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Paraspeckle

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https://www.readbyqxmd.com/read/27878295/silencing-of-the-long-non-coding-rna-neat1-suppresses-glioma-stem-like-properties-through-modulation-of-the-mir-107-cdk6-pathway
#1
Xiaoli Yang, Zheng Xiao, Xiaowei Du, Lina Huang, Ganqin Du
Developing novel strategies against glioma remains a significant challenge. Long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) significantly contributes to the progression of many human cancers, while glioma stem cells (GSCs) are believed to be responsible for tumor progression. However, whether NEAT1 alters the stem-like properties of GSC cells remains unknown. Using microbeads, CD133+ cells were isolated and were found to possess glioma stem cell properties. Using siRNA, NEAT1 was knocked down in the sorted CD133+ U87 glioma cells...
November 22, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27783096/neat1-modulates-herpes-simplex-virus-1-replication-by-regulating-viral-gene-transcription
#2
Ziqiang Wang, Ping Fan, Yiwan Zhao, Shikuan Zhang, Jinhua Lu, Weidong Xie, Yuyang Jiang, Fan Lei, Naihan Xu, Yaou Zhang
Nuclear paraspeckle assembly transcript 1 (NEAT1) is the crucial structural platform of paraspeckles, which is one type of nuclear bodies. As a stress-induced lncRNA, the expression of NEAT1 increases in response to viral infection, but little is known about the role of NEAT1 or paraspeckles in the replication of herpes simplex virus-1 (HSV-1). Here, we demonstrate that HSV-1 infection increases NEAT1 expression and paraspeckle formation in a STAT3-dependent manner. NEAT1 and other paraspeckle protein components, P54nrb and PSPC1, can associate with HSV-1 genomic DNA...
October 25, 2016: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/27774948/-alterations-and-clinical-signifecance-of-exosome-containing-innate-immunity-related-lncrnas-in-patients-of-hemorrhagic-fever-with-renal-syndrome
#3
Xuyang Zheng, Chuantao Ye, Jieru Zhao, Peiyu Bian, Ying Zhang, Zhansheng Jia
Objective To observe the alterations of innate immunity related long non-coding RNAs (lncRNAs) in exosomes extracted from the plasma of hemorrhagic fever with renal syndrome (HFRS) patients, and analyze their relationship with the disease stage and severity. Methods Exosomes were extracted from the plasma samples of HFRS patients, healthy controls and recovered HFRS patients. Transmission electronic microscopy and Western blotting were performed to confirm the efficiency of the extraction. lncRNA profiles in the different groups were determined by high-throughput sequencing...
November 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27700225/neat1-containing-paraspeckles-central-hubs-in-stress-response-and-tumor-formation
#4
Carmen Adriaens, Jean-Christophe Marine
No abstract text is available yet for this article.
October 4, 2016: Cell Cycle
https://www.readbyqxmd.com/read/27665741/paraspeckles-modulate-the-intranuclear-distribution-of-paraspeckle-associated-ctn-rna
#5
Aparna Anantharaman, Mahdieh Jadaliha, Vidisha Tripathi, Shinichi Nakagawa, Tetsuro Hirose, Michael F Jantsch, Supriya G Prasanth, Kannanganattu V Prasanth
Paraspeckles are sub-nuclear domains that are nucleated by long noncoding RNA Neat1. While interaction of protein components of paraspeckles and Neat1 is understood, there is limited information on the interaction of non-structural RNA components with paraspeckles. Here, by varying paraspeckle number and size, we investigate how paraspeckles influence the nuclear organization of their non-structural RNA component Ctn RNA. Our results show that Ctn RNA remains nuclear-retained in the absence of intact paraspeckles, suggesting that they do not regulate nuclear retention of Ctn RNA...
September 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27646274/structural-super-resolution-microscopy-analysis-of-paraspeckle-nuclear-body-organization
#6
Jason A West, Mari Mito, Satoshi Kurosaka, Toru Takumi, Chiharu Tanegashima, Takeshi Chujo, Kaori Yanaka, Robert E Kingston, Tetsuro Hirose, Charles Bond, Archa Fox, Shinichi Nakagawa
Paraspeckles are nuclear bodies built on the long noncoding RNA Neat1, which regulates a variety of physiological processes including cancer progression and corpus luteum formation. To obtain further insight into the molecular basis of the function of paraspeckles, we performed fine structural analyses of these nuclear bodies using structural illumination microscopy. Notably, paraspeckle proteins are found within different layers along the radially arranged bundles of Neat1 transcripts, forming a characteristic core-shell spheroidal structure...
September 26, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27646270/shedding-light-on-paraspeckle-structure-by-super-resolution-microscopy
#7
Shi-Bin Hu, Run-Wen Yao, Ling-Ling Chen
The nuclear body paraspeckle is built on the lncRNA Neat1 and plays important roles in gene regulation. In this issue, West et al. (2016. J. Cell Biol http://dx.doi.org/10.1083/jcb.201601071) use super-resolution structured illumination microscopy to show that paraspeckles are organized in a core-shell spheroidal structure composed of Neat1 and seven proteins.
September 26, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27556696/knockdown-of-neat1-restrained-the-malignant-progression-of-glioma-stem-cells-by-activating-microrna-let-7e
#8
Wei Gong, Jian Zheng, Xiaobai Liu, Jun Ma, Yunhui Liu, Yixue Xue
Nuclear paraspeckle assembly transcript 1 (NEAT1), a long non-coding RNA, promotes oncogenesis in various tumors, including human gliomas. Herein, we studied the expression and function of NEAT1 in glioma stem cells (GSCs). Quantitative real-time PCR demonstrated that NEAT1 was upregulated in GSCs. NEAT1 knockdown inhibited GSC cell proliferation, migration and invasion and promoted GSC apoptosis. A potential binding region between NEAT1 and microRNA let-7e was confirmed by dual-luciferase assays. Upregulation of NEAT1 reduced the expression of let-7e, and there was reciprocal repression between NEAT1 and let-7e in an Argonaute 2-dependent manner...
August 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/27526954/localized-movement-and-levels-of-53bp1-protein-are-changed-by-%C3%AE-irradiation-in-pml-deficient-cells
#9
Soňa Legartová, Petra Sehnalová, Barbora Malyšková, Thomas Küntziger, Philippe Collas, Dušan Cmarko, Ivan Raška, Dmitry V Sorokin, Stanislav Kozubek, Eva Bártová
We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and γ-radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after γ-irradiation...
November 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27446393/overexpression-of-lncrna-neat1-mitigates-multidrug-resistance-by-inhibiting-abcg2-in-leukemia
#10
Caihua Gao, Jianying Zhang, Qingyan Wang, Chunhua Ren
Leukemia is a heterogeneous clonal disorder in which early hematopoietic cells fail to differentiate and do not undergo programmed cell death or apoptosis. Less than one-third of adult patients with leukemia are managed using current therapies due to the emergence of multidrug resistance (MDR), emphasizing the need for newer and more robust approaches. Recent reports have suggested that long non-coding RNAs (lncRNAs) contribute to selective gene expression and, hence, could be manipulated effectively to halt the progression of cancer...
August 2016: Oncology Letters
https://www.readbyqxmd.com/read/27441387/circadian-rna-expression-elicited-by-3-utr-iralu-paraspeckle-associated-elements
#11
Manon Torres, Denis Becquet, Marie-Pierre Blanchard, Séverine Guillen, Bénédicte Boyer, Mathias Moreno, Jean-Louis Franc, Anne-Marie François-Bellan
Paraspeckles are nuclear bodies form around the long non-coding RNA, Neat1, and RNA-binding proteins. While their role is not fully understood, they are believed to control gene expression at a post-transcriptional level by means of the nuclear retention of mRNA containing in their 3'-UTR inverted repeats of Alu sequences (IRAlu). In this study, we found that, in pituitary cells, all components of paraspeckles including four major proteins and Neat1 displayed a circadian expression pattern. Furthermore the insertion of IRAlu at the 3'-UTR of the EGFP cDNA led to a rhythmic circadian nuclear retention of the egfp mRNA that was lost when paraspeckles were disrupted whereas insertion of a single antisense Alu had only a weak effect...
2016: ELife
https://www.readbyqxmd.com/read/27378782/inverted-repeat-alu-elements-in-the-human-lincrna-p21-adopt-a-conserved-secondary-structure-that-regulates-rna-function
#12
Isabel Chillón, Anna M Pyle
LincRNA-p21 is a long intergenic non-coding RNA (lincRNA) involved in the p53-mediated stress response. We sequenced the human lincRNA-p21 (hLincRNA-p21) and found that it has a single exon that includes inverted repeat Alu elements (IRAlus). Sense and antisense Alu elements fold independently of one another into a secondary structure that is conserved in lincRNA-p21 among primates. Moreover, the structures formed by IRAlus are involved in the localization of hLincRNA-p21 in the nucleus, where hLincRNA-p21 colocalizes with paraspeckles...
July 4, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27376578/p53-induces-formation-of-neat1-lncrna-containing-paraspeckles-that-modulate-replication-stress-response-and-chemosensitivity
#13
Carmen Adriaens, Laura Standaert, Jasmine Barra, Mathilde Latil, Annelien Verfaillie, Peter Kalev, Bram Boeckx, Paul W G Wijnhoven, Enrico Radaelli, William Vermi, Eleonora Leucci, Gaëlle Lapouge, Benjamin Beck, Joost van den Oord, Shinichi Nakagawa, Tetsuro Hirose, Anna A Sablina, Diether Lambrechts, Stein Aerts, Cédric Blanpain, Jean-Christophe Marine
In a search for mediators of the p53 tumor suppressor pathway, which induces pleiotropic and often antagonistic cellular responses, we identified the long noncoding RNA (lncRNA) NEAT1. NEAT1 is an essential architectural component of paraspeckle nuclear bodies, whose pathophysiological relevance remains unclear. Activation of p53, pharmacologically or by oncogene-induced replication stress, stimulated the formation of paraspeckles in mouse and human cells. Silencing Neat1 expression in mice, which prevents paraspeckle formation, sensitized preneoplastic cells to DNA-damage-induced cell death and impaired skin tumorigenesis...
August 2016: Nature Medicine
https://www.readbyqxmd.com/read/27338628/the-long-non-coding-rnas-in-neurodegenerative-diseases-novel-mechanisms-of-pathogenesis
#14
Paola Riva, Antonia Ratti, Marco Venturin
BACKGROUND: Long-non-coding RNAs (lncRNAs), RNA molecules longer than 200 nucleotides, have been involved in several biological processes and in a growing number of diseases, controlling gene transcription, pre-mRNA processing, the transport of mature mRNAs to specific cellular compartments, the regulation of mRNA stability, protein translation and turnover. The fundamental role of lncRNAs in central nervous system (CNS) is becoming increasingly evident. LncRNAs are abundantly expressed in mammalian CNS in a specific spatio-temporal manner allowing a quick response to environmental/molecular changes...
June 22, 2016: Current Alzheimer Research
https://www.readbyqxmd.com/read/27315396/functional-conservation-of-the-lncrna-neat1-in-the-ancestrally-diverged-marsupial-lineage-evidence-for-neat1-expression-and-associated-paraspeckle-assembly-during-late-gestation-in-the-opossum-monodelphis-domestica
#15
Guillaume Cornelis, Sylvie Souquere, Cécile Vernochet, Thierry Heidmann, Gérard Pierron
Long non-coding RNAs (lncRNAs) are widely expressed and play various roles in cell homeostasis. However, because of their low conservation at the sequence level, recapitulating lncRNA evolutionary history is often challenging. While performing an ultrastructural analysis of viral particles present in uterine glands of gestating opossum females, we serendipitously noticed the presence of numerous structures similar to paraspeckles, nuclear bodies which in human and mouse cells are assembled around an architectural NEAT1/MENϵ/β lncRNA...
September 2016: RNA Biology
https://www.readbyqxmd.com/read/27221610/altered-expression-of-the-long-noncoding-rna-neat1-in-huntington-s-disease
#16
Jun-Sang Sunwoo, Soon-Tae Lee, Wooseok Im, Mijung Lee, Jung-Ick Byun, Keun-Hwa Jung, Kyung-Il Park, Ki-Young Jung, Sang Kun Lee, Kon Chu, Manho Kim
Huntington's disease (HD) is a devastating neurodegenerative disease caused by cytosine-adenine-guanine trinucleotide repeat expansion in the huntingtin gene. Growing evidence supports the regulatory functions of long noncoding RNAs (lncRNAs) in the disease process, but little is known about the association between lncRNAs and neuronal death in HD. Here, we evaluated the altered expression profiles of lncRNA in HD by using microarrays. Among dysregulated lncRNAs, we focused on the upregulation of nuclear paraspeckle assembly transcript 1 (NEAT1)...
May 25, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27147820/neat1-is-required-for-survival-of-breast-cancer-cells-through-fus-and-mir-548
#17
Hao Ke, Limin Zhao, Xu Feng, Haibo Xu, Li Zou, Qin Yang, Xiaosan Su, Lingtao Peng, Baowei Jiao
Increasing evidence shows that long noncoding RNAs (lncRNAs) have important roles in the regulation of multiple cellular processes, including cell division, cell growth, and apoptosis, as well as cancer metastasis and neurological disease progression; however, the mechanism of how lncRNAs regulate these processes is not well established. In this study, we demonstrated that downregulating the expression of the lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) in breast cancer cells inhibited cell growth and induced cell apoptosis...
2016: Gene Regulation and Systems Biology
https://www.readbyqxmd.com/read/27075229/hur-regulated-lncrna-neat1-stability-in-tumorigenesis-and-progression-of-ovarian-cancer
#18
Yiqing Chai, Jie Liu, Zhikun Zhang, Liwei Liu
Long noncoding RNAs (lncRNAs) have recently emerged as pivotal regulators in governing fundamental biological processes, as well as in tumorigenesis. The nuclear paraspeckle assembly transcript 1 (NEAT1) is one of the most highly regulated lncRNAs in recent genomic datasets, however, its biological role and regulatory mechanism in ovarian cancer (OC) development and progression are poorly defined. In this study, we identified that NEAT1 was up-regulated in OC patients and cell lines, and its expression was associated with the FIGO stage and lymph node metastasis...
July 2016: Cancer Medicine
https://www.readbyqxmd.com/read/27064654/structure-dynamics-and-interaction-of-p54-nrb-nono-rrm1-with-5-splice-site-rna-sequence
#19
Jean-Baptiste Duvignaud, Mikaël Bédard, Takashi Nagata, Yutaka Muto, Shigeyuki Yokoyama, Stéphane M Gagné, Michel Vincent
p54(nrb)/NonO is a nuclear RNA-binding protein involved in many cellular events such as pre-mRNA processing, transcription, and nuclear retention of hyper-edited RNAs. In particular, it participates in the splicing process by directly binding the 5' splice site of pre-mRNAs. The protein also concentrates in a nuclear body called paraspeckle by binding a G-rich segment of the ncRNA NEAT1. The N-terminal section of p54(nrb)/NonO contains tandem RNA recognition motifs (RRMs) preceded by an HQ-rich region including a threonine residue (Thr15) whose phosphorylation inhibits its RNA binding ability, except for G-rich RNAs...
May 10, 2016: Biochemistry
https://www.readbyqxmd.com/read/27034068/expression-profile-of-long-non-coding-rnas-in-serum-of-patients-with-multiple-sclerosis
#20
Massimo Santoro, Viviana Nociti, Matteo Lucchini, Chiara De Fino, Francesco Antonio Losavio, Massimiliano Mirabella
Multiple sclerosis (MS) is a chronic progressive inflammatory disease of the central nervous system (CNS) that leads to severe neurological disability. There is an interest in potential biomarkers that could provide information predicting disease activity and progression. Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of various human disorders, such as oncologic, cardiovascular, and neurodegenerative diseases. No studies have so far explored a potential link between lncRNAs and MS pathology...
May 2016: Journal of Molecular Neuroscience: MN
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