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https://www.readbyqxmd.com/read/29216299/recombinant-vaccinia-virus-coded-interferon-inhibitor-b18r-expression-refolding-and-a-use-in-a-mammalian-expression-system-with-a-rna-vector
#1
Yuriy G Kim, Aliya Zh Baltabekova, Erzhan E Zhiyenbay, Altynai S Aksambayeva, Zhadyra S Shagyrova, Rinat Khannanov, Erlan M Ramanculov, Alexandr V Shustov
B18R protein of Vaccinia virus binds to type I interferons and inhibits activation of interferon-mediated signal transduction. Cells which have unimpaired interferon signaling such as primary cell cultures or some industrially important cell lines are capable of development of an antiviral state. An establishment of the antiviral state limits replication of RNA-viruses and can suppress replication of RNA vectors. The interferon inhibitor B18R effectively prevents the establishment of the antiviral state. For this reason, B18R has become a ubiquitous component of protocols for epigenetic reprogramming which use transfections of RNA replicons or mRNA...
2017: PloS One
https://www.readbyqxmd.com/read/29213269/safety-and-immunogenicity-of-malaria-vectored-vaccines-given-with-routine-expanded-program-on-immunization-vaccines-in-gambian-infants-and-neonates-a-randomized-controlled-trial
#2
Victorine A Mensah, Sophie Roetynck, Ebrima K Kanteh, Georgina Bowyer, Amy Ndaw, Francis Oko, Carly M Bliss, Ya Jankey Jagne, Riccardo Cortese, Alfredo Nicosia, Rachel Roberts, Flavia D'Alessio, Odile Leroy, Babacar Faye, Beate Kampmann, Badara Cisse, Kalifa Bojang, Stephen Gerry, Nicola K Viebig, Alison M Lawrie, Ed Clarke, Egeruan B Imoukhuede, Katie J Ewer, Adrian V S Hill, Muhammed O Afolabi
Background: Heterologous prime-boost vaccination with chimpanzee adenovirus 63 (ChAd63) and modified vaccinia virus Ankara (MVA) encoding multiple epitope string thrombospondin-related adhesion protein (ME-TRAP) has shown acceptable safety and promising immunogenicity in African adult and pediatric populations. If licensed, this vaccine could be given to infants receiving routine childhood immunizations. We therefore evaluated responses to ChAd63 MVA ME-TRAP when co-administered with routine Expanded Program on Immunization (EPI) vaccines...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29209895/antiviral-activity-and-metal-ion-binding-properties-of-some-2-hydroxy-3-methoxyphenyl-acylhydrazones
#3
M Carcelli, E Fisicaro, C Compari, L Contardi, D Rogolino, C Solinas, A Stevaert, L Naesens
Here we report on the results obtained from an antiviral screening, including herpes simplex virus, vaccinia virus, vesicular stomatitis virus, Coxsackie B4 virus or respiratory syncytial virus, parainfluenza-3 virus, reovirus-1 and Punta Toro virus, of three 2-hydroxy-3-methoxyphenyl acylhydrazone compounds in three cell lines (i.e. human embryonic lung fibroblast cells, human cervix carcinoma cells, and African Green monkey kidney cells). Interesting antiviral EC50 values are obtained against herpes simplex virus-1 and vaccinia virus...
December 5, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29204136/suppression-of-poxvirus-replication-by-resveratrol
#4
Shuai Cao, Susan Realegeno, Anil Pant, Panayampalli S Satheshkumar, Zhilong Yang
Poxviruses continue to cause serious diseases even after eradication of the historically deadly infectious human disease, smallpox. Poxviruses are currently being developed as vaccine vectors and cancer therapeutic agents. Resveratrol is a natural polyphenol stilbenoid found in plants that has been shown to inhibit or enhance replication of a number of viruses, but the effect of resveratrol on poxvirus replication is unknown. In the present study, we found that resveratrol dramatically suppressed the replication of vaccinia virus (VACV), the prototypic member of poxviruses, in various cell types...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29203656/enigmatic-origin-of-the-poxvirus-membrane-from-the-endoplasmic-reticulum-shown-by-3d-imaging-of-vaccinia-virus-assembly-mutants
#5
Andrea S Weisberg, Liliana Maruri-Avidal, Himani Bisht, Bryan T Hansen, Cindi L Schwartz, Elizabeth R Fischer, Xiangzhi Meng, Yan Xiang, Bernard Moss
The long-standing inability to visualize connections between poxvirus membranes and cellular organelles has led to uncertainty regarding the origin of the viral membrane. Indeed, there has been speculation that viral membranes form de novo in cytoplasmic factories. Another possibility, that the connections are too short-lived to be captured by microscopy during a normal infection, motivated us to identify and characterize virus mutants that are arrested in assembly. Five conserved vaccinia virus proteins, referred to as Viral Membrane Assembly Proteins (VMAPs), that are necessary for formation of immature virions were found...
December 4, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29191985/reply-to-bioinformatics-analysis-of-differential-innate-immune-signaling-in-macrophages-by-wild-type-vaccinia-mature-virus-and-a-mutant-virus-with-a-deletion-of-the-a26-protein
#6
Siti Khadijah Kasani, Huei-Yin Cheng, Kun-Hai Eason Yeh, Shu-Jung Chang, Paul Wei-Che Hsu, Shu-Yun Tung, Chung-Tiang Liang, Wen Chang
No abstract text is available yet for this article.
December 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29189898/immunogenicity-of-recombinant-vaccinia-virus-vaccines-co-expressing-gp3-gp5-of-european-prrsv-and-cap-protein-of-pcv2-in-pigs
#7
Jicheng Han, Haibin Ma, Liang Cao, Jie Jing, Pengpeng Xiao, Wenchao Sun, Changzhan Xie, Shubo Wen, Yiquan Li, Mingyao Tian, Huijun Lu, Ningyi Jin
Porcine reproductive and respiratory syndrome (PRRS) is almost always caused by the North American strain of PRRS virus (PRRSV) in China; the European genotype of PRRSV has emerged in China. The mixed infection of PRRSV and Porcine circovirus type 2 virus (PCV2) are always found in pigs and PRRSV-augmented PCV2 replication and serious clinical symptoms. Current vaccines cannot protect mixed European PRRSV and PCV2 infections. Therefore, the development of a safe and effective new vaccine to prevent and control the mixed infection of European PRRSV and PCV2 is both urgent and necessary...
November 30, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29179469/bioluminescence-imaging-of-a-tumor-selective-thymidine-kinase-defective-vaccinia-virus-guang9-strain-after-intratumoral-or-intraperitoneal-administration-in-mice
#8
Yuedi Ding, Jun Fan, Lili Deng, Ying Peng, Jue Zhang, Biao Huang
Vaccinia virus has been used as an oncolytic virus because of its capacity to preferentially infect tumors rather than normal tissues. The vaccinia Tian Tan strain, used as a vaccine against smallpox for millions of people in China, is a promising candidate for cancer therapy. In this study, we constructed an attenuated Tian Tan strain of Guang9 with a disrupted thymidine kinase gene to enhance tumor selectivity and an inserted firefly luciferase to monitor the viral distribution by in vivo bioluminescence imaging...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29175625/increased-surface-expression-of-hiv-1-envelope-is-associated-with-improved-antibody-response-in-vaccinia-prime-protein-boost-immunization
#9
Michael J Hogan, Angela Conde-Motter, Andrea P O Jordan, Lifei Yang, Brad Cleveland, Wenjin Guo, Josephine Romano, Houping Ni, Norbert Pardi, Celia C LaBranche, David C Montefiori, Shiu-Lok Hu, James A Hoxie, Drew Weissman
HIV-1 envelope (Env)-based vaccines have so far largely failed to induce antibodies that prevent HIV-1 infection. One factor proposed to limit the immunogenicity of cell-associated Env is its low level of expression on the cell surface, restricting accessibility to antibodies. Using a vaccinia prime/protein boost protocol in mice, we explored the immunologic effects of mutations in the Env cytoplasmic tail (CT) that increased surface expression, including partial truncation and ablation of a tyrosine-dependent endocytosis motif...
November 22, 2017: Virology
https://www.readbyqxmd.com/read/29167527/the-effects-of-diets-enriched-in-omega-3-polyunsaturated-fatty-acids-on-systemic-vaccinia-virus-infection
#10
Gwendolyn J B Jones, Rachel L Roper
Omega-3 polyunsaturated fatty acids (PUFA, n-3 fatty acids), the key components of fish and flaxseed oils, are increasingly consumed by the public because of their potential health benefits and are available by prescription for hypertriglyceridemia. However, numerous studies have shown that these compounds are immunoregulatory and immunosuppressive and thus may increase susceptibility to infection. In this study, we tested the effects of the amount of fat and the types of fatty acid in the diet on infection by vaccinia virus, an acute infection that begins in the respiratory tract and spreads by viremia to internal organs...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29167334/flow-virometry-a-powerful-tool-to-functionally-characterize-viruses
#11
Roger Lippé
For several decades, flow cytometry has been a common approach to analyze cells and sort them to near purity. It enables one to probe inner cellular molecules, surface receptors or infected cells. However, the analysis of smaller entities such as viruses and exocytic vesicles has been more difficult but is becoming mainstream. This has in part been due to the development of new instrumentation with resolutions below conventional cytometers. It is also attributed to the several means to fluorescently label viruses, hence enabling them to stand out from the similarly sized background noise...
November 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29164070/generation-of-an-attenuated-tiantan-vaccinia-virus-strain-by-deletion-of-multiple-genes
#12
Yiquan Li, Yilong Zhu, Shuang Chen, Wenjie Li, Xunzhe Yin, Shanzhi Li, Pengpeng Xiao, Jicheng Han, Xiao Li, Lili Sun, Ningyi Jin
An attenuated vaccinia virus-MVTTEAB-was constructed by deletion of non-essential gene segments related to the immunomodulatory and virulence functions of the vaccinia virus Tiantan strain (VVTT). The shuttle plasmids pTC-EGFP, pTE-EGFP, pTA35-EGFP, pTB-EGFP, and pTA66-EGFP were constructed and combined with the early and late strong promoter pE/L and EGFP as an exogenous selectable marker. Then, through the homologous recombination technology and Cre/loxP system, the following gene fragments were gradually knocked out one by one: TC7L-TK2L, TE3L, TA35R, TB13R, and TA66R...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29159280/insertion-of-the-type-i-ifn-decoy-receptor-b18r-in-a-mirna-tagged-semliki-forest-virus-improves-oncolytic-capacity-but-results-in-neurotoxicity
#13
Tina Sarén, Mohanraj Ramachandran, Miika Martikainen, Di Yu
Oncolytic Semliki Forest virus (SFV) has been suggested as a potential candidate for the treatment of glioblastoma and neuroblastoma. However, the oncolytic capacity of SFV is restricted by the anti-viral type-I interferon (IFN) response. The aim of this study was to increase the oncolytic capacity of a microRNA target tagged SFV against glioblastoma by arming it with the Vaccinia-virus-encoded type-I IFN decoy receptor B18R (SFV4B18RmiRT) to neutralize type-I IFN response. Expression of B18R by SFV4B18RmiRT aided neutralization of IFN-β, which was shown by reduced STAT-1 phosphorylation and improved virus spread in plaque assays...
December 15, 2017: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/29158908/dairy-production-practices-and-associated-risks-for-bovine-vaccinia-exposure-in-cattle-brazil
#14
I A Borges, A M McCollum, J M Mehal, D Haberling, L A L Dutra, F N Vieira, L A O Andrade, E G Kroon, R C Holman, M G Reynolds, G S Trindade
A cross-sectional serosurvey was performed to identify environmental features or practices of dairy farms associated with risk for exposure to vaccinia-like viruses in dairy cattle in Brazil. Sera from 103 cows from 18 farms in Minas Gerais state were examined for Orthopoxvirus-neutralizing antibodies. A database of 243 binary or multiple-selection categorical variables regarding the physical features and surrounding ecology of each property was obtained. Thirteen of 46 presumptive predictor variables were found to be significantly associated with Orthopoxvirus serostatus by univariate logistic regression methods...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29140260/vaccinia-virus-natural-infections-in-brazil-the-good-the-bad-and-the-ugly
#15
REVIEW
Jaqueline Silva de Oliveira, Poliana de Oliveira Figueiredo, Galileu Barbosa Costa, Felipe Lopes de Assis, Betânia Paiva Drumond, Flávio Guimarães da Fonseca, Maurício Lacerda Nogueira, Erna Geessien Kroon, Giliane de Souza Trindade
The orthopoxviruses (OPV) comprise several emerging viruses with great importance to human and veterinary medicine, including vaccinia virus (VACV), which causes outbreaks of bovine vaccinia (BV) in South America. Historically, VACV is the most comprehensively studied virus, however, its origin and natural hosts remain unknown. VACV was the primary component of the smallpox vaccine, largely used during the smallpox eradication campaign. After smallpox was declared eradicated, the vaccination that conferred immunity to OPV was discontinued, favoring a new contingent of susceptible individuals to OPV...
November 15, 2017: Viruses
https://www.readbyqxmd.com/read/29137821/equination-inoculation-of-horsepox-an-early-alternative-to-vaccination-inoculation-of-cowpox-and-the-potential-role-of-horsepox-virus-in-the-origin-of-the-smallpox-vaccine
#16
REVIEW
José Esparza, Livia Schrick, Clarissa R Damaso, Andreas Nitsche
For almost 150 years after Edward Jenner had published the "Inquiry" in 1798, it was generally assumed that the cowpox virus was the vaccine against smallpox. It was not until 1939 when it was shown that vaccinia, the smallpox vaccine virus, was serologically related but different from the cowpox virus. In the absence of a known natural host, vaccinia has been considered to be a laboratory virus that may have originated from mutational or recombinational events involving cowpox virus, variola viruses or some unknown ancestral Orthopoxvirus...
November 11, 2017: Vaccine
https://www.readbyqxmd.com/read/29137298/ing4-expressing-oncolytic-vaccinia-virus-promotes-anti-tumor-efficiency-and-synergizes-with-gemcitabine-in-pancreatic-cancer
#17
Yinfang Wu, Xiaozhou Mou, Shibing Wang, Xing-E Liu, Xiaodong Sun
With no effective treatments available for most pancreatic cancer patients, pancreatic cancer continues to be one of the most difficult malignancies to treat. Oncolytic virus mediated-gene therapy has exhibited ubiquitous antitumor potential. In this study, we constructed a novel oncolytic vaccinia virus harboring the inhibitor of growth family member 4 gene (VV-ING4) to investigate its therapeutic efficacy alone or in combination with gemcitabine against pancreatic cancer cells in vitro and in vivo. ING4 expression was determined via quantitative real-time polymerase chain reaction (qPCR) and western blot...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29129932/the-vaccinia-virus-dna-polymerase-structure-provides-insights-into-the-mode-of-processivity-factor-binding
#18
Nicolas Tarbouriech, Corinne Ducournau, Stephanie Hutin, Philippe J Mas, Petr Man, Eric Forest, Darren J Hart, Christophe N Peyrefitte, Wim P Burmeister, Frédéric Iseni
Vaccinia virus (VACV), the prototype member of the Poxviridae, replicates in the cytoplasm of an infected cell. The catalytic subunit of the DNA polymerase E9 binds the heterodimeric processivity factor A20/D4 to form the functional polymerase holoenzyme. Here we present the crystal structure of full-length E9 at 2.7 Å resolution that permits identification of important poxvirus-specific structural insertions. One insertion in the palm domain interacts with C-terminal residues of A20 and thus serves as the processivity factor-binding site...
November 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/29123031/structure-function-characterization-of-three-human-antibodies-targeting-the-vaccinia-virus-adhesion-molecule-d8
#19
Michael H Matho, Andrew Schlossman, Iuliia M Gilchuk, Greg Miller, Zbigniew Mikulski, Matthias Hupfer, Jing Wang, Aruna Bitra, Xianghzi Meng, Yan Xiang, Tom Kaever, Tzanko Doukov, Klaus Ley, Shane Crotty, Bjoern Peters, Linda C Hsieh-Wilson, James E Crowe, Dirk M Zajonc
Vaccinia virus (VACV) envelope protein D8 is one of three glycosaminoglycan adhesion molecules and binds to the linear polysaccharide chondroitin sulfate (CS). D8 is also a target for neutralizing antibody responses that are elicited by the smallpox vaccine, which has enabled the first eradication of a human viral pathogen and is a useful model for studying antibody responses. However, to date, VACV epitopes targeted by human antibodies have not been characterized at atomic resolution. Here, we characterized the binding properties of several human anti-D8 antibodies and determined the crystal structures of three VACV-MAb variants, VACV-66, VACV-138, and VACV-304, separately bound to D8...
November 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29116169/a-zika-vaccine-targeting-ns1-protein-protects-immunocompetent-adult-mice-in-a-lethal-challenge-model
#20
Aaron C Brault, Arban Domi, Erin M McDonald, Dalit Talmi-Frank, Nathanael McCurley, Rahul Basu, Harriet L Robinson, Michael Hellerstein, Nisha K Duggal, Richard A Bowen, Farshad Guirakhoo
Zika virus (ZIKV) is a mosquito-borne flavivirus that has rapidly extended its geographic range around the world. Its association with abnormal fetal brain development, sexual transmission, and lack of a preventive vaccine have constituted a global health concern. Designing a safe and effective vaccine requires significant caution due to overlapping geographical distribution of ZIKV with dengue virus (DENV) and other flaviviruses, possibly resulting in more severe disease manifestations in flavivirus immune vaccinees such as Antibody-Dependent Enhancement (ADE, a phenomenon involved in pathogenesis of DENV, and a risk associated with ZIKV vaccines using the envelope proteins as immunogens)...
November 7, 2017: Scientific Reports
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