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https://www.readbyqxmd.com/read/29312630/neat1-in-hematopoietic-stem-cells
#1
Noam Fallik, Yael Bar-Lavan, Yariv Greenshpan, Oron Goldstein, Markus Grosch, Micha Drukker, Roi Gazit
Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs (lncRNA) in HSCs. Neat1 is a lncRNA that plays a major role in the formation of sub-nuclear structures called paraspeckles, and was reported to regulate proliferation and differentiation in other cells types. We detected Neat1 expression using RNA-seq data and RT-qPCR in HSCs, progenitors and effector immune cells, by specific detection of its isoforms...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29289458/paraspeckles-where-long-noncoding-rna-meets-phase-separation
#2
REVIEW
Archa H Fox, Shinichi Nakagawa, Tetsuro Hirose, Charles S Bond
Long noncoding RNA (lncRNA) molecules are some of the newest and least understood players in gene regulation. Hence, we need good model systems with well-defined RNA and protein components. One such system is paraspeckles - protein-rich nuclear organelles built around a specific lncRNA scaffold. New discoveries show how paraspeckles are formed through multiple RNA-protein and protein-protein interactions, some of which involve extensive polymerization, and others with multivalent interactions driving phase separation...
December 27, 2017: Trends in Biochemical Sciences
https://www.readbyqxmd.com/read/29287722/lncrna-neat1-promotes-autophagy-in-mptp-induced-parkinson-s-disease-through-stabilizing-pink1-protein
#3
Wang Yan, Zhao-Ying Chen, Jia-Qi Chen, Hui-Min Chen
BACKGROUND: Long non-coding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1) was found to be closely related to the pathological changes in brain and nervous system. However, the role of NEAT1 and its potential mechanism in Parkinson's disease (PD) largely remain uncharacterized. METHODS: In this study, PD mouse model was established by intraperitoneal injection of MPTP. The numbers of TH + neurons, NEAT1 expression and the level of PINK1, LC3-II, LC3-I protein were assessed in PD mice...
December 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29222807/long-non-coding-rna-expression-in-bladder-cancer
#4
REVIEW
Mohammad Taheri, Mir Davood Omrani, Soudeh Ghafouri-Fard
The advent of novel high-throughput sequencing methods has facilitated identification of non-coding RNAs with fundamental roles in cellular biological and pathological conditions. A group of these consisting of at least 200 nucleotides are called long non-coding RNAs (lncRNAs). Their participation in the pathogenesis of cancer has been highlighted in recent years. Bladder cancer, one of the most prevalent cancers worldwide, exhibits altered expression levels of several lncRNAs. Several in vitro and in vivo studies have assessed the effects of silencing RNAs on cancer cell phenotypes and in vivo tumor growth...
December 8, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/29207151/long-non%C3%A2-coding-nuclear-paraspeckle-assembly-transcript-1-acts-as-prognosis-biomarker-and-increases-cell-growth-and-invasion-in-cervical-cancer-by-sequestering-microrna%C3%A2-101
#5
Li Wang, Hongying Zhu
Emerging studies have focused on the essential role of long non‑coding RNAs (lncRNAs) in cervical carcinogenesis. A recent study has indicated that nuclear paraspeckle assembly transcript 1 (NEAT1) is highly expressed in the human cervical tissue. However, whether NEAT1 is involved in cervical cancer remains to be elucidated. Reverse transcription‑quantitative polymerase chain reaction results demonstrated that the expression of NEAT1 was higher in cervical cancer cells/tissues compared with that in normal human keratinocytes/tissues...
November 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29205703/lncrna-neat1-promotes-dexamethasone-resistance-in-multiple-myeloma-by-targeting-mir-193a-mcl1-pathway
#6
Yilan Wu, Han Wang
Although dexamethasone (DEX) remains a first-line agent for multiple myeloma (MM) therapy, the development of DEX resistance has become an indicator of poor prognosis in MM patients. It is thus urgent to develop strategies to restore the vulnerability of MM to DEX. This study demonstrated long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) was highly expressed in DEX-resistant myeloma cell lines, and upregulation of NEAT1 was tightly linked to poor prognosis. The in-depth study revealed that during the development of DEX resistance in these cells, the miR-193a levels were decreased, which resulted in the increased expression of the target gene myeloid cell leukemia-1 (MCL1)...
December 4, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/29180871/lncrna-neat1-contributes-to-paclitaxel-resistance-of-ovarian-cancer-cells-by-regulating-zeb1-expression-via-mir-194
#7
Jihong An, Weiling Lv, Yongzhou Zhang
Background: Chemoresistance is one of the major obstacles for cancer therapy in the clinic. Nuclear paraspeckle assembly transcript 1 (NEAT1) has been reported as an oncogene in most malignancies such as lung cancer, esophageal cancer, and gastric cancer. This study is designed to investigate the function of NEAT1 in paclitaxel (PTX) resistance of ovarian cancer and its potential molecular mechanism. Patients and methods: The expressions of NEAT1 and miR-194 in ovarian cancer tissues and cells were estimated by quantitative real-time polymerase chain reaction (qRT-PCR)...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29147064/lncrna-neat1-regulates-cell-viability-and-invasion-in-esophageal-squamous-cell-carcinoma-through-the-mir-129-ctbp2-axis
#8
Yong Li, Dong Chen, Xiang Gao, Xiaohui Li, Gongning Shi
Background: Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) was reported to be aberrantly upregulated and promote esophageal squamous cell carcinoma (ESCC) cell progression. Nevertheless, the molecular mechanism of NEAT1 involved in the competing endogenous RNA (ceRNA) regulatory network in ESCC progression remains poorly defined. Methods: The expressions of NEAT1, miR-129, and C-terminal-binding protein 2 (CTBP2) in ESCC cells were examined by qRT-PCR...
2017: Disease Markers
https://www.readbyqxmd.com/read/29113206/nuclear-paraspeckle-assembly-transcript-1-promotes-the-metastasis-and-epithelial-mesenchymal-transition-of-hepatoblastoma-cells-by-inhibiting-mir-129-5p
#9
Ming-Cui Fu, Li-Qun Yuan, Ting Zhang, Xiang-Ming Yan, Yun Zhou, Hong-Liang Xia, Yi Wu, Li-Xiao Xu, Xu Cao, Jian Wang
The abnormal expression of nuclear paraspeckle assembly transcript 1 (NEAT1) may serve critical functions for the development and progression of various types of human tumor. However, the expression and biological function of NEAT1 in hepatoblastoma (HB) and the underlying mechanisms for the function of NEAT1 in HB remain largely uncharacterized. In the present study, the results of reverse transcription-quantitative polymerase chain reaction revealed that the expression of NEAT1 was significantly elevated in HB tissues...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29075020/the-rbm14-coaa-interacting-long-intergenic-non-coding-rna-paral1-regulates-adipogenesis-and-coactivates-the-nuclear-receptor-ppar%C3%AE
#10
François F Firmin, Frederik Oger, Céline Gheeraert, Julie Dubois-Chevalier, Anne-Sophie Vercoutter-Edouart, Fawaz Alzaid, Claire Mazuy, Hélène Dehondt, Jeremy Alexandre, Bruno Derudas, Quentin Dhalluin, Maheul Ploton, Alexandre Berthier, Eloise Woitrain, Tony Lefebvre, Nicolas Venteclef, François Pattou, Bart Staels, Jérôme Eeckhoute, Philippe Lefebvre
Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there is a need for a thorough understanding of the transcriptional regulatory network involved in adipose tissue pathophysiology. Recent advances in the functional annotation of the genome has highlighted the role of non-coding RNAs in cellular differentiation processes in coordination with transcription factors...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29069457/nol12-is-a-multifunctional-rna-binding-protein-at-the-nexus-of-rna-and-dna-metabolism
#11
Daniel D Scott, Christian Trahan, Pierre J Zindy, Lisbeth C Aguilar, Marc Y Delubac, Eric L Van Nostrand, Srivathsan Adivarahan, Karen E Wei, Gene W Yeo, Daniel Zenklusen, Marlene Oeffinger
To counteract the breakdown of genome integrity, eukaryotic cells have developed a network of surveillance pathways to prevent and resolve DNA damage. Recent data has recognized the importance of RNA binding proteins (RBPs) in DNA damage repair (DDR) pathways. Here, we describe Nol12 as a multifunctional RBP with roles in RNA metabolism and genome maintenance. Nol12 is found in different subcellular compartments-nucleoli, where it associates with ribosomal RNA and is required for efficient separation of large and small subunit precursors at site 2; the nucleoplasm, where it co-localizes with the RNA/DNA helicase Dhx9 and paraspeckles; as well as GW/P-bodies in the cytoplasm...
October 23, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29047232/knockdown-of-long-non-coding-rna-neat1-inhibits-proliferation-and-invasion-and-induces-apoptosis-of-osteosarcoma-by-inhibiting-mir-194-expression
#12
Heping Wang, Yanzhang Yu, Shuxin Fan, Leifeng Luo
PURPOSE: Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated as an oncogene in the development and progression of osteosarcoma. This study aims to explore the mechanism of NEAT1 in osteosarcoma. MATERIALS AND METHODS: Expressions of NEAT1 and miR-194 in osteosarcoma tissues and cells were detected by quantitative real-time PCR. The effects of NEAT1 knockdown or miR-194 overexpression on cell proliferation, invasion, and apoptosis were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay, transwell invasive assay, and flow cytometry analysis, respectively...
November 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/29033980/cellular-physiological-and-pathological-aspects-of-the-long-non-coding-rna-neat1
#13
Pang-Kuo Lo, Benjamin Wolfson, Qun Zhou
BACKGROUND: The majority of mammalian genomes have been found to be transcribed into non-coding RNAs. One category of non-coding RNAs is classified as long non-coding RNAs (lncRNAs) based on their transcript sizes larger than 200 nucleotides. Growing evidence has shown that lncRNAs are not junk transcripts and play regulatory roles in multiple aspects of biological processes. Dysregulation of lncRNA expression has also been linked to diseases, in particular cancer. Therefore, studies of lncRNAs have attracted significant interest in the field of medical research...
December 2016: Frontiers in Biology
https://www.readbyqxmd.com/read/28993096/altered-expression-of-malat1-lncrna-in-nonalcoholic-steatohepatitis-fibrosis-regulates-cxcl5-in-hepatic-stellate-cells
#14
Fatjon Leti, Christophe Legendre, Christopher D Still, Xin Chu, Anthony Petrick, Glenn S Gerhard, Johanna K DiStefano
In the present study, we sought to identify long noncoding RNA (lncRNA) expression profiles in nonalcoholic steatohepatitis (NASH) patients with histologic evidence of lobular inflammation and advanced fibrosis. We profiled lncRNA expression using RNA-sequencing of wedge liver biopsies from 24 nonalcoholic fatty liver disease (NAFLD) patients with normal liver histology, 53 NAFLD patients with lobular inflammation, and 65 NAFLD patients with advanced fibrosis. Transcript profiling identified 4432 and 4057 differentially expressed lncRNAs in comparisons of normal tissue with lobular inflammation and fibrosis samples, respectively...
September 19, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28981074/paraspeckles-nuclear-nests-helping-to-raise-mature-mirnas
#15
Jacek Krol
No abstract text is available yet for this article.
October 5, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28977530/nuclear-poly-a-binding-protein-1-pabpn1-and-matrin3-interact-in-muscle-cells-and-regulate-rna-processing
#16
Ayan Banerjee, Katherine E Vest, Grace K Pavlath, Anita H Corbett
The polyadenylate binding protein 1 (PABPN1) is a ubiquitously expressed RNA binding protein vital for multiple steps in RNA metabolism. Although PABPN1 plays a critical role in the regulation of RNA processing, mutation of the gene encoding this ubiquitously expressed RNA binding protein causes a specific form of muscular dystrophy termed oculopharyngeal muscular dystrophy (OPMD). Despite the tissue-specific pathology that occurs in this disease, only recently have studies of PABPN1 begun to explore the role of this protein in skeletal muscle...
October 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28946559/neat1-negatively-regulates-mir-218-expression-and-promotes-breast-cancer-progression
#17
Dahua Zhao, Ying Zhang, Nana Wang, Ning Yu
BACKGROUND: Studies have shown that lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is related to breast cancer progression, however, the role of NEAT1 remains largely unknown. The aim of the current study was to further investigate the function of NEAT1 involved in breast cancer. METHODS: QRT-PCR was used to analyze lncRNA NEAT1 expression in breast cancer tissues and determine the association between NEAT1 and clinicopathologic features in patients. Kaplan-Meier analysis and the log-rank test were used to establish the relationship between NEAT1 and overall survival...
September 7, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28864835/neat1-accelerates-the-progression-of-liver-fibrosis-via-regulation-of-microrna-122-and-kruppel-like-factor-6
#18
Fujun Yu, Zhe Jiang, Bicheng Chen, Peihong Dong, Jianjian Zheng
Long non-coding RNAs (lncRNAs) have been reported to be involved in many important biological processes including proliferation, apoptosis, differentiation, and survival. Recently, nuclear paraspeckle assembly transcript 1 (NEAT1), a novel lncRNA, serves as a crucial regulator in tumors. However, the biological role of NEAT1 in liver fibrosis is largely unknown. In this study, the role of NEAT1 was explored in primary mouse hepatic stellate cells (HSCs) and carbon tetrachloride (CCl4)-induced mouse liver fibrosis models...
November 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28846091/neat1-scaffolds-rna-binding-proteins-and-the-microprocessor-to-globally-enhance-pri-mirna-processing
#19
Li Jiang, Changwei Shao, Qi-Jia Wu, Geng Chen, Jie Zhou, Bo Yang, Hairi Li, Lan-Tao Gou, Yi Zhang, Yangming Wang, Gene W Yeo, Yu Zhou, Xiang-Dong Fu
MicroRNA (miRNA) biogenesis is known to be modulated by a variety of RNA-binding proteins (RBPs), but in most cases, individual RBPs appear to influence the processing of a small subset of target miRNAs. Here, we report that the RNA-binding NONO-PSF heterodimer binds a large number of expressed pri-miRNAs in HeLa cells to globally enhance pri-miRNA processing by the Drosha-DGCR8 Microprocessor. NONO and PSF are key components of paraspeckles organized by the long noncoding RNA (lncRNA) NEAT1. We further demonstrate that NEAT1 also has a profound effect on global pri-miRNA processing...
October 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28810932/lncrna-neat1-promotes-growth-and-metastasis-of-cholangiocarcinoma-cells
#20
Cheng Zhang, Jing-Yi Li, Fu-Zhou Tian, Gang Zhao, Hai Hu, Yue-Feng Ma, Yu-Long Yang
Long non-coding RNAs (lncRNAs) are known to play important roles in cancers. However, little is known about lncRNAs incholangiocarcinoma (CCA), a cholangiocyte malignancy with poor prognosis. We investigated the role of Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) lncRNA in promoting cholangiocarcinoma (CCA). qRT-PCR analysis of patient samples showed that NEAT1 expression was higher in CCA tumors than matched adjacent non-tumor tissue. NEAT1 levels were also higher in CCA cell lines than a normal biliary epithelium cell line (HIBEpic)...
August 15, 2017: Oncology Research
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