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https://www.readbyqxmd.com/read/28821716/transcriptome-analysis-reveals-long-intergenic-non-coding-rnas-involved-in-skeletal-muscle-growth-and-development-in-pig
#1
Cheng Zou, Jingxuan Li, Wenzhe Luo, Long Li, An Hu, Yuhua Fu, Ye Hou, Changchun Li
Long intergenic non-coding RNAs (lincRNAs) play essential roles in numerous biological processes and are widely studied. The skeletal muscle is an important tissue that plays an essential role in individual movement ability. However, lincRNAs in pig skeletal muscles are largely undiscovered and their biological functions remain elusive. In this study, we assembled transcriptomes using RNA-seq data published in previous studies of our laboratory group and identified 323 lincRNAs in porcine leg muscle. We found that these lincRNAs have shorter transcript length, fewer exons and lower expression level than protein-coding genes...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807497/characterisation-of-the-porcine-cytokines-which-activate-the-cd131%C3%AE-c-common-sub-unit-for-potential-immune-augmentation
#2
G Stephenson, K R Morris, T E O'Neil, M P Bruce, A D G Strom, A G D Bean
Early acting cytokines and growth factors such as those of the CD131 βc subunit, may offer an alternative method to the current use of antibiotics and chemicals such as anthelmintics in maintaining Porcine (Po) health. Thus far, the recombinant Po (rPo) Granulocyte-macrophage colony-stimulating factor (GM-CSF), rPo interleukin-3 (IL-3) and rPo interleukin-5 (IL-5) proteins have been identified and cloned and the biological activity of each cytokine has been confirmed in vitro, however, in vivo immune system regulation and hematopoietic stem cell (HSC) augmentation are regulated by numerous cytokines and cellular signals within the bone marrow (BM) niche...
August 11, 2017: Cytokine
https://www.readbyqxmd.com/read/28807097/genotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-pigs-and-retail-foods-in-china
#3
Wei Wang, Feng Liu, Zulqarnain Baloch, Cun Shan Zhang, Ke Ma, Zi Xin Peng, Shao Fei Yan, Yu Jie Hu, Xin Gan, Yin Ping Dong, Yao Bai, Feng Qin Li, Xiao Mein Yan, Ai Guo Ma, Jin Xu
OBJECTIVE: To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. METHODS: Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28768854/presence-of-a-shared-5-leader-sequence-in-ancestral-human-and-mammalian-retroviruses-and-its-transduction-into-feline-leukemia-virus
#4
Junna Kawasaki, Maki Kawamura, Yoshiharu Ohsato, Jumpei Ito, Kazuo Nishigaki
Recombination events induce significant genetic changes, and this process can result in virus genetic diversity or in the generation of novel pathogenicity. We discovered a new recombinant feline leukemia virus (FeLV) gag gene harboring an unrelated insertion, termed the "X-region", which was derived from FcERV-gamma4, a feline endogenous gammaretrovirus. The identified FcERV-gamma4 proviruses have lost their coding capability, but some can express their viral RNA in feline tissues. Although the X-region-carrying recombinant FeLVs appeared to be replication-defective viruses, they were detected in 6...
August 2, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28758619/sus-scrofa-papillomavirus-2-genetic-characterization-of-a-novel-suid-papillomavirus-from-wild-boar-in-germany
#5
Ellen Kathrin Link, Marc Hoferer, Birte Strobel, Kerstin Rigbers, Martin Christoph Langenmayer, Gerd Sutter, Robert Fux
We identified a novel papillomavirus, Sus scrofa papillomavirus 2 (SsPV2), which is the first papillomavirus associated with papillomas in pigs. In skin alterations of a German wild boar, showing typical gross and histological appearance of papillomas, papillomavirus-like particles were demonstrated by electron microscopy. Degenerate papillomavirus-specific primers were used to amplify and sequence parts of the viral DNA. Subsequently, the complete genomic DNA was cloned and sequenced. The SsPV2 genome had a length of 8218 bp, encoded the early proteins E6, E7, E1 and E2, the late proteins L1 and L2 and contained an upstream regulatory region...
July 31, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28748463/detection-of-in-vivo-mutation-in-the-pig-a-gene-of-mouse-bone-marrow-erythroids
#6
Takafumi Kimoto, Daishiro Miura
Detection of in vivo mutation is important for evaluating the health risks associated with chemicals. The Pig-a in vivo gene mutation assay has been developed over the last decade for this purpose. Most approaches for the assay, however, measure cells with a Pig-a mutant phenotype in erythrocytes from the peripheral blood, with the mutations causing the phenotype being difficult to determine directly. This chapter describes a procedure for detecting mutations in the Pig-a gene of phenotypically mutant mouse bone marrow erythroids, the precursors of peripheral blood erythrocytes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28748462/in-vivo-rat-t-lymphocyte-pig-a-assay-detection-and-expansion-of-cells-deficient-in-the-gpi-anchored-cd48-surface-marker-for-analysis-of-mutation-in-the-endogenous-pig-a-gene
#7
Vasily N Dobrovolsky, Javier Revollo, Dayton M Petibone, Robert H Heflich
The Pig-a assay is being developed as an in vivo gene mutation assay for regulatory safety assessments. The assay is based on detecting mutation in the endogenous Pig-a gene of treated rats by using flow cytometry to measure changes in cell surface markers of peripheral blood cells. Here we present a methodology for demonstrating that phenotypically mutant rat T-cells identified by flow cytometry contain mutations in the Pig-a gene, an important step for validating the assay. In our approach, the mutant phenotype T-cells are sorted into individual wells of 96-well plates and expanded into clones...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28748351/pig-tissue-factor-pathway-inhibitor-%C3%AE-fusion-immunoglobulin-inhibits-pig-tissue-factor-activity-in-human-plasma-moderately-more-efficiently-than-the-human-counterpart
#8
Chang-Yong Choi, Yeon-Hui Kim, Joonbeom Bae, Suk Jun Lee, Hyun Kyung Kim, Chung-Gyu Park, Taehoon Chun
OBJECTIVE: To determine the efficacy of soluble pig tissue factor pathway inhibitor fusion immunoglobulin (TFPI-Ig) in blocking pig to human xenogeneic blood coagulation. RESULTS: To generate pig TFPI-Ig or human TFPI-Ig, expression vector containing cDNA encoding pig TFPIα or human TFPIα combined with human constant Ig heavy chain region was cloned and introduced into CHO cells. After purification of pig TFPI-Ig and human TFPI-Ig, the inhibition of each recombinant protein on pig tissue factor (TF)-mediated blood coagulation was examined in human plasma...
July 26, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28744884/shear-stress-induced-total-blood-trauma-in-multiple-species
#9
Chris H H Chan, Ina Laura Pieper, Christian R Robinson, Yasmin Friedmann, Venkateswarlu Kanamarlapudi, Catherine A Thornton
The common complications in heart failure patients with implanted ventricular assist devices (VADs) include hemolysis, thrombosis, and bleeding. These are linked to shear stress-induced trauma to erythrocytes, platelets, and von Willebrand factor (vWF). Novel device designs are being developed to reduce the blood trauma, which will need to undergo in vitro and in vivo preclinical testing in large animal models such as cattle, sheep, and pig. To fully understand the impact of device design and enable translation of preclinical results, it is important to identify any potential species-specific differences in the VAD-associated common complications...
July 26, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28712958/enhancing-oxidative-stress-resistance-in-bifidobacterium-thermophilum-using-a-novel-overexpression-vector-and-transformation-protocol
#10
Marc J A Stevens, Adriano Venturini, Christophe Lacroix, Leo Meile
Bifidobacterium thermophilum is encountered in the GI-tract of pigs and infants. Here we provide a transformation protocol for B. thermophilum and a novel expression vector for this species. The protocol resulted in transformation rates of 1×10(3) transformed cells per μg DNA. Transformation was shown to be dependent on the presence of fructo-oligosaccharides during growth, polyethylene glycol in the electroporation buffer, and on methylation of the vector. The Escherichia coli - B. thermophilum shuttle vector pLFB1012 for heterologous gene expression was constructed harbouring the glyceraldehyde 3-phosphate dehydrogenase promoter from Bifidobacterium longum (Pgap)...
July 14, 2017: Plasmid
https://www.readbyqxmd.com/read/28703288/a-comparative-proteomic-analysis-of-blood-serum-for-developmental-stages-in-pigs
#11
K Srikanth, E Lee, A Kwon, J Shin, H Chung
This study aimed to differentiate genes at developmental stages of pigs from 0 to 150 days of age, to build up a protein database and to find candidate genetic markers for growth traits. The analysis of two-dimensional electrophoresis and matrix-assisted laser-desorption/ionization mass spectrometry separated 252 protein segments. After successfully blasting the peptide sequences, the analysis confirmed 37 differentially expressed proteins that increased from birth to 150 days of age (type A), whereas the type B proteins presented the inverse pattern...
July 12, 2017: Animal Genetics
https://www.readbyqxmd.com/read/28699875/the-middle-half-genome-of-interferon-inducing-porcine-reproductive-and-respiratory-syndrome-virus-strain-a2mc2-is-essential-for-interferon-induction
#12
Zexu Ma, Ying Yu, Yueqiang Xiao, Tanja Opriessnig, Rong Wang, Liping Yang, Yuchen Nan, Siba K Samal, Patrick G Halbur, Yan-Jin Zhang
Porcine reproductive and respiratory syndrome virus (PRRSV) is known to antagonize the innate immune response. An atypical PRRSV strain A2MC2 is capable of inducing synthesis of type I interferons (IFNs) in cultured cells. Here, we show that the middle half of the A2MC2 genome is needed for triggering the IFN synthesis. First, a cDNA infectious clone of this atypical strain was constructed as a DNA-launched version. Virus recovery was achieved from the infectious clone and the recovered virus, rA2MC2, was characterized...
July 13, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28693296/mineralized-deposits-in-the-uterus-of-a-pig-without-pregnancy-loss
#13
Geon A Kim, Jun-Xue Jin, Anukul Taweechaipaisankul, Sanghoon Lee, Byung Il Yoon, Jongki Cho, Byeong Chun Lee
We reported a case of uterine calcification in the uterus of a pig without pregnancy loss. The recipient underwent cloned embryo transfer and the Cesarean section for safe delivery of cloned piglets. During the Cesarean section, 4 white, starlike, 2 x 2 x 2 cm, calcified structures were found within the endometrial cavity. Despite dystrophic calcification around placenta, healthy cloned piglets were produced successfully. To our knowledge, this is the first reported case of dystrophic calcification occurring within the uterus in a pregnant pig...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28686540/replicon-particle-expressing-the-e2-glycoprotein-of-bovine-viral-diarrhea-virus-immunization-and-evaluation-of-antibody-response
#14
Mohammad M Hossain, Raymond R Rowland
The objective of this study was to develop a new antigen delivery system using an alphavirus replicon particle (RP) to induce humoral antibody responses against bovine viral diarrhea virus (BVDV) recombinant antigen produced from envelope glycoprotein E2. An alphavirus RP expressing the E2 glycoprotein of BVDV was used for immunization of pigs. A fluorescent microsphere immunoassay (FMIA) has been applied to detect BVDV E2 antigen-specific antibody isotype in pig immunized with alphavirus RP. Full-length BVDV E2 (aa 1-375) was cleaved into several pieces, eight E2 DNA fragments, including full-length DNA, were cloned into expression vector pHUE, and the recombinant proteins expressed in BL-21 (DE3) Escherichia coli...
July 7, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28674231/molecular-characterization-and-expression-of-the-gdf9-gene-in-new-zealand-white-rabbits
#15
Caixia Sun, Shuyu Xie, Tao Huang, Wei Zhang, Ansi Wang, Dan Wang, Ming Li, Guirong Sun
Growth differentiation factor 9 (GDF9) has been shown to be involved in regulating follicular development and reproduction in many mammalian species. However, related information about the effect of the GDF9 gene on reproductive traits of New Zealand white rabbits was rarely reported. In this study, rabbits were distributed into two groups (poor and prolific offspring productions) and cloning and quantitative real-time PCR (qPCR) were employed to characterize the rabbit GDF9 gene. By cloning, 2515-bp genomic DNA and 1359-bp cDNA sequences were obtained...
June 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28669387/amplification-and-selection-of-prrsv-activated-vdj-repertoires-in-pigs-secreting-distinct-neutralizing-antiboidies
#16
Nanhua Chen, Benjamin R Trible, Raymond R R Rowland
Neutralizing antibodies (nAbs) play an important role in protective immunity against porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, the characterization of PRRSV nAb repertoires is rarely investigated. In this study, we developed a swine VDJ amplification method and selection criteria for the characterization of PRRSV-activated VDJ repertoires. According to clonal expansion theory, two separated aliquots of lymph nodes from pigs producing different PRRSV nAbs were utilized to determine the activated B-cell repertoires...
July 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28650672/campylobacter-species-isolated-from-pigs-in-grenada-exhibited-novel-clones-genotypes-and-antimicrobial-resistance-profiles-of-sequence-types
#17
Victor A Amadi, Vanessa Matthew-Belmar, Charmarthy Subbarao, Isaac Kashoma, Gireesh Rajashekara, Ravindra Sharma, Harry Hariharan, Diana Stone
Infections caused by Campylobacter species pose a severe threat to public health worldwide. However, in Grenada, the occurrence and characteristics of Campylobacter in food animals, including pigs, remain mostly unknown. In this study, we identified the sequence types (STs) of Campylobacter from young healthy pigs in Grenada and compared the results with previous studies in Grenada and other countries. Antimicrobial resistance patterns and diversity of the Campylobacter clones were evaluated. Ninety-nine Campylobacter isolates (97 Campylobacter coli and 2 Campylobacter jejuni) were analyzed by multilocus sequence typing...
July 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28650390/derivation-and-characterization-of-a-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line
#18
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
OBJECTIVES: The aim of this study was to identify an epithelial cell line isolated from the spontaneous differentiation of totipotent pig epiblast cells. METHODS: PICM-31 and its colony-cloned derivative cell line, PICM-31A, were established from the culture and differentiation of an epiblast mass isolated from an 8-day-old pig blastocyst. The cell lines were analyzed by transmission electron microscopy, marker gene expression, and mass spectroscopy-based proteomics...
June 22, 2017: Pancreas
https://www.readbyqxmd.com/read/28644752/expression-and-purification-of-swine-rag2-in-e-coli-for-production-of-porcine-rag2-polyclonal-antibodies
#19
Yu-Bei Jin, Wen-Tao Yang, Ke-Yan Huang, Hong-Liang Chen, Seria-Masole Shonyela, Jing Liu, Qiong Liu, Bo Feng, You Zhou, Shu-Li Zhi, Yan-Long Jiang, Jian-Zhong Wang, Hai-Bin Huang, Chun-Wei Shi, Gui-Lian Yang, Chun-Feng Wang
Recombination activating gene 2 (RAG2) is necessary for immature B cell differentiation. Antibodies to human and rabbit RAG2 are currently commercially available, but antibodies to swine RAG remain unavailable to date. In this study, the swine RAG2 genes sequence was synthesized and then cloned into a pET-28a vector. The recombinant fusion protein was successfully expressed in E. coli, purified through nickel column chromatography, and further digested with Tobacco Etch Virus protease. The cleaved protein was purified by molecular-exclusion chromatography and named pRAG2...
August 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28638980/first-report-of-typical-brazilian-toxoplasma-gondii-genotypes-from-isolates-of-free-range-chickens-gallus-gallus-domesticus-circulating-in-the-state-of-para%C3%A3-ba-northeast-brazil
#20
Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, João Leite de Almeida-Neto, Lídio Ricardo Bezerra de Melo, Dayana Firmino de Morais, Bruna Farias Alves, Fabiana Nakashima, Solange Maria Gennari, Ana Célia Rodrigues Athayde, Hilda Fátima de Jesus Pena
This study evaluated, for the first time, the genetic diversity of Toxoplasma gondii isolates from free-range chickens from the state of Paraíba, Northeast Brazil. Tissue samples from 33 chickens from properties in five municipalities of Paraíba (Esperança, Olho d'Água, Malta, Monteiro, and Patos) were bioassayed in mice. The brains of mice infected with T. gondii cysts were used for DNA extraction and genotyping. Genotyping was performed using 11 PCR-RFLP markers and 15 microsatellite (MS) markers. Complete genotyping results were obtained for 29 isolates, with nine genotypes detected by RFLP and 15 genotypes identified by MS...
August 2017: Parasitology Research
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