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https://www.readbyqxmd.com/read/29624021/construction-of-a-recombinant-baculovirus-expressing-swine-hepatitis-e-virus-orf2-and-preliminary-research-on-its-immune-effect
#1
Z Yang, Y Hu, P Yuan, Y Yang, K Wang, L Y Xie, S L Huang, J Liu, L Ran, Z H Song
In the swine hepatitis E virus (HEV), open reading frame 2 (ORF2) is rich in antigenic determinants and neutralizing epitopes that could induce immune protection. We chose the Bac-to-Bac® Baculovirus Expression System to express fragments containing the critical neutralizing antigenic sites within the HEV ORF2 protein of pigs to obtain a recombinant baculovirus. The fragment of swine HEV ORF2 region (1198-1881bp) was cloned into vector pFastBacTM. A recombinant baculovirus, rBacmid-ORF2, was obtained after transposition and transfection...
March 2018: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29621585/cloning-identification-and-functional-analysis-of-bone-marrow-stromal-cell-antigen-2-from-sika-deer-cervus-nippon
#2
Jiawen Wang, Shuai Bian, Meichun Liu, Xin Zhang, Siming Wang, Xueyuan Bai, Daqing Zhao, Yu Zhao
BST-2(tetherin/CD317/HM1.24) has been identified as a cellular antiviral factor that inhibits the release of a wide range of enveloped viruses from infected cells. Orthologs of BST-2 have been identified in several species including humans, monkeys, cows, sheep, pigs, and mice. In this study, we cloned and characterized the protein of the BST-2 homolog from sika deer (Cervus nippon). cnBST-2 shares 37.8% and 74.2% identity with the BST-2 homologs from Homo sapiens and Ovis aries, respectively. The extracellular domain of cnBST-2 has two putative N-linked glycosylation sites and three potential dimerization sites...
April 2, 2018: Gene
https://www.readbyqxmd.com/read/29620444/cloning-of-buffalo-a-highly-valued-livestock-species-of-south-and-southeast-asia-any-achievements
#3
Naresh L Selokar, Monika Saini, Prabhat Palta, Manmohan S Chauhan, Radhey S Manik, Suresh K Singla
Buffalo (Bubalus bubalis) is a major source of milk, meat, and draught power in many developing countries in Asia. Animal cloning holds a lot of potential for fast multiplication of elite buffaloes and conservation of their valuable germplasm. Although the progress of buffalo cloning has been slow in comparison to cattle or pig, several breakthroughs were reported in buffalo cloning such as the production of cloned calves from somatic cells isolated from over one-decade old frozen-thawed semen or from urine-derived cells...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29601977/surf1-knockout-cloned-pigs-early-onset-of-a-severe-lethal-phenotype
#4
C Quadalti, D Brunetti, I Lagutina, R Duchi, A Perota, G Lazzari, R Cerutti, I Di Meo, M Johnson, E Bottani, P Crociara, C Corona, S Grifoni, V Tiranti, E Fernandez-Vizarra, A J Robinson, C Viscomi, C Casalone, M Zeviani, C Galli
Leigh syndrome (LS) associated with cytochrome c oxidase (COX) deficiency is an early onset, fatal mitochondrial encephalopathy, leading to multiple neurological failure and eventually death, usually in the first decade of life. Mutations in SURF1, a nuclear gene encoding a mitochondrial protein involved in COX assembly, are among the most common causes of LS. LSSURF1 patients display severe, isolated COX deficiency in all tissues, including cultured fibroblasts and skeletal muscle. Recombinant, constitutive SURF1-/- mice show diffuse COX deficiency, but fail to recapitulate the severity of the human clinical phenotype...
March 27, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29577951/interaction-of-porcine-reproductive-and-respiratory-syndrome-virus-major-envelope-proteins-gp5-and-m-with-the-cellular-protein-snapin
#5
Julie A Hicks, Dongwan Yoo, Hsiao-Ching Liu
BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) is characterized by abortions in pregnant sows and respiratory disease, particularly in young pigs. The causative agent is porcine reproductive and respiratory syndrome virus (PRRSV), a member of the arterivirus family. GP5 and M are the major envelope proteins encoded by PRRSV. To further characterize these two viral proteins, a yeast two-hybrid approach was utilized to identify interacting partners of PRRSV GP5 and M proteins...
March 22, 2018: Virus Research
https://www.readbyqxmd.com/read/29575852/streptococcus-suis-a-re-emerging-pathogen-associated-with-occupational-exposure-to-pigs-or-pork-products-part-ii-pathogenesis
#6
Jacek Dutkiewicz, Violetta Zając, Jacek Sroka, Bernard Wasiński, Ewa Cisak, Anna Sawczyn, Anna Kloc, Angelina Wójcik-Fatla
<i>Streptococcus suis</i> is a re-emerging zoonotic pathogen that may cause severe disease, mostly meningitis, in pigs and in humans having occupational contact with pigs and pork, such as farmers, slaughterhose workers and butchers. The first stage of the pathogenic process, similar in pigs and humans, is adherence to and colonisation of mucosal and/or epithelial surface(s) of the host. The second stage is invasion into deeper tissue and extracellular translocation of bacterium in the bloodstream, either free in circulation or attached to the surface of monocytes...
March 14, 2018: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/29572319/ossabaw-pigs-with-a-pcsk9-gain-of-function-mutation-develop-accelerated-coronary-atherosclerotic-lesions-a-novel-model-for-preclinical-studies
#7
Fang Yuan, Liang Guo, Kyoung-Ha Park, John R Woollard, Kwon Taek-Geun, Kai Jiang, Tamene Melkamu, Bin Zang, Samantha L Smith, Scott C Fahrenkrug, Frank D Kolodgie, Amir Lerman, Renu Virmani, Lilach O Lerman, Daniel F Carlson
BACKGROUND: Ossabaw pigs are unique miniature swine with genetic predisposition to develop metabolic syndrome and coronary atherosclerosis after extended periods receiving atherogenic diets. We have hypothesized that transgenic Ossabaw swine expressing chimp PCSK9 (proprotein convertase subtilisin-like/kexin type 9) containing the D374Y gain of function would develop familial hypercholesterolemia and coronary artery plaques more rapidly than Landrace swine with the same transgene. METHODS AND RESULTS: Ossabaw and Landrace PCSK9 gain-of-function founders were generated by Sleeping Beauty transposition and cloning...
March 23, 2018: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29556895/efficient-genome-editing-in-cultured-cells-and-embryos-of-debao-pig-and-swamp-buffalo-using-the-crispr-cas9-system
#8
Xiaoping Su, Kuiqing Cui, Shanshan Du, Hongli Li, Fenghua Lu, Deshun Shi, Qingyou Liu
Myostatin (MSTN), a protein encoded by growth differentiation factor 8 (GDF8), is primarily expressed in skeletal muscle and negatively regulates the development and regeneration of muscle. Accordingly, myostatin-deficient animals exhibit a double-muscling phenotype. The CRISPR/Cas9 system has proven to be an efficient genome-editing tool and has been applied to gene modification in cells from many model organisms such as Drosophila melanogaster, zebrafish, mouse, rat, sheep, and human. Here, we edited the GDF8 gene in fibroblasts and embryos of Debao pig and swamp buffalo using the CRISPR/Cas9 system...
March 19, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29555974/successful-knock-in-of-hypertrophic-cardiomyopathy-mutation-r723g-into-the-myh7-gene-mimics-hcm-pathology-in-pigs
#9
J Montag, B Petersen, A K Flögel, E Becker, A Lucas-Hahn, G J Cost, C Mühlfeld, T Kraft, H Niemann, B Brenner
Familial Hypertrophic Cardiomyopathy (HCM) is the most common inherited cardiac disease. About 30% of the patients are heterozygous for mutations in the MYH7 gene encoding the ß-myosin heavy chain (MyHC). Hallmarks of HCM are cardiomyocyte disarray and hypertrophy of the left ventricle, the symptoms range from slight arrhythmias to sudden cardiac death or heart failure. To gain insight into the underlying mechanisms of the diseases' etiology we aimed to generate genome edited pigs with an HCM-mutation. We used TALEN-mediated genome editing and successfully introduced the HCM-point mutation R723G into the MYH7 gene of porcine fibroblasts and subsequently cloned pigs that were heterozygous for the HCM-mutation R723G...
March 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29553062/the-dynamics-of-a-chinese-porcine-g9p-23-rotavirus-production-in-ma-104-cells-and-intestines-of-3-day-old-piglets
#10
Zhiyan Wang, Chaochao Lv, Xin Xu, Xiangdong Li, Yali Yao, Xiaojing Gao, Zhe Sun, Yuzhou Wang, Yujie Sun, Yan Xiao, Kegong Tian
Rotavirus A (RVA) G9 genotype is recognized as an emerging genotype which is spreading worldwide, however, our knowledge on pathogenicity of this virus is limited. In this study, porcine RVA strain HN03 was successfully isolated on MA-104 cells, and the isolate was propagated continuously for 7 passages after a virus cloning at passage 3. The virus titers from 4 to 10 passages ranged from 107.1 to 108.1 TCID50 /ml. The growth curve of HN03 strain in cell culture was determined, and the virus production dynamics was confirmed by immunoperoxidase monolayer assay (IPMA)...
March 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29516261/therapeutic-potential-of-bama-miniature-pig-adipose-stem-cells-induced-hepatocytes-in-a-mouse-model-with-acute-liver-failure
#11
Shuang Zhang, Zhiqiang Zhu, Yufeng Wang, Shi Liu, Chenqiong Zhao, Weijun Guan, Yuhua Zhao
The role of mesenchymal stem cells (MSCs) in cellular therapy is well recognized in this work. MSCs have advantages of high proliferation, clone formation, multi-lineage differentiation and immunosuppression. Furthermore, adipose-resident MSCs (ADSCs) are extensively employed due to its advantages of abundant source, low cost and simple operation. Many researchers have emphasized the role of adipose-resident MSCs in the development of therapies for liver injury, but few attentions were paid on the use of induced functional hepatocytes...
March 7, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29496526/the-correlation-between-the-mutual-deletions-of-amino-acids-within-porcine-circovirus-rep-protein-and-the-discrepancy-of-replication
#12
Lingling Zhang, Xiaoyan Wu, Jianli Shi, Zhe Peng, Shuxuan Zheng, Shengnan Xu, Hong Han, Changxun Xin, Yuwei Liu, Mei Gao, Jiang Yu, Wenbo Sun, Xiaoyan Cong, Jun Li, Shaojian Xu, Jinbao Wang
Porcine circovirus (PCV) has two potential open reading frames, ORF1 and ORF2. ORF1 is predicted to encode a replication-associated protein (Rep) essential for replication of viral DNA. In some studies, PCV1 replicated more efficiently in PK-15 cells than PCV2 was elucidated. PCV1 compared with PCV2; there is some amino acids' deficiency on Rep protein. To identify whether the above amino acids deletion affects the replication of PCV1 and PCV2, we constructed three double copy clones by overlap extension PCR...
February 26, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29489438/isolation-of-peripheral-blood-cd8-t-cells-specific-to-porcine-reproductive-and-respiratory-syndrome-virus-utilizing-porcine-cd137-activation-marker
#13
Sakthivel Subramaniam, Christopher Overend, Danielle M Yugo, C Lynn Heffron, Shannon R Matzinger, Adam J Rogers, Debin Tian, Qian M Cao, Scott P Kenney, Xiang-Jin Meng
CD137 is a costimulatory molecule transiently expressed on activated T cells after mitogen or antigen stimulation that can be exploited for isolating antigen-specific T cells as reported in mouse models. By utilizing an antiporcine CD137 monoclonal antibody (mAb, clone 3B9) developed in our laboratory, we isolated virus-specific CD8β T cells from peripheral blood of pigs experimentally infected with different porcine reproductive and respiratory syndrome virus (PRRSV) strains. Similar to mouse, porcine CD8β T cells also express CD137 transiently upon Concavalin A stimulation while the unstimulated cells did not...
February 28, 2018: Viral Immunology
https://www.readbyqxmd.com/read/29482569/generation-of-ghr-modified-pigs-as-laron-syndrome-models-via-a-dual-sgrnas-cas9-system-and-somatic-cell-nuclear-transfer
#14
Honghao Yu, Weihu Long, Xuezeng Zhang, Kaixiang Xu, Jianxiong Guo, Heng Zhao, Honghui Li, Yubo Qing, Weirong Pan, Baoyu Jia, Hong-Ye Zhao, Xingxu Huang, Hong-Jiang Wei
BACKGROUND: Laron syndrome is an autosomal disease resulting from mutations in the growth hormone receptor (GHR) gene. The only therapeutic treatment for Laron syndrome is recombinant insulin-like growth factor I (IGF-I), which has been shown to have various side effects. The improved Laron syndrome models are important for better understanding the pathogenesis of the disease and developing corresponding therapeutics. Pigs have become attractive biomedical models for human condition due to similarities in anatomy, physiology, and metabolism relative to humans, which could serve as an appropriate model for Laron syndrome...
February 27, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29481768/cloning-and-mrna-expression-of-npb-and-its-effect-on-hormone-secretion-of-the-reproductive-cells-in-the-pig
#15
Lei Zhihai
Neuropeptide B (NPB) is an endogenous ligand for the orphan G protein-coupled receptors NPBWR1 (GPR7) and NPBWR2 (GPR8). Some reports have investigated the role of NPB in the regulation of feeding, energy metabolism and hormone secretion in many species. However, few papers reported the physiological function of NPB in the pig. In this study, we cloned and sequenced the NPB mRNA from a pig, which was found to consist of 123 bases. NPB mRNA expression was detected in central and peripheral tissues by the quantitative fluorescence method...
February 23, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29479347/genome-sequencing-of-extended-spectrum-%C3%AE-lactamase-esbl-producing-klebsiella-pneumoniae-isolated-from-pigs-and-abattoir-workers-in-cameroon
#16
Luria L Founou, Raspail C Founou, Mushal Allam, Arshad Ismail, Cyrille F Djoko, Sabiha Y Essack
Background and objectives: Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae is a serious public health issue globally. In this study, the antibiotic resistance genes, virulence factors, mobile genetic elements, and genetic lineages of circulating ESBL-producing K. pneumoniae strains isolated from pigs and humans in Cameroonian abattoirs were investigated using whole genome sequencing (WGS), in order to ascertain zoonotic transmission (viz. from animals to humans and/or vice-versa) in the food chain...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29470680/changes-in-skeletal-muscle-and-body-weight-on-sleeping-beauty-transposon-mediated-transgenic-mice-overexpressing-pig-migf-1
#17
Bo Gao, Wei Wang, Han Wu, Cai Chen, Dan Shen, Saisai Wang, Wei Chen, Li Zhang, Shuheng Chan, Chengyi Song
Insulin-like growth factor (IGF-I) is an important growth factor in mammals, but the functions of the local muscle-specific isoform of insulin-like growth factor 1 (mIGF-1) to skeletal muscle development have rarely been reported. To determine the effect of pig mIGF-1 on body development and muscle deposition in vivo and to investigate the molecular mechanisms, the transgenic mouse model was generated which can also provide experimental data for making transgenic pigs with pig endogenous IGF1 gene. We constructed a skeletal muscle-specific expression vector using 5'- and 3'-regulatory regions of porcine skeletal α-actin gene...
February 22, 2018: Biochemical Genetics
https://www.readbyqxmd.com/read/29452158/protein-kinase-c-epsilon-mediates-the-inhibition-of-angiotensin-ii-on-the-slowly-activating-delayed-rectifier-potassium-current-through-channel-phosphorylation
#18
Xiangbo Gou, Wenying Wang, Sihao Zou, Yajuan Qi, Yanfang Xu
The slowly activating delayed rectifier K+ current (IKs ) is one of the main repolarizing currents in the human heart. Evidence has shown that angiotensin II (Ang II) regulates IKs through the protein kinase C (PKC) pathway, but the related results are controversial. This study was designed to identify PKC isoenzymes involved in the regulation of IKs by Ang II and the underlying molecular mechanism. The whole-cell patch-clamp technique was used to record IKs in isolated guinea pig ventricular cardiomyocytes and in human embryonic kidney (HEK) 293 cells co-transfected with human KCNQ1/KCNE1 genes and Ang II type 1 receptor genes...
March 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29447173/dynamic-changes-of-setd2-a-histone-h3k36-methyltransferase-in-porcine-oocytes-ivf-and-scnt-embryos
#19
Yun Fei Diao, Tao Lin, Xiaoxia Li, Reza K Oqani, Jae Eun Lee, So Yeon Kim, Dong Il Jin
SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine fetal fibroblasts, oocytes, and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA), and somatic cell nuclear transfer (SCNT)...
2018: PloS One
https://www.readbyqxmd.com/read/29442225/feeder-cell-independent-culture-of-the-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line-picm-31
#20
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
The adaptation to feeder-independent growth of a pig embryonic stem cell-derived pancreatic cell line is described. The parental PICM-31 cell line, previously characterized as an exocrine pancreas cell line, was colony-cloned two times in succession resulting in the derivative cell line, PICM-31A1. PICM-31A1 cells were adapted to growth on a polymerized collagen matrix using feeder cell-conditioned medium and were designated PICM-31FF. Like the parental cells, the PICM-31FF cells were small and grew relatively slowly in closely knit colonies that eventually coalesced into a continuous monolayer...
February 13, 2018: In Vitro Cellular & Developmental Biology. Animal
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