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https://www.readbyqxmd.com/read/29775789/e-coli-encoding-blandm-5-associated-with-community-acquired-uti-cases-with-unusual-mic-creep-like-phenomenon-against-imipenem
#1
Varsha Rani Gajamer, Amitabha Bhattacharjee, Deepjyoti Paul, Chandrayee Deshamukhya, Ashish Kr Singh, Nilu Pradhan, Hare Krishna Tiwari
OBJECTIVES: Carbapenemase-producing Escherichia coli are of major clinical concern. The present study aimed to identify NDM-5 producing E.coli associated with community-acquired urinary tract infection, co-harboring ESBL genes and a pattern of imipenem MIC creep. METHODS: A total of 973 urine samples were collected from females of age between 18-49 diagnosed with UTI. Isolates were identified by standard microbiological procedures. Presence of blaNDM and ESBL genes was determined by PCR assay and sequencing...
May 15, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29773850/comparative-genomic-analysis-of-acinetobacter-spp-plasmids-originating-from-clinical-settings-and-environmental-habitats
#2
Ileana P Salto, Gonzalo Torres Tejerizo, Daniel Wibberg, Alfred Pühler, Andreas Schlüter, Mariano Pistorio
Bacteria belonging to the genus Acinetobacter have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among Acinetobacter strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental Acinetobacter spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29773634/complete-genome-sequence-of-ochrobactrum-haematophilum-fi11154-isolated-from-kunu-zaki-a-nigerian-millet-based-fermented-food
#3
Maria Diaz, Udo Wegmann, Nwanneka Akinyemi, Folarin A Oguntoyinbo, Lizbeth Sayavedra, Melinda J Mayer, Arjan Narbad
Ochrobactrum haematophilum FI11154 was isolated from kunu-zaki, a Nigerian traditional fermented millet-based food. Here, we present the first complete genome sequence of this species. The genome consists of five replicons and contains genes related to iron uptake and phosphatase activities.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29762480/time-course-microarray-analysis-reveals-differences-between-transcriptional-changes-in-tomato-leaves-triggered-by-mild-and-severe-variants-of-potato-spindle-tuber-viroid
#4
Aneta Więsyk, Roksana Iwanicka-Nowicka, Anna Fogtman, Włodzimierz Zagórski-Ostoja, Anna Góra-Sochacka
Viroids are small non-capsidated non-coding RNA replicons that utilize host factors for efficient propagation and spread through the entire plant. They can incite specific disease symptoms in susceptible plants. To better understand viroid-plant interactions, we employed microarray analysis to observe the changes of gene expression in "Rutgers" tomato leaves in response to the mild (M) and severe (S23) variants of potato spindle tuber viroid (PSTVd). The changes were analyzed over a time course of viroid infection development: (i) the pre-symptomatic stage; (ii) early symptoms; (iii) full spectrum of symptoms and (iv) the so-called 'recovery' stage, when stem regrowth was observed in severely affected plants...
May 15, 2018: Viruses
https://www.readbyqxmd.com/read/29753657/irf-1-rig-i-and-mda5-display-potent-antiviral-activities-against-norovirus-coordinately-induced-by-different-types-of-interferons
#5
Wen Dang, Lei Xu, Yuebang Yin, Sunrui Chen, Wenshi Wang, Mohamad S Hakim, Kyeong-Ok Chang, Maikel P Peppelenbosch, Qiuwei Pan
Norovirus represents the main cause of acute nonbacterial gastroenteritis worldwide. In immunocompromised patients, it bears high risk of causing chronic infection with significant morbidity and mortality. The lack of specific treatment prompts the development of anti-norovirus agents. In this study, we have investigated the role of interferon (IFN) response and evaluated antiviral activities of different IFNs against human norovirus (HuNoV) replication using a HuNoV replicon model. We found that HuNoV RNA replication was sensitive to all types of IFNs, including IFNα (type I), IFNγ (type II), IFNλ1 and 3 (type III)...
May 10, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29749134/mir-215-enhances-hcv-replication-by-targeting-trim22-and-inactivating-nf-%C3%AE%C2%BAb-signaling
#6
Hui Tian, Zhenkun He
PURPOSE: Hepatitis C virus (HCV) infection is a major cause of liver disease. Several miRNAs have been found to be associated with HCV infection. This study aimed to investigate the functional roles and possible molecular mechanisms of miR-215 in HCV replication. MATERIALS AND METHODS: The expression levels of miR-215 and TRIM22 were detected by quantitative real-time PCR (qRT-PCR) and western blot analysis in Con1b subgenomic genotype 1b HCV replicon cells (Con1b cells) and JFH1 full genome infecting Huh7...
June 2018: Yonsei Medical Journal
https://www.readbyqxmd.com/read/29744043/genome-based-analysis-of-carbapenemase-producing-klebsiella-pneumoniae-isolates-from-german-hospital-patients-2008-2014
#7
Laura Becker, Martin Kaase, Yvonne Pfeifer, Stephan Fuchs, Annicka Reuss, Anja von Laer, Muna Abu Sin, Miriam Korte-Berwanger, Sören Gatermann, Guido Werner
Background: By using whole genome sequence data we aimed at describing a population snapshot of carbapenemase-producing K. pneumoniae isolated from hospitalized patients in Germany between 2008 and 2014. Methods: We selected a representative subset of 107 carbapenemase-producing K. pneumoniae clinical isolates possessing the four most prevalent carbapenemase types in Germany (KPC-2, KPC-3, OXA-48, NDM-1). Isolates were processed via illumina NGS. Data were analysed using different SNP-based mapping and de-novo assembly approaches...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29727717/coexistence-of-ndm-1-producing-escherichia-coli-and-citrobacter-freundii-in-the-same-patient
#8
Bo Zhu, Chaoqun Ying, Hao Xu, Jingjing Ying
OBJECTIVES: Although blaNDM-1 has now been widely detected in various Enterobacteriaceae clinical isolates, multiple blaNDM-1 colonizations within the same patient remain rare. The aim of the study was to describe a patient with respiratory tract colonization of NDM-1-producing Escherichia coli and Citrobacter freundii during a hospitalization in China. METHODS: Two carbapenem-resistant isolates were analysed. Susceptibility testing, metallo-β-lactamase production, conjugation, plasmid analysis, and molecular typing were performed...
May 1, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29724185/clonal-diversity-and-genetic-profiling-of-antibiotic-resistance-among-multidrug-carbapenem-resistant-klebsiella-pneumoniae-isolates-from-a-tertiary-care-hospital-in-saudi-arabia
#9
Taher Uz Zaman, Maha Alrodayyan, Maha Albladi, Mohammed Aldrees, Mohammed Ismail Siddique, Sameera Aljohani, Hanan H Balkhy
BACKGROUND: The nexus between resistance determinants, plasmid type, and clonality appears to play a crucial role in the dissemination and survival of carbapenem-resistant Klebsiella pneumoniae (CRKP). The incidence of infections involving CRKP in Saudi Arabia is increasing and there is a need for detailed molecular profiling of this pathogen for CRKP surveillance and control. METHODS: The resistance determinants of 71 non-redundant CRKP isolates were investigated by polymerase chain reaction (PCR) and sequencing...
May 3, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29718575/roles-of-the-genomic-sequence-surrounding-the-stem-loop-structure-in-the-junction-region-including-the-3-terminus-of-orf1-in-hepatitis-e-virus-replication
#10
Dianjun Cao, Yan-Yan Ni, Michelle Walker, Yao-Wei Huang, Xiang-Jin Meng
Hepatitis E virus (HEV), a member of the family Hepeviridae, causes both acute and chronic viral hepatitis. We have previously demonstrated that the stem-loop structure in the junction region (JR) of HEV genome plays a critical role in HEV replication. However, the function of the sequence bordering the JR, including the 3' terminus of ORF1, in HEV replication is unknown. In this study, a panel of HEV Renilla luciferase (Rluc) replicons containing various deletions at 5' or 3' termini of the JR were constructed to determine the effect of the deletions on HEV replication in Huh7 human liver cells...
May 2, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29715528/functional-analysis-of-duck-goose-and-ostrich-2-5-oligoadenylate-synthetase
#11
Hassan T Tag-El-Din-Hassan, Masami Morimatsu, Takashi Agui
Up-to-date the flavivirus infection in avian taxa is not clearly defined. Several reports have demonstrated that many viruses belonging to Flaviviridae may cause diseases in poultry species; however, the susceptibility of other avian species is variable and still unclear. In human and mice, the 2'-5'-oligoadenylate synthetase (OAS) proteins are associated with resistance to the flavivirus infection as well as other virus infections. However, the avian OAS proteins are rarely studied. In our previous studies, we confirmed that the chicken OAS-like protein (chOASL) expressed OAS-enzymatic activity (the classical OAS/RNase L-dependent pathway) as well as the anti-flavivirus activity (the putative OAS/RNase L-independent pathway)...
April 28, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29705238/hcv-modifies-egf-signalling-and-upregulates-production-of-cxcr2-ligands-role-in-inflammation-and-antiviral-immune-response
#12
Christina Groepper, Kerstin Rufinatscha, Nadja Schröder, Sabine Stindt, Christian Ehlting, Ute Albrecht, Hans H Bock, Ralf Bartenschlager, Dieter Häussinger, Johannes G Bode
BACKGROUND & AIMS: To affect immune response and inflammation, the hepatitis C virus (HCV) substantially influences intercellular communication pathways that are decisive for immune cell recruitment. The present study investigates mechanisms by which HCV modulates chemokine-mediated intercellular communication of the infected cells. METHODS: Chemokine expression is studied in HCVcc -infected cell lines or cell lines harbouring a subgenomic replicon as well as in serum samples from patients...
April 26, 2018: Journal of Hepatology
https://www.readbyqxmd.com/read/29698126/whole-genome-sequencing-of-ndm-1-producing-st11-klebsiella-pneumoniae-isolated-in-a-private-laboratory-in-greece
#13
Georgios Meletis, Fani Chatzopoulou, Dimitrios Chatzidimitriou, Fani Tsingerlioti, Christina Botziori, Georgios Tzimagiorgis, Lemonia Skoura
The emergence and spread of NDM-1-encoding Klebsiella pneumoniae is causing worldwide concern, whereas a second epicenter of their dissemination after the Indian subcontinent is thought to be located in the Balkans. In this study, the complete genome sequencing of an NDM-1-producing ST11 K. pneumoniae isolated in a private laboratory in Greece is presented. The genome sequencing was performed on Illumina MiniSeq. Multilocus Sequence Typing was determined using a BLAST-based approach whereas antimicrobial resistance genes and plasmid replicons were identified by ResFinder and PlasmidFinder respectively...
April 26, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29696375/genomic-characterization-of-nitrospirillum-amazonense-strain-cbamc-a-nitrogen-fixing-bacterium-isolated-from-surface-sterilized-sugarcane-stems
#14
Stefan Schwab, Leonardo Araujo Terra, José Ivo Baldani
Nitrospirillum amazonense is a nitrogen-fixing bacterium that shows potential to promote plant growth when inoculated into sugarcane and rice plants. This microorganism has been the subject of biochemical and genetic characterization to elucidate important functions related to host plant interaction and growth promotion, including the determination of draft genome sequences of two strains, Y2 and CBAmC, the second of which is the aim of the present study. CBAmC has been isolated from sugarcane (Saccharum spp...
April 25, 2018: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29695434/regulation-of-apolipoprotein-e-trafficking-by-hepatitis-c-virus-induced-autophagy
#15
Ja Yeon Kim, Jing-Hsiung James Ou
Apolipoprotein E (ApoE) plays an important role in the maturation and infectivity of hepatitis C virus (HCV). By analyzing the subcellular localization of ApoE in Huh7 hepatoma cells that harbored an HCV subgenomic RNA replicon, we found that ApoE colocalized with autophagosomes. This colocalization was marginally detected in HCV-infected cells apparently due to the depletion of ApoE by HCV, as the treatment with bafilomycin A1 (BafA1), a vacuolar ATPase inhibitor that inhibits autophagic protein degradation, partially restored the ApoE level and enhanced its colocalization with autophagosomes in HCV-infected cells...
April 25, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29691107/-the-controversial-burkholderia-cepacia-complex-a-group-of-plant-growth-promoting-species-and-plant-animals-and-human-pathogens
#16
Fernando U Rojas-Rojas, David López-Sánchez, Georgina Meza-Radilla, Ausel Méndez-Canarios, J Antonio Ibarra, Paulina Estrada-de Los Santos
The Burkholderia cepacia complex is a group of 22 species, which are known as opportunistic pathogens in immunocompromised people, especially those suffering from cystic fibrosis. It is also found in nosocomial infections and is difficult to eradicate due to intrinsic resistance to several antibiotics. The species have large genomes (up to 9 Mbp), distributed into 2-5 replicons. These features significantly contribute to genome plasticity, which makes them thrive in different environments like soil, water, plants or even producing nodules in legume plants...
April 21, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29686138/ddri-a-crp-family-member-acts-as-a-major-regulator-for-adaptation-of-deinococcus-radiodurans-to-various-stresses
#17
Laura Meyer, Geneviève Coste, Suzanne Sommer, Jacques Oberto, Fabrice Confalonieri, Pascale Servant, Cécile Pasternak
The <u>D</u>NA <u>d</u>amage <u>r</u>esponse gene ddrI encodes a transcription regulator belonging to the CRP (cAMP Receptor Protein) family. Cells devoid of the DdrI protein exhibit a pleiotropic phenotype, including growth defects, sensitivity to DNA damaging agents and to oxidative stress. Here, we show that the absence of DdrI protein also confers sensitivity to heat shock treatment and several genes involved in heat shock response were shown to be up-regulated in a DdrI dependent manner...
April 23, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29684127/the-presence-of-colisitn-resistance-gene-mcr-1-and-3-in-esbl-producing-escherichia-coli-isolated-from-food-in-ho-chi-minh-city-vietnam
#18
Takahiro Yamaguchi, Ryuji Kawahara, Kazuo Harada, Shihono Teruya, Tatsuya Nakayama, Daisuke Motooka, Shota Nakamura, Phuc Do Nguyen, Yuko Kumeda, Chinh Van Dang, Kazumasa Hirata, Yoshimasa Yamamoto
Colistin is indicated for the treatment of multidrug-resistant gram-negative bacterial infections. However, the spread of colistin-resistant bacteria harboring a mcr gene has become a serious concern. This study investigated local foods in Vietnam for contamination with colistin-resistant bacteria. A total of 261 extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from 330 meat and seafood products were analyzed for colistin susceptibility and the presence of mcr genes. Approximately 24% (62/261) of ESBL- or AmpC-producing E...
April 19, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29681484/design-synthesis-and-structure-activity-relationships-of-novel-imidazo-4-5-c-pyridine-derivatives-as-potent-non-nucleoside-inhibitors-of-hepatitis-c-virus-ns5b
#19
Moyi Liu, Qiaoling Xu, Su Guo, Ruixi Zuo, Yue Hong, Yong Luo, Yingxiu Li, Ping Gong, Yajing Liu
The hepatitis C virus (HCV) NS5B polymerase is an attractive target for the development of novel and selective inhibitors of HCV replication. In this paper, the design, synthesis, and preliminary SAR studies of novel inhibitors of HCV NS5B polymerase based on the structure of tegobuvir have been described. The efforts to optimize the antiviral potency and reduce the treatment side effects with respect to genotype 1b resulted in the discovery of compound 3, which exhibited an EC50 of 1.163 nM and a CC50 >200 nM in a cell-based HCV replicon system assay...
April 16, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29679645/sequencing-of-pt5282-ctxm-p13190-kpc-and-p30860-nr-and-comparative-genomics-analysis-of-incx8-plasmids
#20
Haihong Fang, Jiao Feng, Yuanhong Xu, Ying Zhang, Zhe Zhan, Zhe Yin, Xiongbo Duan, Bo Gao, Yigang Tong, Jinglin Wang, Yanping Luo, Erhei Dai, Dongsheng Zhou
This study proposed a replicon-based scheme for typing IncX into nine separately clustering subgroups, including IncX1α, IncX1β and IncX2-8. The complete nucleotide sequences of three IncX8 plasmids, namely pT5282-CTXM and p30860-NR from Enterobacter cloacae and p13190-KPC from Klebsiella pneumoniae, were determined and compared with all the other two previously sequenced IncX8 plasmids pCAV1043-58 and pCAV1741-16. These five plasmids possessed conserved IncX8 backbones with limited genetic variations with respect to gene content and organization, and each of them carried one or three accessory modules that harbored resistance markers and metabolic gene clusters as well as transposons, insertion sequence (IS)-based transposition units and miniature inverted repeat transposable elements, indicating that the relatively small IncX8 backbones were able to integrate various foreign genetic contents...
April 18, 2018: International Journal of Antimicrobial Agents
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