keyword
MENU ▼
Read by QxMD icon Read
search

Memory Stem T-Cells

keyword
https://www.readbyqxmd.com/read/29664572/ilc2-memory-recollection-of-previous-activation
#1
REVIEW
Itziar Martinez-Gonzalez, Maryam Ghaedi, Catherine A Steer, Laura Mathä, Eric Vivier, Fumio Takei
Immunological memory, traditionally thought to belong to T and B cells, has now been extended to innate lymphocytes, including NK cells and ILC2s, myeloid cells such as macrophages, also termed "trained immunity" and more recently to epithelial stem cells. In this review, we discuss the mechanisms underlying memory generation on ILC2s and speculate about their potential role in human allergic diseases, such as asthma. Moreover, we examine the relevance of the spontaneous ILC2 activation in the lung during the neonatal period in order to efficiently respond to stimuli later in life...
May 2018: Immunological Reviews
https://www.readbyqxmd.com/read/29660266/role-of-stem-cell-like-memory-t-cells-in-systemic-lupus-erythematosus
#2
Ye Ji Lee, Ji Ah Park, Hyunmi Kwon, Youn Soo Choi, Kyeong Cheon Jung, Seong Hoe Park, Eun Bong Lee
OBJECTIVE: Stem cell-like memory T (TSCM ) cells are long-lived memory T cells that have multipotent capacity to differentiate into different subsets. However, the role of TSCM cells in autoimmune diseases remains unclear. Here, we performed phenotypic studies to identify TSCM cells in patients suffering from systemic lupus erythematosus (SLE). METHODS: CD4+ and CD8+ TSCM cells were identified in SLE patients and healthy controls (HCs). In vitro culture systems, CD4+ TSCM cells were induced to differentiate into subsets of T cells, including follicular helper T (TFH ) cells and cytokine production pattern was measured after stimulation...
April 16, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29605883/induction-of-a-central-memory-and-stem-cell-memory-phenotype-in-functionally-active-cd4-and-cd8-car-t-cells-produced-in-an-automated-good-manufacturing-practice-system-for-the-treatment-of-cd19-acute-lymphoblastic-leukemia
#3
Franziska Blaeschke, Dana Stenger, Theresa Kaeuferle, Semjon Willier, Ramin Lotfi, Andrew Didier Kaiser, Mario Assenmacher, Michaela Döring, Judith Feucht, Tobias Feuchtinger
Relapsed/refractory B-precursor acute lymphoblastic leukemia (pre-B ALL) remains a major therapeutic challenge. Chimeric antigen receptor (CAR) T cells are promising treatment options. Central memory T cells (Tcm) and stem cell-like memory T cells (Tscm) are known to promote sustained proliferation and persistence after T-cell therapy, constituting essential preconditions for treatment efficacy. Therefore, we set up a protocol for anti-CD19 CAR T-cell generation aiming at high Tcm/Tscm numbers. 100 ml peripheral blood from pediatric pre-B ALL patients was processed including CD4+ /CD8+ -separation, T-cell activation with modified anti-CD3/-CD28 reagents and transduction with a 4-1BB-based second generation CAR lentiviral vector...
March 31, 2018: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/29593456/robust-production-of-cytomegalovirus-pp65-specific-t-cells-using-a-fully-automated-ifn-%C3%AE-cytokine-capture-system
#4
Nayoun Kim, Young-Sun Nam, Keon-Il Im, Jung-Yeon Lim, Young-Woo Jeon, Yunejin Song, Jong Wook Lee, Seok-Goo Cho
Background: Cytomegalovirus(CMV)-related diseases are a serious cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). CMV-specific cytotoxic T lymphocytes (CMV-CTLs) have been reported as an alternative to antiviral drugs that provide long-term CMV-specific immunity without major side effects. However, their application has been limited by the prolonged manufacturing process required. Methods: In this study, we applied the IFN-γ cytokine capture system (CCS) using the fully automated CliniMACS Prodigy device for rapid production of CMV-CTLs, which may be applicable in clinically urgent CMV-related diseases...
January 2018: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/29568298/trying-to-see-the-forest-through-the-trees-deciphering-the-nature-of-memory-immunity-to-mycobacterium-tuberculosis
#5
REVIEW
Ian M Orme, Marcela I Henao-Tamayo
The purpose of vaccination against tuberculosis and other diseases is to establish a heightened state of acquired specific resistance in which the memory immune response is capable of mediating an accelerated and magnified expression of protection to the pathogen when this is encountered at a later time. In the earliest studies in mice infected with Mycobacterium tuberculosis , memory immunity and the cells that express this were definable both in terms of kinetics of emergence, and soon thereafter by the levels of expression of markers including CD44, CD62L, and the chemokine receptor CCR7, allowing the identification of effector memory and central memory T cell subsets...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29562175/generation-of-tumor-antigen-specific-ipsc-derived-thymic-emigrants-using-a-3d-thymic-culture-system
#6
Raul Vizcardo, Nicholas D Klemen, S M Rafiqul Islam, Devikala Gurusamy, Naritaka Tamaoki, Daisuke Yamada, Haruhiko Koseki, Benjamin L Kidder, Zhiya Yu, Li Jia, Amanda N Henning, Meghan L Good, Marta Bosch-Marce, Takuya Maeda, Chengyu Liu, Zied Abdullaev, Svetlana Pack, Douglas C Palmer, David F Stroncek, Fumito Ito, Francis A Flomerfelt, Michael J Kruhlak, Nicholas P Restifo
Induced pluripotent stem cell (iPSC)-derived T cells may provide future therapies for cancer patients, but those generated by current methods, such as the OP9/DLL1 system, have shown abnormalities that pose major barriers for clinical translation. Our data indicate that these iPSC-derived CD8 single-positive T cells are more like CD4+ CD8+ double-positive T cells than mature naive T cells because they display phenotypic markers of developmental arrest and an innate-like phenotype after stimulation. We developed a 3D thymic culture system to avoid these aberrant developmental fates, generating a homogeneous subset of CD8αβ+ antigen-specific T cells, designated iPSC-derived thymic emigrants (iTEs)...
March 20, 2018: Cell Reports
https://www.readbyqxmd.com/read/29545802/potential-beneficial-effects-of-cytomegalovirus-infection-after-transplantation
#7
REVIEW
Nicolle H R Litjens, Lotte van der Wagen, Jurgen Kuball, Jaap Kwekkeboom
Cytomegalovirus (CMV) infection can cause significant complications after transplantation, but recent emerging data suggest that CMV may paradoxically also exert beneficial effects in two specific allogeneic transplant settings. These potential benefits have been underappreciated and are therefore highlighted in this review. First, after allogeneic hematopoietic stem cell transplantation (HSCT) for acute myeloid leukemia (AML) using T-cell and natural killer (NK) cell-replete grafts, CMV reactivation is associated with protection from leukemic relapse...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29545791/functional-antigen-specific-stem-cell-memory-t-scm-cd4-t-cells-are-induced-by-human-mycobacterium-tuberculosis-infection
#8
Cheleka A M Mpande, One B Dintwe, Munyaradzi Musvosvi, Simbarashe Mabwe, Nicole Bilek, Mark Hatherill, Elisa Nemes, Thomas J Scriba
Background: Maintenance of long-lasting immunity is thought to depend on stem cell memory T cells (TSCM ), which have superior self-renewing capacity, longevity and proliferative potential compared with central memory (TCM ) or effector (TEFF ) T cells. Our knowledge of TSCM derives primarily from studies of virus-specific CD8+ TSCM . We aimed to determine if infection with Mycobacterium tuberculosis ( M. tb ), the etiological agent of tuberculosis, generates antigen-specific CD4+ TSCM and to characterize their functional ontology...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29506985/detection-and-characterization-of-cd8-autoreactive-memory-stem-t-cells-in-patients-with-type-1-diabetes
#9
Debora Vignali, Elisa Cantarelli, Carlotta Bordignon, Adriana Canu, Antonio Citro, Andrea Annoni, Lorenzo Piemonti, Paolo Monti
Stem memory T cells (Tscm) constitute the earliest developmental stage of memory T cells, displaying stem cell-like properties such as self-renewal capacity. Their superior immune reconstitution potential has sparked interest in cancer immune-therapy, vaccine development and immune reconstitution, whereas their role in autoimmunity is largely unexplored. Here we show that autoreactive CD8+ Tscm specific for β cell antigens GAD65, insulin and IGRP are present in patients with type 1 diabetes (T1D). In vitro, generation of autoreactive Tscm from naïve precursors required the presence of the homeostatic cytokine interleukin-7 (IL-7)...
March 5, 2018: Diabetes
https://www.readbyqxmd.com/read/29500416/sipa1-deficiency-unleashes-a-host-immune-mechanism-eradicating-chronic-myelogenous-leukemia-initiating-cells
#10
Yan Xu, Satoshi Ikeda, Kentaro Sumida, Ryusuke Yamamoto, Hiroki Tanaka, Nagahiro Minato
Chronic myelogenous leukemia (CML) caused by hematopoietic stem cells expressing the Bcr-Abl fusion gene may be controlled by Bcr-Abl tyrosine kinase inhibitors (TKIs). However, CML-initiating cells are resistant to TKIs and may persist as minimal residual disease. We demonstrate that mice deficient in Sipa1, which encodes Rap1 GTPase-activating protein, rarely develop CML upon transfer of primary hematopoietic progenitor cells (HPCs) expressing Bcr-Abl, which cause lethal CML disease in wild-type mice. Resistance requires both T cells and nonhematopoietic cells...
March 2, 2018: Nature Communications
https://www.readbyqxmd.com/read/29500069/generation-of-alloreactivity-reduced-donor-lymphocyte-products-retaining-memory-function-by-fully-automatic-depletion-of-cd45ra-positive-cells
#11
Nina Müller, Katharina Landwehr, Kirsten Langeveld, Joanna Stenzel, Walter Pouwels, Menno A W G van der Hoorn, Erhard Seifried, Halvard Bonig
BACKGROUND AIMS: For patients needing allogeneic stem cell transplantation but lacking a major histocompatibility complex (MHC)-matched donor, haplo-identical (family) donors may be an alternative. Stringent T-cell depletion required in these cases to avoid lethal graft-versus-host disease (GVHD) can delay immune reconstitution, thus impairing defense against virus reactivation and attenuating graft-versus-leukemia (GVL) activity. Several groups reported that GVHD is caused by cells residing within the naive (CD45RA+ ) T-cell compartment and proposed use of CD45RA-depleted donor lymphocyte infusion (DLI) to accelerate immune reconstitution...
February 28, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29498435/dental-follicle-mesenchymal-stem-cells-downregulate-th2-mediated-immune-response-in-asthmatic-patients-mononuclear-cells
#12
Deniz Genç, Noushin Zibandeh, Ercan Nain, Muazzez Gökalp, Ahmet Oğuzhan Özen, Mehmet Kamil Göker, Tunç Akkoç
BACKGROUND: Asthma is a chronic inflammatory disease in which inflammatory responses have the polarization of CD4+ T cells to Th2 cells. Dental follicle mesenchymal stem cells (DFSCs) have strong anti-inflammatory properties comparable to other mesenchymal stem cells. OBJECTIVE: We investigated the immunomodulatory effects of DFSCs on CD4+ T helper cell responses of asthmatic patients and compared the results with those obtained with asthmatic subjects on immunotherapy and with healthy individuals...
March 2, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29489900/characterization-and-clinical-enrichment-of-hla-c-07-02-restricted-cytomegalovirus-specific-cd8-t-cells
#13
Fabian Schlott, Dominik Steubl, Stefanie Ameres, Andreas Moosmann, Stefan Dreher, Uwe Heemann, Volker Hösel, Dirk H Busch, Michael Neuenhahn
Human Cytomegalovirus (CMV) reactivation remains a major source of morbidity in patients after solid organ and hematopoietic stem cell transplantation (HSCT). Adoptive T cell therapy (ACT) with CMV-specific T cells is a promising therapeutic approach for HSCT recipients, but might be counteracted by CMV's immune evasion strategies. HLA-C*07:02 is less susceptible to viral immune evasion suggesting HLA-C*07:02-restricted viral epitopes as promising targets for ACT. For a better understanding of HLA-C*07:02-restricted CMV-specific T cells we used recently generated reversible HLA-C*07:02/IE-1 multimers (Streptamers) recognizing a CMV-derived Immediate-Early-1 (IE-1) epitope and analyzed phenotypic and functional T cell characteristics...
2018: PloS One
https://www.readbyqxmd.com/read/29487057/human-herpesvirus-6-viremia-affects-t-cell-reconstitution-after-allogeneic-hematopoietic-stem-cell-transplantation
#14
Coco de Koning, Rick Admiraal, Stefan Nierkens, Jaap Jan Boelens
Human herpesvirus 6 (HHV6) viremia is a common cause of morbidity following allogeneic hematopoietic cell transplantation (HCT). We previously associated T-cell reconstitution with HHV6 viremia. Here, we investigated whether HHV6 viremia affects T-cell reconstitution after HCT in a time-dependent retrospective analysis. We included 273 pediatric patients (0.1-22.7 years; median follow-up, 58 months) receiving a first HCT between 2004 and 2014. HHV6 was screened weekly in plasma via polymerase chain reaction and occurred in 79 patients (29%) at a median time of 19 days after transplant...
February 27, 2018: Blood Advances
https://www.readbyqxmd.com/read/29479065/pi3k-orchestration-of-the-in-vivo-persistence-of-chimeric-antigen-receptor-modified-t-cells
#15
Wenting Zheng, Carol E O'Hear, Rajshekhar Alli, Jacob H Basham, Hossam A Abdelsamed, Lance E Palmer, Lindsay L Jones, Ben Youngblood, Terrence L Geiger
In vivo persistence of chimeric antigen receptor (CAR)-modified T cells correlates with therapeutic efficacy, yet CAR-specific factors that support persistence are not well resolved. Using a CD33-specific CAR in an acute myeloid leukemia (AML) model, we show how CAR expression alters T cell differentiation in a ligand independent manner. Ex vivo expanded CAR-T cells demonstrated decreased naïve and stem memory populations and increased effector subsets relative to vector-transduced control cells. This was associated with reduced in vivo persistence...
February 2, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/29475789/a-new-approach-to-car-t-cell-gene-engineering-and-cultivation-using-piggybac-transposon-in-the-presence-of-il-4-il-7-and-il-21
#16
Pavlína Ptáčková, Jan Musil, Martin Štach, Petr Lesný, Šárka Němečková, Vlastimil Král, Milan Fábry, Pavel Otáhal
BACKGROUND AIMS: Clinical-grade chimeric antigenic receptor (CAR)19 T cells are routinely manufactured by lentiviral/retroviral (LV/RV) transduction of an anti-CD3/CD28 activated T cells, which are then propagated in a culture medium supplemented with interleukin (IL)-2. The use of LV/RVs for T-cell modification represents a manufacturing challenge due to the complexity of the transduction approach and the necessity of thorough quality control. METHODS: We present here a significantly improved protocol for CAR19 T-cell manufacture that is based on the electroporation of peripheral blood mononuclear cells with plasmid DNA encoding the piggyBac transposon/transposase vectors and their cultivation in the presence of cytokines IL-4, IL-7 and IL-21...
February 20, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29472350/memory-cd8-t-cells-support-the-maintenance-of-hematopoietic-stem-cells-in-the-bone-marrow
#17
Sulima Geerman, Giso Brasser, Sudeep Bhushal, Fiamma Salerno, Natasja A Kragten, Mark Hoogenboezem, Gerald de Haan, Monika C Wolkers, María Fernanda Pascutti, Martijn A Nolte
No abstract text is available yet for this article.
February 22, 2018: Haematologica
https://www.readbyqxmd.com/read/29466292/targeting-leukemia-stem-cells-in-the-bone-marrow-niche
#18
REVIEW
Sarah K Tasian, Martin Bornhäuser, Sergio Rutella
Abst ract: The bone marrow (BM) niche encompasses multiple cells of mesenchymal and hematopoietic origin and represents a unique microenvironment that is poised to maintain hematopoietic stem cells. In addition to its role as a primary lymphoid organ through the support of lymphoid development, the BM hosts various mature lymphoid cell types, including naïve T cells, memory T cells and plasma cells, as well as mature myeloid elements such as monocyte/macrophages and neutrophils, all of which are crucially important to control leukemia initiation and progression...
February 21, 2018: Biomedicines
https://www.readbyqxmd.com/read/29409351/cd33-specific-chimeric-antigen-receptor-t-cells-with-different-costimulators-showed-potent-anti-leukemia-efficacy-and-different-phenotype
#19
Saisai Li, Zhongfei Tao, Yingxi Xu, Jia Liu, Na An, Ying Wang, Haiyan Xing, Zheng Tian, Kejing Tang, Xiaolong Liao, Qing Rao, Min Wang, Jianxiang Wang
Acute myeloid leukemia (AML) is a kind of a malignant hematologic tumor caused by uncontrolled repopulation of myeloid hematopoietic stem cells (HSCs). Current therapeutic effects for AML patients are unsatisfactory. Especially relapsed and refractory AML still have poor prognosis. T cell modified by chimeric antigen receptor (CAR) is an immunotherapeutic strategy for malignancies, which has a broad developing prospect. Most of AML cells overexpress the myeloid antigen CD33. Therefore, CD33-specific CAR-T cells with different costimulators (CD28, 4-1BB or both, referred to as CD33 28z...
February 6, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29408330/new-human-combined-immunodeficiency-caused-by-interferon-regulatory-factor-4-irf4-deficiency-inherited-by-uniparental-isodisomy
#20
María Bravo García-Morato, Francisco Javier Aracil Santos, Alejandro Contreras Briones, Alfonso Blázquez Moreno, Ángela Del Pozo Maté, Ángeles Domínguez-Soto, María José Beato Merino, Lucía Del Pino Molina, Juan Torres Canizales, Ana Victoria Marin, Elena Vallespín García, Marta Feito Rodríguez, Diego Plaza López Sabando, Anaïs Jiménez-Reinoso, Yasmina Mozo Del Castillo, Francisco José Sanz Santaeufemia, Raúl de Lucas-Laguna, Paula P Cárdenas, Laura Casamayor Polo, María Coronel Díaz, Mar Valés-Gómez, Ernesto Roldán Santiago, Antonio Ferreira Cerdán, Julián Nevado Blanco, Ángel L Corbí, Hugh T Reyburn, José Ramón Regueiro, Eduardo López-Granados, Rebeca Rodríguez Pena
No abstract text is available yet for this article.
February 28, 2018: Journal of Allergy and Clinical Immunology
keyword
keyword
53226
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"