keyword
MENU ▼
Read by QxMD icon Read
search

IL-21

keyword
https://www.readbyqxmd.com/read/29229565/gm-csf-producing-autoreactive-cd4-t-cells-in-type-1-diabetes
#1
Jan Knoop, Anita Gavrisan, Denise Kuehn, Julia Reinhardt, Melanie Heinrich, Markus Hippich, Anne Eugster, Christian Ockert, Anette-Gabriele Ziegler, Ezio Bonifacio
The phenotype of autoreactive T cells in type 1 diabetes is described as Th1, Th17 and/or Th21, but is largely uncharacterized. We combined multi-parameter cytokine profiling and proliferation, and identified GM-CSF producing cells as a component of the response to beta cell autoantigens proinsulin and GAD65. Overall cytokine profiles of CD4+ T cell were not altered in type 1 diabetes. In contrast, patients with recent onset type 1 diabetes had increased frequencies of proinsulin-responsive CD4+CD45RA- T cells producing GM-CSF (p=0...
December 8, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/29220875/interleukin-21-receptor-signaling-is-not-critically-required-for-imiquimod-induced-psoriasiform-dermatitis-in-mice
#2
Hee Joo Kim, Sung Hee Kim, Tae-Gyun Kim, Je Yun Park, Minseok Lee, Dae Suk Kim, Min-Geol Lee
Psoriasis is largely mediated by interleukin (IL)-23/ T helper (Th) 17 axis, and IL-21 is a pleiotropic cytokine expressed by Th17 cells. Despite previously reported possible pathogenic roles of IL-21 in human psoriasis, we found that IL-21 receptor (IL-21R) signaling was not crucial for imiquimod-induced psoriatic inflammation, using IL-21R-/- mice. The severity of imiquimod-induced psoriatic manifestation and pro-inflammatory Th17 cytokine levels, IL-17A-producing γδ T cells and CD4+ T cells, and in vitro IL-17A production by γδ T cells after IL-23 stimulation was comparable between wild-type and IL-21R-/- mice...
December 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/29214756/serum-cytokine-profile-in-patients-with-chronic-rhinosinusitis-with-nasal-polyposis-infected-by-aspergillus-flavus
#3
Gargi Rai, Mohammad Ahmad Ansari, Sajad Ahmad Dar, Shyama Datt, Neelima Gupta, Sonal Sharma, Shafiul Haque, Vishnampettai Ganapathysubramanian Ramachandran, Arpeeta Mazumdar, Shivprakash Rudramurthy, Arunaloke Chakrabarti, Shukla Das
BACKGROUND: Fungi, especially Aspergillus flavus, can cause chronic rhinosinusitis with nasal polyposis and modulate host innate immune components. The objective of this study was to examine the serum levels of T helper (Th) cell subset Th1, Th2, and Th17 cytokines and total IgE in patients having chronic rhinosinusitis with nasal polyposis and Aspergillus flavus infection. METHODS: A case-control study including 40 patients with chronic rhinosinusitis with nasal polyposis and 20 healthy controls was conducted...
March 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29212666/stat4-and-t-bet-control-follicular-helper-t-cell-development-in-viral-infections
#4
Jason S Weinstein, Brian J Laidlaw, Yisi Lu, Jessica K Wang, Vincent P Schulz, Ningcheng Li, Edward I Herman, Susan M Kaech, Patrick G Gallagher, Joe Craft
Follicular helper T (Tfh) cells promote germinal center (GC) B cell survival and proliferation and guide their differentiation and immunoglobulin isotype switching by delivering contact-dependent and soluble factors, including IL-21, IL-4, IL-9, and IFN-γ. IL-21 and IFN-γ are coexpressed by Tfh cells during viral infections, but transcriptional regulation of these cytokines is not completely understood. In this study, we show that the T helper type 1 cell (Th1 cell) transcriptional regulators T-bet and STAT4 are coexpressed with Bcl6 in Tfh cells after acute viral infection, with a temporal decline in T-bet in the waning response...
December 6, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29196968/nk-cell-therapy-after-hematopoietic-stem-cell-transplantation-can-we-improve-anti-tumor-effect
#5
REVIEW
Catharina H M J Van Elssen, Stefan O Ciurea
After decades since the discovery of natural killer (NK) cells as potential effector cells fighting malignantly transformed and virally infected cells, little progress has been made in their clinical application. This yet unrealized therapeutic effect is presumably, at least in part, due to low numbers of functional NK cells that could be obtained from the peripheral blood relative to tumor burden. Our group hypothesized that a relatively small NK cell number to targeted malignant cells is the cause of a lack of clinical effect...
December 1, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/29194614/immunomodulatory-effects-of-foreskin-mesenchymal-stromal-cells-fsk-mscs-on-natural-killer-nk-cells
#6
Mehdi Najar, Mohammad Fayyad-Kazan, Nathalie Meuleman, Dominique Bron, Hussein Fayyad-Kazan, Laurence Lagneaux
Foreskin-mesenchymal stromal cells (FSK-MSCs) are immune-privileged thus making them valuable immunotherapeutic cell product. Characterization of the relationship between FSK-MSCs and natural killer (NK) cells is essential to improve cell-based therapy. In the present study, we studied for the first time FSK-MSCs-NK interaction and showed that the result of such cross talk was robustly dependent on the type of cytokines (IL-2, IL-12, IL-15 and IL-21) employed to activate NK cells. Distinctly activated-NK cells showed uneven cytotoxicity against FSK-MSCs, triggering their death in fine...
December 1, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29192391/il-21-as-a-predictor-of-sustained-virologic-response-in-patients-with-chronic-hepatitis-c-virus-infection
#7
Halla Mohamed Ragab, Nabila Abd El Maksoud, Mona A Amin, Mohab H Halim, Nour A Abdulla, Abdelkarim Kamel, Shorouk M Moussa
Hepatitis C virus (HCV) infection is a significant public health problem. The crucial role of interleukin (IL)-21 in HCV has been established. Thus, we aimed to investigate the association of serum IL-21 levels with the virological response to interferon (IFN)-based therapy in a group of Egyptian patients with chronic hepatitis C (CHC). Clinical data were collected from 58 HCV-positive Egyptian patients treated with IFN/ribavirin therapy and 10 non-HCV-infected healthy subjects. Liver and renal function tests, complete blood count, viral markers, and pretreatment IL-21 levels were determined in all patients and healthy controls...
December 1, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29192177/up-regulation-of-interleukin-21-contributes-to-liver-pathology-of-schistosomiasis-by-driving-gc-immune-responses-and-activating-hscs-in-mice
#8
Yanyan Wang, Cai Lin, Yun Cao, Zhongliang Duan, Zhixun Guan, Jing Xu, Xing-Quan Zhu, Chaoming Xia
The pathology of schistosome egg-induced liver granuloma, fibrosis and eventually liver scarring is complicated. CD4+ helper T (Th) cells play critical roles in both host humoral immunity and cellular immunity against parasitic infection and immunopathology in schistosomiasis. Follicular helper T (Tfh) cells are another specialized subset of Th cells and involved in infectious diseases. However, the immune regulatory mechanism of Tfh cells in severe liver pathology of schistosomiasis is still poorly understood...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29186193/rig-i-like-receptor-activation-by-dengue-virus-drives-follicular-t-helper-cell-formation-and-antibody-production
#9
Joris K Sprokholt, Tanja M Kaptein, John L van Hamme, Ronald J Overmars, Sonja I Gringhuis, Teunis B H Geijtenbeek
Follicular T helper cells (TFH) are fundamental in orchestrating effective antibody-mediated responses critical for immunity against viral infections and effective vaccines. However, it is unclear how virus infection leads to TFH induction. We here show that dengue virus (DENV) infection of human dendritic cells (DCs) drives TFH formation via crosstalk of RIG-I-like receptor (RLR) RIG-I and MDA5 with type I Interferon (IFN) signaling. DENV infection leads to RLR-dependent IKKε activation, which phosphorylates IFNα/β receptor-induced STAT1 to drive IL-27 production via the transcriptional complex ISGF3...
November 29, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29185590/short-term-effects-of-stored-homologous-red-blood-cell-transfusion-on-cardiorespiratory-function-and-inflammation-an-experimental-study-in-a-hypovolemia-model
#10
S Biagini, C S Dale, J M Real, E S Moreira, C R R Carvalho, G P P Schettino, S Wendel, L C P Azevedo
The pathophysiological mechanisms associated with the effects of red blood cell (RBC) transfusion on cardiopulmonary function and inflammation are unclear. We developed an experimental model of homologous 14-days stored RBC transfusion in hypovolemic swine to evaluate the short-term effects of transfusion on cardiopulmonary system and inflammation. Sixteen healthy male anesthetized swine (68±3.3 kg) were submitted to controlled hemorrhage (25% of blood volume). Two units of non-filtered RBC from each animal were stored under blood bank conditions for 14 days...
November 17, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29180321/-a-recombinant-adenovirus-vector-carrying-murine-interleukin-21-gene-controls-chronic-hbv-infection-in-mice
#11
Xue-Ping Gao, Yang Zhou, Xin-Chun Zheng, Xuan Yi, Li-Bo Tang, Jin-Lin Hou, Yong-Yin Li
OBJECTIVE: To investigate the effect of an adenovirus vector containing murine interleukin-21 gene (Ad-GFP-mIL-21) in virus clearance and on the production of HBV-specific antibodies in mice with persistent HBV infection. METHODS: ELISA and Western blot analysis were used to detect the expression of mIL-21 in the supernatant and cytoplasm of cultured HepG2.2.15 cells after infection by Ad-GFP-mIL-21. Mouse models of chronic HBV infection established by in vivo transduction with rAAV8-1...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29176747/the-crucial-roles-of-th17-related-cytokines-signal-pathways-in-m-tuberculosis-infection
#12
REVIEW
Hongbo Shen, Zheng W Chen
Interleukin-17 (IL-17), IL-21, IL-22 and IL-23 can be grouped as T helper 17 (Th17)-related cytokines because they are either produced by Th17/Th22 cells or involved in their development. Here, we review Th17-related cytokines/Th17-like cells, networks/signals and their roles in immune responses or immunity against Mycobacterium tuberculosis (Mtb) infection. Published studies suggest that Th17-related cytokine pathways may be manipulated by Mtb microorganisms for their survival benefits in primary tuberculosis (TB)...
November 27, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29175594/reduced-pten-involved-in-primary-immune-thrombocytopenia-via-contributing-to-b-cell-hyper-responsiveness
#13
Shixuan Wang, Yue Guan, Yunlong Wang, Huiyuan Li, Donglei Zhang, Mankai Ju, Yating Hao, Xuewen Song, Boyang Sun, Xueqing Dou, Renchi Yang
Phosphatase and tensin homolog (PTEN) is thought to mediate B cell activation by negatively regulating the phosphoinositide 3-kinase (PI3K) signaling pathway. This pathway is important for activation, growth, and proliferation. Although enhanced B cell receptor (BCR) signaling contributes to increased B cell activity in immune thrombocytopenia (ITP), the role of PTEN is unclear. In this study, we analyzed B cells of ITP patients using flow cytometry and found that all B cell subsets, excluding memory B cells, showed lower PTEN expression than cells from healthy controls (HCs)...
November 22, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29175261/th17-cytokine-profiling-of-colorectal-cancer-patients-with-or-without-enterovirus-71-antigen-expression
#14
Yazhen Li, Qiancheng Qiu, Zhiqiang Fan, Ping He, Huanzhu Chen, Xiaoyang Jiao
OBJECT: Th17 cytokines have been identified in several types of human cancers. In this pilot study, the expression of Th17 cytokines profiling in enteroviruses 71 (EV71) associated colorectal cancer (CRC) were explored. METHODS: 66 patients with CRC were enrolled in this study; immune- histochemical analyses were performed on cancerous tissues and adjacent non- cancerous tissues of the patients. Serum Th17 cytokines of CRC patients and healthy controls were measured using a Luminex 200 analyzer...
November 23, 2017: Cytokine
https://www.readbyqxmd.com/read/29163764/inhibition-of-autoimmune-th17-cell-responses-by-pain-killer-ketamine
#15
Jeong-Eun Lee, Jung-Man Lee, Young-Jun Park, Byung-Seok Kim, Young-Tae Jeon, Yeonseok Chung
Ketamine is widely used in animals and humans as a systemic anesthetic. Although several immune-modulatory functions of ketamine have been reported, the effects of ketamine on the differentiation of Th17 cell are unknown. We found that ketamine significantly diminished the frequency of IL-17-producers among CD4(+) T cells stimulated under Th17-skewing conditions. Mechanistic studies showed that ketamine had little effect on the production of Th17-inducing cytokines by dendritic cells and the proliferation of T cells in response to anti-CD3; however it significantly hampered IL-21 expression as well as STAT3 phosphorylation in T cells upon IL-6 stimulation...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29163547/natural-killer-cells-from-the-subcutaneous-adipose-tissue-underexpress-the-nkp30-and-nkp44-in-obese-persons-and-are-less-active-against-major-histocompatibility-complex-class-i-non-expressing-neoplastic-cells
#16
Alireza Shoae-Hassani, Maryam Behfar, Seyed Abdolreza Mortazavi-Tabatabaei, Jafar Ai, Rashin Mohseni, Amir Ali Hamidieh
There are many types of leukocytes reside in subcutaneous adipose tissue (SAT), and among them, natural killer cells (NKs) comprise a major part. We show that the NKs that reside in the SAT (adipose tissue-derived NK cells; ADNKs) of the abdominal region found with phenotypic differences from the NKs circulating in the peripheral blood derived NK cells (PBNKs). In this survey, flow cytometry phenotyping was used to study the differences between the natural cytotoxicity receptor expression on ADNKs and PBNKs of both obese and lean persons...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29163509/downregulated-il-21-response-and-t-follicular-helper-cell-exhaustion-correlate-with-compromised-cd8-t-cell-immunity-during-chronic-toxoplasmosis
#17
Magali M Moretto, SuJin Hwang, Imtiaz A Khan
CD8 T cells are important for maintaining the chronicity of Toxoplasma gondii infection. In a T. gondii encephalitis susceptible model, we recently demonstrated that CD4 T cells play an essential helper role in the maintenance of the effector response and CD8 T cell dysfunctionality was linked to CD4 T cell exhaustion. However, CD4 T cells are constituted of different subsets with various functions and the population(s) providing help to the CD8 T cells has not yet been determined. In the present study, T follicular helper cells (Tfh), which are known to be essential for B cell maturation and are one of the main sources of IL-21, were significantly increased during chronic toxoplasmosis...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29161463/the-il-21-mtor-axis-blocks-treg-differentiation-and-function-by-suppression-of-autophagy-in-patients-with-systemic-lupus-erythematosus
#18
Hiroshi Kato, Andras Perl
OBJECTIVES: The mechanistic target of rapamycin (mTOR) has become a therapeutic target in systemic lupus erythematosus (SLE). mTOR regulates normal T-cell lineage specification, including development of regulatory cells (Tregs). Therefore, we investigated whether mTOR is activated within Tregs and causes their depletion and dysfunction in patients with SLE. METHODS: Activities of mTOR complexes 1 (mTORC1) and 2 (mTORC2) were examined by phosphorylation of 4E-BP1, S6K, and Akt, respectively, in SLE patients relative to healthy control females age-matched for each experiment...
November 21, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29149703/quercetin-attenuates-collagen-induced-arthritis-by-restoration-of-th17-treg-balance-and-activation-of-heme-oxygenase-1-mediated-anti-inflammatory-effect
#19
Yan Yang, Xu Zhang, Min Xu, Xin Wu, Feipeng Zhao, Chengzhi Zhao
Quercetin (QU) has been shown obvious anti-arthritic property in pre-clinical studies or clinical studies. Howbeit, the underlying mechanism of it is still not revealed distinctly and should be gotten further insight into. QU at a dosage of 150 mg/kg was administered orally in collagen-induced arthritis rats and then the clinical symptoms were monitored. The protein levels of Th17/Treg-related cytokines were determined by ELISA, and the mRNA levels of cytokines and transcription factors associated with the Th17 and Treg phenotypes were evaluated by real-time PCR, the proportions of Th17 and Treg cells were assessed by flow cytometry...
November 14, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29147974/genotype-related-variations-in-proinflammatory-and-regulatory-cytokine-levels-in-treated-and-treatment-naive-hcv-infected-patients
#20
Rasoul Baharlou, Bizhan Romani, Seyed Jalal Kiani, Kaveh Sadeghi, Enayatollah Shadmand, Hadi Fazel, Farid Azizi Jalilian, Ebrahim Kord, Sajad Yaghoubi, Yousef Nikmanesh, Abbas Ahmadi Vasmehjani
Hepatitis C virus (HCV) modulates immune-related inflammatory responses to induce milder reactions leading to virus persistence. In this regard, the present study aimed to investigate the link between the HCV genotypes and the proinflammatory and regulatory cytokine levels. Ninety patients with hepatitis C infection (68 treatment-naive and 22 treated patients) and 76 healthy blood donors were studied. The serum levels of IFN-γ, IL-10, IL-17A, and IL-21 were measured by ELISA in the patients and healthy controls...
November 17, 2017: Medical Microbiology and Immunology
keyword
keyword
53327
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"