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https://www.readbyqxmd.com/read/29038898/molecular-and-immunological-characterisation-of-tropomyosin-from-anisakis-pegreffii
#1
Abdouslam Asnoussi, Ibukun E Aibinu, Robin B Gasser, Andreas L Lopata, Peter M Smooker
Tropomyosin (TM) is a major allergen in shellfish, known to cross-react with mite, cockroach and/or some roundworm (nematode) TM. In this study, we aimed to express and purify TM from the parasitic nematode Anisakis pegreffii and also to characterise its cross-reactivity with TM from shellfish. A. pegreffii was isolated from the flathead tiger fish (Neoplatycephalus richardsoni) and characterised using single-strand conformation polymorphism (SSCP)-based sequencing of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA...
October 16, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29030997/rna-interference-and-functional-characterization-of-a-tergal-gland-alpha-amylase-in-the-german-cockroach-blattella-germanica-l
#2
A J Myers, A D Gondhalekar, M Fardisi, K D Pluchar, K D Saltzmann, G W Bennett, M E Scharf
German cockroach males possess tergal glands that secrete a combination of oligosaccharides, lipids and proteins. Four major proteins occur in the secretion, with one being the 63 kDa alpha-amylase Blattella germanica Tergal Gland protein-1 (BGTG-1). Denaturing and starch gel electrophoresis coupled with peptide sequencing verified amylase activity for the BGTG-1 protein. BGTG-1 gene expression profiles were determined by using quantitative real-time PCR to compare messenger RNA abundance among isolated tissues of males, females and gravid females...
October 14, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29029107/new-food-baits-for-trapping-german-cockroaches-blattella-germanica-l-dictyoptera-blattellidae
#3
Joshua C Pol, Sebastian Ibarra Jimenez, Gerhard Gries
German cockroaches (GCRs), Blattella germanica (L.) (Dictyoptera: Blattellidae), are attracted to those beer semiochemicals (e.g., ethanol) that formerly living and active yeasts have produced or otherwise formed in the brewing process. We predicted that an earlier step in the production of beer, where yeasts actively metabolize the sugar in malted barley powder (dry malt extract [DME]), is very attractive to GCRs. In laboratory experiments, a 3-component composition (3CC) comprising DME, water, and Brewer's yeast strongly attracted GCR nymphs, females, and males...
September 27, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29029091/cockroach-oothecal-parasitoid-evania-appendigaster-hymenoptera-evaniidae-exhibits-oviposition-preference-towards-oothecal-age-most-vulnerable-to-host-cannibalism
#4
Hui-Siang Tee, Chow-Yang Lee
Many female parasitoid wasps optimize host selection to balance the benefits of high-quality hosts and the costs of predator- or hyperparasitoid-induced mortality risks to maximize their fitness. Cannibalism exists in many insect species and affects survival of parasitoid larvae developing in or on parasitized hosts. However, little is known about how parasitoid wasps resolve the fitness consequence of host cannibalism-induced mortality risk during host selection. We examined the effect of oothecal age on cannibalism in the American cockroach Periplaneta americana (L...
September 27, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29020923/comparative-analysis-of-mirna-expression-during-the-development-of-insects-of-different-metamorphosis-modes-and-germ-band-types
#5
Guillem Ylla, Maria-Dolors Piulachs, Xavier Belles
BACKGROUND: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. RESULTS: We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined...
October 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28990095/kangfuxin-promotes-apoptosis-of-gastric-cancer-cells-through-activating-er%C3%A2-stress-and-autophagy
#6
Pian-Pian Chen, Xiu-Ying Ma, Qian Lin, He-Lin Xu, Hong-Xue Shi, Hong-Yu Zhang, Jian Xiao, Fu-Neng Geng, Ying-Zheng Zhao
Gastric cancer is a leading cause of cancer‑associated mortality worldwide. In studies on the mechanisms of antigastric cancer drugs, autophagy and endoplasmic reticulum (ER) stress have been demonstrated to serve an active role in gastric cancer. The organic extract of Periplaneta americana (also termed American Cockroach), which is named Kangfuxin (KFX) in China, has been used clinically as a traditional Chinese medicine against disorders, including stomach bleeding, gastric ulcers, tuberculosis, burns and trauma...
October 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28986693/the-prevalence-of-protozoa-in-the-gut-of-german-cockroaches-blattella-germanica-with-special-reference-to-lophomonas-blattarum
#7
Rafael Martínez-Girón, Cristina Martínez-Torre, Hugo Cornelis van Woerden
The German cockroach (Blattella germanica) is a common domestic pest, which produces allergens that have been associated with broncho-pulmonary disease. Various protozoan species have been identified in the intestine of this cockroach and it has been hypothesised that these protozoa, or their proteases, may contribute to the burden of cockroach-associated allergens and adjuvants present in domestic dust. The aim of this study was therefore to determine the prevalence of protozoan species in the intestine of Blattella germanica...
October 7, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28974756/serum-zinc-status-and-its-association-with-allergic-sensitization-the-fifth-korea-national-health-and-nutrition-examination-survey
#8
Hyun-Min Seo, Yeong Ho Kim, Ji Hyun Lee, Joung Soo Kim, Young Min Park, Jun Young Lee
Zinc (Zn) is an essential trace element that plays important roles in the immune system. There is little known about the role of trace elements in allergic diseases, and previous reports have shown conflicting results. The aim of this study was to investigate the relationship between serum Zn levels and total or allergen-specific immunoglobulin E (IgE) levels. The initial candidates for this study were those who participated in the 5(th) Korean National Health and Nutrition Examination Survey 2010 (n = 8,958), and 1,867 adults who had serum total and allergen specific-IgE levels measured were included...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28973021/pycnoscelus-surinamensis-cockroach-gut-microbiota-respond-consistently-to-a-fungal-diet-without-mirroring-those-of-fungus-farming-termites
#9
Callum Richards, Saria Otani, Aram Mikaelyan, Michael Poulsen
The gut microbiotas of cockroaches and termites play important roles in the symbiotic digestion of dietary components, such as lignocellulose. Diet has been proposed as a primary determinant of community structure within the gut, acting as a selection force to shape the diversity observed within this "bioreactor", and as a key factor for the divergence of the termite gut microbiota from the omnivorous cockroach ancestor. The gut microbiota in most termites supports primarily the breakdown of lignocellulose, but the fungus-farming sub-family of higher termites has become similar in gut microbiota to the ancestral omnivorous cockroaches...
2017: PloS One
https://www.readbyqxmd.com/read/28969780/experimental-transmission-of-toxocara-canis-from-blattella-germanica-and-periplaneta-americana-cockroaches-to-a-paratenic-host
#10
T González-García, M A Muñoz-Guzmán, H Sánchez-Arroyo, M G Prado-Ochoa, J A Cuéllar-Ordaz, F Alba-Hurtado
The present study assessed the capacity of Blattella germanica and Periplaneta americana to disseminate and transmit infective phases of T. canis to rats, which were used as a model paratenic host. P. americana and B. germanica inoculated orally with T. canis larvated eggs shed eggs and larvae in their fecal matter during the first 6days post-inoculation. Larvae were recovered from the brain, lungs, kidneys and liver of rats that had been inoculated with either infected cockroaches or their feces. ELISAs of serum detected an increase of antibodies anti-T...
November 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28967211/novel-use-of-aliphatic-n-methyl-ketones-as-a-fumigant-and-alternative-to-methyl-bromide-for-insect-control
#11
Jiwei Zhu, Anirudh Dhammi, Jaap B van Kretschmar, Edward L Vargo, Charles S Apperson, R Michael Roe
BACKGROUND: Fumigants like phosphine, methyl bromide and sulfuryl fluoride are highly effective for the control of structural, storage and agricultural arthropod pests. Unfortunately, many of these synthetic compounds are highly toxic to people, many pests have developed resistance to these compounds, and methyl bromide, the "gold standard" for fumigants, was de-registered because of its depletion of the stratospheric ozone layer. Alternative fumigant chemistry is needed. RESULTS: Several plant species produce n-aliphatic methyl ketones to prevent plant herbivory...
October 2, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28962920/allergy-and-immunology-in-africa-challenges-and-unmet-needs
#12
Yehia M El-Gamal, Elham M Hossny, Zeinab A El-Sayed, Shereen M Reda
The tremendous increase in allergy in the African continent cannot simply be explained by the change in public hygiene. There are many "prehygiene" communities with sewage-contaminated water supplies, helminth infestations, bare footedness, and poor housing, and still there is a high prevalence of allergic disease. Africans can be exposed to many risk factors facilitating severe asthma and wheezing, including airborne viruses, smoke, indoor dampness, cockroaches, and poor access to health care. Although the reporting on food allergy is inadequate to perform systematic reviews or meta-analyses, the available data suggest that food allergy is underdiagnosed...
September 26, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28955424/first-aid-practices-beliefs-and-sources-of-information-among-caregivers-regarding-paediatric-burn-injuries-in-harare-zimbabwe-a-cross-sectional-study
#13
Farai Chirongoma, Samson Chengetanai, Cathrine Tadyanemhandu
BACKGROUND: While burns take seconds to occur, injuries incurred result in pain and undesirable long term effects that might take a lifetime to overcome. The study was carried out to determine the measures of first aid delivered by caregivers after a burn injury and sources of the information. METHODS: A cross- sectional study was carried out over a period of 3 months at two central hospitals in Harare. A questionnaire was administered to the caregivers of children within the age group of 0-60 months admitted in burns wards to elicit information on the circumstances of the burn injury and the first aid methods which were administered...
June 2017: Malawi Medical Journal: the Journal of Medical Association of Malawi
https://www.readbyqxmd.com/read/28949065/the-american-cockroach-peptide-periplanetasin-4-inhibits-clostridium-difficile-toxin-a-induced-cell-toxicities-and-inflammatory-responses-in-the-mouse-gut
#14
I Na Yoon, Li Fang Lu, Ji Hong, Peng Zhang, Dae Hong Kim, Jin Ku Kang, Jae Sam Hwang, Ho Kim
Many reports have shown that crude extracts of the American cockroach have therapeutic effects on inflammation. In a previous study, our research group showed that an antimicrobial peptide (Periplanetasin-2) derived from the American cockroach via de novo transcriptome analysis inhibited apoptosis of human colonocytes and inflammatory responses of the mouse gut caused by Clostridium difficile toxin A. Here, we examined whether Periplanetasin-4 (Peri-4), another antimicrobial peptide identified via de novo transcriptome analysis of the American cockroach, could also inhibit the various toxicities induced by C...
September 26, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28947081/chronic-il-33-expression-predisposes-to-viral-induced-exacerbations-of-asthma-by-increasing-type-2-inflammation-and-dampening-antiviral-immunity
#15
Rhiannon B Werder, B Biomed Sci, Vivian Zhang, Jason P Lynch, Natale Snape, John W Upham, Kirsten Spann, Simon Phipps
BACKGROUND: Rhinovirus infection triggers acute exacerbations of asthma. IL-33 is an instructive cytokine of type-2 inflammation whose expression is associated with viral load during experimental rhinovirus infection of asthmatic subjects. OBJECTIVE: To determine whether anti-IL-33 therapy is effective during disease progression, established disease, or viral exacerbation using a preclinical model of chronic asthma and in vivo human primary airway epithelial cells (AECs)...
September 22, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28944036/time-lapse-camera-trapping-as-an-alternative-to-pitfall-trapping-for-estimating-activity-of-leaf-litter-arthropods
#16
Rachael A Collett, Diana O Fisher
Pitfall trapping is the standard technique to estimate activity and relative abundance of leaf litter arthropods. Pitfall trapping is not ideal for long-term sampling because it is lethal, labor-intensive, and may have taxonomic sampling biases. We test an alternative sampling method that can be left in place for several months at a time: verticallyplaced time-lapse camera traps that have a short focal distance, enabling identification of small arthropods. We tested the effectiveness of these time-lapse cameras, and quantified escape and avoidance behavior of arthropod orders encountering pitfall traps by placing cameras programed with a range of sampling intervals above pitfalls, to assess numerical, taxonomic, and body size differences in samples collected by the two methods...
September 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28942048/the-cockroach-periplaneta-americana-as-a-potential-paratenic-host-of-the-lungworm-aelurostrongylus-abstrusus
#17
Luigi Falsone, Vito Colella, Ettore Napoli, Emanuele Brianti, Domenico Otranto
INTRODUCTION: Aelurostrongylus abstrusus is a well-known nematode affecting the respiratory system of felids worldwide. Snails and slugs act as intermediate hosts of this parasite, whereas rodents, birds and reptiles may serve as paratenic hosts. Periplaneta americana, the American brown cockroach, shares the same habitat and ecological features (e.g. nocturnal activity) with both snails and cats. The aim of this study was to evaluate the capability of P. americana to maintain alive A...
September 21, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28940559/proteinase-activated-receptor-2-blockade-inhibits-changes-seen-in-a-chronic-murine-asthma-model
#18
M Asaduzzaman, C Davidson, D Nahirney, Y Fiteih, L Puttagunta, H Vliagoftis
BACKGROUND: Proteinase-Activated Receptor-2 (PAR2 ) is a G protein-coupled receptor activated by serine proteinases. We have shown that PAR2 activation in the airways is involved in the development of allergic inflammation and airway hyperresponsiveness (AHR) in acute murine models. We hypothesized that functional inhibition of PAR2 prevents allergic inflammation, AHR and airway remodeling in chronic allergic airway inflammation models. MATERIAL AND METHODS: We developed and used a 12 week model of cockroach extract (CE)-mediated AHR, airway inflammation and remodeling in BALB/c mice...
September 20, 2017: Allergy
https://www.readbyqxmd.com/read/28939248/early-life-home-environment-and-risk-of-asthma-among-inner-city-children
#19
George T O'Connor, Susan V Lynch, Gordon R Bloomberg, Meyer Kattan, Robert A Wood, Peter J Gergen, Katy F Jaffee, Agustin Calatroni, Leonard B Bacharier, Avrahman Beigelman, Megan T Sandel, Christine C Johnson, Ali Faruqi, Clark Santee, Kei E Fujimura, Douglas Fadrosh, Homer Boushey, Cynthia M Visness, James E Gern
BACKGROUND: Environmental exposures in early life appear to play an important role in the pathogenesis of childhood asthma, but the potentially modifiable exposures that lead to asthma remain uncertain. OBJECTIVE: We sought to identify early-life environmental risk factors for childhood asthma in a birth cohort of high-risk inner-city children. METHODS: We examined the relationship of prenatal and early-life environmental factors to the occurrence of asthma at 7 years of age among 442 children...
September 12, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28934335/genetic-diversity-of-entamoeba-novel-ribosomal-lineages-from-cockroaches
#20
Tetsuro Kawano, Mihoko Imada, Pennapa Chamavit, Seiki Kobayashi, Tetsuo Hashimoto, Tomoyoshi Nozaki
Our current taxonomic perspective on Entamoeba is largely based on small-subunit ribosomal RNA genes (SSU rDNA) from Entamoeba species identified in vertebrate hosts with minor exceptions such as E. moshkovskii from sewage water and E. marina from marine sediment. Other Entamoeba species have also been morphologically identified and described from non-vertebrate species such as insects; however, their genetic diversity remains unknown. In order to further disclose the diversity of the genus, we investigated Entamoeba spp...
2017: PloS One
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