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cloning pig

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https://www.readbyqxmd.com/read/28622855/b-cell-cross-epitope-of-propionibacterium-acnes-and-actinobacillus-pleuropneumonia-selected-by-phage-display-library-can-efficiently-protect-from-actinobacillus-pleuropneumonia-infection
#1
Jianfang Liu, Qiuyue Ma, Feng Yang, Rining Zhu, Jingmin Gu, Changjiang Sun, Xin Feng, Chongtao Du, Paul R Langford, Wenyu Han, Junling Yang, Liancheng Lei
Contagious porcine pleuropneumonia (CPP), caused by Actinobacillus pleuropneumoniae (APP), is a highly transmissible and fatal respiratory illness that causes tremendous economic losses for the pig breeding industry worldwide. Propionibacterium acnes (PA) has a strong cross-reaction with anti-APP1 and anti-APP5 serum and can efficiently prevent APP infection, which was fortuitously found in researching the differential gene between the different APP serotypes. There seems to be some natural cross-protection between PA and APP...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28614361/molecular-cloning-characterization-and-expression-analysis-of-frizzled-6-in-the-small-intestine-of-pigs-sus-scrofa
#2
Lijun Zou, Xiaocheng Wang, Liping Jiang, Shengping Wang, Xia Xiong, Huansheng Yang, Wei Gao, Min Gong, Chien-An A Hu, Yulong Yin
Frizzled 6 (FZD6) encodes an integral membrane protein that functions in multiple signal transduction pathways, for example, as a receptor in Wnt/planar cell polarity (PCP) signaling pathway for polarized cell migration and organ morphogenesis. Mutations in FZD6 have been identified in a variety of tumors. In this study, the full-length cDNA of Sus scrofa FZD6 (Sfz6) was cloned and characterized. Nucleotide sequence analysis demonstrates that the Sfz6 gene encodes the 712 amino-acid (aa) protein with seven transmembrane domain...
2017: PloS One
https://www.readbyqxmd.com/read/28609368/derivation-and-characterization-of-a-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line
#3
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
OBJECTIVES: The aim of this study was to identify an epithelial cell line isolated from the spontaneous differentiation of totipotent pig epiblast cells. METHODS: PICM-31 and its colony-cloned derivative cell line, PICM-31A, were established from the culture and differentiation of an epiblast mass isolated from an 8-day-old pig blastocyst. The cell lines were analyzed by transmission electron microscopy, marker gene expression, and mass spectroscopy-based proteomics...
July 2017: Pancreas
https://www.readbyqxmd.com/read/28597956/a-post-gwas-confirming-gpat3-gene-associated-with-pig-growth-and-a-significant-snp-influencing-its-promoter-activity
#4
Kejun Wang, Yufang Liu, Qiao Xu, Chengkun Liu, Jve Wang, Cui Ding, Meiying Fang
Our previous genome-wide association study revealed that a SNP, ASGA0040372, located upstream of glycerol-3-phosphate acyltransferase 3 (GPAT3), is related to growth traits in Duroc pigs. In this study, we aimed to extensively investigate the genetic variation of GPAT3 and to further verify the contribution of ASGA0040372 to growth traits and its effect on promoter activity. Our data, obtained based on gene cloning and rapid amplification of cDNA ends experiments, showed that there are 14 exons and three alternative splicing variants...
June 9, 2017: Animal Genetics
https://www.readbyqxmd.com/read/28583605/in%C3%A2-vitro-development-and-cytological-quality-of-inter-species-porcine%C3%A2-bovine-cloned-embryos-are-affected-by-trichostatin-a-dependent-epigenomic-modulation-of-adult-mesenchymal-stem-cells
#5
J Opiela, M Samiec, J Romanek
Artificial epigenomic modulation of in vitro cultured mesenchymal stem cells (MSCs) by applying a non-selective HDAC inhibitor, termed TSA, can facilitate more epigenetic reprogramming of transcriptional activity of the somatic cell-descended nuclear genome in NT pig embryos. The results of the present investigation showed that TSA-dependent epigenomic modulation of nuclear donor MSCs highly affects both the in vitro developmental capability and the cytological quality of inter-species (porcine→bovine) cloned embryos...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28568977/reproductive-performance-and-expression-of-imprinted-genes-in-somatic-cell-cloned-boars
#6
Tatsuo Kawarasaki, Satoko Enya, Masayoshi Otake, Masatoshi Shibata, Satoshi Mikawa
To assess the performance of boars derived by somatic cell cloning, we analyzed various aspects of their reproductive characteristics and the expression of two imprinted genes. Cloned boars (cloned Duroc × Jinhua) were analyzed for birth weight, growth rate, age at first ejaculation, semen characteristics and fertility, in comparison with naturally bred control boars of the same strain. The expression of imprinted genes was analyzed using the microsatellite marker SWC9 for the paternally expressed gene insulin-like growth factor -2 (IGF2) and with single nucleotide polymorphisms (SNPs) for the gene maternally expressed 3 (MEG3)...
June 1, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28553699/generation-of-cmahko-gtko-shtnfri-fc-ho-1-quadruple-gene-modified-pigs
#7
Geon A Kim, Eun Mi Lee, Jun-Xue Jin, Sanghoon Lee, Anukul Taweechaipaisankul, Jong Ik Hwang, Zahid Alam, Curie Ahn, Byeong Chun Lee
As an alternative source of organs for transplantation into humans, attention has been directed to pigs due to their similarities in biological features and organ size. However, severe immune rejection has prevented successful xenotransplantation using pig organs and tissues. To overcome immune rejection, recently developed genetic engineering systems such as TALEN coupled with somatic cell nuclear transfer (SCNT) to make embryos could be used to produce pigs compatible with xenotransplantation. We used the TALEN system to target the non-Gal antigen cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) gene in pigs that is naturally deleted in humans...
May 28, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28552396/the-effect-of-histone-acetyl-transferase-inhibitor-trichostatin-a-on-porcine-mesenchymal-stem-cell-transcriptome
#8
Artur Gurgul, Jolanta Opiela, Klaudia Pawlina, Tomasz Szmatoła, Michał Bochenek, Monika Bugno-Poniewierska
The use of histone acetyl-transferase inhibitors such as trichostatin A (TSA) for epigenetic transformation of mesenchymal stem cells (MSCs), whose nuclei will be transferred into enucleated oocytes, is a novel approach in research involving somatic cell cloning of pigs and other mammalian species. Although the effectiveness of TSA in cloning applications was confirmed, processes and mechanisms underlying achieved effects are not yet fully understood, especially for pig MSCs. To contribute to this knowledge, in this study we performed a comprehensive transcriptome analysis using high-throughput sequencing of pig bone-marrow derived MSCs, both treated and untreated with TSA, and evaluated the effect of TSA administration on their transcription profile after 24 h of in vitro culture...
May 25, 2017: Biochimie
https://www.readbyqxmd.com/read/28534071/cloning-of-helicobacter-suis-cholesterol-%C3%AE-glucosyltransferase-and-production-of-an-antibody-capable-of-detecting-it-in-formalin-fixed-paraffin-embedded-gastric-tissue-sections
#9
Masatomo Kawakubo, Kazuki Horiuchi, Hitomi Komura, Yoshiko Sato, Masayoshi Kato, Meguru Ikeyama, Mana Fukushima, Shigenori Yamada, Satoshi Ishizone, Takehisa Matsumoto, Hiroyoshi Ota, Junji Sagara, Jun Nakayama
Helicobacter suis (H. suis), formerly called Helicobacter heilmannii type 1 (H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and rodents, the latter giving rise to zoonotic infection. Here, we generated a H. suis-specific antibody useful for immunohistochemistry with formalin-fixed, paraffin-embedded tissue sections. To do so, we began by cloning the gene encoding H. suis cholesterol α-glucosyltransferase (αCgT)...
May 22, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28531364/analysis-of-the-internal-transcribed-spacer-region-of-ascaris-suum-and-ascaris-lumbricoides-derived-from-free-range-tibetan-pigs
#10
Kun Li, Houqiang Luo, Hui Zhang, Khalid Mehmood, Muhammad Shahzad, Lihong Zhang, Jiakui Li
Ascaris suum (A. suum) is the most commonly occurring worldwide internal parasite of pigs; however, little is known about this organism in Tibetan pigs in China. A study was carried to isolate and identify the characteristics of internal transcribed spacer region (ITS) gene of A. suum derived from Tibetan pigs. Adult nematodes were collected from Tibetan pigs in 2015-2016. Total genomic DNA of the extracted parasites was performed and a fragment of the ITS of mitochondrial (mt) gene was amplified. The amplicons were cloned into PGEM®-T Easy Vector (Promega, WI) and the positive clones were sequenced by ABI 3730 × 1 sequencer...
May 21, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28525756/immunization-elicited-broadly-protective-antibody-reveals-ebolavirus-fusion-loop-as-a-site-of-vulnerability
#11
Xuelian Zhao, Katie A Howell, Shihua He, Jennifer M Brannan, Anna Z Wec, Edgar Davidson, Hannah L Turner, Chi-I Chiang, Lin Lei, J Maximilian Fels, Hong Vu, Sergey Shulenin, Ashley N Turonis, Ana I Kuehne, Guodong Liu, Mi Ta, Yimeng Wang, Christopher Sundling, Yongli Xiao, Jennifer S Spence, Benjamin J Doranz, Frederick W Holtsberg, Andrew B Ward, Kartik Chandran, John M Dye, Xiangguo Qiu, Yuxing Li, M Javad Aman
While neutralizing antibodies are highly effective against ebolavirus infections, current experimental ebolavirus vaccines primarily elicit species-specific antibody responses. Here, we describe an immunization-elicited macaque antibody (CA45) that clamps the internal fusion loop with the N terminus of the ebolavirus glycoproteins (GPs) and potently neutralizes Ebola, Sudan, Bundibugyo, and Reston viruses. CA45, alone or in combination with an antibody that blocks receptor binding, provided full protection against all pathogenic ebolaviruses in mice, guinea pigs, and ferrets...
May 18, 2017: Cell
https://www.readbyqxmd.com/read/28503569/postneonatal-mortality-and-liver-changes-in-cloned-pigs-associated-with-human-tumor-necrosis-factor-receptor-i-fc-and-human-heme-oxygenase-1-overexpression
#12
Geon A Kim, Jun-Xue Jin, Sanghoon Lee, Anukul Taweechaipaisankul, Hyun Ju Oh, Joing-Ik Hwang, Curie Ahn, Islam M Saadeldin, Byeong Chun Lee
Soluble human tumor necrosis factor (shTNFRI-Fc) and human heme oxygenase 1 (hHO-1) are key regulators for protection against oxidative and inflammatory injury for xenotransplantation. Somatic cells with more than 10 copy numbers of shTNFRI-Fc and hHO-1 were employed in somatic cell nuclear transfer to generate cloned pigs, thereby resulting in seven cloned piglets. However, produced piglets were all dead within 24 hours after birth. Obviously, postnatal death with liver apoptosis was reported in the higher copy number of shTNFRI-Fc and hHO-1 piglets...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28500340/both-haemagglutinin-specific-antibody-and-t-cell-responses-induced-by-a-chimpanzee-adenoviral-vaccine-confer-protection-against-influenza-h7n9-viral-challenge
#13
Xiang Wang, Weihui Fu, Songhua Yuan, Xi Yang, Yufeng Song, Lulu Liu, Yudan Chi, Tao Cheng, Man Xing, Yan Zhang, Chao Zhang, Yong Yang, Caihong Zhu, Xiaoyan Zhang, Sidong Xiong, Jianqing Xu, Dongming Zhou
Since 2013, the outbreak or sporadic infection of a new reassortant H7N9 influenza virus in China has resulted in hundreds of deaths and thousands of illnesses. An H7N9 vaccine is urgently needed, as a licensed human vaccine against H7N9 influenza is currently not available. Here, we developed a recombinant adenovirus-based vaccine, AdC68-H7HA, by cloning the H7N9 haemagglutinin (HA) gene into the chimpanzee adenoviral vector AdC68. The efficacy of AdC68-H7HA was evaluated in mice as well as guinea pigs. For comparison, an H7N9 DNA vaccine based on HA was also generated and tested in mice and guinea pigs...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28490591/deletion-of-a-197-amino-acid-region-in-the-n-terminal-domain-of-spike-protein-attenuates-porcine-epidemic-diarrhea-virus-in-piglets
#14
Yixuan Hou, Chun-Ming Lin, Masaru Yokoyama, Boyd L Yount, Douglas Marthaler, Arianna L Douglas, Shristi Ghimire, Yibin Qin, Ralph S Baric, Linda J Saif, Qiuhong Wang
We previously isolated a porcine epidemic diarrhea virus (PEDV) strain PC177, by blind serial passaging the intestinal contents of a diarrheic piglet in Vero cell culture. Compared with a US highly virulent PEDV strain PC21A, the tissue culture-adapted PC177 (TC-PC177) contains a 197 aa-deletion in the N-terminal domain of the spike protein. We orally inoculated neonatal, conventional suckling piglets with TC-PC177 or PC21A to compare the pathogenicity. Within 7 days post-inoculation, TC-PC177 caused mild diarrhea and lower fecal viral RNA shedding, with no mortality, whereas PC21A caused severe clinical signs and 55% mortality...
May 10, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28487309/molecular-cloning-and-characterization-of-marmoset-aldehyde-oxidase
#15
Shotaro Uehara, Yasuhiro Uno, Eriko Okamoto, Takashi Inoue, Erika Sasaki, Hiroshi Yamazaki
Common marmosets (Callithrix jacchus), New world monkeys, are a promising primate model for preclinical drug metabolism studies due to the similarities of cytochrome P450 (P450) enzyme function to those of humans. Despite an increasing number of drug candidates catalyzed by non-P450 enzymes, drug metabolizing enzymes other than P450s have been hardly identified or characterized in marmosets. In this study, we identified aldehyde oxidase (AOX) 1 gene by marmoset genome analysis. AOX1 cDNA was cloned from marmoset livers by reverse transcription-polymerase chain reaction...
May 9, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28448603/evaluation-of-immunogenicity-and-protective-efficacy-of-recombinant-outer-membrane-proteins-of-haemophilus-parasuis-serovar-5-in-a-murine-model
#16
Miao Li, Ru-Jian Cai, Shuai Song, Zhi-Yong Jiang, Yan Li, Hong-Chao Gou, Pin-Pin Chu, Chun-Ling Li, Hua-Ji Qiu
Glässer's disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28442343/pig-has-no-uncoupling-protein-1
#17
Lianjie Hou, Jia Shi, Lingbo Cao, Guli Xu, Chingyuan Hu, Chong Wang
Brown adipose tissue (BAT) is critical for mammal's survival in the cold environment. Uncoupling protein 1 (UCP1) is responsible for the non-shivering thermogenesis in the BAT. Pig is important economically as a meat-producing livestock. However, whether BAT or more precisely UCP1 protein exists in pig remains a controversy. The objective of this study was to ascertain whether pig has UCP1 protein. In this study, we used rapid amplification of cDNA ends (RACE) technique to obtain the UCP1 mRNA 3' end sequence, confirmed only exons 1 and 2 of the UCP1 gene are transcribed in the pig...
June 10, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28434792/cloning-and-expression-patterns-of-neuromedin-u-and-its-receptors-in-pigs
#18
Xiang Li, Mingfu Niu, Juan Su, Zhiyu Ma, Mengmeng Jin, Wenna Qiao, Ying Zhang, Yanyan Feng, Na An, Yuanlong Hou, Sheng Yang, Suo Chuan, Zhihai Lei
Neuromedin U (NMU) is a highly conserved neuropeptide that performs a variety of physiological functions in animals via neuromedin U receptor-1 (NMUR1) and neuromedin U receptor-2 (NMUR2). In this study, we cloned the pig NMU, NMUR1 and NMUR2 genes. Bioinformatics analysis demonstrated that the pig NMU cDNA encoded the amino acids Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2 at the C-terminus and that the NMU receptors, which are G-protein-coupled receptors (GPCRs), contained the seven transmembrane domains typical of GPCRs...
April 6, 2017: Neuropeptides
https://www.readbyqxmd.com/read/28421046/horses-in-denmark-are-a-reservoir-of-diverse-clones-of-methicillin-resistant-and-susceptible-staphylococcus-aureus
#19
Md Zohorul Islam, Carmen Espinosa-Gongora, Peter Damborg, Raphael N Sieber, Rikke Munk, Louise Husted, Arshnee Moodley, Robert Skov, Jesper Larsen, Luca Guardabassi
Denmark is a country with high prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 in pigs. Even though pig farming is regarded as the main source of human infection or colonization with MRSA CC398, 10-15% of the human cases appear not to be linked to pigs. Following the recent reports of MRSA CC398 in horses in other European countries and the lack of knowledge on S. aureus carriage in this animal species, we carried out a study to investigate whether horses constitute a reservoir of MRSA CC398 in Denmark, and to gain knowledge on the frequency and genetic diversity of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28389048/postnatal-developmental-of-neuromedin-s-and-its-receptor-in-the-male-xiaomeishan-pig-reproductive-axis
#20
Zhiyu Ma, Yangyang Zhao, Yuan Yao, Zhihai Lei, Mengmeng Jin, Xiang Li, Cuicui Jia, Zheng Zhang, Xiaoliang Li, Juan Su
Neuromedin S (NMS) has been identified as an endogenous ligand for FM-3/GPR66 and FM-4/TGR-1 which are NMU receptors NMUR1 and NMUR2, respectively. The NMS molecule is present in some peripheral tissues and the central nervous system (CNS), and it had been documented that NMS has fundamental and important roles in multiple physiological functions and processes such as circadian rhythm, energy balance, feeding behavior, stress responses and reproduction. The possible role of NMS in sexual development postnatally, however, is still obscure...
April 2, 2017: Animal Reproduction Science
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