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https://www.readbyqxmd.com/read/29649281/classification-for-long-term-survival-in-oligometastatic-patients-treated-with-ablative-radiotherapy-a-multi-institutional-pooled-analysis
#1
Julian C Hong, Diandra N Ayala-Peacock, Jason Lee, A William Blackstock, Paul Okunieff, Max W Sung, Ralph R Weichselbaum, Johnny Kao, James J Urbanic, Michael T Milano, Steven J Chmura, Joseph K Salama
BACKGROUND: Radiotherapy is increasingly used to treat oligometastatic patients. We sought to identify prognostic criteria in oligometastatic patients undergoing definitive hypofractionated image-guided radiotherapy (HIGRT). METHODS: Exclusively extracranial oligometastatic patients treated with HIGRT were pooled. Characteristics including age, sex, primary tumor type, interval to metastatic diagnosis, number of treated metastases and organs, metastatic site, prior systemic therapy for primary tumor treatment, prior definitive metastasis-directed therapy, and systemic therapy for metastasis associated with overall survival (OS), progression-free survival (PFS), and treated metastasis control (TMC) were assessed by the Cox proportional hazards method...
2018: PloS One
https://www.readbyqxmd.com/read/29643758/prevalence-of-supplement-use-in-recreationally-active-kazakhstan-university-students
#2
Denis Vinnikov, Zhanna Romanova, Anar Dushpanova, Karashash Absatarova, Zhazira Utepbergenova
Background: Little is known about the supplements use and recreational sport practices in Kazakhstan university students. Therefore, the aim of this study was to ascertain supplements use prevalence and their predictors in this population. Methods: Cross-sectional survey of both undergraduate and graduate level students was completed in 2017 et al.-Farabi Kazakh National University, the largest higher institution in the country, from almost all Schools. A 45-item questionnaire was used to record physical activity, supplements use, lifestyle attributes (smoking, alcohol, sleep, etc...
2018: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/29642952/recombinase-polymerase-amplification-assay-combined-with-a-lateral-flow-dipstick-for-rapid-detection-of-tetracapsuloides-bryosalmonae-the-causative-agent-of-proliferative-kidney-disease-in-salmonids
#3
Hatem Soliman, Gokhlesh Kumar, Mansour El-Matbouli
BACKGROUND: The myxozoan Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease (PKD), is responsible for considerable losses in farmed and wild fish populations in Europe and North America. Recently, T. bryosalmonae was detected in many European countries, and strategy to control the disease in the wild and farmed fish population is yet to be developed. Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology that does not require any thermal cycling, and lateral flow dipstick (LFD) is a rapid, cost-effective, and easy-to-handle assay that enables stable detection...
April 11, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29642856/operational-utility-of-the-reverse-transcription-recombinase-polymerase-amplification-for-detection-of-dengue-virus
#4
Kim-Kee Tan, Noor Syahida Azizan, Che Norainon Yaacob, Nurul Asma Anati Che Mat Seri, Nur Izyan Samsudin, Boon-Teong Teoh, Sing-Sin Sam, Sazaly AbuBakar
BACKGROUND: A method for rapid detection of dengue virus using the reverse-transcription recombinase polymerase amplification (RT-RPA) was recently developed, evaluated and made ready for deployment. However, reliance solely on the evaluation performed by experienced researchers in a well-structured and well-equipped reference laboratory may overlook the potential intrinsic problems that may arise during deployment of the assay into new application sites, especially for users unfamiliar with the test...
April 11, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29630045/investigation-of-protein-recruitment-to-dna-lesions-using-405-nm-laser-micro-irradiation
#5
Antoine Gaudreau-Lapierre, Daniel Garneau, Billel Djerir, Frédéric Coulombe, Théo Morin, Alexandre Marechal
The DNA Damage Response (DDR) uses a plethora of proteins to detect, signal, and repair DNA lesions. Delineating this response is critical to understand genome maintenance mechanisms. Since recruitment and exchange of proteins at lesions are highly dynamic, their study requires the ability to generate DNA damage in a rapid and spatially-delimited manner. Here, we describe procedures to locally induce DNA damage in human cells using a commonly available laser-scanning confocal microscope equipped with a 405 nm laser line...
March 20, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29629556/aortic-re-implantation-in-a-case-of-anomalous-origin-of-the-left-coronary-artery-from-the-right-pulmonary-artery-in-a-toddler
#6
Vladlen V Bazylev, Igor E Chernogrivov, Aleksei E Chernogrivov
This tutorial  demonstrates correction of the rarest congenital coronary anomaly, anomalous origin of the left coronary artery from the right pulmonary artery (ALCAPA RPA). We present the features of surgical repair in the case of a toddler patient with an associated atrial septal defect.
February 26, 2018: Multimedia Manual of Cardiothoracic Surgery: MMCTS
https://www.readbyqxmd.com/read/29625920/simultaneous-detection-of-theileria-annulata-and-theileria-orientalis-infections-using-recombinase-polymerase-amplification
#7
Muhammad Adeel Hassan, Junlong Liu, Muhammad Sohail Sajid, Muhammad Rashid, Altaf Mahmood, Qamar Abbas, Guiquan Guan, Hong Yin, Jianxun Luo
Theileriosis is a disease of domesticated animals in tropical and subtropical countries and causes significant reductions in livestock productivity. The arid region of Punjab in Pakistan is notorious for the presence of the vector tick (Acari: Ixodidae) and tick-borne diseases, such as theileriosis and babesiosis. The distribution of Theileria annulata and T. orientalis in the Chakwal district of Punjab was determined by developing a multiplex recombinase polymerase amplification (RPA) assay as a scientific basis for formulating control strategies for bovine theileriosis...
March 28, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29621113/a-repeated-power-training-enhances-fatigue-resistance-while-reducing-intraset-fluctuations
#8
Oliver Gonzalo-Skok, Julio Tous-Fajardo, Gerard Moras, José Luis Arjol-Serrano, Alberto Mendez-Villanueva
Oliver, GS, Julio, TF, Moras, G, José Luis, AS, and Alberto, MV. A repeated power training enhances fatigue resistance while reducing intraset fluctuations. J Strength Cond Res XX(X): 000-000, 2018-The present study analyzed the effects of adding an upper-body repeated power ability (RPA) training to habitual strength training sessions. Twenty young elite male basketball players were randomly allocated into a control group (CON, n = 10) or repeated power group (RPG, n = 10) and evaluated by 1 repetition maximum (1RM), incremental load, and RPA tests in the bench press exercise before and after a 7-week period and a 4-week cessation period...
April 4, 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29619649/multi-center-study-finds-postoperative-residual-non-enhancing-component-of-glioblastoma-as-a-new-determinant-of-patient-outcome
#9
Aikaterini Kotrotsou, Ahmed Elakkad, Jia Sun, Ginu A Thomas, Dongni Yang, Srishti Abrol, Wei Wei, Jeffrey S Weinberg, Ali S Bakhtiari, Moritz F Kircher, Markus M Luedi, John F de Groot, Raymond Sawaya, Ashok J Kumar, Pascal O Zinn, Rivka R Colen
INTRODUCTION: The aim of the present study is to assess whether postoperative residual non-enhancing volume (PRNV) is correlated and predictive of overall survival (OS) in glioblastoma (GBM) patients. METHODS: We retrospectively analyzed a total 134 GBM patients obtained from The University of Texas MD Anderson Cancer Center (training cohort, n = 97) and The Cancer Genome Atlas (validation cohort, n = 37). All patients had undergone postoperative magnetic resonance imaging immediately after surgery...
April 4, 2018: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29618796/prognostic-value-of-cancer-antigen-125-for-lung-adenocarcinoma-patients-with-brain-metastasis-a-random-survival-forest-prognostic-model
#10
Hao Wang, Liuhai Shen, Jianhua Geng, Yitian Wu, Huan Xiao, Fan Zhang, Hongwei Si
Using random survival forest, this study was intended to evaluate the prognostic value of serum markers for lung adenocarcinoma patients with brain metastasis (BM), and tried to integrate them into a prognostic model. During 2010 to 2015, the patients were retrieved from two medical centers. Besides the Cox proportional hazards regression, the random survival forest (RSF) were also used to develop prognostic model from the group A (n = 142). In RSF of the group A, the factors, whose minimal depth were greater than the depth threshold or had a negative variable importance (VIMP), were firstly excluded...
April 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29606764/total-phenolic-content-and-total-antioxidant-capacity-of-common-indian-pulses-and-split-pulses
#11
Bhumi Parikh, V H Patel
Pulses are valued for their protein content and are good source of bioactive phytochemicals. The objectives of this study were to estimate total phenolic content (TPC), flavonoid content and total antioxidant capacity (TAC) through six different assay methods from twenty seven different varieties of pulses and split pulses commonly consumed in India. TPC and flavonoid content ranged between 38.6-542.7 mg GAE/100 g and 18.3-344.7 mg RE/100 g respectively. TAC measured by FRAP (90.6-2773.5 mg TE/100 g), RPA (190...
April 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29606045/tuberculum-sellae-meningiomas-grading-scale-to-assess-surgical-outcomes-using-the-transcranial-versus-transsphenoidal-approach
#12
Stephen T Magill, Ramin A Morshed, Calixto-Hope G Lucas, Manish K Aghi, Philip V Theodosopoulos, Mitchel S Berger, Oreste de Divitiis, Domenico Solari, Paolo Cappabianca, Luigi M Cavallo, Michael W McDermott
OBJECTIVE Tuberculum sellae meningiomas (TSMs) are surgically challenging tumors that can severely impair vision. Debate exists regarding whether the transcranial (TC) or endoscopic transsphenoidal (TS) approach is best for resecting these tumors, and there are few large series comparing these approaches. METHODS A retrospective chart review was performed at 2 academic centers comparing TC and TS approaches with respect to vision, extent of resection, recurrence, and complications. The authors report surgical outcomes and propose a simple preoperative tumor grading scale that scores tumor size (1-2), optic canal invasion (0-2), and arterial encasement (0-2)...
April 2018: Neurosurgical Focus
https://www.readbyqxmd.com/read/29602786/sensitive-and-specific-recombinase-polymerase-amplification-set-of-assays-for-fast-screening-detection-and-identification-of-bacillus-anthracis-in-a-field-setting
#13
Mostafa Bentahir, Jérôme Ambroise, Cathy Delcorps, Paola Pilo, Jean-Luc Gala
Four isothermal recombinase polymerase amplification assays (RPA) were developed for fast in-field identification of Bacillus (B.) anthracis RPAs targeted three specific sequences (i.e. BA_5345 chromosomal marker, lethal factor lef -pXO1, and capsule biosynthesis capA -pXO2), and a conserved sequence of the B. cereus group in the adenylate cyclase gene ( adk ). B. anthracis -specific RPA assays were first tested on purified genomic DNAs (n=60) containing 11 representatives of B. anthracis , then on soil (n=8) and white powder (n=8) samples spiked with inactivated B...
March 30, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29601890/the-silencing-of-replication-protein-a1-induced-cell-apoptosis-via-regulating-caspase-3
#14
Yuesheng Zhu, Yongdong Yi, Binglong Bai, Liyi Li, Tao You, Weijian Sun, Yaojun Yu
AIMS: Gastrointestinal cancers are a kind of deadly malignancy afflicting close to a million peoples worldwide. 5-Fluorouracil (5-Fu) is a main chemotherapeutic agent for cancer treatment. However, prolonged exposure of 5-Fu to cancer cells may cause chemoresistance and decrease the therapeutic potential of 5-Fu. MAIN METHODS: Replication protein A (RPA) is a component of the origin recognition complex. In our study, we explored the role of RPA1 in hepatocellular carcinoma cell SMMC-7721, gastric cancer cell SGC-7901 and colorectal cancer HT-29 via lentiviral particles infection...
March 27, 2018: Life Sciences
https://www.readbyqxmd.com/read/29599445/recruitment-kinetics-of-the-homologous-recombination-pathway-in-procyclic-forms-of-trypanosoma-brucei-after-ionizing-radiation-treatment
#15
Paula Andrea Marin, Marcelo Santos da Silva, Raphael Souza Pavani, Carlos Renato Machado, Maria Carolina Elias
One of the most important mechanisms for repairing double-strand breaks (DSBs) in model eukaryotes is homologous recombination (HR). Although the genes involved in HR have been found in Trypanosoma brucei and studies have identified some of the proteins that participate in this HR pathway, the recruitment kinetics of the HR machinery onto DNA during DSB repair have not been clearly elucidated in this organism. Using immunofluorescence, protein DNA-bound assays, and DNA content analysis, we established the recruitment kinetics of the HR pathway in response to the DSBs generated by ionizing radiation (IR) in procyclic forms of T...
March 29, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29598954/clinical-outcomes-and-prognostic-factors-in-cisplatin-versus-cetuximab-chemoradiation-for-locally-advanced-p16-positive-oropharyngeal-carcinoma
#16
Christian L Barney, Steve Walston, Pedro Zamora, Erin H Healy, Nicole Nolan, Virginia M Diavolitsis, Anterpreet Neki, Robert Rupert, Panos Savvides, Amit Agrawal, Matthew Old, Enver Ozer, Ricardo Carrau, Stephen Kang, James Rocco, Theodoros Teknos, John C Grecula, Jessica Wobb, Darrion Mitchell, Dukagjin Blakaj, Aashish D Bhatt
OBJECTIVES: Randomized trials evaluating cisplatin versus cetuximab chemoradiation (CRT) for p16+ oropharyngeal cancer (OPC) have yet to report preliminary data. Meanwhile, as a preemptive step toward morbidity reduction, the off-trial use of cetuximab in p16+ patients is increasing, even in those who could potentially tolerate cisplatin. The purpose of this study was to compare the efficacy of cisplatin versus cetuximab CRT in the treatment of p16+ OPC and to identify prognostic factors and predictors of tumor response...
April 2018: Oral Oncology
https://www.readbyqxmd.com/read/29590477/rna-splicing-factor-sart3-regulates-translesion-dna-synthesis
#17
Min Huang, Bo Zhou, Juanjuan Gong, Lingyu Xing, Xiaolu Ma, Fengli Wang, Wei Wu, Hongyan Shen, Chenyi Sun, Xuefei Zhu, Yeran Yang, Yazhou Sun, Yang Liu, Tie-Shan Tang, Caixia Guo
Translesion DNA synthesis (TLS) is one mode of DNA damage tolerance that uses specialized DNA polymerases to replicate damaged DNA. DNA polymerase η (Polη) is well known to facilitate TLS across ultraviolet (UV) irradiation and mutations in POLH are implicated in skin carcinogenesis. However, the basis for recruitment of Polη to stalled replication forks is not completely understood. In this study, we used an affinity purification approach to isolate a Polη-containing complex and have identified SART3, a pre-mRNA splicing factor, as a critical regulator to modulate the recruitment of Polη and its partner RAD18 after UV exposure...
March 24, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29577982/human-single-stranded-dna-binding-protein-1-hssb1-obfc2b-a-critical-component-of-the-dna-damage-response
#18
REVIEW
Laura V Croft, Emma Bolderson, Mark N Adams, Serene El-Kamand, Ruvini Kariawasam, Liza Cubeddu, Roland Gamsjaeger, Derek J Richard
Our genomic DNA is found predominantly in a double-stranded helical conformation. However, there are a number of cellular transactions and DNA damage events that result in the exposure of single stranded regions of DNA. DNA transactions require these regions of single stranded DNA, but they are only transient in nature as they are particularly susceptible to further damage through chemical and enzymatic degradation, metabolic activation, and formation of secondary structures. To protect these exposed regions of single stranded DNA, all living organisms have members of the Single Stranded DNA Binding (SSB) protein family, which are characterised by a conserved oligonucleotide/oligosaccharide-binding (OB) domain...
March 22, 2018: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/29576700/combined-antihypertensive-effect-of-paeoniflorin-enriched-extract-and-metoprolol-in-spontaneously-hypertensive-rats
#19
Bo Li, Zheng-Biao Yang, Shan-Sha Lei, Jie Su, Ze-Wu Jin, Su-Hong Chen, Gui-Yuan Lv
Background: Hypertension is a great global health challenge and it mostly requires drug combination therapy with the various advantages. Metoprolol (MP) and paeoniflorin are both commonly used for the treatment of hypertension. However, whether they exert synergistic effects on antihypertension or not remains unclear, especially on vascular endothelial function. Objective: The purpose of the study is to investigate the advantages of the combined antihypertensive effects of paeoniflorin enriched extract from Radix Paeoniae Alba (RE) and MP in spontaneously hypertensive rats (SHR)...
January 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29567868/-surgical-resection-of-metastatic-brain-tumors-risk-of-local-recurrence-and-leptomeningeal-dissemination-and-implications-for-individualized-treatment
#20
Yoko Nakasu, Koichi Mitsuya, Shoichi Deguchi, Nakamasa Hayashi, Hideyuki Harada
Metastatic brain tumors are important complications in the overall management of cancers. We sought to determine the risk of local recurrence and leptomeningeal carcinomatosis(LC)in patients treated with surgical resection and adjuvant radiation therapy. We retrospectively reviewed 173 consecutive patients with metastatic brain tumors managed by surgical resection between 2002 and 2015 in a single institution. Eighty-seven percent of the patients underwent postoperative adjuvant radiotherapy. The median overall survival from surgery was 9...
March 2018: No Shinkei Geka. Neurological Surgery
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