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Immunoturbidimetry

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https://www.readbyqxmd.com/read/29068574/total-antioxidant-capacity-in-mediterranean-%C3%AE-thalassemic-patients
#1
Ioannis Tsamesidis, Claudio Fozza, Eleni Vagdatli, Anastasia Kalpaka, Carla Cirotto, Maria Carmina Pau, Antonella Panataleo, Francesco Turrini, Elisavet Grigoriou, Eugenia Lymperaki
BACKGROUND: Beta thalassemia major (BT) is an inherited blood disorder caused by reduced or absent synthesis of the hemoglobin beta chains, associated with profound anemia, jaundice, splenomegaly, expanded bone marrow volume, siderosis and cardiomegaly. Because of repeated blood transfusions, BT patients are subjected to peroxidative tissue injury due to secondary iron overload. OBJECTIVES: The aim of the study was to analyze: 1) the total antioxidant capacity (TAC) value in BT patients (study group) and their healthy controls (control group) from Greece (Central Macedonia) and Italy (Sardinia); correlations between 2) the TAC and ferritin levels of BT patients, and 3) the TAC and ferritin values in BT patients with different chelation therapies...
August 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28973339/association-between-serum-complement-c3-levels-and-age-related-cataract
#2
Mingxi Shao, Danhui Li, Jisen Teng, Yudong Zhang, Shengjie Li, Wenjun Cao
Purpose: The serum complement component 3 (C3) concentration and clinical characteristics of age-related cataract (ARC) subjects were analyzed to evaluate whether serum C3 levels are correlated with ARC. Methods: A total of 492 ARC patients and 466 normal subjects from the Department of Ophthalmology and Visual Science, Eye and ENT Hospital of Fudan University, Shanghai, China, were consecutively recruited into this study between June 2014 and May 2017. Immunoturbidimetry was used to measure the serum C3 levels...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28901563/analysis-of-serum-levels-and-cutaneous-expression-of-lipoprotein-a-in-38-patients-with-livedoid-vasculopathy
#3
Danielle P G S Espinel, Thais B Di Giacomo, Thais P Pincelli, Naiura V Pereira, Miriam N Sotto, Robert S Kirsner, Paulo R Criado
BACKGROUND: Coagulation disorders contribute to the development of livedoid vasculopathy (LV). Elevated plasma levels of lipoprotein(a) [Lp(a)] are an independent risk factor for the development of cardiovascular disease and associated with hypercoagulable states. Increased serum Lp(a) levels have been reported in patients with LV and may have an important role in the pathogenesis of LV. OBJECTIVES: To investigate Lp(a) expression in skin lesions and circulating serum Lp(a) levels in patients with LV...
September 13, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28894708/hepatic-proteins-and-inflammatory-markers-in-rheumatoid-arthritis-patients
#4
Manel Ben-Hadj-Mohamed, Souhir Khelil, Mokhles Ben Dbibis, Latifa Khlifi, Hend Chahed, Salima Ferchichi, Elyes Bouajina, Abdelhédi Miled
BACKGROUND: Rheumatoid arthritis is an autoimmune inflammatory rheumatic disease that causes chronic synovial inflammation eventually leading to joint destruction and disability. The aim of this study was to determine the variations of hepatic proteins, myeloperoxidase, and iron in rheumatoid arthritis Tunisian patients and their implications in inflammation and in iron metabolism. METHODS: Overall, 172 patients from the Rheumatology Department of the University Hospital "Farhat Hached", Sousse-Tunisia between 2011 and 2012, with rheumatoid arthritis (97...
August 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28829488/the-effect-of-dexmedetomidine-on-expressions-of-inflammatory-factors-in-patients-with-radical-resection-of-gastric-cancer
#5
W Dong, M-H Chen, Y-H Yang, X Zhang, M-J Huang, X-J Yang, H-Z Wang
OBJECTIVE: To investigate the effect of dexmedetomidine on the expressions of inflammatory factors, T-lymphocyte subgroups and nuclear factor kappa-B (NF-κB) in peripheral blood monocytes in the perioperative period of radical resection of gastric cancer. PATIENTS AND METHODS: We selected 74 patients who were admitted to our hospital for radical resection of gastric cancer between January 2012 and October 2015. All patients were randomly divided into the dexmedetomidine group and the control group...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28672914/the-levels-of-serum-pro-calcitonin-and-high-sensitivity-c-reactive-protein-in-the-early-diagnosis-of-chronic-obstructive-pulmonary-disease-during-acute-exacerbation
#6
Dejie Gao, Xincan Chen, He Wu, Haidong Wei, Jinling Wu
The level of of procalcitonin (PCT) and high-sensitivity C-reactive protein (hs-CRP) in the acute exacerbation of COPD (AECOPD) was investigated. Total of 20 patients with acute exacerbation of COPD who were admitted to the Department of Respiratory Medicine, Binzhou Center Hospital in the period of October 2012 to April 2015 were enrolled in the AECOPD group. According to the color of the sputum, the patients with AECOPD were divided into purulent sputum group (n=8) and non-purulent sputum group (n=12). In addition, 15 healthy people from the outpatient medical center were also selected as healthy control group...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28661913/soluble-endothelial-cell-molecules-and-circulating-endothelial-cells-in-patients-with-venous-thromboembolism
#7
Cláudia Torres, Rui Matos, Sara Morais, Manuel Campos, Margarida Lima
: To evaluate the plasma levels of soluble endothelial cell molecules in patients with venous thromboembolism (VTE) out of the acute phase as compared with healthy individuals. We also investigated the possible associations of the soluble endothelial cell molecules among them, as well as with other clinical and laboratory data, including the numbers of circulating endothelial cells (CEC), circulating endothelial progenitor cells (CEP), and CEC expressing activation-related [cluster of differentiation (CD)54 and CD62E] and procoagulant (CD142) markers...
June 28, 2017: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/28636270/c-reactive-protein-and-progression-of-vision-loss-in-retinitis-pigmentosa
#8
Yusuke Murakami, Yasuhiro Ikeda, Shunji Nakatake, Kohta Fujiwara, Takashi Tachibana, Noriko Yoshida, Shoji Notomi, Toshio Hisatomi, Shigeo Yoshida, Tatsuro Ishibashi, Koh-Hei Sonoda
PURPOSE: Chronic inflammation is involved in retinitis pigmentosa (RP). We demonstrated previously that intraocular inflammatory levels, as measured by slit-lamp ophthalmoscopy or laser flare photometry, are inversely correlated with central visual function in patients with RP. Here, we investigated the relationship between serum high-sensitivity C-reactive protein (hs-CRP) and visual parameters in RP. METHODS: We studied 58 consecutive typical patients with RP <40 years old and 29 age- and gender-matched controls...
June 21, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28611236/quantitation-of-circulating-gdf-11-and-%C3%AE-2-mg-in-aged-patients-with-age-related-impairment-in-cognitive-function
#9
Rungong Yang, Shuhong Fu, Liang Zhao, Bei Zhen, Ling Ye, Xiaolu Niu, Xiaoxia Li, Pumin Zhang, Jie Bai
Growth differentiation factor 11 (GDF-11) has been implicated in reverse effects of ageing on the central nervous system of humans. β2-microglobulin (β2-MG) has been reported to negatively regulate cognition. However, there is a lot of controversy about the role of GDF-11 and β2-MG in ageing and cognitive regulation. To examine the involvement of GDF-11 and β2-MG in the ageing process and cognitive dysfunction, a total of 51 healthy subjects and 41 elderly patients with different degrees of age-related cognitive impairment participated in the study...
August 1, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28571126/study-of-glycated-haemoglobin-hba1c-in-non-diabetic-subjects-with-subclinical-hypothyroidism
#10
Mayur Goradhanbhai Makadia, Vishwal Indravadan Patel, Kinjal Prahaladbhai Patel, Aashna Darshanbhai Shah, Kaushik Salubhai Chaudhari, Hitesh Natvarbhai Shah, Haridas Neelakandan Nilayangode
INTRODUCTION: India has the higher prevalence of prediabetes, diabetes and hypothyroidism. Subclinical Hypothyroidism (SH) is more prevalent in the females than in the males. Studies have shown the elevated HbA1c in the non-diabetic hypothyroid patients. SH is defined by normal serum triiodothyronine (T3), thyroxine (T4) levels and serum Thyroid Stimulating Hormone (TSH) level falling between 4.2 to 10 mU/L. AIM: To study the HbA1c level in the non-diabetic SH patients and to compare the HbA1c level with the controls...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28540853/determination-of-serum-albumin-analytical-challenges-a-french-multicenter-study
#11
Adrien Rossary, Françoise Blondé-Cynober, Jean-Philippe Bastard, Marie-Christine Beauvieux, Pascale Beyne, Jocelyne Drai, Christine Lombard, Ingrid Anglard, Christian Aussel, Sophie Claeyssens, Marie-Paule Vasson
Among the biological markers of morbidity and mortality, albumin holds a key place in the range of criteria used by the High Authority for Health (HAS) for the assessment of malnutrition and the coding of information system medicalization program (PMSI). If the principle of quantification methods have not changed in recent years, the dispersion of external evaluations of the quality (EEQ) data shows that the standardization using the certified reference material (CRM) 470 is not optimal. The aim of this multicenter study involving 7 sites, conducted by a working group of the French Society of Clinical Biology (SFBC), was to assess whether the albuminemia values depend on the analytical system used...
June 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28464873/highly-sensitive-and-accurate-detection-of-c-reactive-protein-by-cdse-zns-quantum-dot-based-fluorescence-linked-immunosorbent-assay
#12
Yanbing Lv, Ruili Wu, Kunrui Feng, Jinjie Li, Qing Mao, Hang Yuan, Huaibin Shen, Xiangdong Chai, Lin Song Li
BACKGROUND: The conventional and widely used enzyme-linked immunosorbent assays (ELISA), due to specificity and high-sensitivity, were suitable in vitro diagnosis. But enzymes are vulnerable to the external conditions, and the complex operation steps limit its application. Semiconductor quantum dots have been successfully used in biological and medical research due to the high photoluminescence and high resistance to photobleaching. In this study, we have developed a novel quantum dot-labeled immunosorbent assay for rapid disease detection of C-reactive protein (CRP)...
May 2, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28461157/cholesterol-delivery-to-the-adrenal-glands-estimated-by-adrenal-venous-sampling-an-in%C3%A2-vivo-model-to-determine-the-contribution-of-circulating-lipoproteins-to-steroidogenesis-in-humans
#13
Edward Buitenwerf, Robin P F Dullaart, Anneke C Muller Kobold, Thera P Links, Wim J Sluiter, Margery A Connelly, Michiel N Kerstens
BACKGROUND: Cholesterol, required for adrenal steroid hormone synthesis, is at least in part derived from circulating lipoproteins. The contribution of high-density lipoproteins (HDL) and low-density lipoproteins (LDL) to adrenal steroidogenesis in humans is unclear. OBJECTIVE: The aim of the study was to determine the extent to which HDL and LDL are taken up by the adrenal glands using samples obtained during adrenal venous sampling (AVS). METHODS: AVS was successfully performed in 23 patients with primary aldosteronism...
May 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/28409899/brief-report-genetic-variation-of-the-%C3%AE-1-antitrypsin-gene-is-associated-with-increased-autoantibody-production-in-rheumatoid-arthritis
#14
Cormac McCarthy, Carl Orr, Laura T Fee, Tomás P Carroll, Danielle M Dunlea, David J L Hunt, Eimear Dunne, Paul O'Connell, Geraldine McCarthy, Dermot Kenny, Ursula Fearon, Douglas J Veale, Emer P Reeves, Noel G McElvaney
OBJECTIVE: To examine the prevalence of α1 -antitrypsin deficiency (AATD) in rheumatoid arthritis (RA), and to determine whether AATD is associated with higher levels of rheumatoid factor (RF), antinuclear antibodies (ANAs), and anti-citrullinated peptide autoantibodies (ACPAs). METHODS: RF, ANAs, and ACPAs were measured by standard immunoturbidimetry, immunofluorescence assay, and enzyme-linked immunosorbent assay, respectively. Characterization of AAT phenotypes was performed by isoelectric focusing and immunofixation...
August 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28392828/screening-of-wilson-s-disease-in-a-psychiatric-population-difficulties-and-pitfalls-a-preliminary-study
#15
Caroline Demily, François Parant, David Cheillan, Emmanuel Broussolle, Alice Pavec, Olivier Guillaud, Lioara Restier, Alain Lachaux, Muriel Bost
BACKGROUND: Wilson's disease (WD) is a rare autosomal-recessive, inherited disorder caused by a mutation in the copper-transporting gene ATP7B affecting the liver and nervous system. About 30% of patients with WD may initially present with psychiatric symptoms, and diagnosis can be difficult to establish. The objectives of the present preliminary study were [1] to evaluate the relevance of serum copper (Cu) and ceruloplasmin (Cp) measures in hospitalized patients with psychiatric disorders; and [2] to identify possible mutations in the ATP7B gene in patients with abnormal biological copper profile...
2017: Annals of General Psychiatry
https://www.readbyqxmd.com/read/28386638/effect-of-diet-induced-weight-loss-on-lipoprotein-a-levels-in-obese-individuals-with-and-without-type-2-diabetes
#16
Kirsten A Berk, Reyhana Yahya, Adrie J M Verhoeven, Jeanette Touw, Frank P Leijten, Elisabeth F van Rossum, Vincent L Wester, Mirjam A Lips, Hanno Pijl, Reinier Timman, Gertraud Erhart, Florian Kronenberg, Jeanine E Roeters van Lennep, Eric J G Sijbrands, Monique T Mulder
AIMS/HYPOTHESIS: Elevated levels of lipoprotein(a) [Lp(a)] are an independent risk factor for cardiovascular disease (CVD), particularly in individuals with type 2 diabetes. Although weight loss improves conventional risk factors for CVD in type 2 diabetes, the effects on Lp(a) are unknown and may influence the long-term outcome of CVD after diet-induced weight loss. The aim of this clinical study was to determine the effect of diet-induced weight loss on Lp(a) levels in obese individuals with type 2 diabetes...
June 2017: Diabetologia
https://www.readbyqxmd.com/read/28377336/immunoanalytical-characteristics-of-c-reactive-protein-and-high-sensitivity-c-reactive-protein
#17
REVIEW
Mariame Moutachakkir, Asma Lamrani Hanchi, Azzedine Baraou, Abderrahman Boukhira, Saliha Chellak
C-reactive protein (CRP) is a polypeptide molecule belonging to the family of pentraxins. It has a molecular mass of 120,000 daltons and consists of five identical sub-units that contain each 206 amino acids. CRP is synthesized primarily by the liver in response to certain pro-inflammatory cytokines. It plays an important role in innate immunity, opsonization by its properties, complement activation and immunoglobulins receptor binding. CRP is a protein of the acute systemic inflammation and is, therefore, a prime marker of inflammation...
April 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28134964/association-of-plasma-complement-c3-levels-with-primary-angle-closure-glaucoma-in-older-women
#18
Shengjie Li, Yuhong Chen, Mingxi Shao, Li Tang, Xinghuai Sun, Wenjun Cao
Purpose: The plasma complement component (C)3 concentration and clinical characteristics of primary angle-closure glaucoma (PACG) subjects were analyzed to evaluate whether C3 levels were correlated with PACG severity. Methods: Peripheral blood samples from subjects with PACG (n = 237) and normal controls (n = 158) were collected. The plasma levels of C3 were measured by immunoturbidimetry. The assessments of intraocular pressure; vertical cup/disc ratio (VCDR); central corneal thickness; axial length; anterior chamber depth; visual field-derived mean deviation; and mean sensitivity were performed by glaucoma specialists...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28038785/reduced-thrombin-activatable-fibrinolysis-inhibitor-and-enhanced-proinflammatory-cytokines-in-acute-coronary-syndrome
#19
H Pang, C Zhang, F Liu, X Gong, X Jin, C Su
OBJECTIVE: A study was made of the changes in the serum levels of thrombin activatable fibrinolysis inhibitor (TAFI), proinflammatory cytokines and acute phase proteins in the acute stage of acute coronary syndrome (ACS), in order to explore the possibility of using TAFI as a biomarker for ACS risk assessment. METHODS: A total of 211 patients with ACS were enrolled, and healthy subjects were used as controls. Blood samples were taken within 24h after admission. Serum TAFI levels were determined by immunoturbidimetry...
December 27, 2016: Medicina Intensiva
https://www.readbyqxmd.com/read/27982282/effects-of-perinatal-exposure-to-nonylphenol-on-delivery-outcomes-of-pregnant-rats-and-inflammatory-hepatic-injury-in-newborn-rats
#20
J Yu, Y Luo, X F Yang, M X Yang, J Yang, X S Yang, J Zhou, F Gao, L T He, J Xu
The current study aimed to investigate the effects of perinatal exposure to nonylphenol (NP) on delivery outcome of pregnant rats and subsequent inflammatory hepatic injury in newborn rats. The pregnant rats were divided into 2 groups: control group (corn oil) and NP exposure group. Thirty-four pregnant rats were administered NP or corn oil by gavage from the sixth day of pregnancy to 21 days postpartum, with blood samples collected at 12 and 21 days of pregnancy and 60 days after delivery. The NP concentration was measured by HPLC, with chemiluminescence used for detection of estrogen and progesterone levels...
December 8, 2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
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