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https://www.readbyqxmd.com/read/29147064/lncrna-neat1-regulates-cell-viability-and-invasion-in-esophageal-squamous-cell-carcinoma-through-the-mir-129-ctbp2-axis
#1
Yong Li, Dong Chen, Xiang Gao, Xiaohui Li, Gongning Shi
Background: Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) was reported to be aberrantly upregulated and promote esophageal squamous cell carcinoma (ESCC) cell progression. Nevertheless, the molecular mechanism of NEAT1 involved in the competing endogenous RNA (ceRNA) regulatory network in ESCC progression remains poorly defined. Methods: The expressions of NEAT1, miR-129, and C-terminal-binding protein 2 (CTBP2) in ESCC cells were examined by qRT-PCR...
2017: Disease Markers
https://www.readbyqxmd.com/read/29113206/nuclear-paraspeckle-assembly-transcript-1-promotes-the-metastasis-and-epithelial-mesenchymal-transition-of-hepatoblastoma-cells-by-inhibiting-mir-129-5p
#2
Ming-Cui Fu, Li-Qun Yuan, Ting Zhang, Xiang-Ming Yan, Yun Zhou, Hong-Liang Xia, Yi Wu, Li-Xiao Xu, Xu Cao, Jian Wang
The abnormal expression of nuclear paraspeckle assembly transcript 1 (NEAT1) may serve critical functions for the development and progression of various types of human tumor. However, the expression and biological function of NEAT1 in hepatoblastoma (HB) and the underlying mechanisms for the function of NEAT1 in HB remain largely uncharacterized. In the present study, the results of reverse transcription-quantitative polymerase chain reaction revealed that the expression of NEAT1 was significantly elevated in HB tissues...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29075020/the-rbm14-coaa-interacting-long-intergenic-non-coding-rna-paral1-regulates-adipogenesis-and-coactivates-the-nuclear-receptor-ppar%C3%AE
#3
François F Firmin, Frederik Oger, Céline Gheeraert, Julie Dubois-Chevalier, Anne-Sophie Vercoutter-Edouart, Fawaz Alzaid, Claire Mazuy, Hélène Dehondt, Jeremy Alexandre, Bruno Derudas, Quentin Dhalluin, Maheul Ploton, Alexandre Berthier, Eloise Woitrain, Tony Lefebvre, Nicolas Venteclef, François Pattou, Bart Staels, Jérôme Eeckhoute, Philippe Lefebvre
Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there is a need for a thorough understanding of the transcriptional regulatory network involved in adipose tissue pathophysiology. Recent advances in the functional annotation of the genome has highlighted the role of non-coding RNAs in cellular differentiation processes in coordination with transcription factors...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29069457/nol12-is-a-multifunctional-rna-binding-protein-at-the-nexus-of-rna-and-dna-metabolism
#4
Daniel D Scott, Christian Trahan, Pierre J Zindy, Lisbeth C Aguilar, Marc Y Delubac, Eric L Van Nostrand, Srivathsan Adivarahan, Karen E Wei, Gene W Yeo, Daniel Zenklusen, Marlene Oeffinger
To counteract the breakdown of genome integrity, eukaryotic cells have developed a network of surveillance pathways to prevent and resolve DNA damage. Recent data has recognized the importance of RNA binding proteins (RBPs) in DNA damage repair (DDR) pathways. Here, we describe Nol12 as a multifunctional RBP with roles in RNA metabolism and genome maintenance. Nol12 is found in different subcellular compartments-nucleoli, where it associates with ribosomal RNA and is required for efficient separation of large and small subunit precursors at site 2; the nucleoplasm, where it co-localizes with the RNA/DNA helicase Dhx9 and paraspeckles; as well as GW/P-bodies in the cytoplasm...
October 23, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29047232/knockdown-of-long-non-coding-rna-neat1-inhibits-proliferation-and-invasion-and-induces-apoptosis-of-osteosarcoma-by-inhibiting-mir-194-expression
#5
Heping Wang, Yanzhang Yu, Shuxin Fan, Leifeng Luo
PURPOSE: Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated as an oncogene in the development and progression of osteosarcoma. This study aims to explore the mechanism of NEAT1 in osteosarcoma. MATERIALS AND METHODS: Expressions of NEAT1 and miR-194 in osteosarcoma tissues and cells were detected by quantitative real-time PCR. The effects of NEAT1 knockdown or miR-194 overexpression on cell proliferation, invasion, and apoptosis were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay, transwell invasive assay, and flow cytometry analysis, respectively...
November 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/29033980/cellular-physiological-and-pathological-aspects-of-the-long-non-coding-rna-neat1
#6
Pang-Kuo Lo, Benjamin Wolfson, Qun Zhou
BACKGROUND: The majority of mammalian genomes have been found to be transcribed into non-coding RNAs. One category of non-coding RNAs is classified as long non-coding RNAs (lncRNAs) based on their transcript sizes larger than 200 nucleotides. Growing evidence has shown that lncRNAs are not junk transcripts and play regulatory roles in multiple aspects of biological processes. Dysregulation of lncRNA expression has also been linked to diseases, in particular cancer. Therefore, studies of lncRNAs have attracted significant interest in the field of medical research...
December 2016: Frontiers in Biology
https://www.readbyqxmd.com/read/28993096/altered-expression-of-malat1-lncrna-in-nonalcoholic-steatohepatitis-fibrosis-regulates-cxcl5-in-hepatic-stellate-cells
#7
Fatjon Leti, Christophe Legendre, Christopher D Still, Xin Chu, Anthony Petrick, Glenn S Gerhard, Johanna K DiStefano
In the present study, we sought to identify long noncoding RNA (lncRNA) expression profiles in nonalcoholic steatohepatitis (NASH) patients with histologic evidence of lobular inflammation and advanced fibrosis. We profiled lncRNA expression using RNA-sequencing of wedge liver biopsies from 24 nonalcoholic fatty liver disease (NAFLD) patients with normal liver histology, 53 NAFLD patients with lobular inflammation, and 65 NAFLD patients with advanced fibrosis. Transcript profiling identified 4432 and 4057 differentially expressed lncRNAs in comparisons of normal tissue with lobular inflammation and fibrosis samples, respectively...
September 19, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28981074/paraspeckles-nuclear-nests-helping-to-raise-mature-mirnas
#8
Jacek Krol
No abstract text is available yet for this article.
October 5, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28977530/nuclear-poly-a-binding-protein-1-pabpn1-and-matrin3-interact-in-muscle-cells-and-regulate-rna-processing
#9
Ayan Banerjee, Katherine E Vest, Grace K Pavlath, Anita H Corbett
The polyadenylate binding protein 1 (PABPN1) is a ubiquitously expressed RNA binding protein vital for multiple steps in RNA metabolism. Although PABPN1 plays a critical role in the regulation of RNA processing, mutation of the gene encoding this ubiquitously expressed RNA binding protein causes a specific form of muscular dystrophy termed oculopharyngeal muscular dystrophy (OPMD). Despite the tissue-specific pathology that occurs in this disease, only recently have studies of PABPN1 begun to explore the role of this protein in skeletal muscle...
October 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28946559/neat1-negatively-regulates-mir-218-expression-and-promotes-breast-cancer-progression
#10
Dahua Zhao, Ying Zhang, Nana Wang, Ning Yu
BACKGROUND: Studies have shown that lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is related to breast cancer progression, however, the role of NEAT1 remains largely unknown. The aim of the current study was to further investigate the function of NEAT1 involved in breast cancer. METHODS: QRT-PCR was used to analyze lncRNA NEAT1 expression in breast cancer tissues and determine the association between NEAT1 and clinicopathologic features in patients. Kaplan-Meier analysis and the log-rank test were used to establish the relationship between NEAT1 and overall survival...
September 7, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28864835/neat1-accelerates-the-progression-of-liver-fibrosis-via-regulation-of-microrna-122-and-kruppel-like-factor-6
#11
Fujun Yu, Zhe Jiang, Bicheng Chen, Peihong Dong, Jianjian Zheng
Long non-coding RNAs (lncRNAs) have been reported to be involved in many important biological processes including proliferation, apoptosis, differentiation, and survival. Recently, nuclear paraspeckle assembly transcript 1 (NEAT1), a novel lncRNA, serves as a crucial regulator in tumors. However, the biological role of NEAT1 in liver fibrosis is largely unknown. In this study, the role of NEAT1 was explored in primary mouse hepatic stellate cells (HSCs) and carbon tetrachloride (CCl4)-induced mouse liver fibrosis models...
November 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28846091/neat1-scaffolds-rna-binding-proteins-and-the-microprocessor-to-globally-enhance-pri-mirna-processing
#12
Li Jiang, Changwei Shao, Qi-Jia Wu, Geng Chen, Jie Zhou, Bo Yang, Hairi Li, Lan-Tao Gou, Yi Zhang, Yangming Wang, Gene W Yeo, Yu Zhou, Xiang-Dong Fu
MicroRNA (miRNA) biogenesis is known to be modulated by a variety of RNA-binding proteins (RBPs), but in most cases, individual RBPs appear to influence the processing of a small subset of target miRNAs. Here, we report that the RNA-binding NONO-PSF heterodimer binds a large number of expressed pri-miRNAs in HeLa cells to globally enhance pri-miRNA processing by the Drosha-DGCR8 Microprocessor. NONO and PSF are key components of paraspeckles organized by the long noncoding RNA (lncRNA) NEAT1. We further demonstrate that NEAT1 also has a profound effect on global pri-miRNA processing...
October 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28810932/lncrna-neat1-promotes-growth-and-metastasis-of-cholangiocarcinoma-cells
#13
Cheng Zhang, Jing-Yi Li, Fu-Zhou Tian, Gang Zhao, Hai Hu, Yue-Feng Ma, Yu-Long Yang
Long non-coding RNAs (lncRNAs) are known to play important roles in cancers. However, little is known about lncRNAs incholangiocarcinoma (CCA), a cholangiocyte malignancy with poor prognosis. We investigated the role of Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) lncRNA in promoting cholangiocarcinoma (CCA). qRT-PCR analysis of patient samples showed that NEAT1 expression was higher in CCA tumors than matched adjacent non-tumor tissue. NEAT1 levels were also higher in CCA cell lines than a normal biliary epithelium cell line (HIBEpic)...
August 15, 2017: Oncology Research
https://www.readbyqxmd.com/read/28728342/role-of-nuclear-paraspeckle-assembly-transcript-1-as-a-common-molecular-marker-for-prognosis-in-various-cancers
#14
Zhicong Chen, Haibiao Xie, Yucheng Zhong, Yuchen Liu, Weiren Huang
No abstract text is available yet for this article.
October 2017: Minerva Medica
https://www.readbyqxmd.com/read/28712728/hexim1-and-neat1-long-non-coding-rna-form-a-multi-subunit-complex-that-regulates-dna-mediated-innate-immune-response
#15
Mehdi Morchikh, Alexandra Cribier, Raoul Raffel, Sonia Amraoui, Julien Cau, Dany Severac, Emeric Dubois, Olivier Schwartz, Yamina Bennasser, Monsef Benkirane
The DNA-mediated innate immune response underpins anti-microbial defenses and certain autoimmune diseases. Here we used immunoprecipitation, mass spectrometry, and RNA sequencing to identify a ribonuclear complex built around HEXIM1 and the long non-coding RNA NEAT1 that we dubbed the HEXIM1-DNA-PK-paraspeckle components-ribonucleoprotein complex (HDP-RNP). The HDP-RNP contains DNA-PK subunits (DNAPKc, Ku70, and Ku80) and paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATRIN3). We show that binding of HEXIM1 to NEAT1 is required for its assembly...
August 3, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28698299/neat1-is-a-p53-inducible-lincrna-essential-for-transformation-suppression
#16
Stephano S Mello, Carolyn Sinow, Nitin Raj, Pawel K Mazur, Kathryn Bieging-Rolett, Daniela Kenzelmann Broz, Jamie F Conklin Imam, Hannes Vogel, Laura D Wood, Julien Sage, Tetsuro Hirose, Shinichi Nakagawa, John Rinn, Laura D Attardi
The p53 gene is mutated in over half of all cancers, reflecting its critical role as a tumor suppressor. Although p53 is a transcriptional activator that induces myriad target genes, those p53-inducible genes most critical for tumor suppression remain elusive. Here, we leveraged p53 ChIP-seq (chromatin immunoprecipitation [ChIP] combined with high-throughput sequencing) and RNA-seq (RNA sequencing) data sets to identify new p53 target genes, focusing on the noncoding genome. We identify Neat1, a noncoding RNA (ncRNA) constituent of paraspeckles, as a p53 target gene broadly induced by mouse and human p53 in different cell types and by diverse stress signals...
June 1, 2017: Genes & Development
https://www.readbyqxmd.com/read/28673861/cpsf6-is-a-clinically-relevant-breast-cancer-vulnerability-target-role-of-cpsf6-in-breast-cancer
#17
Najat Binothman, Ibrahim Y Hachim, Jean-Jacques Lebrun, Suhad Ali
Breast cancer represents a major health challenge. The majority of breast cancer deaths are due to cancer progression/recurrence for which no efficient therapies exist. Aggressive breast cancers are characterized by loss of cellular differentiation. Defining molecular mechanisms/targets contributing to cancer aggressiveness is needed to guide the design of new screening and targeted treatments. Here, we describe a novel tumor promoting function for the Cleavage and Polyadenylation Factor-6 (CPSF6). Importantly, aggressive breast cancer cells of luminal B, HER2-overexpressing and triple negative subtypes show dependency on CPSF6 for viability and tumorigenic capacity...
July 2017: EBioMedicine
https://www.readbyqxmd.com/read/28615056/oct4-transcriptionally-regulates-the-expression-of-long-non-coding-rnas-neat1-and-malat1-to-promote-lung-cancer-progression
#18
Jayu Jen, Yen-An Tang, Ying-Hung Lu, Che-Chung Lin, Wu-Wei Lai, Yi-Ching Wang
BACKGROUND: Oct4, a key stemness transcription factor, is overexpressed in lung cancer. Here, we reveal a novel transcription regulation of long non-coding RNAs (lncRNAs) by Oct4. LncRNAs have emerged as important players in cancer progression. METHODS: Oct4 chromatin-immunoprecipitation (ChIP)-sequencing and several lncRNA databases with literature annotation were integrated to identify Oct4-regulated lncRNAs. Luciferase activity, qRT-PCR and ChIP-PCR assays were conducted to examine transcription regulation of lncRNAs by Oct4...
June 14, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28604724/visualizing-adenosine-to-inosine-rna-editing-in-single-mammalian-cells
#19
Ian A Mellis, Rohit Gupte, Arjun Raj, Sara H Rouhanifard
Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about the biology of this conversion remain unknown because of a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine-edited transcripts in situ. We found that editing of the GRIA2, EIF2AK2, and NUP43 transcripts is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing levels vary...
August 2017: Nature Methods
https://www.readbyqxmd.com/read/28593374/chromatin-loops-and-causality-loops-the-influence-of-rna-upon-spatial-nuclear-architecture
#20
REVIEW
Iain A Sawyer, Miroslav Dundr
An intrinsic and essential trait exhibited by cells is the properly coordinated and integrated regulation of an astoundingly large number of simultaneous molecular decisions and reactions to maintain biochemical homeostasis. This is especially true inside the cell nucleus, where the recognition of DNA and RNA by a vast range of nucleic acid-interacting proteins organizes gene expression patterns. However, this dynamic system is not regulated by simple "on" or "off" signals. Instead, transcription factor and RNA polymerase recruitment to DNA are influenced by the local chromatin and epigenetic environment, a gene's relative position within the nucleus and the action of noncoding RNAs...
October 2017: Chromosoma
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