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https://www.readbyqxmd.com/read/28432498/co-cultivation-of-chlamydomonas-reinhardtii-with-azotobacter-chroococcum-improved-h2-production
#1
Lili Xu, Xianglong Cheng, Shuangxiu Wu, Quanxi Wang
OBJECTIVES: To improve H2 production, the green algae Chlamydomonas reinhardtii cc849 was co-cultured with Azotobacter chroococcum. RESULTS: The maximum H2 production of the co-culture was 350% greater than that of the pure algal cultures under optimal H2 production conditions. The maximum growth and the respiratory rate of the co-cultures were about 320 and 300% of the controls, and the dissolved O2 of co-cultures was decreased 74%. Furthermore, the in vitro maximum hydrogenase activity of the co-culture was 250% greater than that of the control, and the in vivo maximum hydrogenase activity of the co-culture was 1...
April 21, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28428259/rabl2-interacts-with-the-ift-b-complex-and-cep19-and-participates-in-ciliary-assembly
#2
Yuya Nishijima, Yohei Hagiya, Tomohiro Kubo, Ryota Takei, Yohei Katoh, Kazuhisa Nakayama
Proteins localized to the basal body and the centrosome play crucial roles in ciliary assembly and functions. Although RABL2 and CEP19 are conserved in ciliated organisms and have been implicated in ciliary/flagellar functions, their roles were poorly understood. We here showed that RABL2 interacts with CEP19 and is recruited to the mother centriole and basal body in a CEP19-dependent manner, and that CEP19 is recruited to the centriole probably via its binding to the centrosomal protein FGFR1OP. Disruption of the RABL2 gene in Chlamydomonas reinhardtii resulted in the non-flagellated phenotype, suggesting a crucial role of RABL2 in ciliary/flagellar assembly...
April 20, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28424932/inverse-relationship-of-ca-2-dependent-flagellar-response-between-animal-sperm-and-prasinophyte-algae
#3
Kogiku Shiba, Kazuo Inaba
Symmetry/asymmetry conversion of eukaryotic flagellar waveform is caused by the changes in intracellular Ca(2+). Animal sperm flagella show symmetric or asymmetric waveform at lower or higher concentration of intracellular Ca(2+), respectively. In Chlamydomonas, high Ca(2+) induces conversion of flagellar waveform from asymmetric to symmetry, resulting in the backward movement. This mirror image relationship between animal sperm and Chlamydomonas could be explained by the distinct calcium sensors used to regulate the outer arm dyneins (Inaba 2015)...
April 19, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28421378/transcriptome-based-identification-of-the-desiccation-response-genes-in-marine-red-algae-pyropia-tenera-rhodophyta-and-enhancement-of-abiotic-stress-tolerance-by-ptdrg2-in-chlamydomonas
#4
Sungoh Im, Ha-Nul Lee, Hyun Shin Jung, Sunghwan Yang, Eun-Jeong Park, Mi Sook Hwang, Won-Joong Jeong, Dong-Woog Choi
Pyropia tenera (Kjellman) are marine red algae that grow in the intertidal zone and lose more than 90% of water during hibernal low tides every day. In order to identify the desiccation response gene (DRG) in P. tenera, we generated 1,444,210 transcriptome sequences using the 454-FLX platform from the gametophyte under control and desiccation conditions. De novo assembly of the transcriptome reads generated 13,170 contigs, covering about 12 Mbp. We selected 1160 differentially expressed genes (DEGs) in response to desiccation stress based on reads per kilobase per million reads (RPKM) expression values...
April 18, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28417161/ift54-regulates-ift20-stability-but-is-not-essential-for-tubulin-transport-during-ciliogenesis
#5
Xin Zhu, Yinwen Liang, Feng Gao, Junmin Pan
Intraflagellar transport (IFT) is required for ciliogenesis by ferrying ciliary components using IFT complexes as cargo adaptors. IFT54 is a component of the IFT-B complex and is also associated with cytoplasmic microtubules (MTs). Loss of IFT54 impairs cilia assembly as well as cytoplasmic MT dynamics. The N-terminal calponin homology (CH) domain of IFT54 interacts with tubulins/MTs and has been proposed to transport tubulin during ciliogenesis, whereas the C-terminal coiled-coil (CC) domain binds IFT20. However, the precise function of these domains in vivo is not well understood...
April 17, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28415213/non-gaussian-limit-fluctuations-in-active-swimmer-suspensions
#6
Takashi Kurihara, Msato Aridome, Heev Ayade, Irwin Zaid, Daisuke Mizuno
We investigate the hydrodynamic fluctuations in suspensions of swimming microorganisms (Chlamydomonas) by observing the probe particles dispersed in the media. Short-term fluctuations of probe particles were superdiffusive and displayed heavily tailed non-Gaussian distributions. The analytical theory that explains the observed distribution was derived by summing the power-law-decaying hydrodynamic interactions from spatially distributed field sources (here, swimming microorganisms). The summing procedure, which we refer to as the physical limit operation, is applicable to a variety of physical fluctuations to which the classical central limiting theory does not apply...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28413446/artificial-mirna-inhibition-of-phosphoenolpyruvate-carboxylase-increases-fatty-acid-production-in-a-green-microalga-chlamydomonas-reinhardtii
#7
Chaogang Wang, Xi Chen, Hui Li, Jiangxin Wang, Zhangli Hu
BACKGROUND: Nutrient limitation, such as nitrogen depletion, is the most widely used method for improving microalgae fatty acid production; however, these harsh conditions also inhibit algal growth significantly and even kill cells at all. To avoid these problems, we used artificial microRNA (amiRNA) technology as a useful tool to manipulate metabolic pathways to increase fatty acid contents effectively in the green microalga Chlamydomonas reinhardtii. We down-regulated the expression of phosphoenolpyruvate carboxylase (PEPC), which catalyzes the formation of oxaloacetate from phosphoenolpyruvate and regulates carbon flux...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28413444/functional-photosystem-i-maintains-proper-energy-balance-during-nitrogen-depletion-in-chlamydomonas-reinhardtii-promoting-triacylglycerol-accumulation
#8
Mahmoud Gargouri, Philip D Bates, Jeong-Jin Park, Helmut Kirchhoff, David R Gang
BACKGROUND: Nutrient deprivation causes significant stress to the unicellular microalga, Chlamydomonas reinhardtii, which responds by significantly altering its metabolic program. Following N deprivation, the accumulation of starch and triacylglycerols (TAGs) is significantly altered following massive reprogramming of cellular metabolism. One protein that was found to change dramatically and early to this stress was TAB2, a photosystem I (PSI) translation initiation factor, whose transcript and protein levels increased significantly after only 30 min of N deprivation...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28411583/how-plants-cope-with-uv-b-from-perception-to-response
#9
REVIEW
Ruohe Yin, Roman Ulm
Ultraviolet-B radiation (UV-B) is an intrinsic part of the solar radiation that reaches the Earth's surface and affects the biosphere. Plants have evolved a specific UV-B signaling pathway mediated by the UVR8 photoreceptor that regulates growth, development, and acclimation. Major recent advances have contributed to our understanding of the UVR8 photocycle, UV-B-responsive protein-protein interactions, regulation of UVR8 subcellular localization, and UVR8-regulated physiological responses. Here, we review the latest progress in our understanding of UVR8 signaling and UV-B responses, which includes studies in the unicellular alga Chlamydomonas reinhardtii and the flowering plant Arabidopsis...
April 12, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28409092/the-search-for-a-lipid-trigger-the-effect-of-salt-stress-on-the-lipid-profile-of-the-model-microalgal-species-chlamydomonas-reinhardtii-for-biofuels-production
#10
Emily Hounslow, Rahul Vijay Kapoore, Seetharaman Vaidyanathan, D James Gilmour, Phillip C Wright
BACKGROUND: Algal cells produce neutral lipid when stressed and this can be used to generate biodiesel. OBJECTIVE: Salt stressed cells of the model microalgal species Chlamydomonas reinhardtii were tested for their suitability to produce lipid for biodiesel. METHODS: The starchless mutant of C. reinhardtii (CC-4325) was subjected to salt stress (0.1, 0.2 and 0.3 M NaCl) and transesterification and GC analysis were used to determine fatty acid methyl ester (FAME) content and profile...
November 2016: Current Biotechnology
https://www.readbyqxmd.com/read/28401332/a-method-for-microalgae-proteomics-analysis-based-on-modified-filter-aided-sample-preparation
#11
Song Li, Xupeng Cao, Yan Wang, Zhen Zhu, Haowei Zhang, Song Xue, Jing Tian
With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports...
April 11, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28392829/a-robust-protocol-for-efficient-generation-and-genomic-characterization-of-insertional-mutants-of-chlamydomonas-reinhardtii
#12
Steve V Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C Machingura, Ananya Mukherjee, Arthur R Grossman, James V Moroney
BACKGROUND: Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants have helped elucidate the genetic basis of various physiological processes in this model organism. Unfortunately, the insertion sites of many interesting mutants are never defined due to experimental difficulties in establishing the location of the inserted cassette in the Chlamydomonas genome...
2017: Plant Methods
https://www.readbyqxmd.com/read/28386962/comparative-analyses-of-h2-photoproduction-in-magnesium-and-sulfur-starved-chlamydomonas-reinhardtii-cultures
#13
Alena A Volgusheva, Martina Jokel, Yagut Allahverdiyeva, Galina P Kukarskikh, Eugeni P Lukashev, Maya D Lambreva, Tatayana E Krendeleva, Taras K Antal
Magnesium (Mg)-deprived Chlamydomonas reinhardtii cells are capable to sustain hydrogen (H2 ) photoproduction at relatively high PSII activity levels for an extended time period as compared with sulfur (S)-deprived cells. Herein, we present a comparative study of H2 photoproduction induced by Mg and S shortage to unravel the specific rearrangements of photosynthetic machinery and cell metabolism occurring under the two deprivation protocols. The exhaustive analysis of photosynthetic activity and regulatory pathways, respiration, and starch metabolism revealed the specific rearrangements of photosynthetic machinery and cellular metabolism, which occur under the two deprivation conditions...
April 7, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28378041/precise-oligonucleotide-directed-mutagenesis-of-the-chlamydomonas-reinhardtii-genome
#14
Wen-Zhi Jiang, Sarah Dumm, Mark E Knuth, Steven L Sanders, Donald P Weeks
No abstract text is available yet for this article.
April 4, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28377049/the-endocytic-pathways-of-a-secretory-granule-membrane-protein-in-hek293-cells-pam-and-egf-traverse-a-dynamic-multivesicular-body-network-together
#15
Nils Bäck, Kristiina Kanerva, Vishwanatha Kurutihalli, Andrew Yanik, Elina Ikonen, Richard E Mains, Betty A Eipper
Peptidylglycine α-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs)...
March 28, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28375577/electrokinetically-controlled-fluid-injection-into-unicellular-microalgae
#16
Xuewen Zhou, Xixi Zhang, Jonathan Boualavong, Andrew R Durney, Tonghui Wang, Scott Kirschner, Michaela Wentz, Hitomi Mukaibo
Electrokinetically-controlled microinjection is reported as an effective transport mechanism for microinjection into the wild-type strain of the widely-studied model microalga Chlamydomonas reinhardtii. Microinjection system using glass capillary pipettes was developed to capture and impale the motile cell. To apply an electric field and induce electrokinetic flow (e.g. electrophoresis and electroosmosis), an electrode was inserted directly to the solution inside the impaling injection pipette (IP) and another electrode was inserted into the external cell media...
April 4, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28374798/dynamic-metabolic-profiling-together-with-transcription-analysis-reveals-salinity-induced-starch-to-lipid-biosynthesis-in-alga-chlamydomonas-sp-jsc4
#17
Shih-Hsin Ho, Akihito Nakanishi, Yuichi Kato, Hiroaki Yamasaki, Jo-Shu Chang, Naomi Misawa, Yuu Hirose, Jun Minagawa, Tomohisa Hasunuma, Akihiko Kondo
Biodiesel production using microalgae would play a pivotal role in satisfying future global energy demands. Understanding of lipid metabolism in microalgae is important to isolate oleaginous strain capable of overproducing lipids. It has been reported that reducing starch biosynthesis can enhance lipid accumulation. However, the metabolic mechanism controlling carbon partitioning from starch to lipids in microalgae remains unclear, thus complicating the genetic engineering of algal strains. We here used "dynamic" metabolic profiling and essential transcription analysis of the oleaginous green alga Chlamydomonas sp...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28372038/highly-time-resolved-metabolic-reprogramming-toward-differential-levels-of-phosphate-in-chlamydomonas-reinhardtii
#18
Cheol-Ho Jang, Gayeon Lee, Yong-Cheol Park, Kyoung Heon Kim, Do Yup Lee
Understanding phosphorus metabolism in photosynthetic organisms is important in that it is closely associated with enhanced crop productivity and pollution management for natural ecosystems (e.g. algal blooming). Accordingly, we exploited highly time-resolved metabolic responses to different levels of phosphate deprivation in C. reinhardtii, a photosynthetic model organism. We conducted non-targeted primary metabolite profiling using gas-chromatography time-of-flight mass spectrometric analysis. Primarily, we systematically identified main contributors to degree-wise responses corresponding to the deprivation levels of phosphate...
April 3, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28371711/direct-and-indirect-effects-of-silver-nanoparticles-on-freshwater-and-marine-microalgae-chlamydomonas-reinhardtii-and-phaeodactylum-tricornutum
#19
M Sendra, M P Yeste, J M Gatica, I Moreno-Garrido, J Blasco
The last decade has seen a considerable increase in the use of silver nanoparticles (AgNPs), which are found in many every-day consumer products including textiles, plastics, cosmetics, household sprays and paints. The release of those AgNPs into aquatic environments could be causing ecological damage. In this study we assess the toxicity of AgNPs of different sizes to two species of microalgae, from freshwater and marine environment (Chlamydomonas reinhardtii and Phaeodactylum tricornutum respectively). Dissolution processes affect the form and concentration of AgNPs in both environments...
March 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/28364782/the-g-protein-alpha-subunit-gene-cga1-is-involved-in-regulation-of-resistance-to-heat-and-osmotic-stress-in-chlamydomonas-reinhardtii
#20
C S Lee, W Ahn, Y E Choi
In eukaryotic cells, many important functions of specific G-proteins have been identified, but microalgal G-proteins are poorly studied. In this work, we characterized a gene (CGA1) encoding the G-protein α-subunit in Chlamydomonas reinhardtii. Independent knockdown mutants of CGA1 were generated via RNA interference (RNAi). CGA1 expression levels were consistently and significantly reduced in both independent CGA1 mutant cell lines (cga1). Both cga1 mutants had a higher survival rate at 35°C in comparison with the wild type...
February 28, 2017: Cellular and Molecular Biology
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