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https://www.readbyqxmd.com/read/28448603/evaluation-of-immunogenicity-and-protective-efficacy-of-recombinant-outer-membrane-proteins-of-haemophilus-parasuis-serovar-5-in-a-murine-model
#1
Miao Li, Ru-Jian Cai, Shuai Song, Zhi-Yong Jiang, Yan Li, Hong-Chao Gou, Pin-Pin Chu, Chun-Ling Li, Hua-Ji Qiu
Glässer's disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28442343/pig-has-no-uncoupling-protein-1
#2
Lianjie Hou, Jia Shi, Lingbo Cao, Guli Xu, Chingyuan Hu, Chong Wang
Brown adipose tissue (BAT) is critical for mammal's survival in the cold environment. Uncoupling protein 1 (UCP1) is responsible for the non-shivering thermogenesis in the BAT. Pig is important economically as a meat-producing livestock. However, whether BAT or more precisely UCP1 protein exists in pig remains a controversy. The objective of this study was to ascertain whether pig has UCP1 protein. In this study, we used rapid amplification of cDNA ends (RACE) technique to obtain the UCP1 mRNA 3' end sequence, confirmed only exons 1 and 2 of the UCP1 gene are transcribed in the pig...
April 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28434792/cloning-and-expression-patterns-of-neuromedin-u-and-its-receptors-in-pigs
#3
Xiang Li, Mingfu Niu, Juan Su, Zhiyu Ma, Mengmeng Jin, Wenna Qiao, Ying Zhang, Yanyan Feng, Na An, Yuanlong Hou, Sheng Yang, Suo Chuan, Zhihai Lei
Neuromedin U (NMU) is a highly conserved neuropeptide that performs a variety of physiological functions in animals via neuromedin U receptor-1 (NMUR1) and neuromedin U receptor-2 (NMUR2). In this study, we cloned the pig NMU, NMUR1 and NMUR2 genes. Bioinformatics analysis demonstrated that the pig NMU cDNA encoded the amino acids Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2 at the C-terminus and that the NMU receptors, which are G-protein-coupled receptors (GPCRs), contained the seven transmembrane domains typical of GPCRs...
April 6, 2017: Neuropeptides
https://www.readbyqxmd.com/read/28421046/horses-in-denmark-are-a-reservoir-of-diverse-clones-of-methicillin-resistant-and-susceptible-staphylococcus-aureus
#4
Md Zohorul Islam, Carmen Espinosa-Gongora, Peter Damborg, Raphael N Sieber, Rikke Munk, Louise Husted, Arshnee Moodley, Robert Skov, Jesper Larsen, Luca Guardabassi
Denmark is a country with high prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 in pigs. Even though pig farming is regarded as the main source of human infection or colonization with MRSA CC398, 10-15% of the human cases appear not to be linked to pigs. Following the recent reports of MRSA CC398 in horses in other European countries and the lack of knowledge on S. aureus carriage in this animal species, we carried out a study to investigate whether horses constitute a reservoir of MRSA CC398 in Denmark, and to gain knowledge on the frequency and genetic diversity of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28389048/postnatal-developmental-of-neuromedin-s-and-its-receptor-in-the-male-xiaomeishan-pig-reproductive-axis
#5
Zhiyu Ma, Yangyang Zhao, Yuan Yao, Zhihai Lei, Mengmeng Jin, Xiang Li, Cuicui Jia, Zheng Zhang, Xiaoliang Li, Juan Su
Neuromedin S (NMS) has been identified as an endogenous ligand for FM-3/GPR66 and FM-4/TGR-1 which are NMU receptors NMUR1 and NMUR2, respectively. The NMS molecule is present in some peripheral tissues and the central nervous system (CNS), and it had been documented that NMS has fundamental and important roles in multiple physiological functions and processes such as circadian rhythm, energy balance, feeding behavior, stress responses and reproduction. The possible role of NMS in sexual development postnatally, however, is still obscure...
April 2, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28387970/mutation-of-the-xist-gene-up-regulates-expression-of-x-linked-genes-but-decreases-the-developmental-rates-of-cloned-male-porcine-embryos
#6
Yang Yang, Dan Wu, Dewu Liu, Junsong Shi, Rong Zhou, Xiaoyan He, Jianping Quan, Gengyuan Cai, Enqin Zheng, Zhenfang Wu, Zicong Li
XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than tenfold. This study aimed to investigate if a similar manipulation would improve cloning efficiency in pigs. A male, porcine kidney cell line containing an EGFP insert in exon 1 of the XIST gene, resulting in a knockout allele (XIST-KO), was generated by homologous recombination using transcription activator-like effector nucleases (TALENs)...
April 7, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28380503/histone-deacetylase-inhibitor-m344-significantly-improves-nuclear-reprogramming-blastocyst-quality-and-in-vitro-developmental-capacity-of-cloned-pig-embryos
#7
L Jin, Q Guo, H-Y Zhu, X-X Xing, G-L Zhang, M-F Xuan, Q-R Luo, Z-B Luo, J-X Wang, H-M Choe, H-J Paek, X-J Yin, J-D Kang
M344 is a novel histone deacetylase inhibitor. There is no report on the effect of M344 treatment on the development of pig embryos after somatic cell nuclear transfer (SCNT). In the present study, we investigated the effect of M344 on the blastocyst formation rate in cloned embryos, acetylation level of histone H4 lysine 12 (AcH4K12), and the expression of pluripotency-related genes , , and . Our results indicated that treatment with 5 µ M344 for 6 h improved the development of porcine embryos, in comparison with the untreated group (25...
March 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28380421/dnmt1s-in-donor-cells-is-a-barrier-to-scnt-mediated-dna-methylation-reprogramming-in-pigs
#8
Xuexiong Song, Zhonghua Liu, Hongbin He, Jianyu Wang, Huatao Li, Jingyu Li, Fangzheng Li, Zhongling Jiang, Yanjun Huan
Low development of somatic cell nuclear transfer embryos could be due to the incomplete DNA methylation reprogramming, and Dnmt1s existing in donor cells may be one cause of this disrupted DNA methylation reprogramming. However, the reprogramming pattern of Dnmt1s and its effect on DNA methylation reprogramming in cloned embryos remain poorly understood. Here, we displayed that along with the significantly higher Dnmt1 expression at the zygotic gene activation stage of cloned embryos, genomic methylation level was markedly upregulated, and the arrested rate was significantly higher compared with their in vitro fertilization counterparts...
March 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28373351/key-role-of-capsular-polysaccharide-in-the-induction-of-systemic-infection-and-abortion-by-hypervirulent-campylobacter-jejuni
#9
Orhan Sahin, Samantha A Terhorst, Eric R Burrough, Zhangqi Shen, Zuowei Wu, Lei Dai, Yizhi Tang, Paul J Plummer, Ju Ji, Michael J Yaeger, Qijing Zhang
Campylobacter jejuni is a zoonotic pathogen, and a hypervirulent strain, named clone SA, has recently emerged as the predominant cause of ovine abortion in the United States. To induce abortion, orally ingested Campylobacter must translocate across the intestinal epithelium, spread systemically in the circulation, and reach the fetoplacental tissue. Bacterial factors involved in these steps are not well understood. C. jejuni is known to produce capsular polysaccharide (CPS), but the specific role CPS plays in systemic infection and particularly abortion in animals remains to be determined...
April 3, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28365311/clinical-impact-and-prevalence-of-mrsa-cc398-and-differences-between-mrsa-tet-r-and-mrsa-tet-s-in-an-area-of-spain-with-a-high-density-of-pig-farming-a-prospective-cohort-study
#10
E Reynaga, C Torres, M Garcia-Nuñez, M Navarro, A Vilamala, E Puigoriol, G E Lucchetti, M Sabrià
OBJETIVES: Tetracycline-resistance (Tet(R)) is a phenotypic marker of the livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) CC398 clone. The aim of this study was to analyze the prevalence of MRSA CC398 in patients in contact with healthcare facilities and differences between patients with MRSA-Tet(R) and MRSA tetracycline-susceptible (Tet(S)) strains. METHODS: Patients diagnosed with MRSA from January-2012 until December-2015 were divided into two groups, MRSA-Tet(R) and MRSA-Tet(S)...
March 29, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28360383/cloning-and-expression-analysis-of-zygote-arrest-1-zar1-in-new-zealand-white-rabbits
#11
Dan Wang, Shu-Yu Xie, Wei Zhang, Cai-Xia Sun, Tao Huang, An-Si Wang, Xue-Lei Han, Gui-Rong Sun, Ming Li
Zygote arrest 1 (Zar1) is an oocyte-specific maternal-effect gene. Previous studies indicate that Zar1 plays important role in early embryo development, but little is known about its function in rabbit. The objectives of this study were to clone the New Zealand white rabbit Zar1 gene and to investigate its expression in various organs in groups of animals with different reproductive traits.We obtained a 709-bp Zar1 cDNA fragment consisting of an 8-bp exon 1, 161-bp exon 2, 75-bp exon 3, 271-bp exon 4 and 194-bp 3'sequences...
March 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28358284/molecular-cloning-of-porcine-sun5-gene-and-association-between-a-snp-with-litter-size-trait
#12
Zhenhua Zhao, Yonggang Liu
SUN domain-containing protein 5 (SUN5) is an important reproduction related gene. In this study, we cloned the full-length coding sequence of porcine SUN5 gene through RT-PCR. Sequence analysis of this gene revealed that the pig SUN5 gene encodes a protein of 383 amino acids that has high homology with the SUN5 protein of eight species: wild Bactrian camel (95%), alpaca (95%), Yangtze River dolphin (94%), sperm whale (94%), sheep (93%), black flying fox (93%), goat (92%), and horse (91%). This gene is structured into 13 exons and 12 introns as revealed by computer-assisted analysis...
March 30, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28330290/successful-cloning-of-an-adult-breeding-boar-from-the-novel-chinese-guike-no-1-swine-specialized-strain
#13
Jun-Yu Nie, Xiang-Xing Zhu, Bing-Kun Xie, Su-Qun Nong, Qing-Yan Ma, Hui-Yan Xu, Xiao-Gan Yang, Yang-Qing Lu, Ke-Huan Lu, Yu-Ying Liao, Sheng-Sheng Lu
Somatic cloning, also known as somatic cell nuclear transfer (SCNT), is a promising technology which has been expected to rapidly extend the population of elaborately selected breeding boars with superior production performance. Chinese Guike No. 1 pig breed is a novel swine specialized strain incorporated with the pedigree background of Duroc and Chinese Luchuan pig breeds, thus inherits an excellent production performance. The present study was conducted to establish somatic cloning procedures of adult breeding boars from the Chinese Guike No...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28301551/development-of-the-full-length-cdna-clones-of-two-porcine-epidemic-diarrhea-disease-virus-isolates-with-different-virulence
#14
Jie Li, Zhonghui Jin, Yueyi Gao, Lei Zhou, Xinna Ge, Xin Guo, Jun Han, Hanchun Yang
The recently emerged highly virulent variants of porcine epidemic and diarrhea virus (PEDV) remain a huge threat to the worldwide swine industry. Here, we describe the development of a bacterial artificial chromosome (BAC) reverse genetics system for PEDV based on two recent Chinese field isolates, namely CHM2013 and BJ2011C. Phylogenetically, CHM2013 is closely related to the vaccine strain SM98 whereas the isolate BJ2011C belongs to the GIIb group, a cluster that contains many recent pandemic strains. The full-length cDNA clones of the two isolates were constructed into BAC under the control of CMV promoter...
2017: PloS One
https://www.readbyqxmd.com/read/28288770/experimental-toxoplasma-gondii-infections-in-pigs-humoral-immune-response-estimation-of-specific-igg-avidity-and-the-challenges-of-reproducing-vertical-transmission-in-sows
#15
Walter Basso, Felix Grimm, Maja Ruetten, Vitomir Djokic, Radu Blaga, Xaver Sidler, Peter Deplazes
Ten pregnant sows were experimentally inoculated per os with T. gondii in order to investigate vertical and galactogenic transmission of the parasite and the evolution and maturation of the specific IgG humoral response in the sows and piglets. Five seronegative sows received 10(4)T. gondii (CZ isolate clone H3) sporulated oocysts during late-pregnancy (Exp. 1), three sows received 10(4) oocysts during mid-pregnancy (Exp. 2) and three sows from Exp. 1 (and two seronegative sows) were re-inoculated with 10(5) oocysts during a further pregnancy (late-pregnancy) (Exp...
March 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28284626/rabbit-hepatitis-e-virus-is-an-opportunistic-pathogen-in-specific-pathogen-free-rabbits-with-the-capability-of-cross-species-transmission
#16
Baoyuan Liu, Yani Sun, Taofeng Du, Yiyang Chen, Xinjie Wang, Baicheng Huang, Huixia Li, Yuchen Nan, Shuqi Xiao, Gaiping Zhang, Julian A Hiscox, En-Min Zhou, Qin Zhao
Hepatitis E virus (HEV) has been detected in rabbits, a recently identified natural reservoir. In this study, anti-HEV antibodies and viral RNA were detected in rabbits sourced from a specific-pathogen-free (SPF) rabbit vendor in Shaanxi Province, China. BLAST results of partial HEV ORF2 genes cloned here indicated that two viral strains circulated in the rabbits. Sequence determination of the complete genome (7302bp) of one strain and a partial ORF1 gene (1537bp) of the other strain showed that they shared 90% identity with one another and 78%-94% identity with other known rabbit HEVs...
March 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28284602/a-novel-chimeric-protein-composed-of-recombinant-mycoplasma-hyopneumoniae-antigens-as-a-vaccine-candidate-evaluated-in-mice
#17
Natasha Rodrigues de Oliveira, Sérgio Jorge, Charles Klazer Gomes, Caroline Rizzi, Violetta Dias Pacce, Thais Farias Collares, Leonardo Garcia Monte, Odir Antônio Dellagostin
Enzootic Pneumonia (EP) is caused by the Mycoplasma hyopneumoniae pathogenic bacteria, and it represents a significant respiratory disease that is responsible for major economic losses within the pig industry throughout the world. The bacterins that are currently commercially available have been proven to offer only partial protection against M. hyopneumoniae, and the development of more efficient vaccines is required. Several recombinant antigens have been evaluated via different immunization strategies and have been found to be highly immunogenic...
March 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28255271/ssc-mir-7134-3p-regulates-fat-accumulation-in-castrated-male-pigs-by-targeting-mark4-gene
#18
Kejun Wang, Wenting Li, Ying Bai, Wanjie Yang, Yao Ling, Meiying Fang
Castration of male pigs is a common practice used to reduce boar taint in commercial pork production, but it also significantly results in fat accumulation in carcass. Our previous study revealed a miRNA gene, ssc-miR-7134-3p that was implicated in adipogenesis. However, the relationship between ssc-miR-7134-3p and fat deposition due to castration is unknown. In the present study, we observed that ssc-miR-7134-3p targets the coding sequence (CDS) region of MARK4 based on bioinformatics analysis and dual-luciferase assays...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28254450/diabetic-phenotype-of-transgenic-pigs-introduced-by-dominant-negative-mutant-hepatocyte-nuclear-factor-1%C3%AE
#19
Kazuhiro Umeyama, Masami Nakajima, Takashi Yokoo, Masaki Nagaya, Hiroshi Nagashima
AIM: The present study aimed to identify the characteristics of genetically modified pigs carrying a mutant human gene as a research model for diabetes and its complications. METHODS: We developed a transgenic cloned pig (founder, male) carrying a mutant gene, i.e., human HNF-1α (P291fsinsC), which is responsible for maturity-onset diabetes of the young type 3. Transgenic progeny obtained via the artificial insemination of wild type (WT) sows with the cryopreserved sperm derived from the founder pig was pathologically examined...
May 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/28252054/enhancement-of-porcine-intramuscular-fat-content-by-overexpression-of-the-cytosolic-form-of-phosphoenolpyruvate-carboxykinase-in-skeletal-muscle
#20
Zijian Ren, Ying Wang, Yuanyuan Ren, Zhengwei Zhang, Weiwang Gu, Zhaoting Wu, Lingyi Chen, Lisha Mou, Rongfeng Li, Haiyuan Yang, Yifan Dai
Intramuscular fat (IMF) content has been generally recognized as a desirable trait in pork meat because of its positive effect on eating quality. An effective approach to enhance IMF content in pork is the generation of transgenic pigs. In this study, we used somatic cell nuclear transfer (SCNT) to generate cloned pigs exhibiting ectopic expression of phosphoenolpyruvate carboxykinase (PEPCK-C) driven by an α-skeletal-actin gene promoter, which was specifically expressed in skeletal muscle. Using qRT-PCR and Western blot analysis, we demonstrated that PEPCK-C was functionally expressed and had a significant effect on total fatty acid content in the skeletal muscle of the transgenic pigs, while the n-6/n-3 polyunsaturated fatty acid (PUFA) ratio showed no difference between transgenic and control pigs...
March 2, 2017: Scientific Reports
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