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Cytochrome c oxydase

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https://www.readbyqxmd.com/read/27470945/the-increase-in-fat-content-in-the-warm-acclimated-striped-hamsters-is-associated-with-the-down-regulated-metabolic-thermogenesis
#1
Song Tan, Jing Wen, Lu-Lu Shi, Chun-Ming Wang, Gui-Ying Wang, Zhi-Jun Zhao
It has been well known that metabolic thermogenesis plays an important role in the thermoregulation of small mammals under different temperatures, while its role in fat accumulation is far from clear. In the present study, several physiological, hormonal, and biochemical measures indicative of metabolic thermogenesis were measured in the weaning striped hamsters after acclimated to a warm condition (30°C) for 1, 3 and 4months. The warm-acclimated groups significantly decreased energy intake, and simultaneously decreased nonshivering thermogenesis compared to those housed at 21°C...
November 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27411567/dna-barcoding-of-formalin-fixed-aquatic-oligochaetes-for-biomonitoring
#2
Régis Vivien, Benoit J D Ferrari, Jan Pawlowski
BACKGROUND: Oligochaetes are valuable bioindicators of the quality of watercourse and lake sediments. The morphological identification of aquatic oligochaetes is difficult, prompting the development of new molecular oligochaete indices based on DNA barcoding and Next-generation sequencing of sorted specimens. In general, the samples for DNA barcoding are fixed in absolute ethanol. However, in the case of aquatic oligochaetes, this medium is not appropriate as it can induce a modification of specimen abundances and of the composition of communities...
2016: BMC Research Notes
https://www.readbyqxmd.com/read/26866802/next-generation-sequencing-of-aquatic-oligochaetes-comparison-of-experimental-communities
#3
Régis Vivien, Franck Lejzerowicz, Jan Pawlowski
Aquatic oligochaetes are a common group of freshwater benthic invertebrates known to be very sensitive to environmental changes and currently used as bioindicators in some countries. However, more extensive application of oligochaetes for assessing the ecological quality of sediments in watercourses and lakes would require overcoming the difficulties related to morphology-based identification of oligochaetes species. This study tested the Next-Generation Sequencing (NGS) of a standard cytochrome c oxydase I (COI) barcode as a tool for the rapid assessment of oligochaete diversity in environmental samples, based on mixed specimen samples...
2016: PloS One
https://www.readbyqxmd.com/read/26612498/first-molecular-evidence-of-the-simultaneous-human-infection-with-two-species-of-echinococcus-granulosus-sensu-lato-echinococcus-granulosus-sensu-stricto-and-echinococcus-canadensis
#4
Myriam Oudni-M'rad, Selim M'rad, Amine Ksia, Rachida Lamiri, Mongi Mekki, Abdellatif Nouri, Habib Mezhoud, Hamouda Babba
Cystic echinococcosis is a widespread zoonotic parasitic disease especially in Tunisia which is one of the most endemic countries in the Mediterranean area. The etiological agent, Echinococcus granulosus sensu lato, implies dogs and other canids as definitive hosts and different herbivore species as intermediate hosts. Human contamination occurs during the consumption of parasite eggs passed in the environment through canid feces. Hydatid cysts coming from a child operated for multiple echinococcosis were collected and analyzed in order to genotype and to obtain some epidemiological molecular information...
March 2016: Parasitology Research
https://www.readbyqxmd.com/read/25447828/first-overview-of-the-culicoides-latreille-diptera-ceratopogonidae-livestock-associated-species-of-reunion-island-indian-ocean
#5
MULTICENTER STUDY
A Desvars, Y Grimaud, H Guis, O Esnault, X Allène, L Gardès, T Balenghien, T Baldet, J C Delécolle, C Garros
This study establishes the first faunistic inventory of livestock associated Culicoides (Diptera: Ceratopogonidae) species of Reunion Island (Indian Ocean), where bluetongue and epizootic hemorrhagic disease are regularly recorded. Single night-catches were performed at 41 sites using light suction traps at altitudes ranging from 0 to 1525 m, from March to April 2005. Five species were recorded: Culicoides imicola, Culicoides bolitinos, Culicoides enderleini, Culicoides grahamii, and Culicoides kibatiensis, among which at least the first three species are known to be involved in virus transmission to ruminants and equids...
February 2015: Acta Tropica
https://www.readbyqxmd.com/read/25299315/selected-case-from-the-arkadi-m-rywlin-international-pathology-slide-series-mitochondrial-myopathy-presenting-with-chronic-progressive-external-ophthalmoplegia-cpeo-a-case-report
#6
Michele Bisceglia, Paola Crociani, Danilo Fogli, Antonio Centola, Carlos A Galliani, Gianandrea Pasquinelli
A 43-year-old female patient diagnosed with chronic progressive external ophthalmoplegia (CPEO) because of mitochondrial myopathy documented by muscle biopsy is presented. The chief complaints were represented by blepharoptosis and ophthalmoplegia. The muscle biopsy was evaluated by histology, using the appropriate histochemical and histoenzimological stains. Ragged red fibers with Gomori trichrome stain were seen, which showed cytochrome c oxydase deficiency and abnormal succinate dehydrogenase staining in around 20% of muscle fibres...
November 2014: Advances in Anatomic Pathology
https://www.readbyqxmd.com/read/25208249/genetic-uniformity-and-long-distance-clonal-dispersal-in-the-invasive-androgenetic-corbicula-clams
#7
Lise-Marie Pigneur, Emilie Etoundi, David C Aldridge, Jonathan Marescaux, Nina Yasuda, Karine Van Doninck
The clam genus Corbicula is an interesting model system to study the evolution of reproductive modes as it includes both sexual and asexual (androgenetic) lineages. While the sexual populations are restricted to the native Asian areas, the androgenetic lineages are widely distributed being also found in America and Europe where they form a major aquatic invasive pest. We investigated the genetic diversity of native and invasive Corbicula populations through a worldwide sampling. The use of mitochondrial and nuclear (microsatellite) markers revealed an extremely low diversity in the invasive populations with only four, undiversified, genetic lineages distributed across Europe and America...
October 2014: Molecular Ecology
https://www.readbyqxmd.com/read/24655492/mitochondrial-protein-synthesis-is-increased-in-oxidative-skeletal-muscles-of-rats-with-cardiac-cachexia
#8
Ronan Thibault, Sylvain Chanséaume, Kasra Azarnoush, Christelle Guillet, Christophe Giraudet, Véronique Patrac, Jean-René Lusson, Noël Cano, Yves Boirie, Stéphane Walrand
Since cardiac cachexia could be associated with alterations in muscular mitochondrial metabolism, we hypothesized that the expected alterations in the activities of mitochondrial oxidative enzymes could be associated with changes in mitochondrial protein synthesis in oxidative skeletal muscles. Cardiac cachexia was provoked in male rats by the ligation of the left coronary artery. Six cachectic and 6 control rats were age-paired, and their food intake was observed. The synthesis of mitochondrial proteins was measured by [1-13C]-valine infusion in soleus, tibilais, myocardium, and liver...
March 2014: Nutrition Research
https://www.readbyqxmd.com/read/24415689/-body-mass-energy-budget-and-leptin-of-mice-under-stochastic-food-restriction-and-refeeding
#9
Zhi-Jun Zhao, Wen-Tao Wei, Ming-Zhen Li, Jing Cao
Periods of restricted food intake that lead to lower body weight are often followed by rapid regaining of the lost weight after ad libitum refeeding, an event generally known as the "compensatory growth". To explore the physiological mechanisms underlying "compensatory growth", we evaluated a series of energetic parameters (energy intake, energy expenditure, body composition and serum leptin levels) of adult KM mice subjected to three cycles of stochastic food restriction following by ad libitum refeeding (SFR-Re)...
December 2013: Dong Wu Xue Yan Jiu, Zoological Research
https://www.readbyqxmd.com/read/24365975/role-of-endothelial-nitric-oxide-in-pulmonary-and-systemic-arteries-during-hypoxia
#10
Cristina Nuñez, Victor M Victor, Miguel Martí, Pilar D'Ocon
UNLABELLED: Our aim was to investigate the role played by endothelial nitric oxide (NO) during acute vascular response to hypoxia, as a modulator of both vascular tone (through guanylate cyclase (sGC) activation) and mitochondrial O2 consumption (through competitive inhibition of cytochrome-c-oxydase (CcO)). Organ bath experiments were performed and O2 consumption (Clark electrode) was determined in isolated aorta, mesenteric and pulmonary arteries of rats and eNOS-knockout mice. All pre-contracted vessels exhibited a triphasic hypoxic response consisting of an initial transient contraction (not observed in vessels from eNOS-knockout mice) followed by relaxation and subsequent sustained contraction...
February 15, 2014: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/24349217/colonisation-and-diversification-of-the-zenaida-dove-zenaida-aurita-in-the-antilles-phylogeography-contemporary-gene-flow-and-morphological-divergence
#11
Karine Monceau, Frank Cézilly, Jérôme Moreau, Sébastien Motreuil, Rémi Wattier
Caribbean avifaunal biogeography has been mainly studied based on mitochondrial DNA. Here, we investigated both past and recent island differentiation and micro-evolutionary changes in the Zenaida Dove (Zenaida aurita) based on combined information from one mitochondrial (Cytochrome c Oxydase subunit I, COI) and 13 microsatellite markers and four morphological characters. This Caribbean endemic and abundant species has a large distribution, and two subspecies are supposed to occur: Z. a. zenaida in the Greater Antilles (GA) and Z...
2013: PloS One
https://www.readbyqxmd.com/read/23274094/hepatic-lipid-metabolic-pathways-modified-by-resveratrol-in-rats-fed-an-obesogenic-diet
#12
Goiuri Alberdi, Víctor M Rodríguez, M Teresa Macarulla, Jonatan Miranda, Itziar Churruca, María P Portillo
OBJECTIVE: The scientific community is on the look-out for safe biomolecules useful in the prevention of obesity and related aberrations such as fatty liver. This study analyzed the influence of resveratrol on hepatic triacylglycerol metabolism. METHODS: Male Sprague-Dawley rats were divided into control and resveratrol-treated groups (30 mg/kg of body weight per day) and fed a commercial obesogenic diet for 6 wk. Liver triacylglycerol content and the activity of carnitine palmitoyl transferase-Ia (CPT-Ia), acyl-coenzyme A oxydase (ACO), fatty acid synthase (FAS), glucose-6-phosphate dehydrogenase (G6PDH), malic enzyme (ME), acetyl-coenzyme A carboxylase (ACC), adenosine monophosphate-activated protein kinase (AMPK), and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) activation were measured...
March 2013: Nutrition
https://www.readbyqxmd.com/read/23227713/-the-state-of-the-cytochrome-part-of-respiratory-chain-in-tumor-carrier-rats-liver-in-the-conditions-of-preliminary-low-level-irradiation
#13
M M Marchenko, O N Voloshchuk
The effect of low-level irradiation on the structural and functional organization of the cytochrome part of the respiratory chain in tumor carrier rats' liver is studied. The preliminary low-level irradiation leading to the mitochondrial cytochrome a, b and c content reduction at the latent stage of Guerin's carcinoma is shown. At the same time, the maximal reduction of the content of all liver cytochromes is observed at the terminal stages of oncogenesis. The content of cytochome c undergoes the most significant changes in the liver mitochondrial fracture...
September 2012: Radiatsionnaia Biologiia, Radioecologiia
https://www.readbyqxmd.com/read/23138562/variation-patterns-in-individual-fish-responses-to-chemical-stress-among-estuaries-seasons-and-genders-the-case-of-the-european-flounder-platichthys-flesus-in-the-bay-of-biscay
#14
Jean Laroche, Olivier Gauthier, Louis Quiniou, Alain Devaux, Sylvie Bony, Estérine Evrard, Jérôme Cachot, Yan Chérel, Thibaut Larcher, Ricardo Riso, Vianney Pichereau, Marie Hélène Devier, Hélène Budzinski
The objective was to describe and model variation patterns in individual fish responses to contaminants among estuaries, season and gender. Two hundred twenty-seven adult European flounders were collected in two seasons (winter and summer) in four estuaries along the Bay of Biscay (South West France), focusing on a pristine system (the Ster), vs. three estuaries displaying contrasted levels of contaminants (the Vilaine, Loire and Gironde). Twenty-three variables were measured by fish, considering the load of contaminants (liver metals, liver and muscle persistent organic pollutants, muscle polycyclic aromatic hydrocarbons); the gene expression (Cyt C oxydase, ATPase, BHMT, Cyt P450 1A1, ferritin); the blood genotoxicity (Comet test); and liver histology (foci of cellular alteration-tumour, steatosis, inflammation, abnormal glycogen storage)...
February 2013: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/22004100/identification-of-molecular-markers-for-dna-barcoding-in-the-aphidiinae-hym-braconidae
#15
Stephane A P Derocles, Anne LE Ralec, Manuel Plantegenest, Bernard Chaubet, Corinne Cruaud, Astrid Cruaud, Jean-Yves Rasplus
Reliable identification of Aphidiinae species (Braconidae) is a prerequisite for conducting studies on aphid-parasitoid interactions at the community level. However, morphological identification of Aphidiinae species remains problematic even for specialists and is almost impossible with larval stages. Here, we compared the efficiency of two molecular markers [mitochondrial cytochrome c oxydase I (COI) and nuclear long wavelength rhodopsin (LWRh)] that could be used to accurately identify about 50 species of Aphidiinae that commonly occur in aphid-parasitoid networks in northwestern Europe...
March 2012: Molecular Ecology Resources
https://www.readbyqxmd.com/read/21952956/haplotype-identification-within-spodoptera-frugiperda-j-e-smith-lepidoptera-noctuidae-corn-and-rice-strains-from-colombia
#16
COMPARATIVE STUDY
H Salinas-Hernandez, C I Saldamando-Benjumea
The fall army worm Spodoptera frugiperda (Smith) is a migratory important pest of corn, sorghum, rice, grass and bermudagrass in North and South America. This species has diverged into two genetically differentiated but morphologically identical strains, "the rice" and "the corn". They have been analyzed by sequencing the genes cytochrome oxydase I, II and ITS1 from populations from the United States and Brazil. However, no such studies were performed in Colombia. In here, we identified 43 haplotypes by sequencing a fragment of the COI gene from 102 individuals, of which 40 had already been identified as the "corn" and "rice" strains or to their hybrids from Tolima, and the rest were collected from corn, cotton, sorghum, grass and rice fields in other regions of Colombia...
July 2011: Neotropical Entomology
https://www.readbyqxmd.com/read/21938590/misidentification-of-olga-ph-j-92-believed-to-be-the-only-crustacean-cell-line
#17
Lucy E J Lee, Mary Rose Bufalino, Andrew E Christie, Marc E Frischer, Thomas Soin, Clement K M Tsui, Robert H Hanner, Guy Smagghe
Continuous cell lines from aquatic invertebrate species are few and the development of crustacean cell lines remains an elusive goal. Although a crayfish cell line derived from neural ganglia of Orconectes limosus was reported in 2000, this cell line OLGA-PH-J/92 failed to be authenticated as such. In this report, we describe our attempts to identify the taxonomic identity of the cell line through immunological and molecular techniques. Immunohistochemical screening for the expression of a suite of invertebrate neuropeptides gave negative results, precluding an invertebrate neural origin...
October 2011: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/21082238/evidence-for-ectopic-aerobic-atp-production-on-c6-glioma-cell-plasma-membrane
#18
Silvia Ravera, Maria Grazia Aluigi, Daniela Calzia, Paola Ramoino, Alessandro Morelli, Isabella Panfoli
Extracellular ATP plays a pivotal role as a signaling molecule in physiological and pathological conditions in the CNS. In several glioma cell lines, ATP is a positive factor for one or more characteristics important for the abnormal growth and survival of these cells. This work presents immunofluorescence and biochemical analyses suggesting that an aerobic metabolism, besides mitochondria, is located also on the plasma membrane of C6 glioma cells. An ATP synthesis coupled to oxygen consumption was measured in plasma membrane isolated from C6 cells, sensitive to common inhibitors of respiratory chain complexes, suggesting the involvement of a putative surface ATP synthase complex...
March 2011: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/21038691/-effect-of-whisker-trimming-on-behavior-and-barrel-cortex-of-rat
#19
Man-Li Sun, Xiao-Bing Zhang, Xia Sun, Meng-Hui Zhao, Yan-Qin Yu
OBJECTIVE: To investigate the change of behavior, as well as the plasticity of somatosensory cortex after whisker trimming. METHODS: SD rats were divided into 4 groups. Group A is the normal control group; group B: bilateral vibrissotomy on the second postnatal day; group C: unilateral right vibrissotomy on the second postnatal day; group D: right unilateral whisker trimmed during 1-5 days after birth, and leave untreated after the 5th postnatal day. Their body weight, length of the left D2 whiskers was measured on the 30th postnatal day...
August 2010: Chinese Journal of Applied Physiology
https://www.readbyqxmd.com/read/20504385/multiplex-pcr-assay-to-discriminate-four-neighbouring-species-of-the-calliptamus-genus-orthoptera-acrididae-from-france
#20
E Blanchet, L Blondin, P A Gagnaire, A Foucart, J M Vassal, M Lecoq
Definition of the genus Calliptamus (Orthoptera: Acrididae) has generated many taxonomic debates. Even now, the existence of different geographical morphs hinders species determination, particularly as concerns females and larvae. Some of these species are observed in southern France and are recognized as potential pests. To circumvent problems of species identification in ecological surveys, we developed a single multiplex PCR method based on mitochondrial Cytochrome Oxydase I diagnostic polymorphisms to differentiate between the four species, Calliptamus italicus, C...
December 2010: Bulletin of Entomological Research
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