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https://www.readbyqxmd.com/read/29775645/mesenchymal-stromal-cells-induce-a-permissive-state-in-the-bone-marrow-that-enhances-g-csf-induced-hematopoietic-stem-cell-mobilization-in-mice
#1
Evert-Jan F M de Kruijf, Rob Zuijderduijn, Marjolein C Stip, Willem E Fibbe, Melissa van Pel
Mesenchymal stromal cells (MSC) support hematopoietic stem cells (HSC) in vivo and enhance HSC engraftment and hematopoietic recovery upon co-transplantation with HSC. These data have led to the hypothesis that MSC may impact the HSC niche, leading to changes in HSC retention and trafficking. We studied the effect of MSC administration on the HSC compartment in the bone marrow (BM) in mice. Following injection of MSC, HSC numbers in the BM were decreased coinciding with an increased cell cycle activity compared to PBS-injected controls...
May 15, 2018: Experimental Hematology
https://www.readbyqxmd.com/read/29774570/high-mobility-group-box-1-drives-fibrosis-progression-signaling-via-the-receptor-for-advanced-glycation-end-products-in-mice
#2
Xiaodong Ge, Elena Arriazu, Fernando Magdaleno, Daniel J Antoine, Rouchelle Dela Cruz, Neil Theise, Natalia Nieto
BACKGROUND & RATIONALE: High-mobility group box-1 (HMGB1) is a damage-associated molecular pattern (DAMP) increased in response to liver injury. Since HMGB1 is a ligand for the receptor for advanced glycation end-products (RAGE), we hypothesized that induction of HMGB1 could participate in the pathogenesis of liver fibrosis via RAGE cell-specific signaling mechanisms. RESULTS: liver HMGB1 protein expression correlated with fibrosis stage in patients with chronic Hepatitis C virus (HCV) infection, primary biliary cirrhosis (PBC) and alcoholic steatohepatitis (ASH)...
May 18, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29766024/integrating-hdad5-35-vectors-as-a-new-platform-for-hsc-gene-therapy-of-hemoglobinopathies
#3
Chang Li, Nikoletta Psatha, Hongjie Wang, Manvendra Singh, Himanshu Bhusan Samal, Wenli Zhang, Anja Ehrhardt, Zsuzsanna Izsvák, Thalia Papayannopoulou, André Lieber
We generated an integrating, CD46-targeted, helper-dependent adenovirus HDAd5/35++ vector system for hematopoietic stem cell (HSC) gene therapy. The ∼12-kb transgene cassette included a β-globin locus control region (LCR)/promoter driven human γ-globin gene and an elongation factor alpha-1 (EF1α)-mgmtP140K expression cassette, which allows for drug-controlled increase of γ-globin-expressing erythrocytes. We transduced bone marrow lineage-depleted cells from human CD46-transgenic mice and transplanted them into lethally irradiated recipients...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29765026/hematopoietic-stem-cells-can-differentiate-into-restricted-myeloid-progenitors-before-cell-division-in-mice
#4
Tatyana Grinenko, Anne Eugster, Lars Thielecke, Beáta Ramasz, Anja Krüger, Sevina Dietz, Ingmar Glauche, Alexander Gerbaulet, Malte von Bonin, Onur Basak, Hans Clevers, Triantafyllos Chavakis, Ben Wielockx
Hematopoietic stem cells (HSCs) continuously replenish all blood cell types through a series of differentiation steps and repeated cell divisions that involve the generation of lineage-committed progenitors. However, whether cell division in HSCs precedes differentiation is unclear. To this end, we used an HSC cell-tracing approach and Ki67RFP knock-in mice, in a non-conditioned transplantation model, to assess divisional history, cell cycle progression, and differentiation of adult HSCs. Our results reveal that HSCs are able to differentiate into restricted progenitors, especially common myeloid, megakaryocyte-erythroid and pre-megakaryocyte progenitors, without undergoing cell division and even before entering the S phase of the cell cycle...
May 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29764340/in-vitro-and-in-vivo-anti-cancer-activity-of-silymarin-on-oral-cancer
#5
Dong-Hoon Won, Lee-Han Kim, Boonsil Jang, In-Hyoung Yang, Hye-Jeong Kwon, Bohwan Jin, Seung Hyun Oh, Ju-Hee Kang, Seong-Doo Hong, Ji-Ae Shin, Sung-Dae Cho
Silymarin, a standardized extract from milk thistle fruits has been found to exhibit anti-cancer effects against various cancers. Here, we explored the anti-cancer activity of silymarin and its molecular target in human oral cancer in vitro and in vivo. Silymarin dose-dependently inhibited the proliferation of HSC-4 oral cancer cells and promoted caspase-dependent apoptosis. A human apoptosis protein array kit showed that death receptor 5 may be involved in silymarin-induced apoptosis, which was also shown through western blotting, immunocytochemistry, and reverse transcription-polymerase chain reaction...
May 2018: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29761840/ginsenoside-rg1-ameliorates-liver-fibrosis-via-suppressing-epithelial-to-mesenchymal-transition-and-reactive-oxygen-species-production-in-vitro-and-in-vivo
#6
Xiaoyu Wei, Yatang Chen, Wenxiang Huang
Liver fibrosis remains a major cause of morbidity and mortality with a complicated etiology and notorious complications, but there is a lack of efficacious therapeutics. Epithelial to mesenchymal transition (EMT) has a central role in the course of liver fibrosis, and thus, prevention of the development of EMT may control and even reverse liver fibrosis. This study aimed to examine the beneficial effect of ginsenoside Rg1, a phrenological active component isolated from Ginseng, and interrogate the mechanism in vitro and in vivo, with a focus on transforming growth factor-β (TGF-β)/Smad and Nrf2-mediated signaling pathways...
May 15, 2018: BioFactors
https://www.readbyqxmd.com/read/29761546/caging-nb-2-o-5-nanowires-in-pecvd-derived-graphene-capsules-toward-bendable-sodium-ion-hybrid-supercapacitors
#7
Xiangguo Wang, Qiucheng Li, Li Zhang, Zhongli Hu, Lianghao Yu, Tao Jiang, Chen Lu, Chenglin Yan, Jingyu Sun, Zhongfan Liu
Sodium-ion hybrid supercapacitors (Na-HSCs) by virtue of synergizing the merits of batteries and supercapacitors have attracted considerable attention for high-energy and high-power energy-storage applications. Orthorhombic Nb2 O5 (T-Nb2 O5 ) has recently been recognized as a promising anode material for Na-HSCs due to its typical pseudocapacitive feature, but it suffers from intrinsically low electrical conductivity. Reasonably high electrochemical performance of T-Nb2 O5 -based electrodes could merely be gained to date when sufficient carbon content was introduced...
May 14, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29760931/blood-factory-which-stem-cells
#8
Maria Teresa Esposito
Blood transfusions are often essential for treatment of severe anaemia and pregnancy complications. The unavailability of blood is a medical concern, especially in developing countries. New sources of red blood cells (RBC) are under investigation. Several studies have attempted to produce functional RBC from CD34+ haematopoietic stem cells (HSC) isolated from peripheral blood and umbilical cord blood, from embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). A recent article published in Nature Communications describes a novel model for generating RBC from a stable erythroid cell line obtained from bone marrow CD34+ haematopoietic stem cells (HSC)...
2018: BMC Hematology
https://www.readbyqxmd.com/read/29760499/hepatic-stellate-cells-secrete-ccl5-to-induce-hepatocyte-steatosis
#9
Byeong-Moo Kim, Ahmed Maher Abdelfattah, Robin Vasan, Bryan C Fuchs, Michael Y Choi
Non-alcoholic fatty liver disease (NAFLD) encompasses a wide spectrum of disease severity, starting from pure steatosis, leading to fatty inflammation labeled as non-alcoholic steatohepatitis (NASH), and finally fibrosis leading to cirrhosis. Activated hepatic stellate cells (HSCs) are known to contribute to fibrosis, but less is known about their function during NAFLD's early stages prior to fibrosis. We developed an ex vivo assay that cocultures primary HSCs from mouse models of liver disease with healthy hepatocytes to study their interaction...
May 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29755956/preleukemia-and-leukemia-initiating-cell-activity-in-inv-16-acute-myeloid-leukemia
#10
REVIEW
John Anto Pulikkan, Lucio Hernán Castilla
Acute myeloid leukemia (AML) is a collection of hematologic malignancies with specific driver mutations that direct the pathology of the disease. The understanding of the origin and function of these mutations at early stages of transformation is critical to understand the etiology of the disease and for the design of effective therapies. The chromosome inversion inv(16) is thought to arise as a founding mutation in a hematopoietic stem cell (HSC) to produce preleukemic HSCs (preL-HSCs) with myeloid bias and differentiation block, and predisposed to AML...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29754961/hematopoietic-stem-cells-but-not-multipotent-progenitors-drive-erythropoiesis-during-chronic-erythroid-stress-in-epo-transgenic-mice
#11
Rashim Pal Singh, Tatyana Grinenko, Beáta Ramasz, Kristin Franke, Mathias Lesche, Andreas Dahl, Max Gassmann, Triantafyllos Chavakis, Ian Henry, Ben Wielockx
The hematopoietic stem cell (HSC) compartment consists of a small pool of cells capable of replenishing all blood cells. Although it is established that the hematopoietic system is assembled as a hierarchical organization under steady-state conditions, emerging evidence suggests that distinct differentiation pathways may exist in response to acute stress. However, it remains unclear how different hematopoietic stem and progenitor cell subpopulations behave under sustained chronic stress. Here, by using adult transgenic mice overexpressing erythropoietin (EPO; Tg6) and a combination of in vivo, in vitro, and deep-sequencing approaches, we found that HSCs respond differentially to chronic erythroid stress compared with their closely related multipotent progenitors (MPPs)...
May 4, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29753567/junctional-adhesion-molecules-jam-b-and-jam-c-promote-autoimmune-mediated-liver-fibrosis-in-mice
#12
Edith Hintermann, Monika Bayer, Clara Benedetta Conti, Sina Fuchs, Michel Fausther, Patrick S Leung, Michel Aurrand-Lions, Richard Taubert, Josef M Pfeilschifter, Mireen Friedrich-Rust, Detlef Schuppan, Jonathan A Dranoff, M Eric Gershwin, Michael P Manns, Beat A Imhof, Urs Christen
Fibrosis remains a serious health concern in patients with chronic liver disease. We recently reported that chemically induced chronic murine liver injury triggers increased expression of junctional adhesion molecules (JAMs) JAM-B and JAM-C by endothelial cells and de novo synthesis of JAM-C by hepatic stellate cells (HSCs). Here, we demonstrate that biopsies of patients suffering from primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) or autoimmune hepatitis (AIH) display elevated levels of JAM-C on portal fibroblasts (PFs), HSCs, endothelial cells and cholangiocytes, whereas smooth muscle cells expressed JAM-C constitutively...
May 9, 2018: Journal of Autoimmunity
https://www.readbyqxmd.com/read/29753127/nanofiber-technology-in-the-ex-vivo-expansion-of-cord-blood-derived-hematopoietic-stem-cells
#13
REVIEW
Mónica Sofia Ventura Ferreira, Seyed Hadi Mousavi
Umbilical cord blood (CB) can be used as an alternative source of hematopoietic stem cells (HSCs) for transplantation in hematological and non- hematological disorders. Despite several recognized advantages the limited cell number in CB one unit still restricts its clinical use. The success of transplantation is greatly depending on the levels of total nucleated cell and CD34+ cell counts. Thus, many ex vivo strategies have been developed within the last decade in order to solve this obstacle, with more or less success, mainly determined by the degree of difficulty related with maintaining HSCs self-renewal and stemness properties after long-term expansion...
May 9, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29751513/-lactobacillus-casei-strain-shirota-enhances-the-in-vitro-antiproliferative-effect-of-geniposide-in-human-oral-squamous-carcinoma-hsc-3-cells
#14
Yu Qian, Jia-Le Song, Peng Sun, Ruokun Yi, Honglin Liu, Xia Feng, Kun-Young Park, Xin Zhao
This study investigated the enhanced antiproliferative effect of Lactobacillus casei strain Shirota (LcS) on geniposide actions in human oral squamous carcinoma HSC-3 cells. An MTT assay, flow cytometry, qPCR assay, western blot and HPLC were used for this study. The concentration of 1.0 × 10⁶ CFU/mL of LcS had no effect on the HOK normal oral epithelial cells and HSC-3 cancer cells. The 25 and 50 µg/mL geniposide concentrations also had no impact on HOK normal oral epithelial cells, but they had remarkable inhibitory effects on the growth of HSC-3 cancer cells, which are enhanced in the presence of LcS...
May 3, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29749545/genome%C3%A2-wide-identification-of-long-noncoding-rnas-in-ccl4%C3%A2-induced-liver-fibrosis-via-rna-sequencing
#15
Zhenghua Gong, Jialin Tang, Tianxin Xiang, Jiayu Lin, Chaowen Deng, Yanzhong Peng, Jie Zheng, Guoxin Hu
Liver fibrosis occurs as a result of chronic liver lesions, which may subsequently develop into liver cirrhosis and hepatocellular carcinoma. The involvement of long noncoding RNAs (lncRNAs) in liver fibrosis is being increasingly recognized. However, the exact mechanisms and functions of the majority of lncRNAs are poorly characterized. In the present study, the hepatotoxic substance carbon tetrachloride (CCl4) was employed to induce liver fibrosis in an animal model and agenome‑wide identification of lncRNAs in fibrotic liver tissues compared with CCl4 untreated liver tissues was performed using RNA sequencing...
May 7, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29743985/andrographolide-ameliorates-liver-fibrosis-in-mice-involvement-of-tlr4-nf-%C3%AE%C2%BA-b-and-tgf-%C3%AE-1-smad2-signaling-pathways
#16
Liteng Lin, Rui Li, Mingyue Cai, Jingjun Huang, Wensou Huang, Yongjian Guo, Liuhong Yang, Guizhi Yang, Tian Lan, Kangshun Zhu
Liver fibrosis is characterized by activated hepatic stellate cells (HSC) and extracellular matrix accumulation. Blocking the activation of HSC and the inflammation response are two major effective therapeutic strategies for liver fibrosis. In addition to the long history of using andrographolide (Andro) for inflammatory disorders, we aimed at elucidating the pharmacological effects and potential mechanism of Andro on liver fibrosis. In this study, liver fibrosis was induced by carbon tetrachloride (CCl4 ) and the mice were intraperitoneally injected with Andro for 6 weeks...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29742393/cxcl12-cxcr4-signaling-enhances-human-psc-derived-hematopoietic-progenitor-function-and-overcomes-early-in-vivo-transplantation-failure
#17
Jennifer C Reid, Borko Tanasijevic, Diana Golubeva, Allison L Boyd, Deanna P Porras, Tony J Collins, Mickie Bhatia
Human pluripotent stem cells (hPSCs) generate hematopoietic progenitor cells (HPCs) but fail to engraft xenograft models used to detect adult/somatic hematopoietic stem cells (HSCs) from donors. Recent progress to derive hPSC-derived HSCs has relied on cell-autonomous forced expression of transcription factors; however, the relationship of bone marrow to transplanted cells remains unknown. Here, we quantified a failure of hPSC-HPCs to survive even 24 hr post transplantation. Across several hPSC-HPC differentiation methodologies, we identified the lack of CXCR4 expression and function...
May 8, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29739329/thrombin-and-factor-xa-link-the-coagulation-system-with-liver-fibrosis
#18
Ameet Dhar, Fouzia Sadiq, Quentin M Anstee, Adam P Levene, Robert D Goldin, Mark R Thursz
BACKGROUND: Thrombin activates hepatic stellate cells via protease-activated receptor-1. The role of Factor Xa (FXa) in hepatic fibrosis has not been elucidated. We aimed to evaluate the impact of FXa and thrombin in vitro on stellate cells and their respective inhibition in vivo using a rodent model of hepatic fibrosis. METHODS: HSC-LX2 cells were incubated with FXa and/or thrombin in cell culture, stained for αSMA and relative gene expression and gel contraction calculated...
May 8, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29736022/adrenergic-nerve-degeneration-in-bone-marrow-drives-aging-of-the-hematopoietic-stem-cell-niche
#19
Maria Maryanovich, Ali H Zahalka, Halley Pierce, Sandra Pinho, Fumio Nakahara, Noboru Asada, Qiaozhi Wei, Xizhe Wang, Paul Ciero, Jianing Xu, Avigdor Leftin, Paul S Frenette
Aging of hematopoietic stem cells (HSCs) is associated with a decline in their regenerative capacity and multilineage differentiation potential, contributing to the development of blood disorders. The bone marrow microenvironment has recently been suggested to influence HSC aging, but the underlying mechanisms remain largely unknown. Here we show that HSC aging critically depends on bone marrow innervation by the sympathetic nervous system (SNS), as loss of SNS nerves or adrenoreceptor β3 signaling in the bone marrow microenvironment of young mice led to premature HSC aging, as evidenced by appearance of HSC phenotypes reminiscent of physiological aging...
May 7, 2018: Nature Medicine
https://www.readbyqxmd.com/read/29733056/asociaci%C3%A3-n-de-leptina-con-factores-cardiometab%C3%A3-licos-en-escolares-y-adolescentes-con-hiperplasia-suprarrenal-cong%C3%A3-nita
#20
Jessie Nallely Zurita-Cruz, Miguel Ángel Villasís-Keever, Leticia Damasio-Santana, Leticia Manuel-Apolinar, Rosalba Ferrusca-Ceja, Elisa Nishimura-Meguro, Aleida de J Rivera-Hernández, Eulalia Garrido-Magaña
Introducción: En la hiperplasia suprarrenal congénita (HSC), la obesidad, la hiperinsulinemia y los niveles de leptina se encuentran incrementados. Objetivo: Identificar la frecuencia de los factores de riesgo cardiometabólico (FRC) en niños y adolescentes con HSC y explorar la relación con los niveles de leptina. Método: Estudio transversal de 40 pacientes a quienes se realizó somatometría y evaluación de glucosa, insulina, triglicéridos, 17-hidroxiprogesterona, leptina, colesterol HDL y LDL en ayuno...
2018: Gaceta Médica de México
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