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https://www.readbyqxmd.com/read/28546489/genome-sequence-of-a-marine-spirillum-oceanospirillum-multiglobuliferum-atcc-33336-t-isolated-from-japan
#1
Joshua G Carney, Ariel M Trachtenberg, Bruce A Rheaume, Joshua D Linnane, Natalie L Pitts, Donald L Mykles, Kyle S MacLea
Oceanospirillum multiglobuliferum ATCC 33336(T) is a motile gammaproteobacterium with bipolar tufted flagella, noted for its low salt tolerance compared to other marine spirilla. This strain was originally isolated from the putrid infusions of Crassostrea gigas near Hiroshima, Japan. This paper presents a draft genome sequence for O. multiglobuliferum ATCC 33336(T).
May 25, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28544270/localization-and-domain-characterization-of-the-sfla-regulator-of-flagellar-formation-in-vibrio-alginolyticus
#2
Satoshi Inaba, Takehiko Nishigaki, Norihiro Takekawa, Seiji Kojima, Michio Homma
Many swimming bacteria use flagella as locomotive organelles. The spatial and numerical regulation of flagellar biosynthesis differs by bacterial species. The marine bacteria Vibrio alginolyticus use a single polar flagellum whose number is regulated positively by FlhF and negatively by FlhG. Cells lacking FlhF and FlhG have no flagellum. The motility defect in an flhFG deletion was suppressed by a mutation in the sflA gene that resulted in the production of multiple, peritrichous flagella. SflA is a Vibrio-specific protein...
May 22, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28541401/three-dimensional-structural-labeling-microscopy-of-cilia-and-flagella
#3
Toshiyuki Oda
Locating a molecule within a cell using protein-tagging and immunofluorescence is a fundamental technique in cell biology, whereas in three-dimensional electron microscopy, locating a subunit within a macromolecular complex remains challenging. Recently, we developed a new structural labeling method for cryo-electron tomography by taking advantage of the biotin-streptavidin system, and have intensively used this method to locate a number of proteins and protein domains in cilia and flagella. In this review, we summarize our findings on the three-dimensional architecture of the axoneme, especially the importance of coiled-coil proteins...
May 25, 2017: Microscopy
https://www.readbyqxmd.com/read/28537545/paenibacillus-lacus-sp-nov-isolated-from-a-water-reservoir
#4
Wen-Ming Chen, Kai-Rou Lin, Shih-Yi Sheu
A bacterial strain, designated Agd-32T, was isolated from a water sample taken from the Agongdian Reservoir in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain Agd-32T were Gram-stain-variable, aerobic, motile by means of peritrichous flagella, endospore-forming, rod-shaped and surrounded by a thick capsule. Strain Agd-32T contained anteiso-C15 : 0 and C16 : 0 as the predominant fatty acids. The major isoprenoid quinone was MK-7, and the polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophosphoglycolipid, one unidentified glycolipid, one unidentified phospholipid and one unidentified lipid...
May 25, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28536199/active-depinning-of-bacterial-droplets-the-collective-surfing-of-bacillus-subtilis
#5
Marc Hennes, Julien Tailleur, Gaëlle Charron, Adrian Daerr
How systems are endowed with migration capacity is a fascinating question with implications ranging from the design of novel active systems to the control of microbial populations. Bacteria, which can be found in a variety of environments, have developed among the richest set of locomotion mechanisms both at the microscopic and collective levels. Here, we uncover, experimentally, a mode of collective bacterial motility in humid environment through the depinning of bacterial droplets. Although capillary forces are notoriously enormous at the bacterial scale, even capable of pinning water droplets of millimetric size on inclined surfaces, we show that bacteria are able to harness a variety of mechanisms to unpin contact lines, hence inducing a collective slipping of the colony across the surface...
May 23, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28534617/comparative-proteomics-reveals-timely-transport-into-cilia-of-regulators-or-effectors-as-a-mechanism-underlying-ciliary-disassembly
#6
Limei Wang, Lixiao Gu, Dan Meng, Qiong Wu, Haiteng Deng, Junmin Pan
Primary cilia are assembled and disassembled during cell cycle progression. During ciliary disassembly, ciliary axonemal microtubules (MTs) are depolymerized accompanied with extensive posttranslational protein modifications of ciliary proteins including protein phosphorylation, methylation and ubiquitination. These events are hypothesized to involve transport of effectors or regulators into cilia at the time of ciliary disassembly from the cell body. To prove this hypothesis and identify new proteins involved in ciliary disassembly, we analyzed disassembling flagella in Chlamydomonas using comparative proteomics with TMT labeling...
May 23, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28533336/a-coding-variant-in-the-gene-bardet-biedl-syndrome-4-bbs4-is-associated-with-a-novel-form-of-canine-progressive-retinal-atrophy
#7
Tracy Chew, Bianca Haase, Roslyn Bathgate, Cali E Willet, Maria K Kaukonen, Lisa J Mascord, Hannes T Lohi, Claire M Wade
Progressive retinal atrophy is a common cause of blindness in the dog and affects over 100 breeds. It is characterized by gradual vision loss that occurs due to the degeneration of photoreceptor cells in the retina. Similar to the human counterpart retinitis pigmentosa, the canine disorder is clinically and genetically heterogeneous and the underlying cause remains unknown for many cases. We use a positional candidate gene approach to identify putative variants in the Hungarian Puli breed using genotyping data of 14 family-based samples (CanineHD BeadChip array, Illumina) and whole genome sequencing data of two proband and two parental samples (Illumina HiSeq 2000)...
May 22, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28533217/transcriptional-control-of-the-lateral-flagellar-genes-of-bradyrhizobium-diazoefficiens
#8
Elías J Mongiardini, J Ignacio Quelas, Carolina Dardis, M Julia Althabegoiti, Aníbal R Lodeiro
Bradyrhizobium diazoefficiens, the soybean N2-fixing symbiont, possesses a dual flagellar system comprising a constitutive subpolar flagellum and inducible lateral flagella. Here, we analyzed the genomic organization and biosynthetic regulation of the lateral-flagellar genes. We found that those genes are located in a single genomic cluster, organized in two monocistronic transcriptional units and three operons, one possibly containing an internal transcription start site. Among the monocistronic units is blr6846, homologous to the Class-IB master regulators of flagella synthesis in Brucella melitensis and Ensifer meliloti, and required for the expression of all the lateral-flagellar genes except lafA2, which locus encodes a single lateral flagellin...
May 22, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28522292/cross-talk-between-bacterial-two-component-systems-drives-stepwise-regulation-of-flagellar-biosynthesis-in-swarming-development
#9
Chia-Fong Wei, Yu-Huan Tsai, Sheng-Hui Tsai, Chuan-Sheng Lin, Chih-Jung Chang, Chia-Chen Lu, Hsiou-Chen Huang, Hsin-Chih Lai
Swarming motility is a mode of bacterial movement over a solid surface driven by rotating flagella in a coordinated manner. Bacteria can use two-component system (TCS), which typically comprises a sensor kinase and a specific cognate response regulator, to properly react to environmental changes. We previously showed that the TCS RssAB suppresses flagellar biosynthesis master regulator flhDC specifically in swarming lag phase to control surface migration timing without affecting expansion rate in Serratia marcescens swarming development...
May 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28515773/building-a-multipurpose-insertional-mutant-library-for-forward-and-reverse-genetics-in-chlamydomonas
#10
Xi Cheng, Gai Liu, Wenting Ke, Lijuan Zhao, Bo Lv, Xiaocui Ma, Nannan Xu, Xiaoling Xia, Xuan Deng, Chunlei Zheng, Kaiyao Huang
BACKGROUND: The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system, is not widely used in this organism. Screening of random insertional mutant libraries by polymerase chain reaction provides an alternate strategy to obtain null mutants of individual gene. But building, screening, and maintaining such a library was time-consuming and expensive...
2017: Plant Methods
https://www.readbyqxmd.com/read/28513721/programmable-micrometer-sized-motor-array-based-on-live-cells
#11
Shuangxi Xie, Xiaodong Wang, Niandong Jiao, Steve Tung, Lianqing Liu
Trapping and transporting microorganisms with intrinsic motility are important tasks for biological, physical, and biomedical applications. However, fast swimming speed makes the manipulation of these organisms an inherently challenging task. In this study, we demonstrated that an optoelectrical technique, namely, optically induced dielectrophoresis (ODEP), could effectively trap and manipulate Chlamydomonas reinhardtii (C. reinhardtii) cells swimming at velocities faster than 100 μm s(-1). Furthermore, live C...
May 17, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28513418/flagellar-expression-in-clinical-isolates-of-non-typeable-haemophilus-influenzae
#12
Alejandro Carabarin-Lima, Patricia Lozano-Zarain, Miguel Castañeda-Lucio, Claudia Fabiola Martínez de la Peña, Julieta Martinez-Garcia, Norarizbeth Lara Flores, Elías Campos de la Cruz, Sirenia González-Posos, Rosa Del Carmen Rocha-Gracia
PURPOSE: Haemophilus influenzae is a commensal organism found in the upper respiratory tract of humans. When H. influenzae becomes a pathogen, these bacteria can move out of their commensal niche and cause multiple respiratory tract diseases such as otitis media, sinusitis, conjunctivitis and bronchitis in children, and chronic obstructive pulmonary disease in adults. However, H. influenzae is currently considered a non-flagellate bacterium. METHODOLOGY AND RESULTS: In this study, 90 clinical isolates of H...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28505375/between-a-pod-and-a-hard-test-the-deep-evolution-of-amoebae
#13
Seungho Kang, Alexander K Tice, Frederick W Spiegel, Jeffrey D Silberman, Tomáš Pánek, Ivan Cepicka, Martin Kostka, Anush Kosakyan, Daniel M Alcântara, Andrew J Roger, Lora L Shadwick, Alexey Smirnov, Alexander Kudryavstev, Daniel J G Lahr, Matthew W Brown
Amoebozoa is the eukaryotic supergroup sister to Obazoa, the lineage that contains the animals and Fungi, as well as their protistan relatives, and the breviate and apusomonad flagellates. Amoebozoa is extraordinarily diverse, encompassing important model organisms and significant pathogens. Although amoebozoans are integral to global nutrient cycles and present in nearly all environments, they remain vastly understudied. We present a robust phylogeny of Amoebozoa based on broad representative set of taxa in a phylogenomic framework (325 genes)...
May 15, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28505094/antigiardial-activity-of-podophyllotoxin-type-lignans-from-bursera-fagaroides-var-fagaroides
#14
Filiberto Gutiérrez-Gutiérrez, Ana María Puebla-Pérez, Sirenia González-Pozos, José Manuel Hernández-Hernández, Armando Pérez-Rangel, Laura Patricia Alvarez, Gabriela Tapia-Pastrana, Araceli Castillo-Romero
Giardiasis, a diarrheal disease, is highly prevalent in developing countries. Several drugs are available for the treatment of this parasitosis; unfortunately, all of them have variable efficacies and adverse effects. Bursera fagaroides has been known for its anti-inflammatory and antidiarrheal properties in Mexican traditional medicine. We investigated the in vitro anti-giardial activities of four podophyllotoxin-type lignans from Bursera fagaroides var. fagaroides, namely, 5'-desmethoxy-β-peltatin-A-methylether (5-DES), acetylpodophyllotoxin (APOD), burseranin (BUR), and podophyllotoxin (POD)...
May 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28502296/-recombinant-legionella-pneumophila-flagella-protein-a-rflaa-induces-the-secretion-of-il-6-and-il-1%C3%AE-in-raw264-7-cells-in-vitro
#15
Lijuan Jia, Xiuqin Cao, Zhiwei Yang
Objective To investigate the effect of recombinant Legionella pneumophila flagella protein A (rflaA) on the secretion of interleukin-6 (IL-6) and interleukin-1β (IL-1β) by RAW264.7 macrophage and the possible mechanism. Methods RAW264.7 cells were treated with 0.000, 0.125, 0.250, 0.500, 1.000, 2.000, 4.000 and 8.000 μg/mL rflaA to determine the EC50 of rflaA using CCK-8 assay. Secretion of IL-6 and IL-1β were measured by ELISA at 24, 36 and 48 hours after treatment of the cells with 0.04, 0.08 and 0.16 μg/mL rflaA...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28500222/energy-requirements-of-odor-transduction-in-the-chemosensory-cilia-of-olfactory-sensory-neurons-rely-on-oxidative-phosphorylation-and-glycolytic-processing-of-extracellular-glucose
#16
Pablo S Villar, Ricardo Delgado, Cecilia Vergara, Juan G Reyes, Juan Bacigalupo
The mechanisms that power the physiological events occuring in cilia, flagella and microvilli are of fundamental importance for the functions of these important and ubicuous organelles. The olfactory epithelium (OE) is mostly populated by ciliated olfactory sensory neurons (OSNs) and surrounding sustentacular cells (SCs) with apical microvilli. The only OSN dendrite extends to the surface forming a knob projecting several chemosensory cilia of approximately 50 x 0.2 μm, devoid of inner membranes embedded in a mucus layer...
May 12, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28495971/cilia-and-flagella-associated-protein-69-regulates-olfactory-transduction-kinetics-in-mice
#17
Anna K Talaga, Frederick N Dong, Johannes Reisert, Haiqing Zhao
Animals detect odorous chemicals through specialized olfactory sensory neurons (OSNs) that transduce odorants into neural electrical signals. We identify a novel and evolutionarily conserved protein, CFAP69, in mice that regulates olfactory transduction kinetics. In the olfactory epithelium CFAP69 is enriched in OSN cilia, where olfactory transduction occurs. Bioinformatic analysis suggests that a large portion of CFAP69 can form Armadillo-type α-helical repeats, which may mediate protein-protein interaction...
May 11, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28489911/human-%C3%AE-defensin-2-production-upon-viral-and-bacterial-co-infection-is-attenuated-in-copd
#18
Jason W Arnason, James C Murphy, Cora Kooi, Shahina Wiehler, Suzanne L Traves, Christopher Shelfoon, Barbara Maciejewski, Curtis J Dumonceaux, W Shawn Lewenza, David Proud, Richard Leigh
Viral-bacterial co-infections are associated with severe exacerbations of COPD. Epithelial antimicrobial peptides, including human β-defensin-2 (HBD-2), are integral to innate host defenses. In this study, we examined how co-infection of airway epithelial cells with rhinovirus and Pseudomonas aeruginosa modulates HBD-2 expression, and whether these responses are attenuated by cigarette smoke and in epithelial cells obtained by bronchial brushings from smokers with normal lung function or from COPD patients...
2017: PloS One
https://www.readbyqxmd.com/read/28489862/novel-genes-associated-with-enhanced-motility-of-escherichia-coli-st131
#19
Asha Kakkanat, Minh-Duy Phan, Alvin W Lo, Scott A Beatson, Mark A Schembri
Uropathogenic Escherichia coli (UPEC) is the cause of ~75% of all urinary tract infections (UTIs) and is increasingly associated with multidrug resistance. This includes UPEC strains from the recently emerged and globally disseminated sequence type 131 (ST131), which is now the dominant fluoroquinolone-resistant UPEC clone worldwide. Most ST131 strains are motile and produce H4-type flagella. Here, we applied a combination of saturated Tn5 mutagenesis and transposon directed insertion site sequencing (TraDIS) as a high throughput genetic screen and identified 30 genes associated with enhanced motility of the reference ST131 strain EC958...
2017: PloS One
https://www.readbyqxmd.com/read/28487689/cj0440c-affects-flagella-formation-and-in-vivo-colonization-of-erythromycin-susceptible-and-resistant-campylobacter-jejuni
#20
Haihong Hao, Xia Fang, Jing Han, Steven L Foley, Yulian Wang, Guyue Cheng, Xu Wang, Lingli Huang, Menghong Dai, Zhenli Liu, Zonghui Yuan
Campylobacter jejuni is one of the most common foodborne pathogen worldwide. A putative transcriptional regulator, Cj0440c, was up-regulated in the erythromycin-resistant C. jejuni, however, the precise role of Cj0440c is yet to be determined. The aim of this study was to determine the biological functions of Cj0440c. The Cj0440c isogenic mutants were constructed from erythromycin-susceptible C. jejuni NCTC 11168 (S) and -resistant C. jejuni 68-ER (R), designating as SM and RM, respectively. The isogenic Cj0440c mutants (SM and RM) and parental strains (S and R) were subjected to microarray and qRT-PCR analysis to examine the transcriptional profile changes contributed by Cj0440c...
2017: Frontiers in Microbiology
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