keyword
MENU ▼
Read by QxMD icon Read
search

Sorting nexin

keyword
https://www.readbyqxmd.com/read/29768205/c-elegans-blastomeres-clear-the-corpse-of-the-second-polar-body-by-lc3-associated-phagocytosis
#1
Gholamreza Fazeli, Maurice Stetter, Jaime N Lisack, Ann M Wehman
To understand how undifferentiated pluripotent cells cope with cell corpses, we examined the clearance of polar bodies born during female meiosis. We found that polar bodies lose membrane integrity and expose phosphatidylserine in Caenorhabditis elegans. Polar body signaling recruits engulfment receptors to the plasma membrane of embryonic blastomeres using the PI3K VPS-34, RAB-5 GTPase and the sorting nexin SNX-6. The second polar body is then phagocytosed using receptor-mediated engulfment pathways dependent on the Rac1 ortholog CED-10 but undergoes non-apoptotic programmed cell death independent of engulfment...
May 15, 2018: Cell Reports
https://www.readbyqxmd.com/read/29755290/retromer-dysfunction-and-neurodegenerative-disease
#2
REVIEW
Christiane Reitz
In recent years, genomic, animal and cell biology studies have implicated deficiencies in retromer-mediated trafficking of proteins in an increasing number of neurodegenerative diseases including Alzheimer's Disease (AD), Parkinson's Disease (PD) and Frontotemporal Lobar Degener-ation (FTLD). The retromer complex, which is highly conserved across all eukaryotes, regulates the sorting of transmembrane proteins out of endo-somes to the cell surface or to the trans-Golgi network. Within retromer, cargo selection and binding are performed by a trimer of the Vps26, Vps29 and Vps35 proteins, named the "Cargo-Selective Complex (CSC)"...
May 2018: Current Genomics
https://www.readbyqxmd.com/read/29743363/comparative-characterization-of-the-sindbis-virus-proteome-from-mammalian-and-invertebrate-hosts-identifies-nsp2-as-a-component-of-the-virus-nucleocapsid-and-sorting-nexin-5-as-a-significant-host-factor-for-alphavirus-replication
#3
Ryan Schuchman, Andy Kilianski, Amanda Piper, Ricardo Vancini, José M C Ribeiro, Thomas R Sprague, Farooq Nasar, Gabrielle Boyd, Raquel Hernandez, Trevor Glaros
Recent advances in mass spectrometry methods and instrumentation now allow for more accurate identification of proteins in low abundance. This technology was applied to Sindbis virus, the prototypical alphavirus to investigate the viral proteome. To determine if host proteins are specifically packaged into alphavirus virions, Sindbis virus (SINV) was grown in multiple host cells representing vertebrate and mosquito hosts and total protein content of purified virions was determined. This analysis identified host factors not previously associated with alphavirus entry, replication, or egress...
May 9, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29676464/the-endosomal-recycling-of-fgsnc1-by-fgsnx41-fgsnx4-heterodimer-is-essential-for-polarized-growth-and-pathogenicity-in-fusarium-graminearum
#4
Wenhui Zheng, Yahong Lin, Wenqin Fang, Xu Zhao, Yi Lou, Guanghui Wang, Huawei Zheng, Qifu Liang, Yakubu Saddeeq Abubakar, Stefan Olsson, Jie Zhou, Zonghua Wang
Endosomal sorting machineries regulate the transport of their cargoes among intracellular compartments. However, the molecular nature of such intracellular trafficking processes in pathogenic fungal development and pathogenicity remains unclear. Here, we dissect the roles and molecular mechanisms of two sorting nexin proteins and their cargoes in endosomal recycling in Fusarium graminearum using high-resolution microscopy and high-throughput co-immunoprecipitation strategies. We show that the sorting nexins, FgSnx41 and FgSnx4, interact with each other and assemble into a functionally interdependent heterodimer through their respective BAR domains...
April 20, 2018: New Phytologist
https://www.readbyqxmd.com/read/29675452/attaching-and-effacing-pathogens-exploit-junction-regulatory-activities-of-n-wasp-and-snx9-to-disrupt-the-intestinal-barrier
#5
John J Garber, Emily M Mallick, Karen M Scanlon, Jerrold R Turner, Michael S Donnenberg, John M Leong, Scott B Snapper
Background & Aims: Neural Wiskott-Aldrich Syndrome protein (N-WASP) is a key regulator of the actin cytoskeleton in epithelial tissues and is poised to mediate cytoskeletal-dependent aspects of apical junction complex (AJC) homeostasis. Attaching-and-effacing (AE) pathogens disrupt this homeostasis through translocation of the effector molecule early secreted antigenic target-6 (ESX)-1 secretion-associated protein F (EspF). Although the mechanisms underlying AJC disruption by EspF are unknown, EspF contains putative binding sites for N-WASP and the endocytic regulator sorting nexin 9 (SNX9)...
March 2018: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29668686/a-noncanonical-role-for-dynamin-1-in-regulating-early-stages-of-clathrin-mediated-endocytosis-in-non-neuronal-cells
#6
Saipraveen Srinivasan, Christoph J Burckhardt, Madhura Bhave, Zhiming Chen, Ping-Hung Chen, Xinxin Wang, Gaudenz Danuser, Sandra L Schmid
Dynamin Guanosine Triphosphate hydrolases (GTPases) are best studied for their role in the terminal membrane fission process of clathrin-mediated endocytosis (CME), but they have also been proposed to regulate earlier stages of CME. Although highly enriched in neurons, dynamin-1 (Dyn1) is, in fact, widely expressed along with Dyn2 but inactivated in non-neuronal cells via phosphorylation by glycogen synthase kinase-3 beta (GSK3β) kinase. Here, we study the differential, isoform-specific functions of Dyn1 and Dyn2 as regulators of CME...
April 18, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29667289/endosomal-receptor-trafficking-retromer-and-beyond
#7
REVIEW
Jing Wang, Alina Fedoseienko, Bayou Chen, Ezra Burstein, Da Jia, Daniel D Billadeau
The tubular endolysosomal network is a quality control system that ensures the proper delivery of internalized receptors to specific subcellular destinations in order to maintain cellular homeostasis. Although retromer was originally described in yeast as a regulator of endosome-to-Golgi receptor recycling, mammalian retromer has emerged as a central player in endosome-to-plasma membrane recycling of a variety of receptors. Over the past decade, information regarding the mechanism by which retromer facilitates receptor trafficking has emerged, as has the identification of numerous retromer-associated molecules including the WASH complex, sorting nexins and TBC1d5...
April 18, 2018: Traffic
https://www.readbyqxmd.com/read/29666193/lmo1656-is-a-secreted-virulence-factor-of-listeria-monocytogenes-that-interacts-with-the-sortin-nexin-6-bar-complex
#8
Daryl Jason David, Alessandro Pagliuso, Lilliana Radoshevich, Marie-Anne Nahori, Pascale Cossart
Listeria monocytogenes (Lm) is a facultative intracellular bacterial pathogen, the causative agent of listeriosis, a rare but fatal disease.  During infection, Lm can traverse several physiological barriers; it can cross the intestine and placenta, and in immunocompromised individuals, the blood-brain barrier. With the recent plethora of sequenced genomes available for Lm, it is clear that the complete repertoire of genes used by Lm to interact with its host remains to be fully explored.  Recently, we focused on secreted Lm proteins, since they are likely to interact with host cell components...
April 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29622675/sorting-nexin-9-snx9-regulates-levels-of-the-transmembrane-disintegrin-and-metalloproteinase-adam-9-at-the-cell-surface
#9
Kasper Johansen Mygind, Theresa Störiko, Marie L Freiberg, Jacob Samsøe-Petersen, Jeanette Schwarz, Olav M Andersen, Marie Kveiborg
ADAM9 is an active member of the family of transmembrane a disintegrin and metalloproteases (ADAMs). It plays a role in processes such as bone formation and retinal neovascularization, and importantly, its expression in human cancers correlates with disease stage and poor prognosis. Functionally, ADAM9 can cleave several transmembrane proteins, thereby shedding their ectodomains from the cell surface. Moreover, ADAM9 regulates cell behaviour by binding cell-surface receptors such as integrin and membrane-type matrix metalloproteases...
April 5, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29569114/the-phox-homology-px-domain
#10
Mintu Chandra, Brett M Collins
The phox-homology (PX) domain is a phosphoinositide-binding domain conserved in all eukaryotes and present in 49 human proteins. Proteins containing PX domains, many of which are also known as sorting nexins (SNXs), have a large variety of functions in membrane trafficking, cell signaling, and lipid metabolism in association with membranes of the secretory and endocytic system. In this review we discuss the structural basis for both canonical lipid interactions with the endosome-enriched lipid phosphatidylinositol-3-phosphate (PtdIns3P) as well as non-canonical lipids that promote membrane association...
March 23, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29563155/sorting-nexin-27-snx27-regulates-the-trafficking-and-activity-of-the-glutamine-transporter-asct2
#11
Zhe Yang, Jordan Follett, Markus C Kerr, Thomas Clairfeuille, Mintu Chandra, Brett M Collins, Rohan D Teasdale
Alanine-, serine-, cysteine-preferring transporter 2 (ASCT2, SLC1A5) is responsible for the uptake of glutamine into cells, a major source of cellular energy and a key regulator of mammalian target of rapamycin (mTOR) activation. Furthermore, ASCT2 expression has been reported in several human cancers, making it a potential target for both diagnostic and therapeutic purposes. Here we identify ASCT2 as a membrane-trafficked cargo molecule, sorted through a direct interaction with the PDZ domain of sorting nexin 27 (SNX27)...
May 4, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29530981/the-proximity-labeling-technique-bioid-identifies-sorting-nexin-6-as-a-member-of-the-insulin-like-growth-factor-1-igf1-igf1-receptor-pathway
#12
Akshay Bareja, Conrad P Hodgkinson, Erik Soderblom, Greg Waitt, Victor J Dzau
The insulin-like growth factor 1 receptor (IGF1R) is a receptor tyrosine kinase with critical roles in various biological processes. Recent results from clinical trials targeting IGF1R indicate that IGF1R signaling pathways are more complex than previously thought. Moreover, it has become increasingly clear that the function of many proteins can be understood only in the context of a network of interactions. To that end, we sought to profile IGF1R-protein interactions with the proximity-labeling technique BioID...
April 27, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29520003/phosphorylation-of-conserved-phosphoinositide-binding-pocket-regulates-sorting-nexin-membrane-targeting
#13
Marc Lenoir, Cansel Ustunel, Sandya Rajesh, Jaswant Kaur, Dimitri Moreau, Jean Gruenberg, Michael Overduin
Sorting nexins anchor trafficking machines to membranes by binding phospholipids. The paradigm of the superfamily is sorting nexin 3 (SNX3), which localizes to early endosomes by recognizing phosphatidylinositol 3-phosphate (PI3P) to initiate retromer-mediated segregation of cargoes to the trans-Golgi network (TGN). Here we report the solution structure of full length human SNX3, and show that PI3P recognition is accompanied by bilayer insertion of a proximal loop in its extended Phox homology (PX) domain. Phosphoinositide (PIP) binding is completely blocked by cancer-linked phosphorylation of a conserved serine beside the stereospecific PI3P pocket...
March 8, 2018: Nature Communications
https://www.readbyqxmd.com/read/29514873/multiplex-genomewide-association-analysis-of-breast-milk-fatty-acid-composition-extends-the-phenotypic-association-and-potential-selection-of-fads1-variants-to-arachidonic-acid-a-critical-infant-micronutrient
#14
Josyf C Mychaleckyj, Uma Nayak, E Ross Colgate, Dadong Zhang, Tommy Carstensen, Shahnawaz Ahmed, Tahmeed Ahmed, Alexander J Mentzer, Masud Alam, Beth D Kirkpatrick, Rashidul Haque, Abu Syed Golam Faruque, William A Petri
BACKGROUND: Breast milk is the sole nutrition source during exclusive breastfeeding, and polyunsaturated fatty acids (FAs) are critical micronutrients in infant physical and cognitive development. There has been no prior genomewide association study of breast milk, hence our objective was to test for genetic association with breast milk FA composition. METHODS: We measured the fractional composition of 26 individual FAs in breast milk samples from three cohorts totalling 1142 Bangladeshi mothers whose infants were genotyped on the Illumina MEGA chip and replicated on a custom Affymetrix 30K SNP array (n=616)...
March 7, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29478026/genome-wide-association-approach-identified-novel-genetic-predictors-of-heart-rate-response-to-%C3%AE-blockers
#15
Mohamed H Shahin, Daniela J Conrado, Daniel Gonzalez, Yan Gong, Maximilian T Lobmeyer, Amber L Beitelshees, Eric Boerwinkle, John G Gums, Arlene Chapman, Stephen T Turner, Rhonda M Cooper-DeHoff, Julie A Johnson
BACKGROUND: For many indications, the negative chronotropic effect of β-blockers is important to their efficacy, yet the heart rate (HR) response to β-blockers varies. Herein, we sought to use a genome-wide association approach to identify novel single nucleotide polymorphisms (SNPs) associated with HR response to β-blockers. METHODS AND RESULTS: We first performed 4 genome-wide association analyses for HR response to atenolol (a β1-adrenergic receptor blocker) as: (1) monotherapy or (2) add-on therapy, in 426 whites and 273 blacks separately from the PEAR (Pharmacogenomic Evaluation of Antihypertensive Responses) study...
February 24, 2018: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29463731/gibberellin-della-signaling-targets-the-retromer-complex-to-redirect-protein-trafficking-to-the-plasma-membrane
#16
Yuliya Salanenka, Inge Verstraeten, Christian Löfke, Kaori Tabata, Satoshi Naramoto, Matouš Glanc, Jiří Friml
The plant hormone gibberellic acid (GA) is a crucial regulator of growth and development. The main paradigm of GA signaling puts forward transcriptional regulation via the degradation of DELLA transcriptional repressors. GA has also been shown to regulate tropic responses by modulation of the plasma membrane incidence of PIN auxin transporters by an unclear mechanism. Here we uncovered the cellular and molecular mechanisms by which GA redirects protein trafficking and thus regulates cell surface functionality...
April 3, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29452354/alpha-synuclein-inhibits-snx3-retromer-mediated-retrograde-recycling-of-iron-transporters-in-s-cerevisiae-and-c-elegans-models-of-parkinson-s-disease
#17
Dhaval Patel, Chuan Xu, Sureshbabu Nagarajan, Zhengchang Liu, Wayne O Hemphill, Runhua Shi, Vladimir N Uversky, Guy A Caldwell, Kim A Caldwell, Stephan N Witt
We probed the role of alpha-synuclein (α-syn) in modulating sorting nexin 3 (Snx3)-retromer-mediated recycling of iron transporters in Saccharomyces cerevisiae and Caenorhabditis elegans. In yeast, the membrane-bound heterodimer Fet3/Ftr1 is the high affinity iron importer. Fet3 is a membrane-bound multicopper ferroxidase, whose ferroxidase domain is orthologous to human ceruloplasmin (Cp), that oxidizes external Fe+2 to Fe+3; the Fe+3 ions then channel through the Ftr1 permease into the cell. When the concentration of external iron is low (<1 µM), Fet3/Ftr1 is maintained on the plasma membrane by retrograde endocytic-recycling; whereas, when the concentration of external iron is high (>10 µM), Fet3/Ftr1 is endocytosed and shunted to the vacuole for degradation...
May 1, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29452206/snx10-mediates-alcohol-induced-liver-injury-and-steatosis-by-regulating-the-activation-of-chaperone-mediated-autophagy
#18
Yan You, Wan-Zhen Li, Sulin Zhang, Bin Hu, Yue-Xuan Li, Hai-Dong Li, Huan-Huan Tang, Qian-Wen Li, Yun-Yun Guan, Li-Xin Liu, Wei-Lian Bao, Xiaoyan Shen
BACKGROUND & AIMS: Alcoholic liver disease (ALD) is a major cause of morbidity and mortality worldwide. However, the cellular defense mechanisms underlying ALD are not well understood. Recent studies highlighted the involvement of chaperone-mediated autophagy (CMA) in regulating hepatic lipid metabolism. Sorting nexin (SNX)-10 has a regulatory function in endolysosomal trafficking and stabilisation. Here, we investigated the roles of SNX10 in CMA activation and in the pathogenesis of alcohol-induced liver injury and steatosis...
February 13, 2018: Journal of Hepatology
https://www.readbyqxmd.com/read/29444945/interaction-of-human-cytomegalovirus-tegument-proteins-ppul35-and-ppul35a-with-sorting-nexin-5-regulates-glycoprotein-b-gpul55-localization
#19
Gregor Maschkowitz, Sabine Gärtner, Heike Hofmann-Winkler, Helmut Fickenscher, Michael Winkler
Human cytomegalovirus (HCMV) is a widespread human pathogen that causes asymptomatic infection in healthy individuals but poses a serious threat to immunocompromised patients. During the late phase of HCMV infection, the viral capsid is transported to the cytoplasmic viral assembly center (cVAC), where it is enclosed by the tegument protein layer and the viral envelope. The cVAC consists of circularly arranged vesicles from the trans -Golgi and endosomal networks. The HCMV gene UL35 encodes ppUL35 and its shorter form, ppUL35A...
May 1, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29414832/overexpression-of-snx3-decreases-amyloid-%C3%AE-peptide-production-by-reducing-internalization-of-amyloid-precursor-protein
#20
Shaohua Xu, Saket M Nigam, Lennart Brodin
BACKGROUND: Sorting nexins (SNXs) have diverse functions in protein sorting and membrane trafficking. Recently, single-nucleotide polymorphisms in SNX3 were found to be associated with Alzheimer disease. However, it remains unknown whether SNX3 participates in amyloid (A)β peptide production. OBJECTIVE: To examine the role of SNX3 in Aβ production and APP processing. METHODS: The effect of increased expression of SNX3 was studied in HEK293T cells...
2018: Neuro-degenerative Diseases
keyword
keyword
53263
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"