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https://www.readbyqxmd.com/read/28636910/prp40-homolog-a-is-a-novel-centrin-target
#1
Adalberto Díaz Casas, Walter J Chazin, Belinda Pastrana-Ríos
Pre-mRNA processing protein 40 (Prp40) is a nuclear protein that has a role in pre-mRNA splicing. Prp40 possesses two leucine-rich nuclear export signals, but little is known about the function of Prp40 in the export process. Another protein that has a role in protein export is centrin, a member of the EF-hand superfamily of Ca(2+)-binding proteins. Prp40 was found to be a centrin target by yeast-two-hybrid screening using both Homo sapiens centrin 2 (Hscen2) and Chlamydomonas reinhardtii centrin (Crcen). We identified a centrin-binding site within H...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28634124/cell-disruption-and-lipid-extraction-for-microalgal-biorefineries-a-review
#2
REVIEW
Soo Youn Lee, Jun Muk Cho, Yong Keun Chang, You-Kwan Oh
The microalgae-based biorefinement process has attracted much attention from academic and industrial researchers attracted to its biofuel, food and nutraceutical applications. In this paper, recent developments in cell-disruption and lipid-extraction methods, focusing on four biotechnologically important microalgal species (namely, Chlamydomonas, Haematococcus, Chlorella, and Nannochloropsis spp.), are reviewed. The structural diversity and rigidity of microalgal cell walls complicate the development of efficient downstream processing methods for cell-disruption and subsequent recovery of intracellular lipid and pigment components...
June 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28633328/identification-and-characterization-of-a-solute-carrier-cia8-involved-in-inorganic-carbon-acclimation-in-chlamydomonas-reinhardtii
#3
Marylou C Machingura, Joanna Bajsa-Hirschel, Susan M Laborde, Joshua B Schwartzenburg, Bratati Mukherjee, Ananya Mukherjee, Steve V Pollock, Britta Förster, G Dean Price, James V Moroney
The supply of inorganic carbon (Ci) at the site of fixation by Rubisco is a key parameter for efficient CO2 fixation in aquatic organisms including the green alga, Chlamydomonas reinhardtii. Chlamydomonas reinhardtii cells, when grown on limiting CO2, have a CO2-concentrating mechanism (CCM) that functions to concentrate CO2 at the site of Rubisco. Proteins thought to be involved in inorganic carbon uptake have been identified and localized to the plasma membrane or chloroplast envelope. However, current CCM models suggest that additional molecular components are involved in Ci uptake...
June 13, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28630812/molecular-properties-of-a-dtd-channelrhodopsin-from-guillardia-theta
#4
Yumeka Yamauchi, Masae Konno, Shota Ito, Satoshi P Tsunoda, Keiichi Inoue, Hideki Kandori
Microbial rhodopsins are membrane proteins found widely in archaea, eubacteria and eukaryotes (fungal and algal species). They have various functions, such as light-driven ion pumps, light-gated ion channels, light sensors and light-activated enzymes. A light-driven proton pump bacteriorhodopsin (BR) contains a DTD motif at positions 85, 89, and 96, which is unique to archaeal proton pumps. Recently, channelrhodopsins (ChRs) containing the DTD motif, whose sequential identity is ~20% similar to BR and to cation ChRs in Chlamydomonas reinhardtii (CrCCRs), were found...
2017: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/28630648/comparative-metabolic-profiling-of-the-lipid-producing-green-microalga-chlorella-reveals-that-nitrogen-and-carbon-metabolic-pathways-contribute-to-lipid-metabolism
#5
Hui Chen, Yanli Zheng, Jiao Zhan, Chenliu He, Qiang Wang
Microalgae are a promising feedstock for biofuel production. Microalgal metabolic pathways are heavily influenced by environmental factors. For instance, lipid metabolism can be induced by nitrogen-limiting conditions. However, the underlying mechanisms of lipid biosynthesis are unclear. In this study, we analyzed the global metabolic profiles of three genetically closely related Chlorella strains (C1, C2, and C3) with significant differences in lipid productivity to identify the contributions of key metabolic pathways to lipid metabolism...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28630322/tcte1-is-a-conserved-component-of-the-dynein-regulatory-complex-and-is-required-for-motility-and-metabolism-in-mouse-spermatozoa
#6
Julio M Castaneda, Rong Hua, Haruhiko Miyata, Asami Oji, Yueshuai Guo, Yiwei Cheng, Tao Zhou, Xuejiang Guo, Yiqiang Cui, Bin Shen, Zibin Wang, Zhibin Hu, Zuomin Zhou, Jiahao Sha, Renata Prunskaite-Hyyrylainen, Zhifeng Yu, Ramiro Ramirez-Solis, Masahito Ikawa, Martin M Matzuk, Mingxi Liu
Flagella and cilia are critical cellular organelles that provide a means for cells to sense and progress through their environment. The central component of flagella and cilia is the axoneme, which comprises the "9+2" microtubule arrangement, dynein arms, radial spokes, and the nexin-dynein regulatory complex (N-DRC). Failure to properly assemble components of the axoneme leads to defective flagella and in humans leads to a collection of diseases referred to as ciliopathies. Ciliopathies can manifest as severe syndromic diseases that affect lung and kidney function, central nervous system development, bone formation, visceral organ organization, and reproduction...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628990/kinetic-modelling-of-starch-and-lipid-formation-during-mixotrophic-nutrient-limited-microalgal-growth
#7
Gonzalo M Figueroa-Torres, Jon K Pittman, Constantinos Theodoropoulos
Microalgal starch and lipids, carbon-based storage molecules, are useful as potential biofuel feedstocks. In this work, cultivation strategies maximising starch and lipid formation were established by developing a multi-parameter kinetic model describing microalgal growth as well as starch and lipid formation, in conjunction with laboratory-scale experiments. Growth dynamics are driven by nitrogen-limited mixotrophic conditions, known to increase cellular starch and lipid contents whilst enhancing biomass growth...
June 2, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28624997/expression-and-purification-of-cytochrome-p450-55b1-from-chlamydomonas-reinhardtii-and-its-application-in-nitric-oxide-biosensing
#8
Bin Gong, Xiaosheng Liang, Yong Li, Qian Xiao, Panchun Yang, Yunhua Wu
Cytochrome P450 55B1 from Chlamydomonas reinhardtii is reported to function as a nitric oxide reductase (NOR). Here, we expressed the cytochrome P450 55B1 gene with an HIS-tag in E scherichia coli using a pET28a vector. The native protein was produced at a level of 1.59 μmol/g of total protein, with approximately 85% of the P450 being soluble. The CYP55B1 protein was characterized spectrally and purified by a HIS-trap column. This procedure allowed recovery of 45% of the expressed protein and CYP55B1 with a specific content of 0...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28624244/evolutionary-engineering-of-salt-resistant-chlamydomonas-sp-strains-reveals-salinity-stress-activated-starch-to-lipid-biosynthesis-switching
#9
Yuichi Kato, Shih-Hsin Ho, Christopher J Vavricka, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo
The aim of this study was to improve biomass production of the green microalga Chlamydomonas sp. JSC4 under high salinity conditions. For this purpose, heavy ion beam-coupled mutagenesis and evolutionary engineering were performed using JSC4 as the parent strain. After long-term and continuous cultivation with high salinity, salt-resistant strains that grow well even in the presence of 7% sea salt were successfully obtained. Transcriptional analysis revealed inactivation of starch-to-lipid biosynthesis switching, which resulted in delayed starch degradation and decreased lipid content in the salt-resistant strains...
June 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28620699/evolution-of-chlamydomonas-reinhardtii-ferredoxins-and-their-interactions-with-fefe-hydrogenases
#10
REVIEW
Anne Sawyer, Martin Winkler
Ferredoxins are soluble iron sulphur proteins which function as electron donors in a number of metabolic pathways in a broad range of organisms. In photosynthetic organisms, PETF, or ferredoxin 1 (FDX1), is the most studied ferredoxin due to its essential role in photosynthesis, where it transfers electrons from photosystem I to ferredoxin-NADP(+) oxidoreductase. However, PETF can also transfer electrons to a large number of other proteins. One important PETF electron acceptor found in green microalgae is the biologically and biotechnologically important [FeFe]-hydrogenase HYDA, which catalyses the production of molecular hydrogen (H2) from protons and electrons...
June 15, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28619534/cryptochrome-photoreceptors-in-green-algae-unexpected-versatility-of-mechanisms-and-functions
#11
Tilman Kottke, Sabine Oldemeyer, Sandra Wenzel, Yong Zou, Maria Mittag
Green algae have a highly complex and diverse set of cryptochrome photoreceptor candidates including members of the following subfamilies: plant, plant-like, animal-like, DASH and cryptochrome photolyase family 1 (CPF1). While some green algae encode most or all of them, others lack certain members. Here we present an overview about functional analyses of so far investigated cryptochrome photoreceptors from the green algae Chlamydomonas reinhardtii (plant and animal-like cryptochromes) and Ostreococcus tauri (CPF1) with regard to their biological significance and spectroscopic properties...
May 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28618080/electrocatalytic-mechanism-involving-michaelis-menten-kinetics-at-the-preparative-scale-theory-and-applicability-to-photocurrents-from-photosynthetic-algae-suspension-with-quinones
#12
Guillaume Longatte, Manon Guille-Collignon, Frédéric Lemaître
In the past years, many strategies were implemented to take benefits from oxygenic photosynthesis to harvest photosynthetic electrons and produce a significant photocurrent. Therefore, electrochemical tools were considered and globally relied on the electron transfer(s) between the photosynthetic chain and a collecting electrode. In this context, we recently reported the implementation of an electrochemical set-up at the preparative scale to produce photocurrents from a Chlamydomonas reinhardtii algae suspension with an appropriate mediator (2,6-DCBQ) and a carbon gauze as the working electrode...
June 15, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28615673/phototaxis-beyond-turning-persistent-accumulation-and-response-acclimation-of-the-microalga-chlamydomonas-reinhardtii
#13
Jorge Arrieta, Ana Barreira, Maurizio Chioccioli, Marco Polin, Idan Tuval
Phototaxis is an important reaction to light displayed by a wide range of motile microorganisms. Flagellated eukaryotic microalgae in particular, like the model organism Chlamydomonas reinhardtii, steer either towards or away from light by a rapid and precisely timed modulation of their flagellar activity. Cell steering, however, is only the beginning of a much longer process which ultimately allows cells to determine their light exposure history. This process is not well understood. Here we present a first quantitative study of the long timescale phototactic motility of Chlamydomonas at both single cell and population levels...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28612271/overexpression-of-dnaj-like-chaperone-enhances-carotenoid-synthesis-in-chlamydomonas-reinhardtii
#14
Tateki Morikawa, Yusuke Uraguchi, Shohei Sanda, Satoshi Nakagawa, Shigeki Sawayama
Production of functional carotenoids using microalgae may facilitate the commercialization of anti-aging nutritional supplements. The green alga Chlamydomonas reinhardtii uses a non-mevalonate (MEP) pathway for isopentenyl diphosphate (IPP) synthesis. Two enzymes thought to play important roles in this MEP pathway to IPP synthesis are 1-deoxy-D-xylulose 5-phosphate synthase (DXS) and reductase (DXR). DnaJ-like chaperone (Orange protein) is thought to support phytoene synthase, a key enzyme in plant carotenoid synthesis...
June 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28608341/isolation-and-characterization-of-argonaute-mutants-in-chlamydomonas
#15
Tomohito Yamasaki
Random insertional mutagenesis and subsequent reverse genetic screening allow the isolation of mutants of interest. Here I describe the protocol for generating a tag insertion line and subsequent PCR-based screening for ARGONAUTE mutants as an example of a reverse genetic screen for the unicellular green alga Chlamydomonas reinhardtii.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28598481/pools-of-cadmium-in-chlamydomonas-reinhardtii-revealed-by-chemical-imaging-and-xas-spectroscopy
#16
F Penen, M P Isaure, D Dobritzsch, I Bertalan, H Castillo-Michel, O Proux, E Gontier, P Le Coustumer, D Schaumlöffel
The green micro-alga Chlamydomonas reinhardtii is commonly used as a model to investigate metallic stress in photosynthetic organisms. The aim of this study was to explore processes implemented by three C. reinhardtii strains to cope with cadmium (Cd), and particularly to evidence Cd sequestration in the cell. For that, we used a combination of subcellular fractionation and chemical imaging (micro X-ray fluorescence (μXRF) and transmission electron microscopy (TEM/X-EDS)) to identify subcellular compartments of Cd accumulation, and X-ray absorption spectroscopy (XAS) to determine chemical Cd speciation...
June 9, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28597046/molecular-cloning-and-expression-analysis-of-major-intrinsic-protein-gene-in-chlamydomonas-sp-ice-l-from-antarctica
#17
Lulu Li, Meiling An, Changfeng Qu, Zhou Zheng, Yibin Wang, Fangming Liu, Yingying He, Xiaodong He, Jinlai Miao
Major intrinsic proteins (MIPs) form channels facilitating the passive transport of water and other small polar molecules across membranes. In this study, the complete open reading frame (ORF) of CiMIP1 (GenBank ID KY316061) encoding one kind of MIPs in the Antarctic ice microalga Chlamydomonas sp. ICE-L is successfully cloned using RACE. In addition, the expression patterns of CiMIP1 gene under different conditions of temperature and salinity are determined by qRT-PCR. The ORF of CiMIP1 gene encodes 308 amino acids, and the deduced amino acid sequence shows 74% homology with Chlamydomonas reinhardtii CrMIP1 (GenBank number 159471952)...
July 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28596334/self-repairing-cells-how-single-cells-heal-membrane-ruptures-and-restore-lost-structures
#18
REVIEW
Sindy K Y Tang, Wallace F Marshall
Many organisms and tissues display the ability to heal and regenerate as needed for normal physiology and as a result of pathogenesis. However, these repair activities can also be observed at the single-cell level. The physical and molecular mechanisms by which a cell can heal membrane ruptures and rebuild damaged or missing cellular structures remain poorly understood. This Review presents current understanding in wound healing and regeneration as two distinct aspects of cellular self-repair by examining a few model organisms that have displayed robust repair capacity, including Xenopus oocytes, Chlamydomonas, and Stentor coeruleus Although many open questions remain, elucidating how cells repair themselves is important for our mechanistic understanding of cell biology...
June 9, 2017: Science
https://www.readbyqxmd.com/read/28593495/association-of-ferredoxin-nadp-oxidoreductase-with-the-photosynthetic-apparatus-modulates-electron-transfer-in-chlamydomonas-reinhardtii
#19
Laura Mosebach, Claudia Heilmann, Risa Mutoh, Philipp Gäbelein, Janina Steinbeck, Thomas Happe, Takahisa Ikegami, Guy Hanke, Genji Kurisu, Michael Hippler
Ferredoxins (FDX) and the FDX:NADP(+) oxidoreductase (FNR) represent a key junction of electron transport downstream of photosystem I (PSI). Dynamic recruitment of FNR to the thylakoid membrane has been considered as a potential mechanism to define the fate of photosynthetically derived electrons. In this study, we investigated the functional importance of the association of FNR with the photosynthetic apparatus in Chlamydomonas reinhardtii. In vitro assays based on NADP(+) photoreduction measurements as well as NMR chemical shift perturbation analyses showed that FNR preferentially interacts with FDX1 compared to FDX2...
June 7, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28588557/the-mechanism-of-starch-over-accumulation-in-chlamydomonas-reinhardtii-high-starch-mutants-identified-by-comparative-transcriptome-analysis
#20
Kwang M Koo, Sera Jung, Beom S Lee, Jin-Baek Kim, Yeong D Jo, Hong-Il Choi, Si-Yong Kang, Gook-H Chung, Won-Joong Jeong, Joon-Woo Ahn
The focus of this study was the mechanism of starch accumulation in Chlamydomonas reinhardtii high-starch mutants. Three C. reinhardtii mutants showing high-starch content were generated using gamma irradiation. When grown under nitrogen-deficient conditions, these mutants had more than twice as much starch than a wild-type control. The mechanism of starch over-accumulation in these mutants was studied with comparative transcriptome analysis. In all mutants, induction of phosphoglucomutase 1 (PGM1) expression was detected; PGM1 catalyzes the inter-conversion of glucose 1-phosphate and glucose 6-phosphate in both starch biosynthetic and glycolytic pathway...
2017: Frontiers in Microbiology
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