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https://www.readbyqxmd.com/read/27911256/a-residue-located-at-the-junction-of-the-head-and-stalk-regions-of-measles-virus-fusion-protein-regulates-membrane-fusion-by-controlling-conformational-stability
#1
Yuto Satoh, Saeka Yonemori, Mitsuhiro Hirose, Hiroko Shogaki, Hiroshi Wakimoto, Yoshinori Kitagawa, Bin Gotoh, Tsuyoshi Shirai, Ken-Ichi Takahashi, Masae Itoh
The fusion (F) protein of measles virus (MV) performs refolding from the thermodynamically metastable prefusion form to the highly stable postfusion form via an activated unstable intermediate stage, to induce membrane fusion. Some amino acids involved in the fusion regulation cluster in the heptad repeat B (HR-B) domain of the stalk region, among which substitution of residue 465 by various amino acids revealed that fusion activity correlates well with its side chain length from the Cα (p<0.01) and van der Waals volume (p<0...
December 1, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27910991/structural-fingerprints-of-yielding-mechanisms-in-attractive-colloidal-gels
#2
Arman Boromand, Safa Jamali, João M Maia
Core-Modified Dissipative Particle Dynamics (CM-DPD) with a modified depletion potential and full hydrodynamics description is used to study non-equilibrium properties of colloidal gels with short range attraction potentials at an intermediate volume fraction (ϕ = 0.2) under start-up shear deformation. Full structural and rheological analysis using the stress fabric tensor complemented by bond number and bond distribution evolution under flow reveals that similarly to dilute colloidal gels, flow-induced anisotropy and strain-induced stretching of bonds are present during the first yielding transition...
December 2, 2016: Soft Matter
https://www.readbyqxmd.com/read/27910904/melanosome-transfer-to-keratinocyte-in-the-chicken-embryonic-skin-is-mediated-by-vesicle-release-associated-with-rho-regulated-membrane-blebbing
#3
Ryosuke Tadokoro, Hidetaka Murai, Ken-Ichiro Sakai, Takahiro Okui, Yasuhiro Yokota, Yoshiko Takahashi
During skin pigmentation in amniotes, melanin synthesized in the melanocyte is transferred to keratinocytes by a particle called the melanosome. Previous studies, mostly using dissociated cultured cells, have proposed several different models that explain how the melanosome transfer is achieved. Here, using a technique that labels the plasma membrane of melanocytes within a three-dimensional system that mimics natural tissues, we have visualized the plasma membrane of melanocytes with EGFP in chicken embryonic skin...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910850/the-guanine-nucleotide-exchange-factor-net1-facilitates-the-specification-of-dorsal-cell-fates-in-zebrafish-embryos-by-promoting-maternal-%C3%AE-catenin-activation
#4
Shi Wei, Miaomiao Dai, Zhaoting Liu, Yuanqing Ma, Hanqiao Shang, Yu Cao, Qiang Wang
Wnt/β-catenin signaling is essential for the initiation of dorsal-ventral patterning during vertebrate embryogenesis. Maternal β-catenin accumulates in dorsal marginal nuclei during cleavage stages, but its critical target genes essential for dorsalization are silent until mid-blastula transition (MBT). Here, we find that zebrafish net1, a guanine nucleotide exchange factor, is specifically expressed in dorsal marginal blastomeres after MBT, and acts as a zygotic factor to promote the specification of dorsal cell fates...
December 2, 2016: Cell Research
https://www.readbyqxmd.com/read/27910505/characterization-of-an-atomic-hydrogen-source-for-charge-exchange-experiments
#5
M A Leutenegger, P Beiersdorfer, G L Betancourt-Martinez, G V Brown, N Hell, R L Kelley, C A Kilbourne, E W Magee, F S Porter
We characterized the dissociation fraction of a thermal dissociation atomic hydrogen source by injecting the mixed atomic and molecular output of the source into an electron beam ion trap containing highly charged ions and recording the x-ray spectrum generated by charge exchange using a high-resolution x-ray calorimeter spectrometer. We exploit the fact that the charge exchange state-selective capture cross sections are very different for atomic and molecular hydrogen incident on the same ions, enabling a clear spectroscopic diagnostic of the neutral species...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910231/monitoring-the-reaction-mechanism-in-model-biogas-reforming-by-in-situ-transient-and-steady-state-drifts-measurements
#6
Luis F Bobadilla, Victoria Garcilaso, Miguel A Centeno, José A Odriozola
In this work, the reforming of model biogas reaction has been investigated on a Rh/MgAl2O4 catalyst. In situ transients and steady-state DRIFTS measurements were used to gain insights about the reaction mechanism involved in the activation of CH4 and CO2. It was found that the reaction proceeds through of an initial pathway in which methane and CO2 are both dissociated on Rh metallic sites, and additionally a bifunctional mechanism in which methane is activated on Rh sites and CO2 is activated on the basic sites of the support surface via formate intermediate by H-assisted CO2 decomposition...
December 1, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27909710/intracellular-distribution-and-stability-of-a-luminescent-rhenium-i-tricarbonyl-tetrazolato-complex-using-epifluorescence-microscopy-in-conjunction-with-x-ray-fluorescence-imaging
#7
J L Wedding, H H Harris, C A Bader, S E Plush, R Mak, M Massi, D A Brooks, B Lai, S Vogt, M V Werrett, P V Simpson, B W Skelton, S Stagni
Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques...
December 2, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27909311/insights-into-the-phosphoryl-transfer-mechanism-of-human-ubiquitous-mitochondrial-creatine-kinase
#8
Quanjie Li, Shuai Fan, Xiaoyu Li, Yuanyuan Jin, Weiqing He, Jinming Zhou, Shan Cen, ZhaoYong Yang
Human ubiquitous mitochondrial creatine kinase (uMtCK) is responsible for the regulation of cellular energy metabolism. To investigate the phosphoryl-transfer mechanism catalyzed by human uMtCK, in this work, molecular dynamic simulations of uMtCK∙ATP-Mg(2+)∙creatine complex and quantum mechanism calculations were performed to make clear the puzzle. The theoretical studies hereof revealed that human uMtCK utilizes a two-step dissociative mechanism, in which the E227 residue of uMtCK acts as the catalytic base to accept the creatine guanidinium proton...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27909098/reversible-disruption-of-neuronal-mitochondria-by-ischemic-and-traumatic-injury-revealed-by-quantitative-two-photon-imaging-in-the-neocortex-of-anesthetized-mice
#9
Mikhail Kislin, Jeremy Sword, Ioulia V Fomitcheva, Deborah Croom, Evgeny Pryazhnikov, Eero Lihavainen, Dmytro Toptunov, Heikki Rauvala, Andre S Ribeiro, Leonard Khiroug, Sergei A Kirov
: Mitochondria play a variety of functional roles in cortical neurons, from metabolic support and neuroprotection to the release of cytokines that trigger apoptosis. In dendrites, mitochondrial structure is closely linked to their function, and fragmentation (fission) of the normally elongated mitochondria indicates loss of their function under such pathological conditions as stroke and brain trauma. Using in vivo two-photon microscopy in mouse brain, we quantified mitochondrial fragmentation in a full spectrum of cortical injuries ranging from severe to mild...
December 1, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27908787/fractionating-the-anterior-temporal-lobe-mvpa-reveals-differential-responses-to-input-and-conceptual-modality
#10
Charlotte Murphy, Shirley-Ann Rueschemeyer, David Watson, Theodoros Karapanagiotidis, Jonathan Smallwood, Elizabeth Jefferies
Words activate cortical regions in accordance with their modality of presentation (i.e., written vs. spoken), yet there is a long-standing debate about whether patterns of activity in any specific brain region capture modality-invariant conceptual information. Deficits in patients with semantic dementia highlight the anterior temporal lobe (ATL) as an amodal store of semantic knowledge but these studies do not permit precise localisation of this function. The current investigation used multiple imaging methods in healthy participants to examine functional dissociations within ATL...
November 28, 2016: NeuroImage
https://www.readbyqxmd.com/read/27908240/new-fluorescent-macrolide-derivatives-for-studying-interactions-of-antibiotics-and-their-analogs-with-the-ribosomal-exit-tunnel
#11
A G Tereshchenkov, A V Shishkina, V V Karpenko, V A Chertkov, A L Konevega, P S Kasatsky, A A Bogdanov, N V Sumbatyan
Novel fluorescent derivatives of macrolide antibiotics related to tylosin bearing rhodamine, fluorescein, Alexa Fluor 488, BODIPY FL, and nitrobenzoxadiazole (NBD) residues were synthesized. The formation of complexes of these compounds with 70S E. coli ribosomes was studied by measuring the fluorescence polarization depending on the ribosome amount at constant concentration of the fluorescent substance. With the synthesized fluorescent tylosin derivatives, the dissociation constants for ribosome complexes with several known antibiotics and macrolide analogs previously obtained were determined...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27908104/short-and-long-range-corrected-hybrid-density-functionals-with-the-d3-dispersion-corrections
#12
Chih-Wei Wang, Kerwin Hui, Jeng-Da Chai
We propose a short- and long-range corrected (SLC) hybrid scheme employing 100% Hartree-Fock exchange at both zero and infinite interelectronic distances, wherein three SLC hybrid density functionals with the D3 dispersion corrections (SLC-LDA-D3, SLC-PBE-D3, and SLC-B97-D3) are developed. SLC-PBE-D3 and SLC-B97-D3 are shown to be accurate for a very diverse range of applications, such as core ionization and excitation energies, thermochemistry, kinetics, noncovalent interactions, dissociation of symmetric radical cations, vertical ionization potentials, vertical electron affinities, fundamental gaps, and valence, Rydberg, and long-range charge-transfer excitation energies...
November 28, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27907818/reactive-dissociative-experiences-in-response-to-acute-increases-in-shame-feelings
#13
Martin J Dorahy, Hadyn McKendry, Amber Scott, Kumar Yogeeswaran, Andy Martens, Donncha Hanna
Studies have not examined if a direct causal relationship exists between shame and dissociation. The current research examined whether increases in dissociation were evident following exposure to acute shame feelings induced via narrative scripts of shame-evoking situations. Following Gilbert's (1998) differentiation between external and internal shame, participants heard shame-inducing or emotionally neutral stories in conditions designed to heighten (1) external shame, (2) internal shame or (3) general shame...
November 15, 2016: Behaviour Research and Therapy
https://www.readbyqxmd.com/read/27907091/real-time-fluorescence-measurements-of-ros-and-ca2-in-ischemic-reperfused-rat-hearts-detectable-increases-occur-only-after-mitochondrial-pore-opening-and-are-attenuated-by-ischemic-preconditioning
#14
Tatyana Andrienko, Philippe Pasdois, Andreas Rossbach, Andrew P Halestrap
Mitochondrial permeability transition pore (mPTP) opening is critical for ischemia / reperfusion (I/R) injury and is associated with increased [Ca2+] and reactive oxygen species (ROS). Here we employ surface fluorescence to establish the temporal sequence of these events in beating perfused hearts subject to global I/R. A bespoke fluorimeter was used to synchronously monitor surface fluorescence and reflectance of Langendorff-perfused rat hearts at multiple wavelengths, with simultaneous measurements of hemodynamic function...
2016: PloS One
https://www.readbyqxmd.com/read/27907075/erp-correlates-of-encoding-success-and-encoding-selectivity-in-attention-switching
#15
Franziska R Richter, Nick Yeung
Long-term memory encoding depends critically on effective processing of incoming information. The degree to which participants engage in effective encoding can be indexed in electroencephalographic (EEG) data by studying event-related potential (ERP) subsequent memory effects. The current study investigated ERP correlates of memory success operationalised with two different measures-memory selectivity and global memory-to assess whether previously observed ERP subsequent memory effects reflect focused encoding of task-relevant information (memory selectivity), general encoding success (global memory), or both...
2016: PloS One
https://www.readbyqxmd.com/read/27906395/regioselective-plasmonic-nano-assemblies-for-bimodal-sub-femtomolar-dopamine-detection
#16
Fengli Gao, Liqiang Liu, Gang Cui, Liguang Xu, Xiaoling Wu, Hua Kuang, Chuanlai Xu
Reliable and ultrasensitive quantification of dopamine (DA) is essential in the precise diagnotherapy of neurological diseases. In this study, dual mode counterpropagating-responsive gold@silver nanoparticle-gold nanorod (Au@AgNP-AuNR) nano-assemblies were fabricated for the precise quantification of DA. The plasmonic Au@AgNP-AuNR assemblies possessed high surface-enhanced Raman scattering (SERS) activity and strong fluorescence quenching, due to the prominent electromagnetic enhancement between the hotspots of the assemblies...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27906103/progressive-impairment-of-cav1-1-function-in-the-skeletal-muscle-of-mice-expressing-a-mutant-type-1-cu-zn-superoxide-dismutase-g93a-linked-to-amyotrophic-lateral-sclerosis
#17
Donald Beqollari, Christin F Romberg, Gabriella Dobrowolny, Martina Martini, Andrew A Voss, Antonio Musarò, Roger A Bannister
BACKGROUND: Amyotrophic lateral sclerosis (ALS) is an adult-onset neurodegenerative disorder that is typically fatal within 3-5 years of diagnosis. While motoneuron death is the defining characteristic of ALS, the events that underlie its pathology are not restricted to the nervous system. In this regard, ALS muscle atrophies and weakens significantly before presentation of neurological symptoms. Since the skeletal muscle L-type Ca(2+) channel (CaV1.1) is a key regulator of both mass and force, we investigated whether CaV1...
June 23, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27905597/mechanism-for-the-enhanced-oxygen-reduction-reaction-of-la0-6sr0-4co0-2fe0-8o3-%C3%AE-by-strontium-carbonate
#18
Mei Li, Zhongti Sun, Wenqiang Yang, Tao Hong, Zhesheng Zhu, Yanxiang Zhang, Xiaojun Wu, Changrong Xia
Strontium doped lanthanum cobalt ferrite (LSCF) is a widely applied electrocatalyst for the oxygen reduction reaction (ORR) in solid-oxide fuel cells (SOFCs) operated at intermediate temperatures. Sr surface segregation in long-term operation has been reported to have contradicting effects that either degrade or improve the reaction. Thus, it is critical to understand the mechanism of surface Sr compounds on ORR kinetics. This work aims to verify the effect and propose the mechanism by decorating SrCO3 nanoparticles using the infiltration method...
December 1, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27905517/g4ipdb-a-database-for-g-quadruplex-structure-forming-nucleic-acid-interacting-proteins
#19
Subodh Kumar Mishra, Arpita Tawani, Amit Mishra, Amit Kumar
Nucleic acid G-quadruplex structure (G4) Interacting Proteins DataBase (G4IPDB) is an important database that contains detailed information about proteins interacting with nucleic acids that forms G-quadruplex structures. G4IPDB is the first database that provides comprehensive information about this interaction at a single platform. This database contains more than 200 entries with details of interaction such as interacting protein name and their synonyms, their UniProt-ID, source organism, target name and its sequences, ∆Tm, binding/dissociation constants, protein gene name, protein FASTA sequence, interacting residue in protein, related PDB entries, interaction ID, graphical view, PMID, author's name and techniques that were used to detect their interactions...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27905406/differential-roles-for-l-type-calcium-channel-subtypes-in-alcohol-dependence
#20
Stefanie Uhrig, David Vandael, Andrea Marcantoni, Nina Dedic, Ainhoa Bilbao, Miriam A Vogt, Natalie Hirth, Laura Broccoli, Rick E Bernardi, Kai Schönig, Peter Gass, Dusan Bartsch, Rainer Spanagel, Jan M Deussing, Wolfgang H Sommer, Emilio Carbone, Anita C Hansson
It has previously been shown that the inhibition of L-type calcium channels (LTCCs) decreases alcohol consumption, although the contribution of the central LTCC subtypes Cav1.2 and Cav1.3 remains unknown. Here, we determined changes in Cav1.2 (Cacna1c) and Cav1.3 (Cacna1d) mRNA and protein expression in alcohol dependent rats during protracted abstinence and naïve controls using in situ hybridization and Western Blot analysis. Functional validation was obtained by electrophysiological recordings of calcium currents in dissociated hippocampal pyramidal neurons...
December 1, 2016: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
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