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https://www.readbyqxmd.com/read/23717400/the-ubiquitin-ligase-praja1-reduces-nrage-expression-and-inhibits-neuronal-differentiation-of-pc12-cells
#1
Jan Teuber, Bettina Mueller, Ryoji Fukabori, Daniel Lang, Anne Albrecht, Oliver Stork
Evidence suggests that regulated ubiquitination of proteins plays a critical role in the development and plasticity of the central nervous system. We have previously identified the ubiquitin ligase Praja1 as a gene product induced during fear memory consolidation. However, the neuronal function of this enzyme still needs to be clarified. Here, we investigate its involvement in the nerve growth factor (NGF)-induced differentiation of rat pheochromocytoma (PC12) cells. Praja1 co-localizes with cytoskeleton components and the neurotrophin receptor interacting MAGE homologue (NRAGE)...
2013: PloS One
https://www.readbyqxmd.com/read/23688634/downregulation-of-ezh2-methyltransferase-by-foxp3-new-insight-of-foxp3-into-chromatin-remodeling
#2
Zhu Shen, Ling Chen, Xiaojun Yang, Yun Zhao, Eric Pier, Xia Zhang, Xichuan Yang, Ya Xiong
Transcription factor FOXP3 (forkhead box P3) is found initially as a key regulator in regulatory T cells. Recently its expression has been demonstrated in some non-lymphoid normal and cancerous cells. Now FOXP3 has been proven to regulate cancer-related genes, especially suppressor genes in breast cancer. But the mechanisms by which FOXP3 regulates suppressor genes are not fully determined. In this study, we found the inverse correlation between FOXP3 and Ezh2, an enzyme for histone H3K27 trimethylation (H3K27me3) and a central epigenetic regulator in cancer...
October 2013: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/21513699/praja1-is-a-ubiquitin-ligase-for-the-polycomb-repressive-complex-2-proteins
#3
Muhammad Zoabi, Ronen Sadeh, Prim de Bie, Victor E Marquez, Aaron Ciechanover
Methylation of lysine 27 on histone H3 by the polycomb repressive complex 2 (PRC2) leads to transcriptional repression of genes which are critical to development. PRC2 core complex is composed of the histone methyltransferase EZH2, EED, and SUZ12. Knockdown of any of the PRC2 core subunits results in a concomitant loss of the other subunits which is mediated by the ubiquitin (Ub)-proteasome system (UPS). Inhibition of cellular methyltransferases by 3-deazaneplanocin A (DZNep) also leads to dissociation of the PRC2 complex and rapid degradation of its subunits...
May 13, 2011: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/21461837/protein-microarrays-for-the-identification-of-praja1-e3-ubiquitin-ligase-substrates
#4
Christian M Loch, Michael J Eddins, James E Strickler
Although they are the primary determinants of substrate specificity, few E3-substrate pairs have been positively identified, and few E3's profiled in a proteomic fashion. Praja1 is an E3 implicated in bone development and highly expressed in brain. Although it has been well studied relative to the majority of E3's, little is known concerning the repertoire of proteins it ubiquitylates. We sought to identify high confidence substrates for Praja1 from an unbiased proteomic profile of thousands of human proteins using protein microarrays...
June 2011: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/18786927/the-boston-type-craniosynostosis-mutation-msx2-p148h-results-in-enhanced-susceptibility-of-msx2-to-ubiquitin-dependent-degradation
#5
Won-Joon Yoon, Young-Dan Cho, Kwang-Hwi Cho, Kyung-Mi Woo, Jeong-Hwa Baek, Je-Yoel Cho, Gwan-Shik Kim, Hyun-Mo Ryoo
Boston-type craniosynostosis is caused by a single amino acid substitution, P148H, in the transcription factor MSX2. The increased binding affinity of MSX2 (P148H) to the response element has led many to hypothesize that the substitution is a gain-of-function mutation. However, there have been conflicting reports on the function of MSX2, and by extension, the nature of the P148H mutation remains unclear. In this study, we have examined the molecular mechanism of MSX2 function and the nature of the P148H mutation...
November 21, 2008: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/11959851/a-ring-finger-protein-praja1-regulates-dlx5-dependent-transcription-through-its-ubiquitin-ligase-activity-for-the-dlx-msx-interacting-mage-necdin-family-protein-dlxin-1
#6
Aya Sasaki, Yoshiko Masuda, Kazuhiro Iwai, Kyoji Ikeda, Ken Watanabe
Msx2 and Dlx5 are homeodomain proteins that play an important role in osteoblast differentiation and whose expression is induced by bone morphogenetic proteins. Recently we have identified a novel protein, Dlxin-1, that associates with these homeodomain proteins and regulates Dlx5-dependent transcriptional function (Masuda, Y., Sasaki, A., Shibuya, H., Ueno, N., Ikeda, K., and Watanabe, K. (2001) J. Biol. Chem. 276, 5331-5338). In an attempt to elucidate the molecular function of Dlxin-1, two closely related RING finger proteins, Praja1 and Neurodap-1, were isolated by yeast two-hybrid screening using the C-terminal necdin homology domain of Dlxin-1 as bait...
June 21, 2002: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/11533224/identification-of-genes-expressed-in-the-amygdala-during-the-formation-of-fear-memory
#7
O Stork, S Stork, H C Pape, K Obata
In this study we describe changes of gene expression that occur in the basolateral complex of the mouse amygdala (BLA) during the formation of fear memory. Through the combination of a behavioral training scheme with polymerase chain reaction-based expression analysis (subtractive hybridization and virtual Northern analysis) we were able to identify various gene products that are increased in expression after Pavlovian fear conditioning and are of potential significance for neural plasticity and information storage in the amygdala...
July 2001: Learning & Memory
https://www.readbyqxmd.com/read/10722742/mdm2-is-a-ring-finger-dependent-ubiquitin-protein-ligase-for-itself-and-p53
#8
S Fang, J P Jensen, R L Ludwig, K H Vousden, A M Weissman
Mdm2 has been shown to regulate p53 stability by targeting the p53 protein for proteasomal degradation. We now report that Mdm2 is a ubiquitin protein ligase (E3) for p53 and that its activity is dependent on its RING finger. Furthermore, we show that Mdm2 mediates its own ubiquitination in a RING finger-dependent manner, which requires no eukaryotic proteins other than ubiquitin-activating enzyme (E1) and an ubiquitin-conjugating enzyme (E2). It is apparent, therefore, that Mdm2 manifests an intrinsic capacity to mediate ubiquitination...
March 24, 2000: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/10500182/ring-fingers-mediate-ubiquitin-conjugating-enzyme-e2-dependent-ubiquitination
#9
K L Lorick, J P Jensen, S Fang, A M Ong, S Hatakeyama, A M Weissman
A RING finger-containing protein (AO7) that binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination was identified. Mutations of cation-coordinating residues within AO7's RING finger abolished ubiquitination, as did chelation of zinc. Several otherwise-unrelated RING finger proteins, including BRCA1, Siah-1, TRC8, NF-X1, kf-1, and Praja1, were assessed for their ability to facilitate E2-dependent ubiquitination. In all cases, ubiquitination was observed. The RING fingers were implicated directly in this activity through mutations of metal-coordinating residues or chelation of zinc...
September 28, 1999: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/10331950/cloning-and-characterization-of-rnf6-a-novel-ring-finger-gene-mapping-to-13q12
#10
D H Macdonald, D Lahiri, A Sampath, A Chase, J Sohal, N C Cross
Myeloproliferative disorders frequently show deletions or rearrangements of the long arm of chromosome 13. We report here the cloning of RNF6, a new gene that maps close to the chromosome 13 breakpoint in a case of myelofibrosis with a t(4;13)(q26;q12). RNF6 is predicted to encode a 685-amino-acid protein with a coiled-coil domain and a RING-H2 finger at the amino and carboxy terminis, respectively. In addition, we have identified a novel motif, Lys-X-X-Leu/Ile-X-X-Leu/Ile (KIL motif), that is located shortly upstream of a subset of RING-H2 proteins, including RNF6...
May 15, 1999: Genomics
https://www.readbyqxmd.com/read/9393880/praja1-a-novel-gene-encoding-a-ring-h2-motif-in-mouse-development
#11
L Mishra, R E Tully, S P Monga, P Yu, T Cai, W Makalowski, E Mezey, W J Pavan, B Mishra
As part of a cloning strategy to identify genes involved in early mouse liver development we have isolated Praja1, a gene with similar sequences to the Drosophila melanogaster gene goliath (gl) which is involved in the fate of mesodermal cells ultimately forming gut musculatures, fat body, and the heart. Praja1 is a 2.1 kb gene encoding a putative 396 amino acid ORF and includes a COOH-terminal RING-H2 domain. Using the Jackson Laboratory BSS panel, we have localized Praja1 on chromosome X at 36 cM, which may be a candidate gene for mouse sla (sex linked sideroblastic anemia), near the X inactivation center gene, Xist...
November 6, 1997: Oncogene
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