattelli, Oriana Trubiani, Alessandra Giuliani
This study was designed to quantitatively demonstrate via three-dimensional (3D) images, through the Synchrotron Radiation Phase-Contrast Microtomography (SR-PhC-MicroCT), the osteoinductive properties of a cortico-cancellous scaffold (Osteobiol Dual Block-DB) cultured with human Periodontal Ligament Stem Cells (hPDLSCs) in xeno-free media. In vitro cultures of hPDLSCs, obtained from alveolar crest and horizontal fibers of the periodontal ligament, were seeded onto DB scaffolds and cultured in xeno-free media for three weeks...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28204808/bone-morphogenetic-protein-2-enhances-the-osteogenic-differentiation-capacity-of-mesenchymal-stromal-cells-derived-from-human-bone-marrow-and-umbilical-cord
#20
Kulisara Marupanthorn, Chairat Tantrawatpan, Pakpoom Kheolamai, Duangrat Tantikanlayaporn, Sirikul Manochantr
Mesenchymal stromal cells (MSCs) are multipotent cells that can give rise to different cell types of the mesodermal lineages. They are powerful sources for cell therapy in regenerative medicine as they can be isolated from various tissues, and can be expanded and induced to differentiate into multiple lineages. Recently, the umbilical cord has been suggested as an alternative source of MSCs. Although MSCs derived from the umbilical cord can be induced to differentiate into osteoblasts with a phenotypic similarity to that of bone marrow-derived MSCs, the differentiation ability is not consistent...
March 2017: International Journal of Molecular Medicine
keyword
keyword
53355
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"