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https://www.readbyqxmd.com/read/29040878/c-phycocyanin-supplementation-during-in%C3%A2-vitro-maturation-enhances-pre-implantation-developmental-competence-of-parthenogenetic-and-cloned-embryos-in-pigs
#1
Shuang Liang, Jing Guo, Yong Xun Jin, Bao Yuan, Jia-Bao Zhang, Nam-Hyung Kim
C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the current study was to explore the effects of C-PC on the maturation of porcine oocytes and subsequent developmental competence after parthenogenetic activation and somatic cell nuclear transfer (SCNT) as well as the underlying mechanisms. There was no significant improvement in nuclear maturation rates between the control and C-PC supplementation groups (1, 3, 5, 10 μg/mL)...
September 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/29030661/identification-of-the-major-histocompatibility-complex-class-ii-dm-and-do-alleles-in-a-cohort-of-northern-pig-tailed-macaques-macaca-leonina
#2
Xiao-Dong Lian, Xi-He Zhang, Zheng-Xi Dai, Yong-Tang Zheng
The northern pig-tailed macaque (Macaca leonina) has been considered as an independent species from the pig-tailed macaque group. We have previously reported that this species macaque has the potential to be a useful animal model in HIV/AIDS pathogenesis and vaccine studies due to its susceptibility to HIV-1. To develop this animal into a potential HIV/AIDS model, we have studied the classical MHC genes of this animal. In this study, the non-classical MHC genes Malo-DM and Malo-DO alleles were first characterized by sequencing and cloning in 12 unrelated northern pig-tailed macaques...
October 13, 2017: Immunogenetics
https://www.readbyqxmd.com/read/28993973/crispr-cas9-mediated-knockout-of-myostatin-in-chinese-indigenous-erhualian-pigs
#3
Kankan Wang, Xiaochun Tang, Zicong Xie, Xiaodong Zou, Mengjing Li, Hongming Yuan, Nannan Guo, Hongsheng Ouyang, Huping Jiao, Daxin Pang
CRISPR/Cas9 has emerged as one of the most popular genome editing tools due to its simple design and high efficiency in multiple species. Myostatin (MSTN) negatively regulates skeletal muscle growth and mutations in myostatin cause double-muscled phenotype in various animals. Here, we generated myostatin mutation in Erhualian pigs using a combination of CRISPR/Cas9 and somatic cell nuclear transfer. The protein level of myostatin precursor decreased dramatically in mutant cloned piglets. Unlike myostatin knockout Landrace, which often encountered health issues and died shortly after birth, Erhualian pigs harboring homozygous mutations were viable...
October 9, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28971562/aging-adult-porcine-fibroblasts-can-support-nuclear-transfer-and-transcription-factor-mediated-reprogramming
#4
Xia Li, Pengfei Zhang, Shaoshuai Jiang, Biao Ding, Xiaoyuan Zuo, Yunsheng Li, Zubing Cao, Yunhai Zhang
Somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs) technology are two classical reprogramming methods. Donor cell types can affect the reprogramming results in the above two methods. We here used porcine embryonic fibroblasts (PEFs) and adult porcine ear skin fibroblasts (APEFs) and adipose-derived stem cells (ADSCs) as donor cells for SCNT and source cells for iPSCs to study their in vitro developmental capability and colony-formation efficiency, respectively. For SCNT, fusion and cleavage rate has no significant difference among PEFs, ADSCs and APEFs...
October 3, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28966141/distribution-of-the-pig-gastrin-releasing-peptide-receptor-and-the-effect-of-grp-on-porcine-leydig-cells
#5
Zhiyu Ma, Ying Zhang, Juan Su, Xiang Li, Sheng Yang, Wenna Qiao, Chuan Suo, Zhihai Lei
Gastrin-releasing peptide (GRP) is a mammalian bombesin (BN)-like peptide which plays a role in a number of important physiological functions via its receptor (gastrin-releasing peptide receptor, GRPR) in most animals. However, little is known about the gene encoding GRPR and its functions (especially reproduction) in pigs. In this study, we first cloned and analyzed the pig GRPR cDNA. Then we systematically investigated the expression levels of GRPR mRNA by relative real-time PCR (RT-PCR), and analyzed the distribution of the GRPR protein in pig tissues via immunohistochemistry (IHC)...
September 28, 2017: Peptides
https://www.readbyqxmd.com/read/28966132/determination-of-complete-sequence-information-of-the-human-abo-blood-group-orthologous-gene-in-pigs-and-breed-difference-in-blood-type-frequencies
#6
Min-Kyeung Choi, Minh Thong Le, Hyesun Cho, Joori Yum, Mingue Kang, Hyuk Song, Jin-Hoi Kim, Hak Jae Chung, Kwonho Hong, Chankyu Park
The sequence information of the genomic form of the human ABO blood group orthologous gene (erythrocyte antigen A, EAA) is not complete in pigs. Therefore, we cloned and characterized the nucleotide sequence of EAA intron 7, which is critical to understand genetic difference between A and 0 blood groups in pigs, covering complete genomic sequence information of EAA excluding a ~560bp unsequencible gap. We also analyzed genetic polymorphisms within EAA intron 7 and exon 8. We found difference in A0 blood group frequencies among pig breeds...
September 28, 2017: Gene
https://www.readbyqxmd.com/read/28899413/evolution-and-genome-specialization-of-brucella-suis-biovar-2-iberian-lineages
#7
Ana Cristina Ferreira, Rogério Tenreiro, Maria Inácia Corrêa de Sá, Ricardo Dias
BACKGROUND: Swine brucellosis caused by B. suis biovar 2 is an emergent disease in domestic pigs in Europe. The emergence of this pathogen has been linked to the increase of extensive pig farms and the high density of infected wild boars (Sus scrofa). In Portugal and Spain, the majority of strains share specific molecular characteristics, which allowed establishing an Iberian clonal lineage. However, several strains isolated from wild boars in the North-East region of Spain are similar to strains isolated in different Central European countries...
September 12, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28893253/a-novel-swine-model-for-evaluation-of-dyslipidemia-and-atherosclerosis-induced-by-human-cetp-overexpression
#8
Tao Chen, Meng Sun, Jia-Qiang Wang, Jin-Jin Cui, Zhong-Hua Liu, Bo Yu
BACKGROUND: The mechanism of cholesteryl ester transfer protein (CETP) in lipid metabolism is still unclear. Furthermore, the relationship of CETP and atherosclerosis (AS) has been controversial. As pigs are a good model for both lipid and AS research, we investigated the lipid metabolism of human CETP (hCETP) transgenic pigs and explored the mechanism of CETP in lipid modulation. METHODS: Plasmids expressing the hCETP gene were designed, successfully constructed, and transfected into porcine fetal fibroblasts by liposomes...
September 11, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28881081/a-genetically-inducible-porcine-model-of-intestinal-cancer
#9
Morten Møbjerg Callesen, Sigrid Salling Árnadóttir, Iben Lyskjaer, Mai-Britt Worm Ørntoft, Søren Høyer, Frederik Dagnaes-Hansen, Ying Liu, Rong Li, Henrik Callesen, Mads Heilskov Rasmussen, Martin Fogtmann Berthelsen, Martin Kristian Thomsen, Pawel Jan Schweiger, Kim Bak Jensen, Søren Laurberg, Torben Falck Ørntoft, Jannik Ejnar Elverløv-Jakobsen, Claus Lindbjerg Andersen
Transgenic porcine cancer models bring novel possibilities for research. Their physical similarities with humans enable the use of surgical procedures and treatment approaches used for patients, which facilitates clinical translation. Here, we aimed to develop an inducible oncopig model of intestinal cancer. Transgenic (TG) minipigs were generated using somatic cell nuclear transfer by hand-made cloning. The pigs encode two TG cassettes: 1) An Flp-recombinase inducible oncogene cassette containing KRAS-G12D, cMYC, SV40LT - which inhibits p53 - and pRB...
September 7, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28864025/birth-weight-umbilical-and-placental-traits-in-relation-to-neonatal-loss-in-cloned-pigs
#10
Zheng Ao, Dewu Liu, Chengfa Zhao, Zhimin Yue, Junsong Shi, Rong Zhou, Gengyuan Cai, Enqin Zheng, Zicong Li, Zhenfang Wu
Cloned piglets generated through somatic cell nuclear transfer (SCNT) have a high rate of neonatal death. Postnatal loss is associated with low birth weight, umbilical status and placental parameters in fertilisation-derived piglets. To investigate whether or not this relationship also exists in cloned piglets, birth weight, umbilical status, placental parameters, placental morphology and gene expression pattern were compared among four piglet groups, namely, SCNT-derived male piglets that died within 4 days (SCNT-DW4), SCNT-derived male piglets that survived over 4 days (SCNT-SO4), artificial insemination (AI)-generated male piglets that died within 4 days (AI-DW4) and AI-generated male piglets that survived over 4 days (AI-SO4)...
September 2017: Placenta
https://www.readbyqxmd.com/read/28860480/discovery-of-human-like-l-asparaginases-with-potential-clinical-use-by-directed-evolution
#11
Coraline Rigouin, Hien Anh Nguyen, Amanda M Schalk, Arnon Lavie
L-asparaginase is a chemotherapy drug used to treat acute lymphoblastic leukemia (ALL). The main prerequisite for clinical efficacy of L-asparaginases is micromolar KM for asparagine to allow for complete depletion of this amino acid in the blood. Since currently approved L-asparaginases are of bacterial origin, immunogenicity is a challenge, which would be mitigated by a human enzyme. However, all human L-asparaginases have millimolar KM for asparagine. We recently identified the low KM guinea pig L-asparaginase (gpASNase1)...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28858207/x-chromosome-evolution-in-cetartiodactyla
#12
Anastasia A Proskuryakova, Anastasia I Kulemzina, Polina L Perelman, Alexey I Makunin, Denis M Larkin, Marta Farré, Anna V Kukekova, Jennifer Lynn Johnson, Natalya A Lemskaya, Violetta R Beklemisheva, Melody E Roelke-Parker, June Bellizzi, Oliver A Ryder, Stephen J O'Brien, Alexander S Graphodatsky
The phenomenon of a remarkable conservation of the X chromosome in eutherian mammals has been first described by Susumu Ohno in 1964. A notable exception is the cetartiodactyl X chromosome, which varies widely in morphology and G-banding pattern between species. It is hypothesized that this sex chromosome has undergone multiple rearrangements that changed the centromere position and the order of syntenic segments over the last 80 million years of Cetartiodactyla speciation. To investigate its evolution we have selected 26 evolutionarily conserved bacterial artificial chromosome (BAC) clones from the cattle CHORI-240 library evenly distributed along the cattle X chromosome...
August 31, 2017: Genes
https://www.readbyqxmd.com/read/28853251/-expression-and-antiviral-activity-of-a-chimeric-porcinized-monoclonal-antibody-chq06-against-e2-protein-of-classical-swine-fever-virus
#13
Shucheng Chen, Huimin Sun, Su Li, Pinghuang Liu, Jifei Ma, Huaji Qiu
Classical swine fever (CSF), one of OIE-listed diseases, is a highly contagious and economically important disease of pigs. Classical swine fever virus (CSFV) is the causative agent of CSF. The capsid (C) protein and the glycoproteins Erns, E1 and E2, are structural components of the virus. E2 is the most immunogenic protein of the CSFV glycoproteins, inducing neutralizing antibodies that provide protection against lethal CSFV challenge. In a previous study, we developed a murine MAb HQ06 against the E2 protein of CSFV...
August 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28837849/isolation-and-molecular-cloning-of-hepatocyte-growth-factor-from-guinea-pig-ghgf-and-expression-of-truncated-variant-of-ghgf-with-improved-anti-fibrotic-activity-in-escherichia-coli
#14
Haifeng Liu, Xiaohua Wang, Ruijie Han, Kuiyang Zuo, Xiaohuan Yuan, Yuting Li, Jinglin Zhou, Lei Yan, Yanhui Chu
Hepatocyte growth factor (HGF) is an attractive target for anti-fibrotic therapy because it attenuates excessive transforming growth factor-β1 (TGF-β1) which plays an important role in hepatic fibrosis. In the study, we reported on the isolation and molecular cloning of the open reading frame (ORF) of guinea pig HGF (gHGF), encoding a protein of 729 amino acids, with an apple-like (hairpin) domain, four kringle domains and a trypsin-like serine protease domain. Moreover, the truncated variant of gHGF (a double mutant of N-terminal hairpin and first kringle domains of gHGF, K132E and G134E, gmNK1) protein fused with His6 tag, the molecular weight of which was about 20...
August 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28832658/effects-of-equol-on-multiple-k-channels-stably-expressed-in-hek-293-cells
#15
Xiu-Ling Deng, Yan Wang, Guo-Sheng Xiao
The present study investigated the effects of equol on cardiovascular K+ channel currents. The cardiovascular K+ channel currents were determined in HEK 293 cells stably expressing cloned differential cardiovascular K+ channels with conventional whole-cell patch voltage-clamp technique. We found that equol inhibited hKv1.5 (IC50: 15.3 μM), hKv4.3 (IC50: 29.2 μM and 11.9 μM for hKv4.3 peak current and charge area, respectively), IKs (IC50: 24.7 μM) and IhERG (IC50: 31.6 and 56.5 μM for IhERG.tail and IhERG...
2017: PloS One
https://www.readbyqxmd.com/read/28830563/orf1a-of-highly-pathogenic-prrs-attenuated-vaccine-virus-plays-a-key-role-in-neutralizing-antibody-induction-in-piglets-and-virus-neutralization-in-vitro
#16
Chaoliang Leng, Wuchao Zhang, Hongliang Zhang, Yunchao Kan, Lunguang Yao, Hongyue Zhai, Mingliang Li, Zhen Li, Chunxiao Liu, Tongqing An, Jinmei Peng, Qian Wang, Yumin Leng, Xuehui Cai, Zhijun Tian, Guangzhi Tong
BACKGROUND: Currently, porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important viral pathogens in swine in most countries, especially China. Two PRRSV attenuated live vaccine strains (HuN4-F112 and CH-1R) are currently widely used in China. Our previous study showed that HuN4-F112, but not CH-1R, induced high anti-nucleocapsid (N) antibody and neutralizing antibody (NA) titers. Additionally, sera from HuN4-F112 inoculated pigs induced low cross neutralization of CH-1R...
August 22, 2017: Virology Journal
https://www.readbyqxmd.com/read/28821716/transcriptome-analysis-reveals-long-intergenic-non-coding-rnas-involved-in-skeletal-muscle-growth-and-development-in-pig
#17
Cheng Zou, Jingxuan Li, Wenzhe Luo, Long Li, An Hu, Yuhua Fu, Ye Hou, Changchun Li
Long intergenic non-coding RNAs (lincRNAs) play essential roles in numerous biological processes and are widely studied. The skeletal muscle is an important tissue that plays an essential role in individual movement ability. However, lincRNAs in pig skeletal muscles are largely undiscovered and their biological functions remain elusive. In this study, we assembled transcriptomes using RNA-seq data published in previous studies of our laboratory group and identified 323 lincRNAs in porcine leg muscle. We found that these lincRNAs have shorter transcript length, fewer exons and lower expression level than protein-coding genes...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807497/characterisation-of-the-porcine-cytokines-which-activate-the-cd131%C3%AE-c-common-sub-unit-for-potential-immune-augmentation
#18
G Stephenson, K R Morris, T E O'Neil, M P Bruce, A D G Strom, A G D Bean
Early acting cytokines and growth factors such as those of the CD131 βc subunit, may offer an alternative method to the current use of antibiotics and chemicals such as anthelmintics in maintaining Porcine (Po) health. Thus far, the recombinant Po (rPo) Granulocyte-macrophage colony-stimulating factor (GM-CSF), rPo interleukin-3 (IL-3) and rPo interleukin-5 (IL-5) proteins have been identified and cloned and the biological activity of each cytokine has been confirmed in vitro, however, in vivo immune system regulation and hematopoietic stem cell (HSC) augmentation are regulated by numerous cytokines and cellular signals within the bone marrow (BM) niche...
August 11, 2017: Cytokine
https://www.readbyqxmd.com/read/28807097/genotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-pigs-and-retail-foods-in-china
#19
Wei Wang, Feng Liu, Zulqarnain Baloch, Cun Shan Zhang, Ke Ma, Zi Xin Peng, Shao Fei Yan, Yu Jie Hu, Xin Gan, Yin Ping Dong, Yao Bai, Feng Qin Li, Xiao Mein Yan, Ai Guo Ma, Jin Xu
OBJECTIVE: To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. METHODS: Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28768854/presence-of-a-shared-5-leader-sequence-in-ancestral-human-and-mammalian-retroviruses-and-its-transduction-into-feline-leukemia-virus
#20
Junna Kawasaki, Maki Kawamura, Yoshiharu Ohsato, Jumpei Ito, Kazuo Nishigaki
Recombination events induce significant genetic changes, and this process can result in virus genetic diversity or in the generation of novel pathogenicity. We discovered a new recombinant feline leukemia virus (FeLV) gag gene harboring an unrelated insertion, termed the X region, which was derived from Felis catus endogenous gammaretrovirus 4 (FcERV-gamma4). The identified FcERV-gamma4 proviruses have lost their coding capabilities, but some can express their viral RNA in feline tissues. Although the X-region-carrying recombinant FeLVs appeared to be replication-defective viruses, they were detected in 6...
October 15, 2017: Journal of Virology
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