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https://www.readbyqxmd.com/read/28528216/prognostic-factors-in-patients-treated-with-surgery-for-brain-metastases-a-single-center-retrospective-analysis-of-125-patients
#1
Zhenghao Liu, Bingxi Lei, Meiguang Zheng, Zhongjun Li, Shuaibin Huang, Yuefei Deng
BACKGROUD: Brain metastases are the most common malignant intracranial tumors,however, the prognosis of patients is still poor despite multiple treatment have been applicated.The aim of this study was to analyse parameters influence overall survival from patient, tumor and treatment. Summarized characteristics of long-time(>2 years) survivors furtherly. MATERIALS AND METHODS: In total, clinical data of 125patients between 2004 and 2015 were collected and the parameters from patients, tumor and treatment were evaluated...
May 17, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28527442/inhomogeneous-fluid-of-penetrable-spheres-application-of-the-random-phase-approximation
#2
Yan Xiang, Derek Frydel
The focus of the present work is the application of the random phase approximation (RPA), derived for inhomogeneous fluids [Frydel and Ma, Phys. Rev. E 93, 062112 (2016)], to penetrable-spheres. As penetrable-spheres transform into hard-spheres with increasing interactions, they provide an interesting case for exploring the RPA, its shortcomings, and limitations, the weak- versus the strong-coupling limit. Two scenarios taken up by the present study are a one-component and a two-component fluid with symmetric interactions...
May 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28510991/development-of-a-panel-of-recombinase-polymerase-amplification-assays-for-detection-of-common-bacterial-urinary-tract-infection-pathogens
#3
Balakrishnan Raja, Heather J Goux, Archana Marapadaga, Sri Rajagopalan, Katerina Kourentzi, Richard C Willson
AIMS: To develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (RPA) assays for detection of common bacterial urinary tract infection (UTI) pathogens. METHODS AND RESULTS: The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Enterococcus faecalis. All five RPAs required reaction times of under 12 minutes to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with high concentrations of non-target bacterial genomic DNA...
May 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28510759/an-intact-mcm10-coiled-coil-interaction-surface-is-important-for-origin-melting-helicase-assembly-and-the-recruitment-of-pol-%C3%AE-to-mcm2-7
#4
Patricia Perez-Arnaiz, Irina Bruck, Max K Colbert, Daniel L Kaplan
Mcm10 is an essential eukaryotic factor required for DNA replication. The replication fork helicase is composed of Cdc45, Mcm2-7 and GINS (CMG). DDK is an S-phase-specific kinase required for replication initiation, and the DNA primase-polymerase in eukaryotes is pol α. Mcm10 forms oligomers in vitro, mediated by the coiled-coil domain at the N-terminal region of the protein. We characterized an Mcm10 mutant at the N-terminal Domain (NTD), Mcm10-4A, defective for self-interaction. We found that the Mcm10-4A mutant was defective for stimulating DDK phosphorylation of Mcm2, binding to eighty-nucleotide ssDNA, and recruiting pol α to Mcm2-7 in vitro...
May 16, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28505562/ultra-fast-electronic-detection-of-antimicrobial-resistance-genes-using-isothermal-amplification-and-thin-film-transistor-sensors
#5
Chunxiao Hu, Sumit Kalsi, Ioannis Zeimpekis, Kai Sun, Peter Ashburn, Carrie Turner, J Mark Sutton, Hywel Morgan
A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection...
May 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28500444/development-of-a-recombinase-polymerase-amplification-assay-for-the-diagnosis-of-banana-bunchy-top-virus-in-different-banana-cultivars
#6
Reetika Kapoor, Nishant Srivastava, Shailender Kumar, R K Saritha, Susheel Kumar Sharma, Rakesh Kumar Jain, Virendra Kumar Baranwal
Recombinase polymerase amplification (RPA) is a rapid, isothermal amplification method with high specificity and sensitivity. In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both in RPA as well as in polymerase chain reaction (PCR). A total of 133 symptomatic as well as asymptomatic banana leaf samples from various cultivars were collected from the different regions of India and evaluated for BBTV infection using the RPA assay...
May 12, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28484346/compare-the-performance-of-counselors-with-the-students-expectations-at-mazandaran-university-of-medical-sciences-in-2015
#7
Azita Balaghafari, Fattane Amuei, Nassim Ghahrani, Hasan Siamian
INTRODUCTION: Universities and academic environments are tackling challenges and transformation. Thus, mentally, spiritually and socially supporting students and academics and interacting with them seems necessary. This study aimed to compare the performance of counselors with the students' expectations at Mazandaran University of Medical Sciences in 2015. METHODS: In this cross-sectional study, 359 students of Mazandaran University of Medical Sciences were selected by using stratified random sampling...
March 2017: Materia Socio-medica
https://www.readbyqxmd.com/read/28483909/progerin-induced-replication-stress-facilitates-premature-senescence-in-hutchinson-gilford-progeria-syndrome
#8
Keith Wheaton, Denise Campuzano, Weili Ma, Michal Sheinis, Brandon Ho, Grant W Brown, Samuel Benchimol
Hutchinson-Gilford progeria syndrome (HGPS) is caused by a mutation in LMNA that produces an aberrant lamin A protein, progerin. The accumulation of progerin in HGPS cells leads to an aberrant nuclear morphology, genetic instability and p53-dependent premature senescence. How p53 is activated in response to progerin production is unknown. Here, we show that young, cycling HGPS fibroblasts, exhibit chronic DNA damage primarily in S phase as well as delayed replication fork progression. We demonstrate that progerin binds to PCNA altering its distribution away from replicating DNA in HGPS cells leading to γH2AX formation, ATR activation and RPA Ser33 phosphorylation...
May 8, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28475935/a-comparative-study-on-the-radioprotective-potential-of-the-polyphenolic-glycoconjugates-from-medicinal-plants-of-rosaceae-and-asteraceae-families-versus-their-aglycones
#9
Magdalena Szejk, Tomasz Poplawski, Anna Czubatka-Bienkowska, Alicja Klaudia Olejnik, Izabela Pawlaczyk-Graja, Roman Gancarz, Halina Malgorzata Zbikowska
Radioprotective potential of the polyphenolic glycoconjugates, isolated from flowers of Sanguisorba officinalis L. (So) and Erigeron canadensis L. (Ec), and from leaves of Fragaria vesca L. (Fv) and Rubus plicatus Whe. Et N. E. (Rp) as well as their aglycones (SoA, EcA, FvA and RpA, respectively), against γ-radiation-induced lipid peroxidation in human plasma and DNA damage in lymphocytes, were investigated in vitro. These properties were assessed by measuring the concentration of thiobarbituric acid reactive substances (TBARS) and using the alkaline comet assay, and were compared to the protective effects of rutin (R) and quercetin (Q)...
April 26, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28469045/effect-of-educational-program-based-on-precede-and-trans-theoretical-models-on-preventing-decline-in-regular-physical-activity-and-improving-it-among-students
#10
Masomeh Rostami-Moez, Forouzan Rezapur-Shahkolai, Seyyed Mohammad-Mahdi Hazavehei, Manoochehr Karami, Akram Karimi-Shahanjarini, Farzad Nazem
BACKGROUND: Adolescents especially middle school girls do not follow 60 min of regular physical activity (RPA), recommended by WHO, and endure physical activity decline. Using theory-based interventional program, considering the age of decline in RPA, seems to be effective. The aim of this study was to determine the effects of educational program, based on PRECEDE and Trans-Theoretical models, on preventing decline in RPA and improving it among 7th grade girl students. STUDY DESIGN: Randomized controlled trial...
April 10, 2017: Journal of Research in Health Sciences
https://www.readbyqxmd.com/read/28467896/cytoplasmic-atr-activation-promotes-vaccinia-virus-genome-replication
#11
Antonio Postigo, Amy E Ramsden, Michael Howell, Michael Way
In contrast to most DNA viruses, poxviruses replicate their genomes in the cytoplasm without host involvement. We find that vaccinia virus induces cytoplasmic activation of ATR early during infection, before genome uncoating, which is unexpected because ATR plays a fundamental nuclear role in maintaining host genome integrity. ATR, RPA, INTS7, and Chk1 are recruited to cytoplasmic DNA viral factories, suggesting canonical ATR pathway activation. Consistent with this, pharmacological and RNAi-mediated inhibition of canonical ATR signaling suppresses genome replication...
May 2, 2017: Cell Reports
https://www.readbyqxmd.com/read/28467467/dpb11-may-function-with-rpa-and-dna-to-initiate-dna-replication
#12
Irina Bruck, Nalini Dhingra, Matthew P Martinez, Daniel L Kaplan
Dpb11 is required for the initiation of DNA replication in budding yeast. We found that Dpb11 binds tightly to single-stranded DNA (ssDNA) or branched DNA structures, while its human homolog, TopBP1, binds tightly to branched-DNA structures. We also found that Dpb11 binds stably to CDK-phosphorylated RPA, the eukaryotic ssDNA binding protein, in the presence of branched DNA. A Dpb11 mutant specifically defective for DNA binding did not exhibit tight binding to RPA in the presence of DNA, suggesting that Dpb11-interaction with DNA may promote the recruitment of RPA to melted DNA...
2017: PloS One
https://www.readbyqxmd.com/read/28465576/paper-based-rna-detection-and-multiplexed-analysis-for-ebola-virus-diagnostics
#13
Laura Magro, Béatrice Jacquelin, Camille Escadafal, Pierre Garneret, Aurélia Kwasiborski, Jean-Claude Manuguerra, Fabrice Monti, Anavaj Sakuntabhai, Jessica Vanhomwegen, Pierre Lafaye, Patrick Tabeling
The most performing techniques enabling early diagnosis of infectious diseases rely on nucleic acid detection. Today, because of their high technicality and cost, nucleic acid amplification tests (NAAT) are of benefit only to a small fraction of developing countries population. By reducing costs, simplifying procedures and enabling multiplexing, paper microfluidics has the potential to considerably facilitate their accessibility. However, most of the studies performed in this area have not quit the lab. This letter brings NAAT on paper closer to the field, by using clinical samples and operating in a resource-limited setting...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28462945/select-men-benefit-from-androgen-deprivation-therapy-delivered-with-salvage-radiation-therapy-after-prostatectomy
#14
E B Holliday, D A Kuban, L B Levy, Y Bolukbasi, P Master, S Choi, Q Nguyen, S E McGuire, U Mahmood, S J Frank, K E Hoffman
BACKGROUND: Which men benefit most from adding androgen deprivation therapy (ADT) to salvage radiation therapy (SRT) after prostatectomy has not clearly been defined; therefore, we evaluated the impact of ADT to SRT on failure-free survival (FFS) in men with a rising or persistent PSA after prostatectomy. METHODS: We identified 332 men who received SRT after prostatectomy from 1987 to 2010. Recursive partitioning analysis (RPA) identified favorable, intermediate and unfavorable groups based on the risk of failure after SRT alone...
May 2, 2017: Prostate Cancer and Prostatic Diseases
https://www.readbyqxmd.com/read/28456788/a-prognostic-nomogram-for-overall-survival-after-neoadjuvant-radiotherapy-or-chemoradiotherapy-in-thoracic-esophageal-squamous-cell-carcinoma-a-retrospective-analysis
#15
Wei Deng, Qifeng Wang, Zefen Xiao, Lijun Tan, Zhao Yang, Zongmei Zhou, Hongxing Zhang, Dongfu Chen, Qinfu Feng, Jun Liang, Yexiong Li, Jie He, Shugeng Gao, Kelin Sun, Guiyu Cheng, Xiangyang Liu, Dekang Fang, Qi Xue, Yousheng Mao, Dali Wang, Jian Li
BACKGROUND: Currently, the AJCC staging system or pathological complete response (pCR) are considered not sufficiently accurate to evaluate the survival of patients with esophageal squamous cell carcinoma after neoadjuvant radiotherapy or chemoradiotherapy. This study aimed to establish a nomogram and a recursive partitioning analysis (RPA) model to estimate prognosis and to provide advice for subsequent treatments. METHODS: We analyzed retrospectively 407 patients that were diagnosed with thoracic esophageal squamous cell carcinoma (TESCC) and received neoadjuvant radiotherapy or chemoradiotherapy...
April 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28453612/meiosis-specific-proteins-meiob-and-spata22-cooperatively-associate-with-the-ssdna-binding-rpa-complex-and-dna-double-strand-breaks%C3%A2
#16
Yang Xu, Roger A Greenberg, Ernst Schonbrunn, P Jeremy Wang
Meiotic recombination ensures faithful segregation of homologous chromosomes during meiosis and generates genetic diversity in gametes. MEIOB, a meiosis-specific ssDNA-binding homologue of RPA1, is essential for meiotic recombination. Here we investigated the molecular mechanisms of MEIOB by characterizing its binding partners SPATA22 and RPA. We find that MEIOB and SPATA22 form an obligate complex and contain defined interaction domains. The interaction between these two proteins is unusual in that nearly any deletion in the binding domains abolishes the interaction...
April 27, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28431509/regular-recreational-physical-activity-and-risk-of-head-and-neck-cancer
#17
Chen-Lin Lin, Wei-Ting Lee, Chun-Yen Ou, Jenn-Ren Hsiao, Cheng-Chih Huang, Jehn-Shyun Huang, Tung-Yiu Wong, Ken-Chung Chen, Sen-Tien Tsai, Sheen-Yie Fang, Tze-Ta Huang, Jiunn-Liang Wu, Yuan-Hua Wu, Wei-Ting Hsueh, Chia-Jui Yen, Yu-Hsuan Lai, Hsiao-Chen Liao, Shang-Yin Wu, Ming-Wei Yang, Forn-Chia Lin, Jang-Yang Chang, Yi-Hui Wang, Ya-Ling Weng, Han-Chien Yang, Yu-Shan Chen, Jeffrey S Chang
BACKGROUND: Although substantial evidence supports a 20-30% risk reduction of colon cancer, breast cancer, and endometrial cancer by physical activity (PA), the evidence for head and neck cancer (HNC) is limited. Three published studies on the association between PA and HNC have generated inconsistent results. The current study examined the association between recreational PA (RPA) and HNC risk with a more detailed assessment on the intensity, frequency, duration, and total years of RPA...
April 21, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28430587/parkin-regulates-translesion-dna-synthesis-in-response-to-uv-radiation
#18
Xuefei Zhu, Xiaolu Ma, Yingfeng Tu, Min Huang, Hongmei Liu, Fengli Wang, Juanjuan Gong, Jiuqiang Wang, Xiaoling Li, Qian Chen, Hongyan Shen, Shu Zhu, Yun Wang, Yang Liu, Caixia Guo, Tie-Shan Tang
Deficiency of Parkin is a major cause of early-onset Parkinson's disease (PD). Notably, PD patients also exhibit a significantly higher risk in melanoma and other skin tumors, while the mechanism remains largely unknown. In this study, we show that depletion of Parkin causes compromised cell viability and genome stability after ultraviolet (UV) radiation. We demonstrate that Parkin promotes efficient Rad18-dependent proliferating cell nuclear antigen (PCNA) monoubiquitination by facilitating the formation of Replication protein A (RPA)-coated ssDNA upon UV radiation...
April 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28429132/development-of-a-real-time-recombinase-polymerase-amplification-assay-for-rapid-and-sensitive-detection-of-porcine-circovirus-2
#19
Jianchang Wang, Jinfeng Wang, Libing Liu, Wanzhe Yuan
Porcine diseases associated with porcine circovirus 2 (PCV-2) infection have resulted in significant economic losses worldwide. A real-time recombinase polymerase amplification (RPA) assay was developed to detect PCV-2 using primers and an exo probe specific for the ORF2 gene. The reaction process can be completed in 20 min at 38 °C. The assay only detects PCV-2, as there was no cross-reaction with other pathogens important in pigs. Using the PCV-2 genomic DNA as template, the analytical sensitivity of the real-time RPA was 103 copies...
April 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28424790/development-of-isothermal-recombinase-polymerase-amplification-assay-for-rapid-detection-of-porcine-circovirus-type-2
#20
Yang Yang, Xiaodong Qin, Yingjun Sun, Guozheng Cong, Yanmin Li, Zhidong Zhang
Porcine circovirus virus type II (PCV2) is the etiology of postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis, nephropathy syndrome (PDNS), and necrotizing pneumonia. Rapid diagnosis tool for detection of PCV2 plays an important role in the disease control and eradication program. Recombinase polymerase amplification (RPA) assays using a real-time fluorescent detection (PCV2 real-time RPA assay) and RPA combined with lateral flow dipstick (PCV2 RPA LFD assay) were developed targeting the PCV2 ORF2 gene...
2017: BioMed Research International
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