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Immunoturbidimetry

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https://www.readbyqxmd.com/read/28134964/association-of-plasma-complement-c3-levels-with-primary-angle-closure-glaucoma-in-older-women
#1
Shengjie Li, Yuhong Chen, Mingxi Shao, Li Tang, Xinghuai Sun, Wenjun Cao
Purpose: The plasma complement component (C)3 concentration and clinical characteristics of primary angle-closure glaucoma (PACG) subjects were analyzed to evaluate whether C3 levels were correlated with PACG severity. Methods: Peripheral blood samples from subjects with PACG (n = 237) and normal controls (n = 158) were collected. The plasma levels of C3 were measured by immunoturbidimetry. The assessments of intraocular pressure; vertical cup/disc ratio (VCDR); central corneal thickness; axial length; anterior chamber depth; visual field-derived mean deviation; and mean sensitivity were performed by glaucoma specialists...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28038785/reduced-thrombin-activatable-fibrinolysis-inhibitor-and-enhanced-proinflammatory-cytokines-in-acute-coronary-syndrome
#2
H Pang, C Zhang, F Liu, X Gong, X Jin, C Su
OBJECTIVE: A study was made of the changes in the serum levels of thrombin activatable fibrinolysis inhibitor (TAFI), proinflammatory cytokines and acute phase proteins in the acute stage of acute coronary syndrome (ACS), in order to explore the possibility of using TAFI as a biomarker for ACS risk assessment. METHODS: A total of 211 patients with ACS were enrolled, and healthy subjects were used as controls. Blood samples were taken within 24h after admission. Serum TAFI levels were determined by immunoturbidimetry...
December 27, 2016: Medicina Intensiva
https://www.readbyqxmd.com/read/27982282/effects-of-perinatal-exposure-to-nonylphenol-on-delivery-outcomes-of-pregnant-rats-and-inflammatory-hepatic-injury-in-newborn-rats
#3
J Yu, Y Luo, X F Yang, M X Yang, J Yang, X S Yang, J Zhou, F Gao, L T He, J Xu
The current study aimed to investigate the effects of perinatal exposure to nonylphenol (NP) on delivery outcome of pregnant rats and subsequent inflammatory hepatic injury in newborn rats. The pregnant rats were divided into 2 groups: control group (corn oil) and NP exposure group. Thirty-four pregnant rats were administered NP or corn oil by gavage from the sixth day of pregnancy to 21 days postpartum, with blood samples collected at 12 and 21 days of pregnancy and 60 days after delivery. The NP concentration was measured by HPLC, with chemiluminescence used for detection of estrogen and progesterone levels...
December 8, 2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/27868236/national-continuous-surveys-on-internal-quality-control-for-hba1c-in-306-clinical-laboratories-of-china-from-2012-to-2016-continual-improvement
#4
Tingting Li, Wei Wang, Haijian Zhao, Falin He, Kun Zhong, Shuai Yuan, Zhiguo Wang
BACKGROUND: This study aimed to evaluate whether the quality performance of clinical laboratories in China has been greatly improved and whether Internal Quality Control (IQC) practice of HbA1c has also been changed since National Center for Clinical Laboratories (NCCL) of China organized laboratories to report IQC data for HbA1c in 2012. METHODS: Internal Quality Control information of 306 External Quality Assessment (EQA) participant laboratories which kept reporting IQC data in February from 2012 to 2016 were collected by Web-based EQA system...
November 21, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27862036/association-of-%C3%AE-1-adrenergic-m2-muscarinic-receptor-autoantibody-with-occurrence-and-development-of-nonvalvular-atrial-fibrillation
#5
Bing Hu, Yanxiang Sun, Sha Li, Jie Sun, Tong Liu, Zidi Wu, L I Feng
BACKGROUND: Atrial fibrillation (AF) is the most common sustained arrhythmia and is independently associated with increased risk of stroke and death. Although the exact mechanisms of AF are not completely elucidated, a large number of evidences demonstrate that autoimmunity may play an important role in the initiation, the progression, and the maintenance of AF. In this study, we aimed to compare anti-β1-adrenergic receptor autoantibody (anti-β1-R) and anti-M2-muscarinic receptor autoantibody (anti-M2-R) levels between nonvalvular AF patients and healthy control subjects...
December 2016: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/27831647/the-clinical-significance-of-level-changes-of-hs-crp-il-10-and-tnf-for-patients-with-ms-during-active-and-relieving-period
#6
A-L Ji, Z-H Liu, W-W Chen, W-J Huang
OBJECTIVE: To investigate the clinical significance of the change of the level of hs-CRP, interleukin-10 (IL-10) and tumor necrosis factors (TNF-α) for patients with multiple sclerosis (MS) during the active and the relieving period. PATIENTS AND METHODS: 36 patients with MS during the active stage and the relieving stage after therapy (experimental group) were compared with 10 healthy people at the relieving period, considered as control group. Immunoturbidimetry was used to examine levels of hs-CRP while the level of IL-10 and TNF-a were tested by enzyme-linked immunosorbent assay (ELISA)...
October 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27770201/histopathological-and-immunological-changes-during-the-acute-and-recovery-phase-in-henoch-sch%C3%A3-nlein-purpura-rabbit-model
#7
Yanhong Li, Xiaolong Sui, Hua Zhu, Yanfeng Xu, Lan Huang, Yuhuan Xu, Yunlin Han, Xiaochun Feng, Chuan Qin
Henoch-Schönlein purpura (HSP) is a systemic vasculitis mediated by autologous immune complex. Animal models of HSP are scarce. Here, we describe the characteristics of HSP rabbit model in the acute and recovery phase. First, we constructed the HSP rabbit models, and then assessed immunologic indicators of models by enzyme-linked immunosorbent assay and immunoturbidimetry. Histomorphological characteristics were analyzed by haematoxylin-eosin, immunofluorescence and special staining. In the acute stage (24 h) after antigen challenge, the model group rabbits featured skin ecchymosis and abnormal laboratory examination results...
January 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/27743141/double-positivity-of-the-igg-isotype-of-both-anticardiolipin-and-anti-%C3%AE-2gpi-antibodies-is-associated-with-the-highest-number-of-vascular-impairment-parameters-in-patients-with-primary-antiphospholipid-syndrome-preliminary-data
#8
Mirjana Bećarević, Duško Mirković, Svetlana Ignjatović
Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-α), C-reactive protein (CRP) and apolipoproteins (apos) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS...
December 2016: Clinical Rheumatology
https://www.readbyqxmd.com/read/27737645/haemodiafiltration-elicits-less-platelet-activation-compared-to-haemodialysis
#9
Gergely Becs, Renáta Hudák, Zsolt Fejes, Ildikó Beke Debreceni, Harjit Pal Bhattoa, József Balla, János Kappelmayer
BACKGROUND: Mortality in patients with end-stage renal disorders is often a consequence of cardiovascular complications. Renal replacement therapies may contribute to this morbidity by promoting cellular activation. In renal failure patients peripheral blood samples were investigated for platelet and endothelial cell activation markers to compare the effects of haemodiafiltration (HDF) and haemodialysis (HD). METHODS: Overall 28 patients were included in the study...
October 13, 2016: BMC Nephrology
https://www.readbyqxmd.com/read/27684817/three-cases-of-congenital-dysfibrinogenemia-in-unrelated-chinese-families-heterozygous-missense-mutation-in-fibrinogen-alpha-chain-argl6his
#10
Meiling Luo, Donghong Deng, Liqun Xiang, Peng Cheng, Lin Liao, Xuelian Deng, Jie Yan, Faquan Lin
Congenital dysfibrinogenemia (CD) is a qualitative fibrinogen disorder caused by an abnormal fibrinogen molecule structure, leading to dysfunctional blood coagulation. This study describes 3 cases of dysfibrinogenemia identified in the unrelated Chinese pedigrees.Routine coagulation screening tests were performed on the probands and their families. The antigens and functionality of fibrinogen was measured using an immunoturbidimetry assay and the Clauss method, respectively. To identify the genetic mutation responsible for these dysfibrinogens, genomic DNA extracted from the blood was analyzed using PCR amplification and direct sequencing...
September 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27669631/th1-th17-cxcl16-and-homocysteine-elevated-after-intracranial-and-cervical-stent-implantation
#11
Yanyan Tang, Yunfei Wei, Ziming Ye, Chao Qin
The presence of Th1 and Th17 cells have been observed as major inducers in inflammation and immune responses associated stenting. However, there is rare data on the impact of Th1, Th17, CXCL16 and homocysteine after cerebral stent implantation. Here, we performed the statistical analysis to firstly evaluate the variation of the Th17, Th1 cells and their related cytokines, CXCL16 and homocysteine in the peripheral blood of patients with cerebral stenting. The flow cytometry were used to detect the proportion of Th1 and Th17 cells in peripheral blood mononuclear cells...
September 27, 2016: International Journal of Neuroscience
https://www.readbyqxmd.com/read/27666762/a-global-multicenter-study-on-reference-values-2-exploration-of-sources-of-variation-across-the-countries
#12
Kiyoshi Ichihara, Yesim Ozarda, Julian H Barth, George Klee, Yoshihisa Shimizu, Liangyu Xia, Mariza Hoffmann, Swarup Shah, Tandi Matsha, Janette Wassung, Francois Smit, Anna Ruzhanskaya, Joely Straseski, Daniel N Bustos, Shogo Kimura, Aki Takahashi
OBJECTIVES: The intent of this study, based on a global multicenter study of reference values (RVs) for serum analytes was to explore biological sources of variation (SVs) of the RVs among 12 countries around the world. METHODS: As described in the first part of this paper, RVs of 50 major serum analytes from 13,396 healthy individuals living in 12 countries were obtained. Analyzed in this study were 23 clinical chemistry analytes and 8 analytes measured by immunoturbidimetry...
September 22, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27605239/soluble-transferrin-receptor-levels-are-positively-associated-with-insulin-resistance-but-not-with-the-metabolic-syndrome-or-its-individual-components
#13
Milton Fabian Suárez-Ortegón, Stela McLachlan, Sarah H Wild, José Manuel Fernández-Real, Caroline Hayward, Ozren Polašek
The metabolic syndrome (MetS) is known to be associated with elevated serum ferritin levels. The possible association with other Fe markers has been less well studied. We aimed to investigate the cross-sectional association of soluble transferrin receptor (sTfR) and ferritin levels with the MetS components, insulin resistance and glycosylated Hb (HbA1C). The sample consisted of 725 adults, aged 19-93 years (284 men, 151 premenopausal and 290 postmenopausal women), from the Croatian island of Vis. Serum sTfR and ferritin levels were measured by immunoturbidimetry and electrochemiluminescence assays, respectively...
October 2016: British Journal of Nutrition
https://www.readbyqxmd.com/read/27534721/influence-of-elevated-crp-level-related-polymorphisms-in-non-rheumatic-caucasians-on-the-risk-of-subclinical-atherosclerosis-and-cardiovascular-disease-in-rheumatoid-arthritis
#14
Raquel López-Mejías, Fernanda Genre, Sara Remuzgo-Martínez, Carlos González-Juanatey, Montserrat Robustillo-Villarino, Javier Llorca, Alfonso Corrales, Esther Vicente, José A Miranda-Filloy, César Magro, Beatriz Tejera-Segura, Marco A Ramírez Huaranga, Trinitario Pina, Ricardo Blanco, Juan J Alegre-Sancho, Enrique Raya, Verónica Mijares, Begoña Ubilla, María D Mínguez Sánchez, Carmen Gómez-Vaquero, Alejandro Balsa, Dora Pascual-Salcedo, Francisco J López-Longo, Patricia Carreira, Isidoro González-Álvaro, Luis Rodríguez-Rodríguez, Benjamín Fernández-Gutiérrez, Iván Ferraz-Amaro, Santos Castañeda, Javier Martín, Miguel A González-Gay
Association between elevated C-reactive protein (CRP) serum levels and subclinical atherosclerosis and cardiovascular (CV) events was described in rheumatoid arthritis (RA). CRP, HNF1A, LEPR, GCKR, NLRP3, IL1F10, PPP1R3B, ASCL1, HNF4A and SALL1 exert an influence on elevated CRP serum levels in non-rheumatic Caucasians. Consequently, we evaluated the potential role of these genes in the development of CV events and subclinical atherosclerosis in RA patients. Three tag CRP polymorphisms and HNF1A, LEPR, GCKR, NLRP3, IL1F10, PPP1R3B, ASCL1, HNF4A and SALL1 were genotyped in 2,313 Spanish patients by TaqMan...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27522723/-insulin-resistance-associated-with-metabolic-syndrome-as-an-indicator-of-cardiovascular-risk
#15
A V Dontsov, L V Vasil'eva
AIM: To study blood insulin level in patients with coronary heart disease (CHD) with and without metabolic syndrome (MS) and its relation to cardiovascular risk factors. MATERIALS AND METHODS: We examined 127 patients with stable coronary heart disease (mean age 59.4 ± 5.7 yr) including 63 with MS and 64 without it. The control group consisted of 80 practically healthy subjects. Bloods insulin was determined by immunochemoluminescence, glycated hemoglobin (HbA,) by immunoturbidimetry, total cholesterol, HDLP cholesterol and triglycerides by enzymatic colorimetric method, oxidized LDLP IL-l, IL-6, and tunour necrosis factor-α by enzyme immunoassay...
2016: Klinicheskaia Meditsina
https://www.readbyqxmd.com/read/27338061/predictive-value-of-plasma-%C3%AE-2-microglobulin-on-human-body-function-and-senescence
#16
X-M Dong, R Cai, F Yang, Y-Y Zhang, X-G Wang, S-L Fu, J-R Zhang
OBJECTIVE: To explore the correlation between plasma β2-microglobulin (β2-MG) as senescence factor with age, heart, liver and kidney function as well as the predictive value of β2-MG in human metabolism function and senescence. PATIENTS AND METHODS: 387 cases of healthy people of different ages were selected and the automatic biochemical analyzer was used to test β2-MG in plasma based on immunoturbidimetry and also all biochemical indexes. The correlation between β2-MG and age, gender and all biochemical indexes was analyzed...
June 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27248178/high-serum-haptoglobin-level-is-associated-with-tumor-progression-and-predicts-poor-prognosis-in-non-small-cell-lung-cancer
#17
Jianjun Lu, Yanhong Wang, Miansheng Yan, Pinning Feng, Linjing Yuan, Yuesu Cai, Xin Xia, Min Liu, Jinmei Luo, Laisheng Li
The overall survival time of non-small cell lung cancer (NSCLC) has not improved dramatically in recent decades. An important reason is the lacking of valuable biomarkers. Haptoglobin was reported to have activities of anti-inflammatory, anti-oxidant, autoimmune and tumor angiogenesis. However its potential role as a tumor biomarker was not well recognized. We used an immunoturbidimetry method to measure serum haptoglobin levels in 205 NSCLC patients, and 210 normal healthy controls. We found that serum haptoglobin levels were significantly elevated in NSCLC patients compared with normal healthy controls (1...
July 5, 2016: Oncotarget
https://www.readbyqxmd.com/read/27242125/associations-of-serum-anti-ganglioside-antibodies-and-inflammatory-markers-in-diabetic-peripheral-neuropathy
#18
Shengjie Ge, Jing Xie, Lequn Zheng, Lijuan Yang, Hong Zhu, Xingbo Cheng, Feixia Shen
AIMS: To investigate the associations between inflammatory markers, serum anti-ganglioside antibodies (anti-GS-ab), serum plasminogen activator inhibitor-1 (PAI-1), tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), and diabetic peripheral neuropathy (DPN). METHODS: Study subjects were divided into three groups: normal group (N group) with 101 healthy individuals; diabetes mellitus without peripheral neuropathy group (DM group) with 87 patients; and DPN group with 178 cases...
May 2016: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/27241883/determination-of-urine-albumin-by-new-simple-high-performance-liquid-chromatography-method
#19
Eva Klapkova, Magdalena Fortova, Richard Prusa, Libuse Moravcova, Karel Kotaska
BACKGROUND: A simple high-performance liquid chromatography (HPLC) method was developed for the determination of albumin in patients' urine samples without coeluting proteins and was compared with the immunoturbidimetric determination of albumin. Urine albumin is important biomarker in diabetic patients, but part of it is immuno-nonreactive. METHODS: Albumin was determined by high-performance liquid chromatography (HPLC), UV detection at 280 nm, Zorbax 300SB-C3 column...
November 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27235671/elevated-serum-adiponectin-is-related-to-elevated-serum-ferritin-and-interleukin-6-in-%C3%AE-thalassaemia-major-children
#20
Farida H El-Rasheidy, Enas S Essa, Asmaa A S Mahmoud, Abd El-Wahab A A Nada
BACKGROUND: There is evidence that iron affects lipid metabolism and adipocyte biology. Given the effects of iron on adiponectin, the role of iron in lipid oxidation, and the potential additive effects of oxidative stress from excess iron and lipid metabolism. We aimed to investigate serum adiponectin in relation to clinical and laboratory parameters including the inflammation markers [C-reacitve protein (CRP) and interleukin-6 (IL-6)] in β-thalassaemia major children. METHODS: We investigated 58 β-thalassaemia major children under scheduled blood transfusion and 30 controls...
August 1, 2016: Journal of Pediatric Endocrinology & Metabolism: JPEM
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