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https://www.readbyqxmd.com/read/29768216/the-rna-exosome-adaptor-zfc3h1-functionally-competes-with-nuclear-export-activity-to-retain-target-transcripts
#1
Toomas Silla, Evdoxia Karadoulama, Dawid Mąkosa, Michal Lubas, Torben Heick Jensen
Mammalian genomes are promiscuously transcribed, yielding protein-coding and non-coding products. Many transcripts are short lived due to their nuclear degradation by the ribonucleolytic RNA exosome. Here, we show that abolished nuclear exosome function causes the formation of distinct nuclear foci, containing polyadenylated (pA+ ) RNA secluded from nucleocytoplasmic export. We asked whether exosome co-factors could serve such nuclear retention. Co-localization studies revealed the enrichment of pA+ RNA foci with "pA-tail exosome targeting (PAXT) connection" components MTR4, ZFC3H1, and PABPN1 but no overlap with known nuclear structures such as Cajal bodies, speckles, paraspeckles, or nucleoli...
May 15, 2018: Cell Reports
https://www.readbyqxmd.com/read/29706968/long-non-coding-rnas-novel-players-in-regulation-of-immune-response-upon-herpesvirus-infection
#2
REVIEW
Waqas Ahmed, Zheng-Fei Liu
Herpesviruses have developed a variety of sophisticated immune evasion strategies to establish lifelong latent infection, including the use of long non-coding RNAs (lncRNAs). In this review, we summarize the lncRNA action modes, i.e., RNA-protein, RNA-RNA, and RNA-DNA interactions, involved in regulating important aspects of immunity by controlling gene expression at various stages. Upon herpesvirus infection, host lncRNAs, such as nuclear paraspeckle assembly transcript 1, negative regulator of antiviral, and B-cell integration cluster have been functionally characterized as negative or positive antiviral regulators in the immune response...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29662142/the-hepatitis-delta-virus-accumulation-requires-paraspeckle-components-and-affects-neat1-level-and-psp1-localization
#3
Yasnee Beeharry, Gabrielle Goodrum, Christian J Imperiale, Martin Pelchat
The Hepatitis Delta Virus (HDV) relies mainly on host proteins for its replication. We previously identified that PSF and p54nrb associate with the HDV RNA genome during viral replication. Together with PSP1, these proteins are part of paraspeckles, which are subnuclear bodies nucleated by the long non-coding RNA NEAT1. In this work, we established the requirement for PSF, p54nrb and PSP1 in HDV replication using RNAi-mediated knockdown in HEK-293 cells replicating the HDV RNA genome. We determined that HDV replication induces the delocalization of PSP1 to cytoplasmic foci containing PABP and increases NEAT1 level causing an enlargement of NEAT1 foci...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29593326/pspc1-mediates-tgf-%C3%AE-1-autocrine-signalling-and-smad2-3-target-switching-to-promote-emt-stemness-and-metastasis
#4
Hsi-Wen Yeh, En-Chi Hsu, Szu-Shuo Lee, Yaw-Dong Lang, Yuh-Charn Lin, Chieh-Yu Chang, Suz-Yi Lee, De-Leung Gu, Jou-Ho Shih, Chun-Ming Ho, Chian-Feng Chen, Chiung-Tong Chen, Pang-Hsien Tu, Ching-Feng Cheng, Ruey-Hwa Chen, Ruey-Bing Yang, Yuh-Shan Jou
Activation of metastatic reprogramming is critical for tumour metastasis. However, more detailed knowledge of the underlying mechanism is needed to enable targeted intervention. Here, we show that paraspeckle component 1 (PSPC1), identified in an aberrant 13q12.11 locus, is upregulated and associated with poor survival in patients with cancer. PSPC1 promotes tumorigenesis, epithelial-to-mesenchymal transition (EMT), stemness and metastasis in multiple cell types and in spontaneous mouse cancer models. PSPC1 is the master activator for transcription factors of EMT and stemness and accompanies c-Myc activation to facilitate tumour growth...
April 2018: Nature Cell Biology
https://www.readbyqxmd.com/read/29593320/paraspeckle-factor-turns-tgf-%C3%AE-1-pro-metastatic
#5
Fernando Salvador, Roger R Gomis
No abstract text is available yet for this article.
April 2018: Nature Cell Biology
https://www.readbyqxmd.com/read/29565706/lncrna-neat1-let-7a-5p-axis-regulates-the-cisplatin-resistance-in-nasopharyngeal-carcinoma-by-targeting-rsf-1-and-modulating-the-ras-mapk-pathway
#6
Fei Liu, Yong Tai, Jiqing Ma
The long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) was reported to be upregulated and be involved in oncogenic growth and drug resistance in nasopharyngeal carcinoma (NPC). However, the exact roles of NEAT1 and its underlying mechanisms in the drug resistance of NPC remain largely unclear. In this study, the expressions of NEAT1, let-72-5p and Rsf-1 mRNA were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The effects of NEAT1 and let-72-5p on cell proliferation and cisplatin resistance of NPC cells were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and 5-ethynyl-20-deoxyuridine (EdU) assay...
March 22, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29544562/long-non-coding-rna-neat1-regulates-the-proliferation-migration-and-invasion-of-gastric-cancer-cells-via-targeting-mir-335-5p-rock1-axis
#7
Hao Wang, Mingxia Zhang, Guoping Sun
This study aimed to elucidate the roles of long non-coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) in gastric cancer. The expression of NEAT1 in gastric cancer tissues and cells was determined. NEAT1 was then overexpressed and suppressed in BGC-823 cells in vitro to further explore the effects of NEAT1 on cell proliferation, migration, and invasion. In addition, the regulatory relationship among NEAT1, miR-335-5p and Rho Associated Coiled-Coil Containing Protein Kinase 1 (ROCK1) was investigated...
March 5, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29530979/crystal-structure-of-a-sfpq-pspc1-heterodimer-provides-insights-into-preferential-heterodimerization-of-human-dbhs-family-proteins
#8
Jie Huang, G Patricia Casas Garcia, Matthew A Perugini, Archa H Fox, Charles S Bond, Mihwa Lee
Members of the Drosophila behavior human splicing (DBHS) protein family are nuclear proteins implicated in many layers of nuclear functions, including RNA biogenesis as well as DNA repair. Definitive of the DBHS protein family, the conserved DBHS domain provides a dimerization platform that is critical for the structural integrity and function of these proteins. The three human DBHS proteins, splicing factor proline- and glutamine-rich (SFPQ), paraspeckle component 1 (PSPC1), and non-POU domain-containing octamer-binding protein (NONO), form either homo- or heterodimers; however, the relative affinity and mechanistic details of preferential heterodimerization are yet to be deciphered...
April 27, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29515109/neat1_2-functions-as-a-competing-endogenous-rna-to-regulate-atad2-expression-by-sponging-microrna-106b-5p-in-papillary-thyroid-cancer
#9
Wei Sun, Xiabin Lan, Hao Zhang, Zhihong Wang, Wenwu Dong, Liang He, Ting Zhang, Ping Zhang, Jinhao Liu, Yuan Qin
Nuclear paraspeckle assembly transcript 1 (NEAT1), a long non-coding RNA (lncRNA), is a core structural component of paraspeckles and is essential for paraspeckle formation. NEAT1 comprises two different isoforms: NEAT1_1 (3.7 kb) and NEAT1_2 (23 kb). Recently, NEAT1 has been shown to have oncogenic roles and to facilitate tumorigenesis in various human cancers. However, the function of NEAT1 in papillary thyroid cancer (PTC) is not well understood. The relative expression levels of NEAT1_2, ATPase family AAA domain-containing protein 2 (ATAD2), and microRNA-106b-5p (miR-106b-5p) were assessed via quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR)...
March 7, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29483655/rna-dependent-chromatin-targeting-of-tet2-for-endogenous-retrovirus-control-in-pluripotent-stem-cells
#10
Diana Guallar, Xianju Bi, Jose Angel Pardavila, Xin Huang, Carmen Saenz, Xianle Shi, Hongwei Zhou, Francesco Faiola, Junjun Ding, Phensinee Haruehanroengra, Fan Yang, Dan Li, Carlos Sanchez-Priego, Arven Saunders, Feng Pan, Victor Julian Valdes, Kevin Kelley, Miguel G Blanco, Lingyi Chen, Huayan Wang, Jia Sheng, Mingjiang Xu, Miguel Fidalgo, Xiaohua Shen, Jianlong Wang
Ten-eleven translocation (TET) proteins play key roles in the regulation of DNA-methylation status by oxidizing 5-methylcytosine (5mC) to generate 5-hydroxymethylcytosine (5hmC), which can both serve as a stable epigenetic mark and participate in active demethylation. Unlike the other members of the TET family, TET2 does not contain a DNA-binding domain, and it remains unclear how it is recruited to chromatin. Here we show that TET2 is recruited by the RNA-binding protein Paraspeckle component 1 (PSPC1) through transcriptionally active loci, including endogenous retroviruses (ERVs) whose long terminal repeats (LTRs) have been co-opted by mammalian genomes as stage- and tissue-specific transcriptional regulatory modules...
March 2018: Nature Genetics
https://www.readbyqxmd.com/read/29424086/circadian-processes-in-the-rna-life-cycle
#11
REVIEW
Manon Torres, Denis Becquet, Jean-Louis Franc, Anne-Marie François-Bellan
The circadian clock drives daily rhythms of multiple physiological processes, allowing organisms to anticipate and adjust to periodic changes in environmental conditions. These physiological rhythms are associated with robust oscillations in the expression of at least 30% of expressed genes. While the ability for the endogenous timekeeping system to generate a 24-hr cycle is a cell-autonomous mechanism based on negative autoregulatory feedback loops of transcription and translation involving core-clock genes and their protein products, it is now increasingly evident that additional mechanisms also govern the circadian oscillations of clock-controlled genes...
May 2018: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/29394378/structural-analyses-of-neat1-lncrnas-suggest-long-range-rna-interactions-that-may-contribute-to-paraspeckle-architecture
#12
Yizhu Lin, Brigitte F Schmidt, Marcel P Bruchez, C Joel McManus
Paraspeckles are nuclear bodies that regulate multiple aspects of gene expression. The long non-coding RNA (lncRNA) NEAT1 is essential for paraspeckle formation. NEAT1 has a highly ordered spatial organization within the paraspeckle, such that its 5' and 3' ends localize on the periphery of paraspeckle, while central sequences of NEAT1 are found within the paraspeckle core. As such, the structure of NEAT1 RNA may be important as a scaffold for the paraspeckle. In this study, we used SHAPE probing and computational analyses to investigate the secondary structure of human and mouse NEAT1...
January 31, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29383970/long-non-coding-rna-nuclear-paraspeckle-assembly-transcript-1-inhibits-the-apoptosis-of-retina-m%C3%A3-ller-cells-after-diabetic-retinopathy-through-regulating-mir-497-brain-derived-neurotrophic-factor-axis
#13
Xiu-Juan Li
BACKGROUND: The role of long non-coding RNA in diabetic retinopathy, a serious complication of diabetes mellitus, has attracted increasing attention in recent years. The purpose of this study was to explore whether long non-coding RNA nuclear paraspeckle assembly transcript 1 was involved in the context of diabetic retinopathy and its underlying mechanisms. RESULTS: Our results revealed that nuclear paraspeckle assembly transcript 1 was significantly downregulated in the retina of diabetes mellitus rats...
January 1, 2018: Diabetes & Vascular Disease Research
https://www.readbyqxmd.com/read/29383422/proteomic-alterations-of-brain-subcellular-organelles-caused-by-low-dose-copper-exposure-implication-for-alzheimer-s-disease
#14
Haitao Yu, Dian Wang, Liangyu Zou, Zaijun Zhang, Hua Xu, Feiqi Zhu, Xiaohu Ren, Benhong Xu, Jianhui Yuan, Jianjun Liu, Peter S Spencer, Xifei Yang
Excessive copper intake can lead to neurotoxicity, but there is a lack of comprehensive understanding on the potential impact of copper exposure especially at a low-dose on brain. We used 3xTg-AD mice to explore the potential neurotoxicity of chronic, low-dose copper treatment (0.13 ppm copper chloride in drinking water) on behavior and the brain hippocampal mitochondrial and nuclear proteome. Low-dose copper increased the spatial memory impairment of these animals, increased accumulation of intracellular amyloid 1-42 (Aβ1-42 ), decreased ATP content, increased the positive staining of 8-hydroxyguanosine (8-OHdG), a marker of DNA oxidative damage, and caused apoptosis and a decrease in synaptic proteins...
April 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/29312630/-neat1-in-hematopoietic-stem-cells
#15
Noam Fallik, Yael Bar-Lavan, Yariv Greenshpan, Oron Goldstein, Markus Grosch, Micha Drukker, Roi Gazit
Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs (lncRNA) in HSCs. Neat1 is a lncRNA that plays a major role in the formation of sub-nuclear structures called paraspeckles, and was reported to regulate proliferation and differentiation in other cells types. We detected Neat1 expression using RNA-seq data and RT-qPCR in HSCs, progenitors and effector immune cells, by specific detection of its isoforms...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29289458/paraspeckles-where-long-noncoding-rna-meets-phase-separation
#16
REVIEW
Archa H Fox, Shinichi Nakagawa, Tetsuro Hirose, Charles S Bond
Long noncoding RNA (lncRNA) molecules are some of the newest and least understood players in gene regulation. Hence, we need good model systems with well-defined RNA and protein components. One such system is paraspeckles - protein-rich nuclear organelles built around a specific lncRNA scaffold. New discoveries show how paraspeckles are formed through multiple RNA-protein and protein-protein interactions, some of which involve extensive polymerization, and others with multivalent interactions driving phase separation...
February 2018: Trends in Biochemical Sciences
https://www.readbyqxmd.com/read/29287722/lncrna-neat1-promotes-autophagy-in-mptp-induced-parkinson-s-disease-through-stabilizing-pink1-protein
#17
Wang Yan, Zhao-Ying Chen, Jia-Qi Chen, Hui-Min Chen
BACKGROUND: Long non-coding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1) was found to be closely related to the pathological changes in brain and nervous system. However, the role of NEAT1 and its potential mechanism in Parkinson's disease (PD) largely remain uncharacterized. METHODS: In this study, PD mouse model was established by intraperitoneal injection of MPTP. The numbers of TH + neurons, NEAT1 expression and the level of PINK1, LC3-II, LC3-I protein were assessed in PD mice...
February 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29222807/long-non-coding-rna-expression-in-bladder-cancer
#18
REVIEW
Mohammad Taheri, Mir Davood Omrani, Soudeh Ghafouri-Fard
The advent of novel high-throughput sequencing methods has facilitated identification of non-coding RNAs with fundamental roles in cellular biological and pathological conditions. A group of these consisting of at least 200 nucleotides are called long non-coding RNAs (lncRNAs). Their participation in the pathogenesis of cancer has been highlighted in recent years. Bladder cancer, one of the most prevalent cancers worldwide, exhibits altered expression levels of several lncRNAs. Several in vitro and in vivo studies have assessed the effects of silencing RNAs on cancer cell phenotypes and in vivo tumor growth...
December 8, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/29207151/long-non%C3%A2-coding-nuclear-paraspeckle-assembly-transcript-1-acts-as-prognosis-biomarker-and-increases-cell-growth-and-invasion-in-cervical-cancer-by-sequestering-microrna%C3%A2-101
#19
Li Wang, Hongying Zhu
Emerging studies have focused on the essential role of long non‑coding RNAs (lncRNAs) in cervical carcinogenesis. A recent study has indicated that nuclear paraspeckle assembly transcript 1 (NEAT1) is highly expressed in the human cervical tissue. However, whether NEAT1 is involved in cervical cancer remains to be elucidated. Reverse transcription‑quantitative polymerase chain reaction results demonstrated that the expression of NEAT1 was higher in cervical cancer cells/tissues compared with that in normal human keratinocytes/tissues...
February 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29205703/lncrna-neat1-promotes-dexamethasone-resistance-in-multiple-myeloma-by-targeting-mir-193a-mcl1-pathway
#20
Yilan Wu, Han Wang
Although dexamethasone (DEX) remains a first-line agent for multiple myeloma (MM) therapy, the development of DEX resistance has become an indicator of poor prognosis in MM patients. It is thus urgent to develop strategies to restore the vulnerability of MM to DEX. This study demonstrated long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) was highly expressed in DEX-resistant myeloma cell lines, and upregulation of NEAT1 was tightly linked to poor prognosis. The in-depth study revealed that during the development of DEX resistance in these cells, the miR-193a levels were decreased, which resulted in the increased expression of the target gene myeloid cell leukemia-1 (MCL1)...
January 2018: Journal of Biochemical and Molecular Toxicology
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