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https://www.readbyqxmd.com/read/28226241/catsper%C3%AE-regulates-the-structural-continuity-of-sperm-ca-2-signaling-domains-and-is-required-for-normal-fertility
#1
Jean-Ju Chung, Kiyoshi Miki, Doory Kim, Sang-Hee Shim, Huanan F Shi, Jae Yeon Hwang, Xinjiang Cai, Yusuf Iseri, Xiaowei Zhuang, David E Clapham
We report that the Gm7068 (CatSperε) and Tex40 (CatSperζ) genes encode novel subunits of a 9-subunit CatSper ion channel complex. Targeted disruption of CatSperζ reduces CatSper current and sperm rheotactic efficiency in mice, resulting in severe male subfertility. Normally distributed in linear quadrilateral nanodomains along the flagellum, the complex lacking CatSperζ is disrupted at ~0.8 μm intervals along the flagellum. This disruption renders the proximal flagellum inflexible and alters the 3D flagellar envelope, thus preventing sperm from reorienting against fluid flow in vitro and efficiently migrating in vivo...
February 22, 2017: ELife
https://www.readbyqxmd.com/read/28222296/bacterial-gliding-motility-rolling-out-a-consensus-model
#2
Beiyan Nan
Some bacteria glide mysteriously on surfaces without using flagella, pili, or other external appendages. Recent studies suggest how gliding motors in the inner membrane may transduce force to the cell surface.
February 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28217108/chemotaxis-without-conventional-two-component-system-based-on-cell-polarity-and-aerobic-conditions-in-helicity-switching-swimming-of-spiroplasma-eriocheiris
#3
Peng Liu, Huajun Zheng, Qingguo Meng, Natsuho Terahara, Wei Gu, Shengyue Wang, Guoping Zhao, Daisuke Nakane, Wen Wang, Makoto Miyata
Spiroplasma eriocheiris is a pathogen that causes mass mortality in Chinese mitten crab, Eriocheir sinensis. S. eriocheiris causes tremor disease and infects almost all of the artificial breeding crustaceans, resulting in disastrous effects on the aquaculture economy in China. S. eriocheiris is a wall-less helical bacterium, measuring 2.0 to 10.0 μm long, and can swim up to 5 μm per second in a viscous medium without flagella by switching the cell helicity at a kink traveling from the front to the tail. In this study, we showed that S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28210244/assessment-of-the-antimicrobial-activity-of-olive-leaf-extract-against-foodborne-bacterial-pathogens
#4
Yanhong Liu, Lindsay C McKeever, Nasir S A Malik
Olive leaf extract (OLE) has been used traditionally as a herbal supplement since it contains polyphenolic compounds with beneficial properties ranging from increasing energy levels, lowering blood pressure, and supporting the cardiovascular and immune systems. In addition to the beneficial effects on human health, OLE also has antimicrobial properties. The aim of this work was to investigate the antimicrobial effect of OLE against major foodborne pathogens, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella Enteritidis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28208560/re-patients-with-multiple-morphological-abnormalities-of-the-sperm-flagella-due-to-dnah1-mutations-have-a-good-prognosis-following-intracytoplasmic-sperm-injection
#5
https://www.readbyqxmd.com/read/28207750/functional-exploration-of-the-ift-a-complex-in-intraflagellar-transport-and-ciliogenesis
#6
Bing Zhu, Xin Zhu, Limei Wang, Yinwen Liang, Qianqian Feng, Junmin Pan
Intraflagellar transport (IFT) particles or trains are composed of IFT-A and IFT-B complexes. To assess the working mechanism of the IFT-A complex in IFT and ciliogenesis, we have analyzed ift43 mutants of Chlamydomnonas in conjunction with mutants of the other IFT-A subunits. An ift43 null mutant or a mutant with a partial deletion of the IFT43 conserved domain has no or short flagella. The mutants accumulate not only IFT-B but also IFT-Ain the short flagella, which is in contrast to an ift140 null mutant...
February 16, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28191473/evolution-of-the-immune-response-against-recombinant-proteins-tcpa-tcpb-and-flaa-as-a-candidate-subunit-cholera-vaccine
#7
Neda Molaee, Ghasem Mosayebi, Alireza Amozande-Nobaveh, Mohammad Reza Soleyman, Hamid Abtahi
Vibrio cholerae is the causative agent of cholera and annually leads to death of thousands of people around the globe. Two factors in the pathogenesis of this bacterium are its pili and flagella. The main subunits of pili TcpA, TcpB, and FlaA are the constituent subunit of flagella. In this study, we studied the ability of pili and flagella subunits to stimulate immune responses in mice. After amplification of TcpA, TcpB, and FlaA genes using PCR, they were cloned in expression plasmids. After production of the above-mentioned proteins by using IPTG, the proteins were purified and then approved using immunoblot method...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28180951/bradyrhizobium-centrolobii-and-bradyrhizobium-macuxiense-sp-nov-isolated-from-centrolobium-paraense-grown-in-soil-of-amazonia-brazil
#8
Daniele C Michel, Samuel R Passos, Jean L Simões-Araujo, Alexandre C Baraúna, Krisle da Silva, Marcia M Parma, Itamar S Melo, Sofie E De Meyer, Graham O'Hara, Jerri E Zilli
Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20-36 °C (optimal 28 °C), pH ranges 5-11 (optimal 6.0-7.0), and 0.1-0.5%NaCl (optimal 0.1-0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity...
February 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28177291/expression-of-flagellin-and-key-regulatory-flagellar-genes-in-the-non-motile-bacterium-piscirickettsia-salmonis
#9
Gabriela P Carril, Fernando A Gómez, Sergio H Marshall
The Piscirickettsia salmonis genome was screened to evaluate potential flagella-related open reading frames, as well as their genomic organization and eventual expression. A complete and organized set of flagellar genes was found for P. salmonis, although no structural flagellum has ever been reported for this bacterium. To gain further understanding, the hierarchical flagellar cascade described for Legionella pneumophila was used as a reference model for putative analysis in P. salmonis. Specifically, 5 of the most relevant genes from this cascade were chosen, including 3 regulatory genes (fleQ, triggers the cascade; fliA, regulates the σ28-coding gene; and rpoN, an RNA polymerase-dependent gene) and 2 terminal structural genes (flaA and flaB, flagellin and a flagellin-like protein, respectively)...
February 8, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28177288/novel-urease-negative-helicobacter-sp-h-enhydrae-sp-nov-isolated-from-inflamed-gastric-tissue-of-southern-sea-otters
#10
Zeli Shen, Francesca Batac, Anthony Mannion, Melissa A Miller, Vasudevan Bakthavatchalu, Calvin Ho, Sean Manning, Bruce J Paster, James G Fox
A total of 31 sea otters Enhydra lutris nereis found dead or moribund (and then euthanized) were necropsied in California, USA. Stomach biopsies were collected and transected with equal portions frozen or placed in formalin and analyzed histologically and screened for Helicobacter spp. in gastric tissue. Helicobacter spp. were isolated from 9 sea otters (29%); 58% (18 of 31) animals were positive for helicobacter by PCR. The Helicobacter sp. was catalase- and oxidase-positive and urease-negative. By electron microscopy, the Helicobacter sp...
February 8, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28176794/x-linked-primary-ciliary-dyskinesia-due-to-mutations-in-the-cytoplasmic-axonemal-dynein-assembly-factor-pih1d3
#11
Chiara Olcese, Mitali P Patel, Amelia Shoemark, Santeri Kiviluoto, Marie Legendre, Hywel J Williams, Cara K Vaughan, Jane Hayward, Alice Goldenberg, Richard D Emes, Mustafa M Munye, Laura Dyer, Thomas Cahill, Jeremy Bevillard, Corinne Gehrig, Michel Guipponi, Sandra Chantot, Philippe Duquesnoy, Lucie Thomas, Ludovic Jeanson, Bruno Copin, Aline Tamalet, Christel Thauvin-Robinet, Jean-François Papon, Antoine Garin, Isabelle Pin, Gabriella Vera, Paul Aurora, Mahmoud R Fassad, Lucy Jenkins, Christopher Boustred, Thomas Cullup, Mellisa Dixon, Alexandros Onoufriadis, Andrew Bush, Eddie M K Chung, Stylianos E Antonarakis, Michael R Loebinger, Robert Wilson, Miguel Armengot, Estelle Escudier, Claire Hogg, Serge Amselem, Zhaoxia Sun, Lucia Bartoloni, Jean-Louis Blouin, Hannah M Mitchison
By moving essential body fluids and molecules, motile cilia and flagella govern respiratory mucociliary clearance, laterality determination and the transport of gametes and cerebrospinal fluid. Primary ciliary dyskinesia (PCD) is an autosomal recessive disorder frequently caused by non-assembly of dynein arm motors into cilia and flagella axonemes. Before their import into cilia and flagella, multi-subunit axonemal dynein arms are thought to be stabilized and pre-assembled in the cytoplasm through a DNAAF2-DNAAF4-HSP90 complex akin to the HSP90 co-chaperone R2TP complex...
February 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28173168/mutational-analysis-and-overproduction-effects-of-motx-an-essential-component-for-motor-function-of-na-driven-polar-flagella-of-vibrio
#12
Norihiro Takekawa, Seiji Kojima, Michio Homma
No abstract text is available yet for this article.
February 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28164791/characterization-and-distinction-of-two-flagellar-systems-in-extraintestinal-pathogenic-escherichia-coli-pcn033
#13
Feng Liu, Jiyang Fu, Canying Liu, Jing Chen, Minhua Sun, Huanchun Chen, Chen Tan, Xiangru Wang
Extraintestinal pathogenic Escherichia coli (ExPEC) can invade and colonize multiple extraintestinal tissues and can cause a wide range of infections; however the mechanisms of its pathogenicity are not well understood. Flagella contribute to the infection of E. coli strains by mediating adhesion and invasion. Our previous bioinformatic analysis revealed two flagella gene clusters in the genome of an ExPEC isolate, PCN033. One encodes the conventional flagellum system (Flag-1) and the other encodes the Flag-2 system, whose function is uncharacterized...
March 2017: Microbiological Research
https://www.readbyqxmd.com/read/28152601/quantitative-proteomics-charts-the-landscape-of-salmonella-carbon-metabolism-within-host-epithelial-cells
#14
Yanhua Liu, Kaiwen Yu, Fan Zhou, Tao Ding, Yufei Yang, Mo Hu, Xiaoyun Liu
We performed a proteomic survey of Salmonella enterica serovar Typhimurium during infection of host epithelial cells. Our data reveal substantial metabolic reshuffling of Salmonella in the host in addition to severe degeneration of bacterial flagella and chemotaxis systems. The large-scale quantitative data allowed us to chart an overview of intracellular Salmonella carbon metabolism. Notably, we found preferential utilization of glycolysis, the pentose phosphate pathway, mixed acid fermentation, and nucleotide metabolism...
February 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28141484/parahaliea-aestuarii-sp-nov-isolated-from-the-asan-bay-estuary
#15
Hye Su Jung, Sang Eun Jeong, Kyung Hyun Kim, Che Ok Jeon
A Gram-stain-negative, strictly aerobic and moderate halotolerant bacterial strain, designated S2-26T, was isolated from the Asan Bay estuary in South Korea. Cells were motile rods with two polar flagella showing oxidase- and catalase-positive activities. Growth of strain S2-26T was observed at 15-45 C (optimum, 25C) and pH 5.5-10.0 (optimum, pH 7.0-8.5) and in the presence of 0-8.0 % (w/v) NaCl (optimum, 2.0 %). Strain S2-26T contained C17:1 ω8c, summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), summed feature 3 (comprising iso-C17:1 ω9c/C16:0 10-methyl) and C17:0 as the major fatty acids and ubiquinone-8 as the sole isoprenoid quinone...
January 29, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28139139/roseburia-spp-a-marker-of-health
#16
Zohreh Tamanai-Shacoori, Imen Smida, Latifa Bousarghin, Olivier Loreal, Vincent Meuric, Shao Bing Fong, Martine Bonnaure-Mallet, Anne Jolivet-Gougeon
The genus Roseburia consists of obligate Gram-positive anaerobic bacteria that are slightly curved, rod-shaped and motile by means of multiple subterminal flagella. It includes five species: Roseburia intestinalis, R. hominis, R. inulinivorans, R. faecis and R. cecicola. Gut Roseburia spp. metabolize dietary components that stimulate their proliferation and metabolic activities. They are part of commensal bacteria producing short-chain fatty acids, especially butyrate, affecting colonic motility, immunity maintenance and anti-inflammatory properties...
February 2017: Future Microbiology
https://www.readbyqxmd.com/read/28138098/genetic-and-mechanistic-analyses-of-the-periplasmic-domain-of-the-enterohemorrhagic-e-coli-ehec-qsec-histidine-sensor-kinase
#17
Christopher T Parker, Regan Russell, Jacqueline W Njoroge, Angel G Jimenez, Ron Taussig, Vanessa Sperandio
: The histidine sensor kinase (HK) QseC, senses autoinducer-3 (AI-3), and the adrenergic hormones epinephrine and norepinephrine. Upon sensing these signals, QseC acts through three response regulators (RRs) to regulate expression of virulence genes in enterohemorrhagic E. coli (EHEC). The QseB, QseF and KdpE RRs that are phosphorylated by QseC constitute a tripartite signaling cascade having different and overlapping targets, including flagella and motility, the type three secretion system encoded by the locus of enterocyte effacement (LEE), and Shiga toxin...
January 30, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28137312/transcriptional-regulation-of-human-sperm-associated-antigen-16-gene-by-s-sox5
#18
Ling Zhang, Yunhao Liu, Wei Li, Qiaoling Zhang, Yanwei Li, Junpin Liu, Jie Min, Chaofan Shuang, Shizheng Song, Zhibing Zhang
BACKGROUND: The mammalian sperm-associated antigen 16 gene (Spag16) uses alternative promoters to produce two major transcript isoforms (Spag16L and Spag16S) and encode proteins that are involved in the cilia/flagella formation and motility. In silico analysis of both mouse and human SPAG16L promoters reveals the existence of multiple putative SOX5 binding sites. Given that the SOX5 gene encodes a 48-kDa transcription factor (S-SOX5) and the presence of putative SOX5 binding sites at the SPAG16L promoter, regulation of SPAG16L expression by S-SOX5 was studied in the present work...
January 31, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28133926/n-acylhomoserine-lactone-regulation-of-genes-mediating-motility-and-pathogenicity-in-pseudomonas-syringae-pathovar-tabaci-11528
#19
Feifei Cheng, Anzhou Ma, Jinxue Luo, Xuliang Zhuang, Guoqiang Zhuang
Pseudomonas syringae pathovar tabaci 11528 (P. syringae 11528) is a phytopathogen that causes wild-fire disease in soybean and tobacco plants. It utilizes a cell density-dependent regulation system known as quorum sensing (QS). In its QS system, the psyI is responsible for the biosynthesis of N-acylhomoserine lactones (AHLs). By comparing the transcripts from P. syringae 11528 wild-type strain with those of the ΔpsyI mutant using RNA sequencing (RNA-seq) technology, 1118 AHL-regulated genes were identified in the transition from exponential to stationary growth phase...
January 29, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28131374/salmonella-enteritidis-flagellar-mutants-have-a-colonization-benefit-in-the-chicken-oviduct
#20
Sofie Kilroy, Ruth Raspoet, An Martel, Leslie Bosseler, Corinne Appia-Ayme, Arthur Thompson, Freddy Haesebrouck, Richard Ducatelle, Filip Van Immerseel
Egg borne Salmonella Enteritidis is still a major cause of human food poisoning. Eggs can become internally contaminated following colonization of the hen's oviduct. In this paper we aimed to analyze the role of flagella of Salmonella Enteritidis in colonization of the hen's oviduct. Using a transposon library screen we showed that mutants lacking functional flagella are significantly more efficient in colonizing the hen's oviduct in vivo. A micro-array analysis proved that transcription of a number of flagellar genes is down-regulated inside chicken oviduct cells...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
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