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https://www.readbyqxmd.com/read/29452158/protein-kinase-c-epsilon-mediates-the-inhibition-of-angiotensin-ii-on-the-slowly-activating-delayed-rectifier-potassium-current-through-channel-phosphorylation
#1
Xiangbo Gou, Wenying Wang, Sihao Zou, Yajuan Qi, Yanfang Xu
The slowly activating delayed rectifier K+ current (IKs ) is one of the main repolarizing currents in the human heart. Evidence has shown that angiotensin II (Ang II) regulates IKs through the protein kinase C (PKC) pathway, but the related results are controversial. This study was designed to identify PKC isoenzymes involved in the regulation of IKs by Ang II and the underlying molecular mechanism. The whole-cell patch-clamp technique was used to record IKs in isolated guinea pig ventricular cardiomyocytes and in human embryonic kidney (HEK) 293 cells co-transfected with human KCNQ1/KCNE1 genes and Ang II type 1 receptor genes...
February 13, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29447173/dynamic-changes-of-setd2-a-histone-h3k36-methyltransferase-in-porcine-oocytes-ivf-and-scnt-embryos
#2
Yun Fei Diao, Tao Lin, Xiaoxia Li, Reza K Oqani, Jae Eun Lee, So Yeon Kim, Dong Il Jin
SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine fetal fibroblasts, oocytes, and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA), and somatic cell nuclear transfer (SCNT)...
2018: PloS One
https://www.readbyqxmd.com/read/29442225/feeder-cell-independent-culture-of-the-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line-picm-31
#3
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
The adaptation to feeder-independent growth of a pig embryonic stem cell-derived pancreatic cell line is described. The parental PICM-31 cell line, previously characterized as an exocrine pancreas cell line, was colony-cloned two times in succession resulting in the derivative cell line, PICM-31A1. PICM-31A1 cells were adapted to growth on a polymerized collagen matrix using feeder cell-conditioned medium and were designated PICM-31FF. Like the parental cells, the PICM-31FF cells were small and grew relatively slowly in closely knit colonies that eventually coalesced into a continuous monolayer...
February 13, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29431246/recombinant-apxiv-protein-enhances-protective-efficacy-against-actinobacillus-pleuropneumoniae-in-mice-and-pigs
#4
Hsing-Chieh Wu, Po-Hsien Yeh, Kai-Jen Hsueh, Wen-Jen Yang, Chun-Yen Chu
AIMS: Available bacterins, commercial or autogenous, for Actinobacillus pleuropneumoniae disease control have thus far shown debatable protective efficacy, and only in homologous challenges. Our study sought to determine whether the addition of reombinant protein ApxIV to the multi-component vaccine could enhance protection against homologous and heterologous challenge of A. pleuropneumoniae. METHODS AND RESULTS: The virulence of ApxI, ApxII, ApxIV, and OMP were cloned and expressed using a prokaryotic system; these recombinant proteins were combined with inactivated A...
February 12, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29423214/use-of-gene-editing-technology-to-introduce-targeted-modifications-in-pigs
#5
REVIEW
Junghyun Ryu, Randall S Prather, Kiho Lee
Pigs are an important resource in agriculture and serve as a model for human diseases. Due to their physiological and anatomical similarities with humans, pigs can recapitulate symptoms of human diseases, making them a useful model in biomedicine. However, in the past pig models have not been widely used partially because of the difficulty in genetic modification. The lack of true embryonic stem cells in pigs forced researchers to utilize genetic modification in somatic cells and somatic cell nuclear transfer (SCNT) to generate genetically engineered (GE) pigs carrying site-specific modifications...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29412739/mc1568-enhances-histone-acetylation-during-oocyte-meiosis-and-improves-development-of-somatic-cell-nuclear-transfer-embryos-in-pig
#6
Huili Wang, Wei Cui, Chunhua Meng, Jun Zhang, Yinxia Li, Yong Qian, Guangdong Xing, Dongmin Zhao, Shaoxian Cao
An increasing number of studies have revealed that histone deacetylase (HDAC) mediated histone deacetylation is important for mammalian oocyte development. However, nonselective HDAC inhibitors (HDACi) were applied in most studies; the precise functions of specific HDAC classes during meiosis are poorly defined. In this study, the class IIa-specific HDACi MC1568 was used to reveal a crucial role of class IIa HDACs in the regulation of histone deacetylation during porcine oocyte meiosis. Besides, the functions of HDACs and histone acetyltransferases in regulating the balance of histone acetylation/deacetylation were also confirmed during oocyte maturation...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29402432/type-i-interferon-suppression-negative-and-host-mrna-nuclear-retention-negative-mutation-in-nsp1%C3%AE-confers-attenuation-of-porcine-reproductive-and-respiratory-syndrome-virus-in-pigs
#7
Hanzhong Ke, Mingyuan Han, Qingzhan Zhang, Raymond Rowland, Maureen Kerrigan, Dongwan Yoo
Porcine reproductive and respiratory syndrome virus (PRRSV) has the ability to suppress the type I interferons (IFNs-α/β) induction to facilitate its survival during infection, and the nsp1 protein of PRRSV has been identified as the potent IFN antagonist. The nsp1β subunit of nsp1 has also been shown to block the host mRNA nuclear export as one of the mechanisms to suppress host antiviral protein production. The SAP motif in nsp1β is the functional motif for both IFN suppression and host mRNA nuclear retention, and using infectious clones, two mutant viruses vL126A and vL135A have been generated...
February 2, 2018: Virology
https://www.readbyqxmd.com/read/29392839/pharmacologic-treatment-of-donor-cells-induced-to-have-a-warburg-effect-like-metabolism-does-not-alter-embryonic-development-in-vitro-or-survival-during-early-gestation-when-used-in-somatic-cell-nuclear-transfer-in-pigs
#8
B R Mordhorst, S L Murphy, R M Ross, J A Benne, M S Samuel, R F Cecil, B K Redel, L D Spate, C N Murphy, K D Wells, J A Green, R S Prather
Somatic cell nuclear transfer is a valuable technique for the generation of genetically engineered animals, however the efficiency of cloning in mammalian species is low (1-3%). Differentiated somatic cells commonly used in nuclear transfer utilize the tricarboxylic acid cycle and cellular respiration for energy production. Comparatively the metabolism of somatic cells contrasts that of the cells within the early embryos which predominately use glycolysis. Early embryos (prior to implantation) are evidenced to exhibit characteristics of a Warburg Effect (WE)-like metabolism...
February 2, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29386289/immunisation-of-pigs-by-dna-prime-and-recombinant-vaccinia-virus-boost-to-identify-and-rank-african-swine-fever-virus-immunogenic-and-protective-proteins
#9
James K Jankovich, Dave Chapman, Debra T Hansen, Mark D Robida, Andrey Loskutov, Felicia Craciunescu, Alex Borovkov, Karen Kibler, Lynnette Goatley, Katherine King, Christopher L Netherton, Geraldine Taylor, Bertram Jacobs, Kathryn Sykes, Linda K Dixon
African swine fever virus (ASFV) causes an acute haemorrhagic fever in domestic pigs with high socio-economic impact. No vaccine is available limiting options for control. Although live attenuated ASFV can induce up to one hundred percent protection against lethal challenge, little is known of the antigens which induce this protective response. To identify additional ASFV immunogenic and potentially protective antigens we cloned 47 viral genes in individual plasmids for gene vaccination and in recombinant vaccinia viruses...
January 31, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29383175/apancreatic-pigs-cloned-using-pdx1-disrupted-fibroblasts-created-via-talen-mediated-mutagenesis
#10
Jin-Dan Kang, Hyojin Kim, Long Jin, Qing Guo, Cheng-Du Cui, Wen-Xue Li, Seokjoong Kim, Jin-Soo Kim, Xi-Jun Yin
Pancreatic and duodenal homeobox 1 (PDX1) plays a crucial role in pancreas development, β-cell differentiation, and maintenance of mature β-cell function. In this study, we designed a strategy to produce PDX1-knockout (KO) pigs. A transcription activator-like effector nuclease (TALEN) pair targeting exon 1 of the swine PDX1 gene was constructed. Porcine fetal fibroblasts (PFFs) were transfected with the TALEN plasmids plus a surrogate reporter plasmid. PDX1-mutated PFFs were enriched by magnetic separation and used to produce homozygous PDX1-KO pigs via a two-step somatic cell nuclear transfer (SCNT) cloning process...
December 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29362862/the-development-of-an-in-vitro-pig-a-assay-in-l5178y-cells
#11
Rhiannon David, Emily Talbot, Bethany Allen, Amy Wilson, Usman Arshad, Ann Doherty
A recent flow cytometry-based in vivo mutagenicity assay involves the hemizygous phosphatidylinositol class A (Pig-a) gene. Pig-a forms the catalytic subunit of N-acetylglucosaminyltransferase required for glycophosphatidylinositol (GPI) anchor biosynthesis. Mutations in Pig-a prevent GPI-anchor synthesis resulting in loss of cell-surface GPI-linked proteins. The aim of the current study was to develop and validate an in vitro Pig-a assay in L5178Y mouse lymphoma cells. Ethyl methanesulfonate (EMS)-treated cells (186...
January 23, 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/29337117/xist-derepression-in-active-x-chromosome-hinders-pig-somatic-cell-nuclear-transfer
#12
Degong Ruan, Jiangyun Peng, Xiaoshan Wang, Zhen Ouyang, Qingjian Zou, Yi Yang, Fangbing Chen, Weikai Ge, Han Wu, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Chengdan Lai, Nana Fan, Zhiwei Zhou, Qishuai Liu, Nan Li, Qin Jin, Hui Shi, Jingke Xie, Hong Song, Xiaoyu Yang, Jiekai Chen, Kepin Wang, Xiaoping Li, Liangxue Lai
Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, by profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome and identified a considerable number of genes that are aberrantly expressed in the abnormal cloned embryos. In particular, XIST, a long non-coding RNA gene, showed high ectopic expression in abnormal embryos. We also proved that nullification of the XIST gene in donor cells can normalize aberrant gene expression in cloned embryos and enhance long-term development capacity of the embryos...
January 10, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29324785/phylogenetic-diversity-antimicrobial-susceptibility-and-virulence-gene-profiles-of-brachyspira-hyodysenteriae-isolates-from-pigs-in-germany
#13
Jessica Joerling, Stefanie A Barth, Karen Schlez, Hermann Willems, Werner Herbst, Christa Ewers
Swine dysentery (SD) is an economically important diarrheal disease in pigs caused by different strongly hemolytic Brachyspira (B.) species, such as B. hyodysenteriae, B. suanatina and B. hampsonii. Possible associations of epidemiologic data, such as multilocus sequence types (STs) to virulence gene profiles and antimicrobial susceptibility are rather scarce, particularly for B. hyodysenteriae isolates from Germany. In this study, B. hyodysenteriae (n = 116) isolated from diarrheic pigs between 1990 and 2016 in Germany were investigated for their STs, susceptibility to the major drugs used for treatment of SD (tiamulin and valnemulin) and genes that were previously linked with virulence and encode for hemolysins (tlyA, tlyB, tlyC, hlyA, BHWA1_RS02885, BHWA1_RS09085, BHWA1_RS04705, and BHWA1_RS02195), outer membrane proteins (OMPs) (bhlp16, bhlp17...
2018: PloS One
https://www.readbyqxmd.com/read/29322645/faecal-escherichia-coli-as-biological-indicator-of-spatial-interaction-between-domestic-pigs-and-wild-boar-sus-scrofa-in-corsica
#14
S A Barth, S Blome, D Cornelis, J Pietschmann, M Laval, O Maestrini, L Geue, F Charrier, E Etter, C Menge, M Beer, F Jori
On the Mediterranean island of Corsica, cohabitation between sympatric domestic pigs and Eurasian wild boar (Sus scrofa) is common and widespread and can facilitate the maintenance and dissemination of several pathogens detrimental for the pig industry or human health. In this study, we monitored a population of free-ranging domestic pigs reared in extensive conditions within a 800-ha property located in Central Corsica which was frequently visited by a sympatric population of wild boar between 2013 and 2015...
January 10, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29321316/two-residues-in-nsp9-contribute-to-the-enhanced-replication-and-pathogenicity-of-highly-pathogenic-porcine-reproductive-and-respiratory-syndrome-virus
#15
Kuan Zhao, Jia-Cong Gao, Jun-Yao Xiong, Jin-Chao Guo, Yong-Bo Yang, Cheng-Gang Jiang, Yan-Dong Tang, Zhi-Jun Tian, Xue-Hui Cai, Guang-Zhi Tong, Tong-Qing An
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) possesses greater replicative capacity and pathogenicity than classical PRRSV. However, the factors that lead to enhanced replication and pathogenicity remain unclear. In our study, an alignment of all available full-length sequences of North-American type PRRSVs (n = 204) revealed two consistent amino acid mutations that differed between HP-PRRSV and classical PRRSV and were located at positions 519 and 544 in non-structural protein 9...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29311328/modeling-lethal-x-linked-genetic-disorders-in-pigs-with-ensured-fertility
#16
Hitomi Matsunari, Masahito Watanabe, Kazuaki Nakano, Shin Enosawa, Kazuhiro Umeyama, Ayuko Uchikura, Sayaka Yashima, Toru Fukuda, Nikolai Klymiuk, Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Valeri Zakhartchenko, Eckhard Wolf, Yutaka Hanazono, Masaki Nagaya, Akihiro Umezawa, Hiromitsu Nakauchi, Hiroshi Nagashima
Genetically engineered pigs play an indispensable role in the study of rare monogenic diseases. Pigs harboring a gene responsible for a specific disease can be efficiently generated via somatic cell cloning. The generation of somatic cell-cloned pigs from male cells with mutation(s) in an X chromosomal gene is a reliable and straightforward method for reproducing X-linked genetic diseases (XLGDs) in pigs. However, the severe symptoms of XLGDs are often accompanied by impaired growth and reproductive disorders, which hinder the reproduction of these valuable model animals...
January 8, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29302255/species-specific-antiviral-activity-of-porcine-interferon-%C3%AE-8-ifn%C3%AE-8
#17
Eunhye Kim, Hyunjhung Jhun, Joohee Kim, Unjoo Park, Seunghyun Jo, Areum Kwak, Sinae Kim, Tam T Nguyen, Yongsun Kang, Insoo Choi, Joongbok Lee, Heijun Kim, Younghyun Kim, Siyoung Lee, Soohyun Kim
Interferons (IFNs) have been known as antiviral genes and they are classified by type 1, type 2, and type 3 IFN. The type 1 IFN consists of IFNα, IFNβ, IFNτ, and IFNω whereas the type 2 IFN consists of only IFNγ, which is a key cytokine driving T helper cell type 1 immunity. IFNλ belongs to the type 3 IFN, which is also known as IL-28 and IL-29 possessing antiviral activities. Type 1 IFN is produced by viral infection whereas type 2 IFN is induced by mitogenic or antigenic T-cell stimuli. The IFNτ of bovine was first discovered in an ungulate ruminant recognition hormone...
December 2017: Immune Network
https://www.readbyqxmd.com/read/29273286/genetic-analysis-of-virulence-and-antimicrobial-resistant-plasmid-pou7519-in-salmonella-enterica-serovar-choleraesuis
#18
Chyi-Liang Chen, Lin-Hui Su, Rajendra Prasad Janapatla, Chun-Yen Lin, Cheng-Hsun Chiu
BACKGROUND: Zoonotic Salmonella enterica serovar Choleraesuis (S. Choleraesuis), causing paratyphoid in pigs and bacteremia in humans, commonly carry a virulence plasmid and sometimes a separate antimicrobial-resistant plasmid or merging together. This study aimed to analyze the likely mechanism of how to form a virulence-resistance chimera of plasmid in S. Choleraesuis. METHODS: Whole plasmid sequence of pOU7519 in S. Choleraesuis strain OU7519 was determined using shotgun cloning and sequencing...
December 14, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29249323/characterization-and-comparative-analysis-of-immunoglobulin-lambda-chain-diversity-in-a-neonatal-porcine-model
#19
Nannan Guo, Menghan Su, Zicong Xie, Kankan Wang, Hongming Yuan, Mengjing Li, Jianing Li, Minghao Liu, Jing Bai, Jing Liu, Hongsheng Ouyang, Daxin Pang, Huping Jiao
To elucidate how antigen exposure and selection shape the porcine antibody repertoires, we investigated the immunoglobulin lambda light chain (IGL) gene repertoires of the binary cross-bred (Yorkshire×Landrace) pig at different developmental stages, pre-suckle neonate (0days), wean piglet (35days) and growing pig (75days) under normal farming conditions. Immunoglobulin lambda light transcript (IGLV-J-C) clones of the peripheral blood mononuclear cells (PBMCs) from these different developmental stages were assessed for IGL combination, junction and sequence diversity...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29208927/establishment-of-a-strain-of-haemophilia-a-pigs-by-xenografting-of-foetal-testicular-tissue-from-neonatally-moribund-cloned-pigs
#20
Hiroyuki Kaneko, Kazuhiro Kikuchi, Michiko Nakai, Daiichiro Fuchimoto, Shunichi Suzuki, Shoichiro Sembon, Junko Noguchi, Akira Onishi
Grafting of testicular tissue into immunodeficient mice makes it possible to obtain functional sperm from immature donor animals that cannot be used for reproduction. We have developed a porcine model of human haemophilia A (haemophilia-A pigs) by nuclear transfer cloning from foetal fibroblasts after disruption of the X-linked coagulation factor VIII (F8) gene. Despite having a recessive condition, female F8+/- cloned pigs died of severe bleeding at an early age, as was the case for male F8-/Y cloned pigs, thus making it impossible to obtain progeny...
December 5, 2017: Scientific Reports
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